Exhibit 8.1

Subsidiaries |  | Jurisdiction of Incorporation
Ascendis Pharma GmbH |  | Germany
Ascendis Pharma Endocrinology GmbH |  | Germany
Ascendis Pharma, LLC |  | USA
Ascendis Pharma Endocrinology, Inc. |  | USA
Ascendis Pharma, Ophthalmology Division A/S |  | Denmark
Ascendis Pharma Endocrinology Division A/S |  | Denmark
Ascendis Pharma Bone Diseases A/S |  | Denmark
Ascendis Pharma Growth Disorders A/S |  | Denmark
Ascendis Pharma Oncology Division A/S |  | Denmark
Ascendis Pharma Europe A/S |  | Denmark
Ascendis Pharma UK, Limited |  | United Kingdom
Ascendis Pharma Iberia S.L. |  | Spain
Ascendis Pharma France SASU |  | France
Ascendis Pharma Italia S.R.L. |  | Italy
Ascendis Pharma Sverige AB |  | Sweden
Ascendis Pharma Switzerland GmbH |  | Switzerland
Ascendis Pharma Belgium BV |  | Belgium
ASND Portugal, Unipessoal, Lda. |  | Portugal