<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2024 Workiva-->
<!--r:558b3b6f-1c24-4da0-8302-c443f02800b0,g:60191c6d-d8a9-4fba-b16f-f25a8761c6ee-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/DocumentAndEntityInformation" xlink:type="simple" xlink:href="lnt-20240331.xsd#DocumentAndEntityInformation"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/DocumentAndEntityInformation" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntitiesTable_65cbae7f-42ed-4a08-9584-2b4bd9d483d7" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntitiesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_EntitiesTable_65cbae7f-42ed-4a08-9584-2b4bd9d483d7" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_8a625d43-6ebf-4169-9d37-4cf13b939eea" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_dei_EntitiesTable_65cbae7f-42ed-4a08-9584-2b4bd9d483d7" xlink:to="loc_dei_LegalEntityAxis_8a625d43-6ebf-4169-9d37-4cf13b939eea" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_8a625d43-6ebf-4169-9d37-4cf13b939eea_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_8a625d43-6ebf-4169-9d37-4cf13b939eea" xlink:to="loc_dei_EntityDomain_8a625d43-6ebf-4169-9d37-4cf13b939eea_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_affbf737-8095-45f5-a2cf-d077565cdb90" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_8a625d43-6ebf-4169-9d37-4cf13b939eea" xlink:to="loc_dei_EntityDomain_affbf737-8095-45f5-a2cf-d077565cdb90" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_a59bed55-ed8d-4c03-8a25-682fadfa8a92" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_affbf737-8095-45f5-a2cf-d077565cdb90" xlink:to="loc_lnt_IplMember_a59bed55-ed8d-4c03-8a25-682fadfa8a92" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_d7df09a3-f391-4f4d-9912-6b164cd4e6bd" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_affbf737-8095-45f5-a2cf-d077565cdb90" xlink:to="loc_lnt_WplMember_d7df09a3-f391-4f4d-9912-6b164cd4e6bd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentType_66f1f14f-4930-458d-8a7e-8458cc04f4a6" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_DocumentType"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_DocumentType_66f1f14f-4930-458d-8a7e-8458cc04f4a6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentQuarterlyReport_8c78e773-2e9f-4bb9-b40b-c74e19c56d02" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_DocumentQuarterlyReport"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_DocumentQuarterlyReport_8c78e773-2e9f-4bb9-b40b-c74e19c56d02" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentPeriodEndDate_0ce5b665-1f6e-4c6e-b36e-88c7bcc808de" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_DocumentPeriodEndDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_DocumentPeriodEndDate_0ce5b665-1f6e-4c6e-b36e-88c7bcc808de" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentTransitionReport_9daab331-f50e-4aef-8ada-7626132e61d7" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_DocumentTransitionReport"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_DocumentTransitionReport_9daab331-f50e-4aef-8ada-7626132e61d7" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityRegistrantName_69667f57-4589-43de-a13c-e083b2878dac" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityRegistrantName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_EntityRegistrantName_69667f57-4589-43de-a13c-e083b2878dac" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCentralIndexKey_bef44dac-7ab2-43bd-b8a7-a06c8f341343" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityCentralIndexKey"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_EntityCentralIndexKey_bef44dac-7ab2-43bd-b8a7-a06c8f341343" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityIncorporationStateCountryCode_cd59cc9e-787f-4247-8588-f5ded3697b8b" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityIncorporationStateCountryCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_EntityIncorporationStateCountryCode_cd59cc9e-787f-4247-8588-f5ded3697b8b" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressAddressLine1_7be52e50-5ff1-4299-bf90-74ace6fb19fe" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityAddressAddressLine1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_EntityAddressAddressLine1_7be52e50-5ff1-4299-bf90-74ace6fb19fe" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressCityOrTown_4846e329-7b03-4d2b-9d4c-c5e3eee1e149" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityAddressCityOrTown"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_EntityAddressCityOrTown_4846e329-7b03-4d2b-9d4c-c5e3eee1e149" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressStateOrProvince_3f650a3e-c991-4263-a1f3-46bca9734ef7" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityAddressStateOrProvince"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_EntityAddressStateOrProvince_3f650a3e-c991-4263-a1f3-46bca9734ef7" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressPostalZipCode_eda79687-8e70-4610-9637-60f23c7c5f3c" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityAddressPostalZipCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_EntityAddressPostalZipCode_eda79687-8e70-4610-9637-60f23c7c5f3c" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CityAreaCode_b0e604c8-d283-490f-bb50-1c7acd85aaaa" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_CityAreaCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_CityAreaCode_b0e604c8-d283-490f-bb50-1c7acd85aaaa" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LocalPhoneNumber_f7e1f249-6b11-44c9-97dc-54114817933a" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LocalPhoneNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_LocalPhoneNumber_f7e1f249-6b11-44c9-97dc-54114817933a" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFileNumber_2fca065c-efa6-4012-bd23-85089e594690" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityFileNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_EntityFileNumber_2fca065c-efa6-4012-bd23-85089e594690" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityTaxIdentificationNumber_6ba9791c-005a-49bb-a133-8df85f2241eb" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityTaxIdentificationNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_EntityTaxIdentificationNumber_6ba9791c-005a-49bb-a133-8df85f2241eb" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_Security12bTitle_120b55ec-3904-45de-8c11-5350f7470dbb" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_Security12bTitle"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_Security12bTitle_120b55ec-3904-45de-8c11-5350f7470dbb" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_TradingSymbol_3ca4c836-d105-4645-8dd8-4810d83a1b0e" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_TradingSymbol"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_TradingSymbol_3ca4c836-d105-4645-8dd8-4810d83a1b0e" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_SecurityExchangeName_86d7a537-a065-48a0-8fdf-1dd90c6e441a" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_SecurityExchangeName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_SecurityExchangeName_86d7a537-a065-48a0-8fdf-1dd90c6e441a" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCurrentReportingStatus_f3eda2dd-a842-49ad-9a5d-23aa88e9436f" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityCurrentReportingStatus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_EntityCurrentReportingStatus_f3eda2dd-a842-49ad-9a5d-23aa88e9436f" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityInteractiveDataCurrent_39560267-7193-48a2-8a38-e22643841709" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityInteractiveDataCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_EntityInteractiveDataCurrent_39560267-7193-48a2-8a38-e22643841709" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFilerCategory_7fdc10f4-33bd-4100-bd36-da5b90b41899" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityFilerCategory"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_EntityFilerCategory_7fdc10f4-33bd-4100-bd36-da5b90b41899" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntitySmallBusiness_c6e648ef-2d75-4c35-9610-fec80ebb1b5b" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntitySmallBusiness"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_EntitySmallBusiness_c6e648ef-2d75-4c35-9610-fec80ebb1b5b" xlink:type="arc" order="21"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityEmergingGrowthCompany_aa508661-a463-4b1f-aac2-3a337ef0c436" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityEmergingGrowthCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_EntityEmergingGrowthCompany_aa508661-a463-4b1f-aac2-3a337ef0c436" xlink:type="arc" order="22"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityShellCompany_e1dacd1d-e8eb-4c05-81c2-92e83f611214" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityShellCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_EntityShellCompany_e1dacd1d-e8eb-4c05-81c2-92e83f611214" xlink:type="arc" order="23"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCommonStockSharesOutstanding_9e064d3d-5dad-4d0f-9e63-8af22a6e4884" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityCommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_EntityCommonStockSharesOutstanding_9e064d3d-5dad-4d0f-9e63-8af22a6e4884" xlink:type="arc" order="24"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CurrentFiscalYearEndDate_905580bd-7b56-4072-a8d6-d9c7768ae050" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_CurrentFiscalYearEndDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_CurrentFiscalYearEndDate_905580bd-7b56-4072-a8d6-d9c7768ae050" xlink:type="arc" order="25"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentFiscalYearFocus_c44edb95-260f-4632-8085-dca0e4bbb297" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_DocumentFiscalYearFocus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_DocumentFiscalYearFocus_c44edb95-260f-4632-8085-dca0e4bbb297" xlink:type="arc" order="26"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentFiscalPeriodFocus_300f2615-b358-4512-aeff-a39a9e98c558" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_DocumentFiscalPeriodFocus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_DocumentFiscalPeriodFocus_300f2615-b358-4512-aeff-a39a9e98c558" xlink:type="arc" order="27"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_AmendmentFlag_dfc5ef66-e83b-4169-b05b-0275a23dc96d" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_AmendmentFlag"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_32d7630a-7442-40e5-b795-05039631cad7" xlink:to="loc_dei_AmendmentFlag_dfc5ef66-e83b-4169-b05b-0275a23dc96d" xlink:type="arc" order="28"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/CondensedConsolidatedStatementsOfIncome" xlink:type="simple" xlink:href="lnt-20240331.xsd#CondensedConsolidatedStatementsOfIncome"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/CondensedConsolidatedStatementsOfIncome" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_72f1e106-6894-477b-90d8-2b43e6bf9c6d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_f0f41e7a-06fc-4cb3-8c87-1af90f452043" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_72f1e106-6894-477b-90d8-2b43e6bf9c6d" xlink:to="loc_us-gaap_StatementTable_f0f41e7a-06fc-4cb3-8c87-1af90f452043" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_572d24c7-7aa8-4996-a839-a001f5124aa6" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_f0f41e7a-06fc-4cb3-8c87-1af90f452043" xlink:to="loc_srt_ProductOrServiceAxis_572d24c7-7aa8-4996-a839-a001f5124aa6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_572d24c7-7aa8-4996-a839-a001f5124aa6_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_572d24c7-7aa8-4996-a839-a001f5124aa6" xlink:to="loc_srt_ProductsAndServicesDomain_572d24c7-7aa8-4996-a839-a001f5124aa6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_ba2ecabc-7b63-48d1-9e50-3943939fd3c8" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_572d24c7-7aa8-4996-a839-a001f5124aa6" xlink:to="loc_srt_ProductsAndServicesDomain_ba2ecabc-7b63-48d1-9e50-3943939fd3c8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_abe80dbe-24d1-455a-8664-21cf24e5efda" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_f0f41e7a-06fc-4cb3-8c87-1af90f452043" xlink:to="loc_dei_LegalEntityAxis_abe80dbe-24d1-455a-8664-21cf24e5efda" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_abe80dbe-24d1-455a-8664-21cf24e5efda_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_abe80dbe-24d1-455a-8664-21cf24e5efda" xlink:to="loc_dei_EntityDomain_abe80dbe-24d1-455a-8664-21cf24e5efda_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_9dd6d370-d7de-4e71-916d-e9c9a19ac864" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_abe80dbe-24d1-455a-8664-21cf24e5efda" xlink:to="loc_dei_EntityDomain_9dd6d370-d7de-4e71-916d-e9c9a19ac864" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_2d40f7a1-47f7-4072-b967-a0f7deeaf092" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_9dd6d370-d7de-4e71-916d-e9c9a19ac864" xlink:to="loc_lnt_IplMember_2d40f7a1-47f7-4072-b967-a0f7deeaf092" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_207e020b-b165-4fcd-b424-dcf6b3b4000f" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_9dd6d370-d7de-4e71-916d-e9c9a19ac864" xlink:to="loc_lnt_WplMember_207e020b-b165-4fcd-b424-dcf6b3b4000f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenuesAbstract_753f58dc-8a88-4d30-804e-14d76c80c8c8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RevenuesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_72f1e106-6894-477b-90d8-2b43e6bf9c6d" xlink:to="loc_us-gaap_RevenuesAbstract_753f58dc-8a88-4d30-804e-14d76c80c8c8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatedOperatingRevenueElectricNonNuclear_69e92246-0daa-4e8b-8823-5373fa329266" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatedOperatingRevenueElectricNonNuclear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenuesAbstract_753f58dc-8a88-4d30-804e-14d76c80c8c8" xlink:to="loc_us-gaap_RegulatedOperatingRevenueElectricNonNuclear_69e92246-0daa-4e8b-8823-5373fa329266" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatedOperatingRevenueGas_11cce512-cb8d-4610-9c31-98385de8388a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatedOperatingRevenueGas"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenuesAbstract_753f58dc-8a88-4d30-804e-14d76c80c8c8" xlink:to="loc_us-gaap_RegulatedOperatingRevenueGas_11cce512-cb8d-4610-9c31-98385de8388a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatedOperatingRevenueOther_a09def3c-5ab6-4b15-bc39-93b5f00e5470" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatedOperatingRevenueOther"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenuesAbstract_753f58dc-8a88-4d30-804e-14d76c80c8c8" xlink:to="loc_us-gaap_RegulatedOperatingRevenueOther_a09def3c-5ab6-4b15-bc39-93b5f00e5470" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnregulatedOperatingRevenue_90884998-1bb7-42ae-8377-0ac7b747c616" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_UnregulatedOperatingRevenue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenuesAbstract_753f58dc-8a88-4d30-804e-14d76c80c8c8" xlink:to="loc_us-gaap_UnregulatedOperatingRevenue_90884998-1bb7-42ae-8377-0ac7b747c616" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatedAndUnregulatedOperatingRevenue_80877b74-0b91-495e-850c-fc172fe55fed" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatedAndUnregulatedOperatingRevenue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenuesAbstract_753f58dc-8a88-4d30-804e-14d76c80c8c8" xlink:to="loc_us-gaap_RegulatedAndUnregulatedOperatingRevenue_80877b74-0b91-495e-850c-fc172fe55fed" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostsAndExpensesAbstract_cd359a3d-281b-4a95-87b9-a92088904119" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CostsAndExpensesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_72f1e106-6894-477b-90d8-2b43e6bf9c6d" xlink:to="loc_us-gaap_CostsAndExpensesAbstract_cd359a3d-281b-4a95-87b9-a92088904119" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UtilitiesOperatingExpenseFuelUsed_ca6e85cb-0875-4c62-b09c-0e048728edc2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_UtilitiesOperatingExpenseFuelUsed"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CostsAndExpensesAbstract_cd359a3d-281b-4a95-87b9-a92088904119" xlink:to="loc_us-gaap_UtilitiesOperatingExpenseFuelUsed_ca6e85cb-0875-4c62-b09c-0e048728edc2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfGoodsAndServicesSold_2ebf01af-1bf7-4a3e-b261-90aca8174246" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CostOfGoodsAndServicesSold"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CostsAndExpensesAbstract_cd359a3d-281b-4a95-87b9-a92088904119" xlink:to="loc_us-gaap_CostOfGoodsAndServicesSold_2ebf01af-1bf7-4a3e-b261-90aca8174246" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UtilitiesOperatingExpenseGasAndPetroleumPurchased_9e26c910-70b0-4a7c-b7f8-ac970355b1ac" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_UtilitiesOperatingExpenseGasAndPetroleumPurchased"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CostsAndExpensesAbstract_cd359a3d-281b-4a95-87b9-a92088904119" xlink:to="loc_us-gaap_UtilitiesOperatingExpenseGasAndPetroleumPurchased_9e26c910-70b0-4a7c-b7f8-ac970355b1ac" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherCostAndExpenseOperating_50547853-21c8-48a7-b053-3b2914da9efa" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherCostAndExpenseOperating"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CostsAndExpensesAbstract_cd359a3d-281b-4a95-87b9-a92088904119" xlink:to="loc_us-gaap_OtherCostAndExpenseOperating_50547853-21c8-48a7-b053-3b2914da9efa" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UtilitiesOperatingExpenseMaintenanceAndOperations_ff1716c4-eacc-45fc-a236-fa2de573fabe" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_UtilitiesOperatingExpenseMaintenanceAndOperations"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CostsAndExpensesAbstract_cd359a3d-281b-4a95-87b9-a92088904119" xlink:to="loc_us-gaap_UtilitiesOperatingExpenseMaintenanceAndOperations_ff1716c4-eacc-45fc-a236-fa2de573fabe" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepreciationAndAmortization_8d06640e-4993-481c-a940-5832524ba57c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DepreciationAndAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CostsAndExpensesAbstract_cd359a3d-281b-4a95-87b9-a92088904119" xlink:to="loc_us-gaap_DepreciationAndAmortization_8d06640e-4993-481c-a940-5832524ba57c" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TaxesExcludingIncomeAndExciseTaxes_d706e102-ad90-4a09-abdb-ad7d31a76767" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TaxesExcludingIncomeAndExciseTaxes"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CostsAndExpensesAbstract_cd359a3d-281b-4a95-87b9-a92088904119" xlink:to="loc_us-gaap_TaxesExcludingIncomeAndExciseTaxes_d706e102-ad90-4a09-abdb-ad7d31a76767" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostsAndExpenses_178b7a91-e50e-4424-88df-cf09707c4b88" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CostsAndExpenses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CostsAndExpensesAbstract_cd359a3d-281b-4a95-87b9-a92088904119" xlink:to="loc_us-gaap_CostsAndExpenses_178b7a91-e50e-4424-88df-cf09707c4b88" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_6e931c64-a673-4ca1-8978-a27fa77a138f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_72f1e106-6894-477b-90d8-2b43e6bf9c6d" xlink:to="loc_us-gaap_OperatingIncomeLoss_6e931c64-a673-4ca1-8978-a27fa77a138f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonoperatingIncomeExpenseAbstract_1d7d5c71-23ae-4c25-89bb-801998e35652" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NonoperatingIncomeExpenseAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_72f1e106-6894-477b-90d8-2b43e6bf9c6d" xlink:to="loc_us-gaap_NonoperatingIncomeExpenseAbstract_1d7d5c71-23ae-4c25-89bb-801998e35652" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpense_08d9ebfc-af9f-4dc7-a66c-59e86699ff48" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_InterestExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NonoperatingIncomeExpenseAbstract_1d7d5c71-23ae-4c25-89bb-801998e35652" xlink:to="loc_us-gaap_InterestExpense_08d9ebfc-af9f-4dc7-a66c-59e86699ff48" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromEquityMethodInvestments_9e746d20-ab00-4e57-8673-6a9aa6036510" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeLossFromEquityMethodInvestments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NonoperatingIncomeExpenseAbstract_1d7d5c71-23ae-4c25-89bb-801998e35652" xlink:to="loc_us-gaap_IncomeLossFromEquityMethodInvestments_9e746d20-ab00-4e57-8673-6a9aa6036510" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PublicUtilitiesAllowanceForFundsUsedDuringConstructionAdditions_63485172-c2c7-4c2e-85e5-6c75592c0f5f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PublicUtilitiesAllowanceForFundsUsedDuringConstructionAdditions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NonoperatingIncomeExpenseAbstract_1d7d5c71-23ae-4c25-89bb-801998e35652" xlink:to="loc_us-gaap_PublicUtilitiesAllowanceForFundsUsedDuringConstructionAdditions_63485172-c2c7-4c2e-85e5-6c75592c0f5f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNonoperatingIncomeExpense_d2b04e46-7a62-4e9f-b016-62a115d48bd4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherNonoperatingIncomeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NonoperatingIncomeExpenseAbstract_1d7d5c71-23ae-4c25-89bb-801998e35652" xlink:to="loc_us-gaap_OtherNonoperatingIncomeExpense_d2b04e46-7a62-4e9f-b016-62a115d48bd4" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonoperatingIncomeExpense_0aff72b0-9eb9-4492-80f5-cfb0f6cf5597" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NonoperatingIncomeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NonoperatingIncomeExpenseAbstract_1d7d5c71-23ae-4c25-89bb-801998e35652" xlink:to="loc_us-gaap_NonoperatingIncomeExpense_0aff72b0-9eb9-4492-80f5-cfb0f6cf5597" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_477ba75e-f2cd-48a0-84f9-476449bb7867" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_72f1e106-6894-477b-90d8-2b43e6bf9c6d" xlink:to="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_477ba75e-f2cd-48a0-84f9-476449bb7867" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_b06f929a-3c47-4d3d-bfa6-914516737f26" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeTaxExpenseBenefit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_72f1e106-6894-477b-90d8-2b43e6bf9c6d" xlink:to="loc_us-gaap_IncomeTaxExpenseBenefit_b06f929a-3c47-4d3d-bfa6-914516737f26" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_f591ca4c-c5db-44be-b334-87c7ca210fdb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_72f1e106-6894-477b-90d8-2b43e6bf9c6d" xlink:to="loc_us-gaap_NetIncomeLoss_f591ca4c-c5db-44be-b334-87c7ca210fdb" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic_17addf44-0648-4ad4-b50a-30aac7697a89" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_72f1e106-6894-477b-90d8-2b43e6bf9c6d" xlink:to="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic_17addf44-0648-4ad4-b50a-30aac7697a89" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasicAbstract_11eb0f2a-ec96-40ff-8a57-0579178c572e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_WeightedAverageNumberOfSharesOutstandingBasicAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_72f1e106-6894-477b-90d8-2b43e6bf9c6d" xlink:to="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasicAbstract_11eb0f2a-ec96-40ff-8a57-0579178c572e" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_39feba02-505e-46dd-a680-d52142c5fc20" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_WeightedAverageNumberOfSharesOutstandingBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasicAbstract_11eb0f2a-ec96-40ff-8a57-0579178c572e" xlink:to="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_39feba02-505e-46dd-a680-d52142c5fc20" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_6a2ff365-4558-4281-abf0-6e8f5b1e16e3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasicAbstract_11eb0f2a-ec96-40ff-8a57-0579178c572e" xlink:to="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_6a2ff365-4558-4281-abf0-6e8f5b1e16e3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareAbstract_75186786-9ffd-48bd-953d-e4fe0a1ea10d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EarningsPerShareAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_72f1e106-6894-477b-90d8-2b43e6bf9c6d" xlink:to="loc_us-gaap_EarningsPerShareAbstract_75186786-9ffd-48bd-953d-e4fe0a1ea10d" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareBasic_d04d4ac1-da8d-44c1-8b8f-bdba69e66e9a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EarningsPerShareBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EarningsPerShareAbstract_75186786-9ffd-48bd-953d-e4fe0a1ea10d" xlink:to="loc_us-gaap_EarningsPerShareBasic_d04d4ac1-da8d-44c1-8b8f-bdba69e66e9a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareDiluted_42965b88-fc2e-410f-b610-c11e464dff73" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EarningsPerShareDiluted"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EarningsPerShareAbstract_75186786-9ffd-48bd-953d-e4fe0a1ea10d" xlink:to="loc_us-gaap_EarningsPerShareDiluted_42965b88-fc2e-410f-b610-c11e464dff73" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/CondensedConsolidatedBalanceSheets" xlink:type="simple" xlink:href="lnt-20240331.xsd#CondensedConsolidatedBalanceSheets"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/CondensedConsolidatedBalanceSheets" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_d9397c84-69ec-4ce6-94e5-d9be2d3de2b2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_9b87262c-206b-4766-ac99-23551c78d2cb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_d9397c84-69ec-4ce6-94e5-d9be2d3de2b2" xlink:to="loc_us-gaap_StatementTable_9b87262c-206b-4766-ac99-23551c78d2cb" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_ae686205-184c-428f-ae69-0aec92012ee1" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_9b87262c-206b-4766-ac99-23551c78d2cb" xlink:to="loc_dei_LegalEntityAxis_ae686205-184c-428f-ae69-0aec92012ee1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_ae686205-184c-428f-ae69-0aec92012ee1_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_ae686205-184c-428f-ae69-0aec92012ee1" xlink:to="loc_dei_EntityDomain_ae686205-184c-428f-ae69-0aec92012ee1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_33b8aac0-a941-4cf1-abee-7ffb70f95d09" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_ae686205-184c-428f-ae69-0aec92012ee1" xlink:to="loc_dei_EntityDomain_33b8aac0-a941-4cf1-abee-7ffb70f95d09" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_71641407-9a76-44e5-85ec-420e6154e658" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_33b8aac0-a941-4cf1-abee-7ffb70f95d09" xlink:to="loc_lnt_IplMember_71641407-9a76-44e5-85ec-420e6154e658" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_0e6692be-98a9-4cba-b520-bfdf34036caf" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_33b8aac0-a941-4cf1-abee-7ffb70f95d09" xlink:to="loc_lnt_WplMember_0e6692be-98a9-4cba-b520-bfdf34036caf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsAbstract_ff346816-77aa-45c1-aac2-7223ba032f85" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AssetsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_d9397c84-69ec-4ce6-94e5-d9be2d3de2b2" xlink:to="loc_us-gaap_AssetsAbstract_ff346816-77aa-45c1-aac2-7223ba032f85" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrentAbstract_96109d28-4d9b-46a8-b997-4ebd8a05fec6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AssetsCurrentAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_ff346816-77aa-45c1-aac2-7223ba032f85" xlink:to="loc_us-gaap_AssetsCurrentAbstract_96109d28-4d9b-46a8-b997-4ebd8a05fec6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_fff79760-5eb0-48d9-a78f-8c6e5c775417" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_96109d28-4d9b-46a8-b997-4ebd8a05fec6" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_fff79760-5eb0-48d9-a78f-8c6e5c775417" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsReceivableNetCurrent_5839eede-d18d-4e20-8b5f-28e6ae8c4358" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccountsReceivableNetCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_96109d28-4d9b-46a8-b997-4ebd8a05fec6" xlink:to="loc_us-gaap_AccountsReceivableNetCurrent_5839eede-d18d-4e20-8b5f-28e6ae8c4358" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EnergyRelatedInventoryOtherFossilFuel_d7b9368e-b1e2-4899-9beb-7b15ded938a7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EnergyRelatedInventoryOtherFossilFuel"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_96109d28-4d9b-46a8-b997-4ebd8a05fec6" xlink:to="loc_us-gaap_EnergyRelatedInventoryOtherFossilFuel_d7b9368e-b1e2-4899-9beb-7b15ded938a7" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EnergyRelatedInventoryGasStoredUnderground_497eca9c-c196-444f-8dea-ea9029834732" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EnergyRelatedInventoryGasStoredUnderground"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_96109d28-4d9b-46a8-b997-4ebd8a05fec6" xlink:to="loc_us-gaap_EnergyRelatedInventoryGasStoredUnderground_497eca9c-c196-444f-8dea-ea9029834732" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryRawMaterialsAndSupplies_62fb0fa9-26c8-492d-b092-93ddae06e714" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_InventoryRawMaterialsAndSupplies"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_96109d28-4d9b-46a8-b997-4ebd8a05fec6" xlink:to="loc_us-gaap_InventoryRawMaterialsAndSupplies_62fb0fa9-26c8-492d-b092-93ddae06e714" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryAssetsCurrent_ca703f6f-e4c2-46d0-89f7-62ece74d2045" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatoryAssetsCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_96109d28-4d9b-46a8-b997-4ebd8a05fec6" xlink:to="loc_us-gaap_RegulatoryAssetsCurrent_ca703f6f-e4c2-46d0-89f7-62ece74d2045" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrepaidTaxes_d256d94b-bf64-4e2d-9190-a7a7252d39cc" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PrepaidTaxes"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_96109d28-4d9b-46a8-b997-4ebd8a05fec6" xlink:to="loc_us-gaap_PrepaidTaxes_d256d94b-bf64-4e2d-9190-a7a7252d39cc" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsCurrent_14b12cfc-972d-4866-bb36-f28016febc94" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherAssetsCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_96109d28-4d9b-46a8-b997-4ebd8a05fec6" xlink:to="loc_us-gaap_OtherAssetsCurrent_14b12cfc-972d-4866-bb36-f28016febc94" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_9d4de941-252e-420b-81ac-d8e31d08ba6e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AssetsCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsCurrentAbstract_96109d28-4d9b-46a8-b997-4ebd8a05fec6" xlink:to="loc_us-gaap_AssetsCurrent_9d4de941-252e-420b-81ac-d8e31d08ba6e" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_9879ff51-6b18-40be-81a8-822df401d120" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_ff346816-77aa-45c1-aac2-7223ba032f85" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_9879ff51-6b18-40be-81a8-822df401d120" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermInvestmentsAbstract_2a06e038-f1dc-45f2-9801-2b0ba77aa4dc" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermInvestmentsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_ff346816-77aa-45c1-aac2-7223ba032f85" xlink:to="loc_us-gaap_LongTermInvestmentsAbstract_2a06e038-f1dc-45f2-9801-2b0ba77aa4dc" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_InvestmentsBusinessLine1_02c9a026-429c-47f3-a475-7cc586319dc5" xlink:href="lnt-20240331.xsd#lnt_InvestmentsBusinessLine1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermInvestmentsAbstract_2a06e038-f1dc-45f2-9801-2b0ba77aa4dc" xlink:to="loc_lnt_InvestmentsBusinessLine1_02c9a026-429c-47f3-a475-7cc586319dc5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLongTermInvestments_52c99e88-f6f1-4018-84cf-7fc79d815007" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherLongTermInvestments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermInvestmentsAbstract_2a06e038-f1dc-45f2-9801-2b0ba77aa4dc" xlink:to="loc_us-gaap_OtherLongTermInvestments_52c99e88-f6f1-4018-84cf-7fc79d815007" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermInvestments_cca4c437-4017-413b-a5b0-e3d42acea52a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermInvestments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermInvestmentsAbstract_2a06e038-f1dc-45f2-9801-2b0ba77aa4dc" xlink:to="loc_us-gaap_LongTermInvestments_cca4c437-4017-413b-a5b0-e3d42acea52a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsNoncurrentAbstract_88d8b9a0-5a7f-4700-bc29-6622658058bf" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherAssetsNoncurrentAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_ff346816-77aa-45c1-aac2-7223ba032f85" xlink:to="loc_us-gaap_OtherAssetsNoncurrentAbstract_88d8b9a0-5a7f-4700-bc29-6622658058bf" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryAssetsNoncurrent_b8bf0b89-074d-4d36-929a-46b67b5f848d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatoryAssetsNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherAssetsNoncurrentAbstract_88d8b9a0-5a7f-4700-bc29-6622658058bf" xlink:to="loc_us-gaap_RegulatoryAssetsNoncurrent_b8bf0b89-074d-4d36-929a-46b67b5f848d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredCosts_1181b83b-b576-4b3b-97b3-fe33340279df" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DeferredCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherAssetsNoncurrentAbstract_88d8b9a0-5a7f-4700-bc29-6622658058bf" xlink:to="loc_us-gaap_DeferredCosts_1181b83b-b576-4b3b-97b3-fe33340279df" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsNoncurrent_9d8df65e-0334-4d73-bbdc-88da02414b8d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherAssetsNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherAssetsNoncurrentAbstract_88d8b9a0-5a7f-4700-bc29-6622658058bf" xlink:to="loc_us-gaap_OtherAssetsNoncurrent_9d8df65e-0334-4d73-bbdc-88da02414b8d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Assets_a3d481eb-c0fe-48b4-b94e-bb01be9ac3c3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_Assets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_d9397c84-69ec-4ce6-94e5-d9be2d3de2b2" xlink:to="loc_us-gaap_Assets_a3d481eb-c0fe-48b4-b94e-bb01be9ac3c3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_f2ef569a-e0d3-4552-bc76-09ed29f47b6e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LiabilitiesAndStockholdersEquityAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_d9397c84-69ec-4ce6-94e5-d9be2d3de2b2" xlink:to="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_f2ef569a-e0d3-4552-bc76-09ed29f47b6e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrentAbstract_7b3c4964-ca01-45c0-bc55-49b67a7ea425" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LiabilitiesCurrentAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_f2ef569a-e0d3-4552-bc76-09ed29f47b6e" xlink:to="loc_us-gaap_LiabilitiesCurrentAbstract_7b3c4964-ca01-45c0-bc55-49b67a7ea425" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtCurrent_7f3adde7-4241-4c14-b84d-2c331334f026" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermDebtCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_7b3c4964-ca01-45c0-bc55-49b67a7ea425" xlink:to="loc_us-gaap_LongTermDebtCurrent_7f3adde7-4241-4c14-b84d-2c331334f026" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialPaper_5e235925-5fc0-48aa-af1e-228bcf7a354f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommercialPaper"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_7b3c4964-ca01-45c0-bc55-49b67a7ea425" xlink:to="loc_us-gaap_CommercialPaper_5e235925-5fc0-48aa-af1e-228bcf7a354f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsPayableCurrent_8b8ecf72-4b0c-47c8-9fc7-1118b2ee8de8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccountsPayableCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_7b3c4964-ca01-45c0-bc55-49b67a7ea425" xlink:to="loc_us-gaap_AccountsPayableCurrent_8b8ecf72-4b0c-47c8-9fc7-1118b2ee8de8" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TaxesPayableCurrent_92c1f2fb-6fad-4732-ba06-e0b59ba8ef46" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TaxesPayableCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_7b3c4964-ca01-45c0-bc55-49b67a7ea425" xlink:to="loc_us-gaap_TaxesPayableCurrent_92c1f2fb-6fad-4732-ba06-e0b59ba8ef46" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestPayableCurrent_96026692-2c82-44b7-b746-df9c8f6bddde" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_InterestPayableCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_7b3c4964-ca01-45c0-bc55-49b67a7ea425" xlink:to="loc_us-gaap_InterestPayableCurrent_96026692-2c82-44b7-b746-df9c8f6bddde" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryLiabilityCurrent_022663df-ba0c-41c0-9625-90ba58290124" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatoryLiabilityCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_7b3c4964-ca01-45c0-bc55-49b67a7ea425" xlink:to="loc_us-gaap_RegulatoryLiabilityCurrent_022663df-ba0c-41c0-9625-90ba58290124" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilitiesCurrent_e6919d41-8532-4c29-8963-4be76452db04" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherLiabilitiesCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_7b3c4964-ca01-45c0-bc55-49b67a7ea425" xlink:to="loc_us-gaap_OtherLiabilitiesCurrent_e6919d41-8532-4c29-8963-4be76452db04" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_3407ae3c-a76c-49d3-b610-74f18c279054" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LiabilitiesCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesCurrentAbstract_7b3c4964-ca01-45c0-bc55-49b67a7ea425" xlink:to="loc_us-gaap_LiabilitiesCurrent_3407ae3c-a76c-49d3-b610-74f18c279054" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtNoncurrent_f3a5ecc0-f5d9-400e-8a01-8a43e1db5f32" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermDebtNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_f2ef569a-e0d3-4552-bc76-09ed29f47b6e" xlink:to="loc_us-gaap_LongTermDebtNoncurrent_f3a5ecc0-f5d9-400e-8a01-8a43e1db5f32" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredCreditsAndOtherLiabilitiesAbstract_9bbabe8d-3483-45b4-a3cc-9db3d0aa14ee" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DeferredCreditsAndOtherLiabilitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_f2ef569a-e0d3-4552-bc76-09ed29f47b6e" xlink:to="loc_us-gaap_DeferredCreditsAndOtherLiabilitiesAbstract_9bbabe8d-3483-45b4-a3cc-9db3d0aa14ee" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredIncomeTaxLiabilitiesNet_aa20f120-064d-4dc1-b266-a97faaaa1ac1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DeferredIncomeTaxLiabilitiesNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DeferredCreditsAndOtherLiabilitiesAbstract_9bbabe8d-3483-45b4-a3cc-9db3d0aa14ee" xlink:to="loc_us-gaap_DeferredIncomeTaxLiabilitiesNet_aa20f120-064d-4dc1-b266-a97faaaa1ac1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryLiabilityNoncurrent_dd50e6d8-ea6f-4183-8f16-0c411486cee2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatoryLiabilityNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DeferredCreditsAndOtherLiabilitiesAbstract_9bbabe8d-3483-45b4-a3cc-9db3d0aa14ee" xlink:to="loc_us-gaap_RegulatoryLiabilityNoncurrent_dd50e6d8-ea6f-4183-8f16-0c411486cee2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PensionAndOtherPostretirementDefinedBenefitPlansLiabilitiesNoncurrent_a9e8b94c-e5ac-47c0-a569-c68f7efa89b5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PensionAndOtherPostretirementDefinedBenefitPlansLiabilitiesNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DeferredCreditsAndOtherLiabilitiesAbstract_9bbabe8d-3483-45b4-a3cc-9db3d0aa14ee" xlink:to="loc_us-gaap_PensionAndOtherPostretirementDefinedBenefitPlansLiabilitiesNoncurrent_a9e8b94c-e5ac-47c0-a569-c68f7efa89b5" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilitiesNoncurrent_985ce5d6-3925-4a04-9ff2-4e3bfe9c3e2a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherLiabilitiesNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DeferredCreditsAndOtherLiabilitiesAbstract_9bbabe8d-3483-45b4-a3cc-9db3d0aa14ee" xlink:to="loc_us-gaap_OtherLiabilitiesNoncurrent_985ce5d6-3925-4a04-9ff2-4e3bfe9c3e2a" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredCreditsAndOtherLiabilities_a1e84f7b-3d98-4610-9954-f4de1b71e5e1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DeferredCreditsAndOtherLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DeferredCreditsAndOtherLiabilitiesAbstract_9bbabe8d-3483-45b4-a3cc-9db3d0aa14ee" xlink:to="loc_us-gaap_DeferredCreditsAndOtherLiabilities_a1e84f7b-3d98-4610-9954-f4de1b71e5e1" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommitmentsAndContingencies_5b7098e2-3d45-4569-b7fc-f8483db4b356" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommitmentsAndContingencies"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_f2ef569a-e0d3-4552-bc76-09ed29f47b6e" xlink:to="loc_us-gaap_CommitmentsAndContingencies_5b7098e2-3d45-4569-b7fc-f8483db4b356" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CapitalizationLongtermDebtAndEquityAbstract_6e8a8199-0f66-49dd-be20-c996af38fe39" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CapitalizationLongtermDebtAndEquityAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_f2ef569a-e0d3-4552-bc76-09ed29f47b6e" xlink:to="loc_us-gaap_CapitalizationLongtermDebtAndEquityAbstract_6e8a8199-0f66-49dd-be20-c996af38fe39" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityAbstract_bedf023a-0efc-4bd9-ad70-65f68c370720" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockholdersEquityAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CapitalizationLongtermDebtAndEquityAbstract_6e8a8199-0f66-49dd-be20-c996af38fe39" xlink:to="loc_us-gaap_StockholdersEquityAbstract_bedf023a-0efc-4bd9-ad70-65f68c370720" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockValue_ed490308-b817-4ca2-a40b-650e47621c7a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_bedf023a-0efc-4bd9-ad70-65f68c370720" xlink:to="loc_us-gaap_CommonStockValue_ed490308-b817-4ca2-a40b-650e47621c7a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalCommonStock_1fa8014e-1350-473f-bbd6-53bd49335147" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AdditionalPaidInCapitalCommonStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_bedf023a-0efc-4bd9-ad70-65f68c370720" xlink:to="loc_us-gaap_AdditionalPaidInCapitalCommonStock_1fa8014e-1350-473f-bbd6-53bd49335147" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsAccumulatedDeficit_497c5355-90b5-4f9e-b598-5b4fdb378dab" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RetainedEarningsAccumulatedDeficit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_bedf023a-0efc-4bd9-ad70-65f68c370720" xlink:to="loc_us-gaap_RetainedEarningsAccumulatedDeficit_497c5355-90b5-4f9e-b598-5b4fdb378dab" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_9b977fe1-bfe2-4997-915d-6a52b4688925" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_bedf023a-0efc-4bd9-ad70-65f68c370720" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_9b977fe1-bfe2-4997-915d-6a52b4688925" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockHeldInTrust_f8fb16d6-4e4a-4c8b-823d-1fa01ae7d768" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockHeldInTrust"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_bedf023a-0efc-4bd9-ad70-65f68c370720" xlink:to="loc_us-gaap_CommonStockHeldInTrust_f8fb16d6-4e4a-4c8b-823d-1fa01ae7d768" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_03298f85-c489-4224-a3ba-f643257debc9" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CapitalizationLongtermDebtAndEquityAbstract_6e8a8199-0f66-49dd-be20-c996af38fe39" xlink:to="loc_us-gaap_StockholdersEquity_03298f85-c489-4224-a3ba-f643257debc9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquity_6f7f469f-5654-4edd-b1ce-684cb45ec155" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LiabilitiesAndStockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_d9397c84-69ec-4ce6-94e5-d9be2d3de2b2" xlink:to="loc_us-gaap_LiabilitiesAndStockholdersEquity_6f7f469f-5654-4edd-b1ce-684cb45ec155" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/CondensedConsolidatedBalanceSheetsParenthetical" xlink:type="simple" xlink:href="lnt-20240331.xsd#CondensedConsolidatedBalanceSheetsParenthetical"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/CondensedConsolidatedBalanceSheetsParenthetical" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_b7f7ac02-7e24-4e02-85ea-a649e4d77382" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_aab516b8-5641-4d17-bfd4-db2d5c91e3e0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_b7f7ac02-7e24-4e02-85ea-a649e4d77382" xlink:to="loc_us-gaap_StatementTable_aab516b8-5641-4d17-bfd4-db2d5c91e3e0" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_64694edf-38d6-4759-9a5f-f7fc7dea4a60" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_aab516b8-5641-4d17-bfd4-db2d5c91e3e0" xlink:to="loc_dei_LegalEntityAxis_64694edf-38d6-4759-9a5f-f7fc7dea4a60" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_64694edf-38d6-4759-9a5f-f7fc7dea4a60_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_64694edf-38d6-4759-9a5f-f7fc7dea4a60" xlink:to="loc_dei_EntityDomain_64694edf-38d6-4759-9a5f-f7fc7dea4a60_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_2a3c1da4-4952-41a4-ac68-6c3e5ed584e8" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_64694edf-38d6-4759-9a5f-f7fc7dea4a60" xlink:to="loc_dei_EntityDomain_2a3c1da4-4952-41a4-ac68-6c3e5ed584e8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_54156c19-7ce1-4df2-a0f8-6008c9095341" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_2a3c1da4-4952-41a4-ac68-6c3e5ed584e8" xlink:to="loc_lnt_IplMember_54156c19-7ce1-4df2-a0f8-6008c9095341" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_35e0a802-8306-4abd-ab4c-ae36f215128f" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_2a3c1da4-4952-41a4-ac68-6c3e5ed584e8" xlink:to="loc_lnt_WplMember_35e0a802-8306-4abd-ab4c-ae36f215128f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockParOrStatedValuePerShare_ab4aafab-dcf4-46ac-8faf-0463a594c0a7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockParOrStatedValuePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_b7f7ac02-7e24-4e02-85ea-a649e4d77382" xlink:to="loc_us-gaap_CommonStockParOrStatedValuePerShare_ab4aafab-dcf4-46ac-8faf-0463a594c0a7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesAuthorized_79a642d9-1870-45e6-aa85-6b7e0760c838" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_b7f7ac02-7e24-4e02-85ea-a649e4d77382" xlink:to="loc_us-gaap_CommonStockSharesAuthorized_79a642d9-1870-45e6-aa85-6b7e0760c838" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_4bd0ef1e-c233-4f15-899b-f0caed76296d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_b7f7ac02-7e24-4e02-85ea-a649e4d77382" xlink:to="loc_us-gaap_CommonStockSharesOutstanding_4bd0ef1e-c233-4f15-899b-f0caed76296d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_CommonStockHeldInTrustShares_65121ad1-ed1f-418d-a5a4-39e7f6ee122f" xlink:href="lnt-20240331.xsd#lnt_CommonStockHeldInTrustShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_b7f7ac02-7e24-4e02-85ea-a649e4d77382" xlink:to="loc_lnt_CommonStockHeldInTrustShares_65121ad1-ed1f-418d-a5a4-39e7f6ee122f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardPerShareWeightedAveragePriceOfSharesPurchased_b7e194c4-1e8f-4734-b474-7f027b871eac" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardPerShareWeightedAveragePriceOfSharesPurchased"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_b7f7ac02-7e24-4e02-85ea-a649e4d77382" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardPerShareWeightedAveragePriceOfSharesPurchased_b7e194c4-1e8f-4734-b474-7f027b871eac" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/CondensedConsolidatedStatementsOfCashFlows" xlink:type="simple" xlink:href="lnt-20240331.xsd#CondensedConsolidatedStatementsOfCashFlows"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/CondensedConsolidatedStatementsOfCashFlows" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_cb1cfce9-fe5b-46c4-8af3-08d437dfded3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_76f89ef9-f401-4836-9ce6-044f34b2d042" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_cb1cfce9-fe5b-46c4-8af3-08d437dfded3" xlink:to="loc_us-gaap_StatementTable_76f89ef9-f401-4836-9ce6-044f34b2d042" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_5275d7cf-00c1-48f6-a8f2-2b7436f1c1cf" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_76f89ef9-f401-4836-9ce6-044f34b2d042" xlink:to="loc_dei_LegalEntityAxis_5275d7cf-00c1-48f6-a8f2-2b7436f1c1cf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_5275d7cf-00c1-48f6-a8f2-2b7436f1c1cf_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_5275d7cf-00c1-48f6-a8f2-2b7436f1c1cf" xlink:to="loc_dei_EntityDomain_5275d7cf-00c1-48f6-a8f2-2b7436f1c1cf_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_d0ccdbb5-fab7-4c46-a8e4-4f57bd93a755" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_5275d7cf-00c1-48f6-a8f2-2b7436f1c1cf" xlink:to="loc_dei_EntityDomain_d0ccdbb5-fab7-4c46-a8e4-4f57bd93a755" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_d923abd3-88fc-4eb4-b239-674e3c2a7cf1" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_d0ccdbb5-fab7-4c46-a8e4-4f57bd93a755" xlink:to="loc_lnt_IplMember_d923abd3-88fc-4eb4-b239-674e3c2a7cf1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_34a06a14-957e-4b14-b677-c98c7613b6fc" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_d0ccdbb5-fab7-4c46-a8e4-4f57bd93a755" xlink:to="loc_lnt_WplMember_34a06a14-957e-4b14-b677-c98c7613b6fc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_c7aa3977-c1e0-4331-87d9-3ab483cc3f94" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cb1cfce9-fe5b-46c4-8af3-08d437dfded3" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_c7aa3977-c1e0-4331-87d9-3ab483cc3f94" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_07433a24-4a50-4a52-82b8-17fe8f9cfe26" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_c7aa3977-c1e0-4331-87d9-3ab483cc3f94" xlink:to="loc_us-gaap_NetIncomeLoss_07433a24-4a50-4a52-82b8-17fe8f9cfe26" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_c54adf33-31b2-44d9-87d2-4fcf1613847b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_c7aa3977-c1e0-4331-87d9-3ab483cc3f94" xlink:to="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_c54adf33-31b2-44d9-87d2-4fcf1613847b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepreciationDepletionAndAmortization_e8a165ff-75c0-42da-92ee-82d664d0addb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DepreciationDepletionAndAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_c54adf33-31b2-44d9-87d2-4fcf1613847b" xlink:to="loc_us-gaap_DepreciationDepletionAndAmortization_e8a165ff-75c0-42da-92ee-82d664d0addb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNoncashIncomeExpense_175ae97a-7199-4178-9450-a534aff23b17" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherNoncashIncomeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_c54adf33-31b2-44d9-87d2-4fcf1613847b" xlink:to="loc_us-gaap_OtherNoncashIncomeExpense_175ae97a-7199-4178-9450-a534aff23b17" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_b18d7470-e298-4372-b017-b5b86022b265" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncreaseDecreaseInOperatingCapitalAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_c7aa3977-c1e0-4331-87d9-3ab483cc3f94" xlink:to="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_b18d7470-e298-4372-b017-b5b86022b265" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsReceivable_9433cb47-0084-473d-9737-aef60cc5f5b1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncreaseDecreaseInAccountsReceivable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_b18d7470-e298-4372-b017-b5b86022b265" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsReceivable_9433cb47-0084-473d-9737-aef60cc5f5b1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInFuelInventories_b243fdcf-cf63-4ff2-9337-e669182dfefb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncreaseDecreaseInFuelInventories"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_b18d7470-e298-4372-b017-b5b86022b265" xlink:to="loc_us-gaap_IncreaseDecreaseInFuelInventories_b243fdcf-cf63-4ff2-9337-e669182dfefb" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInDerivativeAssets_386e9c47-6a54-4971-9cfc-d3acc7688d22" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncreaseDecreaseInDerivativeAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_b18d7470-e298-4372-b017-b5b86022b265" xlink:to="loc_us-gaap_IncreaseDecreaseInDerivativeAssets_386e9c47-6a54-4971-9cfc-d3acc7688d22" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInOtherRegulatoryAssets_1877b347-9725-4736-8820-a1364eea2a0c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncreaseDecreaseInOtherRegulatoryAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_b18d7470-e298-4372-b017-b5b86022b265" xlink:to="loc_us-gaap_IncreaseDecreaseInOtherRegulatoryAssets_1877b347-9725-4736-8820-a1364eea2a0c" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsPayable_127af8bd-fdf5-41a1-94bd-1c03f40005a7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncreaseDecreaseInAccountsPayable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_b18d7470-e298-4372-b017-b5b86022b265" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsPayable_127af8bd-fdf5-41a1-94bd-1c03f40005a7" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInDerivativeLiabilities_1290b76a-692a-49f5-83dc-b9e12cfceef6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncreaseDecreaseInDerivativeLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_b18d7470-e298-4372-b017-b5b86022b265" xlink:to="loc_us-gaap_IncreaseDecreaseInDerivativeLiabilities_1290b76a-692a-49f5-83dc-b9e12cfceef6" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInRegulatoryLiabilities_6a41bb41-bd11-493c-b3cb-13afe5004322" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncreaseDecreaseInRegulatoryLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_b18d7470-e298-4372-b017-b5b86022b265" xlink:to="loc_us-gaap_IncreaseDecreaseInRegulatoryLiabilities_6a41bb41-bd11-493c-b3cb-13afe5004322" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInOtherOperatingCapitalNet_f0b0fe27-280c-4be9-8162-f4347fbb4a96" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncreaseDecreaseInOtherOperatingCapitalNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_b18d7470-e298-4372-b017-b5b86022b265" xlink:to="loc_us-gaap_IncreaseDecreaseInOtherOperatingCapitalNet_f0b0fe27-280c-4be9-8162-f4347fbb4a96" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_3e49ef82-0304-4cb6-8781-3c53428ace8d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_c7aa3977-c1e0-4331-87d9-3ab483cc3f94" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_3e49ef82-0304-4cb6-8781-3c53428ace8d" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_17cac67c-cdb2-4a97-882e-62efa7ca721c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cb1cfce9-fe5b-46c4-8af3-08d437dfded3" xlink:to="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_17cac67c-cdb2-4a97-882e-62efa7ca721c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_31a2892c-7a53-4499-933f-6832d9103655" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PaymentsToAcquirePropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_17cac67c-cdb2-4a97-882e-62efa7ca721c" xlink:to="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_31a2892c-7a53-4499-933f-6832d9103655" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireOtherProductiveAssets_69619bd6-b99b-4f44-965e-91933caad983" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PaymentsToAcquireOtherProductiveAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_17cac67c-cdb2-4a97-882e-62efa7ca721c" xlink:to="loc_us-gaap_PaymentsToAcquireOtherProductiveAssets_69619bd6-b99b-4f44-965e-91933caad983" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromCollectionOfRetainedInterestInSecuritizedReceivables_95265109-f314-4257-9d98-83c634b3a29f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ProceedsFromCollectionOfRetainedInterestInSecuritizedReceivables"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_17cac67c-cdb2-4a97-882e-62efa7ca721c" xlink:to="loc_us-gaap_ProceedsFromCollectionOfRetainedInterestInSecuritizedReceivables_95265109-f314-4257-9d98-83c634b3a29f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromSalesOfAssetsInvestingActivities_790082ae-bd61-48b1-ada5-a4159ef5341a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ProceedsFromSalesOfAssetsInvestingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_17cac67c-cdb2-4a97-882e-62efa7ca721c" xlink:to="loc_us-gaap_ProceedsFromSalesOfAssetsInvestingActivities_790082ae-bd61-48b1-ada5-a4159ef5341a" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsForProceedsFromOtherInvestingActivities_736ff577-adb6-4cd9-ad37-f9eb3c936abd" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PaymentsForProceedsFromOtherInvestingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_17cac67c-cdb2-4a97-882e-62efa7ca721c" xlink:to="loc_us-gaap_PaymentsForProceedsFromOtherInvestingActivities_736ff577-adb6-4cd9-ad37-f9eb3c936abd" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_82fac090-a4f5-4995-9869-234a735e142a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_17cac67c-cdb2-4a97-882e-62efa7ca721c" xlink:to="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_82fac090-a4f5-4995-9869-234a735e142a" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_d16bd2af-f549-43f0-a6c5-043ed9a18f6f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cb1cfce9-fe5b-46c4-8af3-08d437dfded3" xlink:to="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_d16bd2af-f549-43f0-a6c5-043ed9a18f6f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsOfDividendsCommonStock_3eedc59a-8cd5-41ff-bae3-c073a35563a4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PaymentsOfDividendsCommonStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_d16bd2af-f549-43f0-a6c5-043ed9a18f6f" xlink:to="loc_us-gaap_PaymentsOfDividendsCommonStock_3eedc59a-8cd5-41ff-bae3-c073a35563a4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromContributionsFromParent_668c3ca3-85e6-4ff8-8f03-bca308890b68" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ProceedsFromContributionsFromParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_d16bd2af-f549-43f0-a6c5-043ed9a18f6f" xlink:to="loc_us-gaap_ProceedsFromContributionsFromParent_668c3ca3-85e6-4ff8-8f03-bca308890b68" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOfLongTermDebt_84d47663-6925-4962-b882-acdc8cd21750" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ProceedsFromIssuanceOfLongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_d16bd2af-f549-43f0-a6c5-043ed9a18f6f" xlink:to="loc_us-gaap_ProceedsFromIssuanceOfLongTermDebt_84d47663-6925-4962-b882-acdc8cd21750" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RepaymentsOfLongTermDebt_3b097e1e-4dd4-4964-82c8-052313d7168b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RepaymentsOfLongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_d16bd2af-f549-43f0-a6c5-043ed9a18f6f" xlink:to="loc_us-gaap_RepaymentsOfLongTermDebt_3b097e1e-4dd4-4964-82c8-052313d7168b" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromRepaymentsOfShortTermDebt_bb3b65e0-52ed-468a-94f1-31b7bd520f7e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ProceedsFromRepaymentsOfShortTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_d16bd2af-f549-43f0-a6c5-043ed9a18f6f" xlink:to="loc_us-gaap_ProceedsFromRepaymentsOfShortTermDebt_bb3b65e0-52ed-468a-94f1-31b7bd520f7e" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromPaymentsForOtherFinancingActivities_21542de9-600b-4c86-9c7e-b66ab7d9b3c8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ProceedsFromPaymentsForOtherFinancingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_d16bd2af-f549-43f0-a6c5-043ed9a18f6f" xlink:to="loc_us-gaap_ProceedsFromPaymentsForOtherFinancingActivities_21542de9-600b-4c86-9c7e-b66ab7d9b3c8" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_cb6fac01-b2f9-42ba-94a6-05ab8ff4a567" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_d16bd2af-f549-43f0-a6c5-043ed9a18f6f" xlink:to="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_cb6fac01-b2f9-42ba-94a6-05ab8ff4a567" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_3ce8a48e-ef6e-4284-a817-5dafd0590273" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cb1cfce9-fe5b-46c4-8af3-08d437dfded3" xlink:to="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_3ce8a48e-ef6e-4284-a817-5dafd0590273" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsIncludingDisposalGroupAndDiscontinuedOperations_219886a0-b701-4ca6-b0ac-2971c7eaa9e7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsIncludingDisposalGroupAndDiscontinuedOperations"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cb1cfce9-fe5b-46c4-8af3-08d437dfded3" xlink:to="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsIncludingDisposalGroupAndDiscontinuedOperations_219886a0-b701-4ca6-b0ac-2971c7eaa9e7" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsIncludingDisposalGroupAndDiscontinuedOperations_e27e1802-ce07-4442-a434-8522020e7dd7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsIncludingDisposalGroupAndDiscontinuedOperations"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SupplementalCashFlowInformationAbstract_36ca3a55-8b4e-4687-9522-4a891a55edd3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SupplementalCashFlowInformationAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_cb1cfce9-fe5b-46c4-8af3-08d437dfded3" xlink:to="loc_us-gaap_SupplementalCashFlowInformationAbstract_36ca3a55-8b4e-4687-9522-4a891a55edd3" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestPaidNet_454f3fb7-69ca-4eea-abf7-3d5b4a625d30" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_InterestPaidNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SupplementalCashFlowInformationAbstract_36ca3a55-8b4e-4687-9522-4a891a55edd3" xlink:to="loc_us-gaap_InterestPaidNet_454f3fb7-69ca-4eea-abf7-3d5b4a625d30" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxesPaidNet_bc1bd2e4-802e-47c5-8447-7440e06cccb5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeTaxesPaidNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SupplementalCashFlowInformationAbstract_36ca3a55-8b4e-4687-9522-4a891a55edd3" xlink:to="loc_us-gaap_IncomeTaxesPaidNet_bc1bd2e4-802e-47c5-8447-7440e06cccb5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashFlowNoncashInvestingAndFinancingActivitiesDisclosureAbstract_681b015a-596c-423d-b5c7-e1f7ad89b64c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CashFlowNoncashInvestingAndFinancingActivitiesDisclosureAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SupplementalCashFlowInformationAbstract_36ca3a55-8b4e-4687-9522-4a891a55edd3" xlink:to="loc_us-gaap_CashFlowNoncashInvestingAndFinancingActivitiesDisclosureAbstract_681b015a-596c-423d-b5c7-e1f7ad89b64c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CapitalExpendituresIncurredButNotYetPaid_979c358f-aabb-44e2-85d7-d36e8dea2e9b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CapitalExpendituresIncurredButNotYetPaid"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashFlowNoncashInvestingAndFinancingActivitiesDisclosureAbstract_681b015a-596c-423d-b5c7-e1f7ad89b64c" xlink:to="loc_us-gaap_CapitalExpendituresIncurredButNotYetPaid_979c358f-aabb-44e2-85d7-d36e8dea2e9b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConsiderationReceivedForBeneficialInterestObtainedForTransferringFinancialAsset_dc4d5c4a-f8b1-4d76-808a-8f50ddb95904" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConsiderationReceivedForBeneficialInterestObtainedForTransferringFinancialAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashFlowNoncashInvestingAndFinancingActivitiesDisclosureAbstract_681b015a-596c-423d-b5c7-e1f7ad89b64c" xlink:to="loc_us-gaap_ConsiderationReceivedForBeneficialInterestObtainedForTransferringFinancialAsset_dc4d5c4a-f8b1-4d76-808a-8f50ddb95904" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/SummaryOfSignificantAccountingPolicies" xlink:type="simple" xlink:href="lnt-20240331.xsd#SummaryOfSignificantAccountingPolicies"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/SummaryOfSignificantAccountingPolicies" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_923aaa14-c34a-4929-854d-6301bd67a4ff" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_dd87124c-a9fb-4fb7-8b0e-abb17c6dcc90" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_923aaa14-c34a-4929-854d-6301bd67a4ff" xlink:to="loc_us-gaap_StatementTable_dd87124c-a9fb-4fb7-8b0e-abb17c6dcc90" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_50ec53a5-3cf5-402d-91e4-e0fd224a568b" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_dd87124c-a9fb-4fb7-8b0e-abb17c6dcc90" xlink:to="loc_dei_LegalEntityAxis_50ec53a5-3cf5-402d-91e4-e0fd224a568b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_50ec53a5-3cf5-402d-91e4-e0fd224a568b_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_50ec53a5-3cf5-402d-91e4-e0fd224a568b" xlink:to="loc_dei_EntityDomain_50ec53a5-3cf5-402d-91e4-e0fd224a568b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_62cac73e-447c-43c2-b5ae-961627e45039" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_50ec53a5-3cf5-402d-91e4-e0fd224a568b" xlink:to="loc_dei_EntityDomain_62cac73e-447c-43c2-b5ae-961627e45039" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_8ef0c20e-1d22-4d31-9ac3-db4d47e6a805" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_62cac73e-447c-43c2-b5ae-961627e45039" xlink:to="loc_lnt_IplMember_8ef0c20e-1d22-4d31-9ac3-db4d47e6a805" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_7643cba9-6fa2-4a34-a592-e4fc16030f72" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_62cac73e-447c-43c2-b5ae-961627e45039" xlink:to="loc_lnt_WplMember_7643cba9-6fa2-4a34-a592-e4fc16030f72" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SignificantAccountingPoliciesTextBlock_db224dfa-1a5f-434b-abe6-d6bd073f2196" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SignificantAccountingPoliciesTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_923aaa14-c34a-4929-854d-6301bd67a4ff" xlink:to="loc_us-gaap_SignificantAccountingPoliciesTextBlock_db224dfa-1a5f-434b-abe6-d6bd073f2196" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/RegulatoryMatters" xlink:type="simple" xlink:href="lnt-20240331.xsd#RegulatoryMatters"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/RegulatoryMatters" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PublicUtilitiesGeneralDisclosuresLineItems_4974684b-d22e-448b-a62f-b49fff05a786" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PublicUtilitiesGeneralDisclosuresLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PublicUtilitiesGeneralDisclosuresTable_ad6b5be0-0b38-4fbc-b011-506e083c45bd" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PublicUtilitiesGeneralDisclosuresTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_PublicUtilitiesGeneralDisclosuresLineItems_4974684b-d22e-448b-a62f-b49fff05a786" xlink:to="loc_us-gaap_PublicUtilitiesGeneralDisclosuresTable_ad6b5be0-0b38-4fbc-b011-506e083c45bd" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_1b35d0e9-96a1-4dbc-86cc-3c71ce895087" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_PublicUtilitiesGeneralDisclosuresTable_ad6b5be0-0b38-4fbc-b011-506e083c45bd" xlink:to="loc_dei_LegalEntityAxis_1b35d0e9-96a1-4dbc-86cc-3c71ce895087" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_1b35d0e9-96a1-4dbc-86cc-3c71ce895087_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_1b35d0e9-96a1-4dbc-86cc-3c71ce895087" xlink:to="loc_dei_EntityDomain_1b35d0e9-96a1-4dbc-86cc-3c71ce895087_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_3a4f3b28-f3b1-48a0-abf0-acf81c0ffeaa" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_1b35d0e9-96a1-4dbc-86cc-3c71ce895087" xlink:to="loc_dei_EntityDomain_3a4f3b28-f3b1-48a0-abf0-acf81c0ffeaa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_c560dd80-ba03-40a2-9719-c42fb8c67470" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_3a4f3b28-f3b1-48a0-abf0-acf81c0ffeaa" xlink:to="loc_lnt_IplMember_c560dd80-ba03-40a2-9719-c42fb8c67470" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_0cf91f72-fc9a-4601-97d8-e209ab434c27" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_3a4f3b28-f3b1-48a0-abf0-acf81c0ffeaa" xlink:to="loc_lnt_WplMember_0cf91f72-fc9a-4601-97d8-e209ab434c27" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PublicUtilitiesDisclosureTextBlock_27a306e1-edb1-4c08-8e63-4cedbab9fe4c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PublicUtilitiesDisclosureTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PublicUtilitiesGeneralDisclosuresLineItems_4974684b-d22e-448b-a62f-b49fff05a786" xlink:to="loc_us-gaap_PublicUtilitiesDisclosureTextBlock_27a306e1-edb1-4c08-8e63-4cedbab9fe4c" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/PropertyPlantandEquipment" xlink:type="simple" xlink:href="lnt-20240331.xsd#PropertyPlantandEquipment"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/PropertyPlantandEquipment" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentLineItems_d3df5cf5-1222-4649-982d-dd32201b90dc" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_abe101b7-a732-413a-8dfd-c25d8e000055" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_d3df5cf5-1222-4649-982d-dd32201b90dc" xlink:to="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_abe101b7-a732-413a-8dfd-c25d8e000055" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_f73f005c-5563-41b6-8a36-e8912e4950d1" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_abe101b7-a732-413a-8dfd-c25d8e000055" xlink:to="loc_dei_LegalEntityAxis_f73f005c-5563-41b6-8a36-e8912e4950d1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_f73f005c-5563-41b6-8a36-e8912e4950d1_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_f73f005c-5563-41b6-8a36-e8912e4950d1" xlink:to="loc_dei_EntityDomain_f73f005c-5563-41b6-8a36-e8912e4950d1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_577d663b-8097-44f2-987e-018f1c4526bc" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_f73f005c-5563-41b6-8a36-e8912e4950d1" xlink:to="loc_dei_EntityDomain_577d663b-8097-44f2-987e-018f1c4526bc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_7b44a0c3-3710-46cc-9132-0e7db9070aa0" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_577d663b-8097-44f2-987e-018f1c4526bc" xlink:to="loc_lnt_IplMember_7b44a0c3-3710-46cc-9132-0e7db9070aa0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_dc648953-f6b5-4ae3-9b2a-4b0ad1a25113" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_577d663b-8097-44f2-987e-018f1c4526bc" xlink:to="loc_lnt_WplMember_dc648953-f6b5-4ae3-9b2a-4b0ad1a25113" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentDisclosureTextBlock_7cd8b2d1-4cc6-4373-a9e9-c15aa5ecd0d5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentDisclosureTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_d3df5cf5-1222-4649-982d-dd32201b90dc" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentDisclosureTextBlock_7cd8b2d1-4cc6-4373-a9e9-c15aa5ecd0d5" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/Receivables" xlink:type="simple" xlink:href="lnt-20240331.xsd#Receivables"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/Receivables" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_e99548c2-bd80-4daf-8087-66cd474333cf" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccountsNotesAndLoansReceivableLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_59ed694f-4e32-4237-a71b-5c3cab2a4a77" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_e99548c2-bd80-4daf-8087-66cd474333cf" xlink:to="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_59ed694f-4e32-4237-a71b-5c3cab2a4a77" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_ae60acaf-50b3-48b9-9a72-a07e9fefadd0" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_59ed694f-4e32-4237-a71b-5c3cab2a4a77" xlink:to="loc_dei_LegalEntityAxis_ae60acaf-50b3-48b9-9a72-a07e9fefadd0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_ae60acaf-50b3-48b9-9a72-a07e9fefadd0_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_ae60acaf-50b3-48b9-9a72-a07e9fefadd0" xlink:to="loc_dei_EntityDomain_ae60acaf-50b3-48b9-9a72-a07e9fefadd0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_d30b152f-e940-4e5e-88e8-ca0d1b3e16ae" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_ae60acaf-50b3-48b9-9a72-a07e9fefadd0" xlink:to="loc_dei_EntityDomain_d30b152f-e940-4e5e-88e8-ca0d1b3e16ae" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_8d271ca3-7dc5-4763-bfdd-87bf09826c8e" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_d30b152f-e940-4e5e-88e8-ca0d1b3e16ae" xlink:to="loc_lnt_IplMember_8d271ca3-7dc5-4763-bfdd-87bf09826c8e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_3afd540d-1b02-417e-8f6d-8731e07afe73" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_d30b152f-e940-4e5e-88e8-ca0d1b3e16ae" xlink:to="loc_lnt_WplMember_3afd540d-1b02-417e-8f6d-8731e07afe73" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LoansNotesTradeAndOtherReceivablesDisclosureTextBlock_e162a031-c2ee-4c85-bde4-23cb0aff1cf5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LoansNotesTradeAndOtherReceivablesDisclosureTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_e99548c2-bd80-4daf-8087-66cd474333cf" xlink:to="loc_us-gaap_LoansNotesTradeAndOtherReceivablesDisclosureTextBlock_e162a031-c2ee-4c85-bde4-23cb0aff1cf5" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/Investments" xlink:type="simple" xlink:href="lnt-20240331.xsd#Investments"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/Investments" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_2cbb24d5-af94-4967-bb50-d42b94b815bc" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfEquityMethodInvestmentsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfEquityMethodInvestmentsTable_37829374-5853-4908-a2e0-786651c2d21a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfEquityMethodInvestmentsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_2cbb24d5-af94-4967-bb50-d42b94b815bc" xlink:to="loc_us-gaap_ScheduleOfEquityMethodInvestmentsTable_37829374-5853-4908-a2e0-786651c2d21a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_06f66461-4937-49ec-ba18-dbc3eebd9440" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfEquityMethodInvestmentsTable_37829374-5853-4908-a2e0-786651c2d21a" xlink:to="loc_dei_LegalEntityAxis_06f66461-4937-49ec-ba18-dbc3eebd9440" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_06f66461-4937-49ec-ba18-dbc3eebd9440_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_06f66461-4937-49ec-ba18-dbc3eebd9440" xlink:to="loc_dei_EntityDomain_06f66461-4937-49ec-ba18-dbc3eebd9440_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_d6a45a31-bb7c-499b-85fd-c9930cbd535a" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_06f66461-4937-49ec-ba18-dbc3eebd9440" xlink:to="loc_dei_EntityDomain_d6a45a31-bb7c-499b-85fd-c9930cbd535a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentHoldingsTextBlock_b1662f49-733e-4a11-adaf-995307fead66" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_InvestmentHoldingsTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_2cbb24d5-af94-4967-bb50-d42b94b815bc" xlink:to="loc_us-gaap_InvestmentHoldingsTextBlock_b1662f49-733e-4a11-adaf-995307fead66" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/CommonEquity" xlink:type="simple" xlink:href="lnt-20240331.xsd#CommonEquity"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/CommonEquity" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_lnt_CommonEquityLineItems_13e9c618-752d-45a5-b673-31caefbe591d" xlink:href="lnt-20240331.xsd#lnt_CommonEquityLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_CommonEquityTable_d5fe32e6-6e3b-4707-8b2e-f6f553693f7c" xlink:href="lnt-20240331.xsd#lnt_CommonEquityTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_lnt_CommonEquityLineItems_13e9c618-752d-45a5-b673-31caefbe591d" xlink:to="loc_lnt_CommonEquityTable_d5fe32e6-6e3b-4707-8b2e-f6f553693f7c" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_5256b7a4-7d14-4d41-b52a-97180a2531ac" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lnt_CommonEquityTable_d5fe32e6-6e3b-4707-8b2e-f6f553693f7c" xlink:to="loc_dei_LegalEntityAxis_5256b7a4-7d14-4d41-b52a-97180a2531ac" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_5256b7a4-7d14-4d41-b52a-97180a2531ac_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_5256b7a4-7d14-4d41-b52a-97180a2531ac" xlink:to="loc_dei_EntityDomain_5256b7a4-7d14-4d41-b52a-97180a2531ac_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_11d34e03-96c5-433c-94c0-374378dba74e" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_5256b7a4-7d14-4d41-b52a-97180a2531ac" xlink:to="loc_dei_EntityDomain_11d34e03-96c5-433c-94c0-374378dba74e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_73b6a340-65d1-44d3-a7af-23d5c431b59a" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_11d34e03-96c5-433c-94c0-374378dba74e" xlink:to="loc_lnt_IplMember_73b6a340-65d1-44d3-a7af-23d5c431b59a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_7b307866-f637-49b4-822d-aa8b7699a72a" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_11d34e03-96c5-433c-94c0-374378dba74e" xlink:to="loc_lnt_WplMember_7b307866-f637-49b4-822d-aa8b7699a72a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityNoteDisclosureTextBlock_2986fc8d-a633-494b-bcde-1ef7fc43e20c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockholdersEquityNoteDisclosureTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lnt_CommonEquityLineItems_13e9c618-752d-45a5-b673-31caefbe591d" xlink:to="loc_us-gaap_StockholdersEquityNoteDisclosureTextBlock_2986fc8d-a633-494b-bcde-1ef7fc43e20c" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/Debt" xlink:type="simple" xlink:href="lnt-20240331.xsd#Debt"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/Debt" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_810b2fc9-478c-4bb7-8d02-739f798dcdb0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_84bbde45-58f3-4c8a-926d-76efc6881e47" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_810b2fc9-478c-4bb7-8d02-739f798dcdb0" xlink:to="loc_us-gaap_DebtInstrumentTable_84bbde45-58f3-4c8a-926d-76efc6881e47" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_26aaa9cf-12cf-4050-ab13-29e387669266" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_84bbde45-58f3-4c8a-926d-76efc6881e47" xlink:to="loc_dei_LegalEntityAxis_26aaa9cf-12cf-4050-ab13-29e387669266" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_26aaa9cf-12cf-4050-ab13-29e387669266_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_26aaa9cf-12cf-4050-ab13-29e387669266" xlink:to="loc_dei_EntityDomain_26aaa9cf-12cf-4050-ab13-29e387669266_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_8ffe4f33-d920-42eb-8949-5e997ace34e1" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_26aaa9cf-12cf-4050-ab13-29e387669266" xlink:to="loc_dei_EntityDomain_8ffe4f33-d920-42eb-8949-5e997ace34e1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_64bc5d81-8da5-45e4-beda-217010300190" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_8ffe4f33-d920-42eb-8949-5e997ace34e1" xlink:to="loc_lnt_IplMember_64bc5d81-8da5-45e4-beda-217010300190" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_40f90099-bdca-4aab-86cc-ae85c411a120" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_8ffe4f33-d920-42eb-8949-5e997ace34e1" xlink:to="loc_lnt_WplMember_40f90099-bdca-4aab-86cc-ae85c411a120" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtDisclosureTextBlock_e7f74567-1392-4bb7-90a9-4aca5f768788" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtDisclosureTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_810b2fc9-478c-4bb7-8d02-739f798dcdb0" xlink:to="loc_us-gaap_DebtDisclosureTextBlock_e7f74567-1392-4bb7-90a9-4aca5f768788" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/Revenues" xlink:type="simple" xlink:href="lnt-20240331.xsd#Revenues"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/Revenues" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueLineItems_1f78ad3a-cf46-4409-9896-68911c84892d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DisaggregationOfRevenueLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueTable_011da009-34c0-4b95-a849-7b1a22cd21a0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DisaggregationOfRevenueTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_1f78ad3a-cf46-4409-9896-68911c84892d" xlink:to="loc_us-gaap_DisaggregationOfRevenueTable_011da009-34c0-4b95-a849-7b1a22cd21a0" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_c5b8afac-06db-452d-808d-a10f760109b3" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_011da009-34c0-4b95-a849-7b1a22cd21a0" xlink:to="loc_dei_LegalEntityAxis_c5b8afac-06db-452d-808d-a10f760109b3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_c5b8afac-06db-452d-808d-a10f760109b3_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_c5b8afac-06db-452d-808d-a10f760109b3" xlink:to="loc_dei_EntityDomain_c5b8afac-06db-452d-808d-a10f760109b3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_ec1fe055-ada5-47b8-b794-da34dfcf0492" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_c5b8afac-06db-452d-808d-a10f760109b3" xlink:to="loc_dei_EntityDomain_ec1fe055-ada5-47b8-b794-da34dfcf0492" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_e4d74418-e6ed-4f5f-9819-5e6bd6a12678" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_ec1fe055-ada5-47b8-b794-da34dfcf0492" xlink:to="loc_lnt_IplMember_e4d74418-e6ed-4f5f-9819-5e6bd6a12678" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_41015096-0de8-4036-8c91-85b7cd1a3c46" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_ec1fe055-ada5-47b8-b794-da34dfcf0492" xlink:to="loc_lnt_WplMember_41015096-0de8-4036-8c91-85b7cd1a3c46" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerTextBlock_b8e76719-5123-4cdd-9ead-c48c4dd77c8b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RevenueFromContractWithCustomerTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_1f78ad3a-cf46-4409-9896-68911c84892d" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerTextBlock_b8e76719-5123-4cdd-9ead-c48c4dd77c8b" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/IncomeTaxes" xlink:type="simple" xlink:href="lnt-20240331.xsd#IncomeTaxes"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/IncomeTaxes" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IncomeTaxLineItems_42b0e4d5-095d-4d88-9635-3dc058ad66e4" xlink:href="lnt-20240331.xsd#lnt_IncomeTaxLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IncomeTaxTable_b1fef69c-9565-45a8-a54d-9e31ac45b4ad" xlink:href="lnt-20240331.xsd#lnt_IncomeTaxTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_lnt_IncomeTaxLineItems_42b0e4d5-095d-4d88-9635-3dc058ad66e4" xlink:to="loc_lnt_IncomeTaxTable_b1fef69c-9565-45a8-a54d-9e31ac45b4ad" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_31af32f2-1e75-4f35-92b5-269ee4ebe79f" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lnt_IncomeTaxTable_b1fef69c-9565-45a8-a54d-9e31ac45b4ad" xlink:to="loc_dei_LegalEntityAxis_31af32f2-1e75-4f35-92b5-269ee4ebe79f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_31af32f2-1e75-4f35-92b5-269ee4ebe79f_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_31af32f2-1e75-4f35-92b5-269ee4ebe79f" xlink:to="loc_dei_EntityDomain_31af32f2-1e75-4f35-92b5-269ee4ebe79f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_f658f951-8202-46b8-8a55-0377833a0d47" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_31af32f2-1e75-4f35-92b5-269ee4ebe79f" xlink:to="loc_dei_EntityDomain_f658f951-8202-46b8-8a55-0377833a0d47" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_d9ddd20c-0a81-4224-904e-2707679d313b" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_f658f951-8202-46b8-8a55-0377833a0d47" xlink:to="loc_lnt_IplMember_d9ddd20c-0a81-4224-904e-2707679d313b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_60198158-85e6-43c0-89f0-bb78f04442d5" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_f658f951-8202-46b8-8a55-0377833a0d47" xlink:to="loc_lnt_WplMember_60198158-85e6-43c0-89f0-bb78f04442d5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxDisclosureTextBlock_55f23e32-0447-4112-8099-7235dd65aa38" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeTaxDisclosureTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lnt_IncomeTaxLineItems_42b0e4d5-095d-4d88-9635-3dc058ad66e4" xlink:to="loc_us-gaap_IncomeTaxDisclosureTextBlock_55f23e32-0447-4112-8099-7235dd65aa38" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/BenefitPlans" xlink:type="simple" xlink:href="lnt-20240331.xsd#BenefitPlans"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/BenefitPlans" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_653cf2d0-1484-48bd-893a-aeeb697bde85" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_0bb50b06-1725-46d6-8799-4c1953c1e8dd" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_653cf2d0-1484-48bd-893a-aeeb697bde85" xlink:to="loc_us-gaap_StatementTable_0bb50b06-1725-46d6-8799-4c1953c1e8dd" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_003fad8a-1f54-4541-b089-f65d2fd72b44" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_0bb50b06-1725-46d6-8799-4c1953c1e8dd" xlink:to="loc_dei_LegalEntityAxis_003fad8a-1f54-4541-b089-f65d2fd72b44" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_003fad8a-1f54-4541-b089-f65d2fd72b44_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_003fad8a-1f54-4541-b089-f65d2fd72b44" xlink:to="loc_dei_EntityDomain_003fad8a-1f54-4541-b089-f65d2fd72b44_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_490e3dc6-0f8d-4202-ba45-8ea58a1697e3" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_003fad8a-1f54-4541-b089-f65d2fd72b44" xlink:to="loc_dei_EntityDomain_490e3dc6-0f8d-4202-ba45-8ea58a1697e3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_e0c327fc-fa4e-4697-8c03-7bc7b94bfacf" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_490e3dc6-0f8d-4202-ba45-8ea58a1697e3" xlink:to="loc_lnt_IplMember_e0c327fc-fa4e-4697-8c03-7bc7b94bfacf" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_6cf88f41-7dad-4344-aadf-ad212f22acad" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_490e3dc6-0f8d-4202-ba45-8ea58a1697e3" xlink:to="loc_lnt_WplMember_6cf88f41-7dad-4344-aadf-ad212f22acad" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CompensationAndEmployeeBenefitPlansTextBlock_c00dda6e-9730-4a51-8ca9-f1a0cd7c090a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CompensationAndEmployeeBenefitPlansTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_653cf2d0-1484-48bd-893a-aeeb697bde85" xlink:to="loc_us-gaap_CompensationAndEmployeeBenefitPlansTextBlock_c00dda6e-9730-4a51-8ca9-f1a0cd7c090a" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/DerivativeInstruments" xlink:type="simple" xlink:href="lnt-20240331.xsd#DerivativeInstruments"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/DerivativeInstruments" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeLineItems_ba140791-c693-42ff-98d0-27a2b7efd998" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeTable_297cf3a4-dbdb-4f3f-b87e-fb75ddfb570b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DerivativeLineItems_ba140791-c693-42ff-98d0-27a2b7efd998" xlink:to="loc_us-gaap_DerivativeTable_297cf3a4-dbdb-4f3f-b87e-fb75ddfb570b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_147c080c-ea26-4ba5-9944-3ba88d9860a0" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeTable_297cf3a4-dbdb-4f3f-b87e-fb75ddfb570b" xlink:to="loc_dei_LegalEntityAxis_147c080c-ea26-4ba5-9944-3ba88d9860a0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_147c080c-ea26-4ba5-9944-3ba88d9860a0_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_147c080c-ea26-4ba5-9944-3ba88d9860a0" xlink:to="loc_dei_EntityDomain_147c080c-ea26-4ba5-9944-3ba88d9860a0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_e4fb0000-ed83-4a32-a74c-f186f342d753" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_147c080c-ea26-4ba5-9944-3ba88d9860a0" xlink:to="loc_dei_EntityDomain_e4fb0000-ed83-4a32-a74c-f186f342d753" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_d29b6580-43ef-484c-8d51-cd2cbdd95bef" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_e4fb0000-ed83-4a32-a74c-f186f342d753" xlink:to="loc_lnt_IplMember_d29b6580-43ef-484c-8d51-cd2cbdd95bef" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_ba996f75-6578-4e9c-8a3d-98955c33e12e" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_e4fb0000-ed83-4a32-a74c-f186f342d753" xlink:to="loc_lnt_WplMember_ba996f75-6578-4e9c-8a3d-98955c33e12e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosureTextBlock_c851a336-8581-4bc8-983b-844bb2b3f13a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosureTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_ba140791-c693-42ff-98d0-27a2b7efd998" xlink:to="loc_us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosureTextBlock_c851a336-8581-4bc8-983b-844bb2b3f13a" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/FairValueMeasurements" xlink:type="simple" xlink:href="lnt-20240331.xsd#FairValueMeasurements"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/FairValueMeasurements" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_86a01a43-872a-4162-abed-d3201468c962" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_e2bce41d-497e-4c42-9f17-320f32651f5b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_86a01a43-872a-4162-abed-d3201468c962" xlink:to="loc_us-gaap_StatementTable_e2bce41d-497e-4c42-9f17-320f32651f5b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_38cfbc68-ad04-43e1-b56d-1f5cd6511775" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_e2bce41d-497e-4c42-9f17-320f32651f5b" xlink:to="loc_dei_LegalEntityAxis_38cfbc68-ad04-43e1-b56d-1f5cd6511775" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_38cfbc68-ad04-43e1-b56d-1f5cd6511775_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_38cfbc68-ad04-43e1-b56d-1f5cd6511775" xlink:to="loc_dei_EntityDomain_38cfbc68-ad04-43e1-b56d-1f5cd6511775_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_d27e73e8-5934-4d87-8449-7d840a45d834" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_38cfbc68-ad04-43e1-b56d-1f5cd6511775" xlink:to="loc_dei_EntityDomain_d27e73e8-5934-4d87-8449-7d840a45d834" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_4a0028b2-5639-4fbc-97f0-40ef2c4d8783" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_d27e73e8-5934-4d87-8449-7d840a45d834" xlink:to="loc_lnt_IplMember_4a0028b2-5639-4fbc-97f0-40ef2c4d8783" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_5e7cff32-d46e-43d2-b4d0-ad0eb3d1999b" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_d27e73e8-5934-4d87-8449-7d840a45d834" xlink:to="loc_lnt_WplMember_5e7cff32-d46e-43d2-b4d0-ad0eb3d1999b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueDisclosuresTextBlock_8610dd5d-8a0a-488c-82d2-6bd3012de54c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueDisclosuresTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_86a01a43-872a-4162-abed-d3201468c962" xlink:to="loc_us-gaap_FairValueDisclosuresTextBlock_8610dd5d-8a0a-488c-82d2-6bd3012de54c" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/CommitmentsAndContingencies" xlink:type="simple" xlink:href="lnt-20240331.xsd#CommitmentsAndContingencies"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/CommitmentsAndContingencies" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_621e0014-79a4-473a-80ad-1b0d87969ce6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_9403f732-d588-4d7c-8039-450423d45fd3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_621e0014-79a4-473a-80ad-1b0d87969ce6" xlink:to="loc_us-gaap_StatementTable_9403f732-d588-4d7c-8039-450423d45fd3" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_7a070b0c-c529-435f-a290-6c8ea5dc85f8" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_9403f732-d588-4d7c-8039-450423d45fd3" xlink:to="loc_dei_LegalEntityAxis_7a070b0c-c529-435f-a290-6c8ea5dc85f8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_7a070b0c-c529-435f-a290-6c8ea5dc85f8_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_7a070b0c-c529-435f-a290-6c8ea5dc85f8" xlink:to="loc_dei_EntityDomain_7a070b0c-c529-435f-a290-6c8ea5dc85f8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_8a856d4b-8897-4888-8825-1e783e4aab9e" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_7a070b0c-c529-435f-a290-6c8ea5dc85f8" xlink:to="loc_dei_EntityDomain_8a856d4b-8897-4888-8825-1e783e4aab9e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_11dad462-34e9-4e23-a570-f2692481b58e" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_8a856d4b-8897-4888-8825-1e783e4aab9e" xlink:to="loc_lnt_IplMember_11dad462-34e9-4e23-a570-f2692481b58e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_46b6ee83-e55a-4dd1-9301-66e1ec7afd79" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_8a856d4b-8897-4888-8825-1e783e4aab9e" xlink:to="loc_lnt_WplMember_46b6ee83-e55a-4dd1-9301-66e1ec7afd79" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommitmentsAndContingenciesDisclosureTextBlock_daef40cf-9b16-41f0-b1bc-41d86c4a2af1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommitmentsAndContingenciesDisclosureTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_621e0014-79a4-473a-80ad-1b0d87969ce6" xlink:to="loc_us-gaap_CommitmentsAndContingenciesDisclosureTextBlock_daef40cf-9b16-41f0-b1bc-41d86c4a2af1" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/SegmentsOfBusiness" xlink:type="simple" xlink:href="lnt-20240331.xsd#SegmentsOfBusiness"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/SegmentsOfBusiness" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentReportingInformationLineItems_e5f5b74b-98ec-400f-bb50-fc94dc2a8a40" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentReportingInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_0c659a47-7842-476c-9706-99f9e5985a5d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_e5f5b74b-98ec-400f-bb50-fc94dc2a8a40" xlink:to="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_0c659a47-7842-476c-9706-99f9e5985a5d" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_9ba4eae5-938f-4894-afd4-2d8ea3086832" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_0c659a47-7842-476c-9706-99f9e5985a5d" xlink:to="loc_dei_LegalEntityAxis_9ba4eae5-938f-4894-afd4-2d8ea3086832" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_9ba4eae5-938f-4894-afd4-2d8ea3086832_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_9ba4eae5-938f-4894-afd4-2d8ea3086832" xlink:to="loc_dei_EntityDomain_9ba4eae5-938f-4894-afd4-2d8ea3086832_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_d3ea1290-80ce-48ab-952e-02895334aece" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_9ba4eae5-938f-4894-afd4-2d8ea3086832" xlink:to="loc_dei_EntityDomain_d3ea1290-80ce-48ab-952e-02895334aece" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_ad061200-0783-422e-b538-d7fb7993b2c5" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_d3ea1290-80ce-48ab-952e-02895334aece" xlink:to="loc_lnt_IplMember_ad061200-0783-422e-b538-d7fb7993b2c5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_66238ebd-c3c6-453f-b6f2-f53f8461616c" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_d3ea1290-80ce-48ab-952e-02895334aece" xlink:to="loc_lnt_WplMember_66238ebd-c3c6-453f-b6f2-f53f8461616c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentReportingDisclosureTextBlock_e83d2c9c-d536-4606-8aa4-ef1a8746d0a7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentReportingDisclosureTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_e5f5b74b-98ec-400f-bb50-fc94dc2a8a40" xlink:to="loc_us-gaap_SegmentReportingDisclosureTextBlock_e83d2c9c-d536-4606-8aa4-ef1a8746d0a7" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/RelatedParties" xlink:type="simple" xlink:href="lnt-20240331.xsd#RelatedParties"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/RelatedParties" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionLineItems_7756d5ec-f951-4ec2-86c4-bb7bc0401234" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyTransactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_735ec8a3-8b6a-4890-9dda-0479b9ff82e0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_7756d5ec-f951-4ec2-86c4-bb7bc0401234" xlink:to="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_735ec8a3-8b6a-4890-9dda-0479b9ff82e0" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_b9e6eab0-5346-4f28-8270-9d5001325702" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_735ec8a3-8b6a-4890-9dda-0479b9ff82e0" xlink:to="loc_dei_LegalEntityAxis_b9e6eab0-5346-4f28-8270-9d5001325702" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_b9e6eab0-5346-4f28-8270-9d5001325702_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_b9e6eab0-5346-4f28-8270-9d5001325702" xlink:to="loc_dei_EntityDomain_b9e6eab0-5346-4f28-8270-9d5001325702_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_512c68ca-dd3a-41b9-b63a-9e91bd1286e6" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_b9e6eab0-5346-4f28-8270-9d5001325702" xlink:to="loc_dei_EntityDomain_512c68ca-dd3a-41b9-b63a-9e91bd1286e6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_1a137447-e21d-4762-aa3e-346d0a8edb54" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_512c68ca-dd3a-41b9-b63a-9e91bd1286e6" xlink:to="loc_lnt_IplMember_1a137447-e21d-4762-aa3e-346d0a8edb54" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_b4d3534a-55c1-4f66-8037-2fa01aa2c383" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_512c68ca-dd3a-41b9-b63a-9e91bd1286e6" xlink:to="loc_lnt_WplMember_b4d3534a-55c1-4f66-8037-2fa01aa2c383" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionsDisclosureTextBlock_5cb3ddff-ab14-4e9d-94e2-b63a77375287" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyTransactionsDisclosureTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_7756d5ec-f951-4ec2-86c4-bb7bc0401234" xlink:to="loc_us-gaap_RelatedPartyTransactionsDisclosureTextBlock_5cb3ddff-ab14-4e9d-94e2-b63a77375287" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/SummaryOfSignificantAccountingPoliciesPolicy" xlink:type="simple" xlink:href="lnt-20240331.xsd#SummaryOfSignificantAccountingPoliciesPolicy"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/SummaryOfSignificantAccountingPoliciesPolicy" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_38527e48-541a-417b-b62f-4582b6cbed56" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_c4d6e0a8-dbea-4e1e-bd78-3851abb5c306" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_38527e48-541a-417b-b62f-4582b6cbed56" xlink:to="loc_us-gaap_StatementTable_c4d6e0a8-dbea-4e1e-bd78-3851abb5c306" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_d243c13e-5e15-4c40-8621-cfe3f6a55758" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_c4d6e0a8-dbea-4e1e-bd78-3851abb5c306" xlink:to="loc_dei_LegalEntityAxis_d243c13e-5e15-4c40-8621-cfe3f6a55758" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_d243c13e-5e15-4c40-8621-cfe3f6a55758_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_d243c13e-5e15-4c40-8621-cfe3f6a55758" xlink:to="loc_dei_EntityDomain_d243c13e-5e15-4c40-8621-cfe3f6a55758_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_fd761d37-fb05-4895-a65d-a393419bca7e" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_d243c13e-5e15-4c40-8621-cfe3f6a55758" xlink:to="loc_dei_EntityDomain_fd761d37-fb05-4895-a65d-a393419bca7e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_03e90c3f-b988-484a-a9f1-84999021e663" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_fd761d37-fb05-4895-a65d-a393419bca7e" xlink:to="loc_lnt_IplMember_03e90c3f-b988-484a-a9f1-84999021e663" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_6bd1192d-be2a-4d74-8632-8d5e154c0608" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_fd761d37-fb05-4895-a65d-a393419bca7e" xlink:to="loc_lnt_WplMember_6bd1192d-be2a-4d74-8632-8d5e154c0608" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BasisOfAccountingPolicyPolicyTextBlock_a78b3d83-251b-487d-92c0-305307d08bb4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_BasisOfAccountingPolicyPolicyTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_38527e48-541a-417b-b62f-4582b6cbed56" xlink:to="loc_us-gaap_BasisOfAccountingPolicyPolicyTextBlock_a78b3d83-251b-487d-92c0-305307d08bb4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UseOfEstimates_8bdc2b32-a2a0-47dc-a39c-16dba12f11b8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_UseOfEstimates"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_38527e48-541a-417b-b62f-4582b6cbed56" xlink:to="loc_us-gaap_UseOfEstimates_8bdc2b32-a2a0-47dc-a39c-16dba12f11b8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PriorPeriodReclassificationAdjustmentDescription_e89bca74-b6c4-4434-824b-7a258cd87782" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PriorPeriodReclassificationAdjustmentDescription"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_38527e48-541a-417b-b62f-4582b6cbed56" xlink:to="loc_us-gaap_PriorPeriodReclassificationAdjustmentDescription_e89bca74-b6c4-4434-824b-7a258cd87782" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/SummaryOfSignificantAccountingPoliciesTables" xlink:type="simple" xlink:href="lnt-20240331.xsd#SummaryOfSignificantAccountingPoliciesTables"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/SummaryOfSignificantAccountingPoliciesTables" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_a5c45ac5-0457-43c7-9998-a4e9b2e66ae4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_c38ac834-12e4-4f05-9e5e-95cabdd4a9a3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_a5c45ac5-0457-43c7-9998-a4e9b2e66ae4" xlink:to="loc_us-gaap_StatementTable_c38ac834-12e4-4f05-9e5e-95cabdd4a9a3" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_3f26799a-b66a-45d9-80cc-de8c48fddb84" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_c38ac834-12e4-4f05-9e5e-95cabdd4a9a3" xlink:to="loc_dei_LegalEntityAxis_3f26799a-b66a-45d9-80cc-de8c48fddb84" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_3f26799a-b66a-45d9-80cc-de8c48fddb84_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_3f26799a-b66a-45d9-80cc-de8c48fddb84" xlink:to="loc_dei_EntityDomain_3f26799a-b66a-45d9-80cc-de8c48fddb84_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_d0b80dd0-f9a8-43bd-8163-3fae999db3a6" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_3f26799a-b66a-45d9-80cc-de8c48fddb84" xlink:to="loc_dei_EntityDomain_d0b80dd0-f9a8-43bd-8163-3fae999db3a6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_1c99014c-2229-4c39-a3a4-35c6b6cce7b5" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_d0b80dd0-f9a8-43bd-8163-3fae999db3a6" xlink:to="loc_lnt_IplMember_1c99014c-2229-4c39-a3a4-35c6b6cce7b5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_a436846d-9dac-4406-8db0-7c9f6bf1c5f3" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_d0b80dd0-f9a8-43bd-8163-3fae999db3a6" xlink:to="loc_lnt_WplMember_a436846d-9dac-4406-8db0-7c9f6bf1c5f3" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/RegulatoryMattersTables" xlink:type="simple" xlink:href="lnt-20240331.xsd#RegulatoryMattersTables"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/RegulatoryMattersTables" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryAssetLineItems_a681aed8-d9b1-458c-95e5-d4edceb8d61e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatoryAssetLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRegulatoryAssetsTable_26930dc2-f81e-42ed-aec8-fc50587c4a10" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfRegulatoryAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RegulatoryAssetLineItems_a681aed8-d9b1-458c-95e5-d4edceb8d61e" xlink:to="loc_us-gaap_ScheduleOfRegulatoryAssetsTable_26930dc2-f81e-42ed-aec8-fc50587c4a10" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_83fcdc3f-8d3a-41fd-8b33-87f33a3ca426" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRegulatoryAssetsTable_26930dc2-f81e-42ed-aec8-fc50587c4a10" xlink:to="loc_dei_LegalEntityAxis_83fcdc3f-8d3a-41fd-8b33-87f33a3ca426" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_83fcdc3f-8d3a-41fd-8b33-87f33a3ca426_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_83fcdc3f-8d3a-41fd-8b33-87f33a3ca426" xlink:to="loc_dei_EntityDomain_83fcdc3f-8d3a-41fd-8b33-87f33a3ca426_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_27c613db-25d5-46b5-963f-820da6dee894" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_83fcdc3f-8d3a-41fd-8b33-87f33a3ca426" xlink:to="loc_dei_EntityDomain_27c613db-25d5-46b5-963f-820da6dee894" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_56f56faa-f2d6-4875-8801-55b2c464bc36" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_27c613db-25d5-46b5-963f-820da6dee894" xlink:to="loc_lnt_IplMember_56f56faa-f2d6-4875-8801-55b2c464bc36" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_809b220f-289c-4c1c-b891-70d106c5192f" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_27c613db-25d5-46b5-963f-820da6dee894" xlink:to="loc_lnt_WplMember_809b220f-289c-4c1c-b891-70d106c5192f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRegulatoryAssetsTextBlock_900080b1-7dba-4147-b846-6ec0757a229e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfRegulatoryAssetsTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryAssetLineItems_a681aed8-d9b1-458c-95e5-d4edceb8d61e" xlink:to="loc_us-gaap_ScheduleOfRegulatoryAssetsTextBlock_900080b1-7dba-4147-b846-6ec0757a229e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRegulatoryLiabilitiesTextBlock_6ab8275a-f1fa-440f-90b3-f42cff9c8395" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfRegulatoryLiabilitiesTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryAssetLineItems_a681aed8-d9b1-458c-95e5-d4edceb8d61e" xlink:to="loc_us-gaap_ScheduleOfRegulatoryLiabilitiesTextBlock_6ab8275a-f1fa-440f-90b3-f42cff9c8395" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/PropertyPlantandEquipmentTables" xlink:type="simple" xlink:href="lnt-20240331.xsd#PropertyPlantandEquipmentTables"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/PropertyPlantandEquipmentTables" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentLineItems_2a7ade8e-dc70-4d6f-b28e-8357e16482e8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_ed76fad8-a562-49df-ae05-c429ddf65d78" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_2a7ade8e-dc70-4d6f-b28e-8357e16482e8" xlink:to="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_ed76fad8-a562-49df-ae05-c429ddf65d78" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_ea5343c9-04ad-449c-9f1a-567ae11b237f" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_ed76fad8-a562-49df-ae05-c429ddf65d78" xlink:to="loc_dei_LegalEntityAxis_ea5343c9-04ad-449c-9f1a-567ae11b237f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_ea5343c9-04ad-449c-9f1a-567ae11b237f_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_ea5343c9-04ad-449c-9f1a-567ae11b237f" xlink:to="loc_dei_EntityDomain_ea5343c9-04ad-449c-9f1a-567ae11b237f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_eb5a7ed2-44e6-45c5-9ee1-f37361464dcb" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_ea5343c9-04ad-449c-9f1a-567ae11b237f" xlink:to="loc_dei_EntityDomain_eb5a7ed2-44e6-45c5-9ee1-f37361464dcb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_fc66c598-e771-4cda-b276-b3ddb2bb75c3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_ed76fad8-a562-49df-ae05-c429ddf65d78" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_fc66c598-e771-4cda-b276-b3ddb2bb75c3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_fc66c598-e771-4cda-b276-b3ddb2bb75c3_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_fc66c598-e771-4cda-b276-b3ddb2bb75c3" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_fc66c598-e771-4cda-b276-b3ddb2bb75c3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_d8d2c67d-2cd7-4d6b-8a07-fd28c401b0ce" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_fc66c598-e771-4cda-b276-b3ddb2bb75c3" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_d8d2c67d-2cd7-4d6b-8a07-fd28c401b0ce" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/ReceivablesTables" xlink:type="simple" xlink:href="lnt-20240331.xsd#ReceivablesTables"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/ReceivablesTables" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_8c564fd6-fe93-48fd-8648-47d6e2cd307b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccountsNotesAndLoansReceivableLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_6a0cc73f-144a-40ac-956d-7e351ec025bb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_8c564fd6-fe93-48fd-8648-47d6e2cd307b" xlink:to="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_6a0cc73f-144a-40ac-956d-7e351ec025bb" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_e18d78e2-9b5a-40ac-8eaf-e67c711b18ce" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_6a0cc73f-144a-40ac-956d-7e351ec025bb" xlink:to="loc_dei_LegalEntityAxis_e18d78e2-9b5a-40ac-8eaf-e67c711b18ce" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_e18d78e2-9b5a-40ac-8eaf-e67c711b18ce_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_e18d78e2-9b5a-40ac-8eaf-e67c711b18ce" xlink:to="loc_dei_EntityDomain_e18d78e2-9b5a-40ac-8eaf-e67c711b18ce_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_0bc0966f-5624-49aa-84c8-59dbd0caf8fb" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_e18d78e2-9b5a-40ac-8eaf-e67c711b18ce" xlink:to="loc_dei_EntityDomain_0bc0966f-5624-49aa-84c8-59dbd0caf8fb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_32149d58-55e9-462e-a906-428b2e4707bc" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_0bc0966f-5624-49aa-84c8-59dbd0caf8fb" xlink:to="loc_lnt_IplMember_32149d58-55e9-462e-a906-428b2e4707bc" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_9e8d8af6-034f-48b7-aabe-010dba58a70a" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_0bc0966f-5624-49aa-84c8-59dbd0caf8fb" xlink:to="loc_lnt_WplMember_9e8d8af6-034f-48b7-aabe-010dba58a70a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_ScheduleOfSalesOfAccountsReceivableCashProceedsAndRelatedCostsTableTextBlock_6b9f7e9f-73a9-44d4-a292-74ead2cf1858" xlink:href="lnt-20240331.xsd#lnt_ScheduleOfSalesOfAccountsReceivableCashProceedsAndRelatedCostsTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_8c564fd6-fe93-48fd-8648-47d6e2cd307b" xlink:to="loc_lnt_ScheduleOfSalesOfAccountsReceivableCashProceedsAndRelatedCostsTableTextBlock_6b9f7e9f-73a9-44d4-a292-74ead2cf1858" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllowanceForCreditLossesOnFinancingReceivablesTableTextBlock_7d62eca6-01e0-489c-9bfe-018b5a7be649" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AllowanceForCreditLossesOnFinancingReceivablesTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_8c564fd6-fe93-48fd-8648-47d6e2cd307b" xlink:to="loc_us-gaap_AllowanceForCreditLossesOnFinancingReceivablesTableTextBlock_7d62eca6-01e0-489c-9bfe-018b5a7be649" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_ScheduleOfAdditionalAttributesOfReceivablesSoldUnderAgreementTableTextBlock_6d50724f-58dc-4aa9-a91a-5cdcfb95c253" xlink:href="lnt-20240331.xsd#lnt_ScheduleOfAdditionalAttributesOfReceivablesSoldUnderAgreementTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_8c564fd6-fe93-48fd-8648-47d6e2cd307b" xlink:to="loc_lnt_ScheduleOfAdditionalAttributesOfReceivablesSoldUnderAgreementTableTextBlock_6d50724f-58dc-4aa9-a91a-5cdcfb95c253" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/InvestmentsTables" xlink:type="simple" xlink:href="lnt-20240331.xsd#InvestmentsTables"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/InvestmentsTables" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_8a8a8e8e-ff8c-4799-92ef-914f86bb7aa2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfEquityMethodInvestmentsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfEquityMethodInvestmentsTable_3a470ace-7fb2-4aa4-8602-7110740a6b78" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfEquityMethodInvestmentsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_8a8a8e8e-ff8c-4799-92ef-914f86bb7aa2" xlink:to="loc_us-gaap_ScheduleOfEquityMethodInvestmentsTable_3a470ace-7fb2-4aa4-8602-7110740a6b78" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_92169ca9-d855-4f14-a2d7-78b7d09b25b5" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfEquityMethodInvestmentsTable_3a470ace-7fb2-4aa4-8602-7110740a6b78" xlink:to="loc_dei_LegalEntityAxis_92169ca9-d855-4f14-a2d7-78b7d09b25b5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_92169ca9-d855-4f14-a2d7-78b7d09b25b5_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_92169ca9-d855-4f14-a2d7-78b7d09b25b5" xlink:to="loc_dei_EntityDomain_92169ca9-d855-4f14-a2d7-78b7d09b25b5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_70fb8309-ae7d-43c6-8749-a76b712a1330" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_92169ca9-d855-4f14-a2d7-78b7d09b25b5" xlink:to="loc_dei_EntityDomain_70fb8309-ae7d-43c6-8749-a76b712a1330" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityMethodInvestmentsTextBlock_5f75fec0-cfc7-4532-8749-2ac87239fe8e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquityMethodInvestmentsTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_8a8a8e8e-ff8c-4799-92ef-914f86bb7aa2" xlink:to="loc_us-gaap_EquityMethodInvestmentsTextBlock_5f75fec0-cfc7-4532-8749-2ac87239fe8e" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/CommonEquityTables" xlink:type="simple" xlink:href="lnt-20240331.xsd#CommonEquityTables"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/CommonEquityTables" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfCapitalizationEquityLineItems_1bc16941-f24e-4a82-8420-6bbf954d757a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfCapitalizationEquityLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfCapitalizationEquityTable_0e33ba14-c170-4cce-bf9d-732f7e92d066" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfCapitalizationEquityTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ScheduleOfCapitalizationEquityLineItems_1bc16941-f24e-4a82-8420-6bbf954d757a" xlink:to="loc_us-gaap_ScheduleOfCapitalizationEquityTable_0e33ba14-c170-4cce-bf9d-732f7e92d066" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_21c8846f-7c04-439a-92fa-1e68e0036c35" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfCapitalizationEquityTable_0e33ba14-c170-4cce-bf9d-732f7e92d066" xlink:to="loc_dei_LegalEntityAxis_21c8846f-7c04-439a-92fa-1e68e0036c35" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_21c8846f-7c04-439a-92fa-1e68e0036c35_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_21c8846f-7c04-439a-92fa-1e68e0036c35" xlink:to="loc_dei_EntityDomain_21c8846f-7c04-439a-92fa-1e68e0036c35_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_2b00233b-aad0-4f56-9f6e-4ed17b553afc" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_21c8846f-7c04-439a-92fa-1e68e0036c35" xlink:to="loc_dei_EntityDomain_2b00233b-aad0-4f56-9f6e-4ed17b553afc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_f4d1ab33-0d58-44ea-a31e-41bfb00240fd" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_2b00233b-aad0-4f56-9f6e-4ed17b553afc" xlink:to="loc_lnt_IplMember_f4d1ab33-0d58-44ea-a31e-41bfb00240fd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_22b7163c-da8d-4397-b49c-40c9c362b7a4" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_2b00233b-aad0-4f56-9f6e-4ed17b553afc" xlink:to="loc_lnt_WplMember_22b7163c-da8d-4397-b49c-40c9c362b7a4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfCapitalUnitsTextBlock_76aa3c39-7cb7-4921-9dad-b037b7a1145e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfCapitalUnitsTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfCapitalizationEquityLineItems_1bc16941-f24e-4a82-8420-6bbf954d757a" xlink:to="loc_us-gaap_ScheduleOfCapitalUnitsTextBlock_76aa3c39-7cb7-4921-9dad-b037b7a1145e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfStockholdersEquityTableTextBlock_807545fa-065f-4aff-9390-6241c4a1d699" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfStockholdersEquityTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfCapitalizationEquityLineItems_1bc16941-f24e-4a82-8420-6bbf954d757a" xlink:to="loc_us-gaap_ScheduleOfStockholdersEquityTableTextBlock_807545fa-065f-4aff-9390-6241c4a1d699" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/DebtTables" xlink:type="simple" xlink:href="lnt-20240331.xsd#DebtTables"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/DebtTables" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShortTermDebtLineItems_57fe20cf-3f90-4d98-9ee4-2a6ddfe7cf45" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShortTermDebtLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShortTermDebtTable_72626a9e-78dc-446a-89e9-b98aaff16604" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfShortTermDebtTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShortTermDebtLineItems_57fe20cf-3f90-4d98-9ee4-2a6ddfe7cf45" xlink:to="loc_us-gaap_ScheduleOfShortTermDebtTable_72626a9e-78dc-446a-89e9-b98aaff16604" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_649d96b4-7c2f-4821-a571-712be46ee81a" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShortTermDebtTable_72626a9e-78dc-446a-89e9-b98aaff16604" xlink:to="loc_dei_LegalEntityAxis_649d96b4-7c2f-4821-a571-712be46ee81a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_649d96b4-7c2f-4821-a571-712be46ee81a_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_649d96b4-7c2f-4821-a571-712be46ee81a" xlink:to="loc_dei_EntityDomain_649d96b4-7c2f-4821-a571-712be46ee81a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_aa67f936-b5b6-4059-a425-13e505d67217" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_649d96b4-7c2f-4821-a571-712be46ee81a" xlink:to="loc_dei_EntityDomain_aa67f936-b5b6-4059-a425-13e505d67217" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_753d5589-d16c-4a23-8493-5a6c287d8544" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_aa67f936-b5b6-4059-a425-13e505d67217" xlink:to="loc_lnt_IplMember_753d5589-d16c-4a23-8493-5a6c287d8544" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_454cfb64-209e-479f-9ca5-f207f3e05e59" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_aa67f936-b5b6-4059-a425-13e505d67217" xlink:to="loc_lnt_WplMember_454cfb64-209e-479f-9ca5-f207f3e05e59" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShortTermDebtTextBlock_706171c5-223c-417a-ba0d-a96b2ebabe86" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfShortTermDebtTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShortTermDebtLineItems_57fe20cf-3f90-4d98-9ee4-2a6ddfe7cf45" xlink:to="loc_us-gaap_ScheduleOfShortTermDebtTextBlock_706171c5-223c-417a-ba0d-a96b2ebabe86" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/RevenuesTables" xlink:type="simple" xlink:href="lnt-20240331.xsd#RevenuesTables"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/RevenuesTables" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueLineItems_3fd4fd02-eb57-4599-9b06-42fcb6fa990c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DisaggregationOfRevenueLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueTable_4e126248-c77c-49ba-a8e1-33af6326c9d1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DisaggregationOfRevenueTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_3fd4fd02-eb57-4599-9b06-42fcb6fa990c" xlink:to="loc_us-gaap_DisaggregationOfRevenueTable_4e126248-c77c-49ba-a8e1-33af6326c9d1" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_70afd066-4acb-464a-85bb-ee5f8c6e1f4b" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_4e126248-c77c-49ba-a8e1-33af6326c9d1" xlink:to="loc_dei_LegalEntityAxis_70afd066-4acb-464a-85bb-ee5f8c6e1f4b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_70afd066-4acb-464a-85bb-ee5f8c6e1f4b_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_70afd066-4acb-464a-85bb-ee5f8c6e1f4b" xlink:to="loc_dei_EntityDomain_70afd066-4acb-464a-85bb-ee5f8c6e1f4b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_42a13469-f38a-483a-af34-6a751f1a5093" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_70afd066-4acb-464a-85bb-ee5f8c6e1f4b" xlink:to="loc_dei_EntityDomain_42a13469-f38a-483a-af34-6a751f1a5093" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_e88f5875-6388-4d61-b3bc-761f44dbbe92" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_42a13469-f38a-483a-af34-6a751f1a5093" xlink:to="loc_lnt_IplMember_e88f5875-6388-4d61-b3bc-761f44dbbe92" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_fc48d2cb-896a-45d1-9539-ac964cfc81a9" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_42a13469-f38a-483a-af34-6a751f1a5093" xlink:to="loc_lnt_WplMember_fc48d2cb-896a-45d1-9539-ac964cfc81a9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueTableTextBlock_0c3b9ed3-f97f-4b7b-9b1b-8d9582c53bdb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DisaggregationOfRevenueTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_3fd4fd02-eb57-4599-9b06-42fcb6fa990c" xlink:to="loc_us-gaap_DisaggregationOfRevenueTableTextBlock_0c3b9ed3-f97f-4b7b-9b1b-8d9582c53bdb" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/IncomeTaxesTables" xlink:type="simple" xlink:href="lnt-20240331.xsd#IncomeTaxesTables"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/IncomeTaxesTables" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IncomeTaxLineItems_26a5513a-558a-421d-a34a-ba765663857f" xlink:href="lnt-20240331.xsd#lnt_IncomeTaxLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IncomeTaxTable_657741c9-51ea-4828-9653-d9268048c89e" xlink:href="lnt-20240331.xsd#lnt_IncomeTaxTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_lnt_IncomeTaxLineItems_26a5513a-558a-421d-a34a-ba765663857f" xlink:to="loc_lnt_IncomeTaxTable_657741c9-51ea-4828-9653-d9268048c89e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_3646b92e-b34e-45ea-be23-3c4d7aa93e15" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lnt_IncomeTaxTable_657741c9-51ea-4828-9653-d9268048c89e" xlink:to="loc_dei_LegalEntityAxis_3646b92e-b34e-45ea-be23-3c4d7aa93e15" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_3646b92e-b34e-45ea-be23-3c4d7aa93e15_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_3646b92e-b34e-45ea-be23-3c4d7aa93e15" xlink:to="loc_dei_EntityDomain_3646b92e-b34e-45ea-be23-3c4d7aa93e15_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_8af0f6ad-bfb1-4700-9b20-44bce74bad8b" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_3646b92e-b34e-45ea-be23-3c4d7aa93e15" xlink:to="loc_dei_EntityDomain_8af0f6ad-bfb1-4700-9b20-44bce74bad8b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_b51689e6-0a4c-43fb-aafb-147b918016be" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_8af0f6ad-bfb1-4700-9b20-44bce74bad8b" xlink:to="loc_lnt_IplMember_b51689e6-0a4c-43fb-aafb-147b918016be" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_8d37678b-031b-4747-aeaf-74c77e9c4ebd" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_8af0f6ad-bfb1-4700-9b20-44bce74bad8b" xlink:to="loc_lnt_WplMember_8d37678b-031b-4747-aeaf-74c77e9c4ebd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfEffectiveIncomeTaxRateReconciliationTableTextBlock_39bb3279-06b9-49c7-924d-292d8627f09b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfEffectiveIncomeTaxRateReconciliationTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lnt_IncomeTaxLineItems_26a5513a-558a-421d-a34a-ba765663857f" xlink:to="loc_us-gaap_ScheduleOfEffectiveIncomeTaxRateReconciliationTableTextBlock_39bb3279-06b9-49c7-924d-292d8627f09b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SummaryOfTaxCreditCarryforwardsTextBlock_87b5e124-cffc-4d6e-8b37-e10a5425fd85" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SummaryOfTaxCreditCarryforwardsTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lnt_IncomeTaxLineItems_26a5513a-558a-421d-a34a-ba765663857f" xlink:to="loc_us-gaap_SummaryOfTaxCreditCarryforwardsTextBlock_87b5e124-cffc-4d6e-8b37-e10a5425fd85" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/BenefitPlansTables" xlink:type="simple" xlink:href="lnt-20240331.xsd#BenefitPlansTables"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/BenefitPlansTables" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_5f58905d-1638-4fbd-8a0f-fb6ea4d25bb8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DefinedBenefitPlanDisclosureLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_c7a304b7-77f1-4013-9836-79227265bb54" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_5f58905d-1638-4fbd-8a0f-fb6ea4d25bb8" xlink:to="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_c7a304b7-77f1-4013-9836-79227265bb54" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_7d3e169f-9aec-4770-913a-727d1c2ea941" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_c7a304b7-77f1-4013-9836-79227265bb54" xlink:to="loc_dei_LegalEntityAxis_7d3e169f-9aec-4770-913a-727d1c2ea941" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_7d3e169f-9aec-4770-913a-727d1c2ea941_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_7d3e169f-9aec-4770-913a-727d1c2ea941" xlink:to="loc_dei_EntityDomain_7d3e169f-9aec-4770-913a-727d1c2ea941_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_65c8239e-5a5e-4ffe-b2be-a6bab050e2d3" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_7d3e169f-9aec-4770-913a-727d1c2ea941" xlink:to="loc_dei_EntityDomain_65c8239e-5a5e-4ffe-b2be-a6bab050e2d3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_b898a6a8-3c76-44f1-bcc5-9cdf32e5ae93" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_65c8239e-5a5e-4ffe-b2be-a6bab050e2d3" xlink:to="loc_lnt_IplMember_b898a6a8-3c76-44f1-bcc5-9cdf32e5ae93" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_e6b8ae77-104b-4621-9998-96e70b96b8a6" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_65c8239e-5a5e-4ffe-b2be-a6bab050e2d3" xlink:to="loc_lnt_WplMember_e6b8ae77-104b-4621-9998-96e70b96b8a6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_1fb41db0-bc34-45a6-9dd9-94ebb2111bd0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_c7a304b7-77f1-4013-9836-79227265bb54" xlink:to="loc_us-gaap_AwardTypeAxis_1fb41db0-bc34-45a6-9dd9-94ebb2111bd0" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_1fb41db0-bc34-45a6-9dd9-94ebb2111bd0_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_1fb41db0-bc34-45a6-9dd9-94ebb2111bd0" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_1fb41db0-bc34-45a6-9dd9-94ebb2111bd0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_3e868177-1fc6-45c9-ad3f-6c611077f947" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_1fb41db0-bc34-45a6-9dd9-94ebb2111bd0" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_3e868177-1fc6-45c9-ad3f-6c611077f947" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfNetBenefitCostsTableTextBlock_6b9970bd-c6ee-4d24-a8d7-9f62b93604f7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfNetBenefitCostsTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_5f58905d-1638-4fbd-8a0f-fb6ea4d25bb8" xlink:to="loc_us-gaap_ScheduleOfNetBenefitCostsTableTextBlock_6b9970bd-c6ee-4d24-a8d7-9f62b93604f7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfEmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsTextBlock_1199a0fe-432c-45ac-a4b9-557743656336" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfEmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_5f58905d-1638-4fbd-8a0f-fb6ea4d25bb8" xlink:to="loc_us-gaap_ScheduleOfEmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsTextBlock_1199a0fe-432c-45ac-a4b9-557743656336" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisclosureOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTextBlock_92f6c07c-7383-4930-8d1a-69d91cf4d413" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DisclosureOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_5f58905d-1638-4fbd-8a0f-fb6ea4d25bb8" xlink:to="loc_us-gaap_DisclosureOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTextBlock_92f6c07c-7383-4930-8d1a-69d91cf4d413" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/DerivativeInstrumentsTables" xlink:type="simple" xlink:href="lnt-20240331.xsd#DerivativeInstrumentsTables"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/DerivativeInstrumentsTables" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeLineItems_457ab1dd-0577-4ee3-8e01-7b132726a2a1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeTable_a34d37a9-4e66-484d-944c-186d41355679" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DerivativeLineItems_457ab1dd-0577-4ee3-8e01-7b132726a2a1" xlink:to="loc_us-gaap_DerivativeTable_a34d37a9-4e66-484d-944c-186d41355679" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_86507647-7e8d-4246-b0e3-a12f4e491430" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeTable_a34d37a9-4e66-484d-944c-186d41355679" xlink:to="loc_dei_LegalEntityAxis_86507647-7e8d-4246-b0e3-a12f4e491430" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_86507647-7e8d-4246-b0e3-a12f4e491430_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_86507647-7e8d-4246-b0e3-a12f4e491430" xlink:to="loc_dei_EntityDomain_86507647-7e8d-4246-b0e3-a12f4e491430_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_dcc2f04b-b9f4-4437-b585-06ea5ad7a47b" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_86507647-7e8d-4246-b0e3-a12f4e491430" xlink:to="loc_dei_EntityDomain_dcc2f04b-b9f4-4437-b585-06ea5ad7a47b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_f49b400a-ce3a-416b-9f1f-eae3a93ec787" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_dcc2f04b-b9f4-4437-b585-06ea5ad7a47b" xlink:to="loc_lnt_IplMember_f49b400a-ce3a-416b-9f1f-eae3a93ec787" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_7547dc79-ecf5-48a5-b390-7c5c35cc5d98" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_dcc2f04b-b9f4-4437-b585-06ea5ad7a47b" xlink:to="loc_lnt_WplMember_7547dc79-ecf5-48a5-b390-7c5c35cc5d98" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfNotionalAmountsOfOutstandingDerivativePositionsTableTextBlock_40d52f37-8959-45fb-ad6e-ce888c60c23b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfNotionalAmountsOfOutstandingDerivativePositionsTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_457ab1dd-0577-4ee3-8e01-7b132726a2a1" xlink:to="loc_us-gaap_ScheduleOfNotionalAmountsOfOutstandingDerivativePositionsTableTextBlock_40d52f37-8959-45fb-ad6e-ce888c60c23b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfDerivativeInstrumentsInStatementOfFinancialPositionFairValueTextBlock_18ab3fef-ced2-4ada-bb3d-08e18bd91ff8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfDerivativeInstrumentsInStatementOfFinancialPositionFairValueTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_457ab1dd-0577-4ee3-8e01-7b132726a2a1" xlink:to="loc_us-gaap_ScheduleOfDerivativeInstrumentsInStatementOfFinancialPositionFairValueTextBlock_18ab3fef-ced2-4ada-bb3d-08e18bd91ff8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OffsettingAssetsTableTextBlock_456497b2-57bf-484b-9adf-dce1e66d0fa1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OffsettingAssetsTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_457ab1dd-0577-4ee3-8e01-7b132726a2a1" xlink:to="loc_us-gaap_OffsettingAssetsTableTextBlock_456497b2-57bf-484b-9adf-dce1e66d0fa1" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/FairValueMeasurementsTables" xlink:type="simple" xlink:href="lnt-20240331.xsd#FairValueMeasurementsTables"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/FairValueMeasurementsTables" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems_53c13c82-8b4b-4da4-8f1d-81c162695a9e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByBalanceSheetGroupingTable_0aa0c5d7-b20d-402e-9cba-0508a9cb42da" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueByBalanceSheetGroupingTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems_53c13c82-8b4b-4da4-8f1d-81c162695a9e" xlink:to="loc_us-gaap_FairValueByBalanceSheetGroupingTable_0aa0c5d7-b20d-402e-9cba-0508a9cb42da" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_80a7565a-eca9-42f3-9258-c65814cffdae" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueByBalanceSheetGroupingTable_0aa0c5d7-b20d-402e-9cba-0508a9cb42da" xlink:to="loc_dei_LegalEntityAxis_80a7565a-eca9-42f3-9258-c65814cffdae" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_80a7565a-eca9-42f3-9258-c65814cffdae_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_80a7565a-eca9-42f3-9258-c65814cffdae" xlink:to="loc_dei_EntityDomain_80a7565a-eca9-42f3-9258-c65814cffdae_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_345877f5-c4f6-468d-b0a2-86536de0b6f5" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_80a7565a-eca9-42f3-9258-c65814cffdae" xlink:to="loc_dei_EntityDomain_345877f5-c4f6-468d-b0a2-86536de0b6f5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_6559adb2-f75d-4ca9-818d-8c9e55208fbd" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_345877f5-c4f6-468d-b0a2-86536de0b6f5" xlink:to="loc_lnt_IplMember_6559adb2-f75d-4ca9-818d-8c9e55208fbd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_c28899ac-c0d0-4806-b038-6607d9a27b63" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_345877f5-c4f6-468d-b0a2-86536de0b6f5" xlink:to="loc_lnt_WplMember_c28899ac-c0d0-4806-b038-6607d9a27b63" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFairValueAssetsAndLiabilitiesMeasuredOnRecurringBasisTableTextBlock_a3ecc42d-8d56-450c-94a8-dd54843825d0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfFairValueAssetsAndLiabilitiesMeasuredOnRecurringBasisTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems_53c13c82-8b4b-4da4-8f1d-81c162695a9e" xlink:to="loc_us-gaap_ScheduleOfFairValueAssetsAndLiabilitiesMeasuredOnRecurringBasisTableTextBlock_a3ecc42d-8d56-450c-94a8-dd54843825d0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationTextBlock_293716be-1585-4d9c-afc5-e4f879704717" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems_53c13c82-8b4b-4da4-8f1d-81c162695a9e" xlink:to="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationTextBlock_293716be-1585-4d9c-afc5-e4f879704717" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_NetDerivativeAssetsAndLiabilitiesTableTextBlock_89bf92d4-3cb3-47b2-8b77-80e4c4a4e2e4" xlink:href="lnt-20240331.xsd#lnt_NetDerivativeAssetsAndLiabilitiesTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems_53c13c82-8b4b-4da4-8f1d-81c162695a9e" xlink:to="loc_lnt_NetDerivativeAssetsAndLiabilitiesTableTextBlock_89bf92d4-3cb3-47b2-8b77-80e4c4a4e2e4" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/CommitmentsAndContingenciesTables" xlink:type="simple" xlink:href="lnt-20240331.xsd#CommitmentsAndContingenciesTables"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/CommitmentsAndContingenciesTables" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermPurchaseCommitmentLineItems_6008789b-3b79-439d-ae79-7dcef05af6f2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermPurchaseCommitmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermPurchaseCommitmentTable_3b8fef72-4581-4a27-afd9-151385185c07" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermPurchaseCommitmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentLineItems_6008789b-3b79-439d-ae79-7dcef05af6f2" xlink:to="loc_us-gaap_LongTermPurchaseCommitmentTable_3b8fef72-4581-4a27-afd9-151385185c07" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_9d0e2d66-8762-4973-bdf1-6fbd74c6bae3" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentTable_3b8fef72-4581-4a27-afd9-151385185c07" xlink:to="loc_dei_LegalEntityAxis_9d0e2d66-8762-4973-bdf1-6fbd74c6bae3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_9d0e2d66-8762-4973-bdf1-6fbd74c6bae3_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_9d0e2d66-8762-4973-bdf1-6fbd74c6bae3" xlink:to="loc_dei_EntityDomain_9d0e2d66-8762-4973-bdf1-6fbd74c6bae3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_16fd0bf7-a246-445b-88bd-c2fe1ee81fbf" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_9d0e2d66-8762-4973-bdf1-6fbd74c6bae3" xlink:to="loc_dei_EntityDomain_16fd0bf7-a246-445b-88bd-c2fe1ee81fbf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_48a3bbee-8168-4652-86a0-4411d3ee3123" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_16fd0bf7-a246-445b-88bd-c2fe1ee81fbf" xlink:to="loc_lnt_IplMember_48a3bbee-8168-4652-86a0-4411d3ee3123" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_1757057e-a770-406b-a714-608894909726" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_16fd0bf7-a246-445b-88bd-c2fe1ee81fbf" xlink:to="loc_lnt_WplMember_1757057e-a770-406b-a714-608894909726" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermPurchaseCommitmentTextBlock_ad1834eb-652c-4af1-9e3b-f5174ed26781" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermPurchaseCommitmentTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentLineItems_6008789b-3b79-439d-ae79-7dcef05af6f2" xlink:to="loc_us-gaap_LongTermPurchaseCommitmentTextBlock_ad1834eb-652c-4af1-9e3b-f5174ed26781" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EnvironmentalExitCostsByCostTextBlock_3489390a-8c6f-4f81-8f56-04d4d9a71b5b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EnvironmentalExitCostsByCostTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentLineItems_6008789b-3b79-439d-ae79-7dcef05af6f2" xlink:to="loc_us-gaap_EnvironmentalExitCostsByCostTextBlock_3489390a-8c6f-4f81-8f56-04d4d9a71b5b" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/SegmentsOfBusinessTables" xlink:type="simple" xlink:href="lnt-20240331.xsd#SegmentsOfBusinessTables"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/SegmentsOfBusinessTables" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentReportingInformationLineItems_770fe42c-ae99-4943-9eed-44490f0245aa" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentReportingInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_0ed26af9-6dbe-4cd0-b531-a502f90145b5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_770fe42c-ae99-4943-9eed-44490f0245aa" xlink:to="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_0ed26af9-6dbe-4cd0-b531-a502f90145b5" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_9591da62-939d-4255-96b7-8f1b2cf90ca8" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_0ed26af9-6dbe-4cd0-b531-a502f90145b5" xlink:to="loc_dei_LegalEntityAxis_9591da62-939d-4255-96b7-8f1b2cf90ca8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_9591da62-939d-4255-96b7-8f1b2cf90ca8_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_9591da62-939d-4255-96b7-8f1b2cf90ca8" xlink:to="loc_dei_EntityDomain_9591da62-939d-4255-96b7-8f1b2cf90ca8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_275c00e3-d313-4c3b-a7d8-e1e33ceb585f" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_9591da62-939d-4255-96b7-8f1b2cf90ca8" xlink:to="loc_dei_EntityDomain_275c00e3-d313-4c3b-a7d8-e1e33ceb585f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_b59035b2-6e69-42bd-b93c-473e0a3faf0d" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_275c00e3-d313-4c3b-a7d8-e1e33ceb585f" xlink:to="loc_lnt_IplMember_b59035b2-6e69-42bd-b93c-473e0a3faf0d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_7c26351d-0360-4f72-88f1-4b2fa6425a88" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_275c00e3-d313-4c3b-a7d8-e1e33ceb585f" xlink:to="loc_lnt_WplMember_7c26351d-0360-4f72-88f1-4b2fa6425a88" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTextBlock_7c463523-4b60-4e0b-b33c-e03c8c13c946" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfSegmentReportingInformationBySegmentTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_770fe42c-ae99-4943-9eed-44490f0245aa" xlink:to="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTextBlock_7c463523-4b60-4e0b-b33c-e03c8c13c946" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/RelatedPartiesTables" xlink:type="simple" xlink:href="lnt-20240331.xsd#RelatedPartiesTables"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/RelatedPartiesTables" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionLineItems_09a03d80-7262-4435-9286-ebbd744dd500" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyTransactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_20501357-dad3-406a-975b-7dba5c90f549" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_09a03d80-7262-4435-9286-ebbd744dd500" xlink:to="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_20501357-dad3-406a-975b-7dba5c90f549" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_c03935c7-76a9-4660-a91e-c11db122eeec" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_20501357-dad3-406a-975b-7dba5c90f549" xlink:to="loc_dei_LegalEntityAxis_c03935c7-76a9-4660-a91e-c11db122eeec" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_c03935c7-76a9-4660-a91e-c11db122eeec_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_c03935c7-76a9-4660-a91e-c11db122eeec" xlink:to="loc_dei_EntityDomain_c03935c7-76a9-4660-a91e-c11db122eeec_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_2ac86263-74e7-4ca6-b286-83b3bf0fb345" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_c03935c7-76a9-4660-a91e-c11db122eeec" xlink:to="loc_dei_EntityDomain_2ac86263-74e7-4ca6-b286-83b3bf0fb345" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_cc7b3e58-af20-4d4d-ad00-a6cf3ffc7097" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_2ac86263-74e7-4ca6-b286-83b3bf0fb345" xlink:to="loc_lnt_IplMember_cc7b3e58-af20-4d4d-ad00-a6cf3ffc7097" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_899fee95-2a54-44cd-a39f-4d0effad5a3c" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_2ac86263-74e7-4ca6-b286-83b3bf0fb345" xlink:to="loc_lnt_WplMember_899fee95-2a54-44cd-a39f-4d0effad5a3c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_ScheduleOfScoaSalesCreditedAndPurchasesBilledTableTextBlock_c06927d5-6ad8-439a-870f-7b9990b763e2" xlink:href="lnt-20240331.xsd#lnt_ScheduleOfScoaSalesCreditedAndPurchasesBilledTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_09a03d80-7262-4435-9286-ebbd744dd500" xlink:to="loc_lnt_ScheduleOfScoaSalesCreditedAndPurchasesBilledTableTextBlock_c06927d5-6ad8-439a-870f-7b9990b763e2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_ScheduleOfCorporateServicesIntercompanyPayablesTableTextBlock_bc626080-18ea-466f-81f4-f17da820a95a" xlink:href="lnt-20240331.xsd#lnt_ScheduleOfCorporateServicesIntercompanyPayablesTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_09a03d80-7262-4435-9286-ebbd744dd500" xlink:to="loc_lnt_ScheduleOfCorporateServicesIntercompanyPayablesTableTextBlock_bc626080-18ea-466f-81f4-f17da820a95a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_ScheduleOfAtcBillingsTableTextBlock_97f4618c-e6c8-4a08-9a1d-17e3f53a2b76" xlink:href="lnt-20240331.xsd#lnt_ScheduleOfAtcBillingsTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_09a03d80-7262-4435-9286-ebbd744dd500" xlink:to="loc_lnt_ScheduleOfAtcBillingsTableTextBlock_97f4618c-e6c8-4a08-9a1d-17e3f53a2b76" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/SummaryOfSignificantAccountingPoliciesNarrativeDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#SummaryOfSignificantAccountingPoliciesNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/SummaryOfSignificantAccountingPoliciesNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleLineItems_549776a1-eca4-45d8-8f10-487de4461218" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleTable_b59c317e-23c1-4b6b-9fc0-0d79b753d63f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleLineItems_549776a1-eca4-45d8-8f10-487de4461218" xlink:to="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleTable_b59c317e-23c1-4b6b-9fc0-0d79b753d63f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_1a244e6b-b38f-415e-82af-d5a0291b0ea6" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_NewAccountingPronouncementsOrChangeInAccountingPrincipleTable_b59c317e-23c1-4b6b-9fc0-0d79b753d63f" xlink:to="loc_dei_LegalEntityAxis_1a244e6b-b38f-415e-82af-d5a0291b0ea6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_1a244e6b-b38f-415e-82af-d5a0291b0ea6_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_1a244e6b-b38f-415e-82af-d5a0291b0ea6" xlink:to="loc_dei_EntityDomain_1a244e6b-b38f-415e-82af-d5a0291b0ea6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_2eddca30-3905-4555-ae31-7625d53d8a95" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_1a244e6b-b38f-415e-82af-d5a0291b0ea6" xlink:to="loc_dei_EntityDomain_2eddca30-3905-4555-ae31-7625d53d8a95" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_0d11fbe1-1d70-4c61-8278-a846253937e1" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_2eddca30-3905-4555-ae31-7625d53d8a95" xlink:to="loc_lnt_IplMember_0d11fbe1-1d70-4c61-8278-a846253937e1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_780d5cbc-5d71-4af6-a386-16b85849cdf0" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_2eddca30-3905-4555-ae31-7625d53d8a95" xlink:to="loc_lnt_WplMember_780d5cbc-5d71-4af6-a386-16b85849cdf0" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/RegulatoryMattersNarrativeDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#RegulatoryMattersNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/RegulatoryMattersNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_lnt_RegulatoryMattersLineItems_e6ba505b-d4aa-4c8c-911d-0a12fe25e0d9" xlink:href="lnt-20240331.xsd#lnt_RegulatoryMattersLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_RetailRequestTable_25b90640-2705-4092-b0c0-3a04d7877313" xlink:href="lnt-20240331.xsd#lnt_RetailRequestTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_lnt_RegulatoryMattersLineItems_e6ba505b-d4aa-4c8c-911d-0a12fe25e0d9" xlink:to="loc_lnt_RetailRequestTable_25b90640-2705-4092-b0c0-3a04d7877313" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryAssetAxis_d55c8e2b-6163-4d11-b07d-853b57bb0a12" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatoryAssetAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lnt_RetailRequestTable_25b90640-2705-4092-b0c0-3a04d7877313" xlink:to="loc_us-gaap_RegulatoryAssetAxis_d55c8e2b-6163-4d11-b07d-853b57bb0a12" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryAssetDomain_d55c8e2b-6163-4d11-b07d-853b57bb0a12_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatoryAssetDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RegulatoryAssetAxis_d55c8e2b-6163-4d11-b07d-853b57bb0a12" xlink:to="loc_us-gaap_RegulatoryAssetDomain_d55c8e2b-6163-4d11-b07d-853b57bb0a12_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryAssetDomain_7e550df7-f00c-47d2-89aa-c3b228da7015" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatoryAssetDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RegulatoryAssetAxis_d55c8e2b-6163-4d11-b07d-853b57bb0a12" xlink:to="loc_us-gaap_RegulatoryAssetDomain_7e550df7-f00c-47d2-89aa-c3b228da7015" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityAxis_3ced118d-9158-42e9-b91a-ecce12e7e2d3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeTaxAuthorityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lnt_RetailRequestTable_25b90640-2705-4092-b0c0-3a04d7877313" xlink:to="loc_us-gaap_IncomeTaxAuthorityAxis_3ced118d-9158-42e9-b91a-ecce12e7e2d3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityDomain_3ced118d-9158-42e9-b91a-ecce12e7e2d3_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeTaxAuthorityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeTaxAuthorityAxis_3ced118d-9158-42e9-b91a-ecce12e7e2d3" xlink:to="loc_us-gaap_IncomeTaxAuthorityDomain_3ced118d-9158-42e9-b91a-ecce12e7e2d3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityDomain_20f4d506-cdd8-48c2-acda-63cd3c545d59" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeTaxAuthorityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeTaxAuthorityAxis_3ced118d-9158-42e9-b91a-ecce12e7e2d3" xlink:to="loc_us-gaap_IncomeTaxAuthorityDomain_20f4d506-cdd8-48c2-acda-63cd3c545d59" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryLiabilityAxis_883700fa-8d7f-4f34-912c-44ef0e90a17a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatoryLiabilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lnt_RetailRequestTable_25b90640-2705-4092-b0c0-3a04d7877313" xlink:to="loc_us-gaap_RegulatoryLiabilityAxis_883700fa-8d7f-4f34-912c-44ef0e90a17a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryLiabilityDomain_883700fa-8d7f-4f34-912c-44ef0e90a17a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatoryLiabilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RegulatoryLiabilityAxis_883700fa-8d7f-4f34-912c-44ef0e90a17a" xlink:to="loc_us-gaap_RegulatoryLiabilityDomain_883700fa-8d7f-4f34-912c-44ef0e90a17a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryLiabilityDomain_62a4723d-5fc7-497e-8597-7cd45e02584f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatoryLiabilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RegulatoryLiabilityAxis_883700fa-8d7f-4f34-912c-44ef0e90a17a" xlink:to="loc_us-gaap_RegulatoryLiabilityDomain_62a4723d-5fc7-497e-8597-7cd45e02584f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementScenarioAxis_0e6fc03c-4bde-49b9-9115-3554e3655cd7" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_StatementScenarioAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lnt_RetailRequestTable_25b90640-2705-4092-b0c0-3a04d7877313" xlink:to="loc_srt_StatementScenarioAxis_0e6fc03c-4bde-49b9-9115-3554e3655cd7" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_0e6fc03c-4bde-49b9-9115-3554e3655cd7_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementScenarioAxis_0e6fc03c-4bde-49b9-9115-3554e3655cd7" xlink:to="loc_srt_ScenarioUnspecifiedDomain_0e6fc03c-4bde-49b9-9115-3554e3655cd7_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_a617e967-383d-4ac3-aea0-e430812d861e" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementScenarioAxis_0e6fc03c-4bde-49b9-9115-3554e3655cd7" xlink:to="loc_srt_ScenarioUnspecifiedDomain_a617e967-383d-4ac3-aea0-e430812d861e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeAxis_615c4073-8d59-417b-8df8-bf7cf9125622" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsequentEventTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lnt_RetailRequestTable_25b90640-2705-4092-b0c0-3a04d7877313" xlink:to="loc_us-gaap_SubsequentEventTypeAxis_615c4073-8d59-417b-8df8-bf7cf9125622" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_615c4073-8d59-417b-8df8-bf7cf9125622_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_615c4073-8d59-417b-8df8-bf7cf9125622" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_615c4073-8d59-417b-8df8-bf7cf9125622_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_afae0390-7cb3-4ccd-bbd6-48c50b097cbd" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_615c4073-8d59-417b-8df8-bf7cf9125622" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_afae0390-7cb3-4ccd-bbd6-48c50b097cbd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PublicUtilitiesRegulatoryProceedingAxis_a0db0d59-cbfe-4bfb-8014-91a65e9ff60b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PublicUtilitiesRegulatoryProceedingAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lnt_RetailRequestTable_25b90640-2705-4092-b0c0-3a04d7877313" xlink:to="loc_us-gaap_PublicUtilitiesRegulatoryProceedingAxis_a0db0d59-cbfe-4bfb-8014-91a65e9ff60b" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PublicUtilitiesRegulatoryProceedingDomain_a0db0d59-cbfe-4bfb-8014-91a65e9ff60b_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PublicUtilitiesRegulatoryProceedingDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PublicUtilitiesRegulatoryProceedingAxis_a0db0d59-cbfe-4bfb-8014-91a65e9ff60b" xlink:to="loc_us-gaap_PublicUtilitiesRegulatoryProceedingDomain_a0db0d59-cbfe-4bfb-8014-91a65e9ff60b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PublicUtilitiesRegulatoryProceedingDomain_43a62f86-4023-4f3b-b857-8c0c8e62cbc6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PublicUtilitiesRegulatoryProceedingDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PublicUtilitiesRegulatoryProceedingAxis_a0db0d59-cbfe-4bfb-8014-91a65e9ff60b" xlink:to="loc_us-gaap_PublicUtilitiesRegulatoryProceedingDomain_43a62f86-4023-4f3b-b857-8c0c8e62cbc6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PublicUtilityAxis_0d9c48ec-fb45-40fe-a5ad-a14071eee492" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PublicUtilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lnt_RetailRequestTable_25b90640-2705-4092-b0c0-3a04d7877313" xlink:to="loc_us-gaap_PublicUtilityAxis_0d9c48ec-fb45-40fe-a5ad-a14071eee492" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UtilityPlantDomain_0d9c48ec-fb45-40fe-a5ad-a14071eee492_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_UtilityPlantDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PublicUtilityAxis_0d9c48ec-fb45-40fe-a5ad-a14071eee492" xlink:to="loc_us-gaap_UtilityPlantDomain_0d9c48ec-fb45-40fe-a5ad-a14071eee492_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UtilityPlantDomain_4e6df5f9-085d-46af-b944-93e6c39f3685" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_UtilityPlantDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PublicUtilityAxis_0d9c48ec-fb45-40fe-a5ad-a14071eee492" xlink:to="loc_us-gaap_UtilityPlantDomain_4e6df5f9-085d-46af-b944-93e6c39f3685" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_cfa9ddc9-a94b-475b-9dd5-ce1204fb847b" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lnt_RetailRequestTable_25b90640-2705-4092-b0c0-3a04d7877313" xlink:to="loc_dei_LegalEntityAxis_cfa9ddc9-a94b-475b-9dd5-ce1204fb847b" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_cfa9ddc9-a94b-475b-9dd5-ce1204fb847b_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_cfa9ddc9-a94b-475b-9dd5-ce1204fb847b" xlink:to="loc_dei_EntityDomain_cfa9ddc9-a94b-475b-9dd5-ce1204fb847b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_2d3871d4-f630-4528-8b3a-3cd0c49c4df0" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_cfa9ddc9-a94b-475b-9dd5-ce1204fb847b" xlink:to="loc_dei_EntityDomain_2d3871d4-f630-4528-8b3a-3cd0c49c4df0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_8e003c49-db5a-422e-93df-cdebd60d9495" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_2d3871d4-f630-4528-8b3a-3cd0c49c4df0" xlink:to="loc_lnt_IplMember_8e003c49-db5a-422e-93df-cdebd60d9495" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_9141231b-c61c-489f-ac3d-868ca2fbdbed" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_2d3871d4-f630-4528-8b3a-3cd0c49c4df0" xlink:to="loc_lnt_WplMember_9141231b-c61c-489f-ac3d-868ca2fbdbed" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/RegulatoryMattersRegulatoryAssetsDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#RegulatoryMattersRegulatoryAssetsDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/RegulatoryMattersRegulatoryAssetsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryAssetLineItems_b94e2724-dc73-4b4f-a5ce-7b8b090fceaf" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatoryAssetLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRegulatoryAssetsTable_f9f7e3d0-7211-4b79-ab41-2d532c81c9cd" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfRegulatoryAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RegulatoryAssetLineItems_b94e2724-dc73-4b4f-a5ce-7b8b090fceaf" xlink:to="loc_us-gaap_ScheduleOfRegulatoryAssetsTable_f9f7e3d0-7211-4b79-ab41-2d532c81c9cd" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_96e38560-f741-4b40-95bf-319aeb229a2f" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRegulatoryAssetsTable_f9f7e3d0-7211-4b79-ab41-2d532c81c9cd" xlink:to="loc_dei_LegalEntityAxis_96e38560-f741-4b40-95bf-319aeb229a2f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_96e38560-f741-4b40-95bf-319aeb229a2f_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_96e38560-f741-4b40-95bf-319aeb229a2f" xlink:to="loc_dei_EntityDomain_96e38560-f741-4b40-95bf-319aeb229a2f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_18818ba5-6c16-41aa-b5a0-a274b7b49b19" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_96e38560-f741-4b40-95bf-319aeb229a2f" xlink:to="loc_dei_EntityDomain_18818ba5-6c16-41aa-b5a0-a274b7b49b19" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_9654610e-7068-4a70-8047-0382016368ca" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_18818ba5-6c16-41aa-b5a0-a274b7b49b19" xlink:to="loc_lnt_IplMember_9654610e-7068-4a70-8047-0382016368ca" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_56740102-378e-44ca-b49e-9961e9b6e1b3" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_18818ba5-6c16-41aa-b5a0-a274b7b49b19" xlink:to="loc_lnt_WplMember_56740102-378e-44ca-b49e-9961e9b6e1b3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryAssetAxis_79a5260d-e977-427f-99b0-9458bc7265c5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatoryAssetAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRegulatoryAssetsTable_f9f7e3d0-7211-4b79-ab41-2d532c81c9cd" xlink:to="loc_us-gaap_RegulatoryAssetAxis_79a5260d-e977-427f-99b0-9458bc7265c5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryAssetDomain_79a5260d-e977-427f-99b0-9458bc7265c5_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatoryAssetDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RegulatoryAssetAxis_79a5260d-e977-427f-99b0-9458bc7265c5" xlink:to="loc_us-gaap_RegulatoryAssetDomain_79a5260d-e977-427f-99b0-9458bc7265c5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryAssetDomain_80469d9e-cf3c-439b-9ee4-e6608f5b29fc" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatoryAssetDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RegulatoryAssetAxis_79a5260d-e977-427f-99b0-9458bc7265c5" xlink:to="loc_us-gaap_RegulatoryAssetDomain_80469d9e-cf3c-439b-9ee4-e6608f5b29fc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredIncomeTaxChargesMember_e6da7557-9789-441a-b255-195e68a8e8fb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DeferredIncomeTaxChargesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryAssetDomain_80469d9e-cf3c-439b-9ee4-e6608f5b29fc" xlink:to="loc_us-gaap_DeferredIncomeTaxChargesMember_e6da7557-9789-441a-b255-195e68a8e8fb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PensionAndOtherPostretirementPlansCostsMember_7e7bab53-c592-40c2-8b78-4a20e90cfec9" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PensionAndOtherPostretirementPlansCostsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryAssetDomain_80469d9e-cf3c-439b-9ee4-e6608f5b29fc" xlink:to="loc_us-gaap_PensionAndOtherPostretirementPlansCostsMember_7e7bab53-c592-40c2-8b78-4a20e90cfec9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_AssetsRetiredEarlyMember_8ebd2cf8-8db0-4458-9cd6-c02c78e380c8" xlink:href="lnt-20240331.xsd#lnt_AssetsRetiredEarlyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryAssetDomain_80469d9e-cf3c-439b-9ee4-e6608f5b29fc" xlink:to="loc_lnt_AssetsRetiredEarlyMember_8ebd2cf8-8db0-4458-9cd6-c02c78e380c8" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetRetirementObligationCostsMember_60e89ab4-f082-4bee-9f87-c8f696a83ab1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AssetRetirementObligationCostsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryAssetDomain_80469d9e-cf3c-439b-9ee4-e6608f5b29fc" xlink:to="loc_us-gaap_AssetRetirementObligationCostsMember_60e89ab4-f082-4bee-9f87-c8f696a83ab1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_CommodityCostRecoveryMember_1c4d618a-cba4-4f3a-aa90-4a97af06e5b6" xlink:href="lnt-20240331.xsd#lnt_CommodityCostRecoveryMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryAssetDomain_80469d9e-cf3c-439b-9ee4-e6608f5b29fc" xlink:to="loc_lnt_CommodityCostRecoveryMember_1c4d618a-cba4-4f3a-aa90-4a97af06e5b6" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredDerivativeGainLossMember_2bae69d0-6fef-47e7-9af3-a60528a79315" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DeferredDerivativeGainLossMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryAssetDomain_80469d9e-cf3c-439b-9ee4-e6608f5b29fc" xlink:to="loc_us-gaap_DeferredDerivativeGainLossMember_2bae69d0-6fef-47e7-9af3-a60528a79315" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WesternWisconsinGasDistributionExpansionInvestmentsMember_7007d66c-387a-451f-bf1c-9e4c15a899ad" xlink:href="lnt-20240331.xsd#lnt_WesternWisconsinGasDistributionExpansionInvestmentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryAssetDomain_80469d9e-cf3c-439b-9ee4-e6608f5b29fc" xlink:to="loc_lnt_WesternWisconsinGasDistributionExpansionInvestmentsMember_7007d66c-387a-451f-bf1c-9e4c15a899ad" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_DAECPPAMember_faf58b2c-2e79-4127-8d96-dae7d59ee2fc" xlink:href="lnt-20240331.xsd#lnt_DAECPPAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryAssetDomain_80469d9e-cf3c-439b-9ee4-e6608f5b29fc" xlink:to="loc_lnt_DAECPPAMember_faf58b2c-2e79-4127-8d96-dae7d59ee2fc" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherRegulatoryAssetsLiabilitiesMember_58f7dc8d-0cb3-4ae7-9d2f-18e04ff0551d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherRegulatoryAssetsLiabilitiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryAssetDomain_80469d9e-cf3c-439b-9ee4-e6608f5b29fc" xlink:to="loc_us-gaap_OtherRegulatoryAssetsLiabilitiesMember_58f7dc8d-0cb3-4ae7-9d2f-18e04ff0551d" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryAssets_7d6b6d8e-b065-423f-bbdd-fc4a633963d0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatoryAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryAssetLineItems_b94e2724-dc73-4b4f-a5ce-7b8b090fceaf" xlink:to="loc_us-gaap_RegulatoryAssets_7d6b6d8e-b065-423f-bbdd-fc4a633963d0" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/RegulatoryMattersRegulatoryLiabilitiesDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#RegulatoryMattersRegulatoryLiabilitiesDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/RegulatoryMattersRegulatoryLiabilitiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryLiabilitiesLineItems_7f7d754a-8f46-4a24-8442-a4c93367ab95" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatoryLiabilitiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRegulatoryLiabilitiesTable_76adaa67-044f-49dc-97c2-d5819cd36980" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfRegulatoryLiabilitiesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RegulatoryLiabilitiesLineItems_7f7d754a-8f46-4a24-8442-a4c93367ab95" xlink:to="loc_us-gaap_ScheduleOfRegulatoryLiabilitiesTable_76adaa67-044f-49dc-97c2-d5819cd36980" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_0ed349f9-5f95-4f6f-afcb-6d63fc2f664b" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRegulatoryLiabilitiesTable_76adaa67-044f-49dc-97c2-d5819cd36980" xlink:to="loc_dei_LegalEntityAxis_0ed349f9-5f95-4f6f-afcb-6d63fc2f664b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_0ed349f9-5f95-4f6f-afcb-6d63fc2f664b_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_0ed349f9-5f95-4f6f-afcb-6d63fc2f664b" xlink:to="loc_dei_EntityDomain_0ed349f9-5f95-4f6f-afcb-6d63fc2f664b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_cf5caf4f-3357-43dc-8f47-7e4ebc37a23d" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_0ed349f9-5f95-4f6f-afcb-6d63fc2f664b" xlink:to="loc_dei_EntityDomain_cf5caf4f-3357-43dc-8f47-7e4ebc37a23d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_e1d8c96d-fad5-43e7-857d-c8fea498629c" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_cf5caf4f-3357-43dc-8f47-7e4ebc37a23d" xlink:to="loc_lnt_IplMember_e1d8c96d-fad5-43e7-857d-c8fea498629c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_baa4cd3f-5725-4927-940e-5ff854aa2c02" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_cf5caf4f-3357-43dc-8f47-7e4ebc37a23d" xlink:to="loc_lnt_WplMember_baa4cd3f-5725-4927-940e-5ff854aa2c02" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryLiabilityAxis_9ca85dda-916e-4d77-a70e-bdb10ef85001" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatoryLiabilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRegulatoryLiabilitiesTable_76adaa67-044f-49dc-97c2-d5819cd36980" xlink:to="loc_us-gaap_RegulatoryLiabilityAxis_9ca85dda-916e-4d77-a70e-bdb10ef85001" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryLiabilityDomain_9ca85dda-916e-4d77-a70e-bdb10ef85001_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatoryLiabilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RegulatoryLiabilityAxis_9ca85dda-916e-4d77-a70e-bdb10ef85001" xlink:to="loc_us-gaap_RegulatoryLiabilityDomain_9ca85dda-916e-4d77-a70e-bdb10ef85001_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryLiabilityDomain_85137647-87ff-4abd-964f-9fee10fd459e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatoryLiabilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RegulatoryLiabilityAxis_9ca85dda-916e-4d77-a70e-bdb10ef85001" xlink:to="loc_us-gaap_RegulatoryLiabilityDomain_85137647-87ff-4abd-964f-9fee10fd459e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_TaxRelatedMember_ca68a57f-7f52-47f8-a9bc-fcdca248fd97" xlink:href="lnt-20240331.xsd#lnt_TaxRelatedMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryLiabilityDomain_85137647-87ff-4abd-964f-9fee10fd459e" xlink:to="loc_lnt_TaxRelatedMember_ca68a57f-7f52-47f8-a9bc-fcdca248fd97" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RemovalCostsMember_88afb7de-0da0-48fb-83ef-42ffce663372" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RemovalCostsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryLiabilityDomain_85137647-87ff-4abd-964f-9fee10fd459e" xlink:to="loc_us-gaap_RemovalCostsMember_88afb7de-0da0-48fb-83ef-42ffce663372" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredDerivativeGainLossMember_72d06181-9947-4993-8766-281c6361b607" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DeferredDerivativeGainLossMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryLiabilityDomain_85137647-87ff-4abd-964f-9fee10fd459e" xlink:to="loc_us-gaap_DeferredDerivativeGainLossMember_72d06181-9947-4993-8766-281c6361b607" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_CommodityCostRecoveryMember_4387fdc3-bf21-44e2-93e4-97303cb3cfc1" xlink:href="lnt-20240331.xsd#lnt_CommodityCostRecoveryMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryLiabilityDomain_85137647-87ff-4abd-964f-9fee10fd459e" xlink:to="loc_lnt_CommodityCostRecoveryMember_4387fdc3-bf21-44e2-93e4-97303cb3cfc1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherRegulatoryAssetsLiabilitiesMember_61b80fd4-4e26-473d-b660-244c252df61c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherRegulatoryAssetsLiabilitiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryLiabilityDomain_85137647-87ff-4abd-964f-9fee10fd459e" xlink:to="loc_us-gaap_OtherRegulatoryAssetsLiabilitiesMember_61b80fd4-4e26-473d-b660-244c252df61c" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryLiabilities_ff88597b-5ea4-4cba-a76e-011ed5dbd00b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatoryLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryLiabilitiesLineItems_7f7d754a-8f46-4a24-8442-a4c93367ab95" xlink:to="loc_us-gaap_RegulatoryLiabilities_ff88597b-5ea4-4cba-a76e-011ed5dbd00b" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/PropertyPlantandEquipmentNarrativeDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#PropertyPlantandEquipmentNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/PropertyPlantandEquipmentNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentLineItems_52c2d74d-d698-485d-ac38-f59e1fdba90c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_fcc9a2af-741c-45b3-97a2-390e492dc841" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_52c2d74d-d698-485d-ac38-f59e1fdba90c" xlink:to="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_fcc9a2af-741c-45b3-97a2-390e492dc841" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementScenarioAxis_7533a03d-038c-48d1-9338-afbba912a258" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_StatementScenarioAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_fcc9a2af-741c-45b3-97a2-390e492dc841" xlink:to="loc_srt_StatementScenarioAxis_7533a03d-038c-48d1-9338-afbba912a258" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_7533a03d-038c-48d1-9338-afbba912a258_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementScenarioAxis_7533a03d-038c-48d1-9338-afbba912a258" xlink:to="loc_srt_ScenarioUnspecifiedDomain_7533a03d-038c-48d1-9338-afbba912a258_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_b25ddca0-c821-45fa-9de1-edf117a49b7c" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementScenarioAxis_7533a03d-038c-48d1-9338-afbba912a258" xlink:to="loc_srt_ScenarioUnspecifiedDomain_b25ddca0-c821-45fa-9de1-edf117a49b7c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PublicUtilityAxis_53c00491-634a-4a68-a45b-d06547bd6180" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PublicUtilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_fcc9a2af-741c-45b3-97a2-390e492dc841" xlink:to="loc_us-gaap_PublicUtilityAxis_53c00491-634a-4a68-a45b-d06547bd6180" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UtilityPlantDomain_53c00491-634a-4a68-a45b-d06547bd6180_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_UtilityPlantDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PublicUtilityAxis_53c00491-634a-4a68-a45b-d06547bd6180" xlink:to="loc_us-gaap_UtilityPlantDomain_53c00491-634a-4a68-a45b-d06547bd6180_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UtilityPlantDomain_121fcf4e-14d6-4e3b-afbd-acc322a6c866" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_UtilityPlantDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PublicUtilityAxis_53c00491-634a-4a68-a45b-d06547bd6180" xlink:to="loc_us-gaap_UtilityPlantDomain_121fcf4e-14d6-4e3b-afbd-acc322a6c866" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_76327bb2-825f-4f4f-8989-84c993f80354" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_fcc9a2af-741c-45b3-97a2-390e492dc841" xlink:to="loc_dei_LegalEntityAxis_76327bb2-825f-4f4f-8989-84c993f80354" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_76327bb2-825f-4f4f-8989-84c993f80354_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_76327bb2-825f-4f4f-8989-84c993f80354" xlink:to="loc_dei_EntityDomain_76327bb2-825f-4f4f-8989-84c993f80354_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_ff0d2029-6f7c-44d2-a62d-093f6af5a233" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_76327bb2-825f-4f4f-8989-84c993f80354" xlink:to="loc_dei_EntityDomain_ff0d2029-6f7c-44d2-a62d-093f6af5a233" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_7cf9f141-cd72-4ff9-ab40-f3905655f38c" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_ff0d2029-6f7c-44d2-a62d-093f6af5a233" xlink:to="loc_lnt_IplMember_7cf9f141-cd72-4ff9-ab40-f3905655f38c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_c6c49563-04f0-44e6-9d8c-e53d2e3b4571" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_ff0d2029-6f7c-44d2-a62d-093f6af5a233" xlink:to="loc_lnt_WplMember_c6c49563-04f0-44e6-9d8c-e53d2e3b4571" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_JointlyOwnedUtilityPlantAxis_90fe9d09-8ed5-4058-900d-3f7b6734547d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_JointlyOwnedUtilityPlantAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_fcc9a2af-741c-45b3-97a2-390e492dc841" xlink:to="loc_us-gaap_JointlyOwnedUtilityPlantAxis_90fe9d09-8ed5-4058-900d-3f7b6734547d" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_JointlyOwnedUtilityPlantDomain_90fe9d09-8ed5-4058-900d-3f7b6734547d_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_JointlyOwnedUtilityPlantDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_JointlyOwnedUtilityPlantAxis_90fe9d09-8ed5-4058-900d-3f7b6734547d" xlink:to="loc_us-gaap_JointlyOwnedUtilityPlantDomain_90fe9d09-8ed5-4058-900d-3f7b6734547d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_JointlyOwnedUtilityPlantDomain_9b5879b7-5578-4e5e-b869-552e0a4c0eea" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_JointlyOwnedUtilityPlantDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_JointlyOwnedUtilityPlantAxis_90fe9d09-8ed5-4058-900d-3f7b6734547d" xlink:to="loc_us-gaap_JointlyOwnedUtilityPlantDomain_9b5879b7-5578-4e5e-b869-552e0a4c0eea" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_ElectricCapacityOfSolarProject_ea413aeb-fccc-4bcf-a73a-1c8135c1b40e" xlink:href="lnt-20240331.xsd#lnt_ElectricCapacityOfSolarProject"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_52c2d74d-d698-485d-ac38-f59e1fdba90c" xlink:to="loc_lnt_ElectricCapacityOfSolarProject_ea413aeb-fccc-4bcf-a73a-1c8135c1b40e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_ConstructionProjectCostTargetExceedance_c4331849-074f-476d-9b53-73db667f4fd2" xlink:href="lnt-20240331.xsd#lnt_ConstructionProjectCostTargetExceedance"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_52c2d74d-d698-485d-ac38-f59e1fdba90c" xlink:to="loc_lnt_ConstructionProjectCostTargetExceedance_c4331849-074f-476d-9b53-73db667f4fd2" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/PropertyPlantandEquipmentEstimatedFairValueofAssetsPurchasedandLiabilitiesAssumedDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#PropertyPlantandEquipmentEstimatedFairValueofAssetsPurchasedandLiabilitiesAssumedDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/PropertyPlantandEquipmentEstimatedFairValueofAssetsPurchasedandLiabilitiesAssumedDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentLineItems_ac5c2634-1ad2-41b8-9611-fef974da9cbd" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_a67b32c4-51a7-43bc-9300-aa5a48e4562c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_ac5c2634-1ad2-41b8-9611-fef974da9cbd" xlink:to="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_a67b32c4-51a7-43bc-9300-aa5a48e4562c" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PublicUtilityAxis_0a07281b-1e6c-4830-9c1b-3662caca207a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PublicUtilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_a67b32c4-51a7-43bc-9300-aa5a48e4562c" xlink:to="loc_us-gaap_PublicUtilityAxis_0a07281b-1e6c-4830-9c1b-3662caca207a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UtilityPlantDomain_0a07281b-1e6c-4830-9c1b-3662caca207a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_UtilityPlantDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PublicUtilityAxis_0a07281b-1e6c-4830-9c1b-3662caca207a" xlink:to="loc_us-gaap_UtilityPlantDomain_0a07281b-1e6c-4830-9c1b-3662caca207a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UtilityPlantDomain_f259eddb-3baf-4781-bd93-6ea6a47afc44" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_UtilityPlantDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PublicUtilityAxis_0a07281b-1e6c-4830-9c1b-3662caca207a" xlink:to="loc_us-gaap_UtilityPlantDomain_f259eddb-3baf-4781-bd93-6ea6a47afc44" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_301537ff-b774-4afc-bc76-aff6db121b5f" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_a67b32c4-51a7-43bc-9300-aa5a48e4562c" xlink:to="loc_dei_LegalEntityAxis_301537ff-b774-4afc-bc76-aff6db121b5f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_301537ff-b774-4afc-bc76-aff6db121b5f_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_301537ff-b774-4afc-bc76-aff6db121b5f" xlink:to="loc_dei_EntityDomain_301537ff-b774-4afc-bc76-aff6db121b5f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_96c9525e-8808-452b-b1a2-ade71730c18b" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_301537ff-b774-4afc-bc76-aff6db121b5f" xlink:to="loc_dei_EntityDomain_96c9525e-8808-452b-b1a2-ade71730c18b" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/ReceivablesNarrativeDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#ReceivablesNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/ReceivablesNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_59094ffd-6f89-4478-bcd6-9524b227e9c3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccountsNotesAndLoansReceivableLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_c2173819-9733-4a98-83db-7ea858c89767" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_59094ffd-6f89-4478-bcd6-9524b227e9c3" xlink:to="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_c2173819-9733-4a98-83db-7ea858c89767" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsNotesLoansAndFinancingReceivableByReceivableTypeAxis_96242739-8ace-438c-b869-f4a834ad101a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccountsNotesLoansAndFinancingReceivableByReceivableTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_c2173819-9733-4a98-83db-7ea858c89767" xlink:to="loc_us-gaap_AccountsNotesLoansAndFinancingReceivableByReceivableTypeAxis_96242739-8ace-438c-b869-f4a834ad101a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReceivableTypeDomain_96242739-8ace-438c-b869-f4a834ad101a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ReceivableTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AccountsNotesLoansAndFinancingReceivableByReceivableTypeAxis_96242739-8ace-438c-b869-f4a834ad101a" xlink:to="loc_us-gaap_ReceivableTypeDomain_96242739-8ace-438c-b869-f4a834ad101a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReceivableTypeDomain_6c4d8270-7da5-4cf1-9e3b-3b1d479f9e27" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ReceivableTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AccountsNotesLoansAndFinancingReceivableByReceivableTypeAxis_96242739-8ace-438c-b869-f4a834ad101a" xlink:to="loc_us-gaap_ReceivableTypeDomain_6c4d8270-7da5-4cf1-9e3b-3b1d479f9e27" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinanceReceivablesMember_65af9a9b-6d8f-43dc-83c3-f0b00aba5baf" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FinanceReceivablesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReceivableTypeDomain_6c4d8270-7da5-4cf1-9e3b-3b1d479f9e27" xlink:to="loc_us-gaap_FinanceReceivablesMember_65af9a9b-6d8f-43dc-83c3-f0b00aba5baf" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_525b07b3-30ad-4d1e-92e7-260b4000178c" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_c2173819-9733-4a98-83db-7ea858c89767" xlink:to="loc_dei_LegalEntityAxis_525b07b3-30ad-4d1e-92e7-260b4000178c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_525b07b3-30ad-4d1e-92e7-260b4000178c_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_525b07b3-30ad-4d1e-92e7-260b4000178c" xlink:to="loc_dei_EntityDomain_525b07b3-30ad-4d1e-92e7-260b4000178c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_6700ea27-ba91-4990-a622-12e5afa5f8ab" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_525b07b3-30ad-4d1e-92e7-260b4000178c" xlink:to="loc_dei_EntityDomain_6700ea27-ba91-4990-a622-12e5afa5f8ab" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_d31fba17-46ce-415e-a117-a3feb9f0d9ae" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_6700ea27-ba91-4990-a622-12e5afa5f8ab" xlink:to="loc_lnt_IplMember_d31fba17-46ce-415e-a117-a3feb9f0d9ae" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_f4822ad0-51b9-40ec-a54b-420bb0e26507" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_6700ea27-ba91-4990-a622-12e5afa5f8ab" xlink:to="loc_lnt_WplMember_f4822ad0-51b9-40ec-a54b-420bb0e26507" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablesPeriodPastDueAxis_4282ab0a-01fb-4365-8c74-2fe59033c298" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FinancingReceivablesPeriodPastDueAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_c2173819-9733-4a98-83db-7ea858c89767" xlink:to="loc_us-gaap_FinancingReceivablesPeriodPastDueAxis_4282ab0a-01fb-4365-8c74-2fe59033c298" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablesPeriodPastDueDomain_4282ab0a-01fb-4365-8c74-2fe59033c298_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FinancingReceivablesPeriodPastDueDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivablesPeriodPastDueAxis_4282ab0a-01fb-4365-8c74-2fe59033c298" xlink:to="loc_us-gaap_FinancingReceivablesPeriodPastDueDomain_4282ab0a-01fb-4365-8c74-2fe59033c298_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablesPeriodPastDueDomain_04e15d72-f14f-464b-b78a-14d2f46e539d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FinancingReceivablesPeriodPastDueDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivablesPeriodPastDueAxis_4282ab0a-01fb-4365-8c74-2fe59033c298" xlink:to="loc_us-gaap_FinancingReceivablesPeriodPastDueDomain_04e15d72-f14f-464b-b78a-14d2f46e539d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialAssetPastDueMember_934ce2d4-f74f-47ab-9ce4-e799d5588a09" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FinancialAssetPastDueMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablesPeriodPastDueDomain_04e15d72-f14f-464b-b78a-14d2f46e539d" xlink:to="loc_us-gaap_FinancialAssetPastDueMember_934ce2d4-f74f-47ab-9ce4-e799d5588a09" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeAxis_615e326c-3423-4c63-a6f4-6ad48895019c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsequentEventTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_c2173819-9733-4a98-83db-7ea858c89767" xlink:to="loc_us-gaap_SubsequentEventTypeAxis_615e326c-3423-4c63-a6f4-6ad48895019c" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_615e326c-3423-4c63-a6f4-6ad48895019c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_615e326c-3423-4c63-a6f4-6ad48895019c" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_615e326c-3423-4c63-a6f4-6ad48895019c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_550494f8-fe70-48b3-a245-3efa5ebd52e1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_615e326c-3423-4c63-a6f4-6ad48895019c" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_550494f8-fe70-48b3-a245-3efa5ebd52e1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventMember_8d546872-86c6-42d5-9135-bac6f8fc4aa4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsequentEventMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventTypeDomain_550494f8-fe70-48b3-a245-3efa5ebd52e1" xlink:to="loc_us-gaap_SubsequentEventMember_8d546872-86c6-42d5-9135-bac6f8fc4aa4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_SaleofAccountsReceivableProgramCapacityAvailable_c11614e4-6040-457d-a795-9c8803d86aba" xlink:href="lnt-20240331.xsd#lnt_SaleofAccountsReceivableProgramCapacityAvailable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_59094ffd-6f89-4478-bcd6-9524b227e9c3" xlink:to="loc_lnt_SaleofAccountsReceivableProgramCapacityAvailable_c11614e4-6040-457d-a795-9c8803d86aba" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NotesReceivableGross_d931b8cb-9b50-4d98-b3ab-981adb265f9c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NotesReceivableGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_59094ffd-6f89-4478-bcd6-9524b227e9c3" xlink:to="loc_us-gaap_NotesReceivableGross_d931b8cb-9b50-4d98-b3ab-981adb265f9c" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/ReceivablesDetailsofAccountsReceivableDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#ReceivablesDetailsofAccountsReceivableDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/ReceivablesDetailsofAccountsReceivableDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_449d142b-9b01-43fd-8236-b8355c0175e4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccountsNotesAndLoansReceivableLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_61e0d1f9-e770-463b-97a5-9c207f05ef75" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_449d142b-9b01-43fd-8236-b8355c0175e4" xlink:to="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_61e0d1f9-e770-463b-97a5-9c207f05ef75" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_51915d2f-d427-4ae2-9688-1587b703bc3b" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_61e0d1f9-e770-463b-97a5-9c207f05ef75" xlink:to="loc_dei_LegalEntityAxis_51915d2f-d427-4ae2-9688-1587b703bc3b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_51915d2f-d427-4ae2-9688-1587b703bc3b_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_51915d2f-d427-4ae2-9688-1587b703bc3b" xlink:to="loc_dei_EntityDomain_51915d2f-d427-4ae2-9688-1587b703bc3b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_c77af9c4-973c-48eb-871a-6b25fd506eeb" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_51915d2f-d427-4ae2-9688-1587b703bc3b" xlink:to="loc_dei_EntityDomain_c77af9c4-973c-48eb-871a-6b25fd506eeb" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/ReceivablesGrossWriteoffsForAccountsReceivableDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#ReceivablesGrossWriteoffsForAccountsReceivableDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/ReceivablesGrossWriteoffsForAccountsReceivableDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_52b47627-2418-4b86-95a2-ffb6dc95d31a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FinancingReceivableRecordedInvestmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_4f7cdd31-1e09-4796-a731-562b6c9768f8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_52b47627-2418-4b86-95a2-ffb6dc95d31a" xlink:to="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_4f7cdd31-1e09-4796-a731-562b6c9768f8" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_4cc22a3b-d4c6-45ae-84e1-28222bc5358e" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_4f7cdd31-1e09-4796-a731-562b6c9768f8" xlink:to="loc_dei_LegalEntityAxis_4cc22a3b-d4c6-45ae-84e1-28222bc5358e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_4cc22a3b-d4c6-45ae-84e1-28222bc5358e_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_4cc22a3b-d4c6-45ae-84e1-28222bc5358e" xlink:to="loc_dei_EntityDomain_4cc22a3b-d4c6-45ae-84e1-28222bc5358e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_fbf30a76-0666-486f-8808-485f199fcdbc" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_4cc22a3b-d4c6-45ae-84e1-28222bc5358e" xlink:to="loc_dei_EntityDomain_fbf30a76-0666-486f-8808-485f199fcdbc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_3c9982d2-27c7-4542-8a90-20ccbacf11a3" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_fbf30a76-0666-486f-8808-485f199fcdbc" xlink:to="loc_lnt_IplMember_3c9982d2-27c7-4542-8a90-20ccbacf11a3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_70ded047-b2b1-45fc-b85c-056fdaf2f113" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_fbf30a76-0666-486f-8808-485f199fcdbc" xlink:to="loc_lnt_WplMember_70ded047-b2b1-45fc-b85c-056fdaf2f113" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/ReceivablesMaximumAndAverageOutstandingCashProceedsDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#ReceivablesMaximumAndAverageOutstandingCashProceedsDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/ReceivablesMaximumAndAverageOutstandingCashProceedsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_40fb07b4-8bba-4f3e-8f80-06724329d71f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccountsNotesAndLoansReceivableLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_f956bfbd-e791-470f-9487-cfc347c05c59" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_40fb07b4-8bba-4f3e-8f80-06724329d71f" xlink:to="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_f956bfbd-e791-470f-9487-cfc347c05c59" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_f3e0fd7c-7c78-42f8-83cd-e1ec698921d5" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_f956bfbd-e791-470f-9487-cfc347c05c59" xlink:to="loc_srt_RangeAxis_f3e0fd7c-7c78-42f8-83cd-e1ec698921d5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_f3e0fd7c-7c78-42f8-83cd-e1ec698921d5_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_f3e0fd7c-7c78-42f8-83cd-e1ec698921d5" xlink:to="loc_srt_RangeMember_f3e0fd7c-7c78-42f8-83cd-e1ec698921d5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_2578f3ac-fb85-4f6a-8f0a-e445b3dc05df" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_f3e0fd7c-7c78-42f8-83cd-e1ec698921d5" xlink:to="loc_srt_RangeMember_2578f3ac-fb85-4f6a-8f0a-e445b3dc05df" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_a1ddfaf8-6675-41dd-be49-445336184f16" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_2578f3ac-fb85-4f6a-8f0a-e445b3dc05df" xlink:to="loc_srt_MaximumMember_a1ddfaf8-6675-41dd-be49-445336184f16" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_WeightedAverageMember_8a367d73-4898-43f6-950a-814f642471a6" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_WeightedAverageMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_2578f3ac-fb85-4f6a-8f0a-e445b3dc05df" xlink:to="loc_srt_WeightedAverageMember_8a367d73-4898-43f6-950a-814f642471a6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_3bc7bf7f-2f01-4d88-8175-bff5b90bf92a" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_f956bfbd-e791-470f-9487-cfc347c05c59" xlink:to="loc_dei_LegalEntityAxis_3bc7bf7f-2f01-4d88-8175-bff5b90bf92a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_3bc7bf7f-2f01-4d88-8175-bff5b90bf92a_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_3bc7bf7f-2f01-4d88-8175-bff5b90bf92a" xlink:to="loc_dei_EntityDomain_3bc7bf7f-2f01-4d88-8175-bff5b90bf92a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_7c088447-62f3-4d33-9a5d-1ebfa7eebea2" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_3bc7bf7f-2f01-4d88-8175-bff5b90bf92a" xlink:to="loc_dei_EntityDomain_7c088447-62f3-4d33-9a5d-1ebfa7eebea2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_3fdb777f-af82-4354-874a-ed9a51faf87e" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_7c088447-62f3-4d33-9a5d-1ebfa7eebea2" xlink:to="loc_lnt_IplMember_3fdb777f-af82-4354-874a-ed9a51faf87e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromSaleAndCollectionOfReceivables_000cc011-72ce-49e0-a77f-4724305a61de" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ProceedsFromSaleAndCollectionOfReceivables"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_40fb07b4-8bba-4f3e-8f80-06724329d71f" xlink:to="loc_us-gaap_ProceedsFromSaleAndCollectionOfReceivables_000cc011-72ce-49e0-a77f-4724305a61de" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_AverageProceedsFromSaleAndCollectionOfReceivables_aecf9008-2d45-4f61-8ca0-5f580440b16f" xlink:href="lnt-20240331.xsd#lnt_AverageProceedsFromSaleAndCollectionOfReceivables"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_40fb07b4-8bba-4f3e-8f80-06724329d71f" xlink:to="loc_lnt_AverageProceedsFromSaleAndCollectionOfReceivables_aecf9008-2d45-4f61-8ca0-5f580440b16f" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/ReceivablesReceivablesSoldUnderTheAgreementDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#ReceivablesReceivablesSoldUnderTheAgreementDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/ReceivablesReceivablesSoldUnderTheAgreementDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d0cfd423-86cb-4e8f-b403-ae2e010beff7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccountsNotesAndLoansReceivableLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_cdd7586c-1cee-4d02-bb4b-46a825def5a6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d0cfd423-86cb-4e8f-b403-ae2e010beff7" xlink:to="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_cdd7586c-1cee-4d02-bb4b-46a825def5a6" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsNotesLoansAndFinancingReceivableByReceivableTypeAxis_18335054-1aff-4a96-a5ed-2e257e1b6672" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccountsNotesLoansAndFinancingReceivableByReceivableTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_cdd7586c-1cee-4d02-bb4b-46a825def5a6" xlink:to="loc_us-gaap_AccountsNotesLoansAndFinancingReceivableByReceivableTypeAxis_18335054-1aff-4a96-a5ed-2e257e1b6672" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReceivableTypeDomain_18335054-1aff-4a96-a5ed-2e257e1b6672_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ReceivableTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AccountsNotesLoansAndFinancingReceivableByReceivableTypeAxis_18335054-1aff-4a96-a5ed-2e257e1b6672" xlink:to="loc_us-gaap_ReceivableTypeDomain_18335054-1aff-4a96-a5ed-2e257e1b6672_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReceivableTypeDomain_80c30296-4d05-444e-a480-6913c4fa0e61" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ReceivableTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AccountsNotesLoansAndFinancingReceivableByReceivableTypeAxis_18335054-1aff-4a96-a5ed-2e257e1b6672" xlink:to="loc_us-gaap_ReceivableTypeDomain_80c30296-4d05-444e-a480-6913c4fa0e61" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinanceReceivablesMember_9acf2ff1-4e92-4c05-a5bb-3881be74232a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FinanceReceivablesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReceivableTypeDomain_80c30296-4d05-444e-a480-6913c4fa0e61" xlink:to="loc_us-gaap_FinanceReceivablesMember_9acf2ff1-4e92-4c05-a5bb-3881be74232a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_86551c8a-7046-4eec-bfb1-00beb5737bf5" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_cdd7586c-1cee-4d02-bb4b-46a825def5a6" xlink:to="loc_dei_LegalEntityAxis_86551c8a-7046-4eec-bfb1-00beb5737bf5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_86551c8a-7046-4eec-bfb1-00beb5737bf5_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_86551c8a-7046-4eec-bfb1-00beb5737bf5" xlink:to="loc_dei_EntityDomain_86551c8a-7046-4eec-bfb1-00beb5737bf5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_94167bc1-9385-4735-a715-bbd96f58cf4d" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_86551c8a-7046-4eec-bfb1-00beb5737bf5" xlink:to="loc_dei_EntityDomain_94167bc1-9385-4735-a715-bbd96f58cf4d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_9a35a6fa-7e92-4e6a-886b-aaee8117e74c" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_94167bc1-9385-4735-a715-bbd96f58cf4d" xlink:to="loc_lnt_IplMember_9a35a6fa-7e92-4e6a-886b-aaee8117e74c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_CustomerAccountsReceivableSoldAmount_e6279704-1ef0-44db-97a5-14fe631ad96c" xlink:href="lnt-20240331.xsd#lnt_CustomerAccountsReceivableSoldAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d0cfd423-86cb-4e8f-b403-ae2e010beff7" xlink:to="loc_lnt_CustomerAccountsReceivableSoldAmount_e6279704-1ef0-44db-97a5-14fe631ad96c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_UnbilledUtilityRevenuesSoldAmount_70dc97d2-1b64-477d-8738-cd145f147c54" xlink:href="lnt-20240331.xsd#lnt_UnbilledUtilityRevenuesSoldAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d0cfd423-86cb-4e8f-b403-ae2e010beff7" xlink:to="loc_lnt_UnbilledUtilityRevenuesSoldAmount_70dc97d2-1b64-477d-8738-cd145f147c54" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_OtherReceivablesSoldAmount_75951614-818b-4c63-b3a5-91d6c23328e5" xlink:href="lnt-20240331.xsd#lnt_OtherReceivablesSoldAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d0cfd423-86cb-4e8f-b403-ae2e010beff7" xlink:to="loc_lnt_OtherReceivablesSoldAmount_75951614-818b-4c63-b3a5-91d6c23328e5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_ReceivablesSold_e555609c-eaea-4d05-91ab-3a72b6e574c8" xlink:href="lnt-20240331.xsd#lnt_ReceivablesSold"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d0cfd423-86cb-4e8f-b403-ae2e010beff7" xlink:to="loc_lnt_ReceivablesSold_e555609c-eaea-4d05-91ab-3a72b6e574c8" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_CashProceedsFromReceivablesSold_13e913ba-fc7c-4cd8-906b-6c15081e74d3" xlink:href="lnt-20240331.xsd#lnt_CashProceedsFromReceivablesSold"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d0cfd423-86cb-4e8f-b403-ae2e010beff7" xlink:to="loc_lnt_CashProceedsFromReceivablesSold_13e913ba-fc7c-4cd8-906b-6c15081e74d3" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_DeferredProceedsOfReceivablesSold_0b4bec3d-405a-4802-a8b5-9ad71d7744d9" xlink:href="lnt-20240331.xsd#lnt_DeferredProceedsOfReceivablesSold"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d0cfd423-86cb-4e8f-b403-ae2e010beff7" xlink:to="loc_lnt_DeferredProceedsOfReceivablesSold_0b4bec3d-405a-4802-a8b5-9ad71d7744d9" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_AllowanceForDoubtfulAccountsReceivableSold_eaa3ffe2-c8ac-4084-bc28-fef270262b4e" xlink:href="lnt-20240331.xsd#lnt_AllowanceForDoubtfulAccountsReceivableSold"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d0cfd423-86cb-4e8f-b403-ae2e010beff7" xlink:to="loc_lnt_AllowanceForDoubtfulAccountsReceivableSold_eaa3ffe2-c8ac-4084-bc28-fef270262b4e" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_FairValueOfDeferredProceedsOfReceivablesSold_21590107-9c80-4607-bf48-517d221634e6" xlink:href="lnt-20240331.xsd#lnt_FairValueOfDeferredProceedsOfReceivablesSold"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d0cfd423-86cb-4e8f-b403-ae2e010beff7" xlink:to="loc_lnt_FairValueOfDeferredProceedsOfReceivablesSold_21590107-9c80-4607-bf48-517d221634e6" xlink:type="arc" order="7"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/ReceivablesAdditionalAttributesOfReceivablesSoldUnderTheAgreementDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#ReceivablesAdditionalAttributesOfReceivablesSoldUnderTheAgreementDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/ReceivablesAdditionalAttributesOfReceivablesSoldUnderTheAgreementDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_c749f04d-e776-427a-bd0e-4f66b3e480d9" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccountsNotesAndLoansReceivableLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_9ad6d45a-da00-4d2d-8ecb-67455a03071c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_c749f04d-e776-427a-bd0e-4f66b3e480d9" xlink:to="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_9ad6d45a-da00-4d2d-8ecb-67455a03071c" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_646ea25e-a0a1-48f8-8c86-1ea5aa21aa31" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_9ad6d45a-da00-4d2d-8ecb-67455a03071c" xlink:to="loc_dei_LegalEntityAxis_646ea25e-a0a1-48f8-8c86-1ea5aa21aa31" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_646ea25e-a0a1-48f8-8c86-1ea5aa21aa31_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_646ea25e-a0a1-48f8-8c86-1ea5aa21aa31" xlink:to="loc_dei_EntityDomain_646ea25e-a0a1-48f8-8c86-1ea5aa21aa31_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_8fab32f2-35cc-46bd-9672-f7353cc1cbdd" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_646ea25e-a0a1-48f8-8c86-1ea5aa21aa31" xlink:to="loc_dei_EntityDomain_8fab32f2-35cc-46bd-9672-f7353cc1cbdd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_914202b9-102e-4b80-a197-bb55688e55c5" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_8fab32f2-35cc-46bd-9672-f7353cc1cbdd" xlink:to="loc_lnt_IplMember_914202b9-102e-4b80-a197-bb55688e55c5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_CollectionsReinvestedInReceivables_1dd5670b-5679-4869-a487-d766b1ee1a8c" xlink:href="lnt-20240331.xsd#lnt_CollectionsReinvestedInReceivables"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_c749f04d-e776-427a-bd0e-4f66b3e480d9" xlink:to="loc_lnt_CollectionsReinvestedInReceivables_1dd5670b-5679-4869-a487-d766b1ee1a8c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_CreditLossesNetOfRecoveries_2addf22c-a0ef-41b0-8e5d-609fa03a0d91" xlink:href="lnt-20240331.xsd#lnt_CreditLossesNetOfRecoveries"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_c749f04d-e776-427a-bd0e-4f66b3e480d9" xlink:to="loc_lnt_CreditLossesNetOfRecoveries_2addf22c-a0ef-41b0-8e5d-609fa03a0d91" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/InvestmentsNarrativeDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#InvestmentsNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/InvestmentsNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_dc7fd52c-de88-418f-b2d1-9164dc1d8a23" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfEquityMethodInvestmentsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfEquityMethodInvestmentsTable_59b9b8f1-afda-4dd5-b6c0-8296b04d77c5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfEquityMethodInvestmentsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_dc7fd52c-de88-418f-b2d1-9164dc1d8a23" xlink:to="loc_us-gaap_ScheduleOfEquityMethodInvestmentsTable_59b9b8f1-afda-4dd5-b6c0-8296b04d77c5" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisposalGroupClassificationAxis_532c4f0e-71a2-48bb-96e7-e804c2e60949" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DisposalGroupClassificationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfEquityMethodInvestmentsTable_59b9b8f1-afda-4dd5-b6c0-8296b04d77c5" xlink:to="loc_us-gaap_DisposalGroupClassificationAxis_532c4f0e-71a2-48bb-96e7-e804c2e60949" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisposalGroupClassificationDomain_532c4f0e-71a2-48bb-96e7-e804c2e60949_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DisposalGroupClassificationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DisposalGroupClassificationAxis_532c4f0e-71a2-48bb-96e7-e804c2e60949" xlink:to="loc_us-gaap_DisposalGroupClassificationDomain_532c4f0e-71a2-48bb-96e7-e804c2e60949_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisposalGroupClassificationDomain_7b055072-4734-4a63-aa9f-638d11ccd302" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DisposalGroupClassificationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DisposalGroupClassificationAxis_532c4f0e-71a2-48bb-96e7-e804c2e60949" xlink:to="loc_us-gaap_DisposalGroupClassificationDomain_7b055072-4734-4a63-aa9f-638d11ccd302" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/InvestmentsUnconsolidatedEquityInvestmentsDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#InvestmentsUnconsolidatedEquityInvestmentsDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/InvestmentsUnconsolidatedEquityInvestmentsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_99be28eb-ed13-4fb4-9cb8-999bfea4f8e3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfEquityMethodInvestmentsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfEquityMethodInvestmentsTable_e6502b11-4317-4fc3-a1a6-b57749318c08" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfEquityMethodInvestmentsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_99be28eb-ed13-4fb4-9cb8-999bfea4f8e3" xlink:to="loc_us-gaap_ScheduleOfEquityMethodInvestmentsTable_e6502b11-4317-4fc3-a1a6-b57749318c08" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScheduleOfEquityMethodInvestmentEquityMethodInvesteeNameAxis_bbf46ef4-1153-4ccc-ad03-ddbf5465836a" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ScheduleOfEquityMethodInvestmentEquityMethodInvesteeNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfEquityMethodInvestmentsTable_e6502b11-4317-4fc3-a1a6-b57749318c08" xlink:to="loc_srt_ScheduleOfEquityMethodInvestmentEquityMethodInvesteeNameAxis_bbf46ef4-1153-4ccc-ad03-ddbf5465836a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_EquityMethodInvesteeNameDomain_bbf46ef4-1153-4ccc-ad03-ddbf5465836a_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_EquityMethodInvesteeNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ScheduleOfEquityMethodInvestmentEquityMethodInvesteeNameAxis_bbf46ef4-1153-4ccc-ad03-ddbf5465836a" xlink:to="loc_srt_EquityMethodInvesteeNameDomain_bbf46ef4-1153-4ccc-ad03-ddbf5465836a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_EquityMethodInvesteeNameDomain_d2ffd582-d0f6-486d-8ff2-072c39d8ac9e" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_EquityMethodInvesteeNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ScheduleOfEquityMethodInvestmentEquityMethodInvesteeNameAxis_bbf46ef4-1153-4ccc-ad03-ddbf5465836a" xlink:to="loc_srt_EquityMethodInvesteeNameDomain_d2ffd582-d0f6-486d-8ff2-072c39d8ac9e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_InvestmentInAtcMember_95799cfb-4acb-4de2-bf6f-841d09fd5cf7" xlink:href="lnt-20240331.xsd#lnt_InvestmentInAtcMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_EquityMethodInvesteeNameDomain_d2ffd582-d0f6-486d-8ff2-072c39d8ac9e" xlink:to="loc_lnt_InvestmentInAtcMember_95799cfb-4acb-4de2-bf6f-841d09fd5cf7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_OtherEquityMethodInvestmentsMember_31dba413-cb67-4a63-ad25-5320c9856343" xlink:href="lnt-20240331.xsd#lnt_OtherEquityMethodInvestmentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_EquityMethodInvesteeNameDomain_d2ffd582-d0f6-486d-8ff2-072c39d8ac9e" xlink:to="loc_lnt_OtherEquityMethodInvestmentsMember_31dba413-cb67-4a63-ad25-5320c9856343" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_TotalsMember_46f0a59c-7cff-4a4f-8f2a-102328f5aa20" xlink:href="lnt-20240331.xsd#lnt_TotalsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_EquityMethodInvesteeNameDomain_d2ffd582-d0f6-486d-8ff2-072c39d8ac9e" xlink:to="loc_lnt_TotalsMember_46f0a59c-7cff-4a4f-8f2a-102328f5aa20" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromEquityMethodInvestments_28784159-5ec0-4e01-9339-e299402d1b37" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeLossFromEquityMethodInvestments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_99be28eb-ed13-4fb4-9cb8-999bfea4f8e3" xlink:to="loc_us-gaap_IncomeLossFromEquityMethodInvestments_28784159-5ec0-4e01-9339-e299402d1b37" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/CommonEquityNarrativeDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#CommonEquityNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/CommonEquityNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_lnt_CommonEquityLineItems_cdd9a819-eec8-4d9f-8d94-79f50cbd071a" xlink:href="lnt-20240331.xsd#lnt_CommonEquityLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_CommonEquityTable_44c6ddac-cf4b-4ca7-a637-ffc62f68b9f4" xlink:href="lnt-20240331.xsd#lnt_CommonEquityTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_lnt_CommonEquityLineItems_cdd9a819-eec8-4d9f-8d94-79f50cbd071a" xlink:to="loc_lnt_CommonEquityTable_44c6ddac-cf4b-4ca7-a637-ffc62f68b9f4" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsidiarySaleOfStockAxis_4837f20a-0f66-4c27-874f-c7016f576202" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsidiarySaleOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lnt_CommonEquityTable_44c6ddac-cf4b-4ca7-a637-ffc62f68b9f4" xlink:to="loc_us-gaap_SubsidiarySaleOfStockAxis_4837f20a-0f66-4c27-874f-c7016f576202" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_4837f20a-0f66-4c27-874f-c7016f576202_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_4837f20a-0f66-4c27-874f-c7016f576202" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_4837f20a-0f66-4c27-874f-c7016f576202_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_58b3cd31-501a-4e83-addc-a1c500d4f764" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_4837f20a-0f66-4c27-874f-c7016f576202" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_58b3cd31-501a-4e83-addc-a1c500d4f764" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_3131ebef-2b1d-4e71-b71a-7188f1d78ecc" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lnt_CommonEquityTable_44c6ddac-cf4b-4ca7-a637-ffc62f68b9f4" xlink:to="loc_dei_LegalEntityAxis_3131ebef-2b1d-4e71-b71a-7188f1d78ecc" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_3131ebef-2b1d-4e71-b71a-7188f1d78ecc_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_3131ebef-2b1d-4e71-b71a-7188f1d78ecc" xlink:to="loc_dei_EntityDomain_3131ebef-2b1d-4e71-b71a-7188f1d78ecc_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_30026530-6b44-498a-bde2-95a5b5c79c5e" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_3131ebef-2b1d-4e71-b71a-7188f1d78ecc" xlink:to="loc_dei_EntityDomain_30026530-6b44-498a-bde2-95a5b5c79c5e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementScenarioAxis_149c5034-d570-4154-9ac7-7912921cb852" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_StatementScenarioAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lnt_CommonEquityTable_44c6ddac-cf4b-4ca7-a637-ffc62f68b9f4" xlink:to="loc_srt_StatementScenarioAxis_149c5034-d570-4154-9ac7-7912921cb852" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_149c5034-d570-4154-9ac7-7912921cb852_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementScenarioAxis_149c5034-d570-4154-9ac7-7912921cb852" xlink:to="loc_srt_ScenarioUnspecifiedDomain_149c5034-d570-4154-9ac7-7912921cb852_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_3bedb433-c0fa-48c3-94af-dcedf777c779" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementScenarioAxis_149c5034-d570-4154-9ac7-7912921cb852" xlink:to="loc_srt_ScenarioUnspecifiedDomain_3bedb433-c0fa-48c3-94af-dcedf777c779" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioForecastMember_ab01a391-4b9e-4375-add5-0e34b58dc8d5" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ScenarioForecastMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ScenarioUnspecifiedDomain_3bedb433-c0fa-48c3-94af-dcedf777c779" xlink:to="loc_srt_ScenarioForecastMember_ab01a391-4b9e-4375-add5-0e34b58dc8d5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeAxis_d8a4918c-54f8-451f-add2-fd0ac8dfb646" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsequentEventTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lnt_CommonEquityTable_44c6ddac-cf4b-4ca7-a637-ffc62f68b9f4" xlink:to="loc_us-gaap_SubsequentEventTypeAxis_d8a4918c-54f8-451f-add2-fd0ac8dfb646" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_d8a4918c-54f8-451f-add2-fd0ac8dfb646_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_d8a4918c-54f8-451f-add2-fd0ac8dfb646" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_d8a4918c-54f8-451f-add2-fd0ac8dfb646_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_0748b0a1-2ce1-462c-9a4d-eb863231e20d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_d8a4918c-54f8-451f-add2-fd0ac8dfb646" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_0748b0a1-2ce1-462c-9a4d-eb863231e20d" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/CommonEquityCommonShareActivityDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#CommonEquityCommonShareActivityDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/CommonEquityCommonShareActivityDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_lnt_CommonEquityLineItems_68d9d21e-6f80-4a00-9387-62ea25d8b4a8" xlink:href="lnt-20240331.xsd#lnt_CommonEquityLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_CommonEquityTable_342207fc-6574-4059-b46e-eefe726fbcc0" xlink:href="lnt-20240331.xsd#lnt_CommonEquityTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_lnt_CommonEquityLineItems_68d9d21e-6f80-4a00-9387-62ea25d8b4a8" xlink:to="loc_lnt_CommonEquityTable_342207fc-6574-4059-b46e-eefe726fbcc0" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsidiarySaleOfStockAxis_ab498e5e-b9ca-4b50-928e-19fc4397403b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsidiarySaleOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lnt_CommonEquityTable_342207fc-6574-4059-b46e-eefe726fbcc0" xlink:to="loc_us-gaap_SubsidiarySaleOfStockAxis_ab498e5e-b9ca-4b50-928e-19fc4397403b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_ab498e5e-b9ca-4b50-928e-19fc4397403b_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_ab498e5e-b9ca-4b50-928e-19fc4397403b" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_ab498e5e-b9ca-4b50-928e-19fc4397403b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_c2ca7a1a-338d-4e3f-b362-800e03df14d4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_ab498e5e-b9ca-4b50-928e-19fc4397403b" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_c2ca7a1a-338d-4e3f-b362-800e03df14d4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_e8fe92f6-3d43-4366-91cb-ffb7b4371c8c" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lnt_CommonEquityTable_342207fc-6574-4059-b46e-eefe726fbcc0" xlink:to="loc_dei_LegalEntityAxis_e8fe92f6-3d43-4366-91cb-ffb7b4371c8c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_e8fe92f6-3d43-4366-91cb-ffb7b4371c8c_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_e8fe92f6-3d43-4366-91cb-ffb7b4371c8c" xlink:to="loc_dei_EntityDomain_e8fe92f6-3d43-4366-91cb-ffb7b4371c8c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_334fc100-3bfb-409e-9e7f-6b786c69c429" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_e8fe92f6-3d43-4366-91cb-ffb7b4371c8c" xlink:to="loc_dei_EntityDomain_334fc100-3bfb-409e-9e7f-6b786c69c429" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeAxis_8fe66695-0d77-414a-9a01-8611cd6c3c27" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsequentEventTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lnt_CommonEquityTable_342207fc-6574-4059-b46e-eefe726fbcc0" xlink:to="loc_us-gaap_SubsequentEventTypeAxis_8fe66695-0d77-414a-9a01-8611cd6c3c27" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_8fe66695-0d77-414a-9a01-8611cd6c3c27_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_8fe66695-0d77-414a-9a01-8611cd6c3c27" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_8fe66695-0d77-414a-9a01-8611cd6c3c27_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_236c6278-e93e-4c51-86da-c1f51cab9527" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_8fe66695-0d77-414a-9a01-8611cd6c3c27" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_236c6278-e93e-4c51-86da-c1f51cab9527" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventMember_cf652a2a-fc65-4df8-892a-c7f2d2388d5e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsequentEventMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventTypeDomain_236c6278-e93e-4c51-86da-c1f51cab9527" xlink:to="loc_us-gaap_SubsequentEventMember_cf652a2a-fc65-4df8-892a-c7f2d2388d5e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_CommonStockOustandingRollForward_52be302b-3280-4519-ae77-4d8569068e70" xlink:href="lnt-20240331.xsd#lnt_CommonStockOustandingRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lnt_CommonEquityLineItems_68d9d21e-6f80-4a00-9387-62ea25d8b4a8" xlink:to="loc_lnt_CommonStockOustandingRollForward_52be302b-3280-4519-ae77-4d8569068e70" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_64e17abe-4193-40cc-bb7e-0522883fe8a1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lnt_CommonStockOustandingRollForward_52be302b-3280-4519-ae77-4d8569068e70" xlink:to="loc_us-gaap_CommonStockSharesOutstanding_64e17abe-4193-40cc-bb7e-0522883fe8a1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesDividendReinvestmentPlan_7f988a87-daba-492b-bbb4-e82cd4ae961f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockIssuedDuringPeriodSharesDividendReinvestmentPlan"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lnt_CommonStockOustandingRollForward_52be302b-3280-4519-ae77-4d8569068e70" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesDividendReinvestmentPlan_7f988a87-daba-492b-bbb4-e82cd4ae961f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensation_34b887bf-7408-42ed-a306-12b9c209caf6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lnt_CommonStockOustandingRollForward_52be302b-3280-4519-ae77-4d8569068e70" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensation_34b887bf-7408-42ed-a306-12b9c209caf6" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_3b183928-d2c0-433a-965b-60f9d3e05936" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockSharesOutstanding"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/CommonEquityChangesinShareownersEquityDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#CommonEquityChangesinShareownersEquityDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/CommonEquityChangesinShareownersEquityDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfCapitalizationEquityLineItems_35420a35-4691-422f-b39b-c4e10eee35ea" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfCapitalizationEquityLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfCapitalizationEquityTable_6f28f2e2-eb18-4034-a395-f0e760fb6a11" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfCapitalizationEquityTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ScheduleOfCapitalizationEquityLineItems_35420a35-4691-422f-b39b-c4e10eee35ea" xlink:to="loc_us-gaap_ScheduleOfCapitalizationEquityTable_6f28f2e2-eb18-4034-a395-f0e760fb6a11" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsForNewAccountingPronouncementsAxis_a69f3df0-3d7d-4cdc-aa9a-d6013345c249" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AdjustmentsForNewAccountingPronouncementsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfCapitalizationEquityTable_6f28f2e2-eb18-4034-a395-f0e760fb6a11" xlink:to="loc_us-gaap_AdjustmentsForNewAccountingPronouncementsAxis_a69f3df0-3d7d-4cdc-aa9a-d6013345c249" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TypeOfAdoptionMember_a69f3df0-3d7d-4cdc-aa9a-d6013345c249_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TypeOfAdoptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AdjustmentsForNewAccountingPronouncementsAxis_a69f3df0-3d7d-4cdc-aa9a-d6013345c249" xlink:to="loc_us-gaap_TypeOfAdoptionMember_a69f3df0-3d7d-4cdc-aa9a-d6013345c249_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TypeOfAdoptionMember_b393d599-80dc-400b-af88-39dc65ee28f5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TypeOfAdoptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AdjustmentsForNewAccountingPronouncementsAxis_a69f3df0-3d7d-4cdc-aa9a-d6013345c249" xlink:to="loc_us-gaap_TypeOfAdoptionMember_b393d599-80dc-400b-af88-39dc65ee28f5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_d30c20cf-d87c-424f-aa51-41c36392c624" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfCapitalizationEquityTable_6f28f2e2-eb18-4034-a395-f0e760fb6a11" xlink:to="loc_dei_LegalEntityAxis_d30c20cf-d87c-424f-aa51-41c36392c624" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_d30c20cf-d87c-424f-aa51-41c36392c624_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_d30c20cf-d87c-424f-aa51-41c36392c624" xlink:to="loc_dei_EntityDomain_d30c20cf-d87c-424f-aa51-41c36392c624_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_50b9aeb7-3079-4407-b948-e71e50f0ae2b" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_d30c20cf-d87c-424f-aa51-41c36392c624" xlink:to="loc_dei_EntityDomain_50b9aeb7-3079-4407-b948-e71e50f0ae2b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_bb984662-6f5a-4d54-aac1-bc49aa5f6c5d" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_50b9aeb7-3079-4407-b948-e71e50f0ae2b" xlink:to="loc_lnt_IplMember_bb984662-6f5a-4d54-aac1-bc49aa5f6c5d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_ad1426c2-2ea5-4eba-a109-c20b10f2edb5" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_50b9aeb7-3079-4407-b948-e71e50f0ae2b" xlink:to="loc_lnt_WplMember_ad1426c2-2ea5-4eba-a109-c20b10f2edb5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_41dfa592-0656-4348-ac26-d59b935b3877" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfCapitalizationEquityTable_6f28f2e2-eb18-4034-a395-f0e760fb6a11" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_41dfa592-0656-4348-ac26-d59b935b3877" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_41dfa592-0656-4348-ac26-d59b935b3877_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_41dfa592-0656-4348-ac26-d59b935b3877" xlink:to="loc_us-gaap_EquityComponentDomain_41dfa592-0656-4348-ac26-d59b935b3877_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_2d58c853-541b-4d1e-9605-2b3750ac014c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_41dfa592-0656-4348-ac26-d59b935b3877" xlink:to="loc_us-gaap_EquityComponentDomain_2d58c853-541b-4d1e-9605-2b3750ac014c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_9e8de633-b26e-4a68-980e-25e293bcc47c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_2d58c853-541b-4d1e-9605-2b3750ac014c" xlink:to="loc_us-gaap_CommonStockMember_9e8de633-b26e-4a68-980e-25e293bcc47c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalMember_31c2435a-3cf5-4778-9978-870799354a01" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AdditionalPaidInCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_2d58c853-541b-4d1e-9605-2b3750ac014c" xlink:to="loc_us-gaap_AdditionalPaidInCapitalMember_31c2435a-3cf5-4778-9978-870799354a01" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsMember_65ffcb69-8e5b-407a-8c60-22ceec2e362f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RetainedEarningsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_2d58c853-541b-4d1e-9605-2b3750ac014c" xlink:to="loc_us-gaap_RetainedEarningsMember_65ffcb69-8e5b-407a-8c60-22ceec2e362f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_1f0f0b7e-f00c-4272-a8b5-9dfb54736a9b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_2d58c853-541b-4d1e-9605-2b3750ac014c" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_1f0f0b7e-f00c-4272-a8b5-9dfb54736a9b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredCompensationShareBasedPaymentsMember_af678467-f31b-4703-8ec6-df435e811d80" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DeferredCompensationShareBasedPaymentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_2d58c853-541b-4d1e-9605-2b3750ac014c" xlink:to="loc_us-gaap_DeferredCompensationShareBasedPaymentsMember_af678467-f31b-4703-8ec6-df435e811d80" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_71f599cc-c4ba-4ebc-be0b-d63ba2ea5d60" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfCapitalizationEquityLineItems_35420a35-4691-422f-b39b-c4e10eee35ea" xlink:to="loc_us-gaap_StockholdersEquity_71f599cc-c4ba-4ebc-be0b-d63ba2ea5d60" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_050a5507-c40a-4050-bbad-5367a8692010" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfCapitalizationEquityLineItems_35420a35-4691-422f-b39b-c4e10eee35ea" xlink:to="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_050a5507-c40a-4050-bbad-5367a8692010" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic_8fb3fe77-17c5-4666-afae-9e85a7bb63e8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfCapitalizationEquityLineItems_35420a35-4691-422f-b39b-c4e10eee35ea" xlink:to="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic_8fb3fe77-17c5-4666-afae-9e85a7bb63e8" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_b08d1b5f-0635-4605-b1af-416af0749694" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfCapitalizationEquityLineItems_35420a35-4691-422f-b39b-c4e10eee35ea" xlink:to="loc_us-gaap_NetIncomeLoss_b08d1b5f-0635-4605-b1af-416af0749694" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DividendsCommonStock_4c8245e9-aa32-43df-bfaf-9c45466a47d9" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DividendsCommonStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfCapitalizationEquityLineItems_35420a35-4691-422f-b39b-c4e10eee35ea" xlink:to="loc_us-gaap_DividendsCommonStock_4c8245e9-aa32-43df-bfaf-9c45466a47d9" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOfCommonStock_16ee3cb4-18ec-4793-b015-67f08b232c2f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ProceedsFromIssuanceOfCommonStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfCapitalizationEquityLineItems_35420a35-4691-422f-b39b-c4e10eee35ea" xlink:to="loc_us-gaap_ProceedsFromIssuanceOfCommonStock_16ee3cb4-18ec-4793-b015-67f08b232c2f" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityOther_3fe1dac8-0fcb-48b8-9f09-94cf0e0808ab" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockholdersEquityOther"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfCapitalizationEquityLineItems_35420a35-4691-422f-b39b-c4e10eee35ea" xlink:to="loc_us-gaap_StockholdersEquityOther_3fe1dac8-0fcb-48b8-9f09-94cf0e0808ab" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromContributionsFromParent_8f350875-ac45-4fe4-991b-68b5c950f68a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ProceedsFromContributionsFromParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfCapitalizationEquityLineItems_35420a35-4691-422f-b39b-c4e10eee35ea" xlink:to="loc_us-gaap_ProceedsFromContributionsFromParent_8f350875-ac45-4fe4-991b-68b5c950f68a" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_1370d178-0a68-4908-a522-63974a75e256" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfCapitalizationEquityLineItems_35420a35-4691-422f-b39b-c4e10eee35ea" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_1370d178-0a68-4908-a522-63974a75e256" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_a45e1a06-c7ab-483f-aa17-1f5ebf628611" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_1f91c545-9e7b-4e24-b412-6924efcb269a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockDividendsPerShareDeclared_2bdffaa4-dc87-4356-a35e-8e9397eed680" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockDividendsPerShareDeclared"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfCapitalizationEquityLineItems_35420a35-4691-422f-b39b-c4e10eee35ea" xlink:to="loc_us-gaap_CommonStockDividendsPerShareDeclared_2bdffaa4-dc87-4356-a35e-8e9397eed680" xlink:type="arc" order="11"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/DebtNarrativeDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#DebtNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/DebtNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_549c450c-d099-4405-89b8-2aad86ca4b18" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_89153b22-b490-4ca0-946a-47e7728b7aee" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_549c450c-d099-4405-89b8-2aad86ca4b18" xlink:to="loc_us-gaap_DebtInstrumentTable_89153b22-b490-4ca0-946a-47e7728b7aee" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_dbf18fc0-a3be-4eac-8919-84fe0da03c86" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_89153b22-b490-4ca0-946a-47e7728b7aee" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_dbf18fc0-a3be-4eac-8919-84fe0da03c86" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_dbf18fc0-a3be-4eac-8919-84fe0da03c86_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_dbf18fc0-a3be-4eac-8919-84fe0da03c86" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_dbf18fc0-a3be-4eac-8919-84fe0da03c86_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_8fe0ae61-d8a8-4fed-bed1-44cc2e3c3d05" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_dbf18fc0-a3be-4eac-8919-84fe0da03c86" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_8fe0ae61-d8a8-4fed-bed1-44cc2e3c3d05" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_TermLoanCreditAgreementMember_7279ac9f-c04d-4eda-a1b8-bd67a4572746" xlink:href="lnt-20240331.xsd#lnt_TermLoanCreditAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_8fe0ae61-d8a8-4fed-bed1-44cc2e3c3d05" xlink:to="loc_lnt_TermLoanCreditAgreementMember_7279ac9f-c04d-4eda-a1b8-bd67a4572746" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_DebenturesMember_f3345b26-24a4-442f-93ea-9eee29d3cf02" xlink:href="lnt-20240331.xsd#lnt_DebenturesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_8fe0ae61-d8a8-4fed-bed1-44cc2e3c3d05" xlink:to="loc_lnt_DebenturesMember_f3345b26-24a4-442f-93ea-9eee29d3cf02" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConvertibleDebtMember_a5a6f4f1-2a26-4d09-9b93-48405c23e10a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConvertibleDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_8fe0ae61-d8a8-4fed-bed1-44cc2e3c3d05" xlink:to="loc_us-gaap_ConvertibleDebtMember_a5a6f4f1-2a26-4d09-9b93-48405c23e10a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_808ca2e4-4624-4607-a2af-b31299fa1166" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_89153b22-b490-4ca0-946a-47e7728b7aee" xlink:to="loc_us-gaap_DebtInstrumentAxis_808ca2e4-4624-4607-a2af-b31299fa1166" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_808ca2e4-4624-4607-a2af-b31299fa1166_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_808ca2e4-4624-4607-a2af-b31299fa1166" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_808ca2e4-4624-4607-a2af-b31299fa1166_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_45d84977-42d3-40a2-8cc2-25eb586d3f64" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_808ca2e4-4624-4607-a2af-b31299fa1166" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_45d84977-42d3-40a2-8cc2-25eb586d3f64" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_TermLoanCreditAgreementThroughMarchTwoThousandTwentyFiveMember_14aa0174-f990-4bff-ac02-e416e1b9107f" xlink:href="lnt-20240331.xsd#lnt_TermLoanCreditAgreementThroughMarchTwoThousandTwentyFiveMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_45d84977-42d3-40a2-8cc2-25eb586d3f64" xlink:to="loc_lnt_TermLoanCreditAgreementThroughMarchTwoThousandTwentyFiveMember_14aa0174-f990-4bff-ac02-e416e1b9107f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_TermLoanCreditAgreementThroughMarchTwoThousandTwentyFourMember_0b4e1f07-a5c9-4dc2-8ab3-6de0afbdd01c" xlink:href="lnt-20240331.xsd#lnt_TermLoanCreditAgreementThroughMarchTwoThousandTwentyFourMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_45d84977-42d3-40a2-8cc2-25eb586d3f64" xlink:to="loc_lnt_TermLoanCreditAgreementThroughMarchTwoThousandTwentyFourMember_0b4e1f07-a5c9-4dc2-8ab3-6de0afbdd01c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_FivePointThreeSevenFivePercentDebentureDueTwoThousandThirtyFourMember_f0b2cd4a-97a4-4a54-b181-1d45dfaec828" xlink:href="lnt-20240331.xsd#lnt_FivePointThreeSevenFivePercentDebentureDueTwoThousandThirtyFourMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_45d84977-42d3-40a2-8cc2-25eb586d3f64" xlink:to="loc_lnt_FivePointThreeSevenFivePercentDebentureDueTwoThousandThirtyFourMember_f0b2cd4a-97a4-4a54-b181-1d45dfaec828" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_ThreePointEightSevenFivePercentSeniorNotesDueTwoThousandTwentySixMember_69068e1a-916e-4466-8383-ad0eb35af8ed" xlink:href="lnt-20240331.xsd#lnt_ThreePointEightSevenFivePercentSeniorNotesDueTwoThousandTwentySixMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_45d84977-42d3-40a2-8cc2-25eb586d3f64" xlink:to="loc_lnt_ThreePointEightSevenFivePercentSeniorNotesDueTwoThousandTwentySixMember_69068e1a-916e-4466-8383-ad0eb35af8ed" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_36288dd9-a1bc-460f-afbd-0a724b40b1bb" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_89153b22-b490-4ca0-946a-47e7728b7aee" xlink:to="loc_dei_LegalEntityAxis_36288dd9-a1bc-460f-afbd-0a724b40b1bb" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_36288dd9-a1bc-460f-afbd-0a724b40b1bb_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_36288dd9-a1bc-460f-afbd-0a724b40b1bb" xlink:to="loc_dei_EntityDomain_36288dd9-a1bc-460f-afbd-0a724b40b1bb_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_6d6668ec-2028-40b8-9e1c-3a3c8cfdac91" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_36288dd9-a1bc-460f-afbd-0a724b40b1bb" xlink:to="loc_dei_EntityDomain_6d6668ec-2028-40b8-9e1c-3a3c8cfdac91" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_5f5641d6-decc-4e58-8444-27f097e746cd" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_6d6668ec-2028-40b8-9e1c-3a3c8cfdac91" xlink:to="loc_lnt_IplMember_5f5641d6-decc-4e58-8444-27f097e746cd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_0f92eb10-b1a1-49ba-984b-ee302634a2fe" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_6d6668ec-2028-40b8-9e1c-3a3c8cfdac91" xlink:to="loc_lnt_WplMember_0f92eb10-b1a1-49ba-984b-ee302634a2fe" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_AlliantEnergyFinanceLLCMember_db9f1bf2-aefd-4b0f-bf1a-16a9b8d0303e" xlink:href="lnt-20240331.xsd#lnt_AlliantEnergyFinanceLLCMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_6d6668ec-2028-40b8-9e1c-3a3c8cfdac91" xlink:to="loc_lnt_AlliantEnergyFinanceLLCMember_db9f1bf2-aefd-4b0f-bf1a-16a9b8d0303e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidatedEntitiesAxis_4c2f4947-c854-43b2-8ca0-f65439a2e86a" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ConsolidatedEntitiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_89153b22-b490-4ca0-946a-47e7728b7aee" xlink:to="loc_srt_ConsolidatedEntitiesAxis_4c2f4947-c854-43b2-8ca0-f65439a2e86a" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidatedEntitiesDomain_4c2f4947-c854-43b2-8ca0-f65439a2e86a_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ConsolidatedEntitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ConsolidatedEntitiesAxis_4c2f4947-c854-43b2-8ca0-f65439a2e86a" xlink:to="loc_srt_ConsolidatedEntitiesDomain_4c2f4947-c854-43b2-8ca0-f65439a2e86a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidatedEntitiesDomain_56bade81-e472-4c5e-b086-1cad506190a7" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ConsolidatedEntitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ConsolidatedEntitiesAxis_4c2f4947-c854-43b2-8ca0-f65439a2e86a" xlink:to="loc_srt_ConsolidatedEntitiesDomain_56bade81-e472-4c5e-b086-1cad506190a7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ParentCompanyMember_38aa6a79-866b-415a-ac98-d50e4b9294f6" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ParentCompanyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidatedEntitiesDomain_56bade81-e472-4c5e-b086-1cad506190a7" xlink:to="loc_srt_ParentCompanyMember_38aa6a79-866b-415a-ac98-d50e4b9294f6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_cd0bd224-5a15-47d1-966c-312b18eee430" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_89153b22-b490-4ca0-946a-47e7728b7aee" xlink:to="loc_srt_RangeAxis_cd0bd224-5a15-47d1-966c-312b18eee430" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_cd0bd224-5a15-47d1-966c-312b18eee430_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_cd0bd224-5a15-47d1-966c-312b18eee430" xlink:to="loc_srt_RangeMember_cd0bd224-5a15-47d1-966c-312b18eee430_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_5451c588-528f-453e-96ea-84350e3b6dbc" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_cd0bd224-5a15-47d1-966c-312b18eee430" xlink:to="loc_srt_RangeMember_5451c588-528f-453e-96ea-84350e3b6dbc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_87f637e1-6630-4e75-89fa-e91d5d21d59f" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_5451c588-528f-453e-96ea-84350e3b6dbc" xlink:to="loc_srt_MaximumMember_87f637e1-6630-4e75-89fa-e91d5d21d59f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_d19f1b6c-ba54-4e72-bf7b-c9d1c988d067" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_5451c588-528f-453e-96ea-84350e3b6dbc" xlink:to="loc_srt_MinimumMember_d19f1b6c-ba54-4e72-bf7b-c9d1c988d067" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementScenarioAxis_0fe5d71a-9819-4c25-b233-32884b077814" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_StatementScenarioAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_89153b22-b490-4ca0-946a-47e7728b7aee" xlink:to="loc_srt_StatementScenarioAxis_0fe5d71a-9819-4c25-b233-32884b077814" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_0fe5d71a-9819-4c25-b233-32884b077814_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementScenarioAxis_0fe5d71a-9819-4c25-b233-32884b077814" xlink:to="loc_srt_ScenarioUnspecifiedDomain_0fe5d71a-9819-4c25-b233-32884b077814_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_cc872217-f5c5-452d-a24e-1c9d8e6cd701" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementScenarioAxis_0fe5d71a-9819-4c25-b233-32884b077814" xlink:to="loc_srt_ScenarioUnspecifiedDomain_cc872217-f5c5-452d-a24e-1c9d8e6cd701" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioForecastMember_3747c1d4-b41a-4a8e-931e-562e9eb38e8b" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ScenarioForecastMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ScenarioUnspecifiedDomain_cc872217-f5c5-452d-a24e-1c9d8e6cd701" xlink:to="loc_srt_ScenarioForecastMember_3747c1d4-b41a-4a8e-931e-562e9eb38e8b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityCurrentBorrowingCapacity_4b811c7d-ae83-4b4f-beaf-314c0d8f297b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditFacilityCurrentBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_549c450c-d099-4405-89b8-2aad86ca4b18" xlink:to="loc_us-gaap_LineOfCreditFacilityCurrentBorrowingCapacity_4b811c7d-ae83-4b4f-beaf-314c0d8f297b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentCarryingAmount_30575fe3-1194-4149-a209-4aecce39ee1d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentCarryingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_549c450c-d099-4405-89b8-2aad86ca4b18" xlink:to="loc_us-gaap_DebtInstrumentCarryingAmount_30575fe3-1194-4149-a209-4aecce39ee1d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_4297b4bf-9e87-4a7f-baa1-11079c3b0d14" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentInterestRateStatedPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_549c450c-d099-4405-89b8-2aad86ca4b18" xlink:to="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_4297b4bf-9e87-4a7f-baa1-11079c3b0d14" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RepaymentsOfLongTermDebt_d992b825-4366-4ce0-9043-6469f42a259c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RepaymentsOfLongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_549c450c-d099-4405-89b8-2aad86ca4b18" xlink:to="loc_us-gaap_RepaymentsOfLongTermDebt_d992b825-4366-4ce0-9043-6469f42a259c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_34d54997-b62f-4c66-9873-fd3d985ed457" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_549c450c-d099-4405-89b8-2aad86ca4b18" xlink:to="loc_us-gaap_LongTermDebt_34d54997-b62f-4c66-9873-fd3d985ed457" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnamortizedDebtIssuanceExpense_fde813ff-90b7-44c9-a26d-62ad85b53996" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_UnamortizedDebtIssuanceExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_549c450c-d099-4405-89b8-2aad86ca4b18" xlink:to="loc_us-gaap_UnamortizedDebtIssuanceExpense_fde813ff-90b7-44c9-a26d-62ad85b53996" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtFairValue_65043964-3d68-438d-a30d-5176d6e6940d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermDebtFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_549c450c-d099-4405-89b8-2aad86ca4b18" xlink:to="loc_us-gaap_LongTermDebtFairValue_65043964-3d68-438d-a30d-5176d6e6940d" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberDilutedSharesOutstandingAdjustment_90c91bcc-4949-4c7e-863c-9b11367eaeb5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_WeightedAverageNumberDilutedSharesOutstandingAdjustment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_549c450c-d099-4405-89b8-2aad86ca4b18" xlink:to="loc_us-gaap_WeightedAverageNumberDilutedSharesOutstandingAdjustment_90c91bcc-4949-4c7e-863c-9b11367eaeb5" xlink:type="arc" order="7"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/DebtCreditFacilitiesDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#DebtCreditFacilitiesDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/DebtCreditFacilitiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_fdd11e36-80c1-47df-b636-38362214d985" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_09f8a821-65cd-4121-827e-85c26c765990" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_fdd11e36-80c1-47df-b636-38362214d985" xlink:to="loc_us-gaap_DebtInstrumentTable_09f8a821-65cd-4121-827e-85c26c765990" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_3a644662-9f0d-4200-92f7-38973a42e3d4" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_09f8a821-65cd-4121-827e-85c26c765990" xlink:to="loc_dei_LegalEntityAxis_3a644662-9f0d-4200-92f7-38973a42e3d4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_3a644662-9f0d-4200-92f7-38973a42e3d4_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_3a644662-9f0d-4200-92f7-38973a42e3d4" xlink:to="loc_dei_EntityDomain_3a644662-9f0d-4200-92f7-38973a42e3d4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_a09fdcc9-2c1f-4d9d-bee1-d2b07c432a82" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_3a644662-9f0d-4200-92f7-38973a42e3d4" xlink:to="loc_dei_EntityDomain_a09fdcc9-2c1f-4d9d-bee1-d2b07c432a82" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_34cf3374-bed3-461a-b83c-f12d407d5312" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_a09fdcc9-2c1f-4d9d-bee1-d2b07c432a82" xlink:to="loc_lnt_IplMember_34cf3374-bed3-461a-b83c-f12d407d5312" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_0576d35f-67ad-4e1e-b5d1-da2da2419405" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_a09fdcc9-2c1f-4d9d-bee1-d2b07c432a82" xlink:to="loc_lnt_WplMember_0576d35f-67ad-4e1e-b5d1-da2da2419405" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShortTermBorrowings_ea53e8df-33f9-4179-b562-ebc9165998aa" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShortTermBorrowings"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_fdd11e36-80c1-47df-b636-38362214d985" xlink:to="loc_us-gaap_ShortTermBorrowings_ea53e8df-33f9-4179-b562-ebc9165998aa" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShortTermDebtWeightedAverageInterestRate_e825f139-132b-408d-8e46-50cec21a844d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShortTermDebtWeightedAverageInterestRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_fdd11e36-80c1-47df-b636-38362214d985" xlink:to="loc_us-gaap_ShortTermDebtWeightedAverageInterestRate_e825f139-132b-408d-8e46-50cec21a844d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityRemainingBorrowingCapacity_382f7cae-59ff-4b6b-9438-5abd8d757719" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditFacilityRemainingBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_fdd11e36-80c1-47df-b636-38362214d985" xlink:to="loc_us-gaap_LineOfCreditFacilityRemainingBorrowingCapacity_382f7cae-59ff-4b6b-9438-5abd8d757719" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/DebtOtherShortTermBorrowingsDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#DebtOtherShortTermBorrowingsDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/DebtOtherShortTermBorrowingsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_8d7a2698-9d4a-4043-afcc-d33a3d1dccb0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_0c2bf378-a171-488d-bbbf-620a3e236279" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_8d7a2698-9d4a-4043-afcc-d33a3d1dccb0" xlink:to="loc_us-gaap_DebtInstrumentTable_0c2bf378-a171-488d-bbbf-620a3e236279" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_5d3f6d3d-95a8-4f87-8602-b5e9a756d1b8" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_0c2bf378-a171-488d-bbbf-620a3e236279" xlink:to="loc_dei_LegalEntityAxis_5d3f6d3d-95a8-4f87-8602-b5e9a756d1b8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_5d3f6d3d-95a8-4f87-8602-b5e9a756d1b8_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_5d3f6d3d-95a8-4f87-8602-b5e9a756d1b8" xlink:to="loc_dei_EntityDomain_5d3f6d3d-95a8-4f87-8602-b5e9a756d1b8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_400ca722-1e52-455c-a870-51d726ec3c13" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_5d3f6d3d-95a8-4f87-8602-b5e9a756d1b8" xlink:to="loc_dei_EntityDomain_400ca722-1e52-455c-a870-51d726ec3c13" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_9c6b6c90-a857-47a6-902c-347e425b5d43" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_400ca722-1e52-455c-a870-51d726ec3c13" xlink:to="loc_lnt_IplMember_9c6b6c90-a857-47a6-902c-347e425b5d43" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_7cee3ee2-9542-4a5c-86b1-ca7365134c7c" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_400ca722-1e52-455c-a870-51d726ec3c13" xlink:to="loc_lnt_WplMember_7cee3ee2-9542-4a5c-86b1-ca7365134c7c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShorttermDebtMaximumAmountOutstandingDuringPeriod_46aeaa72-e1ef-4fad-bb24-60a639334aec" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShorttermDebtMaximumAmountOutstandingDuringPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_8d7a2698-9d4a-4043-afcc-d33a3d1dccb0" xlink:to="loc_us-gaap_ShorttermDebtMaximumAmountOutstandingDuringPeriod_46aeaa72-e1ef-4fad-bb24-60a639334aec" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShorttermDebtAverageOutstandingAmount_07488825-fadf-4a95-9ff9-db1ec09c9e55" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShorttermDebtAverageOutstandingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_8d7a2698-9d4a-4043-afcc-d33a3d1dccb0" xlink:to="loc_us-gaap_ShorttermDebtAverageOutstandingAmount_07488825-fadf-4a95-9ff9-db1ec09c9e55" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShortTermDebtWeightedAverageInterestRateOverTime_7bd63d7f-734b-45c9-845f-5aa1d60c3920" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShortTermDebtWeightedAverageInterestRateOverTime"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_8d7a2698-9d4a-4043-afcc-d33a3d1dccb0" xlink:to="loc_us-gaap_ShortTermDebtWeightedAverageInterestRateOverTime_7bd63d7f-734b-45c9-845f-5aa1d60c3920" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/RevenueDisaggregationofRevenuesDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#RevenueDisaggregationofRevenuesDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/RevenueDisaggregationofRevenuesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueLineItems_b7f844de-3a8f-4d7b-9d7c-12f4f63ecc47" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DisaggregationOfRevenueLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueTable_93a71390-d048-4f89-bf5b-9f8abfb1a15e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DisaggregationOfRevenueTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_b7f844de-3a8f-4d7b-9d7c-12f4f63ecc47" xlink:to="loc_us-gaap_DisaggregationOfRevenueTable_93a71390-d048-4f89-bf5b-9f8abfb1a15e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_e6fbd4cf-b6ff-470f-b8ab-9aeca99ae6b2" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_93a71390-d048-4f89-bf5b-9f8abfb1a15e" xlink:to="loc_dei_LegalEntityAxis_e6fbd4cf-b6ff-470f-b8ab-9aeca99ae6b2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_e6fbd4cf-b6ff-470f-b8ab-9aeca99ae6b2_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_e6fbd4cf-b6ff-470f-b8ab-9aeca99ae6b2" xlink:to="loc_dei_EntityDomain_e6fbd4cf-b6ff-470f-b8ab-9aeca99ae6b2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_51279824-5aad-43e3-98a5-8b4616c147fe" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_e6fbd4cf-b6ff-470f-b8ab-9aeca99ae6b2" xlink:to="loc_dei_EntityDomain_51279824-5aad-43e3-98a5-8b4616c147fe" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_d2338602-fbc3-4d76-9da9-b427f735d391" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_51279824-5aad-43e3-98a5-8b4616c147fe" xlink:to="loc_lnt_IplMember_d2338602-fbc3-4d76-9da9-b427f735d391" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_5dc404f2-5a1f-47a0-830b-1c9323d4e5e5" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_51279824-5aad-43e3-98a5-8b4616c147fe" xlink:to="loc_lnt_WplMember_5dc404f2-5a1f-47a0-830b-1c9323d4e5e5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_082901cd-4be2-4d74-868d-00cb0a50d74c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_93a71390-d048-4f89-bf5b-9f8abfb1a15e" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_082901cd-4be2-4d74-868d-00cb0a50d74c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_082901cd-4be2-4d74-868d-00cb0a50d74c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_082901cd-4be2-4d74-868d-00cb0a50d74c" xlink:to="loc_us-gaap_SegmentDomain_082901cd-4be2-4d74-868d-00cb0a50d74c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_c724ff3e-cb9f-4bc0-9f54-1b1b9f84fc76" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_082901cd-4be2-4d74-868d-00cb0a50d74c" xlink:to="loc_us-gaap_SegmentDomain_c724ff3e-cb9f-4bc0-9f54-1b1b9f84fc76" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_ElectricMember_8d145ae1-c2ce-48a1-9f86-8a6a71ad24ea" xlink:href="lnt-20240331.xsd#lnt_ElectricMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_c724ff3e-cb9f-4bc0-9f54-1b1b9f84fc76" xlink:to="loc_lnt_ElectricMember_8d145ae1-c2ce-48a1-9f86-8a6a71ad24ea" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_GasMember_ff3e7616-bf95-48a3-85ab-260d4095d3c1" xlink:href="lnt-20240331.xsd#lnt_GasMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_c724ff3e-cb9f-4bc0-9f54-1b1b9f84fc76" xlink:to="loc_lnt_GasMember_ff3e7616-bf95-48a3-85ab-260d4095d3c1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_OtherUtilityMember_4e658088-02a8-4499-8b58-5137c7697033" xlink:href="lnt-20240331.xsd#lnt_OtherUtilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_c724ff3e-cb9f-4bc0-9f54-1b1b9f84fc76" xlink:to="loc_lnt_OtherUtilityMember_4e658088-02a8-4499-8b58-5137c7697033" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllOtherSegmentsMember_1d0f6235-209e-49d7-b2d8-e1099fe9f82f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AllOtherSegmentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_c724ff3e-cb9f-4bc0-9f54-1b1b9f84fc76" xlink:to="loc_us-gaap_AllOtherSegmentsMember_1d0f6235-209e-49d7-b2d8-e1099fe9f82f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MajorCustomersAxis_94e40c77-1e0f-477c-a9e9-49cf41b4b421" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_MajorCustomersAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_93a71390-d048-4f89-bf5b-9f8abfb1a15e" xlink:to="loc_srt_MajorCustomersAxis_94e40c77-1e0f-477c-a9e9-49cf41b4b421" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_NameOfMajorCustomerDomain_94e40c77-1e0f-477c-a9e9-49cf41b4b421_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_NameOfMajorCustomerDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_MajorCustomersAxis_94e40c77-1e0f-477c-a9e9-49cf41b4b421" xlink:to="loc_srt_NameOfMajorCustomerDomain_94e40c77-1e0f-477c-a9e9-49cf41b4b421_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_NameOfMajorCustomerDomain_9149101a-f240-4212-8019-650644917730" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_NameOfMajorCustomerDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_MajorCustomersAxis_94e40c77-1e0f-477c-a9e9-49cf41b4b421" xlink:to="loc_srt_NameOfMajorCustomerDomain_9149101a-f240-4212-8019-650644917730" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_RetailResidentialMember_cd2bd5e9-9d5d-46d8-bcee-a9bf15099958" xlink:href="lnt-20240331.xsd#lnt_RetailResidentialMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_NameOfMajorCustomerDomain_9149101a-f240-4212-8019-650644917730" xlink:to="loc_lnt_RetailResidentialMember_cd2bd5e9-9d5d-46d8-bcee-a9bf15099958" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_RetailCommercialMember_3aed3029-486c-406f-b168-3e0dd09103a4" xlink:href="lnt-20240331.xsd#lnt_RetailCommercialMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_NameOfMajorCustomerDomain_9149101a-f240-4212-8019-650644917730" xlink:to="loc_lnt_RetailCommercialMember_3aed3029-486c-406f-b168-3e0dd09103a4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_RetailIndustrialMember_f577f174-b4c2-4337-88c4-d17a67423aa6" xlink:href="lnt-20240331.xsd#lnt_RetailIndustrialMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_NameOfMajorCustomerDomain_9149101a-f240-4212-8019-650644917730" xlink:to="loc_lnt_RetailIndustrialMember_f577f174-b4c2-4337-88c4-d17a67423aa6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WholesaleMember_7c1b0c3e-7aa2-4c06-ae66-27acdfaa1ca5" xlink:href="lnt-20240331.xsd#lnt_WholesaleMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_NameOfMajorCustomerDomain_9149101a-f240-4212-8019-650644917730" xlink:to="loc_lnt_WholesaleMember_7c1b0c3e-7aa2-4c06-ae66-27acdfaa1ca5" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_SteamMember_39a10f46-7661-418f-9749-3ce9bf1bd843" xlink:href="lnt-20240331.xsd#lnt_SteamMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_NameOfMajorCustomerDomain_9149101a-f240-4212-8019-650644917730" xlink:to="loc_lnt_SteamMember_39a10f46-7661-418f-9749-3ce9bf1bd843" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherCustomerMember_13943afb-84e3-4830-8b84-4c7ca4c6fc89" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherCustomerMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_NameOfMajorCustomerDomain_9149101a-f240-4212-8019-650644917730" xlink:to="loc_us-gaap_OtherCustomerMember_13943afb-84e3-4830-8b84-4c7ca4c6fc89" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_789707fa-1f5c-4b5d-ac86-e1052359cd01" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_b7f844de-3a8f-4d7b-9d7c-12f4f63ecc47" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_789707fa-1f5c-4b5d-ac86-e1052359cd01" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/IncomeTaxesNarrativeDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#IncomeTaxesNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/IncomeTaxesNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IncomeTaxLineItems_222cf004-2e4a-41f3-bbe9-788a3d4d51a6" xlink:href="lnt-20240331.xsd#lnt_IncomeTaxLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IncomeTaxTable_b322352b-ac26-4359-abaa-f2a53559d1c6" xlink:href="lnt-20240331.xsd#lnt_IncomeTaxTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_lnt_IncomeTaxLineItems_222cf004-2e4a-41f3-bbe9-788a3d4d51a6" xlink:to="loc_lnt_IncomeTaxTable_b322352b-ac26-4359-abaa-f2a53559d1c6" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_8fc23b3b-0299-458c-aaa3-3bcf24f8f1a2" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lnt_IncomeTaxTable_b322352b-ac26-4359-abaa-f2a53559d1c6" xlink:to="loc_dei_LegalEntityAxis_8fc23b3b-0299-458c-aaa3-3bcf24f8f1a2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_8fc23b3b-0299-458c-aaa3-3bcf24f8f1a2_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_8fc23b3b-0299-458c-aaa3-3bcf24f8f1a2" xlink:to="loc_dei_EntityDomain_8fc23b3b-0299-458c-aaa3-3bcf24f8f1a2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_d06eb620-2d39-46d1-8e84-af9073f19dc8" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_8fc23b3b-0299-458c-aaa3-3bcf24f8f1a2" xlink:to="loc_dei_EntityDomain_d06eb620-2d39-46d1-8e84-af9073f19dc8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_c0fbbbb5-44c9-4dfa-bf61-9fce1ed27ad8" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_d06eb620-2d39-46d1-8e84-af9073f19dc8" xlink:to="loc_lnt_IplMember_c0fbbbb5-44c9-4dfa-bf61-9fce1ed27ad8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_20c81073-983d-4640-9779-738a4c2c8421" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_d06eb620-2d39-46d1-8e84-af9073f19dc8" xlink:to="loc_lnt_WplMember_20c81073-983d-4640-9779-738a4c2c8421" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementScenarioAxis_b0267c6a-6bf7-4e51-9af1-c8c7a0c4d5d9" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_StatementScenarioAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lnt_IncomeTaxTable_b322352b-ac26-4359-abaa-f2a53559d1c6" xlink:to="loc_srt_StatementScenarioAxis_b0267c6a-6bf7-4e51-9af1-c8c7a0c4d5d9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_b0267c6a-6bf7-4e51-9af1-c8c7a0c4d5d9_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementScenarioAxis_b0267c6a-6bf7-4e51-9af1-c8c7a0c4d5d9" xlink:to="loc_srt_ScenarioUnspecifiedDomain_b0267c6a-6bf7-4e51-9af1-c8c7a0c4d5d9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_ee9a14a6-a53e-4f1b-8db2-311cd14d15d0" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementScenarioAxis_b0267c6a-6bf7-4e51-9af1-c8c7a0c4d5d9" xlink:to="loc_srt_ScenarioUnspecifiedDomain_ee9a14a6-a53e-4f1b-8db2-311cd14d15d0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioForecastMember_98d4f41f-2d6a-40e7-a6c2-245ddf421ec8" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ScenarioForecastMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ScenarioUnspecifiedDomain_ee9a14a6-a53e-4f1b-8db2-311cd14d15d0" xlink:to="loc_srt_ScenarioForecastMember_98d4f41f-2d6a-40e7-a6c2-245ddf421ec8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryAssetAxis_e97fb96c-7f21-4846-882c-0675f7129504" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatoryAssetAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lnt_IncomeTaxTable_b322352b-ac26-4359-abaa-f2a53559d1c6" xlink:to="loc_us-gaap_RegulatoryAssetAxis_e97fb96c-7f21-4846-882c-0675f7129504" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryAssetDomain_e97fb96c-7f21-4846-882c-0675f7129504_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatoryAssetDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RegulatoryAssetAxis_e97fb96c-7f21-4846-882c-0675f7129504" xlink:to="loc_us-gaap_RegulatoryAssetDomain_e97fb96c-7f21-4846-882c-0675f7129504_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryAssetDomain_d2b06bdb-c381-4f41-978c-4c3e98fbdfbc" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatoryAssetDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RegulatoryAssetAxis_e97fb96c-7f21-4846-882c-0675f7129504" xlink:to="loc_us-gaap_RegulatoryAssetDomain_d2b06bdb-c381-4f41-978c-4c3e98fbdfbc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredIncomeTaxChargesMember_ce3d8449-5360-4eca-b579-d0dbd1247128" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DeferredIncomeTaxChargesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryAssetDomain_d2b06bdb-c381-4f41-978c-4c3e98fbdfbc" xlink:to="loc_us-gaap_DeferredIncomeTaxChargesMember_ce3d8449-5360-4eca-b579-d0dbd1247128" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/IncomeTaxesScheduleOfEffectiveIncomeTaxRatesDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#IncomeTaxesScheduleOfEffectiveIncomeTaxRatesDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/IncomeTaxesScheduleOfEffectiveIncomeTaxRatesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_lnt_EffectiveTaxRateLineItems_6de53ede-d13e-48b9-b6ae-167509b9033a" xlink:href="lnt-20240331.xsd#lnt_EffectiveTaxRateLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_EffectiveTaxRateTable_a794f238-e6ca-4a83-a6fc-f0ab591b5e9e" xlink:href="lnt-20240331.xsd#lnt_EffectiveTaxRateTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_lnt_EffectiveTaxRateLineItems_6de53ede-d13e-48b9-b6ae-167509b9033a" xlink:to="loc_lnt_EffectiveTaxRateTable_a794f238-e6ca-4a83-a6fc-f0ab591b5e9e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_2fd68a87-4c31-4fc0-9abb-b55ea8b9ab18" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lnt_EffectiveTaxRateTable_a794f238-e6ca-4a83-a6fc-f0ab591b5e9e" xlink:to="loc_dei_LegalEntityAxis_2fd68a87-4c31-4fc0-9abb-b55ea8b9ab18" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_2fd68a87-4c31-4fc0-9abb-b55ea8b9ab18_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_2fd68a87-4c31-4fc0-9abb-b55ea8b9ab18" xlink:to="loc_dei_EntityDomain_2fd68a87-4c31-4fc0-9abb-b55ea8b9ab18_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_e1db247b-609e-43f7-884c-61066a43b200" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_2fd68a87-4c31-4fc0-9abb-b55ea8b9ab18" xlink:to="loc_dei_EntityDomain_e1db247b-609e-43f7-884c-61066a43b200" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_61822dd9-1570-45d6-b25a-4ff3dbd73835" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_e1db247b-609e-43f7-884c-61066a43b200" xlink:to="loc_lnt_IplMember_61822dd9-1570-45d6-b25a-4ff3dbd73835" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_c18306dc-88b4-451a-b6a7-0c89919bd76b" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_e1db247b-609e-43f7-884c-61066a43b200" xlink:to="loc_lnt_WplMember_c18306dc-88b4-451a-b6a7-0c89919bd76b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EffectiveIncomeTaxRateContinuingOperations_2c76ce33-5fc3-47e7-a24f-a1409e8008dc" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EffectiveIncomeTaxRateContinuingOperations"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lnt_EffectiveTaxRateLineItems_6de53ede-d13e-48b9-b6ae-167509b9033a" xlink:to="loc_us-gaap_EffectiveIncomeTaxRateContinuingOperations_2c76ce33-5fc3-47e7-a24f-a1409e8008dc" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/IncomeTaxesSummaryOfTaxCreditCarryforwardsDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#IncomeTaxesSummaryOfTaxCreditCarryforwardsDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/IncomeTaxesSummaryOfTaxCreditCarryforwardsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLossCarryforwardsLineItems_9ade7d64-7e0e-493d-9c4e-e5bef4f318ab" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OperatingLossCarryforwardsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLossCarryforwardsTable_936446eb-923a-479d-b9c6-fd5d11827587" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OperatingLossCarryforwardsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_OperatingLossCarryforwardsLineItems_9ade7d64-7e0e-493d-9c4e-e5bef4f318ab" xlink:to="loc_us-gaap_OperatingLossCarryforwardsTable_936446eb-923a-479d-b9c6-fd5d11827587" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_69c19aa0-e344-4d5d-be04-cd81e8a0319b" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_OperatingLossCarryforwardsTable_936446eb-923a-479d-b9c6-fd5d11827587" xlink:to="loc_dei_LegalEntityAxis_69c19aa0-e344-4d5d-be04-cd81e8a0319b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_69c19aa0-e344-4d5d-be04-cd81e8a0319b_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_69c19aa0-e344-4d5d-be04-cd81e8a0319b" xlink:to="loc_dei_EntityDomain_69c19aa0-e344-4d5d-be04-cd81e8a0319b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_35290183-95a3-4340-9aaf-202a9d6208f6" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_69c19aa0-e344-4d5d-be04-cd81e8a0319b" xlink:to="loc_dei_EntityDomain_35290183-95a3-4340-9aaf-202a9d6208f6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_fa88b576-3b55-4400-bec5-adf592aa6e5a" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_35290183-95a3-4340-9aaf-202a9d6208f6" xlink:to="loc_lnt_IplMember_fa88b576-3b55-4400-bec5-adf592aa6e5a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_40f6e906-100f-46db-af7c-c4835abe892e" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_35290183-95a3-4340-9aaf-202a9d6208f6" xlink:to="loc_lnt_WplMember_40f6e906-100f-46db-af7c-c4835abe892e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityAxis_c40a259d-4fc7-4699-a5eb-35e948e73be8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeTaxAuthorityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_OperatingLossCarryforwardsTable_936446eb-923a-479d-b9c6-fd5d11827587" xlink:to="loc_us-gaap_IncomeTaxAuthorityAxis_c40a259d-4fc7-4699-a5eb-35e948e73be8" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityDomain_c40a259d-4fc7-4699-a5eb-35e948e73be8_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeTaxAuthorityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeTaxAuthorityAxis_c40a259d-4fc7-4699-a5eb-35e948e73be8" xlink:to="loc_us-gaap_IncomeTaxAuthorityDomain_c40a259d-4fc7-4699-a5eb-35e948e73be8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityDomain_6669b348-b412-4688-b5ca-78974f313274" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeTaxAuthorityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeTaxAuthorityAxis_c40a259d-4fc7-4699-a5eb-35e948e73be8" xlink:to="loc_us-gaap_IncomeTaxAuthorityDomain_6669b348-b412-4688-b5ca-78974f313274" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StateAndLocalJurisdictionMember_f55031b6-77a2-4a82-b9f0-e162eca29d66" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StateAndLocalJurisdictionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeTaxAuthorityDomain_6669b348-b412-4688-b5ca-78974f313274" xlink:to="loc_us-gaap_StateAndLocalJurisdictionMember_f55031b6-77a2-4a82-b9f0-e162eca29d66" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DomesticCountryMember_7c2f0ed0-d1b4-4783-81e1-65ecd56c8e5a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DomesticCountryMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeTaxAuthorityDomain_6669b348-b412-4688-b5ca-78974f313274" xlink:to="loc_us-gaap_DomesticCountryMember_7c2f0ed0-d1b4-4783-81e1-65ecd56c8e5a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLossCarryforwards_6699a55c-599b-4e28-961f-681a5f79f9cf" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OperatingLossCarryforwards"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingLossCarryforwardsLineItems_9ade7d64-7e0e-493d-9c4e-e5bef4f318ab" xlink:to="loc_us-gaap_OperatingLossCarryforwards_6699a55c-599b-4e28-961f-681a5f79f9cf" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TaxCreditCarryforwardAmount_5acd1938-ce5f-4a9e-a6f0-caca7fbda62f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TaxCreditCarryforwardAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingLossCarryforwardsLineItems_9ade7d64-7e0e-493d-9c4e-e5bef4f318ab" xlink:to="loc_us-gaap_TaxCreditCarryforwardAmount_5acd1938-ce5f-4a9e-a6f0-caca7fbda62f" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/BenefitPlansNarrativeDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#BenefitPlansNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/BenefitPlansNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_e36bdd5b-a00a-463a-8ee1-66809c17a965" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DefinedBenefitPlanDisclosureLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_3d8ea004-e5bd-4ff4-9b77-2f33c45f3eb8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_e36bdd5b-a00a-463a-8ee1-66809c17a965" xlink:to="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_3d8ea004-e5bd-4ff4-9b77-2f33c45f3eb8" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardDateAxis_94ad0343-38ff-4448-944e-3fe69787d52c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AwardDateAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_3d8ea004-e5bd-4ff4-9b77-2f33c45f3eb8" xlink:to="loc_us-gaap_AwardDateAxis_94ad0343-38ff-4448-944e-3fe69787d52c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardDateDomain_94ad0343-38ff-4448-944e-3fe69787d52c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AwardDateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardDateAxis_94ad0343-38ff-4448-944e-3fe69787d52c" xlink:to="loc_us-gaap_AwardDateDomain_94ad0343-38ff-4448-944e-3fe69787d52c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardDateDomain_eed8d3c6-b99a-4b28-996d-88ea5cbd184b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AwardDateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardDateAxis_94ad0343-38ff-4448-944e-3fe69787d52c" xlink:to="loc_us-gaap_AwardDateDomain_eed8d3c6-b99a-4b28-996d-88ea5cbd184b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_90db3106-ed86-4491-a322-9566a41b46bc" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_3d8ea004-e5bd-4ff4-9b77-2f33c45f3eb8" xlink:to="loc_us-gaap_AwardTypeAxis_90db3106-ed86-4491-a322-9566a41b46bc" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_90db3106-ed86-4491-a322-9566a41b46bc_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_90db3106-ed86-4491-a322-9566a41b46bc" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_90db3106-ed86-4491-a322-9566a41b46bc_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_9bc2e27a-1433-48a2-af3a-274b89445709" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_90db3106-ed86-4491-a322-9566a41b46bc" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_9bc2e27a-1433-48a2-af3a-274b89445709" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_OmnibusIncentivePlanMember_7aa3184d-af52-4b51-862c-2e0fde300958" xlink:href="lnt-20240331.xsd#lnt_OmnibusIncentivePlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_9bc2e27a-1433-48a2-af3a-274b89445709" xlink:to="loc_lnt_OmnibusIncentivePlanMember_7aa3184d-af52-4b51-862c-2e0fde300958" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_17bfe993-f085-4a55-b31b-f835838d25d9" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_3d8ea004-e5bd-4ff4-9b77-2f33c45f3eb8" xlink:to="loc_dei_LegalEntityAxis_17bfe993-f085-4a55-b31b-f835838d25d9" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_17bfe993-f085-4a55-b31b-f835838d25d9_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_17bfe993-f085-4a55-b31b-f835838d25d9" xlink:to="loc_dei_EntityDomain_17bfe993-f085-4a55-b31b-f835838d25d9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_f4eaaeab-39cb-46ce-a9e2-aa390a8b71b7" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_17bfe993-f085-4a55-b31b-f835838d25d9" xlink:to="loc_dei_EntityDomain_f4eaaeab-39cb-46ce-a9e2-aa390a8b71b7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_2dd0a49a-923c-4b9a-b593-04c85ccde7ea" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_f4eaaeab-39cb-46ce-a9e2-aa390a8b71b7" xlink:to="loc_lnt_IplMember_2dd0a49a-923c-4b9a-b593-04c85ccde7ea" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_82a67973-9451-47ff-9830-d72f78c42278" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_f4eaaeab-39cb-46ce-a9e2-aa390a8b71b7" xlink:to="loc_lnt_WplMember_82a67973-9451-47ff-9830-d72f78c42278" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_734ed40c-c342-44a1-8367-4b4ac603ba38" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_3d8ea004-e5bd-4ff4-9b77-2f33c45f3eb8" xlink:to="loc_srt_RangeAxis_734ed40c-c342-44a1-8367-4b4ac603ba38" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_734ed40c-c342-44a1-8367-4b4ac603ba38_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_734ed40c-c342-44a1-8367-4b4ac603ba38" xlink:to="loc_srt_RangeMember_734ed40c-c342-44a1-8367-4b4ac603ba38_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_d311cd07-03c3-4287-b62a-04a179895980" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_734ed40c-c342-44a1-8367-4b4ac603ba38" xlink:to="loc_srt_RangeMember_d311cd07-03c3-4287-b62a-04a179895980" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_e2cc08a8-7bd3-4d6f-86ee-abbc062dfa8e" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_d311cd07-03c3-4287-b62a-04a179895980" xlink:to="loc_srt_MinimumMember_e2cc08a8-7bd3-4d6f-86ee-abbc062dfa8e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_7501a23e-3956-4e52-9f26-adf83445de2c" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_d311cd07-03c3-4287-b62a-04a179895980" xlink:to="loc_srt_MaximumMember_7501a23e-3956-4e52-9f26-adf83445de2c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_647f75ce-657f-4a64-9f47-dfa2f1ec7656" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_e36bdd5b-a00a-463a-8ee1-66809c17a965" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_647f75ce-657f-4a64-9f47-dfa2f1ec7656" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_4a10d0f0-f843-48ad-b88e-585f8df6d659" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_e36bdd5b-a00a-463a-8ee1-66809c17a965" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_4a10d0f0-f843-48ad-b88e-585f8df6d659" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberDilutedSharesOutstandingAdjustment_0d233dbe-374a-45c6-97f5-62f141d079b6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_WeightedAverageNumberDilutedSharesOutstandingAdjustment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_e36bdd5b-a00a-463a-8ee1-66809c17a965" xlink:to="loc_us-gaap_WeightedAverageNumberDilutedSharesOutstandingAdjustment_0d233dbe-374a-45c6-97f5-62f141d079b6" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/BenefitPlansDefinedBenefitPensionAndOtherPostretirementBenefitsPlansDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#BenefitPlansDefinedBenefitPensionAndOtherPostretirementBenefitsPlansDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/BenefitPlansDefinedBenefitPensionAndOtherPostretirementBenefitsPlansDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_9f0b6dea-0919-4d96-9c68-120efd320fa3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DefinedBenefitPlanDisclosureLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_14e58f48-2528-439a-9c88-efaa7aaec305" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_9f0b6dea-0919-4d96-9c68-120efd320fa3" xlink:to="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_14e58f48-2528-439a-9c88-efaa7aaec305" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_ed9808e7-f35a-4b27-9348-b815d1e6adaf" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_14e58f48-2528-439a-9c88-efaa7aaec305" xlink:to="loc_dei_LegalEntityAxis_ed9808e7-f35a-4b27-9348-b815d1e6adaf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_ed9808e7-f35a-4b27-9348-b815d1e6adaf_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_ed9808e7-f35a-4b27-9348-b815d1e6adaf" xlink:to="loc_dei_EntityDomain_ed9808e7-f35a-4b27-9348-b815d1e6adaf_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_89c2eba0-eb8b-47af-96e7-d5c661bfc057" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_ed9808e7-f35a-4b27-9348-b815d1e6adaf" xlink:to="loc_dei_EntityDomain_89c2eba0-eb8b-47af-96e7-d5c661bfc057" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_fe154d03-2a19-42cd-8f58-6e28e1fb9ed2" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_89c2eba0-eb8b-47af-96e7-d5c661bfc057" xlink:to="loc_lnt_IplMember_fe154d03-2a19-42cd-8f58-6e28e1fb9ed2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_8a192f07-a4e7-4491-b007-a386387ded0d" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_89c2eba0-eb8b-47af-96e7-d5c661bfc057" xlink:to="loc_lnt_WplMember_8a192f07-a4e7-4491-b007-a386387ded0d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeAxis_b971a789-8742-4434-a662-73a50633ca65" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RetirementPlanTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_14e58f48-2528-439a-9c88-efaa7aaec305" xlink:to="loc_us-gaap_RetirementPlanTypeAxis_b971a789-8742-4434-a662-73a50633ca65" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeDomain_b971a789-8742-4434-a662-73a50633ca65_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RetirementPlanTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RetirementPlanTypeAxis_b971a789-8742-4434-a662-73a50633ca65" xlink:to="loc_us-gaap_RetirementPlanTypeDomain_b971a789-8742-4434-a662-73a50633ca65_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeDomain_bdf58ae0-ca12-439f-9f2c-e2a6442dae7c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RetirementPlanTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RetirementPlanTypeAxis_b971a789-8742-4434-a662-73a50633ca65" xlink:to="loc_us-gaap_RetirementPlanTypeDomain_bdf58ae0-ca12-439f-9f2c-e2a6442dae7c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PensionPlansDefinedBenefitMember_df24f2d6-8449-42fa-9397-64e4677e8ece" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PensionPlansDefinedBenefitMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanTypeDomain_bdf58ae0-ca12-439f-9f2c-e2a6442dae7c" xlink:to="loc_us-gaap_PensionPlansDefinedBenefitMember_df24f2d6-8449-42fa-9397-64e4677e8ece" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherPostretirementBenefitPlansDefinedBenefitMember_091b37a7-9ee6-46a1-906b-d8f7f961efb7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherPostretirementBenefitPlansDefinedBenefitMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanTypeDomain_bdf58ae0-ca12-439f-9f2c-e2a6442dae7c" xlink:to="loc_us-gaap_OtherPostretirementBenefitPlansDefinedBenefitMember_091b37a7-9ee6-46a1-906b-d8f7f961efb7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanServiceCost_33df752d-a890-4714-8150-b42722018cfd" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DefinedBenefitPlanServiceCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_9f0b6dea-0919-4d96-9c68-120efd320fa3" xlink:to="loc_us-gaap_DefinedBenefitPlanServiceCost_33df752d-a890-4714-8150-b42722018cfd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanInterestCost_661b7561-4b4a-4f54-bc74-84b934b52af2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DefinedBenefitPlanInterestCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_9f0b6dea-0919-4d96-9c68-120efd320fa3" xlink:to="loc_us-gaap_DefinedBenefitPlanInterestCost_661b7561-4b4a-4f54-bc74-84b934b52af2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanExpectedReturnOnPlanAssets_4fd2c228-615a-4241-8a4d-d12670894213" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DefinedBenefitPlanExpectedReturnOnPlanAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_9f0b6dea-0919-4d96-9c68-120efd320fa3" xlink:to="loc_us-gaap_DefinedBenefitPlanExpectedReturnOnPlanAssets_4fd2c228-615a-4241-8a4d-d12670894213" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanAmortizationOfGainsLosses_55df9c18-35e2-408e-8ad8-c2f74f373269" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DefinedBenefitPlanAmortizationOfGainsLosses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_9f0b6dea-0919-4d96-9c68-120efd320fa3" xlink:to="loc_us-gaap_DefinedBenefitPlanAmortizationOfGainsLosses_55df9c18-35e2-408e-8ad8-c2f74f373269" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCost_467156d3-86de-4c6d-8158-e7fb2d1858b0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DefinedBenefitPlanNetPeriodicBenefitCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_9f0b6dea-0919-4d96-9c68-120efd320fa3" xlink:to="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCost_467156d3-86de-4c6d-8158-e7fb2d1858b0" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/BenefitPlansDefinedContributionRetirementPlansDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#BenefitPlansDefinedContributionRetirementPlansDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/BenefitPlansDefinedContributionRetirementPlansDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_67da5045-4d7d-48b3-a125-de631d52d028" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_9a18be3e-17a6-4b7c-afda-22f1be384d66" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_67da5045-4d7d-48b3-a125-de631d52d028" xlink:to="loc_us-gaap_StatementTable_9a18be3e-17a6-4b7c-afda-22f1be384d66" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_af4e7d60-ebfc-426f-903e-11daea960e9c" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_9a18be3e-17a6-4b7c-afda-22f1be384d66" xlink:to="loc_dei_LegalEntityAxis_af4e7d60-ebfc-426f-903e-11daea960e9c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_af4e7d60-ebfc-426f-903e-11daea960e9c_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_af4e7d60-ebfc-426f-903e-11daea960e9c" xlink:to="loc_dei_EntityDomain_af4e7d60-ebfc-426f-903e-11daea960e9c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_5180f0f3-cbac-405b-b527-0bc2b0e1137d" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_af4e7d60-ebfc-426f-903e-11daea960e9c" xlink:to="loc_dei_EntityDomain_5180f0f3-cbac-405b-b527-0bc2b0e1137d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_87a63d5f-bc32-4ad5-b8aa-13de1180bdb7" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_5180f0f3-cbac-405b-b527-0bc2b0e1137d" xlink:to="loc_lnt_IplMember_87a63d5f-bc32-4ad5-b8aa-13de1180bdb7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_2ab3f66c-e196-49cd-a1d0-badd75ce377e" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_5180f0f3-cbac-405b-b527-0bc2b0e1137d" xlink:to="loc_lnt_WplMember_2ab3f66c-e196-49cd-a1d0-badd75ce377e" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/BenefitPlansRecognizedCompensationExpenseAndIncomeTaxBenefitsDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#BenefitPlansRecognizedCompensationExpenseAndIncomeTaxBenefitsDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/BenefitPlansRecognizedCompensationExpenseAndIncomeTaxBenefitsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_806368d0-0f39-4f53-8875-0e27b04d51a8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_625c779a-e03d-4393-8017-ee5c486346d4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_806368d0-0f39-4f53-8875-0e27b04d51a8" xlink:to="loc_us-gaap_StatementTable_625c779a-e03d-4393-8017-ee5c486346d4" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_63c4735a-b78d-40d2-9e61-644064aa44ea" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_625c779a-e03d-4393-8017-ee5c486346d4" xlink:to="loc_dei_LegalEntityAxis_63c4735a-b78d-40d2-9e61-644064aa44ea" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_63c4735a-b78d-40d2-9e61-644064aa44ea_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_63c4735a-b78d-40d2-9e61-644064aa44ea" xlink:to="loc_dei_EntityDomain_63c4735a-b78d-40d2-9e61-644064aa44ea_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_5e1aa908-ea82-4c61-8305-290f39c96632" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_63c4735a-b78d-40d2-9e61-644064aa44ea" xlink:to="loc_dei_EntityDomain_5e1aa908-ea82-4c61-8305-290f39c96632" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_32d9725d-7455-43a1-95ef-294edf603eda" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_5e1aa908-ea82-4c61-8305-290f39c96632" xlink:to="loc_lnt_IplMember_32d9725d-7455-43a1-95ef-294edf603eda" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_ba5847a2-a06a-471c-8cb1-ecd605148fd3" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_5e1aa908-ea82-4c61-8305-290f39c96632" xlink:to="loc_lnt_WplMember_ba5847a2-a06a-471c-8cb1-ecd605148fd3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllocatedShareBasedCompensationExpense_b63747f4-c23f-4bd1-bbd2-08a740549134" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AllocatedShareBasedCompensationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_806368d0-0f39-4f53-8875-0e27b04d51a8" xlink:to="loc_us-gaap_AllocatedShareBasedCompensationExpense_b63747f4-c23f-4bd1-bbd2-08a740549134" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationTaxBenefitFromCompensationExpense_e2ae0711-f3f5-49c0-a51b-8eaeca322eac" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EmployeeServiceShareBasedCompensationTaxBenefitFromCompensationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_806368d0-0f39-4f53-8875-0e27b04d51a8" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationTaxBenefitFromCompensationExpense_e2ae0711-f3f5-49c0-a51b-8eaeca322eac" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/BenefitPlansSummaryOfEquitybasedCompensationPlansGrantsDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#BenefitPlansSummaryOfEquitybasedCompensationPlansGrantsDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/BenefitPlansSummaryOfEquitybasedCompensationPlansGrantsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a46978c1-eeb8-4b10-8e62-dde2e16c415d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_39b14b51-12a7-4793-b3da-9a0f540ebe64" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a46978c1-eeb8-4b10-8e62-dde2e16c415d" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_39b14b51-12a7-4793-b3da-9a0f540ebe64" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardDateAxis_222d2f57-a615-457e-b15b-5c045c967166" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AwardDateAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_39b14b51-12a7-4793-b3da-9a0f540ebe64" xlink:to="loc_us-gaap_AwardDateAxis_222d2f57-a615-457e-b15b-5c045c967166" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardDateDomain_222d2f57-a615-457e-b15b-5c045c967166_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AwardDateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardDateAxis_222d2f57-a615-457e-b15b-5c045c967166" xlink:to="loc_us-gaap_AwardDateDomain_222d2f57-a615-457e-b15b-5c045c967166_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardDateDomain_40c1ec2e-eb7b-4872-a0fc-6309a599ea1f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AwardDateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardDateAxis_222d2f57-a615-457e-b15b-5c045c967166" xlink:to="loc_us-gaap_AwardDateDomain_40c1ec2e-eb7b-4872-a0fc-6309a599ea1f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_6687ed3c-14b2-4f02-a852-b113957f7c5a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_39b14b51-12a7-4793-b3da-9a0f540ebe64" xlink:to="loc_us-gaap_AwardTypeAxis_6687ed3c-14b2-4f02-a852-b113957f7c5a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_6687ed3c-14b2-4f02-a852-b113957f7c5a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_6687ed3c-14b2-4f02-a852-b113957f7c5a" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_6687ed3c-14b2-4f02-a852-b113957f7c5a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_f30e4c9a-8afd-48e3-abf4-e50d5b33a88c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_6687ed3c-14b2-4f02-a852-b113957f7c5a" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_f30e4c9a-8afd-48e3-abf4-e50d5b33a88c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PerformanceSharesMember_8a2ea0fb-1a5e-42ab-90e6-206796bbb1d9" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PerformanceSharesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_f30e4c9a-8afd-48e3-abf4-e50d5b33a88c" xlink:to="loc_us-gaap_PerformanceSharesMember_8a2ea0fb-1a5e-42ab-90e6-206796bbb1d9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_PerformanceRestrictedStockUnitMember_2ea6ec76-c88b-4720-ba4b-219850d4a635" xlink:href="lnt-20240331.xsd#lnt_PerformanceRestrictedStockUnitMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_f30e4c9a-8afd-48e3-abf4-e50d5b33a88c" xlink:to="loc_lnt_PerformanceRestrictedStockUnitMember_2ea6ec76-c88b-4720-ba4b-219850d4a635" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_32e17386-0835-4760-968d-5d8e529f58c6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_f30e4c9a-8afd-48e3-abf4-e50d5b33a88c" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_32e17386-0835-4760-968d-5d8e529f58c6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod_42951000-349e-4d23-b2ee-0d5780a4deea" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a46978c1-eeb8-4b10-8e62-dde2e16c415d" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod_42951000-349e-4d23-b2ee-0d5780a4deea" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue_c0983cad-9e03-4352-9f58-087c1044d77d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_a46978c1-eeb8-4b10-8e62-dde2e16c415d" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue_c0983cad-9e03-4352-9f58-087c1044d77d" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/DerivativeInstrumentsNarrativeDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#DerivativeInstrumentsNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/DerivativeInstrumentsNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeLineItems_ac955931-8d3b-4cd5-8d1a-3a5e9bc8995f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeTable_310cf141-8f27-43af-9913-da9dfe348c66" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DerivativeLineItems_ac955931-8d3b-4cd5-8d1a-3a5e9bc8995f" xlink:to="loc_us-gaap_DerivativeTable_310cf141-8f27-43af-9913-da9dfe348c66" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_908a5a5c-9420-4349-aaf5-43113c8e5247" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeTable_310cf141-8f27-43af-9913-da9dfe348c66" xlink:to="loc_dei_LegalEntityAxis_908a5a5c-9420-4349-aaf5-43113c8e5247" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_908a5a5c-9420-4349-aaf5-43113c8e5247_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_908a5a5c-9420-4349-aaf5-43113c8e5247" xlink:to="loc_dei_EntityDomain_908a5a5c-9420-4349-aaf5-43113c8e5247_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_9eb84d6c-d83f-4081-bf53-64dfb4d83ec7" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_908a5a5c-9420-4349-aaf5-43113c8e5247" xlink:to="loc_dei_EntityDomain_9eb84d6c-d83f-4081-bf53-64dfb4d83ec7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_AlliantEnergyFinanceLLCMember_bed493b4-b16e-4a33-b193-050623a59c80" xlink:href="lnt-20240331.xsd#lnt_AlliantEnergyFinanceLLCMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_9eb84d6c-d83f-4081-bf53-64dfb4d83ec7" xlink:to="loc_lnt_AlliantEnergyFinanceLLCMember_bed493b4-b16e-4a33-b193-050623a59c80" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeInstrumentRiskAxis_8799039d-7c48-4f86-be9a-395555daea70" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeInstrumentRiskAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeTable_310cf141-8f27-43af-9913-da9dfe348c66" xlink:to="loc_us-gaap_DerivativeInstrumentRiskAxis_8799039d-7c48-4f86-be9a-395555daea70" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_8799039d-7c48-4f86-be9a-395555daea70_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_8799039d-7c48-4f86-be9a-395555daea70" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_8799039d-7c48-4f86-be9a-395555daea70_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_b88b0517-50e5-4064-b438-be7d058613a4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_8799039d-7c48-4f86-be9a-395555daea70" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_b88b0517-50e5-4064-b438-be7d058613a4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestRateSwapMember_a7f3de00-5324-49a6-b80c-df3a5c6d8397" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_InterestRateSwapMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeContractTypeDomain_b88b0517-50e5-4064-b438-be7d058613a4" xlink:to="loc_us-gaap_InterestRateSwapMember_a7f3de00-5324-49a6-b80c-df3a5c6d8397" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_dc624326-e81d-4162-af28-eb29ac20ef6f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeTable_310cf141-8f27-43af-9913-da9dfe348c66" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_dc624326-e81d-4162-af28-eb29ac20ef6f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_dc624326-e81d-4162-af28-eb29ac20ef6f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_dc624326-e81d-4162-af28-eb29ac20ef6f" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_dc624326-e81d-4162-af28-eb29ac20ef6f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_3c070649-fbd3-44a4-8af7-cde4ffb57771" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_dc624326-e81d-4162-af28-eb29ac20ef6f" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_3c070649-fbd3-44a4-8af7-cde4ffb57771" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_TermLoanCreditAgreementMember_9b82e68c-76a3-4fc8-90c4-94184d61a423" xlink:href="lnt-20240331.xsd#lnt_TermLoanCreditAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_3c070649-fbd3-44a4-8af7-cde4ffb57771" xlink:to="loc_lnt_TermLoanCreditAgreementMember_9b82e68c-76a3-4fc8-90c4-94184d61a423" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredCosts_ed5001de-030f-42f2-a3e9-4e6d099f9957" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DeferredCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_ac955931-8d3b-4cd5-8d1a-3a5e9bc8995f" xlink:to="loc_us-gaap_DeferredCosts_ed5001de-030f-42f2-a3e9-4e6d099f9957" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_671e0f74-8a5f-425b-b882-40e1a86e96a1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_ac955931-8d3b-4cd5-8d1a-3a5e9bc8995f" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_671e0f74-8a5f-425b-b882-40e1a86e96a1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpense_e68bff2d-0c26-47fd-807e-0dc770447d60" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_InterestExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_ac955931-8d3b-4cd5-8d1a-3a5e9bc8995f" xlink:to="loc_us-gaap_InterestExpense_e68bff2d-0c26-47fd-807e-0dc770447d60" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/DerivativeInstrumentsNotionalAmountsOfDerivativeInstrumentsDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#DerivativeInstrumentsNotionalAmountsOfDerivativeInstrumentsDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/DerivativeInstrumentsNotionalAmountsOfDerivativeInstrumentsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_lnt_NotionalAmountOfDerivativesLineItems_895acaf0-6147-409e-83d8-87260383aa1b" xlink:href="lnt-20240331.xsd#lnt_NotionalAmountOfDerivativesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_ScheduleOfNotionalAmountOfDerivativesTable_a1dde5c9-fc02-477f-85b5-d58944f14de0" xlink:href="lnt-20240331.xsd#lnt_ScheduleOfNotionalAmountOfDerivativesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_lnt_NotionalAmountOfDerivativesLineItems_895acaf0-6147-409e-83d8-87260383aa1b" xlink:to="loc_lnt_ScheduleOfNotionalAmountOfDerivativesTable_a1dde5c9-fc02-477f-85b5-d58944f14de0" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeInstrumentRiskAxis_8c728053-732c-43a1-921d-75fe651ea614" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeInstrumentRiskAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lnt_ScheduleOfNotionalAmountOfDerivativesTable_a1dde5c9-fc02-477f-85b5-d58944f14de0" xlink:to="loc_us-gaap_DerivativeInstrumentRiskAxis_8c728053-732c-43a1-921d-75fe651ea614" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_8c728053-732c-43a1-921d-75fe651ea614_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_8c728053-732c-43a1-921d-75fe651ea614" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_8c728053-732c-43a1-921d-75fe651ea614_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_10ad6076-f836-44d1-9800-166322f102aa" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_8c728053-732c-43a1-921d-75fe651ea614" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_10ad6076-f836-44d1-9800-166322f102aa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommodityContractMember_2cb3e196-98ec-424d-a515-90166b3d3cce" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommodityContractMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeContractTypeDomain_10ad6076-f836-44d1-9800-166322f102aa" xlink:to="loc_us-gaap_CommodityContractMember_2cb3e196-98ec-424d-a515-90166b3d3cce" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestRateSwapMember_d7c6f965-a318-40aa-af50-7c082f2ea5e6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_InterestRateSwapMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeContractTypeDomain_10ad6076-f836-44d1-9800-166322f102aa" xlink:to="loc_us-gaap_InterestRateSwapMember_d7c6f965-a318-40aa-af50-7c082f2ea5e6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_EnergyAxis_edf8c28e-41ba-4097-8882-be0e6ba8f6f9" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_EnergyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lnt_ScheduleOfNotionalAmountOfDerivativesTable_a1dde5c9-fc02-477f-85b5-d58944f14de0" xlink:to="loc_srt_EnergyAxis_edf8c28e-41ba-4097-8882-be0e6ba8f6f9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_EnergyDomain_edf8c28e-41ba-4097-8882-be0e6ba8f6f9_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_EnergyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_EnergyAxis_edf8c28e-41ba-4097-8882-be0e6ba8f6f9" xlink:to="loc_srt_EnergyDomain_edf8c28e-41ba-4097-8882-be0e6ba8f6f9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_EnergyDomain_1e1fc4f5-d489-4bf2-b77b-37a2d6d1465b" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_EnergyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_EnergyAxis_edf8c28e-41ba-4097-8882-be0e6ba8f6f9" xlink:to="loc_srt_EnergyDomain_1e1fc4f5-d489-4bf2-b77b-37a2d6d1465b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ElectricityMember_ba052834-5dd4-4dcb-a829-cc1098588bde" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ElectricityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_EnergyDomain_1e1fc4f5-d489-4bf2-b77b-37a2d6d1465b" xlink:to="loc_us-gaap_ElectricityMember_ba052834-5dd4-4dcb-a829-cc1098588bde" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_FtrsMwhsMember_917929af-e184-4b34-8da3-8b80b8dd02d6" xlink:href="lnt-20240331.xsd#lnt_FtrsMwhsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_EnergyDomain_1e1fc4f5-d489-4bf2-b77b-37a2d6d1465b" xlink:to="loc_lnt_FtrsMwhsMember_917929af-e184-4b34-8da3-8b80b8dd02d6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_NaturalGasPerThousandCubicFeetMember_ddeb2d92-fac4-4479-88c3-9edec4534110" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_NaturalGasPerThousandCubicFeetMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_EnergyDomain_1e1fc4f5-d489-4bf2-b77b-37a2d6d1465b" xlink:to="loc_srt_NaturalGasPerThousandCubicFeetMember_ddeb2d92-fac4-4479-88c3-9edec4534110" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TradingActivityByTypeAxis_0e17147d-814b-477d-a1f4-4a5d2d1af46e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TradingActivityByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lnt_ScheduleOfNotionalAmountOfDerivativesTable_a1dde5c9-fc02-477f-85b5-d58944f14de0" xlink:to="loc_us-gaap_TradingActivityByTypeAxis_0e17147d-814b-477d-a1f4-4a5d2d1af46e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TradingActivityByTypeDomain_0e17147d-814b-477d-a1f4-4a5d2d1af46e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TradingActivityByTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_TradingActivityByTypeAxis_0e17147d-814b-477d-a1f4-4a5d2d1af46e" xlink:to="loc_us-gaap_TradingActivityByTypeDomain_0e17147d-814b-477d-a1f4-4a5d2d1af46e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TradingActivityByTypeDomain_f0650180-272d-406b-a52d-1383d0bbd60d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TradingActivityByTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_TradingActivityByTypeAxis_0e17147d-814b-477d-a1f4-4a5d2d1af46e" xlink:to="loc_us-gaap_TradingActivityByTypeDomain_f0650180-272d-406b-a52d-1383d0bbd60d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommodityMember_fcfae430-64c6-462f-b176-acef41c70e7f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommodityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TradingActivityByTypeDomain_f0650180-272d-406b-a52d-1383d0bbd60d" xlink:to="loc_us-gaap_CommodityMember_fcfae430-64c6-462f-b176-acef41c70e7f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_713f0df0-67ba-4e31-8378-edfbcdc11359" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lnt_ScheduleOfNotionalAmountOfDerivativesTable_a1dde5c9-fc02-477f-85b5-d58944f14de0" xlink:to="loc_dei_LegalEntityAxis_713f0df0-67ba-4e31-8378-edfbcdc11359" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_713f0df0-67ba-4e31-8378-edfbcdc11359_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_713f0df0-67ba-4e31-8378-edfbcdc11359" xlink:to="loc_dei_EntityDomain_713f0df0-67ba-4e31-8378-edfbcdc11359_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_253b4715-cbc8-4904-a965-f82db11e8184" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_713f0df0-67ba-4e31-8378-edfbcdc11359" xlink:to="loc_dei_EntityDomain_253b4715-cbc8-4904-a965-f82db11e8184" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_f68c8135-f213-4318-8741-3dbb76999f6b" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_253b4715-cbc8-4904-a965-f82db11e8184" xlink:to="loc_lnt_IplMember_f68c8135-f213-4318-8741-3dbb76999f6b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_5dc9b907-58cd-4a59-b6d1-3e8487b6c046" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_253b4715-cbc8-4904-a965-f82db11e8184" xlink:to="loc_lnt_WplMember_5dc9b907-58cd-4a59-b6d1-3e8487b6c046" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeNonmonetaryNotionalAmountEnergyMeasure_6fa430f2-0a7a-4bc9-8453-fa5c15229424" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeNonmonetaryNotionalAmountEnergyMeasure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lnt_NotionalAmountOfDerivativesLineItems_895acaf0-6147-409e-83d8-87260383aa1b" xlink:to="loc_us-gaap_DerivativeNonmonetaryNotionalAmountEnergyMeasure_6fa430f2-0a7a-4bc9-8453-fa5c15229424" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeNonmonetaryNotionalAmount_d6309c7d-b782-4c99-ba21-c4c358ce7746" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeNonmonetaryNotionalAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lnt_NotionalAmountOfDerivativesLineItems_895acaf0-6147-409e-83d8-87260383aa1b" xlink:to="loc_us-gaap_DerivativeNonmonetaryNotionalAmount_d6309c7d-b782-4c99-ba21-c4c358ce7746" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/DerivativeInstrumentsFairValueOfFinancialInstrumentsDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#DerivativeInstrumentsFairValueOfFinancialInstrumentsDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/DerivativeInstrumentsFairValueOfFinancialInstrumentsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativesFairValueLineItems_da3eec30-ac33-48d2-b86a-601361095b51" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativesFairValueLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValuesDerivativesBalanceSheetLocationByDerivativeContractTypeByHedgingDesignationTable_2a2600e4-ade1-4b87-9345-8ef13079e403" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValuesDerivativesBalanceSheetLocationByDerivativeContractTypeByHedgingDesignationTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DerivativesFairValueLineItems_da3eec30-ac33-48d2-b86a-601361095b51" xlink:to="loc_us-gaap_FairValuesDerivativesBalanceSheetLocationByDerivativeContractTypeByHedgingDesignationTable_2a2600e4-ade1-4b87-9345-8ef13079e403" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeInstrumentRiskAxis_a932b6b8-30a3-4425-8518-03ba3c9972c1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeInstrumentRiskAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValuesDerivativesBalanceSheetLocationByDerivativeContractTypeByHedgingDesignationTable_2a2600e4-ade1-4b87-9345-8ef13079e403" xlink:to="loc_us-gaap_DerivativeInstrumentRiskAxis_a932b6b8-30a3-4425-8518-03ba3c9972c1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_a932b6b8-30a3-4425-8518-03ba3c9972c1_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_a932b6b8-30a3-4425-8518-03ba3c9972c1" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_a932b6b8-30a3-4425-8518-03ba3c9972c1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_4f56598f-fe48-4d57-bddc-6ed7f7a2737c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_a932b6b8-30a3-4425-8518-03ba3c9972c1" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_4f56598f-fe48-4d57-bddc-6ed7f7a2737c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommodityContractMember_536ce072-8f3e-4c5d-820d-a56651fffc5a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommodityContractMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeContractTypeDomain_4f56598f-fe48-4d57-bddc-6ed7f7a2737c" xlink:to="loc_us-gaap_CommodityContractMember_536ce072-8f3e-4c5d-820d-a56651fffc5a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_27a9c429-2c22-4669-a8a8-619c22dcf28e" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValuesDerivativesBalanceSheetLocationByDerivativeContractTypeByHedgingDesignationTable_2a2600e4-ade1-4b87-9345-8ef13079e403" xlink:to="loc_dei_LegalEntityAxis_27a9c429-2c22-4669-a8a8-619c22dcf28e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_27a9c429-2c22-4669-a8a8-619c22dcf28e_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_27a9c429-2c22-4669-a8a8-619c22dcf28e" xlink:to="loc_dei_EntityDomain_27a9c429-2c22-4669-a8a8-619c22dcf28e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_41704c4b-d293-4e6e-b8fb-721a15ee317f" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_27a9c429-2c22-4669-a8a8-619c22dcf28e" xlink:to="loc_dei_EntityDomain_41704c4b-d293-4e6e-b8fb-721a15ee317f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_5737eda8-977d-4210-98f9-e49ffe8156ff" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_41704c4b-d293-4e6e-b8fb-721a15ee317f" xlink:to="loc_lnt_IplMember_5737eda8-977d-4210-98f9-e49ffe8156ff" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_6238832e-33c7-44ab-9953-c9197f03a805" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_41704c4b-d293-4e6e-b8fb-721a15ee317f" xlink:to="loc_lnt_WplMember_6238832e-33c7-44ab-9953-c9197f03a805" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeAssetsCurrent_6cf2a669-f623-4357-addd-274d0199d4b4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeAssetsCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativesFairValueLineItems_da3eec30-ac33-48d2-b86a-601361095b51" xlink:to="loc_us-gaap_DerivativeAssetsCurrent_6cf2a669-f623-4357-addd-274d0199d4b4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeAssetsNoncurrent_d8222923-b0de-4afd-8c3b-001d9dc4fec4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeAssetsNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativesFairValueLineItems_da3eec30-ac33-48d2-b86a-601361095b51" xlink:to="loc_us-gaap_DerivativeAssetsNoncurrent_d8222923-b0de-4afd-8c3b-001d9dc4fec4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeLiabilitiesCurrent_5666d0bc-c28c-4e2b-acf5-e3f58ab57218" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeLiabilitiesCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativesFairValueLineItems_da3eec30-ac33-48d2-b86a-601361095b51" xlink:to="loc_us-gaap_DerivativeLiabilitiesCurrent_5666d0bc-c28c-4e2b-acf5-e3f58ab57218" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeLiabilitiesNoncurrent_5e919122-7b50-4dbd-8948-d367413f61c1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeLiabilitiesNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativesFairValueLineItems_da3eec30-ac33-48d2-b86a-601361095b51" xlink:to="loc_us-gaap_DerivativeLiabilitiesNoncurrent_5e919122-7b50-4dbd-8948-d367413f61c1" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/DerivativeInstrumentsBalanceSheetOffsettingDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#DerivativeInstrumentsBalanceSheetOffsettingDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/DerivativeInstrumentsBalanceSheetOffsettingDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeLineItems_8a4074d7-409a-41ee-a8a2-eb5b006223a7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeTable_e809f817-907f-45f1-9e80-341e66522377" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DerivativeLineItems_8a4074d7-409a-41ee-a8a2-eb5b006223a7" xlink:to="loc_us-gaap_DerivativeTable_e809f817-907f-45f1-9e80-341e66522377" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeInstrumentRiskAxis_bbdc041a-fb06-42b9-ad42-e5af1178a62d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeInstrumentRiskAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeTable_e809f817-907f-45f1-9e80-341e66522377" xlink:to="loc_us-gaap_DerivativeInstrumentRiskAxis_bbdc041a-fb06-42b9-ad42-e5af1178a62d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_bbdc041a-fb06-42b9-ad42-e5af1178a62d_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_bbdc041a-fb06-42b9-ad42-e5af1178a62d" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_bbdc041a-fb06-42b9-ad42-e5af1178a62d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_5a439cab-9c85-46a9-8372-c3b23b8fa28a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_bbdc041a-fb06-42b9-ad42-e5af1178a62d" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_5a439cab-9c85-46a9-8372-c3b23b8fa28a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommodityContractMember_8be695b2-df32-40a8-b17f-e709b3a611b9" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommodityContractMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeContractTypeDomain_5a439cab-9c85-46a9-8372-c3b23b8fa28a" xlink:to="loc_us-gaap_CommodityContractMember_8be695b2-df32-40a8-b17f-e709b3a611b9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_cd331ebf-942e-4df4-b748-c7f65489ed08" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeTable_e809f817-907f-45f1-9e80-341e66522377" xlink:to="loc_dei_LegalEntityAxis_cd331ebf-942e-4df4-b748-c7f65489ed08" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_cd331ebf-942e-4df4-b748-c7f65489ed08_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_cd331ebf-942e-4df4-b748-c7f65489ed08" xlink:to="loc_dei_EntityDomain_cd331ebf-942e-4df4-b748-c7f65489ed08_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_7809e7df-0190-45e0-85da-9d20fc7c89c3" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_cd331ebf-942e-4df4-b748-c7f65489ed08" xlink:to="loc_dei_EntityDomain_7809e7df-0190-45e0-85da-9d20fc7c89c3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_137badbf-2801-438e-9afa-ec9b41c933ea" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_7809e7df-0190-45e0-85da-9d20fc7c89c3" xlink:to="loc_lnt_IplMember_137badbf-2801-438e-9afa-ec9b41c933ea" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_aa7f8655-e55e-4092-9db6-9484bff03845" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_7809e7df-0190-45e0-85da-9d20fc7c89c3" xlink:to="loc_lnt_WplMember_aa7f8655-e55e-4092-9db6-9484bff03845" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeFairValueOfDerivativeAsset_35c23b0b-dbc5-47a8-b465-aa0bbd8e62fa" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeFairValueOfDerivativeAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_8a4074d7-409a-41ee-a8a2-eb5b006223a7" xlink:to="loc_us-gaap_DerivativeFairValueOfDerivativeAsset_35c23b0b-dbc5-47a8-b465-aa0bbd8e62fa" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeFairValueOfDerivativeAssetAmountOffsetAgainstCollateral_89e7bcef-b07c-4b07-945b-18933ca7a1a3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeFairValueOfDerivativeAssetAmountOffsetAgainstCollateral"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_8a4074d7-409a-41ee-a8a2-eb5b006223a7" xlink:to="loc_us-gaap_DerivativeFairValueOfDerivativeAssetAmountOffsetAgainstCollateral_89e7bcef-b07c-4b07-945b-18933ca7a1a3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeFairValueOfDerivativeLiability_7c104069-0c6b-4537-9aa8-fd885445df7d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeFairValueOfDerivativeLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_8a4074d7-409a-41ee-a8a2-eb5b006223a7" xlink:to="loc_us-gaap_DerivativeFairValueOfDerivativeLiability_7c104069-0c6b-4537-9aa8-fd885445df7d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeFairValueOfDerivativeLiabilityAmountOffsetAgainstCollateral_71e2b681-a1f3-4ce8-9f70-5eabc6abf12a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeFairValueOfDerivativeLiabilityAmountOffsetAgainstCollateral"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_8a4074d7-409a-41ee-a8a2-eb5b006223a7" xlink:to="loc_us-gaap_DerivativeFairValueOfDerivativeLiabilityAmountOffsetAgainstCollateral_71e2b681-a1f3-4ce8-9f70-5eabc6abf12a" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/FairValueMeasurementsNarrativeDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#FairValueMeasurementsNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/FairValueMeasurementsNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_b07d2cdb-b8fb-46ee-b5db-f23725eac3c5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_dac1eda8-ac7f-431c-9c61-67be3818c55e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_b07d2cdb-b8fb-46ee-b5db-f23725eac3c5" xlink:to="loc_us-gaap_StatementTable_dac1eda8-ac7f-431c-9c61-67be3818c55e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_11f15458-f05c-4b77-b849-e20cf0c305e5" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_dac1eda8-ac7f-431c-9c61-67be3818c55e" xlink:to="loc_dei_LegalEntityAxis_11f15458-f05c-4b77-b849-e20cf0c305e5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_11f15458-f05c-4b77-b849-e20cf0c305e5_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_11f15458-f05c-4b77-b849-e20cf0c305e5" xlink:to="loc_dei_EntityDomain_11f15458-f05c-4b77-b849-e20cf0c305e5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_325f6ed2-4f18-4ac5-a0f6-7b8ba727702f" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_11f15458-f05c-4b77-b849-e20cf0c305e5" xlink:to="loc_dei_EntityDomain_325f6ed2-4f18-4ac5-a0f6-7b8ba727702f" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/FairValueMeasurementsFairValueMeasurementsDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#FairValueMeasurementsFairValueMeasurementsDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/FairValueMeasurementsFairValueMeasurementsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_e31378b6-946b-49da-95bd-279fb2f53930" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueLiabilitiesMeasuredOnRecurringBasisUnobservableInputReconciliationTable_38689a18-666a-449c-88a6-a3926c702f70" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueLiabilitiesMeasuredOnRecurringBasisUnobservableInputReconciliationTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_e31378b6-946b-49da-95bd-279fb2f53930" xlink:to="loc_us-gaap_FairValueLiabilitiesMeasuredOnRecurringBasisUnobservableInputReconciliationTable_38689a18-666a-449c-88a6-a3926c702f70" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementBasisAxis_60196104-8e23-48e7-8067-3a9a840f352f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueByMeasurementBasisAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueLiabilitiesMeasuredOnRecurringBasisUnobservableInputReconciliationTable_38689a18-666a-449c-88a6-a3926c702f70" xlink:to="loc_us-gaap_FairValueByMeasurementBasisAxis_60196104-8e23-48e7-8067-3a9a840f352f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_60196104-8e23-48e7-8067-3a9a840f352f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_60196104-8e23-48e7-8067-3a9a840f352f" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_60196104-8e23-48e7-8067-3a9a840f352f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueDisclosureItemAmountsDomain_f5318279-51a1-4855-8981-adf71f33179c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueDisclosureItemAmountsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_60196104-8e23-48e7-8067-3a9a840f352f" xlink:to="loc_us-gaap_FairValueDisclosureItemAmountsDomain_f5318279-51a1-4855-8981-adf71f33179c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CarryingReportedAmountFairValueDisclosureMember_a7bb2333-2511-4fc2-a661-b62bfba8df94" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CarryingReportedAmountFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueDisclosureItemAmountsDomain_f5318279-51a1-4855-8981-adf71f33179c" xlink:to="loc_us-gaap_CarryingReportedAmountFairValueDisclosureMember_a7bb2333-2511-4fc2-a661-b62bfba8df94" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_468b061c-6961-4037-9ae5-972093082f4e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueDisclosureItemAmountsDomain_f5318279-51a1-4855-8981-adf71f33179c" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_468b061c-6961-4037-9ae5-972093082f4e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_60461d96-e559-4764-8037-ddfdfc434d63" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueLiabilitiesMeasuredOnRecurringBasisUnobservableInputReconciliationTable_38689a18-666a-449c-88a6-a3926c702f70" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_60461d96-e559-4764-8037-ddfdfc434d63" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_60461d96-e559-4764-8037-ddfdfc434d63_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_60461d96-e559-4764-8037-ddfdfc434d63" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_60461d96-e559-4764-8037-ddfdfc434d63_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_6df1be23-e2db-4d33-ac1a-d1489fe03ab3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_60461d96-e559-4764-8037-ddfdfc434d63" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_6df1be23-e2db-4d33-ac1a-d1489fe03ab3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_526a40ef-d6e3-48e1-91c2-9fdadcf14d15" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_6df1be23-e2db-4d33-ac1a-d1489fe03ab3" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_526a40ef-d6e3-48e1-91c2-9fdadcf14d15" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_f329a478-3dab-435c-a952-68c5f2597211" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_6df1be23-e2db-4d33-ac1a-d1489fe03ab3" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_f329a478-3dab-435c-a952-68c5f2597211" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_227bd722-c8ce-4db2-a462-e99807725132" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueInputsLevel3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_6df1be23-e2db-4d33-ac1a-d1489fe03ab3" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_227bd722-c8ce-4db2-a462-e99807725132" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeInstrumentRiskAxis_cef57ad7-023d-4b2a-90b4-4ae93d0b6b80" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeInstrumentRiskAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueLiabilitiesMeasuredOnRecurringBasisUnobservableInputReconciliationTable_38689a18-666a-449c-88a6-a3926c702f70" xlink:to="loc_us-gaap_DerivativeInstrumentRiskAxis_cef57ad7-023d-4b2a-90b4-4ae93d0b6b80" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_cef57ad7-023d-4b2a-90b4-4ae93d0b6b80_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_cef57ad7-023d-4b2a-90b4-4ae93d0b6b80" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_cef57ad7-023d-4b2a-90b4-4ae93d0b6b80_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_6b2c4ec9-095f-47fe-b4d9-78130e7e6b7b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_cef57ad7-023d-4b2a-90b4-4ae93d0b6b80" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_6b2c4ec9-095f-47fe-b4d9-78130e7e6b7b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommodityContractMember_387aaef7-dbf1-40bc-a925-9e9b3558923f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommodityContractMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeContractTypeDomain_6b2c4ec9-095f-47fe-b4d9-78130e7e6b7b" xlink:to="loc_us-gaap_CommodityContractMember_387aaef7-dbf1-40bc-a925-9e9b3558923f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestRateSwapMember_775f7e57-e594-4428-8d34-4d72b8d29783" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_InterestRateSwapMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeContractTypeDomain_6b2c4ec9-095f-47fe-b4d9-78130e7e6b7b" xlink:to="loc_us-gaap_InterestRateSwapMember_775f7e57-e594-4428-8d34-4d72b8d29783" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_0867dcc3-fc9e-49f0-8a25-f92dec5aae12" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueLiabilitiesMeasuredOnRecurringBasisUnobservableInputReconciliationTable_38689a18-666a-449c-88a6-a3926c702f70" xlink:to="loc_dei_LegalEntityAxis_0867dcc3-fc9e-49f0-8a25-f92dec5aae12" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_0867dcc3-fc9e-49f0-8a25-f92dec5aae12_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_0867dcc3-fc9e-49f0-8a25-f92dec5aae12" xlink:to="loc_dei_EntityDomain_0867dcc3-fc9e-49f0-8a25-f92dec5aae12_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_17ae4f0d-c132-4413-9f15-8ee521c47124" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_0867dcc3-fc9e-49f0-8a25-f92dec5aae12" xlink:to="loc_dei_EntityDomain_17ae4f0d-c132-4413-9f15-8ee521c47124" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_d9d7b2bc-8f1f-4b44-82c6-c9fa67e5c548" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_17ae4f0d-c132-4413-9f15-8ee521c47124" xlink:to="loc_lnt_IplMember_d9d7b2bc-8f1f-4b44-82c6-c9fa67e5c548" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_02494349-fccc-40df-bfff-600c1d083a66" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_17ae4f0d-c132-4413-9f15-8ee521c47124" xlink:to="loc_lnt_WplMember_02494349-fccc-40df-bfff-600c1d083a66" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsAbstract_9f49ab98-6735-4f4b-9dd6-6159f0abaffd" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AssetsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e31378b6-946b-49da-95bd-279fb2f53930" xlink:to="loc_us-gaap_AssetsAbstract_9f49ab98-6735-4f4b-9dd6-6159f0abaffd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MoneyMarketFundsAtCarryingValue_f86bd392-ce1d-4b88-9d0e-fa75d8000e08" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_MoneyMarketFundsAtCarryingValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_9f49ab98-6735-4f4b-9dd6-6159f0abaffd" xlink:to="loc_us-gaap_MoneyMarketFundsAtCarryingValue_f86bd392-ce1d-4b88-9d0e-fa75d8000e08" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeAssets_51010af0-d4ba-45ab-9b7e-21a26e30cd85" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_9f49ab98-6735-4f4b-9dd6-6159f0abaffd" xlink:to="loc_us-gaap_DerivativeAssets_51010af0-d4ba-45ab-9b7e-21a26e30cd85" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_DeferredProceedsOfReceivablesSold_d23616b9-b62c-4e2e-a775-053ff7242ba6" xlink:href="lnt-20240331.xsd#lnt_DeferredProceedsOfReceivablesSold"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_9f49ab98-6735-4f4b-9dd6-6159f0abaffd" xlink:to="loc_lnt_DeferredProceedsOfReceivablesSold_d23616b9-b62c-4e2e-a775-053ff7242ba6" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_FairValueOfDeferredProceedsOfReceivablesSold_3176732f-e1f0-47f9-9285-4ab5733730d3" xlink:href="lnt-20240331.xsd#lnt_FairValueOfDeferredProceedsOfReceivablesSold"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_9f49ab98-6735-4f4b-9dd6-6159f0abaffd" xlink:to="loc_lnt_FairValueOfDeferredProceedsOfReceivablesSold_3176732f-e1f0-47f9-9285-4ab5733730d3" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_CapitalizationAndLiabilitiesAbstract_07114681-f57f-4d48-8e8b-7bc529d3b1ab" xlink:href="lnt-20240331.xsd#lnt_CapitalizationAndLiabilitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e31378b6-946b-49da-95bd-279fb2f53930" xlink:to="loc_lnt_CapitalizationAndLiabilitiesAbstract_07114681-f57f-4d48-8e8b-7bc529d3b1ab" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeLiabilities_81f5a12f-136a-414b-8f92-5de0f3afca53" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lnt_CapitalizationAndLiabilitiesAbstract_07114681-f57f-4d48-8e8b-7bc529d3b1ab" xlink:to="loc_us-gaap_DerivativeLiabilities_81f5a12f-136a-414b-8f92-5de0f3afca53" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_73fb697a-87f3-46f7-8873-86d15e329aa6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lnt_CapitalizationAndLiabilitiesAbstract_07114681-f57f-4d48-8e8b-7bc529d3b1ab" xlink:to="loc_us-gaap_LongTermDebt_73fb697a-87f3-46f7-8873-86d15e329aa6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtFairValue_7ca824dc-7fbd-4ee6-9139-19f6e0b454ba" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermDebtFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lnt_CapitalizationAndLiabilitiesAbstract_07114681-f57f-4d48-8e8b-7bc529d3b1ab" xlink:to="loc_us-gaap_LongTermDebtFairValue_7ca824dc-7fbd-4ee6-9139-19f6e0b454ba" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/FairValueMeasurementsFairValueMeasurementsUsingSignificantUnobservableInputsDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#FairValueMeasurementsFairValueMeasurementsUsingSignificantUnobservableInputsDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/FairValueMeasurementsFairValueMeasurementsUsingSignificantUnobservableInputsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_5912076c-8df3-4275-9b65-fd26dcbd56c7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationTable_613ba38d-4540-41bc-9188-1ac38b2ab88d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_5912076c-8df3-4275-9b65-fd26dcbd56c7" xlink:to="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationTable_613ba38d-4540-41bc-9188-1ac38b2ab88d" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByAssetClassAxis_0f5a6e87-2248-472e-904a-6d422a8a35c9" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueByAssetClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationTable_613ba38d-4540-41bc-9188-1ac38b2ab88d" xlink:to="loc_us-gaap_FairValueByAssetClassAxis_0f5a6e87-2248-472e-904a-6d422a8a35c9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_0f5a6e87-2248-472e-904a-6d422a8a35c9_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByAssetClassAxis_0f5a6e87-2248-472e-904a-6d422a8a35c9" xlink:to="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_0f5a6e87-2248-472e-904a-6d422a8a35c9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_c7fc4f82-0cea-4516-9039-616fa18b1725" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByAssetClassAxis_0f5a6e87-2248-472e-904a-6d422a8a35c9" xlink:to="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_c7fc4f82-0cea-4516-9039-616fa18b1725" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommodityContractMember_44eafd78-aa9d-4426-aec2-5b1a598e0fc4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommodityContractMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_c7fc4f82-0cea-4516-9039-616fa18b1725" xlink:to="loc_us-gaap_CommodityContractMember_44eafd78-aa9d-4426-aec2-5b1a598e0fc4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_DeferredProceedsOfReceivablesSoldMember_6db01879-ff89-49e3-8bfd-515d207db01e" xlink:href="lnt-20240331.xsd#lnt_DeferredProceedsOfReceivablesSoldMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_c7fc4f82-0cea-4516-9039-616fa18b1725" xlink:to="loc_lnt_DeferredProceedsOfReceivablesSoldMember_6db01879-ff89-49e3-8bfd-515d207db01e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_7e14eb39-3488-476b-933e-abf74ae551fd" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationTable_613ba38d-4540-41bc-9188-1ac38b2ab88d" xlink:to="loc_dei_LegalEntityAxis_7e14eb39-3488-476b-933e-abf74ae551fd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_7e14eb39-3488-476b-933e-abf74ae551fd_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_7e14eb39-3488-476b-933e-abf74ae551fd" xlink:to="loc_dei_EntityDomain_7e14eb39-3488-476b-933e-abf74ae551fd_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_3be1ee2a-fbbe-4531-bd10-ba068162a882" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_7e14eb39-3488-476b-933e-abf74ae551fd" xlink:to="loc_dei_EntityDomain_3be1ee2a-fbbe-4531-bd10-ba068162a882" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_15abf9f4-0d18-4f63-89c5-dd0949469d23" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_3be1ee2a-fbbe-4531-bd10-ba068162a882" xlink:to="loc_lnt_IplMember_15abf9f4-0d18-4f63-89c5-dd0949469d23" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_00e0b6d8-e8c0-453f-bf01-b84c92acbd17" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_3be1ee2a-fbbe-4531-bd10-ba068162a882" xlink:to="loc_lnt_WplMember_00e0b6d8-e8c0-453f-bf01-b84c92acbd17" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueNetDerivativeAssetLiabilityMeasuredOnRecurringBasisUnobservableInputReconciliationRollForward_760f8fe0-9ed0-4847-8924-7300427c6751" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueNetDerivativeAssetLiabilityMeasuredOnRecurringBasisUnobservableInputReconciliationRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_5912076c-8df3-4275-9b65-fd26dcbd56c7" xlink:to="loc_us-gaap_FairValueNetDerivativeAssetLiabilityMeasuredOnRecurringBasisUnobservableInputReconciliationRollForward_760f8fe0-9ed0-4847-8924-7300427c6751" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationRecurringBasisAssetValue_cc6540c7-ea2d-41d0-80ba-797e9a227858" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationRecurringBasisAssetValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueNetDerivativeAssetLiabilityMeasuredOnRecurringBasisUnobservableInputReconciliationRollForward_760f8fe0-9ed0-4847-8924-7300427c6751" xlink:to="loc_us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationRecurringBasisAssetValue_cc6540c7-ea2d-41d0-80ba-797e9a227858" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationRecurringBasisAssetGainLossIncludedInEarnings1_4a85a268-6492-4ed4-9931-8028431f77eb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationRecurringBasisAssetGainLossIncludedInEarnings1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueNetDerivativeAssetLiabilityMeasuredOnRecurringBasisUnobservableInputReconciliationRollForward_760f8fe0-9ed0-4847-8924-7300427c6751" xlink:to="loc_us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationRecurringBasisAssetGainLossIncludedInEarnings1_4a85a268-6492-4ed4-9931-8028431f77eb" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationRecurringBasisAssetSettlements_3b9bee9b-bc75-4d67-b5c8-2a4b2cdf541a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationRecurringBasisAssetSettlements"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueNetDerivativeAssetLiabilityMeasuredOnRecurringBasisUnobservableInputReconciliationRollForward_760f8fe0-9ed0-4847-8924-7300427c6751" xlink:to="loc_us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationRecurringBasisAssetSettlements_3b9bee9b-bc75-4d67-b5c8-2a4b2cdf541a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationRecurringBasisAssetValue_ff5e00e1-cf72-4572-9381-f87988bff0ad" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationRecurringBasisAssetValue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisChangeInUnrealizedGainLoss_8ffa783d-2ab5-4df7-8f1f-60e4acf98803" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueAssetsMeasuredOnRecurringBasisChangeInUnrealizedGainLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_5912076c-8df3-4275-9b65-fd26dcbd56c7" xlink:to="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisChangeInUnrealizedGainLoss_8ffa783d-2ab5-4df7-8f1f-60e4acf98803" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/FairValueMeasurementsFairValueOfNetDerivativeAssetsLiabilitiesDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#FairValueMeasurementsFairValueOfNetDerivativeAssetsLiabilitiesDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/FairValueMeasurementsFairValueOfNetDerivativeAssetsLiabilitiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationLineItems_c41c5b6f-b763-4460-9858-1f9ff5e8e76e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationTable_f4aa0030-dd45-4ae8-993d-07ccf873afe3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationLineItems_c41c5b6f-b763-4460-9858-1f9ff5e8e76e" xlink:to="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationTable_f4aa0030-dd45-4ae8-993d-07ccf873afe3" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_bb523dca-c2ad-4aab-8ed9-431a4997aa8d" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationTable_f4aa0030-dd45-4ae8-993d-07ccf873afe3" xlink:to="loc_dei_LegalEntityAxis_bb523dca-c2ad-4aab-8ed9-431a4997aa8d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_bb523dca-c2ad-4aab-8ed9-431a4997aa8d_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_bb523dca-c2ad-4aab-8ed9-431a4997aa8d" xlink:to="loc_dei_EntityDomain_bb523dca-c2ad-4aab-8ed9-431a4997aa8d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_0683f2e8-fbd2-4102-b391-3cc94c73abdf" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_bb523dca-c2ad-4aab-8ed9-431a4997aa8d" xlink:to="loc_dei_EntityDomain_0683f2e8-fbd2-4102-b391-3cc94c73abdf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_01266e82-8384-4432-9b17-d4be7b29fedf" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_0683f2e8-fbd2-4102-b391-3cc94c73abdf" xlink:to="loc_lnt_IplMember_01266e82-8384-4432-9b17-d4be7b29fedf" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_5ac09775-9adc-450c-ae11-16c55b937a4e" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_0683f2e8-fbd2-4102-b391-3cc94c73abdf" xlink:to="loc_lnt_WplMember_5ac09775-9adc-450c-ae11-16c55b937a4e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_EnergyAxis_4abcf2f4-f350-42d5-b202-53d4ae73417e" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_EnergyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationTable_f4aa0030-dd45-4ae8-993d-07ccf873afe3" xlink:to="loc_srt_EnergyAxis_4abcf2f4-f350-42d5-b202-53d4ae73417e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_EnergyDomain_4abcf2f4-f350-42d5-b202-53d4ae73417e_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_EnergyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_EnergyAxis_4abcf2f4-f350-42d5-b202-53d4ae73417e" xlink:to="loc_srt_EnergyDomain_4abcf2f4-f350-42d5-b202-53d4ae73417e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_EnergyDomain_9aa8815c-86e1-4836-96d4-24e6e191c61d" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_EnergyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_EnergyAxis_4abcf2f4-f350-42d5-b202-53d4ae73417e" xlink:to="loc_srt_EnergyDomain_9aa8815c-86e1-4836-96d4-24e6e191c61d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_ExcludingFinancialTransmissionRightsMember_aea57546-f886-4044-9e20-286a45e8233e" xlink:href="lnt-20240331.xsd#lnt_ExcludingFinancialTransmissionRightsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_EnergyDomain_9aa8815c-86e1-4836-96d4-24e6e191c61d" xlink:to="loc_lnt_ExcludingFinancialTransmissionRightsMember_aea57546-f886-4044-9e20-286a45e8233e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_FinancialTransmissionRightsMember_85a6d1df-70b3-4343-a6dd-90189e985970" xlink:href="lnt-20240331.xsd#lnt_FinancialTransmissionRightsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_EnergyDomain_9aa8815c-86e1-4836-96d4-24e6e191c61d" xlink:to="loc_lnt_FinancialTransmissionRightsMember_85a6d1df-70b3-4343-a6dd-90189e985970" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByAssetClassAxis_5e19205b-4c31-444d-8ed8-f708742b7464" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueByAssetClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationTable_f4aa0030-dd45-4ae8-993d-07ccf873afe3" xlink:to="loc_us-gaap_FairValueByAssetClassAxis_5e19205b-4c31-444d-8ed8-f708742b7464" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_5e19205b-4c31-444d-8ed8-f708742b7464_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByAssetClassAxis_5e19205b-4c31-444d-8ed8-f708742b7464" xlink:to="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_5e19205b-4c31-444d-8ed8-f708742b7464_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_3dc39e63-cff5-437d-af95-3f59cc1c84b4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByAssetClassAxis_5e19205b-4c31-444d-8ed8-f708742b7464" xlink:to="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_3dc39e63-cff5-437d-af95-3f59cc1c84b4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommodityContractMember_ed052d52-6aca-4533-9129-bec59b067297" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommodityContractMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_3dc39e63-cff5-437d-af95-3f59cc1c84b4" xlink:to="loc_us-gaap_CommodityContractMember_ed052d52-6aca-4533-9129-bec59b067297" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationRecurringBasisAssetValue_e1a26673-7190-4267-8619-13a352440549" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationRecurringBasisAssetValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationLineItems_c41c5b6f-b763-4460-9858-1f9ff5e8e76e" xlink:to="loc_us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationRecurringBasisAssetValue_e1a26673-7190-4267-8619-13a352440549" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationsRecurringBasisLiabilityValue_15c2d2aa-159b-4521-b51b-586f8ff6ac72" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationsRecurringBasisLiabilityValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationLineItems_c41c5b6f-b763-4460-9858-1f9ff5e8e76e" xlink:to="loc_us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationsRecurringBasisLiabilityValue_15c2d2aa-159b-4521-b51b-586f8ff6ac72" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/CommitmentsAndContingenciesNarrativeDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#CommitmentsAndContingenciesNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/CommitmentsAndContingenciesNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermPurchaseCommitmentLineItems_6282a12f-3c10-45bc-846b-8f245df99f36" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermPurchaseCommitmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermPurchaseCommitmentTable_30a8d157-4188-462c-a803-c4dd8affb39d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermPurchaseCommitmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentLineItems_6282a12f-3c10-45bc-846b-8f245df99f36" xlink:to="loc_us-gaap_LongTermPurchaseCommitmentTable_30a8d157-4188-462c-a803-c4dd8affb39d" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryAgencyAxis_67152722-f33e-40f3-a695-55f61f04de61" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatoryAgencyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentTable_30a8d157-4188-462c-a803-c4dd8affb39d" xlink:to="loc_us-gaap_RegulatoryAgencyAxis_67152722-f33e-40f3-a695-55f61f04de61" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryAgencyDomain_67152722-f33e-40f3-a695-55f61f04de61_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatoryAgencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RegulatoryAgencyAxis_67152722-f33e-40f3-a695-55f61f04de61" xlink:to="loc_us-gaap_RegulatoryAgencyDomain_67152722-f33e-40f3-a695-55f61f04de61_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryAgencyDomain_16d16f61-92bc-4649-af8b-99ebb989372e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatoryAgencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RegulatoryAgencyAxis_67152722-f33e-40f3-a695-55f61f04de61" xlink:to="loc_us-gaap_RegulatoryAgencyDomain_16d16f61-92bc-4649-af8b-99ebb989372e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_FederalEnergyRegulatoryCommissionMember_d4364d80-be60-4d1a-86d4-7057794a8fb6" xlink:href="lnt-20240331.xsd#lnt_FederalEnergyRegulatoryCommissionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryAgencyDomain_16d16f61-92bc-4649-af8b-99ebb989372e" xlink:to="loc_lnt_FederalEnergyRegulatoryCommissionMember_d4364d80-be60-4d1a-86d4-7057794a8fb6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_OwnershipAxis_a57d03cc-9e6d-4433-bd3f-de6857fdb881" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_OwnershipAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentTable_30a8d157-4188-462c-a803-c4dd8affb39d" xlink:to="loc_srt_OwnershipAxis_a57d03cc-9e6d-4433-bd3f-de6857fdb881" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_OwnershipDomain_a57d03cc-9e6d-4433-bd3f-de6857fdb881_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_OwnershipDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_OwnershipAxis_a57d03cc-9e6d-4433-bd3f-de6857fdb881" xlink:to="loc_srt_OwnershipDomain_a57d03cc-9e6d-4433-bd3f-de6857fdb881_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_OwnershipDomain_94fe5d42-3b6c-4a1a-8e3e-444ea2c2c227" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_OwnershipDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_OwnershipAxis_a57d03cc-9e6d-4433-bd3f-de6857fdb881" xlink:to="loc_srt_OwnershipDomain_94fe5d42-3b6c-4a1a-8e3e-444ea2c2c227" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WhitingPetroleumCorporationMember_b13e50a4-6150-4b6d-a265-3f0706be5091" xlink:href="lnt-20240331.xsd#lnt_WhitingPetroleumCorporationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_OwnershipDomain_94fe5d42-3b6c-4a1a-8e3e-444ea2c2c227" xlink:to="loc_lnt_WhitingPetroleumCorporationMember_b13e50a4-6150-4b6d-a265-3f0706be5091" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_1e66fa25-f68e-42cb-8d1a-bdbd22e3f999" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskByBenchmarkAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentTable_30a8d157-4188-462c-a803-c4dd8affb39d" xlink:to="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_1e66fa25-f68e-42cb-8d1a-bdbd22e3f999" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_1e66fa25-f68e-42cb-8d1a-bdbd22e3f999_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_1e66fa25-f68e-42cb-8d1a-bdbd22e3f999" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_1e66fa25-f68e-42cb-8d1a-bdbd22e3f999_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_063fab3e-e97a-41e6-98ea-ace2f1ebca19" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_1e66fa25-f68e-42cb-8d1a-bdbd22e3f999" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_063fab3e-e97a-41e6-98ea-ace2f1ebca19" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WorkforceSubjectToCollectiveBargainingArrangementsMember_9341da37-bdb5-413d-936a-90df5d74cd69" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_WorkforceSubjectToCollectiveBargainingArrangementsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskBenchmarkDomain_063fab3e-e97a-41e6-98ea-ace2f1ebca19" xlink:to="loc_us-gaap_WorkforceSubjectToCollectiveBargainingArrangementsMember_9341da37-bdb5-413d-936a-90df5d74cd69" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WorkforceSubjectToCollectiveBargainingArrangementsExpiringWithinOneYearMember_95985df0-a93c-4993-9b6b-9aa1ded0c3e9" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_WorkforceSubjectToCollectiveBargainingArrangementsExpiringWithinOneYearMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskBenchmarkDomain_063fab3e-e97a-41e6-98ea-ace2f1ebca19" xlink:to="loc_us-gaap_WorkforceSubjectToCollectiveBargainingArrangementsExpiringWithinOneYearMember_95985df0-a93c-4993-9b6b-9aa1ded0c3e9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GuaranteeObligationsByNatureAxis_e4836185-919b-4a33-8436-978faf2c0e59" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_GuaranteeObligationsByNatureAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentTable_30a8d157-4188-462c-a803-c4dd8affb39d" xlink:to="loc_us-gaap_GuaranteeObligationsByNatureAxis_e4836185-919b-4a33-8436-978faf2c0e59" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GuaranteeObligationsNatureDomain_e4836185-919b-4a33-8436-978faf2c0e59_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_GuaranteeObligationsNatureDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_GuaranteeObligationsByNatureAxis_e4836185-919b-4a33-8436-978faf2c0e59" xlink:to="loc_us-gaap_GuaranteeObligationsNatureDomain_e4836185-919b-4a33-8436-978faf2c0e59_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GuaranteeObligationsNatureDomain_db528660-e5ff-42b3-a9a4-814ae84dc82a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_GuaranteeObligationsNatureDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_GuaranteeObligationsByNatureAxis_e4836185-919b-4a33-8436-978faf2c0e59" xlink:to="loc_us-gaap_GuaranteeObligationsNatureDomain_db528660-e5ff-42b3-a9a4-814ae84dc82a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IndemnificationGuaranteeMember_5cec0d68-3964-4c7f-955c-51787fcbcca5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IndemnificationGuaranteeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_GuaranteeObligationsNatureDomain_db528660-e5ff-42b3-a9a4-814ae84dc82a" xlink:to="loc_us-gaap_IndemnificationGuaranteeMember_5cec0d68-3964-4c7f-955c-51787fcbcca5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermPurchaseCommitmentByCategoryOfItemPurchasedAxis_c9ffd26e-75f5-438e-9659-596b2433cb22" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermPurchaseCommitmentByCategoryOfItemPurchasedAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentTable_30a8d157-4188-462c-a803-c4dd8affb39d" xlink:to="loc_us-gaap_LongTermPurchaseCommitmentByCategoryOfItemPurchasedAxis_c9ffd26e-75f5-438e-9659-596b2433cb22" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermPurchaseCommitmentCategoryOfItemPurchasedDomain_c9ffd26e-75f5-438e-9659-596b2433cb22_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermPurchaseCommitmentCategoryOfItemPurchasedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentByCategoryOfItemPurchasedAxis_c9ffd26e-75f5-438e-9659-596b2433cb22" xlink:to="loc_us-gaap_LongTermPurchaseCommitmentCategoryOfItemPurchasedDomain_c9ffd26e-75f5-438e-9659-596b2433cb22_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermPurchaseCommitmentCategoryOfItemPurchasedDomain_64418ae0-571b-4b8b-8172-4651213ca351" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermPurchaseCommitmentCategoryOfItemPurchasedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentByCategoryOfItemPurchasedAxis_c9ffd26e-75f5-438e-9659-596b2433cb22" xlink:to="loc_us-gaap_LongTermPurchaseCommitmentCategoryOfItemPurchasedDomain_64418ae0-571b-4b8b-8172-4651213ca351" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_CapitalPurchaseObligationMember_c1f28e9e-e118-40c5-8fc3-5302ae7a3305" xlink:href="lnt-20240331.xsd#lnt_CapitalPurchaseObligationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentCategoryOfItemPurchasedDomain_64418ae0-571b-4b8b-8172-4651213ca351" xlink:to="loc_lnt_CapitalPurchaseObligationMember_c1f28e9e-e118-40c5-8fc3-5302ae7a3305" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_PurchasedPowerMember_ada5a975-8817-4ffc-8f0e-af219e7b346c" xlink:href="lnt-20240331.xsd#lnt_PurchasedPowerMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentCategoryOfItemPurchasedDomain_64418ae0-571b-4b8b-8172-4651213ca351" xlink:to="loc_lnt_PurchasedPowerMember_ada5a975-8817-4ffc-8f0e-af219e7b346c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_RenewableTaxCreditsTransferredMember_1abe9d3e-c49c-463c-92c2-ae1873e894a6" xlink:href="lnt-20240331.xsd#lnt_RenewableTaxCreditsTransferredMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentCategoryOfItemPurchasedDomain_64418ae0-571b-4b8b-8172-4651213ca351" xlink:to="loc_lnt_RenewableTaxCreditsTransferredMember_1abe9d3e-c49c-463c-92c2-ae1873e894a6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_d31b788f-bc3e-4c73-88f1-15a828e71a4d" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentTable_30a8d157-4188-462c-a803-c4dd8affb39d" xlink:to="loc_dei_LegalEntityAxis_d31b788f-bc3e-4c73-88f1-15a828e71a4d" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_d31b788f-bc3e-4c73-88f1-15a828e71a4d_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_d31b788f-bc3e-4c73-88f1-15a828e71a4d" xlink:to="loc_dei_EntityDomain_d31b788f-bc3e-4c73-88f1-15a828e71a4d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_2bca75ff-589c-49d5-80f8-e3b2eb53f362" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_d31b788f-bc3e-4c73-88f1-15a828e71a4d" xlink:to="loc_dei_EntityDomain_2bca75ff-589c-49d5-80f8-e3b2eb53f362" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_976b9ebe-8634-434f-9c14-4f80021b302f" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_2bca75ff-589c-49d5-80f8-e3b2eb53f362" xlink:to="loc_lnt_IplMember_976b9ebe-8634-434f-9c14-4f80021b302f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_ee275e55-060b-496e-b9f9-07c595c658dd" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_2bca75ff-589c-49d5-80f8-e3b2eb53f362" xlink:to="loc_lnt_WplMember_ee275e55-060b-496e-b9f9-07c595c658dd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermPurchaseCommitmentAmount_f9d3cacd-9bc4-45f3-8161-37081adc4e7f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermPurchaseCommitmentAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentLineItems_6282a12f-3c10-45bc-846b-8f245df99f36" xlink:to="loc_us-gaap_LongTermPurchaseCommitmentAmount_f9d3cacd-9bc4-45f3-8161-37081adc4e7f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LimitedLiabilityCompanyLLCOrLimitedPartnershipLPMembersOrLimitedPartnersOwnershipInterest_a0241c20-a7b1-4d7a-848a-8c5387a6cdb8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LimitedLiabilityCompanyLLCOrLimitedPartnershipLPMembersOrLimitedPartnersOwnershipInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentLineItems_6282a12f-3c10-45bc-846b-8f245df99f36" xlink:to="loc_us-gaap_LimitedLiabilityCompanyLLCOrLimitedPartnershipLPMembersOrLimitedPartnersOwnershipInterest_a0241c20-a7b1-4d7a-848a-8c5387a6cdb8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GuaranteeObligationsMaximumExposure_6f7b96ea-24d8-402f-a619-76c83d4a3abc" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_GuaranteeObligationsMaximumExposure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentLineItems_6282a12f-3c10-45bc-846b-8f245df99f36" xlink:to="loc_us-gaap_GuaranteeObligationsMaximumExposure_6f7b96ea-24d8-402f-a619-76c83d4a3abc" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GuaranteeObligationsLiquidationProceeds_a2db170c-ce64-4572-9648-8f2f088ff091" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_GuaranteeObligationsLiquidationProceeds"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentLineItems_6282a12f-3c10-45bc-846b-8f245df99f36" xlink:to="loc_us-gaap_GuaranteeObligationsLiquidationProceeds_a2db170c-ce64-4572-9648-8f2f088ff091" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PublicUtilitiesApprovedReturnOnEquityPercentage_528cd4ad-b9b8-44de-a6f1-9e46007f24bd" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PublicUtilitiesApprovedReturnOnEquityPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentLineItems_6282a12f-3c10-45bc-846b-8f245df99f36" xlink:to="loc_us-gaap_PublicUtilitiesApprovedReturnOnEquityPercentage_528cd4ad-b9b8-44de-a6f1-9e46007f24bd" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_ConcentrationRiskThresholdPercentage_7a6d0309-e380-4638-b334-ee52a16463c4" xlink:href="lnt-20240331.xsd#lnt_ConcentrationRiskThresholdPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentLineItems_6282a12f-3c10-45bc-846b-8f245df99f36" xlink:to="loc_lnt_ConcentrationRiskThresholdPercentage_7a6d0309-e380-4638-b334-ee52a16463c4" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/CommitmentsAndContingenciesOtherPurchaseCommitmentsDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#CommitmentsAndContingenciesOtherPurchaseCommitmentsDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/CommitmentsAndContingenciesOtherPurchaseCommitmentsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermPurchaseCommitmentLineItems_e0d54f7c-f8bd-4d03-98a0-234bac7cfa16" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermPurchaseCommitmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermPurchaseCommitmentTable_101f2cce-9a1b-4665-aa92-700849010b83" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermPurchaseCommitmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentLineItems_e0d54f7c-f8bd-4d03-98a0-234bac7cfa16" xlink:to="loc_us-gaap_LongTermPurchaseCommitmentTable_101f2cce-9a1b-4665-aa92-700849010b83" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementScenarioAxis_1b9c74cb-49c1-43ab-bae0-8b2be9477dfc" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_StatementScenarioAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentTable_101f2cce-9a1b-4665-aa92-700849010b83" xlink:to="loc_srt_StatementScenarioAxis_1b9c74cb-49c1-43ab-bae0-8b2be9477dfc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_1b9c74cb-49c1-43ab-bae0-8b2be9477dfc_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementScenarioAxis_1b9c74cb-49c1-43ab-bae0-8b2be9477dfc" xlink:to="loc_srt_ScenarioUnspecifiedDomain_1b9c74cb-49c1-43ab-bae0-8b2be9477dfc_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_10392b74-34ba-49ca-9931-0415b72c2731" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementScenarioAxis_1b9c74cb-49c1-43ab-bae0-8b2be9477dfc" xlink:to="loc_srt_ScenarioUnspecifiedDomain_10392b74-34ba-49ca-9931-0415b72c2731" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermContractForPurchaseOfElectricPowerAxis_01b07d25-8575-42ed-b932-5493d4a39658" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermContractForPurchaseOfElectricPowerAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentTable_101f2cce-9a1b-4665-aa92-700849010b83" xlink:to="loc_us-gaap_LongTermContractForPurchaseOfElectricPowerAxis_01b07d25-8575-42ed-b932-5493d4a39658" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermContractForPurchaseOfElectricPowerDomain_01b07d25-8575-42ed-b932-5493d4a39658_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermContractForPurchaseOfElectricPowerDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongTermContractForPurchaseOfElectricPowerAxis_01b07d25-8575-42ed-b932-5493d4a39658" xlink:to="loc_us-gaap_LongTermContractForPurchaseOfElectricPowerDomain_01b07d25-8575-42ed-b932-5493d4a39658_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermContractForPurchaseOfElectricPowerDomain_164c9719-52b9-49d2-866d-8be3f0c5534d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermContractForPurchaseOfElectricPowerDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongTermContractForPurchaseOfElectricPowerAxis_01b07d25-8575-42ed-b932-5493d4a39658" xlink:to="loc_us-gaap_LongTermContractForPurchaseOfElectricPowerDomain_164c9719-52b9-49d2-866d-8be3f0c5534d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermPurchaseCommitmentByCategoryOfItemPurchasedAxis_87712923-5c35-451f-bf04-7f3bd6433e46" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermPurchaseCommitmentByCategoryOfItemPurchasedAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentTable_101f2cce-9a1b-4665-aa92-700849010b83" xlink:to="loc_us-gaap_LongTermPurchaseCommitmentByCategoryOfItemPurchasedAxis_87712923-5c35-451f-bf04-7f3bd6433e46" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermPurchaseCommitmentCategoryOfItemPurchasedDomain_87712923-5c35-451f-bf04-7f3bd6433e46_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermPurchaseCommitmentCategoryOfItemPurchasedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentByCategoryOfItemPurchasedAxis_87712923-5c35-451f-bf04-7f3bd6433e46" xlink:to="loc_us-gaap_LongTermPurchaseCommitmentCategoryOfItemPurchasedDomain_87712923-5c35-451f-bf04-7f3bd6433e46_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermPurchaseCommitmentCategoryOfItemPurchasedDomain_c7ed013f-8792-4a11-8eec-2db374b7f3ee" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermPurchaseCommitmentCategoryOfItemPurchasedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentByCategoryOfItemPurchasedAxis_87712923-5c35-451f-bf04-7f3bd6433e46" xlink:to="loc_us-gaap_LongTermPurchaseCommitmentCategoryOfItemPurchasedDomain_c7ed013f-8792-4a11-8eec-2db374b7f3ee" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PublicUtilitiesInventoryNaturalGasMember_3b9f6bcc-e2ff-43d3-b1e4-536795d132f0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PublicUtilitiesInventoryNaturalGasMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentCategoryOfItemPurchasedDomain_c7ed013f-8792-4a11-8eec-2db374b7f3ee" xlink:to="loc_us-gaap_PublicUtilitiesInventoryNaturalGasMember_3b9f6bcc-e2ff-43d3-b1e4-536795d132f0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CoalSupplyAgreementsMember_ee905637-88c4-416c-938d-97719a1b1d38" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CoalSupplyAgreementsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentCategoryOfItemPurchasedDomain_c7ed013f-8792-4a11-8eec-2db374b7f3ee" xlink:to="loc_us-gaap_CoalSupplyAgreementsMember_ee905637-88c4-416c-938d-97719a1b1d38" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_LongTermPurchaseCommitmentsOtherMember_39727e49-6872-4546-bb5d-5325d5f2117f" xlink:href="lnt-20240331.xsd#lnt_LongTermPurchaseCommitmentsOtherMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentCategoryOfItemPurchasedDomain_c7ed013f-8792-4a11-8eec-2db374b7f3ee" xlink:to="loc_lnt_LongTermPurchaseCommitmentsOtherMember_39727e49-6872-4546-bb5d-5325d5f2117f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_e5a3d583-7abb-40b8-b454-15bec5b44fc1" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentTable_101f2cce-9a1b-4665-aa92-700849010b83" xlink:to="loc_dei_LegalEntityAxis_e5a3d583-7abb-40b8-b454-15bec5b44fc1" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_e5a3d583-7abb-40b8-b454-15bec5b44fc1_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_e5a3d583-7abb-40b8-b454-15bec5b44fc1" xlink:to="loc_dei_EntityDomain_e5a3d583-7abb-40b8-b454-15bec5b44fc1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_2b1d808d-c0cf-4847-b481-b95165465e10" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_e5a3d583-7abb-40b8-b454-15bec5b44fc1" xlink:to="loc_dei_EntityDomain_2b1d808d-c0cf-4847-b481-b95165465e10" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_b2b491f7-d15d-4c8a-9ee3-8f3a829afa54" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_2b1d808d-c0cf-4847-b481-b95165465e10" xlink:to="loc_lnt_IplMember_b2b491f7-d15d-4c8a-9ee3-8f3a829afa54" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_5b90310e-bebf-459e-bc0b-3df306adb12d" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_2b1d808d-c0cf-4847-b481-b95165465e10" xlink:to="loc_lnt_WplMember_5b90310e-bebf-459e-bc0b-3df306adb12d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermPurchaseCommitmentAmount_9a9a3de8-6577-4830-8122-d39e91fd14ba" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermPurchaseCommitmentAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentLineItems_e0d54f7c-f8bd-4d03-98a0-234bac7cfa16" xlink:to="loc_us-gaap_LongTermPurchaseCommitmentAmount_9a9a3de8-6577-4830-8122-d39e91fd14ba" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IndividualCommitmentsIncurred_67d4d02f-3759-4226-9fca-ff73f1d5faed" xlink:href="lnt-20240331.xsd#lnt_IndividualCommitmentsIncurred"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentLineItems_e0d54f7c-f8bd-4d03-98a0-234bac7cfa16" xlink:to="loc_lnt_IndividualCommitmentsIncurred_67d4d02f-3759-4226-9fca-ff73f1d5faed" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/CommitmentsAndContingenciesMPGSiteEstimatedFutureCostsAndRecordedLiabilitiesDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#CommitmentsAndContingenciesMPGSiteEstimatedFutureCostsAndRecordedLiabilitiesDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/CommitmentsAndContingenciesMPGSiteEstimatedFutureCostsAndRecordedLiabilitiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermPurchaseCommitmentLineItems_049c5ff5-26f3-4885-8638-bc8ff2cf4de2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermPurchaseCommitmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermPurchaseCommitmentTable_488acaf7-37e4-4e77-800c-41dfdde72b3a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermPurchaseCommitmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentLineItems_049c5ff5-26f3-4885-8638-bc8ff2cf4de2" xlink:to="loc_us-gaap_LongTermPurchaseCommitmentTable_488acaf7-37e4-4e77-800c-41dfdde72b3a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PublicUtilityAxis_fa5446e0-4332-43b6-ae3a-bcccb36bf78e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PublicUtilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentTable_488acaf7-37e4-4e77-800c-41dfdde72b3a" xlink:to="loc_us-gaap_PublicUtilityAxis_fa5446e0-4332-43b6-ae3a-bcccb36bf78e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UtilityPlantDomain_fa5446e0-4332-43b6-ae3a-bcccb36bf78e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_UtilityPlantDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PublicUtilityAxis_fa5446e0-4332-43b6-ae3a-bcccb36bf78e" xlink:to="loc_us-gaap_UtilityPlantDomain_fa5446e0-4332-43b6-ae3a-bcccb36bf78e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UtilityPlantDomain_2df31a7b-85aa-4765-88cb-60affc938356" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_UtilityPlantDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PublicUtilityAxis_fa5446e0-4332-43b6-ae3a-bcccb36bf78e" xlink:to="loc_us-gaap_UtilityPlantDomain_2df31a7b-85aa-4765-88cb-60affc938356" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NaturalGasProcessingPlantMember_f9b74a25-9ecc-455c-9a41-2cf295074251" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NaturalGasProcessingPlantMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_UtilityPlantDomain_2df31a7b-85aa-4765-88cb-60affc938356" xlink:to="loc_us-gaap_NaturalGasProcessingPlantMember_f9b74a25-9ecc-455c-9a41-2cf295074251" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_3870a553-1f2b-43b4-bf90-23c6c7291061" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentTable_488acaf7-37e4-4e77-800c-41dfdde72b3a" xlink:to="loc_srt_RangeAxis_3870a553-1f2b-43b4-bf90-23c6c7291061" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_3870a553-1f2b-43b4-bf90-23c6c7291061_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_3870a553-1f2b-43b4-bf90-23c6c7291061" xlink:to="loc_srt_RangeMember_3870a553-1f2b-43b4-bf90-23c6c7291061_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_5e48ca32-6bab-4a3c-b85d-ef8d00626156" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_3870a553-1f2b-43b4-bf90-23c6c7291061" xlink:to="loc_srt_RangeMember_5e48ca32-6bab-4a3c-b85d-ef8d00626156" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_6c1b4721-f79d-496b-9f06-afffda5fe059" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_5e48ca32-6bab-4a3c-b85d-ef8d00626156" xlink:to="loc_srt_MinimumMember_6c1b4721-f79d-496b-9f06-afffda5fe059" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_9ed0f5f2-578c-482c-9f09-b53ab3279ba3" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_5e48ca32-6bab-4a3c-b85d-ef8d00626156" xlink:to="loc_srt_MaximumMember_9ed0f5f2-578c-482c-9f09-b53ab3279ba3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_e3c5affa-1b17-4933-a467-f9728529f366" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentTable_488acaf7-37e4-4e77-800c-41dfdde72b3a" xlink:to="loc_dei_LegalEntityAxis_e3c5affa-1b17-4933-a467-f9728529f366" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_e3c5affa-1b17-4933-a467-f9728529f366_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_e3c5affa-1b17-4933-a467-f9728529f366" xlink:to="loc_dei_EntityDomain_e3c5affa-1b17-4933-a467-f9728529f366_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_26b2ea94-5166-46dc-bd35-c7821bdb6543" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_e3c5affa-1b17-4933-a467-f9728529f366" xlink:to="loc_dei_EntityDomain_26b2ea94-5166-46dc-bd35-c7821bdb6543" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_8fa41458-b104-4a32-8196-9ccc9dfc8b23" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_26b2ea94-5166-46dc-bd35-c7821bdb6543" xlink:to="loc_lnt_IplMember_8fa41458-b104-4a32-8196-9ccc9dfc8b23" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_a7c3612c-a73a-4a2e-a67d-e018a3a87883" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_26b2ea94-5166-46dc-bd35-c7821bdb6543" xlink:to="loc_lnt_WplMember_a7c3612c-a73a-4a2e-a67d-e018a3a87883" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EnvironmentalExitCostsAnticipatedCost_05466fa6-c005-47ff-b288-e12e28a81967" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EnvironmentalExitCostsAnticipatedCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentLineItems_049c5ff5-26f3-4885-8638-bc8ff2cf4de2" xlink:to="loc_us-gaap_EnvironmentalExitCostsAnticipatedCost_05466fa6-c005-47ff-b288-e12e28a81967" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccrualForEnvironmentalLossContingencies_6d478d7e-9944-4ea6-9331-ceb7f87fe79e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccrualForEnvironmentalLossContingencies"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongTermPurchaseCommitmentLineItems_049c5ff5-26f3-4885-8638-bc8ff2cf4de2" xlink:to="loc_us-gaap_AccrualForEnvironmentalLossContingencies_6d478d7e-9944-4ea6-9331-ceb7f87fe79e" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/SegmentsOfBusinessScheduleOfSegmentsOfBusinessDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#SegmentsOfBusinessScheduleOfSegmentsOfBusinessDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/SegmentsOfBusinessScheduleOfSegmentsOfBusinessDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentReportingInformationLineItems_6f6f2cb7-69a8-48ff-8fa7-650ce7d77903" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentReportingInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_6e22fcd2-803f-4022-b1b1-92ca57fdafb6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_6f6f2cb7-69a8-48ff-8fa7-650ce7d77903" xlink:to="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_6e22fcd2-803f-4022-b1b1-92ca57fdafb6" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PublicUtilityRegulatedOrUnregulatedStatusAxis_441214cc-e2d1-4fec-90fe-a32505b50068" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PublicUtilityRegulatedOrUnregulatedStatusAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_6e22fcd2-803f-4022-b1b1-92ca57fdafb6" xlink:to="loc_us-gaap_PublicUtilityRegulatedOrUnregulatedStatusAxis_441214cc-e2d1-4fec-90fe-a32505b50068" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatedAndUnregulatedOperationDomain_441214cc-e2d1-4fec-90fe-a32505b50068_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatedAndUnregulatedOperationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PublicUtilityRegulatedOrUnregulatedStatusAxis_441214cc-e2d1-4fec-90fe-a32505b50068" xlink:to="loc_us-gaap_RegulatedAndUnregulatedOperationDomain_441214cc-e2d1-4fec-90fe-a32505b50068_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatedAndUnregulatedOperationDomain_7b6549ae-9ab8-44ec-88dd-30e8d5cb54c0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatedAndUnregulatedOperationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PublicUtilityRegulatedOrUnregulatedStatusAxis_441214cc-e2d1-4fec-90fe-a32505b50068" xlink:to="loc_us-gaap_RegulatedAndUnregulatedOperationDomain_7b6549ae-9ab8-44ec-88dd-30e8d5cb54c0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatedOperationMember_78c06897-f50c-4742-bfa5-559f2a634648" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatedOperationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatedAndUnregulatedOperationDomain_7b6549ae-9ab8-44ec-88dd-30e8d5cb54c0" xlink:to="loc_us-gaap_RegulatedOperationMember_78c06897-f50c-4742-bfa5-559f2a634648" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_48e404b0-cafb-495e-a995-ead187ca3508" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_6e22fcd2-803f-4022-b1b1-92ca57fdafb6" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_48e404b0-cafb-495e-a995-ead187ca3508" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_48e404b0-cafb-495e-a995-ead187ca3508_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_48e404b0-cafb-495e-a995-ead187ca3508" xlink:to="loc_us-gaap_SegmentDomain_48e404b0-cafb-495e-a995-ead187ca3508_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_6172e968-7ac2-4689-b657-7d5438ea6173" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_48e404b0-cafb-495e-a995-ead187ca3508" xlink:to="loc_us-gaap_SegmentDomain_6172e968-7ac2-4689-b657-7d5438ea6173" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_ElectricMember_1ef5cbfa-bfc8-49f0-9e98-b04d3ed70d55" xlink:href="lnt-20240331.xsd#lnt_ElectricMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_6172e968-7ac2-4689-b657-7d5438ea6173" xlink:to="loc_lnt_ElectricMember_1ef5cbfa-bfc8-49f0-9e98-b04d3ed70d55" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_GasMember_d678928a-40b8-4b37-9eca-36b39c338333" xlink:href="lnt-20240331.xsd#lnt_GasMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_6172e968-7ac2-4689-b657-7d5438ea6173" xlink:to="loc_lnt_GasMember_d678928a-40b8-4b37-9eca-36b39c338333" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_OtherUtilityMember_764cba87-e35d-4310-90b6-2a133ba9cfb7" xlink:href="lnt-20240331.xsd#lnt_OtherUtilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_6172e968-7ac2-4689-b657-7d5438ea6173" xlink:to="loc_lnt_OtherUtilityMember_764cba87-e35d-4310-90b6-2a133ba9cfb7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllOtherSegmentsMember_3b58c94e-c6c8-4573-a7cd-11e442efa949" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AllOtherSegmentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_6172e968-7ac2-4689-b657-7d5438ea6173" xlink:to="loc_us-gaap_AllOtherSegmentsMember_3b58c94e-c6c8-4573-a7cd-11e442efa949" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_de1c4d95-aabb-4ab9-9b12-0e860402b482" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_6e22fcd2-803f-4022-b1b1-92ca57fdafb6" xlink:to="loc_dei_LegalEntityAxis_de1c4d95-aabb-4ab9-9b12-0e860402b482" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_de1c4d95-aabb-4ab9-9b12-0e860402b482_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_de1c4d95-aabb-4ab9-9b12-0e860402b482" xlink:to="loc_dei_EntityDomain_de1c4d95-aabb-4ab9-9b12-0e860402b482_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_339195b3-f4df-4532-ba34-0ed1b3232127" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_de1c4d95-aabb-4ab9-9b12-0e860402b482" xlink:to="loc_dei_EntityDomain_339195b3-f4df-4532-ba34-0ed1b3232127" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_382db94b-76e5-428d-b054-20de16d2c313" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_339195b3-f4df-4532-ba34-0ed1b3232127" xlink:to="loc_lnt_IplMember_382db94b-76e5-428d-b054-20de16d2c313" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_3271f42f-4237-4365-b9aa-b23679bd2dfa" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_339195b3-f4df-4532-ba34-0ed1b3232127" xlink:to="loc_lnt_WplMember_3271f42f-4237-4365-b9aa-b23679bd2dfa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatedAndUnregulatedOperatingRevenue_d058d2a3-d0de-4f84-9968-8d7184e12f0e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RegulatedAndUnregulatedOperatingRevenue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_6f6f2cb7-69a8-48ff-8fa7-650ce7d77903" xlink:to="loc_us-gaap_RegulatedAndUnregulatedOperatingRevenue_d058d2a3-d0de-4f84-9968-8d7184e12f0e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_e53d3c53-be76-4f07-97a1-b79a1cb88589" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_6f6f2cb7-69a8-48ff-8fa7-650ce7d77903" xlink:to="loc_us-gaap_OperatingIncomeLoss_e53d3c53-be76-4f07-97a1-b79a1cb88589" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic_2b3a77d9-28a5-4646-9a54-3121df256fd8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_6f6f2cb7-69a8-48ff-8fa7-650ce7d77903" xlink:to="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic_2b3a77d9-28a5-4646-9a54-3121df256fd8" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_ea3ac18f-8469-4615-a2e8-aed58bbcf545" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_6f6f2cb7-69a8-48ff-8fa7-650ce7d77903" xlink:to="loc_us-gaap_NetIncomeLoss_ea3ac18f-8469-4615-a2e8-aed58bbcf545" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/RelatedPartiesNarrativeDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#RelatedPartiesNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/RelatedPartiesNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionLineItems_618e9486-79ce-4969-8426-46c7c526e25d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyTransactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_2ccc800c-0694-4d1d-b4b7-7b237a4d11e3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_618e9486-79ce-4969-8426-46c7c526e25d" xlink:to="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_2ccc800c-0694-4d1d-b4b7-7b237a4d11e3" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_7e08d393-094c-48cc-a32f-b52506542672" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_2ccc800c-0694-4d1d-b4b7-7b237a4d11e3" xlink:to="loc_dei_LegalEntityAxis_7e08d393-094c-48cc-a32f-b52506542672" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_7e08d393-094c-48cc-a32f-b52506542672_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_7e08d393-094c-48cc-a32f-b52506542672" xlink:to="loc_dei_EntityDomain_7e08d393-094c-48cc-a32f-b52506542672_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_a2b9c2cf-8681-44ff-b8d8-721305884393" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_7e08d393-094c-48cc-a32f-b52506542672" xlink:to="loc_dei_EntityDomain_a2b9c2cf-8681-44ff-b8d8-721305884393" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_0920d145-28af-4424-8198-0ccd6ab8601b" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_a2b9c2cf-8681-44ff-b8d8-721305884393" xlink:to="loc_lnt_WplMember_0920d145-28af-4424-8198-0ccd6ab8601b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CounterpartyNameAxis_ee114c81-c0fb-4e93-98d3-8effaefbcd99" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_CounterpartyNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_2ccc800c-0694-4d1d-b4b7-7b237a4d11e3" xlink:to="loc_srt_CounterpartyNameAxis_ee114c81-c0fb-4e93-98d3-8effaefbcd99" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_ee114c81-c0fb-4e93-98d3-8effaefbcd99_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CounterpartyNameAxis_ee114c81-c0fb-4e93-98d3-8effaefbcd99" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_ee114c81-c0fb-4e93-98d3-8effaefbcd99_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_264a07b8-2805-4809-93bd-bba77aab4aca" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CounterpartyNameAxis_ee114c81-c0fb-4e93-98d3-8effaefbcd99" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_264a07b8-2805-4809-93bd-bba77aab4aca" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_AmericanTransmissionCompanyLlcAtcMember_d058fb11-89a6-4e31-b676-7b5cb4dde9fb" xlink:href="lnt-20240331.xsd#lnt_AmericanTransmissionCompanyLlcAtcMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_264a07b8-2805-4809-93bd-bba77aab4aca" xlink:to="loc_lnt_AmericanTransmissionCompanyLlcAtcMember_d058fb11-89a6-4e31-b676-7b5cb4dde9fb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionAxis_75d689d5-ed43-4af3-bf43-47cf58213809" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyTransactionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_2ccc800c-0694-4d1d-b4b7-7b237a4d11e3" xlink:to="loc_us-gaap_RelatedPartyTransactionAxis_75d689d5-ed43-4af3-bf43-47cf58213809" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionDomain_75d689d5-ed43-4af3-bf43-47cf58213809_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RelatedPartyTransactionAxis_75d689d5-ed43-4af3-bf43-47cf58213809" xlink:to="loc_us-gaap_RelatedPartyTransactionDomain_75d689d5-ed43-4af3-bf43-47cf58213809_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionDomain_1c15b20b-7565-41cd-81fb-296ec17bf45f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RelatedPartyTransactionAxis_75d689d5-ed43-4af3-bf43-47cf58213809" xlink:to="loc_us-gaap_RelatedPartyTransactionDomain_1c15b20b-7565-41cd-81fb-296ec17bf45f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplOwedAtcMember_3e01a04d-da9d-44fb-8300-c3a796021475" xlink:href="lnt-20240331.xsd#lnt_WplOwedAtcMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_1c15b20b-7565-41cd-81fb-296ec17bf45f" xlink:to="loc_lnt_WplOwedAtcMember_3e01a04d-da9d-44fb-8300-c3a796021475" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_8c4c4fbc-d1ac-496a-8464-5d6698270bb2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyTransactionsByRelatedPartyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_2ccc800c-0694-4d1d-b4b7-7b237a4d11e3" xlink:to="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_8c4c4fbc-d1ac-496a-8464-5d6698270bb2" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_8c4c4fbc-d1ac-496a-8464-5d6698270bb2_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_8c4c4fbc-d1ac-496a-8464-5d6698270bb2" xlink:to="loc_us-gaap_RelatedPartyDomain_8c4c4fbc-d1ac-496a-8464-5d6698270bb2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_5337e60d-282b-4c52-b21b-6f45bcb96473" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_8c4c4fbc-d1ac-496a-8464-5d6698270bb2" xlink:to="loc_us-gaap_RelatedPartyDomain_5337e60d-282b-4c52-b21b-6f45bcb96473" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyMember_ff75a28a-ec77-4e0e-85ec-ff25a55cfcff" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyDomain_5337e60d-282b-4c52-b21b-6f45bcb96473" xlink:to="loc_us-gaap_RelatedPartyMember_ff75a28a-ec77-4e0e-85ec-ff25a55cfcff" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilities_ab3109aa-9a21-4ee5-9a77-9b7f0d529aad" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_618e9486-79ce-4969-8426-46c7c526e25d" xlink:to="loc_us-gaap_OtherLiabilities_ab3109aa-9a21-4ee5-9a77-9b7f0d529aad" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/RelatedPartiesServiceAgreementsDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#RelatedPartiesServiceAgreementsDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/RelatedPartiesServiceAgreementsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionLineItems_4be0914d-864a-421a-8c13-70c1c943c591" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyTransactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_60b8ff12-beb9-4210-93df-9d8185ac8c12" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_4be0914d-864a-421a-8c13-70c1c943c591" xlink:to="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_60b8ff12-beb9-4210-93df-9d8185ac8c12" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionAxis_414e5dad-50d2-47ed-9521-e30b5629066c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyTransactionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_60b8ff12-beb9-4210-93df-9d8185ac8c12" xlink:to="loc_us-gaap_RelatedPartyTransactionAxis_414e5dad-50d2-47ed-9521-e30b5629066c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionDomain_414e5dad-50d2-47ed-9521-e30b5629066c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RelatedPartyTransactionAxis_414e5dad-50d2-47ed-9521-e30b5629066c" xlink:to="loc_us-gaap_RelatedPartyTransactionDomain_414e5dad-50d2-47ed-9521-e30b5629066c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionDomain_d9010281-c82e-4204-97be-bb0b26468afd" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RelatedPartyTransactionAxis_414e5dad-50d2-47ed-9521-e30b5629066c" xlink:to="loc_us-gaap_RelatedPartyTransactionDomain_d9010281-c82e-4204-97be-bb0b26468afd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_AdministrativeandGeneralServicesBillingsMember_f5b4d751-5f1b-4e63-aa61-1bd13f4fa15e" xlink:href="lnt-20240331.xsd#lnt_AdministrativeandGeneralServicesBillingsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_d9010281-c82e-4204-97be-bb0b26468afd" xlink:to="loc_lnt_AdministrativeandGeneralServicesBillingsMember_f5b4d751-5f1b-4e63-aa61-1bd13f4fa15e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_TransmissionSalesCreditedMember_17633141-1734-47b7-9119-55e072aa31ef" xlink:href="lnt-20240331.xsd#lnt_TransmissionSalesCreditedMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_d9010281-c82e-4204-97be-bb0b26468afd" xlink:to="loc_lnt_TransmissionSalesCreditedMember_17633141-1734-47b7-9119-55e072aa31ef" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_TransmissionPurchasesBilledMember_027b5c0b-8ed6-4666-82c9-f012a364ba01" xlink:href="lnt-20240331.xsd#lnt_TransmissionPurchasesBilledMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_d9010281-c82e-4204-97be-bb0b26468afd" xlink:to="loc_lnt_TransmissionPurchasesBilledMember_027b5c0b-8ed6-4666-82c9-f012a364ba01" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CounterpartyNameAxis_36a0a5de-923e-4a8d-9363-606873503b28" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_CounterpartyNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_60b8ff12-beb9-4210-93df-9d8185ac8c12" xlink:to="loc_srt_CounterpartyNameAxis_36a0a5de-923e-4a8d-9363-606873503b28" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_36a0a5de-923e-4a8d-9363-606873503b28_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CounterpartyNameAxis_36a0a5de-923e-4a8d-9363-606873503b28" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_36a0a5de-923e-4a8d-9363-606873503b28_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_58761454-22c4-4858-ba55-886fa37a7539" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CounterpartyNameAxis_36a0a5de-923e-4a8d-9363-606873503b28" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_58761454-22c4-4858-ba55-886fa37a7539" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_CorporateServicesMember_ffcd7391-aa2d-4c2b-bb20-db5f76b93660" xlink:href="lnt-20240331.xsd#lnt_CorporateServicesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_58761454-22c4-4858-ba55-886fa37a7539" xlink:to="loc_lnt_CorporateServicesMember_ffcd7391-aa2d-4c2b-bb20-db5f76b93660" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_7d8edaf5-ea77-4cdf-8d4a-378dc1098cea" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyTransactionsByRelatedPartyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_60b8ff12-beb9-4210-93df-9d8185ac8c12" xlink:to="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_7d8edaf5-ea77-4cdf-8d4a-378dc1098cea" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_7d8edaf5-ea77-4cdf-8d4a-378dc1098cea_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_7d8edaf5-ea77-4cdf-8d4a-378dc1098cea" xlink:to="loc_us-gaap_RelatedPartyDomain_7d8edaf5-ea77-4cdf-8d4a-378dc1098cea_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_98e0c2a8-d9aa-4c2c-a49e-7cdd56e66c96" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_7d8edaf5-ea77-4cdf-8d4a-378dc1098cea" xlink:to="loc_us-gaap_RelatedPartyDomain_98e0c2a8-d9aa-4c2c-a49e-7cdd56e66c96" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsidiaryOfCommonParentMember_098cc321-1b61-40c7-9cb9-cd69affdbce0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsidiaryOfCommonParentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyDomain_98e0c2a8-d9aa-4c2c-a49e-7cdd56e66c96" xlink:to="loc_us-gaap_SubsidiaryOfCommonParentMember_098cc321-1b61-40c7-9cb9-cd69affdbce0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_763aec4a-808d-4e25-9d88-72df7b6a47cf" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_60b8ff12-beb9-4210-93df-9d8185ac8c12" xlink:to="loc_dei_LegalEntityAxis_763aec4a-808d-4e25-9d88-72df7b6a47cf" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_763aec4a-808d-4e25-9d88-72df7b6a47cf_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_763aec4a-808d-4e25-9d88-72df7b6a47cf" xlink:to="loc_dei_EntityDomain_763aec4a-808d-4e25-9d88-72df7b6a47cf_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_4c36f745-3607-474b-bc75-4d95cc29233e" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_763aec4a-808d-4e25-9d88-72df7b6a47cf" xlink:to="loc_dei_EntityDomain_4c36f745-3607-474b-bc75-4d95cc29233e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_82956a62-43f0-4ca3-8206-1220fb4663d3" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_4c36f745-3607-474b-bc75-4d95cc29233e" xlink:to="loc_lnt_IplMember_82956a62-43f0-4ca3-8206-1220fb4663d3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_46858423-f3d1-4528-a166-6b56642afc7f" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_4c36f745-3607-474b-bc75-4d95cc29233e" xlink:to="loc_lnt_WplMember_46858423-f3d1-4528-a166-6b56642afc7f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionAmountsOfTransaction_515e3a5d-78f7-4e15-8078-c62fefff7113" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyTransactionAmountsOfTransaction"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_4be0914d-864a-421a-8c13-70c1c943c591" xlink:to="loc_us-gaap_RelatedPartyTransactionAmountsOfTransaction_515e3a5d-78f7-4e15-8078-c62fefff7113" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/RelatedPartiesNetIntercompanyPayablesDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#RelatedPartiesNetIntercompanyPayablesDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/RelatedPartiesNetIntercompanyPayablesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionLineItems_34ef687e-f617-415e-9c8e-53233ea5a33b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyTransactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_2cc58336-e5cb-4894-a244-78680fe1570f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_34ef687e-f617-415e-9c8e-53233ea5a33b" xlink:to="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_2cc58336-e5cb-4894-a244-78680fe1570f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_9dab1748-4a9e-44b5-9288-3a6dbdf79c80" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyTransactionsByRelatedPartyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_2cc58336-e5cb-4894-a244-78680fe1570f" xlink:to="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_9dab1748-4a9e-44b5-9288-3a6dbdf79c80" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_9dab1748-4a9e-44b5-9288-3a6dbdf79c80_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_9dab1748-4a9e-44b5-9288-3a6dbdf79c80" xlink:to="loc_us-gaap_RelatedPartyDomain_9dab1748-4a9e-44b5-9288-3a6dbdf79c80_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_1536b325-473f-4a79-b33b-ae6210c19b26" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_9dab1748-4a9e-44b5-9288-3a6dbdf79c80" xlink:to="loc_us-gaap_RelatedPartyDomain_1536b325-473f-4a79-b33b-ae6210c19b26" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsidiaryOfCommonParentMember_f5dee5d9-f6b1-4205-adab-27ef872c1d53" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SubsidiaryOfCommonParentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyDomain_1536b325-473f-4a79-b33b-ae6210c19b26" xlink:to="loc_us-gaap_SubsidiaryOfCommonParentMember_f5dee5d9-f6b1-4205-adab-27ef872c1d53" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyMember_660cb069-3e52-488b-a137-6e3663381562" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyDomain_1536b325-473f-4a79-b33b-ae6210c19b26" xlink:to="loc_us-gaap_RelatedPartyMember_660cb069-3e52-488b-a137-6e3663381562" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CounterpartyNameAxis_ec26408f-7476-4817-b576-d79f9fa00944" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_CounterpartyNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_2cc58336-e5cb-4894-a244-78680fe1570f" xlink:to="loc_srt_CounterpartyNameAxis_ec26408f-7476-4817-b576-d79f9fa00944" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_ec26408f-7476-4817-b576-d79f9fa00944_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CounterpartyNameAxis_ec26408f-7476-4817-b576-d79f9fa00944" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_ec26408f-7476-4817-b576-d79f9fa00944_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_132841b3-c97f-4297-96b4-77e71723e582" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CounterpartyNameAxis_ec26408f-7476-4817-b576-d79f9fa00944" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_132841b3-c97f-4297-96b4-77e71723e582" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_CorporateServicesMember_24356609-72ae-4bee-ab0f-dec0b88ed229" xlink:href="lnt-20240331.xsd#lnt_CorporateServicesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_132841b3-c97f-4297-96b4-77e71723e582" xlink:to="loc_lnt_CorporateServicesMember_24356609-72ae-4bee-ab0f-dec0b88ed229" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_4da644b0-6991-4521-ae9d-ca2e9e000346" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_2cc58336-e5cb-4894-a244-78680fe1570f" xlink:to="loc_dei_LegalEntityAxis_4da644b0-6991-4521-ae9d-ca2e9e000346" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_4da644b0-6991-4521-ae9d-ca2e9e000346_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_4da644b0-6991-4521-ae9d-ca2e9e000346" xlink:to="loc_dei_EntityDomain_4da644b0-6991-4521-ae9d-ca2e9e000346_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_3beaf4dd-2685-4684-b3ac-8be6268a0a4e" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_4da644b0-6991-4521-ae9d-ca2e9e000346" xlink:to="loc_dei_EntityDomain_3beaf4dd-2685-4684-b3ac-8be6268a0a4e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_IplMember_505349a0-c7e7-41b0-80bb-816de92b2e88" xlink:href="lnt-20240331.xsd#lnt_IplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_3beaf4dd-2685-4684-b3ac-8be6268a0a4e" xlink:to="loc_lnt_IplMember_505349a0-c7e7-41b0-80bb-816de92b2e88" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_c21c34df-9659-4c84-bf2c-c442ec2a8273" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_3beaf4dd-2685-4684-b3ac-8be6268a0a4e" xlink:to="loc_lnt_WplMember_c21c34df-9659-4c84-bf2c-c442ec2a8273" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilities_f6be8892-cf8c-47f8-b2c2-58599961016b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_34ef687e-f617-415e-9c8e-53233ea5a33b" xlink:to="loc_us-gaap_OtherLiabilities_f6be8892-cf8c-47f8-b2c2-58599961016b" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.alliantenergy.com/role/RelatedPartiesAmountsBilledBetweenPartiesDetails" xlink:type="simple" xlink:href="lnt-20240331.xsd#RelatedPartiesAmountsBilledBetweenPartiesDetails"/>
  <link:definitionLink xlink:role="http://www.alliantenergy.com/role/RelatedPartiesAmountsBilledBetweenPartiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionLineItems_1475bbd5-0335-40c6-8a7d-5c3791e3803a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyTransactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_1bea9ac3-1530-48b3-af62-4f54aca7a611" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_1475bbd5-0335-40c6-8a7d-5c3791e3803a" xlink:to="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_1bea9ac3-1530-48b3-af62-4f54aca7a611" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_72425448-e6c1-478e-aa38-665689b541d8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyTransactionsByRelatedPartyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_1bea9ac3-1530-48b3-af62-4f54aca7a611" xlink:to="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_72425448-e6c1-478e-aa38-665689b541d8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_72425448-e6c1-478e-aa38-665689b541d8_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_72425448-e6c1-478e-aa38-665689b541d8" xlink:to="loc_us-gaap_RelatedPartyDomain_72425448-e6c1-478e-aa38-665689b541d8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_c8be261b-7ed9-49d4-a76c-ad63c8f6adad" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_72425448-e6c1-478e-aa38-665689b541d8" xlink:to="loc_us-gaap_RelatedPartyDomain_c8be261b-7ed9-49d4-a76c-ad63c8f6adad" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_AtcBillingsToWplMember_f045fad5-6c82-4329-b7cc-be612fe84af8" xlink:href="lnt-20240331.xsd#lnt_AtcBillingsToWplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyDomain_c8be261b-7ed9-49d4-a76c-ad63c8f6adad" xlink:to="loc_lnt_AtcBillingsToWplMember_f045fad5-6c82-4329-b7cc-be612fe84af8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplBillingsToAtcMember_ccb36225-7c56-48b6-b578-f0e100cd9acd" xlink:href="lnt-20240331.xsd#lnt_WplBillingsToAtcMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyDomain_c8be261b-7ed9-49d4-a76c-ad63c8f6adad" xlink:to="loc_lnt_WplBillingsToAtcMember_ccb36225-7c56-48b6-b578-f0e100cd9acd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CounterpartyNameAxis_ca81e563-98f6-46f6-be7f-540bc00e5f15" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_CounterpartyNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_1bea9ac3-1530-48b3-af62-4f54aca7a611" xlink:to="loc_srt_CounterpartyNameAxis_ca81e563-98f6-46f6-be7f-540bc00e5f15" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_ca81e563-98f6-46f6-be7f-540bc00e5f15_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CounterpartyNameAxis_ca81e563-98f6-46f6-be7f-540bc00e5f15" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_ca81e563-98f6-46f6-be7f-540bc00e5f15_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_e0d40cdc-47a6-4eef-a26d-4d1ac2aaee57" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CounterpartyNameAxis_ca81e563-98f6-46f6-be7f-540bc00e5f15" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_e0d40cdc-47a6-4eef-a26d-4d1ac2aaee57" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_AmericanTransmissionCompanyLlcAtcMember_f62dea21-2a25-4aa1-ad08-52e4a52da4b0" xlink:href="lnt-20240331.xsd#lnt_AmericanTransmissionCompanyLlcAtcMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_e0d40cdc-47a6-4eef-a26d-4d1ac2aaee57" xlink:to="loc_lnt_AmericanTransmissionCompanyLlcAtcMember_f62dea21-2a25-4aa1-ad08-52e4a52da4b0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_bad9c0bb-c451-4779-b0b4-346ec95e9b3d" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_1bea9ac3-1530-48b3-af62-4f54aca7a611" xlink:to="loc_dei_LegalEntityAxis_bad9c0bb-c451-4779-b0b4-346ec95e9b3d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_bad9c0bb-c451-4779-b0b4-346ec95e9b3d_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_bad9c0bb-c451-4779-b0b4-346ec95e9b3d" xlink:to="loc_dei_EntityDomain_bad9c0bb-c451-4779-b0b4-346ec95e9b3d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_82becea6-eb7a-437c-9543-9fa43e26e890" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_bad9c0bb-c451-4779-b0b4-346ec95e9b3d" xlink:to="loc_dei_EntityDomain_82becea6-eb7a-437c-9543-9fa43e26e890" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_WplMember_4af50379-85cf-43fa-b271-00d9661e3eef" xlink:href="lnt-20240331.xsd#lnt_WplMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_82becea6-eb7a-437c-9543-9fa43e26e890" xlink:to="loc_lnt_WplMember_4af50379-85cf-43fa-b271-00d9661e3eef" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionAmountsOfTransaction_266e6d90-fd6e-419a-8c9a-f12a308724b0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RelatedPartyTransactionAmountsOfTransaction"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_1475bbd5-0335-40c6-8a7d-5c3791e3803a" xlink:to="loc_us-gaap_RelatedPartyTransactionAmountsOfTransaction_266e6d90-fd6e-419a-8c9a-f12a308724b0" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://xbrl.sec.gov/ecd/AwardTypeOnly" xlink:type="simple" xlink:href="https://xbrl.sec.gov/ecd/2023/ecd-2023.xsd#AwardTypeOnly"/>
  <link:definitionLink xlink:role="http://xbrl.sec.gov/ecd/AwardTypeOnly" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PerformanceSharesMember" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PerformanceSharesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" xlink:to="loc_us-gaap_PerformanceSharesMember" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_PerformanceRestrictedStockUnitMember_75c79235-27b9-458b-a836-ca97ac42b88a" xlink:href="lnt-20240331.xsd#lnt_PerformanceRestrictedStockUnitMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" xlink:to="loc_lnt_PerformanceRestrictedStockUnitMember_75c79235-27b9-458b-a836-ca97ac42b88a" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_lnt_OmnibusIncentivePlanMember_b7249033-0084-4e23-8e64-704448f1e893" xlink:href="lnt-20240331.xsd#lnt_OmnibusIncentivePlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" xlink:to="loc_lnt_OmnibusIncentivePlanMember_b7249033-0084-4e23-8e64-704448f1e893" xlink:type="arc" order="7"/>
  </link:definitionLink>
</link:linkbase>
