<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with Wdesk from Workiva-->
<!--Copyright 2023 Workiva-->
<!--r:49e2985d-331d-4826-b11f-14787eb7223a,g:77c4d7b1-27e6-488a-9ff7-9a2d5efc7319-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:roleRef roleURI="http://www.csx.com/role/CONSOLIDATEDINCOMESTATEMENTSUnaudited" xlink:type="simple" xlink:href="csx-20230331.xsd#CONSOLIDATEDINCOMESTATEMENTSUnaudited"/>
  <link:calculationLink xlink:role="http://www.csx.com/role/CONSOLIDATEDINCOMESTATEMENTSUnaudited" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_31b16d01-27fe-4494-8b1f-5bb6a10fc7db" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_bab7369b-8c7f-479b-a385-48cd4feb5f47" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingIncomeLoss_31b16d01-27fe-4494-8b1f-5bb6a10fc7db" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_bab7369b-8c7f-479b-a385-48cd4feb5f47" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingCostsAndExpenses_8d9fce86-5973-4f9d-b91d-bc0b446e29b0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingCostsAndExpenses"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingIncomeLoss_31b16d01-27fe-4494-8b1f-5bb6a10fc7db" xlink:to="loc_us-gaap_OperatingCostsAndExpenses_8d9fce86-5973-4f9d-b91d-bc0b446e29b0" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_4ef9667d-6f05-4373-83bd-dab8d162c1f3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonoperatingIncomeExpense_1e927f40-c66f-4d12-a9ed-01247e440437" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NonoperatingIncomeExpense"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_4ef9667d-6f05-4373-83bd-dab8d162c1f3" xlink:to="loc_us-gaap_NonoperatingIncomeExpense_1e927f40-c66f-4d12-a9ed-01247e440437" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestIncomeExpenseNet_bef63150-d6f8-4031-8682-878d493f3273" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InterestIncomeExpenseNet"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_4ef9667d-6f05-4373-83bd-dab8d162c1f3" xlink:to="loc_us-gaap_InterestIncomeExpenseNet_bef63150-d6f8-4031-8682-878d493f3273" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_bcf0490f-f974-462c-bf5a-0dc3cdd396d4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_4ef9667d-6f05-4373-83bd-dab8d162c1f3" xlink:to="loc_us-gaap_OperatingIncomeLoss_bcf0490f-f974-462c-bf5a-0dc3cdd396d4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingCostsAndExpenses_48905407-ed6d-4169-b259-631a8d1390a7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingCostsAndExpenses"/>
    <link:loc xlink:type="locator" xlink:label="loc_csx_EquipmentAndOtherRents_8e0889a3-d145-4d5b-bf3c-80b876b2ef9d" xlink:href="csx-20230331.xsd#csx_EquipmentAndOtherRents"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingCostsAndExpenses_48905407-ed6d-4169-b259-631a8d1390a7" xlink:to="loc_csx_EquipmentAndOtherRents_8e0889a3-d145-4d5b-bf3c-80b876b2ef9d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherCostAndExpenseOperating_04822ebc-3cf0-4b92-80e3-84670edfd044" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherCostAndExpenseOperating"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingCostsAndExpenses_48905407-ed6d-4169-b259-631a8d1390a7" xlink:to="loc_us-gaap_OtherCostAndExpenseOperating_04822ebc-3cf0-4b92-80e3-84670edfd044" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepreciationDepletionAndAmortization_eeb1243e-e126-45ee-bc94-30c0965680ad" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DepreciationDepletionAndAmortization"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingCostsAndExpenses_48905407-ed6d-4169-b259-631a8d1390a7" xlink:to="loc_us-gaap_DepreciationDepletionAndAmortization_eeb1243e-e126-45ee-bc94-30c0965680ad" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LaborAndRelatedExpense_80eea278-a9da-4c32-b5ad-87cb7430fef9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LaborAndRelatedExpense"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingCostsAndExpenses_48905407-ed6d-4169-b259-631a8d1390a7" xlink:to="loc_us-gaap_LaborAndRelatedExpense_80eea278-a9da-4c32-b5ad-87cb7430fef9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FuelCosts_af87344d-2dd3-47f0-b295-b8af827cc849" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FuelCosts"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingCostsAndExpenses_48905407-ed6d-4169-b259-631a8d1390a7" xlink:to="loc_us-gaap_FuelCosts_af87344d-2dd3-47f0-b295-b8af827cc849" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GainLossOnSaleOfPropertyPlantEquipment_d600a001-6e8f-4657-8076-7a8c81ad77f9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_GainLossOnSaleOfPropertyPlantEquipment"/>
    <link:calculationArc order="6" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingCostsAndExpenses_48905407-ed6d-4169-b259-631a8d1390a7" xlink:to="loc_us-gaap_GainLossOnSaleOfPropertyPlantEquipment_d600a001-6e8f-4657-8076-7a8c81ad77f9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_777c9e41-a7da-465a-825f-e7920c9f52f2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetIncomeLoss"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_f75b305c-5716-4528-aef4-f0107afe136b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_777c9e41-a7da-465a-825f-e7920c9f52f2" xlink:to="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_f75b305c-5716-4528-aef4-f0107afe136b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_2844e112-65d5-47f7-ab8f-51b3611fd1b4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncomeTaxExpenseBenefit"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_777c9e41-a7da-465a-825f-e7920c9f52f2" xlink:to="loc_us-gaap_IncomeTaxExpenseBenefit_2844e112-65d5-47f7-ab8f-51b3611fd1b4" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.csx.com/role/CONSOLIDATEDBALANCESHEETS" xlink:type="simple" xlink:href="csx-20230331.xsd#CONSOLIDATEDBALANCESHEETS"/>
  <link:calculationLink xlink:role="http://www.csx.com/role/CONSOLIDATEDBALANCESHEETS" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_add6f226-eae6-4610-9f2c-b8c97144a8a9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_Liabilities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingencyAccrualCarryingValueNoncurrent_7db25672-3c5d-42e9-b5aa-779c10849026" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LossContingencyAccrualCarryingValueNoncurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_add6f226-eae6-4610-9f2c-b8c97144a8a9" xlink:to="loc_us-gaap_LossContingencyAccrualCarryingValueNoncurrent_7db25672-3c5d-42e9-b5aa-779c10849026" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilitiesNoncurrent_94f59f33-b8a0-4620-a71e-290a7f352995" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherLiabilitiesNoncurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_add6f226-eae6-4610-9f2c-b8c97144a8a9" xlink:to="loc_us-gaap_OtherLiabilitiesNoncurrent_94f59f33-b8a0-4620-a71e-290a7f352995" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtAndCapitalLeaseObligations_1e0e7123-1a1d-4ce5-9c4a-8b0b1180f130" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebtAndCapitalLeaseObligations"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_add6f226-eae6-4610-9f2c-b8c97144a8a9" xlink:to="loc_us-gaap_LongTermDebtAndCapitalLeaseObligations_1e0e7123-1a1d-4ce5-9c4a-8b0b1180f130" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_d5db2703-c7d7-4e58-9df1-5928cf153d9b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLeaseLiabilityNoncurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_add6f226-eae6-4610-9f2c-b8c97144a8a9" xlink:to="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_d5db2703-c7d7-4e58-9df1-5928cf153d9b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredIncomeTaxLiabilitiesNet_e5c8bf49-0d3a-4734-91a1-565f152a0d59" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DeferredIncomeTaxLiabilitiesNet"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_add6f226-eae6-4610-9f2c-b8c97144a8a9" xlink:to="loc_us-gaap_DeferredIncomeTaxLiabilitiesNet_e5c8bf49-0d3a-4734-91a1-565f152a0d59" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_09711ca4-e4f3-4b17-a5f1-af9622e6e8fa" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LiabilitiesCurrent"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_add6f226-eae6-4610-9f2c-b8c97144a8a9" xlink:to="loc_us-gaap_LiabilitiesCurrent_09711ca4-e4f3-4b17-a5f1-af9622e6e8fa" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquity_5c02464f-880b-4150-a2ae-edbd25a18b43" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LiabilitiesAndStockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_93970188-2f01-4c79-9dc3-0f11c81a58b9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_5c02464f-880b-4150-a2ae-edbd25a18b43" xlink:to="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_93970188-2f01-4c79-9dc3-0f11c81a58b9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_a063e159-ba0f-4e41-8c00-8a6fa743bda3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_Liabilities"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_5c02464f-880b-4150-a2ae-edbd25a18b43" xlink:to="loc_us-gaap_Liabilities_a063e159-ba0f-4e41-8c00-8a6fa743bda3" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_415a7cfa-991c-46e2-ab1f-4769cba889d6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LiabilitiesCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsPayableCurrent_a4f0006d-ac25-42ea-b9eb-5cc447602a99" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccountsPayableCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_415a7cfa-991c-46e2-ab1f-4769cba889d6" xlink:to="loc_us-gaap_AccountsPayableCurrent_a4f0006d-ac25-42ea-b9eb-5cc447602a99" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeRelatedLiabilitiesCurrent_a3523527-c304-4337-8471-c19d20a33c6b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EmployeeRelatedLiabilitiesCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_415a7cfa-991c-46e2-ab1f-4769cba889d6" xlink:to="loc_us-gaap_EmployeeRelatedLiabilitiesCurrent_a3523527-c304-4337-8471-c19d20a33c6b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingencyAccrualCarryingValueCurrent_fc83f769-5ca0-4343-8477-6721bfbcfea8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LossContingencyAccrualCarryingValueCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_415a7cfa-991c-46e2-ab1f-4769cba889d6" xlink:to="loc_us-gaap_LossContingencyAccrualCarryingValueCurrent_fc83f769-5ca0-4343-8477-6721bfbcfea8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtAndCapitalLeaseObligationsCurrent_3230e9e4-90ea-4d5a-b1a2-0503cfcfb7a0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebtAndCapitalLeaseObligationsCurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_415a7cfa-991c-46e2-ab1f-4769cba889d6" xlink:to="loc_us-gaap_LongTermDebtAndCapitalLeaseObligationsCurrent_3230e9e4-90ea-4d5a-b1a2-0503cfcfb7a0" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TaxesPayableCurrent_d33ada57-3cba-4727-872c-5c1506772148" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TaxesPayableCurrent"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_415a7cfa-991c-46e2-ab1f-4769cba889d6" xlink:to="loc_us-gaap_TaxesPayableCurrent_d33ada57-3cba-4727-872c-5c1506772148" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilitiesCurrent_d4077aa4-ca00-40c6-89ff-ce4745f45c82" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherLiabilitiesCurrent"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_415a7cfa-991c-46e2-ab1f-4769cba889d6" xlink:to="loc_us-gaap_OtherLiabilitiesCurrent_d4077aa4-ca00-40c6-89ff-ce4745f45c82" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_d57535a1-78fa-43d1-8247-5160dc396a0f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MinorityInterest_708e11df-3a60-46a6-9e3b-4cb6421555ee" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_MinorityInterest"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_d57535a1-78fa-43d1-8247-5160dc396a0f" xlink:to="loc_us-gaap_MinorityInterest_708e11df-3a60-46a6-9e3b-4cb6421555ee" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_25a29f67-6c3a-45b9-bc3b-a17c60496161" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_d57535a1-78fa-43d1-8247-5160dc396a0f" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_25a29f67-6c3a-45b9-bc3b-a17c60496161" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsAccumulatedDeficit_bc8da04c-2864-42c3-930e-0e103b83ff79" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RetainedEarningsAccumulatedDeficit"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_d57535a1-78fa-43d1-8247-5160dc396a0f" xlink:to="loc_us-gaap_RetainedEarningsAccumulatedDeficit_bc8da04c-2864-42c3-930e-0e103b83ff79" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalCommonStock_8e8d47a3-b7ac-4ddb-9010-8a024b6097eb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AdditionalPaidInCapitalCommonStock"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_d57535a1-78fa-43d1-8247-5160dc396a0f" xlink:to="loc_us-gaap_AdditionalPaidInCapitalCommonStock_8e8d47a3-b7ac-4ddb-9010-8a024b6097eb" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockValue_624497ae-c3ea-43bb-8393-788fd307d9f2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommonStockValue"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_d57535a1-78fa-43d1-8247-5160dc396a0f" xlink:to="loc_us-gaap_CommonStockValue_624497ae-c3ea-43bb-8393-788fd307d9f2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_43229dcf-758c-48ff-b617-b27800be2175" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentGross_0c846007-3290-4384-882c-b145c70a7073" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentGross"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentNet_43229dcf-758c-48ff-b617-b27800be2175" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentGross_0c846007-3290-4384-882c-b145c70a7073" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_ad9f2b59-1f53-4161-8765-07937542b198" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentNet_43229dcf-758c-48ff-b617-b27800be2175" xlink:to="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_ad9f2b59-1f53-4161-8765-07937542b198" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Assets_c601bd9e-9668-4846-b8c5-fc5267002829" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_Assets"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IntangibleAssetsNetIncludingGoodwill_5e55e9c0-0135-4b71-8508-5de3ae9eca73" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IntangibleAssetsNetIncludingGoodwill"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_c601bd9e-9668-4846-b8c5-fc5267002829" xlink:to="loc_us-gaap_IntangibleAssetsNetIncludingGoodwill_5e55e9c0-0135-4b71-8508-5de3ae9eca73" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_fee696bd-1f3d-4015-8d6f-bc1f58d85830" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AssetsCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_c601bd9e-9668-4846-b8c5-fc5267002829" xlink:to="loc_us-gaap_AssetsCurrent_fee696bd-1f3d-4015-8d6f-bc1f58d85830" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_8a63adbc-c454-4b33-b0fa-8ecff626f59a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_c601bd9e-9668-4846-b8c5-fc5267002829" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_8a63adbc-c454-4b33-b0fa-8ecff626f59a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseRightOfUseAsset_71f4b8c4-ffcb-4c83-923b-5fff2eebc0cb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLeaseRightOfUseAsset"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_c601bd9e-9668-4846-b8c5-fc5267002829" xlink:to="loc_us-gaap_OperatingLeaseRightOfUseAsset_71f4b8c4-ffcb-4c83-923b-5fff2eebc0cb" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentsInAffiliatesSubsidiariesAssociatesAndJointVentures_74302c00-3da6-4af2-99f5-e1ba5aa65d07" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InvestmentsInAffiliatesSubsidiariesAssociatesAndJointVentures"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_c601bd9e-9668-4846-b8c5-fc5267002829" xlink:to="loc_us-gaap_InvestmentsInAffiliatesSubsidiariesAssociatesAndJointVentures_74302c00-3da6-4af2-99f5-e1ba5aa65d07" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsNoncurrent_706944c5-1027-45e4-99d2-0f75bc1365a7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherAssetsNoncurrent"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_c601bd9e-9668-4846-b8c5-fc5267002829" xlink:to="loc_us-gaap_OtherAssetsNoncurrent_706944c5-1027-45e4-99d2-0f75bc1365a7" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_a9d733db-39b6-4fc7-9185-ca09ed16c77a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AssetsCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_aafbc23e-b1be-4742-9ca2-901390a355a2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_a9d733db-39b6-4fc7-9185-ca09ed16c77a" xlink:to="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_aafbc23e-b1be-4742-9ca2-901390a355a2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsReceivableNetCurrent_922d6d93-6b25-4d24-bd1a-090c2603b476" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccountsReceivableNetCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_a9d733db-39b6-4fc7-9185-ca09ed16c77a" xlink:to="loc_us-gaap_AccountsReceivableNetCurrent_922d6d93-6b25-4d24-bd1a-090c2603b476" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MaterialsSuppliesAndOther_a68bcf67-4056-4459-9648-08cb44014447" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_MaterialsSuppliesAndOther"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_a9d733db-39b6-4fc7-9185-ca09ed16c77a" xlink:to="loc_us-gaap_MaterialsSuppliesAndOther_a68bcf67-4056-4459-9648-08cb44014447" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsCurrent_d6dc25f1-e98b-4835-b560-e6d440567385" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherAssetsCurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_a9d733db-39b6-4fc7-9185-ca09ed16c77a" xlink:to="loc_us-gaap_OtherAssetsCurrent_d6dc25f1-e98b-4835-b560-e6d440567385" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShortTermInvestments_0d4d987d-1aab-4fea-a88c-b388a186b01e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShortTermInvestments"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_a9d733db-39b6-4fc7-9185-ca09ed16c77a" xlink:to="loc_us-gaap_ShortTermInvestments_0d4d987d-1aab-4fea-a88c-b388a186b01e" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.csx.com/role/CONSOLIDATEDCASHFLOWSTATEMENTSUnaudited" xlink:type="simple" xlink:href="csx-20230331.xsd#CONSOLIDATEDCASHFLOWSTATEMENTSUnaudited"/>
  <link:calculationLink xlink:role="http://www.csx.com/role/CONSOLIDATEDCASHFLOWSTATEMENTSUnaudited" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_fcf49fb1-f8a5-4347-b29c-d4be98e796bd" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_4d6b957d-29a0-4a55-97d8-a5205f008200" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetIncomeLoss"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_fcf49fb1-f8a5-4347-b29c-d4be98e796bd" xlink:to="loc_us-gaap_NetIncomeLoss_4d6b957d-29a0-4a55-97d8-a5205f008200" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepreciationDepletionAndAmortization_9eb1258e-df93-4a92-b3d4-4cb7c7518729" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DepreciationDepletionAndAmortization"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_fcf49fb1-f8a5-4347-b29c-d4be98e796bd" xlink:to="loc_us-gaap_DepreciationDepletionAndAmortization_9eb1258e-df93-4a92-b3d4-4cb7c7518729" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredIncomeTaxExpenseBenefit_bf2a64ad-32ce-45a7-8b11-2a535d4766f4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DeferredIncomeTaxExpenseBenefit"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_fcf49fb1-f8a5-4347-b29c-d4be98e796bd" xlink:to="loc_us-gaap_DeferredIncomeTaxExpenseBenefit_bf2a64ad-32ce-45a7-8b11-2a535d4766f4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GainLossOnSaleOfPropertyPlantEquipment_d3430557-b2b5-4a46-a18c-813a59d32162" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_GainLossOnSaleOfPropertyPlantEquipment"/>
    <link:calculationArc order="4" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_fcf49fb1-f8a5-4347-b29c-d4be98e796bd" xlink:to="loc_us-gaap_GainLossOnSaleOfPropertyPlantEquipment_d3430557-b2b5-4a46-a18c-813a59d32162" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherOperatingActivitiesCashFlowStatement_ef78261e-6c84-4b88-ab67-41c7d303b557" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherOperatingActivitiesCashFlowStatement"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_fcf49fb1-f8a5-4347-b29c-d4be98e796bd" xlink:to="loc_us-gaap_OtherOperatingActivitiesCashFlowStatement_ef78261e-6c84-4b88-ab67-41c7d303b557" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsReceivable_8f2ba7bd-6f50-42b9-be03-75a36af1eede" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInAccountsReceivable"/>
    <link:calculationArc order="6" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_fcf49fb1-f8a5-4347-b29c-d4be98e796bd" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsReceivable_8f2ba7bd-6f50-42b9-be03-75a36af1eede" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInOtherCurrentAssets_ad3263a9-7c9b-4119-bd2c-94f73bb00012" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInOtherCurrentAssets"/>
    <link:calculationArc order="7" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_fcf49fb1-f8a5-4347-b29c-d4be98e796bd" xlink:to="loc_us-gaap_IncreaseDecreaseInOtherCurrentAssets_ad3263a9-7c9b-4119-bd2c-94f73bb00012" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsPayable_be56d22c-86e8-4f36-88e5-53bb6f73dcc1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInAccountsPayable"/>
    <link:calculationArc order="8" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_fcf49fb1-f8a5-4347-b29c-d4be98e796bd" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsPayable_be56d22c-86e8-4f36-88e5-53bb6f73dcc1" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccruedTaxesPayable_74c19398-07c7-420e-a040-71b496b5fbba" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInAccruedTaxesPayable"/>
    <link:calculationArc order="9" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_fcf49fb1-f8a5-4347-b29c-d4be98e796bd" xlink:to="loc_us-gaap_IncreaseDecreaseInAccruedTaxesPayable_74c19398-07c7-420e-a040-71b496b5fbba" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInOtherCurrentLiabilities_1905f93a-8372-4c19-9e97-749d3f5fbe98" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInOtherCurrentLiabilities"/>
    <link:calculationArc order="10" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_fcf49fb1-f8a5-4347-b29c-d4be98e796bd" xlink:to="loc_us-gaap_IncreaseDecreaseInOtherCurrentLiabilities_1905f93a-8372-4c19-9e97-749d3f5fbe98" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_167a0c7d-d446-4499-9888-b1743a522866" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsOfDividendsCommonStock_90b0cce5-e2d3-4849-af34-767da0ee05fd" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PaymentsOfDividendsCommonStock"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_167a0c7d-d446-4499-9888-b1743a522866" xlink:to="loc_us-gaap_PaymentsOfDividendsCommonStock_90b0cce5-e2d3-4849-af34-767da0ee05fd" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RepaymentsOfLongTermDebt_d192fab3-a067-4729-9504-9ca7c9892ccf" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RepaymentsOfLongTermDebt"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_167a0c7d-d446-4499-9888-b1743a522866" xlink:to="loc_us-gaap_RepaymentsOfLongTermDebt_d192fab3-a067-4729-9504-9ca7c9892ccf" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsForRepurchaseOfCommonStock_1fa06bfa-7037-42fc-ae69-664564231445" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PaymentsForRepurchaseOfCommonStock"/>
    <link:calculationArc order="3" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_167a0c7d-d446-4499-9888-b1743a522866" xlink:to="loc_us-gaap_PaymentsForRepurchaseOfCommonStock_1fa06bfa-7037-42fc-ae69-664564231445" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromPaymentsForOtherFinancingActivities_d6903dd9-60c5-4abe-b38b-4805722f6346" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ProceedsFromPaymentsForOtherFinancingActivities"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_167a0c7d-d446-4499-9888-b1743a522866" xlink:to="loc_us-gaap_ProceedsFromPaymentsForOtherFinancingActivities_d6903dd9-60c5-4abe-b38b-4805722f6346" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_4bcb61b6-0128-4381-95cb-d7e3c2f15eca" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_4ab7c318-2e3e-44c9-bb93-5584e81f1d0e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_4bcb61b6-0128-4381-95cb-d7e3c2f15eca" xlink:to="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_4ab7c318-2e3e-44c9-bb93-5584e81f1d0e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_c110068c-9252-4b64-8023-c59e87b10cf2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_4bcb61b6-0128-4381-95cb-d7e3c2f15eca" xlink:to="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_c110068c-9252-4b64-8023-c59e87b10cf2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_09ddaa76-c5f5-4bb7-835f-f856c9586cd5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_4bcb61b6-0128-4381-95cb-d7e3c2f15eca" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_09ddaa76-c5f5-4bb7-835f-f856c9586cd5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_0286bee0-97aa-4a64-8bc3-741738f5ebcb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireShortTermInvestments_96dc4f1c-619e-41ea-abfa-c498baa8df45" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PaymentsToAcquireShortTermInvestments"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_0286bee0-97aa-4a64-8bc3-741738f5ebcb" xlink:to="loc_us-gaap_PaymentsToAcquireShortTermInvestments_96dc4f1c-619e-41ea-abfa-c498baa8df45" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_6e90a7f9-f40e-4d9c-8505-17cf8124597f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PaymentsToAcquirePropertyPlantAndEquipment"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_0286bee0-97aa-4a64-8bc3-741738f5ebcb" xlink:to="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_6e90a7f9-f40e-4d9c-8505-17cf8124597f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireBusinessesNetOfCashAcquired_b621edfa-af38-4732-b739-ec0642370ab9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PaymentsToAcquireBusinessesNetOfCashAcquired"/>
    <link:calculationArc order="3" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_0286bee0-97aa-4a64-8bc3-741738f5ebcb" xlink:to="loc_us-gaap_PaymentsToAcquireBusinessesNetOfCashAcquired_b621edfa-af38-4732-b739-ec0642370ab9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsForProceedsFromOtherInvestingActivities_7f21697b-2fd9-4c34-86b6-92384c2450e4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PaymentsForProceedsFromOtherInvestingActivities"/>
    <link:calculationArc order="4" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_0286bee0-97aa-4a64-8bc3-741738f5ebcb" xlink:to="loc_us-gaap_PaymentsForProceedsFromOtherInvestingActivities_7f21697b-2fd9-4c34-86b6-92384c2450e4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromSaleOfShortTermInvestments_a687ffd1-f465-46c0-ae77-b48b299f5314" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ProceedsFromSaleOfShortTermInvestments"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_0286bee0-97aa-4a64-8bc3-741738f5ebcb" xlink:to="loc_us-gaap_ProceedsFromSaleOfShortTermInvestments_a687ffd1-f465-46c0-ae77-b48b299f5314" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromSaleOfPropertyPlantAndEquipment_d03f1962-8c38-4066-8366-533192af3aac" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ProceedsFromSaleOfPropertyPlantAndEquipment"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_0286bee0-97aa-4a64-8bc3-741738f5ebcb" xlink:to="loc_us-gaap_ProceedsFromSaleOfPropertyPlantAndEquipment_d03f1962-8c38-4066-8366-533192af3aac" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.csx.com/role/EarningsPerShareComputationofBasicEarningsPerShareAssumingDilutionDetails" xlink:type="simple" xlink:href="csx-20230331.xsd#EarningsPerShareComputationofBasicEarningsPerShareAssumingDilutionDetails"/>
  <link:calculationLink xlink:role="http://www.csx.com/role/EarningsPerShareComputationofBasicEarningsPerShareAssumingDilutionDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_ff7c29ab-ae4a-4c29-90a9-3efd9225b9f5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_7e6962d6-c7cb-4451-b593-84c761aa3772" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_WeightedAverageNumberOfSharesOutstandingBasic"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_ff7c29ab-ae4a-4c29-90a9-3efd9225b9f5" xlink:to="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_7e6962d6-c7cb-4451-b593-84c761aa3772" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberDilutedSharesOutstandingAdjustment_17aea380-30ce-43a6-b65d-2c6bf2b0283f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_WeightedAverageNumberDilutedSharesOutstandingAdjustment"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_ff7c29ab-ae4a-4c29-90a9-3efd9225b9f5" xlink:to="loc_us-gaap_WeightedAverageNumberDilutedSharesOutstandingAdjustment_17aea380-30ce-43a6-b65d-2c6bf2b0283f" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.csx.com/role/CasualtyEnvironmentalandOtherReservesScheduleofCasualtyEnvironmentalandOtherReservesDetails" xlink:type="simple" xlink:href="csx-20230331.xsd#CasualtyEnvironmentalandOtherReservesScheduleofCasualtyEnvironmentalandOtherReservesDetails"/>
  <link:calculationLink xlink:role="http://www.csx.com/role/CasualtyEnvironmentalandOtherReservesScheduleofCasualtyEnvironmentalandOtherReservesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingencyAccrualAtCarryingValue_1574bf5f-8082-4ba9-b411-4cdad679f51d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LossContingencyAccrualAtCarryingValue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingencyAccrualCarryingValueCurrent_53558fc4-5729-4ed0-a693-4e3e4b425017" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LossContingencyAccrualCarryingValueCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LossContingencyAccrualAtCarryingValue_1574bf5f-8082-4ba9-b411-4cdad679f51d" xlink:to="loc_us-gaap_LossContingencyAccrualCarryingValueCurrent_53558fc4-5729-4ed0-a693-4e3e4b425017" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingencyAccrualCarryingValueNoncurrent_075df6a1-a2c3-40e6-9338-b004e4df8e42" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LossContingencyAccrualCarryingValueNoncurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LossContingencyAccrualAtCarryingValue_1574bf5f-8082-4ba9-b411-4cdad679f51d" xlink:to="loc_us-gaap_LossContingencyAccrualCarryingValueNoncurrent_075df6a1-a2c3-40e6-9338-b004e4df8e42" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.csx.com/role/EmployeeBenefitPlansComponentsofNetPeriodicBenefitCostsDetails" xlink:type="simple" xlink:href="csx-20230331.xsd#EmployeeBenefitPlansComponentsofNetPeriodicBenefitCostsDetails"/>
  <link:calculationLink xlink:role="http://www.csx.com/role/EmployeeBenefitPlansComponentsofNetPeriodicBenefitCostsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetPeriodicDefinedBenefitsExpenseReversalOfExpenseExcludingServiceCostComponent_3f75632a-96e7-4b74-a01b-af03cc48a573" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetPeriodicDefinedBenefitsExpenseReversalOfExpenseExcludingServiceCostComponent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanInterestCost_6bd58008-a5fe-473f-8579-7c7eeef746b7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DefinedBenefitPlanInterestCost"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetPeriodicDefinedBenefitsExpenseReversalOfExpenseExcludingServiceCostComponent_3f75632a-96e7-4b74-a01b-af03cc48a573" xlink:to="loc_us-gaap_DefinedBenefitPlanInterestCost_6bd58008-a5fe-473f-8579-7c7eeef746b7" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanExpectedReturnOnPlanAssets_55ec92ae-3fdc-4302-8ac5-7c83a2566265" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DefinedBenefitPlanExpectedReturnOnPlanAssets"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetPeriodicDefinedBenefitsExpenseReversalOfExpenseExcludingServiceCostComponent_3f75632a-96e7-4b74-a01b-af03cc48a573" xlink:to="loc_us-gaap_DefinedBenefitPlanExpectedReturnOnPlanAssets_55ec92ae-3fdc-4302-8ac5-7c83a2566265" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanAmortizationOfGainsLosses_33de1060-8f93-49a9-a571-e5c337e44b34" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DefinedBenefitPlanAmortizationOfGainsLosses"/>
    <link:calculationArc order="3" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetPeriodicDefinedBenefitsExpenseReversalOfExpenseExcludingServiceCostComponent_3f75632a-96e7-4b74-a01b-af03cc48a573" xlink:to="loc_us-gaap_DefinedBenefitPlanAmortizationOfGainsLosses_33de1060-8f93-49a9-a571-e5c337e44b34" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCost_5426fec6-cf26-42c7-a750-8b84e9e4a793" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DefinedBenefitPlanNetPeriodicBenefitCost"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetPeriodicDefinedBenefitsExpenseReversalOfExpenseExcludingServiceCostComponent_a825db5f-f1b2-4486-b06f-6c77f11d01cc" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetPeriodicDefinedBenefitsExpenseReversalOfExpenseExcludingServiceCostComponent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCost_5426fec6-cf26-42c7-a750-8b84e9e4a793" xlink:to="loc_us-gaap_NetPeriodicDefinedBenefitsExpenseReversalOfExpenseExcludingServiceCostComponent_a825db5f-f1b2-4486-b06f-6c77f11d01cc" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanServiceCost_61563416-7e58-4ef1-88d3-4d3577fe08bc" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DefinedBenefitPlanServiceCost"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCost_5426fec6-cf26-42c7-a750-8b84e9e4a793" xlink:to="loc_us-gaap_DefinedBenefitPlanServiceCost_61563416-7e58-4ef1-88d3-4d3577fe08bc" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.csx.com/role/DebtandCreditAgreementsActivityRelatedtoLongTermDebtDetails" xlink:type="simple" xlink:href="csx-20230331.xsd#DebtandCreditAgreementsActivityRelatedtoLongTermDebtDetails"/>
  <link:calculationLink xlink:role="http://www.csx.com/role/DebtandCreditAgreementsActivityRelatedtoLongTermDebtDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtAndCapitalLeaseObligations_354d4b93-b2d6-42e6-89a9-ff91ea921b1f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtAndCapitalLeaseObligations"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtAndCapitalLeaseObligationsCurrent_378ddd6c-4be7-42df-b1ca-56e9af13059e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebtAndCapitalLeaseObligationsCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DebtAndCapitalLeaseObligations_354d4b93-b2d6-42e6-89a9-ff91ea921b1f" xlink:to="loc_us-gaap_LongTermDebtAndCapitalLeaseObligationsCurrent_378ddd6c-4be7-42df-b1ca-56e9af13059e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtAndCapitalLeaseObligations_e4d85954-d495-4d2d-b2ff-147d563960d5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebtAndCapitalLeaseObligations"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DebtAndCapitalLeaseObligations_354d4b93-b2d6-42e6-89a9-ff91ea921b1f" xlink:to="loc_us-gaap_LongTermDebtAndCapitalLeaseObligations_e4d85954-d495-4d2d-b2ff-147d563960d5" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.csx.com/role/RevenuesAccountsReceivableNetDetails" xlink:type="simple" xlink:href="csx-20230331.xsd#RevenuesAccountsReceivableNetDetails"/>
  <link:calculationLink xlink:role="http://www.csx.com/role/RevenuesAccountsReceivableNetDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsReceivableNet_479cf2a2-ac44-4d70-9130-24d10f0c88a1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccountsReceivableNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsReceivableGross_ce4f1941-85f5-4cdd-ab92-e270b7da84eb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccountsReceivableGross"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccountsReceivableNet_479cf2a2-ac44-4d70-9130-24d10f0c88a1" xlink:to="loc_us-gaap_AccountsReceivableGross_ce4f1941-85f5-4cdd-ab92-e270b7da84eb" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllowanceForDoubtfulAccountsReceivable_5234da0a-af13-4064-b23c-7b5971aa5756" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AllowanceForDoubtfulAccountsReceivable"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccountsReceivableNet_479cf2a2-ac44-4d70-9130-24d10f0c88a1" xlink:to="loc_us-gaap_AllowanceForDoubtfulAccountsReceivable_5234da0a-af13-4064-b23c-7b5971aa5756" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.csx.com/role/FairValueMeasurementsInvestmentMaturitiesDetails" xlink:type="simple" xlink:href="csx-20230331.xsd#FairValueMeasurementsInvestmentMaturitiesDetails"/>
  <link:calculationLink xlink:role="http://www.csx.com/role/FairValueMeasurementsInvestmentMaturitiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_9038a33b-44f0-4595-9b17-2f7404c32145" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AvailableForSaleSecuritiesDebtSecurities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesRollingYearTwoThroughFiveFairValue_9bc8753d-39d7-43b1-b76f-dbdd9738bdba" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AvailableForSaleSecuritiesDebtMaturitiesRollingYearTwoThroughFiveFairValue"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_9038a33b-44f0-4595-9b17-2f7404c32145" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesRollingYearTwoThroughFiveFairValue_9bc8753d-39d7-43b1-b76f-dbdd9738bdba" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesNextRollingTwelveMonthsFairValue_4eb6405d-d2dc-483c-89bc-97cfb343717f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AvailableForSaleSecuritiesDebtMaturitiesNextRollingTwelveMonthsFairValue"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_9038a33b-44f0-4595-9b17-2f7404c32145" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesNextRollingTwelveMonthsFairValue_4eb6405d-d2dc-483c-89bc-97cfb343717f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesRollingAfterYearTenFairValue_5f5653a8-c665-4aae-a5a2-25704736a31b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AvailableForSaleSecuritiesDebtMaturitiesRollingAfterYearTenFairValue"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_9038a33b-44f0-4595-9b17-2f7404c32145" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesRollingAfterYearTenFairValue_5f5653a8-c665-4aae-a5a2-25704736a31b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesRollingYearSixThroughTenFairValue_9139a4b3-dfa0-479a-b6fc-d31626ac6c60" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AvailableForSaleSecuritiesDebtMaturitiesRollingYearSixThroughTenFairValue"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_9038a33b-44f0-4595-9b17-2f7404c32145" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesRollingYearSixThroughTenFairValue_9139a4b3-dfa0-479a-b6fc-d31626ac6c60" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.csx.com/role/OtherComprehensiveIncomeLossChangesinAOCIBalancebyComponentDetails" xlink:type="simple" xlink:href="csx-20230331.xsd#OtherComprehensiveIncomeLossChangesinAOCIBalancebyComponentDetails"/>
  <link:calculationLink xlink:role="http://www.csx.com/role/OtherComprehensiveIncomeLossChangesinAOCIBalancebyComponentDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_752c85d5-263b-44b9-8212-d72b094dc8bb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OciBeforeReclassificationsBeforeTaxAttributableToParent_46375b12-4b0a-418d-b50d-47b6b86fdc3f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OciBeforeReclassificationsBeforeTaxAttributableToParent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_752c85d5-263b-44b9-8212-d72b094dc8bb" xlink:to="loc_us-gaap_OciBeforeReclassificationsBeforeTaxAttributableToParent_46375b12-4b0a-418d-b50d-47b6b86fdc3f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReclassificationFromAociCurrentPeriodBeforeTaxAttributableToParent_fb554b85-5464-4a17-b13c-6b2128baecc2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ReclassificationFromAociCurrentPeriodBeforeTaxAttributableToParent"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_752c85d5-263b-44b9-8212-d72b094dc8bb" xlink:to="loc_us-gaap_ReclassificationFromAociCurrentPeriodBeforeTaxAttributableToParent_fb554b85-5464-4a17-b13c-6b2128baecc2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossTaxPortionAttributableToParent1_27d95e82-b61e-415d-9e4c-216d0a85d6d3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherComprehensiveIncomeLossTaxPortionAttributableToParent1"/>
    <link:calculationArc order="3" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_752c85d5-263b-44b9-8212-d72b094dc8bb" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossTaxPortionAttributableToParent1_27d95e82-b61e-415d-9e4c-216d0a85d6d3" xlink:type="arc"/>
  </link:calculationLink>
</link:linkbase>
