<?xml version="1.0" encoding="US-ASCII"?>
<!--XBRL Document Created with Wdesk from Workiva, formerly WebFilings-->
<!-- -->
<linkbase xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd" xmlns="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <roleRef roleURI="http://www.hologic.com/role/BasisOfPresentation" xlink:href="holx-20150328.xsd#BasisOfPresentation" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/BorrowingsAndCreditArrangements" xlink:href="holx-20150328.xsd#BorrowingsAndCreditArrangements" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/BorrowingsAndCreditArrangementsAdditionalInformationDetail" xlink:href="holx-20150328.xsd#BorrowingsAndCreditArrangementsAdditionalInformationDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/BorrowingsAndCreditArrangementsCompanysBorrowingsDetail" xlink:href="holx-20150328.xsd#BorrowingsAndCreditArrangementsCompanysBorrowingsDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/BorrowingsAndCreditArrangementsInterestExpenseUnderConvertibleNotesDetail" xlink:href="holx-20150328.xsd#BorrowingsAndCreditArrangementsInterestExpenseUnderConvertibleNotesDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/BorrowingsAndCreditArrangementsTables" xlink:href="holx-20150328.xsd#BorrowingsAndCreditArrangementsTables" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/BusinessSegmentsAndGeographicInformation" xlink:href="holx-20150328.xsd#BusinessSegmentsAndGeographicInformation" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/BusinessSegmentsAndGeographicInformationAdditionalInformationDetail" xlink:href="holx-20150328.xsd#BusinessSegmentsAndGeographicInformationAdditionalInformationDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/BusinessSegmentsAndGeographicInformationRevenuesByGeographyDetail" xlink:href="holx-20150328.xsd#BusinessSegmentsAndGeographicInformationRevenuesByGeographyDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/BusinessSegmentsAndGeographicInformationSegmentInformationDetail" xlink:href="holx-20150328.xsd#BusinessSegmentsAndGeographicInformationSegmentInformationDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/BusinessSegmentsAndGeographicInformationTables" xlink:href="holx-20150328.xsd#BusinessSegmentsAndGeographicInformationTables" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/CommitmentsAndContingencies" xlink:href="holx-20150328.xsd#CommitmentsAndContingencies" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/CommitmentsAndContingenciesAdditionalInformationDetail" xlink:href="holx-20150328.xsd#CommitmentsAndContingenciesAdditionalInformationDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/ConsolidatedBalanceSheets" xlink:href="holx-20150328.xsd#ConsolidatedBalanceSheets" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/ConsolidatedBalanceSheetsParenthetical" xlink:href="holx-20150328.xsd#ConsolidatedBalanceSheetsParenthetical" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/ConsolidatedStatementsOfCashFlows" xlink:href="holx-20150328.xsd#ConsolidatedStatementsOfCashFlows" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/ConsolidatedStatementsOfComprehensiveIncomeLoss" xlink:href="holx-20150328.xsd#ConsolidatedStatementsOfComprehensiveIncomeLoss" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/ConsolidatedStatementsOfComprehensiveIncomeLossParenthetical" xlink:href="holx-20150328.xsd#ConsolidatedStatementsOfComprehensiveIncomeLossParenthetical" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/ConsolidatedStatementsOfOperations" xlink:href="holx-20150328.xsd#ConsolidatedStatementsOfOperations" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/DerivativesAdditionalInformationDetails" xlink:href="holx-20150328.xsd#DerivativesAdditionalInformationDetails" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/DerivativesNotes" xlink:href="holx-20150328.xsd#DerivativesNotes" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/DerivativesScheduleOfDerivativeInstrumentsOnConsolidatedBalanceSheetsAndUnrealizedLossRecognizedInAociDetails" xlink:href="holx-20150328.xsd#DerivativesScheduleOfDerivativeInstrumentsOnConsolidatedBalanceSheetsAndUnrealizedLossRecognizedInAociDetails" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/DerivativesTables" xlink:href="holx-20150328.xsd#DerivativesTables" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/DocumentAndEntityInformation" xlink:href="holx-20150328.xsd#DocumentAndEntityInformation" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/FairValueMeasurements" xlink:href="holx-20150328.xsd#FairValueMeasurements" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/FairValueMeasurementsAdditionalInformationDetail" xlink:href="holx-20150328.xsd#FairValueMeasurementsAdditionalInformationDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/FairValueMeasurementsEstimatedFairValuesOfConvertibleNotesDetail" xlink:href="holx-20150328.xsd#FairValueMeasurementsEstimatedFairValuesOfConvertibleNotesDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/FairValueMeasurementsFairValueAssetsAndLiabilitiesMeasuredOnRecurringBasisDetail" xlink:href="holx-20150328.xsd#FairValueMeasurementsFairValueAssetsAndLiabilitiesMeasuredOnRecurringBasisDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/FairValueMeasurementsTables" xlink:href="holx-20150328.xsd#FairValueMeasurementsTables" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/IncomeTaxes" xlink:href="holx-20150328.xsd#IncomeTaxes" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/IncomeTaxesAdditionalInformationDetail" xlink:href="holx-20150328.xsd#IncomeTaxesAdditionalInformationDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/IntangibleAssets" xlink:href="holx-20150328.xsd#IntangibleAssets" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/IntangibleAssetsAdditionalInformationDetail" xlink:href="holx-20150328.xsd#IntangibleAssetsAdditionalInformationDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/IntangibleAssetsScheduleOfEstimatedAmortizationExpenseDetail" xlink:href="holx-20150328.xsd#IntangibleAssetsScheduleOfEstimatedAmortizationExpenseDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/IntangibleAssetsScheduleOfIntangibleAssetsDetail" xlink:href="holx-20150328.xsd#IntangibleAssetsScheduleOfIntangibleAssetsDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/IntangibleAssetsTables" xlink:href="holx-20150328.xsd#IntangibleAssetsTables" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/MarketableSecurities" xlink:href="holx-20150328.xsd#MarketableSecurities" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/MarketableSecuritiesReconcilesCostBasisToFairMarketValueOfCompanysEquitySecurityDetail" xlink:href="holx-20150328.xsd#MarketableSecuritiesReconcilesCostBasisToFairMarketValueOfCompanysEquitySecurityDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/MarketableSecuritiesTables" xlink:href="holx-20150328.xsd#MarketableSecuritiesTables" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/NetIncomeLossPerShare" xlink:href="holx-20150328.xsd#NetIncomeLossPerShare" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/NetIncomeLossPerShareReconciliationOfBasicAndDilutedShareAmountsDetail" xlink:href="holx-20150328.xsd#NetIncomeLossPerShareReconciliationOfBasicAndDilutedShareAmountsDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/NetIncomeLossPerShareTables" xlink:href="holx-20150328.xsd#NetIncomeLossPerShareTables" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/NewAccountingPronouncements" xlink:href="holx-20150328.xsd#NewAccountingPronouncements" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/NewAccountingPronouncementsPolicies" xlink:href="holx-20150328.xsd#NewAccountingPronouncementsPolicies" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/OtherBalanceSheetInformation" xlink:href="holx-20150328.xsd#OtherBalanceSheetInformation" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/OtherBalanceSheetInformationOtherBalanceSheetInformationOfInventoriesDetail" xlink:href="holx-20150328.xsd#OtherBalanceSheetInformationOtherBalanceSheetInformationOfInventoriesDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/OtherBalanceSheetInformationOtherBalanceSheetInformationOfPropertyPlantAndEquipmentDetail" xlink:href="holx-20150328.xsd#OtherBalanceSheetInformationOtherBalanceSheetInformationOfPropertyPlantAndEquipmentDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/OtherBalanceSheetInformationTables" xlink:href="holx-20150328.xsd#OtherBalanceSheetInformationTables" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/ProductWarranties" xlink:href="holx-20150328.xsd#ProductWarranties" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/ProductWarrantiesProductWarrantyDetail" xlink:href="holx-20150328.xsd#ProductWarrantiesProductWarrantyDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/ProductWarrantiesTables" xlink:href="holx-20150328.xsd#ProductWarrantiesTables" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/RestructuringAndDivestitureCharges" xlink:href="holx-20150328.xsd#RestructuringAndDivestitureCharges" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/RestructuringAndDivestitureChargesAdditionalInformationDetail" xlink:href="holx-20150328.xsd#RestructuringAndDivestitureChargesAdditionalInformationDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/RestructuringAndDivestitureChargesChargesTakenRelatedToAccruedRestructuringActionsDetail" xlink:href="holx-20150328.xsd#RestructuringAndDivestitureChargesChargesTakenRelatedToAccruedRestructuringActionsDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/RestructuringAndDivestitureChargesChargesTakenRelatedToRestructuringActionsDetail" xlink:href="holx-20150328.xsd#RestructuringAndDivestitureChargesChargesTakenRelatedToRestructuringActionsDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/RestructuringAndDivestitureChargesTables" xlink:href="holx-20150328.xsd#RestructuringAndDivestitureChargesTables" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/StockBasedCompensation" xlink:href="holx-20150328.xsd#StockBasedCompensation" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/StockBasedCompensationAdditionalInformationDetail" xlink:href="holx-20150328.xsd#StockBasedCompensationAdditionalInformationDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/StockBasedCompensationStockBasedCompensationExpenseInConsolidatedStatementsOfOperationsDetail" xlink:href="holx-20150328.xsd#StockBasedCompensationStockBasedCompensationExpenseInConsolidatedStatementsOfOperationsDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/StockBasedCompensationTables" xlink:href="holx-20150328.xsd#StockBasedCompensationTables" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/StockBasedCompensationWeightedAverageAssumptionsUtilizedToValueStockOptionsDetail" xlink:href="holx-20150328.xsd#StockBasedCompensationWeightedAverageAssumptionsUtilizedToValueStockOptionsDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/SupplementalGuarantorCondensedConsolidatingFinancials" xlink:href="holx-20150328.xsd#SupplementalGuarantorCondensedConsolidatingFinancials" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/SupplementalGuarantorCondensedConsolidatingFinancialsAdditionalInformationDetail" xlink:href="holx-20150328.xsd#SupplementalGuarantorCondensedConsolidatingFinancialsAdditionalInformationDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/SupplementalGuarantorCondensedConsolidatingFinancialsScheduleOfConsolidatingStatementOfCashFlowsDetail" xlink:href="holx-20150328.xsd#SupplementalGuarantorCondensedConsolidatingFinancialsScheduleOfConsolidatingStatementOfCashFlowsDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/SupplementalGuarantorCondensedConsolidatingFinancialsScheduleOfSupplementalCondensedConsolidatingBalanceSheetDetail" xlink:href="holx-20150328.xsd#SupplementalGuarantorCondensedConsolidatingFinancialsScheduleOfSupplementalCondensedConsolidatingBalanceSheetDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/SupplementalGuarantorCondensedConsolidatingFinancialsScheduleOfSupplementalCondensedConsolidatingStatementOfOperationsDetail" xlink:href="holx-20150328.xsd#SupplementalGuarantorCondensedConsolidatingFinancialsScheduleOfSupplementalCondensedConsolidatingStatementOfOperationsDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/SupplementalGuarantorCondensedConsolidatingFinancialsScheduleOfSupplementalCondensedConsolidatingStatementsOfComprehensiveLossIncomeDetail" xlink:href="holx-20150328.xsd#SupplementalGuarantorCondensedConsolidatingFinancialsScheduleOfSupplementalCondensedConsolidatingStatementsOfComprehensiveLossIncomeDetail" xlink:type="simple" />
  <roleRef roleURI="http://www.hologic.com/role/SupplementalGuarantorCondensedConsolidatingFinancialsTables" xlink:href="holx-20150328.xsd#SupplementalGuarantorCondensedConsolidatingFinancialsTables" xlink:type="simple" />
  <calculationLink xlink:role="http://www.hologic.com/role/BasisOfPresentation" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/BorrowingsAndCreditArrangements" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/BorrowingsAndCreditArrangementsAdditionalInformationDetail" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/BorrowingsAndCreditArrangementsCompanysBorrowingsDetail" xlink:type="extended">
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_LongTermDebt" xlink:label="loc_us-gaap_LongTermDebt_8aeb26df-09df-f3e3-fe25-1f144575031f" xlink:type="locator" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_LongTermDebtNoncurrent" xlink:label="loc_us-gaap_LongTermDebtNoncurrent_89b83a57-ffbc-fc48-170d-f8b25d163e54" xlink:type="locator" />
    <calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LongTermDebt_8aeb26df-09df-f3e3-fe25-1f144575031f" xlink:to="loc_us-gaap_LongTermDebtNoncurrent_89b83a57-ffbc-fc48-170d-f8b25d163e54" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_LongTermDebtCurrent" xlink:label="loc_us-gaap_LongTermDebtCurrent_0601d697-1537-c3cb-caaf-11141ac92b39" xlink:type="locator" />
    <calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LongTermDebt_8aeb26df-09df-f3e3-fe25-1f144575031f" xlink:to="loc_us-gaap_LongTermDebtCurrent_0601d697-1537-c3cb-caaf-11141ac92b39" xlink:type="arc" />
  </calculationLink>
  <calculationLink xlink:role="http://www.hologic.com/role/BorrowingsAndCreditArrangementsInterestExpenseUnderConvertibleNotesDetail" xlink:type="extended">
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_InterestExpenseDebt" xlink:label="loc_us-gaap_InterestExpenseDebt_ed80f6f7-9d34-9c9b-5b6e-0410496d5d18" xlink:type="locator" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_AmortizationOfFinancingCostsAndDiscounts" xlink:label="loc_us-gaap_AmortizationOfFinancingCostsAndDiscounts_cad4dd20-f895-7d13-fef5-2f2bffdf14ac" xlink:type="locator" />
    <calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InterestExpenseDebt_ed80f6f7-9d34-9c9b-5b6e-0410496d5d18" xlink:to="loc_us-gaap_AmortizationOfFinancingCostsAndDiscounts_cad4dd20-f895-7d13-fef5-2f2bffdf14ac" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_AmortizationOfDebtDiscountPremium" xlink:label="loc_us-gaap_AmortizationOfDebtDiscountPremium_75fd7616-c8e6-4ab2-69c1-8f65632e3706" xlink:type="locator" />
    <calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AmortizationOfFinancingCostsAndDiscounts_cad4dd20-f895-7d13-fef5-2f2bffdf14ac" xlink:to="loc_us-gaap_AmortizationOfDebtDiscountPremium_75fd7616-c8e6-4ab2-69c1-8f65632e3706" xlink:type="arc" />
    <loc xlink:href="holx-20150328.xsd#holx_AmortizationOfDeferredFinancingCosts" xlink:label="loc_holx_AmortizationOfDeferredFinancingCosts_d2b03161-78e6-5a62-6fcb-9b2218baf3fc" xlink:type="locator" />
    <calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AmortizationOfFinancingCostsAndDiscounts_cad4dd20-f895-7d13-fef5-2f2bffdf14ac" xlink:to="loc_holx_AmortizationOfDeferredFinancingCosts_d2b03161-78e6-5a62-6fcb-9b2218baf3fc" xlink:type="arc" />
    <loc xlink:href="holx-20150328.xsd#holx_BasePrincipalAmountForAccretionValue" xlink:label="loc_holx_BasePrincipalAmountForAccretionValue_ff016207-879d-e918-4617-9b2219dc5a25" xlink:type="locator" />
    <calculationArc order="3" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AmortizationOfFinancingCostsAndDiscounts_cad4dd20-f895-7d13-fef5-2f2bffdf14ac" xlink:to="loc_holx_BasePrincipalAmountForAccretionValue_ff016207-879d-e918-4617-9b2219dc5a25" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_InterestExpenseDebtExcludingAmortization" xlink:label="loc_us-gaap_InterestExpenseDebtExcludingAmortization_320aec30-7d50-87cf-1187-d900ee4f2bda" xlink:type="locator" />
    <calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InterestExpenseDebt_ed80f6f7-9d34-9c9b-5b6e-0410496d5d18" xlink:to="loc_us-gaap_InterestExpenseDebtExcludingAmortization_320aec30-7d50-87cf-1187-d900ee4f2bda" xlink:type="arc" />
  </calculationLink>
  <calculationLink xlink:role="http://www.hologic.com/role/BorrowingsAndCreditArrangementsTables" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/BusinessSegmentsAndGeographicInformation" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/BusinessSegmentsAndGeographicInformationAdditionalInformationDetail" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/BusinessSegmentsAndGeographicInformationRevenuesByGeographyDetail" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/BusinessSegmentsAndGeographicInformationSegmentInformationDetail" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/BusinessSegmentsAndGeographicInformationTables" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/CommitmentsAndContingencies" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/CommitmentsAndContingenciesAdditionalInformationDetail" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/ConsolidatedBalanceSheets" xlink:type="extended">
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_LiabilitiesAndStockholdersEquity" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquity_ae9fd5e7-8741-dfbd-241e-1460ec6c786e" xlink:type="locator" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_LiabilitiesCurrent" xlink:label="loc_us-gaap_LiabilitiesCurrent_1477e02d-b6d8-3df6-e715-8364fedce6d9" xlink:type="locator" />
    <calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_ae9fd5e7-8741-dfbd-241e-1460ec6c786e" xlink:to="loc_us-gaap_LiabilitiesCurrent_1477e02d-b6d8-3df6-e715-8364fedce6d9" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_LongTermDebtNoncurrent" xlink:label="loc_us-gaap_LongTermDebtNoncurrent_89b83a57-ffbc-fc48-170d-f8b25d163e54" xlink:type="locator" />
    <calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_ae9fd5e7-8741-dfbd-241e-1460ec6c786e" xlink:to="loc_us-gaap_LongTermDebtNoncurrent_89b83a57-ffbc-fc48-170d-f8b25d163e54" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_DeferredTaxLiabilitiesNoncurrent" xlink:label="loc_us-gaap_DeferredTaxLiabilitiesNoncurrent_43aaac76-959d-69e2-234b-7c7a89d77345" xlink:type="locator" />
    <calculationArc order="3" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_ae9fd5e7-8741-dfbd-241e-1460ec6c786e" xlink:to="loc_us-gaap_DeferredTaxLiabilitiesNoncurrent_43aaac76-959d-69e2-234b-7c7a89d77345" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_DeferredRevenueNoncurrent" xlink:label="loc_us-gaap_DeferredRevenueNoncurrent_e71e7b37-2828-6759-f8ce-a5ac289c852b" xlink:type="locator" />
    <calculationArc order="4" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_ae9fd5e7-8741-dfbd-241e-1460ec6c786e" xlink:to="loc_us-gaap_DeferredRevenueNoncurrent_e71e7b37-2828-6759-f8ce-a5ac289c852b" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_OtherLiabilitiesNoncurrent" xlink:label="loc_us-gaap_OtherLiabilitiesNoncurrent_f7fce5cd-2d53-a8b3-a485-6d0f3635b8fe" xlink:type="locator" />
    <calculationArc order="5" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_ae9fd5e7-8741-dfbd-241e-1460ec6c786e" xlink:to="loc_us-gaap_OtherLiabilitiesNoncurrent_f7fce5cd-2d53-a8b3-a485-6d0f3635b8fe" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_CommitmentsAndContingencies" xlink:label="loc_us-gaap_CommitmentsAndContingencies_a7bb915d-e645-20d7-7b2e-623731454f45" xlink:type="locator" />
    <calculationArc order="6" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_ae9fd5e7-8741-dfbd-241e-1460ec6c786e" xlink:to="loc_us-gaap_CommitmentsAndContingencies_a7bb915d-e645-20d7-7b2e-623731454f45" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_StockholdersEquity" xlink:label="loc_us-gaap_StockholdersEquity_26e56c7a-24ab-e869-809a-0509ccbe86d9" xlink:type="locator" />
    <calculationArc order="7" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_ae9fd5e7-8741-dfbd-241e-1460ec6c786e" xlink:to="loc_us-gaap_StockholdersEquity_26e56c7a-24ab-e869-809a-0509ccbe86d9" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_PreferredStockValue" xlink:label="loc_us-gaap_PreferredStockValue_9fd7dfd7-596e-4c7f-4077-abbf8130d157" xlink:type="locator" />
    <calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_26e56c7a-24ab-e869-809a-0509ccbe86d9" xlink:to="loc_us-gaap_PreferredStockValue_9fd7dfd7-596e-4c7f-4077-abbf8130d157" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_CommonStockValue" xlink:label="loc_us-gaap_CommonStockValue_ffd09989-75a8-4713-beb0-21bebb3561d6" xlink:type="locator" />
    <calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_26e56c7a-24ab-e869-809a-0509ccbe86d9" xlink:to="loc_us-gaap_CommonStockValue_ffd09989-75a8-4713-beb0-21bebb3561d6" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_AdditionalPaidInCapital" xlink:label="loc_us-gaap_AdditionalPaidInCapital_dcbd5d14-4ec6-06fe-e5c1-a41efced6761" xlink:type="locator" />
    <calculationArc order="3" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_26e56c7a-24ab-e869-809a-0509ccbe86d9" xlink:to="loc_us-gaap_AdditionalPaidInCapital_dcbd5d14-4ec6-06fe-e5c1-a41efced6761" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_RetainedEarningsAccumulatedDeficit" xlink:label="loc_us-gaap_RetainedEarningsAccumulatedDeficit_440a13e9-e4dc-f397-4d0d-27daacd44483" xlink:type="locator" />
    <calculationArc order="4" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_26e56c7a-24ab-e869-809a-0509ccbe86d9" xlink:to="loc_us-gaap_RetainedEarningsAccumulatedDeficit_440a13e9-e4dc-f397-4d0d-27daacd44483" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_4d60b95b-a2a9-55da-7eaa-2ca718a58484" xlink:type="locator" />
    <calculationArc order="5" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_26e56c7a-24ab-e869-809a-0509ccbe86d9" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_4d60b95b-a2a9-55da-7eaa-2ca718a58484" xlink:type="arc" />
    <calculationArc order="6" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_26e56c7a-24ab-e869-809a-0509ccbe86d9" xlink:to="loc_us-gaap_CommitmentsAndContingencies_a7bb915d-e645-20d7-7b2e-623731454f45" xlink:type="arc" />
  </calculationLink>
  <calculationLink xlink:role="http://www.hologic.com/role/ConsolidatedBalanceSheetsParenthetical" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/ConsolidatedStatementsOfCashFlows" xlink:type="extended">
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_CashAndCashEquivalentsPeriodIncreaseDecrease" xlink:label="loc_us-gaap_CashAndCashEquivalentsPeriodIncreaseDecrease_46b895b2-8ff5-9e37-d4c3-5b75649cbcba" xlink:type="locator" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_b6b5c2f7-2ab7-a264-6d06-119dda52768c" xlink:type="locator" />
    <calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashAndCashEquivalentsPeriodIncreaseDecrease_46b895b2-8ff5-9e37-d4c3-5b75649cbcba" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_b6b5c2f7-2ab7-a264-6d06-119dda52768c" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_NetIncomeLoss" xlink:label="loc_us-gaap_NetIncomeLoss_faecc408-4bea-6e13-83bd-71b4c2ddf64c" xlink:type="locator" />
    <calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_b6b5c2f7-2ab7-a264-6d06-119dda52768c" xlink:to="loc_us-gaap_NetIncomeLoss_faecc408-4bea-6e13-83bd-71b4c2ddf64c" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_Depreciation" xlink:label="loc_us-gaap_Depreciation_296ac298-3acc-b888-34a0-99e6da446889" xlink:type="locator" />
    <calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_b6b5c2f7-2ab7-a264-6d06-119dda52768c" xlink:to="loc_us-gaap_Depreciation_296ac298-3acc-b888-34a0-99e6da446889" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_AdjustmentForAmortization" xlink:label="loc_us-gaap_AdjustmentForAmortization_4a5e87d4-523a-2cb0-e233-40027cfff53a" xlink:type="locator" />
    <calculationArc order="3" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_b6b5c2f7-2ab7-a264-6d06-119dda52768c" xlink:to="loc_us-gaap_AdjustmentForAmortization_4a5e87d4-523a-2cb0-e233-40027cfff53a" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_AmortizationOfFinancingCostsAndDiscounts" xlink:label="loc_us-gaap_AmortizationOfFinancingCostsAndDiscounts_cad4dd20-f895-7d13-fef5-2f2bffdf14ac" xlink:type="locator" />
    <calculationArc order="4" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_b6b5c2f7-2ab7-a264-6d06-119dda52768c" xlink:to="loc_us-gaap_AmortizationOfFinancingCostsAndDiscounts_cad4dd20-f895-7d13-fef5-2f2bffdf14ac" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_ShareBasedCompensation" xlink:label="loc_us-gaap_ShareBasedCompensation_aa7a0bc5-86c6-13a8-3676-9960bad8007f" xlink:type="locator" />
    <calculationArc order="5" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_b6b5c2f7-2ab7-a264-6d06-119dda52768c" xlink:to="loc_us-gaap_ShareBasedCompensation_aa7a0bc5-86c6-13a8-3676-9960bad8007f" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_ExcessTaxBenefitFromShareBasedCompensationOperatingActivities" xlink:label="loc_us-gaap_ExcessTaxBenefitFromShareBasedCompensationOperatingActivities_7af8c3cd-de8c-3d70-c017-7b54f4e9ba21" xlink:type="locator" />
    <calculationArc order="6" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_b6b5c2f7-2ab7-a264-6d06-119dda52768c" xlink:to="loc_us-gaap_ExcessTaxBenefitFromShareBasedCompensationOperatingActivities_7af8c3cd-de8c-3d70-c017-7b54f4e9ba21" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_DeferredIncomeTaxExpenseBenefit" xlink:label="loc_us-gaap_DeferredIncomeTaxExpenseBenefit_b14e4ab6-e003-13d4-eacd-26899afc8b93" xlink:type="locator" />
    <calculationArc order="7" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_b6b5c2f7-2ab7-a264-6d06-119dda52768c" xlink:to="loc_us-gaap_DeferredIncomeTaxExpenseBenefit_b14e4ab6-e003-13d4-eacd-26899afc8b93" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_AssetImpairmentCharges" xlink:label="loc_us-gaap_AssetImpairmentCharges_e0c47166-10e0-b00a-6c1c-0a652da2ca7e" xlink:type="locator" />
    <calculationArc order="8" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_b6b5c2f7-2ab7-a264-6d06-119dda52768c" xlink:to="loc_us-gaap_AssetImpairmentCharges_e0c47166-10e0-b00a-6c1c-0a652da2ca7e" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_GainsLossesOnExtinguishmentOfDebt" xlink:label="loc_us-gaap_GainsLossesOnExtinguishmentOfDebt_60dc6c6f-47c9-feed-cfc2-8fb0cfdc8ebf" xlink:type="locator" />
    <calculationArc order="9" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_b6b5c2f7-2ab7-a264-6d06-119dda52768c" xlink:to="loc_us-gaap_GainsLossesOnExtinguishmentOfDebt_60dc6c6f-47c9-feed-cfc2-8fb0cfdc8ebf" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_GainLossOnSaleOfPropertyPlantEquipment" xlink:label="loc_us-gaap_GainLossOnSaleOfPropertyPlantEquipment_f5bd4a60-d3d0-3020-79b0-0aaf08ea7fa1" xlink:type="locator" />
    <calculationArc order="10" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_b6b5c2f7-2ab7-a264-6d06-119dda52768c" xlink:to="loc_us-gaap_GainLossOnSaleOfPropertyPlantEquipment_f5bd4a60-d3d0-3020-79b0-0aaf08ea7fa1" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_OtherNoncashIncomeExpense" xlink:label="loc_us-gaap_OtherNoncashIncomeExpense_98d55fce-5941-51e9-8b41-6d9bb1d46d8b" xlink:type="locator" />
    <calculationArc order="11" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_b6b5c2f7-2ab7-a264-6d06-119dda52768c" xlink:to="loc_us-gaap_OtherNoncashIncomeExpense_98d55fce-5941-51e9-8b41-6d9bb1d46d8b" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_IncreaseDecreaseInAccountsReceivable" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsReceivable_fc73bf45-b7cf-c894-bf3a-f56c9d39cbe4" xlink:type="locator" />
    <calculationArc order="12" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_b6b5c2f7-2ab7-a264-6d06-119dda52768c" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsReceivable_fc73bf45-b7cf-c894-bf3a-f56c9d39cbe4" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_IncreaseDecreaseInInventories" xlink:label="loc_us-gaap_IncreaseDecreaseInInventories_d2a8fa0f-43df-513c-7a7b-93e04034bb1e" xlink:type="locator" />
    <calculationArc order="13" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_b6b5c2f7-2ab7-a264-6d06-119dda52768c" xlink:to="loc_us-gaap_IncreaseDecreaseInInventories_d2a8fa0f-43df-513c-7a7b-93e04034bb1e" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_IncreaseDecreaseInPrepaidTaxes" xlink:label="loc_us-gaap_IncreaseDecreaseInPrepaidTaxes_983a225f-02d6-d729-6fba-02aa0c5e86f1" xlink:type="locator" />
    <calculationArc order="14" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_b6b5c2f7-2ab7-a264-6d06-119dda52768c" xlink:to="loc_us-gaap_IncreaseDecreaseInPrepaidTaxes_983a225f-02d6-d729-6fba-02aa0c5e86f1" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets" xlink:label="loc_us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets_07d730ea-b32b-956c-677e-cf5b73c388b0" xlink:type="locator" />
    <calculationArc order="15" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_b6b5c2f7-2ab7-a264-6d06-119dda52768c" xlink:to="loc_us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets_07d730ea-b32b-956c-677e-cf5b73c388b0" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_IncreaseDecreaseInAccountsPayable" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsPayable_b9fb8522-752c-3209-8cca-60e64f420ad5" xlink:type="locator" />
    <calculationArc order="16" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_b6b5c2f7-2ab7-a264-6d06-119dda52768c" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsPayable_b9fb8522-752c-3209-8cca-60e64f420ad5" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_IncreaseDecreaseInAccruedLiabilities" xlink:label="loc_us-gaap_IncreaseDecreaseInAccruedLiabilities_5cb9a7e8-dd26-27c7-9313-c478703eadea" xlink:type="locator" />
    <calculationArc order="17" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_b6b5c2f7-2ab7-a264-6d06-119dda52768c" xlink:to="loc_us-gaap_IncreaseDecreaseInAccruedLiabilities_5cb9a7e8-dd26-27c7-9313-c478703eadea" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_IncreaseDecreaseInDeferredRevenue" xlink:label="loc_us-gaap_IncreaseDecreaseInDeferredRevenue_54734d8c-7af1-eb6f-7e56-5940fde2abb1" xlink:type="locator" />
    <calculationArc order="18" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_b6b5c2f7-2ab7-a264-6d06-119dda52768c" xlink:to="loc_us-gaap_IncreaseDecreaseInDeferredRevenue_54734d8c-7af1-eb6f-7e56-5940fde2abb1" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_fb013d5f-7e0c-1a40-5a42-42bd8c3a1d51" xlink:type="locator" />
    <calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashAndCashEquivalentsPeriodIncreaseDecrease_46b895b2-8ff5-9e37-d4c3-5b75649cbcba" xlink:to="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_fb013d5f-7e0c-1a40-5a42-42bd8c3a1d51" xlink:type="arc" />
    <loc xlink:href="holx-20150328.xsd#holx_ProceedsFromSaleOfBusiness" xlink:label="loc_holx_ProceedsFromSaleOfBusiness_997ea33d-10fb-7ad5-da78-9b2219009b94" xlink:type="locator" />
    <calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_fb013d5f-7e0c-1a40-5a42-42bd8c3a1d51" xlink:to="loc_holx_ProceedsFromSaleOfBusiness_997ea33d-10fb-7ad5-da78-9b2219009b94" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_PaymentsToAcquirePropertyPlantAndEquipment" xlink:label="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_37181af6-e562-cefc-f2b6-8490ea2c3e04" xlink:type="locator" />
    <calculationArc order="2" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_fb013d5f-7e0c-1a40-5a42-42bd8c3a1d51" xlink:to="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_37181af6-e562-cefc-f2b6-8490ea2c3e04" xlink:type="arc" />
    <loc xlink:href="holx-20150328.xsd#holx_PaymentsToManufactureEquipmentUnderCustomerUsageAgreements" xlink:label="loc_holx_PaymentsToManufactureEquipmentUnderCustomerUsageAgreements_3ad3764a-2138-bbbe-cf5e-9b221b9fc7ad" xlink:type="locator" />
    <calculationArc order="3" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_fb013d5f-7e0c-1a40-5a42-42bd8c3a1d51" xlink:to="loc_holx_PaymentsToManufactureEquipmentUnderCustomerUsageAgreements_3ad3764a-2138-bbbe-cf5e-9b221b9fc7ad" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_PaymentsForProceedsFromLifeInsurancePolicies" xlink:label="loc_us-gaap_PaymentsForProceedsFromLifeInsurancePolicies_152ca3e8-106a-e9ae-5130-2b990ea4395c" xlink:type="locator" />
    <calculationArc order="4" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_fb013d5f-7e0c-1a40-5a42-42bd8c3a1d51" xlink:to="loc_us-gaap_PaymentsForProceedsFromLifeInsurancePolicies_152ca3e8-106a-e9ae-5130-2b990ea4395c" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_PaymentsToAcquireTradingSecuritiesHeldforinvestment" xlink:label="loc_us-gaap_PaymentsToAcquireTradingSecuritiesHeldforinvestment_985c437c-74bd-b577-7dc4-5eaddff64bd0" xlink:type="locator" />
    <calculationArc order="5" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_fb013d5f-7e0c-1a40-5a42-42bd8c3a1d51" xlink:to="loc_us-gaap_PaymentsToAcquireTradingSecuritiesHeldforinvestment_985c437c-74bd-b577-7dc4-5eaddff64bd0" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_ProceedsFromPaymentsForTradingSecurities" xlink:label="loc_us-gaap_ProceedsFromPaymentsForTradingSecurities_f4e62e8a-1d67-b69f-d541-2e2406dc0a2a" xlink:type="locator" />
    <calculationArc order="6" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_fb013d5f-7e0c-1a40-5a42-42bd8c3a1d51" xlink:to="loc_us-gaap_ProceedsFromPaymentsForTradingSecurities_f4e62e8a-1d67-b69f-d541-2e2406dc0a2a" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_IncreaseDecreaseInOtherNoncurrentAssets" xlink:label="loc_us-gaap_IncreaseDecreaseInOtherNoncurrentAssets_61010cf2-f381-68a2-d0b7-2785316c9e09" xlink:type="locator" />
    <calculationArc order="7" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_fb013d5f-7e0c-1a40-5a42-42bd8c3a1d51" xlink:to="loc_us-gaap_IncreaseDecreaseInOtherNoncurrentAssets_61010cf2-f381-68a2-d0b7-2785316c9e09" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_6d7b73b6-0db7-23f7-377e-417fa8fc8e27" xlink:type="locator" />
    <calculationArc order="3" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashAndCashEquivalentsPeriodIncreaseDecrease_46b895b2-8ff5-9e37-d4c3-5b75649cbcba" xlink:to="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_6d7b73b6-0db7-23f7-377e-417fa8fc8e27" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_RepaymentsOfLongTermDebt" xlink:label="loc_us-gaap_RepaymentsOfLongTermDebt_555855ee-67fc-e384-4c0f-c30d34ed47e5" xlink:type="locator" />
    <calculationArc order="1" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_6d7b73b6-0db7-23f7-377e-417fa8fc8e27" xlink:to="loc_us-gaap_RepaymentsOfLongTermDebt_555855ee-67fc-e384-4c0f-c30d34ed47e5" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_PaymentsOfDebtIssuanceCosts" xlink:label="loc_us-gaap_PaymentsOfDebtIssuanceCosts_f2f70b98-dc3e-2bfa-ea39-be3a734476b9" xlink:type="locator" />
    <calculationArc order="2" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_6d7b73b6-0db7-23f7-377e-417fa8fc8e27" xlink:to="loc_us-gaap_PaymentsOfDebtIssuanceCosts_f2f70b98-dc3e-2bfa-ea39-be3a734476b9" xlink:type="arc" />
    <loc xlink:href="holx-20150328.xsd#holx_InterestRateCapAgreementsAggregatePremiumPayable" xlink:label="loc_holx_InterestRateCapAgreementsAggregatePremiumPayable_4bc6da72-e1bb-4e34-49b3-dc851860b4ab" xlink:type="locator" />
    <calculationArc order="3" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_6d7b73b6-0db7-23f7-377e-417fa8fc8e27" xlink:to="loc_holx_InterestRateCapAgreementsAggregatePremiumPayable_4bc6da72-e1bb-4e34-49b3-dc851860b4ab" xlink:type="arc" />
    <loc xlink:href="holx-20150328.xsd#holx_PaymentOfDeferredAcquisitionConsideration" xlink:label="loc_holx_PaymentOfDeferredAcquisitionConsideration_fd4b6c8f-bc1d-c2eb-7a60-9b221aae4aaf" xlink:type="locator" />
    <calculationArc order="4" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_6d7b73b6-0db7-23f7-377e-417fa8fc8e27" xlink:to="loc_holx_PaymentOfDeferredAcquisitionConsideration_fd4b6c8f-bc1d-c2eb-7a60-9b221aae4aaf" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_ProceedsFromIssuanceOfSharesUnderIncentiveAndShareBasedCompensationPlansIncludingStockOptions" xlink:label="loc_us-gaap_ProceedsFromIssuanceOfSharesUnderIncentiveAndShareBasedCompensationPlansIncludingStockOptions_2d132af0-7f39-d203-f615-6551f8d2a6a7" xlink:type="locator" />
    <calculationArc order="5" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_6d7b73b6-0db7-23f7-377e-417fa8fc8e27" xlink:to="loc_us-gaap_ProceedsFromIssuanceOfSharesUnderIncentiveAndShareBasedCompensationPlansIncludingStockOptions_2d132af0-7f39-d203-f615-6551f8d2a6a7" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_ExcessTaxBenefitFromShareBasedCompensationFinancingActivities" xlink:label="loc_us-gaap_ExcessTaxBenefitFromShareBasedCompensationFinancingActivities_9e5484cd-8eeb-f906-2a82-95e418e07947" xlink:type="locator" />
    <calculationArc order="6" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_6d7b73b6-0db7-23f7-377e-417fa8fc8e27" xlink:to="loc_us-gaap_ExcessTaxBenefitFromShareBasedCompensationFinancingActivities_9e5484cd-8eeb-f906-2a82-95e418e07947" xlink:type="arc" />
    <loc xlink:href="holx-20150328.xsd#holx_PaymentForTaxWithholdingRelatedToVestedAndReleasedRestrictedStockUnits" xlink:label="loc_holx_PaymentForTaxWithholdingRelatedToVestedAndReleasedRestrictedStockUnits_2e08713f-845a-5d6b-4179-9b221aebbe73" xlink:type="locator" />
    <calculationArc order="7" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_6d7b73b6-0db7-23f7-377e-417fa8fc8e27" xlink:to="loc_holx_PaymentForTaxWithholdingRelatedToVestedAndReleasedRestrictedStockUnits_2e08713f-845a-5d6b-4179-9b221aebbe73" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_EffectOfExchangeRateOnCashAndCashEquivalents" xlink:label="loc_us-gaap_EffectOfExchangeRateOnCashAndCashEquivalents_21149c4c-60e9-1f3a-82d3-665a83a5c4f2" xlink:type="locator" />
    <calculationArc order="4" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashAndCashEquivalentsPeriodIncreaseDecrease_46b895b2-8ff5-9e37-d4c3-5b75649cbcba" xlink:to="loc_us-gaap_EffectOfExchangeRateOnCashAndCashEquivalents_21149c4c-60e9-1f3a-82d3-665a83a5c4f2" xlink:type="arc" />
  </calculationLink>
  <calculationLink xlink:role="http://www.hologic.com/role/ConsolidatedStatementsOfComprehensiveIncomeLoss" xlink:type="extended">
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_ComprehensiveIncomeNetOfTax" xlink:label="loc_us-gaap_ComprehensiveIncomeNetOfTax_375e0d17-4818-4239-a370-aada0b6ca8eb" xlink:type="locator" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_NetIncomeLoss" xlink:label="loc_us-gaap_NetIncomeLoss_faecc408-4bea-6e13-83bd-71b4c2ddf64c" xlink:type="locator" />
    <calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_375e0d17-4818-4239-a370-aada0b6ca8eb" xlink:to="loc_us-gaap_NetIncomeLoss_faecc408-4bea-6e13-83bd-71b4c2ddf64c" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_c0a922b8-d3f5-9b5b-13d6-ad8882d39e41" xlink:type="locator" />
    <calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_375e0d17-4818-4239-a370-aada0b6ca8eb" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_c0a922b8-d3f5-9b5b-13d6-ad8882d39e41" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_OtherComprehensiveIncomeForeignCurrencyTransactionAndTranslationAdjustmentNetOfTaxPortionAttributableToParent" xlink:label="loc_us-gaap_OtherComprehensiveIncomeForeignCurrencyTransactionAndTranslationAdjustmentNetOfTaxPortionAttributableToParent_f949b2e8-d740-7c2b-d949-74ff04888c67" xlink:type="locator" />
    <calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_c0a922b8-d3f5-9b5b-13d6-ad8882d39e41" xlink:to="loc_us-gaap_OtherComprehensiveIncomeForeignCurrencyTransactionAndTranslationAdjustmentNetOfTaxPortionAttributableToParent_f949b2e8-d740-7c2b-d949-74ff04888c67" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_OtherComprehensiveIncomeLossAvailableForSaleSecuritiesAdjustmentNetOfTax" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossAvailableForSaleSecuritiesAdjustmentNetOfTax_9cf69aee-515f-6301-13fd-c65343678f68" xlink:type="locator" />
    <calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_c0a922b8-d3f5-9b5b-13d6-ad8882d39e41" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossAvailableForSaleSecuritiesAdjustmentNetOfTax_9cf69aee-515f-6301-13fd-c65343678f68" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_OtherComprehensiveIncomeDefinedBenefitPlansAdjustmentNetOfTaxPortionAttributableToParent" xlink:label="loc_us-gaap_OtherComprehensiveIncomeDefinedBenefitPlansAdjustmentNetOfTaxPortionAttributableToParent_e79edc95-de47-7f7d-36d1-71722214678b" xlink:type="locator" />
    <calculationArc order="3" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_c0a922b8-d3f5-9b5b-13d6-ad8882d39e41" xlink:to="loc_us-gaap_OtherComprehensiveIncomeDefinedBenefitPlansAdjustmentNetOfTaxPortionAttributableToParent_e79edc95-de47-7f7d-36d1-71722214678b" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_DerivativeInstrumentsGainLossRecognizedInOtherComprehensiveIncomeEffectivePortionNet" xlink:label="loc_us-gaap_DerivativeInstrumentsGainLossRecognizedInOtherComprehensiveIncomeEffectivePortionNet_94c12879-3943-ab4e-516d-23e8930780d9" xlink:type="locator" />
    <calculationArc order="4" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_c0a922b8-d3f5-9b5b-13d6-ad8882d39e41" xlink:to="loc_us-gaap_DerivativeInstrumentsGainLossRecognizedInOtherComprehensiveIncomeEffectivePortionNet_94c12879-3943-ab4e-516d-23e8930780d9" xlink:type="arc" />
  </calculationLink>
  <calculationLink xlink:role="http://www.hologic.com/role/ConsolidatedStatementsOfComprehensiveIncomeLossParenthetical" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/ConsolidatedStatementsOfOperations" xlink:type="extended">
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesMinorityInterestAndIncomeLossFromEquityMethodInvestments" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesMinorityInterestAndIncomeLossFromEquityMethodInvestments_10afc0e9-1faa-0be3-fc32-15d128e46f3f" xlink:type="locator" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_OperatingIncomeLoss" xlink:label="loc_us-gaap_OperatingIncomeLoss_72b2c986-6e1a-bdde-cc72-888b0df562b9" xlink:type="locator" />
    <calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesMinorityInterestAndIncomeLossFromEquityMethodInvestments_10afc0e9-1faa-0be3-fc32-15d128e46f3f" xlink:to="loc_us-gaap_OperatingIncomeLoss_72b2c986-6e1a-bdde-cc72-888b0df562b9" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_GrossProfit" xlink:label="loc_us-gaap_GrossProfit_77a77c8a-8a13-919c-6d50-d35f60384ab0" xlink:type="locator" />
    <calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingIncomeLoss_72b2c986-6e1a-bdde-cc72-888b0df562b9" xlink:to="loc_us-gaap_GrossProfit_77a77c8a-8a13-919c-6d50-d35f60384ab0" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_CostOfGoodsSold" xlink:label="loc_us-gaap_CostOfGoodsSold_f60bb493-e4bb-89cc-3640-4febfc5d9f27" xlink:type="locator" />
    <calculationArc order="1" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_GrossProfit_77a77c8a-8a13-919c-6d50-d35f60384ab0" xlink:to="loc_us-gaap_CostOfGoodsSold_f60bb493-e4bb-89cc-3640-4febfc5d9f27" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_CostOfGoodsSoldAmortization" xlink:label="loc_us-gaap_CostOfGoodsSoldAmortization_0d1203ac-2d54-3546-2b4c-40da83ecd7f1" xlink:type="locator" />
    <calculationArc order="2" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_GrossProfit_77a77c8a-8a13-919c-6d50-d35f60384ab0" xlink:to="loc_us-gaap_CostOfGoodsSoldAmortization_0d1203ac-2d54-3546-2b4c-40da83ecd7f1" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_CostOfServices" xlink:label="loc_us-gaap_CostOfServices_a6952e48-bc96-ff61-82b1-8e883f0735ee" xlink:type="locator" />
    <calculationArc order="3" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_GrossProfit_77a77c8a-8a13-919c-6d50-d35f60384ab0" xlink:to="loc_us-gaap_CostOfServices_a6952e48-bc96-ff61-82b1-8e883f0735ee" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_Revenues" xlink:label="loc_us-gaap_Revenues_467b5ef7-2b02-661a-3e41-f79140739819" xlink:type="locator" />
    <calculationArc order="4" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_GrossProfit_77a77c8a-8a13-919c-6d50-d35f60384ab0" xlink:to="loc_us-gaap_Revenues_467b5ef7-2b02-661a-3e41-f79140739819" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_SalesRevenueGoodsNet" xlink:label="loc_us-gaap_SalesRevenueGoodsNet_407dba67-ed61-44c5-d72f-b6457c9a2967" xlink:type="locator" />
    <calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Revenues_467b5ef7-2b02-661a-3e41-f79140739819" xlink:to="loc_us-gaap_SalesRevenueGoodsNet_407dba67-ed61-44c5-d72f-b6457c9a2967" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_SalesRevenueServicesNet" xlink:label="loc_us-gaap_SalesRevenueServicesNet_8bcef5d0-be55-1817-9796-00fa51e44801" xlink:type="locator" />
    <calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Revenues_467b5ef7-2b02-661a-3e41-f79140739819" xlink:to="loc_us-gaap_SalesRevenueServicesNet_8bcef5d0-be55-1817-9796-00fa51e44801" xlink:type="arc" />
    <loc xlink:href="holx-20150328.xsd#holx_CostOfGoodsSoldImpairmentOfIntangibleAssets" xlink:label="loc_holx_CostOfGoodsSoldImpairmentOfIntangibleAssets_91a502a2-c76f-0a57-6727-9b221b81fa10" xlink:type="locator" />
    <calculationArc order="5" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_GrossProfit_77a77c8a-8a13-919c-6d50-d35f60384ab0" xlink:to="loc_holx_CostOfGoodsSoldImpairmentOfIntangibleAssets_91a502a2-c76f-0a57-6727-9b221b81fa10" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_OperatingExpenses" xlink:label="loc_us-gaap_OperatingExpenses_ee2bd36d-150a-7303-38c4-eb755f792210" xlink:type="locator" />
    <calculationArc order="2" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingIncomeLoss_72b2c986-6e1a-bdde-cc72-888b0df562b9" xlink:to="loc_us-gaap_OperatingExpenses_ee2bd36d-150a-7303-38c4-eb755f792210" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_ResearchAndDevelopmentExpense" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpense_7d1bc05d-33ee-c009-4533-b70ee0a74dc9" xlink:type="locator" />
    <calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingExpenses_ee2bd36d-150a-7303-38c4-eb755f792210" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpense_7d1bc05d-33ee-c009-4533-b70ee0a74dc9" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_SellingAndMarketingExpense" xlink:label="loc_us-gaap_SellingAndMarketingExpense_719efaf7-afa3-6714-cbd0-f73f03530dfc" xlink:type="locator" />
    <calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingExpenses_ee2bd36d-150a-7303-38c4-eb755f792210" xlink:to="loc_us-gaap_SellingAndMarketingExpense_719efaf7-afa3-6714-cbd0-f73f03530dfc" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_GeneralAndAdministrativeExpense" xlink:label="loc_us-gaap_GeneralAndAdministrativeExpense_7c6d12de-ee49-8fea-ba36-2f44ccb8dae4" xlink:type="locator" />
    <calculationArc order="3" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingExpenses_ee2bd36d-150a-7303-38c4-eb755f792210" xlink:to="loc_us-gaap_GeneralAndAdministrativeExpense_7c6d12de-ee49-8fea-ba36-2f44ccb8dae4" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_AmortizationOfIntangibleAssets" xlink:label="loc_us-gaap_AmortizationOfIntangibleAssets_c1b76359-2bf3-ac34-0e0e-8614c806c9a0" xlink:type="locator" />
    <calculationArc order="4" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingExpenses_ee2bd36d-150a-7303-38c4-eb755f792210" xlink:to="loc_us-gaap_AmortizationOfIntangibleAssets_c1b76359-2bf3-ac34-0e0e-8614c806c9a0" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_RestructuringCharges" xlink:label="loc_us-gaap_RestructuringCharges_970b1d52-2feb-0d82-6a9e-66cd8dd55db9" xlink:type="locator" />
    <calculationArc order="5" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingExpenses_ee2bd36d-150a-7303-38c4-eb755f792210" xlink:to="loc_us-gaap_RestructuringCharges_970b1d52-2feb-0d82-6a9e-66cd8dd55db9" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_ImpairmentOfIntangibleAssetsIndefinitelivedExcludingGoodwill" xlink:label="loc_us-gaap_ImpairmentOfIntangibleAssetsIndefinitelivedExcludingGoodwill_730d15bd-e861-ae20-5018-83aef5d9b08f" xlink:type="locator" />
    <calculationArc order="6" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingExpenses_ee2bd36d-150a-7303-38c4-eb755f792210" xlink:to="loc_us-gaap_ImpairmentOfIntangibleAssetsIndefinitelivedExcludingGoodwill_730d15bd-e861-ae20-5018-83aef5d9b08f" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_InvestmentIncomeInterest" xlink:label="loc_us-gaap_InvestmentIncomeInterest_a24d82ba-62b0-7744-0b3a-f37dbe668bee" xlink:type="locator" />
    <calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesMinorityInterestAndIncomeLossFromEquityMethodInvestments_10afc0e9-1faa-0be3-fc32-15d128e46f3f" xlink:to="loc_us-gaap_InvestmentIncomeInterest_a24d82ba-62b0-7744-0b3a-f37dbe668bee" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_InterestExpense" xlink:label="loc_us-gaap_InterestExpense_cefe1779-647b-11ee-28a9-2740d86c056b" xlink:type="locator" />
    <calculationArc order="3" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesMinorityInterestAndIncomeLossFromEquityMethodInvestments_10afc0e9-1faa-0be3-fc32-15d128e46f3f" xlink:to="loc_us-gaap_InterestExpense_cefe1779-647b-11ee-28a9-2740d86c056b" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_GainsLossesOnExtinguishmentOfDebt" xlink:label="loc_us-gaap_GainsLossesOnExtinguishmentOfDebt_60dc6c6f-47c9-feed-cfc2-8fb0cfdc8ebf" xlink:type="locator" />
    <calculationArc order="4" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesMinorityInterestAndIncomeLossFromEquityMethodInvestments_10afc0e9-1faa-0be3-fc32-15d128e46f3f" xlink:to="loc_us-gaap_GainsLossesOnExtinguishmentOfDebt_60dc6c6f-47c9-feed-cfc2-8fb0cfdc8ebf" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_OtherNonoperatingIncomeExpense" xlink:label="loc_us-gaap_OtherNonoperatingIncomeExpense_27b74f5e-bc16-8d0b-2d8b-f8fb77af5bf6" xlink:type="locator" />
    <calculationArc order="5" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesMinorityInterestAndIncomeLossFromEquityMethodInvestments_10afc0e9-1faa-0be3-fc32-15d128e46f3f" xlink:to="loc_us-gaap_OtherNonoperatingIncomeExpense_27b74f5e-bc16-8d0b-2d8b-f8fb77af5bf6" xlink:type="arc" />
  </calculationLink>
  <calculationLink xlink:role="http://www.hologic.com/role/DerivativesAdditionalInformationDetails" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/DerivativesNotes" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/DerivativesScheduleOfDerivativeInstrumentsOnConsolidatedBalanceSheetsAndUnrealizedLossRecognizedInAociDetails" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/DerivativesTables" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/DocumentAndEntityInformation" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/FairValueMeasurements" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/FairValueMeasurementsAdditionalInformationDetail" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/FairValueMeasurementsEstimatedFairValuesOfConvertibleNotesDetail" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/FairValueMeasurementsFairValueAssetsAndLiabilitiesMeasuredOnRecurringBasisDetail" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/FairValueMeasurementsTables" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/IncomeTaxes" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/IncomeTaxesAdditionalInformationDetail" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/IntangibleAssets" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/IntangibleAssetsAdditionalInformationDetail" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/IntangibleAssetsScheduleOfEstimatedAmortizationExpenseDetail" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/IntangibleAssetsScheduleOfIntangibleAssetsDetail" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/IntangibleAssetsTables" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/MarketableSecurities" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/MarketableSecuritiesReconcilesCostBasisToFairMarketValueOfCompanysEquitySecurityDetail" xlink:type="extended">
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_AvailableForSaleSecurities" xlink:label="loc_us-gaap_AvailableForSaleSecurities_181eb207-24a2-d4cc-f2e5-ac567c0506af" xlink:type="locator" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_AvailableForSaleEquitySecuritiesAmortizedCostBasis" xlink:label="loc_us-gaap_AvailableForSaleEquitySecuritiesAmortizedCostBasis_0bfa0f19-2b77-9a60-e98d-732b2a1d3611" xlink:type="locator" />
    <calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AvailableForSaleSecurities_181eb207-24a2-d4cc-f2e5-ac567c0506af" xlink:to="loc_us-gaap_AvailableForSaleEquitySecuritiesAmortizedCostBasis_0bfa0f19-2b77-9a60-e98d-732b2a1d3611" xlink:type="arc" />
    <loc xlink:href="holx-20150328.xsd#holx_AvailableForSaleSecuritiesGrossUnrealizedGainOne" xlink:label="loc_holx_AvailableForSaleSecuritiesGrossUnrealizedGainOne_74918d36-35cc-9cb4-97c3-9b221b95abc7" xlink:type="locator" />
    <calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AvailableForSaleSecurities_181eb207-24a2-d4cc-f2e5-ac567c0506af" xlink:to="loc_holx_AvailableForSaleSecuritiesGrossUnrealizedGainOne_74918d36-35cc-9cb4-97c3-9b221b95abc7" xlink:type="arc" />
    <loc xlink:href="holx-20150328.xsd#holx_AvailableForSaleSecuritiesGrossUnrealizedLossesTwo" xlink:label="loc_holx_AvailableForSaleSecuritiesGrossUnrealizedLossesTwo_ac2aaa38-1bbf-345c-06ef-9b221acd5c0f" xlink:type="locator" />
    <calculationArc order="3" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AvailableForSaleSecurities_181eb207-24a2-d4cc-f2e5-ac567c0506af" xlink:to="loc_holx_AvailableForSaleSecuritiesGrossUnrealizedLossesTwo_ac2aaa38-1bbf-345c-06ef-9b221acd5c0f" xlink:type="arc" />
  </calculationLink>
  <calculationLink xlink:role="http://www.hologic.com/role/MarketableSecuritiesTables" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/NetIncomeLossPerShare" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/NetIncomeLossPerShareReconciliationOfBasicAndDilutedShareAmountsDetail" xlink:type="extended">
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding" xlink:label="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_72491ff2-4ebf-cd12-4650-465a32156ef1" xlink:type="locator" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_WeightedAverageNumberOfSharesOutstandingBasic" xlink:label="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_0f40104d-c144-6dd5-056b-98f95661c23e" xlink:type="locator" />
    <calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_72491ff2-4ebf-cd12-4650-465a32156ef1" xlink:to="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_0f40104d-c144-6dd5-056b-98f95661c23e" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_IncrementalCommonSharesAttributableToShareBasedPaymentArrangements" xlink:label="loc_us-gaap_IncrementalCommonSharesAttributableToShareBasedPaymentArrangements_9f5e56ff-4bcb-0366-e02d-528f3927d0cf" xlink:type="locator" />
    <calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_72491ff2-4ebf-cd12-4650-465a32156ef1" xlink:to="loc_us-gaap_IncrementalCommonSharesAttributableToShareBasedPaymentArrangements_9f5e56ff-4bcb-0366-e02d-528f3927d0cf" xlink:type="arc" />
    <loc xlink:href="holx-20150328.xsd#holx_IncrementalCommonSharesAttributableToTheConversionOfDebtSecurities" xlink:label="loc_holx_IncrementalCommonSharesAttributableToTheConversionOfDebtSecurities_d7784751-35c3-2e24-2096-9b221c3fa97b" xlink:type="locator" />
    <calculationArc order="3" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_72491ff2-4ebf-cd12-4650-465a32156ef1" xlink:to="loc_holx_IncrementalCommonSharesAttributableToTheConversionOfDebtSecurities_d7784751-35c3-2e24-2096-9b221c3fa97b" xlink:type="arc" />
  </calculationLink>
  <calculationLink xlink:role="http://www.hologic.com/role/NetIncomeLossPerShareTables" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/NewAccountingPronouncements" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/NewAccountingPronouncementsPolicies" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/OtherBalanceSheetInformation" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/OtherBalanceSheetInformationOtherBalanceSheetInformationOfInventoriesDetail" xlink:type="extended">
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_InventoryNet" xlink:label="loc_us-gaap_InventoryNet_a00a52e0-8d97-7156-7079-2c628ca1666f" xlink:type="locator" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_InventoryRawMaterialsNetOfReserves" xlink:label="loc_us-gaap_InventoryRawMaterialsNetOfReserves_892cc3f8-a219-534f-343c-8bc143468526" xlink:type="locator" />
    <calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InventoryNet_a00a52e0-8d97-7156-7079-2c628ca1666f" xlink:to="loc_us-gaap_InventoryRawMaterialsNetOfReserves_892cc3f8-a219-534f-343c-8bc143468526" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_InventoryWorkInProcessNetOfReserves" xlink:label="loc_us-gaap_InventoryWorkInProcessNetOfReserves_7353f9f6-2726-9549-78fa-b65880c06d35" xlink:type="locator" />
    <calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InventoryNet_a00a52e0-8d97-7156-7079-2c628ca1666f" xlink:to="loc_us-gaap_InventoryWorkInProcessNetOfReserves_7353f9f6-2726-9549-78fa-b65880c06d35" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_InventoryFinishedGoodsNetOfReserves" xlink:label="loc_us-gaap_InventoryFinishedGoodsNetOfReserves_4e67cf4c-a7ae-3e8f-1690-516c17ec8100" xlink:type="locator" />
    <calculationArc order="3" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InventoryNet_a00a52e0-8d97-7156-7079-2c628ca1666f" xlink:to="loc_us-gaap_InventoryFinishedGoodsNetOfReserves_4e67cf4c-a7ae-3e8f-1690-516c17ec8100" xlink:type="arc" />
  </calculationLink>
  <calculationLink xlink:role="http://www.hologic.com/role/OtherBalanceSheetInformationOtherBalanceSheetInformationOfPropertyPlantAndEquipmentDetail" xlink:type="extended">
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_PropertyPlantAndEquipmentNet" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_a1eaafaf-055b-e423-85ef-4424fb2ef4e4" xlink:type="locator" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_PropertyPlantAndEquipmentGross" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentGross_c4121e9a-097d-7136-dbac-b93469330286" xlink:type="locator" />
    <calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentNet_a1eaafaf-055b-e423-85ef-4424fb2ef4e4" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentGross_c4121e9a-097d-7136-dbac-b93469330286" xlink:type="arc" />
    <loc xlink:href="holx-20150328.xsd#holx_ManufacturingEquipmentAndSoftware" xlink:label="loc_holx_ManufacturingEquipmentAndSoftware_f24f04c7-0461-e9ab-a57f-9b2218a60aa5" xlink:type="locator" />
    <calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentGross_c4121e9a-097d-7136-dbac-b93469330286" xlink:to="loc_holx_ManufacturingEquipmentAndSoftware_f24f04c7-0461-e9ab-a57f-9b2218a60aa5" xlink:type="arc" />
    <loc xlink:href="holx-20150328.xsd#holx_EquipmentUnderCustomerUsageAgreements" xlink:label="loc_holx_EquipmentUnderCustomerUsageAgreements_aced2e1f-1aa5-35e4-5fec-9b221a54b0aa" xlink:type="locator" />
    <calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentGross_c4121e9a-097d-7136-dbac-b93469330286" xlink:to="loc_holx_EquipmentUnderCustomerUsageAgreements_aced2e1f-1aa5-35e4-5fec-9b221a54b0aa" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_BuildingsAndImprovementsGross" xlink:label="loc_us-gaap_BuildingsAndImprovementsGross_8778840e-08c3-f591-0639-9fa09c7a0691" xlink:type="locator" />
    <calculationArc order="3" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentGross_c4121e9a-097d-7136-dbac-b93469330286" xlink:to="loc_us-gaap_BuildingsAndImprovementsGross_8778840e-08c3-f591-0639-9fa09c7a0691" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_LeaseholdImprovementsGross" xlink:label="loc_us-gaap_LeaseholdImprovementsGross_380cdb08-8027-86c9-df01-d95747c69b41" xlink:type="locator" />
    <calculationArc order="4" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentGross_c4121e9a-097d-7136-dbac-b93469330286" xlink:to="loc_us-gaap_LeaseholdImprovementsGross_380cdb08-8027-86c9-df01-d95747c69b41" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_Land" xlink:label="loc_us-gaap_Land_c85e7eda-0d43-8765-45fe-06d684354a94" xlink:type="locator" />
    <calculationArc order="5" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentGross_c4121e9a-097d-7136-dbac-b93469330286" xlink:to="loc_us-gaap_Land_c85e7eda-0d43-8765-45fe-06d684354a94" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_FurnitureAndFixturesGross" xlink:label="loc_us-gaap_FurnitureAndFixturesGross_dc2b42df-f998-acc8-baa5-ac2c40676775" xlink:type="locator" />
    <calculationArc order="6" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentGross_c4121e9a-097d-7136-dbac-b93469330286" xlink:to="loc_us-gaap_FurnitureAndFixturesGross_dc2b42df-f998-acc8-baa5-ac2c40676775" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment" xlink:label="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_2c0591e3-817a-5563-df1e-5c3ede33266d" xlink:type="locator" />
    <calculationArc order="2" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentNet_a1eaafaf-055b-e423-85ef-4424fb2ef4e4" xlink:to="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_2c0591e3-817a-5563-df1e-5c3ede33266d" xlink:type="arc" />
  </calculationLink>
  <calculationLink xlink:role="http://www.hologic.com/role/OtherBalanceSheetInformationTables" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/ProductWarranties" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/ProductWarrantiesProductWarrantyDetail" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/ProductWarrantiesTables" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/RestructuringAndDivestitureCharges" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/RestructuringAndDivestitureChargesAdditionalInformationDetail" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/RestructuringAndDivestitureChargesChargesTakenRelatedToAccruedRestructuringActionsDetail" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/RestructuringAndDivestitureChargesChargesTakenRelatedToRestructuringActionsDetail" xlink:type="extended">
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_RestructuringCharges" xlink:label="loc_us-gaap_RestructuringCharges_970b1d52-2feb-0d82-6a9e-66cd8dd55db9" xlink:type="locator" />
    <loc xlink:href="holx-20150328.xsd#holx_RestructuringChargesUnderExitOrDisposalPlan" xlink:label="loc_holx_RestructuringChargesUnderExitOrDisposalPlan_2e409963-1322-b550-1721-9b221a7c5149" xlink:type="locator" />
    <calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_RestructuringCharges_970b1d52-2feb-0d82-6a9e-66cd8dd55db9" xlink:to="loc_holx_RestructuringChargesUnderExitOrDisposalPlan_2e409963-1322-b550-1721-9b221a7c5149" xlink:type="arc" />
    <loc xlink:href="holx-20150328.xsd#holx_ReductionOfWorkforceExpenses" xlink:label="loc_holx_ReductionOfWorkforceExpenses_e88b10d0-24de-e430-16b3-9b221bbd3282" xlink:type="locator" />
    <calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_holx_RestructuringChargesUnderExitOrDisposalPlan_2e409963-1322-b550-1721-9b221a7c5149" xlink:to="loc_holx_ReductionOfWorkforceExpenses_e88b10d0-24de-e430-16b3-9b221bbd3282" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_RestructuringCostsAndAssetImpairmentCharges" xlink:label="loc_us-gaap_RestructuringCostsAndAssetImpairmentCharges_de536d0d-2ccc-f8ad-db81-796fc8fb79b0" xlink:type="locator" />
    <calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_holx_RestructuringChargesUnderExitOrDisposalPlan_2e409963-1322-b550-1721-9b221a7c5149" xlink:to="loc_us-gaap_RestructuringCostsAndAssetImpairmentCharges_de536d0d-2ccc-f8ad-db81-796fc8fb79b0" xlink:type="arc" />
    <loc xlink:href="holx-20150328.xsd#holx_FacilityClosureCosts" xlink:label="loc_holx_FacilityClosureCosts_dbb27aa6-de06-568a-a99e-9b221b6d422d" xlink:type="locator" />
    <calculationArc order="3" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_holx_RestructuringChargesUnderExitOrDisposalPlan_2e409963-1322-b550-1721-9b221a7c5149" xlink:to="loc_holx_FacilityClosureCosts_dbb27aa6-de06-568a-a99e-9b221b6d422d" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_OtherRestructuringCosts" xlink:label="loc_us-gaap_OtherRestructuringCosts_df105929-deb1-61d8-342d-03ab57966c5c" xlink:type="locator" />
    <calculationArc order="4" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_holx_RestructuringChargesUnderExitOrDisposalPlan_2e409963-1322-b550-1721-9b221a7c5149" xlink:to="loc_us-gaap_OtherRestructuringCosts_df105929-deb1-61d8-342d-03ab57966c5c" xlink:type="arc" />
    <loc xlink:href="holx-20150328.xsd#holx_DivestitureChargesNet" xlink:label="loc_holx_DivestitureChargesNet_b5ea785f-14ec-be62-187e-9b221c67486a" xlink:type="locator" />
    <calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_RestructuringCharges_970b1d52-2feb-0d82-6a9e-66cd8dd55db9" xlink:to="loc_holx_DivestitureChargesNet_b5ea785f-14ec-be62-187e-9b221c67486a" xlink:type="arc" />
  </calculationLink>
  <calculationLink xlink:role="http://www.hologic.com/role/RestructuringAndDivestitureChargesTables" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/StockBasedCompensation" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/StockBasedCompensationAdditionalInformationDetail" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/StockBasedCompensationStockBasedCompensationExpenseInConsolidatedStatementsOfOperationsDetail" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/StockBasedCompensationTables" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/StockBasedCompensationWeightedAverageAssumptionsUtilizedToValueStockOptionsDetail" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/SupplementalGuarantorCondensedConsolidatingFinancials" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/SupplementalGuarantorCondensedConsolidatingFinancialsAdditionalInformationDetail" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/SupplementalGuarantorCondensedConsolidatingFinancialsScheduleOfConsolidatingStatementOfCashFlowsDetail" xlink:type="extended" />
  <calculationLink xlink:role="http://www.hologic.com/role/SupplementalGuarantorCondensedConsolidatingFinancialsScheduleOfSupplementalCondensedConsolidatingBalanceSheetDetail" xlink:type="extended">
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_LiabilitiesAndStockholdersEquity" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquity_ae9fd5e7-8741-dfbd-241e-1460ec6c786e" xlink:type="locator" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_LiabilitiesCurrent" xlink:label="loc_us-gaap_LiabilitiesCurrent_1477e02d-b6d8-3df6-e715-8364fedce6d9" xlink:type="locator" />
    <calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_ae9fd5e7-8741-dfbd-241e-1460ec6c786e" xlink:to="loc_us-gaap_LiabilitiesCurrent_1477e02d-b6d8-3df6-e715-8364fedce6d9" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_LongTermDebtCurrent" xlink:label="loc_us-gaap_LongTermDebtCurrent_0601d697-1537-c3cb-caaf-11141ac92b39" xlink:type="locator" />
    <calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_1477e02d-b6d8-3df6-e715-8364fedce6d9" xlink:to="loc_us-gaap_LongTermDebtCurrent_0601d697-1537-c3cb-caaf-11141ac92b39" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_AccountsPayableCurrent" xlink:label="loc_us-gaap_AccountsPayableCurrent_bd7b63fa-09ae-1100-16fe-5ac027208548" xlink:type="locator" />
    <calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_1477e02d-b6d8-3df6-e715-8364fedce6d9" xlink:to="loc_us-gaap_AccountsPayableCurrent_bd7b63fa-09ae-1100-16fe-5ac027208548" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_AccruedLiabilitiesCurrent" xlink:label="loc_us-gaap_AccruedLiabilitiesCurrent_1d834322-984c-3ee2-a4a0-2dc90b007763" xlink:type="locator" />
    <calculationArc order="3" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_1477e02d-b6d8-3df6-e715-8364fedce6d9" xlink:to="loc_us-gaap_AccruedLiabilitiesCurrent_1d834322-984c-3ee2-a4a0-2dc90b007763" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_DeferredRevenueCurrent" xlink:label="loc_us-gaap_DeferredRevenueCurrent_c952180c-d37a-a6b0-3e18-a18cd8328eee" xlink:type="locator" />
    <calculationArc order="4" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_1477e02d-b6d8-3df6-e715-8364fedce6d9" xlink:to="loc_us-gaap_DeferredRevenueCurrent_c952180c-d37a-a6b0-3e18-a18cd8328eee" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_DeferredTaxLiabilitiesCurrent" xlink:label="loc_us-gaap_DeferredTaxLiabilitiesCurrent_712a848e-6493-088e-8a82-029610cac2c5" xlink:type="locator" />
    <calculationArc order="5" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_1477e02d-b6d8-3df6-e715-8364fedce6d9" xlink:to="loc_us-gaap_DeferredTaxLiabilitiesCurrent_712a848e-6493-088e-8a82-029610cac2c5" xlink:type="arc" />
    <loc xlink:href="holx-20150328.xsd#holx_IntercompanyPayables" xlink:label="loc_holx_IntercompanyPayables_3f1921c0-6836-7cd2-42a2-9b221b31022c" xlink:type="locator" />
    <calculationArc order="6" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_1477e02d-b6d8-3df6-e715-8364fedce6d9" xlink:to="loc_holx_IntercompanyPayables_3f1921c0-6836-7cd2-42a2-9b221b31022c" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_LongTermDebtNoncurrent" xlink:label="loc_us-gaap_LongTermDebtNoncurrent_89b83a57-ffbc-fc48-170d-f8b25d163e54" xlink:type="locator" />
    <calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_ae9fd5e7-8741-dfbd-241e-1460ec6c786e" xlink:to="loc_us-gaap_LongTermDebtNoncurrent_89b83a57-ffbc-fc48-170d-f8b25d163e54" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_DeferredTaxLiabilitiesNoncurrent" xlink:label="loc_us-gaap_DeferredTaxLiabilitiesNoncurrent_43aaac76-959d-69e2-234b-7c7a89d77345" xlink:type="locator" />
    <calculationArc order="3" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_ae9fd5e7-8741-dfbd-241e-1460ec6c786e" xlink:to="loc_us-gaap_DeferredTaxLiabilitiesNoncurrent_43aaac76-959d-69e2-234b-7c7a89d77345" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_DeferredRevenueNoncurrent" xlink:label="loc_us-gaap_DeferredRevenueNoncurrent_e71e7b37-2828-6759-f8ce-a5ac289c852b" xlink:type="locator" />
    <calculationArc order="4" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_ae9fd5e7-8741-dfbd-241e-1460ec6c786e" xlink:to="loc_us-gaap_DeferredRevenueNoncurrent_e71e7b37-2828-6759-f8ce-a5ac289c852b" xlink:type="arc" />
    <loc xlink:href="holx-20150328.xsd#holx_IntercompanyNotesPayables" xlink:label="loc_holx_IntercompanyNotesPayables_895c1cf3-3243-032f-edae-9b221a9ac5d5" xlink:type="locator" />
    <calculationArc order="5" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_ae9fd5e7-8741-dfbd-241e-1460ec6c786e" xlink:to="loc_holx_IntercompanyNotesPayables_895c1cf3-3243-032f-edae-9b221a9ac5d5" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_OtherLiabilitiesNoncurrent" xlink:label="loc_us-gaap_OtherLiabilitiesNoncurrent_f7fce5cd-2d53-a8b3-a485-6d0f3635b8fe" xlink:type="locator" />
    <calculationArc order="6" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_ae9fd5e7-8741-dfbd-241e-1460ec6c786e" xlink:to="loc_us-gaap_OtherLiabilitiesNoncurrent_f7fce5cd-2d53-a8b3-a485-6d0f3635b8fe" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_StockholdersEquity" xlink:label="loc_us-gaap_StockholdersEquity_26e56c7a-24ab-e869-809a-0509ccbe86d9" xlink:type="locator" />
    <calculationArc order="7" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_ae9fd5e7-8741-dfbd-241e-1460ec6c786e" xlink:to="loc_us-gaap_StockholdersEquity_26e56c7a-24ab-e869-809a-0509ccbe86d9" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_Assets" xlink:label="loc_us-gaap_Assets_b517a4f1-4597-b094-2608-75af660328e1" xlink:type="locator" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_AssetsCurrent" xlink:label="loc_us-gaap_AssetsCurrent_67f49e84-05fd-4b0b-ae5a-e9913b97cea2" xlink:type="locator" />
    <calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_b517a4f1-4597-b094-2608-75af660328e1" xlink:to="loc_us-gaap_AssetsCurrent_67f49e84-05fd-4b0b-ae5a-e9913b97cea2" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_999960f8-6702-f7e9-88df-fcb8021751b2" xlink:type="locator" />
    <calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_67f49e84-05fd-4b0b-ae5a-e9913b97cea2" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_999960f8-6702-f7e9-88df-fcb8021751b2" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_RestrictedCashAndCashEquivalentsAtCarryingValue" xlink:label="loc_us-gaap_RestrictedCashAndCashEquivalentsAtCarryingValue_59dbb0ce-8e17-487b-ae40-e3a584ab7a36" xlink:type="locator" />
    <calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_67f49e84-05fd-4b0b-ae5a-e9913b97cea2" xlink:to="loc_us-gaap_RestrictedCashAndCashEquivalentsAtCarryingValue_59dbb0ce-8e17-487b-ae40-e3a584ab7a36" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_AccountsReceivableNetCurrent" xlink:label="loc_us-gaap_AccountsReceivableNetCurrent_dc301a2a-78b3-2f7c-72b2-6d5ec18f9327" xlink:type="locator" />
    <calculationArc order="3" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_67f49e84-05fd-4b0b-ae5a-e9913b97cea2" xlink:to="loc_us-gaap_AccountsReceivableNetCurrent_dc301a2a-78b3-2f7c-72b2-6d5ec18f9327" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_InventoryNet" xlink:label="loc_us-gaap_InventoryNet_a00a52e0-8d97-7156-7079-2c628ca1666f" xlink:type="locator" />
    <calculationArc order="4" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_67f49e84-05fd-4b0b-ae5a-e9913b97cea2" xlink:to="loc_us-gaap_InventoryNet_a00a52e0-8d97-7156-7079-2c628ca1666f" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_DeferredTaxAssetsNetCurrent" xlink:label="loc_us-gaap_DeferredTaxAssetsNetCurrent_963972b6-b12a-c8bc-5df9-cadb35399122" xlink:type="locator" />
    <calculationArc order="5" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_67f49e84-05fd-4b0b-ae5a-e9913b97cea2" xlink:to="loc_us-gaap_DeferredTaxAssetsNetCurrent_963972b6-b12a-c8bc-5df9-cadb35399122" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_PrepaidTaxes" xlink:label="loc_us-gaap_PrepaidTaxes_8ad6eab9-4262-f34c-4384-fcccebc59d02" xlink:type="locator" />
    <calculationArc order="6" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_67f49e84-05fd-4b0b-ae5a-e9913b97cea2" xlink:to="loc_us-gaap_PrepaidTaxes_8ad6eab9-4262-f34c-4384-fcccebc59d02" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_PrepaidExpenseCurrent" xlink:label="loc_us-gaap_PrepaidExpenseCurrent_d8efa5f9-d69a-54ac-efc6-6e7e03d11640" xlink:type="locator" />
    <calculationArc order="7" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_67f49e84-05fd-4b0b-ae5a-e9913b97cea2" xlink:to="loc_us-gaap_PrepaidExpenseCurrent_d8efa5f9-d69a-54ac-efc6-6e7e03d11640" xlink:type="arc" />
    <loc xlink:href="holx-20150328.xsd#holx_IntercompanyReceivables" xlink:label="loc_holx_IntercompanyReceivables_7d012b5f-8da6-f006-0bc7-9b221b4fc3a3" xlink:type="locator" />
    <calculationArc order="8" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_67f49e84-05fd-4b0b-ae5a-e9913b97cea2" xlink:to="loc_holx_IntercompanyReceivables_7d012b5f-8da6-f006-0bc7-9b221b4fc3a3" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_PropertyPlantAndEquipmentNet" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_a1eaafaf-055b-e423-85ef-4424fb2ef4e4" xlink:type="locator" />
    <calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_b517a4f1-4597-b094-2608-75af660328e1" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_a1eaafaf-055b-e423-85ef-4424fb2ef4e4" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_IntangibleAssetsNetExcludingGoodwill" xlink:label="loc_us-gaap_IntangibleAssetsNetExcludingGoodwill_32ecd7de-6410-d56f-5547-ab1b682ebbc0" xlink:type="locator" />
    <calculationArc order="3" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_b517a4f1-4597-b094-2608-75af660328e1" xlink:to="loc_us-gaap_IntangibleAssetsNetExcludingGoodwill_32ecd7de-6410-d56f-5547-ab1b682ebbc0" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_Goodwill" xlink:label="loc_us-gaap_Goodwill_d1288432-0516-3188-e96b-2bc623375a5a" xlink:type="locator" />
    <calculationArc order="4" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_b517a4f1-4597-b094-2608-75af660328e1" xlink:to="loc_us-gaap_Goodwill_d1288432-0516-3188-e96b-2bc623375a5a" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_OtherAssetsNoncurrent" xlink:label="loc_us-gaap_OtherAssetsNoncurrent_4599c23a-11f0-4277-ea32-9886d26f8792" xlink:type="locator" />
    <calculationArc order="5" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_b517a4f1-4597-b094-2608-75af660328e1" xlink:to="loc_us-gaap_OtherAssetsNoncurrent_4599c23a-11f0-4277-ea32-9886d26f8792" xlink:type="arc" />
    <loc xlink:href="holx-20150328.xsd#holx_IntercompanyNotesReceivable" xlink:label="loc_holx_IntercompanyNotesReceivable_e5f5dcee-a156-0f40-ccac-9b221b1d5593" xlink:type="locator" />
    <calculationArc order="6" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_b517a4f1-4597-b094-2608-75af660328e1" xlink:to="loc_holx_IntercompanyNotesReceivable_e5f5dcee-a156-0f40-ccac-9b221b1d5593" xlink:type="arc" />
    <loc xlink:href="holx-20150328.xsd#holx_InvestmentInSubsidiaries" xlink:label="loc_holx_InvestmentInSubsidiaries_21b2a72f-babd-1ebc-621e-9b22190a1a62" xlink:type="locator" />
    <calculationArc order="7" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_b517a4f1-4597-b094-2608-75af660328e1" xlink:to="loc_holx_InvestmentInSubsidiaries_21b2a72f-babd-1ebc-621e-9b22190a1a62" xlink:type="arc" />
  </calculationLink>
  <calculationLink xlink:role="http://www.hologic.com/role/SupplementalGuarantorCondensedConsolidatingFinancialsScheduleOfSupplementalCondensedConsolidatingStatementOfOperationsDetail" xlink:type="extended">
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_NetIncomeLoss" xlink:label="loc_us-gaap_NetIncomeLoss_faecc408-4bea-6e13-83bd-71b4c2ddf64c" xlink:type="locator" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesMinorityInterestAndIncomeLossFromEquityMethodInvestments" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesMinorityInterestAndIncomeLossFromEquityMethodInvestments_10afc0e9-1faa-0be3-fc32-15d128e46f3f" xlink:type="locator" />
    <calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_faecc408-4bea-6e13-83bd-71b4c2ddf64c" xlink:to="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesMinorityInterestAndIncomeLossFromEquityMethodInvestments_10afc0e9-1faa-0be3-fc32-15d128e46f3f" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_IncomeTaxExpenseBenefit" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_3a83e980-c99b-5657-79fd-300643b7fa11" xlink:type="locator" />
    <calculationArc order="2" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_faecc408-4bea-6e13-83bd-71b4c2ddf64c" xlink:to="loc_us-gaap_IncomeTaxExpenseBenefit_3a83e980-c99b-5657-79fd-300643b7fa11" xlink:type="arc" />
    <loc xlink:href="holx-20150328.xsd#holx_EquityInEarningsLossOfSubsidiaries" xlink:label="loc_holx_EquityInEarningsLossOfSubsidiaries_f14b3dab-dd61-8a2f-2dbb-9b221b593dff" xlink:type="locator" />
    <calculationArc order="3" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_faecc408-4bea-6e13-83bd-71b4c2ddf64c" xlink:to="loc_holx_EquityInEarningsLossOfSubsidiaries_f14b3dab-dd61-8a2f-2dbb-9b221b593dff" xlink:type="arc" />
  </calculationLink>
  <calculationLink xlink:role="http://www.hologic.com/role/SupplementalGuarantorCondensedConsolidatingFinancialsScheduleOfSupplementalCondensedConsolidatingStatementsOfComprehensiveLossIncomeDetail" xlink:type="extended">
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_ComprehensiveIncomeNetOfTax" xlink:label="loc_us-gaap_ComprehensiveIncomeNetOfTax_375e0d17-4818-4239-a370-aada0b6ca8eb" xlink:type="locator" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_NetIncomeLoss" xlink:label="loc_us-gaap_NetIncomeLoss_faecc408-4bea-6e13-83bd-71b4c2ddf64c" xlink:type="locator" />
    <calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_375e0d17-4818-4239-a370-aada0b6ca8eb" xlink:to="loc_us-gaap_NetIncomeLoss_faecc408-4bea-6e13-83bd-71b4c2ddf64c" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_OtherComprehensiveIncomeForeignCurrencyTransactionAndTranslationAdjustmentNetOfTaxPortionAttributableToParent" xlink:label="loc_us-gaap_OtherComprehensiveIncomeForeignCurrencyTransactionAndTranslationAdjustmentNetOfTaxPortionAttributableToParent_f949b2e8-d740-7c2b-d949-74ff04888c67" xlink:type="locator" />
    <calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_375e0d17-4818-4239-a370-aada0b6ca8eb" xlink:to="loc_us-gaap_OtherComprehensiveIncomeForeignCurrencyTransactionAndTranslationAdjustmentNetOfTaxPortionAttributableToParent_f949b2e8-d740-7c2b-d949-74ff04888c67" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_OtherComprehensiveIncomeLossAvailableForSaleSecuritiesAdjustmentNetOfTax" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossAvailableForSaleSecuritiesAdjustmentNetOfTax_9cf69aee-515f-6301-13fd-c65343678f68" xlink:type="locator" />
    <calculationArc order="3" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_375e0d17-4818-4239-a370-aada0b6ca8eb" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossAvailableForSaleSecuritiesAdjustmentNetOfTax_9cf69aee-515f-6301-13fd-c65343678f68" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_OtherComprehensiveIncomeDerivativesQualifyingAsHedgesNetOfTaxPortionAttributableToParent" xlink:label="loc_us-gaap_OtherComprehensiveIncomeDerivativesQualifyingAsHedgesNetOfTaxPortionAttributableToParent_f227397a-ca43-733a-2afb-7868d393b76c" xlink:type="locator" />
    <calculationArc order="4" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_375e0d17-4818-4239-a370-aada0b6ca8eb" xlink:to="loc_us-gaap_OtherComprehensiveIncomeDerivativesQualifyingAsHedgesNetOfTaxPortionAttributableToParent_f227397a-ca43-733a-2afb-7868d393b76c" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_DerivativeInstrumentsGainLossRecognizedInOtherComprehensiveIncomeEffectivePortionNet" xlink:label="loc_us-gaap_DerivativeInstrumentsGainLossRecognizedInOtherComprehensiveIncomeEffectivePortionNet_94c12879-3943-ab4e-516d-23e8930780d9" xlink:type="locator" />
    <calculationArc order="5" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_375e0d17-4818-4239-a370-aada0b6ca8eb" xlink:to="loc_us-gaap_DerivativeInstrumentsGainLossRecognizedInOtherComprehensiveIncomeEffectivePortionNet_94c12879-3943-ab4e-516d-23e8930780d9" xlink:type="arc" />
    <loc xlink:href="http://xbrl.fasb.org/us-gaap/2014/elts/us-gaap-2014-01-31.xsd#us-gaap_OtherComprehensiveIncomeDefinedBenefitPlansAdjustmentNetOfTaxPortionAttributableToParent" xlink:label="loc_us-gaap_OtherComprehensiveIncomeDefinedBenefitPlansAdjustmentNetOfTaxPortionAttributableToParent_e79edc95-de47-7f7d-36d1-71722214678b" xlink:type="locator" />
    <calculationArc order="6" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_375e0d17-4818-4239-a370-aada0b6ca8eb" xlink:to="loc_us-gaap_OtherComprehensiveIncomeDefinedBenefitPlansAdjustmentNetOfTaxPortionAttributableToParent_e79edc95-de47-7f7d-36d1-71722214678b" xlink:type="arc" />
  </calculationLink>
  <calculationLink xlink:role="http://www.hologic.com/role/SupplementalGuarantorCondensedConsolidatingFinancialsTables" xlink:type="extended" />
</linkbase>
