| | | | | | | | | | | |
| Geographic Location | Subsidiary Name | Year Incorporated | Jurisdiction of Incorporation |
| North America | | | |
| United States | Fastenal International Holdings Company | 1994 | Minnesota |
| Fastenal Company Purchasing | 1997 | Minnesota |
| Fastenal Company Leasing | 1997 | Minnesota |
| Fastenal IP Company | 2005 | Minnesota |
| Fastenal Air Fleet, LLC | 2006 | Minnesota |
| River Surplus and Supply, LLC | 2014 | Minnesota |
| Fastenal Mexico, LLC | 2016 | Minnesota |
| Innova Holdings, LLC | 2020 | Minnesota |
| Innova Supply Chain Solutions, LLC | 2020 | Minnesota |
| Canada | Fastenal Canada, Ltd. | 2008 | Canada |
| Mexico | Fastenal Mexico, S. de R.L. de C.V. | 1999 | Mexico |
| | | |
| Central & South America | | |
| Panama | Fastenal Panama, S.A. | 2009 | Panama |
| Brazil | Fastenal Brasil Importação, Exportação e Distribuição Ltda. | 2011 | Brazil |
| Fastenal Brasil Participacoes Ltda. | 2011 | Brazil |
| Chile | Fastenal Chile SpA | 2013 | Chile |
| | | |
| Asia | | | |
| Singapore | Fastenal Singapore Pte. Ltd. | 2001 | Singapore |
| China | Fastenal Asia Pacific Limited | 2003 | Hong Kong, China |
| FASTCO (Shanghai) Trading Co., Ltd. | 2003 | Shanghai, China |
| Fastenal (Shanghai) International Trading Co. Ltd. | 2012 | Shanghai, China |
| Fastenal (Tianjin) International Trading Co. Ltd. | 2012 | Tianjin, China |
| Fastenal (Shenzhen) International Trading Co. Ltd. | 2012 | Shenzhen, China |
| Malaysia | Fastenal Malaysia Sdn. Bhd. | 2009 | Malaysia |
| Thailand | Fastenal (Thailand) Ltd. | 2012 | Thailand |
| India | Fastenal India Sourcing IT and Procurement Private Ltd. | 2013 | India |
| Fastenal India Wholesale Private Ltd. | 2013 | India |
| | | |
| Europe | | | |
| The Netherlands | Fastenal Europe B.V. | 2003 | The Netherlands |
| Fastenal Netherlands Holdings B.V. | 2015 | The Netherlands |
| Hungary | Fastenal Europe Kft. | 2009 | Hungary |
| United Kingdom | Fastenal Europe Ltd. | 2010 | United Kingdom |
| Germany | Fastenal Europe GmbH | 2011 | Germany |
| Czech Republic | Fastenal Europe, s.r.o. | 2011 | Czech Republic |
| Italy | Fastenal Europe S.r.l. | 2011 | Italy |
| Romania | Fastenal Europe RO S.r.l. | 2012 | Romania |
| Sweden | Fastenal Europe AB | 2013 | Sweden |
| Poland | Fastenal Europe Sp. z o.o. | 2013 | Poland |
| Austria | Fastenal AT GmbH | 2016 | Austria |
| Switzerland | Fastenal Europe Sàrl | 2017 | Switzerland |
| Ireland | Fastenal Europe IE Limited | 2017 | Ireland |
| Spain | Fastenal Europe, S.L. | 2018 | Spain |
| France | Fastenal Europe FR Sàrl | 2018 | France |
| Belgium | Fastenal Europe BE BV | 2019 | Belgium |