<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with Wdesk from Workiva-->
<!--Copyright 2023 Workiva-->
<!--r:d65d7f3e-3d62-4ca4-a55c-5737ce9c2410,g:2d4b4122-ada3-43b6-9335-248bbd91fb59-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/ConsolidatedBalanceSheets" xlink:type="simple" xlink:href="efsc-20221231.xsd#ConsolidatedBalanceSheets"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/ConsolidatedBalanceSheets" xlink:type="extended" id="i0e93110163714bb7a33b6ae361dba589_ConsolidatedBalanceSheets">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_40e43280-c000-4fb4-aa5c-cb561e9152f3" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsAbstract_2ac6ff07-9032-449e-a1a0-9cc845ff4c04" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AssetsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_40e43280-c000-4fb4-aa5c-cb561e9152f3" xlink:to="loc_us-gaap_AssetsAbstract_2ac6ff07-9032-449e-a1a0-9cc845ff4c04" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndDueFromBanks_47d54421-a772-43ad-9b35-e84a31ec2576" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CashAndDueFromBanks"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_2ac6ff07-9032-449e-a1a0-9cc845ff4c04" xlink:to="loc_us-gaap_CashAndDueFromBanks_47d54421-a772-43ad-9b35-e84a31ec2576" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FederalFundsSold_85493169-e0f5-4230-98b2-3f688c61054f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FederalFundsSold"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_2ac6ff07-9032-449e-a1a0-9cc845ff4c04" xlink:to="loc_us-gaap_FederalFundsSold_85493169-e0f5-4230-98b2-3f688c61054f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestBearingDepositsInBanks_a5b23be0-3d7f-4847-bcd2-748ae4780a0b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestBearingDepositsInBanks"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_2ac6ff07-9032-449e-a1a0-9cc845ff4c04" xlink:to="loc_us-gaap_InterestBearingDepositsInBanks_a5b23be0-3d7f-4847-bcd2-748ae4780a0b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsAndFederalFundsSold_f693b026-e313-466d-ab42-426e342b9c6f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CashCashEquivalentsAndFederalFundsSold"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_2ac6ff07-9032-449e-a1a0-9cc845ff4c04" xlink:to="loc_us-gaap_CashCashEquivalentsAndFederalFundsSold_f693b026-e313-466d-ab42-426e342b9c6f" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_InterestBearingDepositsGreaterThan90Days_d5fd550c-e401-4622-ae34-6012321d57f1" xlink:href="efsc-20221231.xsd#efsc_InterestBearingDepositsGreaterThan90Days"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_2ac6ff07-9032-449e-a1a0-9cc845ff4c04" xlink:to="loc_efsc_InterestBearingDepositsGreaterThan90Days_d5fd550c-e401-4622-ae34-6012321d57f1" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_cd82e078-7bf2-43a5-8344-88a2a68ef21c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AvailableForSaleSecuritiesDebtSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_2ac6ff07-9032-449e-a1a0-9cc845ff4c04" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_cd82e078-7bf2-43a5-8344-88a2a68ef21c" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_DebtSecuritiesHeldToMaturityNetOfAllowanceForCreditLosses_45f6397f-2cbf-44fe-9fae-be43e8979fe4" xlink:href="efsc-20221231.xsd#efsc_DebtSecuritiesHeldToMaturityNetOfAllowanceForCreditLosses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_2ac6ff07-9032-449e-a1a0-9cc845ff4c04" xlink:to="loc_efsc_DebtSecuritiesHeldToMaturityNetOfAllowanceForCreditLosses_45f6397f-2cbf-44fe-9fae-be43e8979fe4" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TradeAndLoansReceivablesHeldForSaleNetNotPartOfDisposalGroup_0778ca0f-8505-4dce-924c-b058d7470970" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TradeAndLoansReceivablesHeldForSaleNetNotPartOfDisposalGroup"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_2ac6ff07-9032-449e-a1a0-9cc845ff4c04" xlink:to="loc_us-gaap_TradeAndLoansReceivablesHeldForSaleNetNotPartOfDisposalGroup_0778ca0f-8505-4dce-924c-b058d7470970" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LoansAndLeasesReceivableGrossCarryingAmount_0a333a79-7dff-44f4-a82c-40b147e6a7b6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LoansAndLeasesReceivableGrossCarryingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_2ac6ff07-9032-449e-a1a0-9cc845ff4c04" xlink:to="loc_us-gaap_LoansAndLeasesReceivableGrossCarryingAmount_0a333a79-7dff-44f4-a82c-40b147e6a7b6" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LoansAndLeasesReceivableAllowance_8a697a77-f472-47d0-a6f2-1d7c1618d50f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LoansAndLeasesReceivableAllowance"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_2ac6ff07-9032-449e-a1a0-9cc845ff4c04" xlink:to="loc_us-gaap_LoansAndLeasesReceivableAllowance_8a697a77-f472-47d0-a6f2-1d7c1618d50f" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LoansAndLeasesReceivableNetReportedAmount_d87ad242-f232-4d3c-8275-9b2508378399" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LoansAndLeasesReceivableNetReportedAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_2ac6ff07-9032-449e-a1a0-9cc845ff4c04" xlink:to="loc_us-gaap_LoansAndLeasesReceivableNetReportedAmount_d87ad242-f232-4d3c-8275-9b2508378399" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherInvestmentsAndSecuritiesAtCost_179f89c9-c21d-47ce-908c-69c768c45bfa" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherInvestmentsAndSecuritiesAtCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_2ac6ff07-9032-449e-a1a0-9cc845ff4c04" xlink:to="loc_us-gaap_OtherInvestmentsAndSecuritiesAtCost_179f89c9-c21d-47ce-908c-69c768c45bfa" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_508c9e5f-c706-4137-b602-bafc2cbb62b5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_2ac6ff07-9032-449e-a1a0-9cc845ff4c04" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_508c9e5f-c706-4137-b602-bafc2cbb62b5" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Goodwill_24d6bfaf-c646-43e8-9fab-f5e3abf05b48" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_Goodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_2ac6ff07-9032-449e-a1a0-9cc845ff4c04" xlink:to="loc_us-gaap_Goodwill_24d6bfaf-c646-43e8-9fab-f5e3abf05b48" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IntangibleAssetsNetExcludingGoodwill_93bb0cb4-bfab-42c0-bab8-b96cad40314c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IntangibleAssetsNetExcludingGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_2ac6ff07-9032-449e-a1a0-9cc845ff4c04" xlink:to="loc_us-gaap_IntangibleAssetsNetExcludingGoodwill_93bb0cb4-bfab-42c0-bab8-b96cad40314c" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrepaidExpenseAndOtherAssets_31705daf-a7ef-4143-b83c-46ee15216de2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PrepaidExpenseAndOtherAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_2ac6ff07-9032-449e-a1a0-9cc845ff4c04" xlink:to="loc_us-gaap_PrepaidExpenseAndOtherAssets_31705daf-a7ef-4143-b83c-46ee15216de2" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Assets_b9979e67-94ee-48f7-bbc8-9fca7277ae68" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_Assets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_2ac6ff07-9032-449e-a1a0-9cc845ff4c04" xlink:to="loc_us-gaap_Assets_b9979e67-94ee-48f7-bbc8-9fca7277ae68" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_db1ebf1d-b2bf-441b-991b-1aa97531df8c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LiabilitiesAndStockholdersEquityAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_40e43280-c000-4fb4-aa5c-cb561e9152f3" xlink:to="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_db1ebf1d-b2bf-441b-991b-1aa97531df8c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NoninterestBearingDomesticDepositDemand_2e55a0c5-441e-47e6-b2a0-a24c42362be6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NoninterestBearingDomesticDepositDemand"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_db1ebf1d-b2bf-441b-991b-1aa97531df8c" xlink:to="loc_us-gaap_NoninterestBearingDomesticDepositDemand_2e55a0c5-441e-47e6-b2a0-a24c42362be6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestBearingDomesticDepositChecking_3edc8dc6-d9d3-4d28-89cb-f783c56d688b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestBearingDomesticDepositChecking"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_db1ebf1d-b2bf-441b-991b-1aa97531df8c" xlink:to="loc_us-gaap_InterestBearingDomesticDepositChecking_3edc8dc6-d9d3-4d28-89cb-f783c56d688b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepositsMoneyMarketDeposits_a3b9e6c3-61b6-4381-9886-4fb455e8de6f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DepositsMoneyMarketDeposits"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_db1ebf1d-b2bf-441b-991b-1aa97531df8c" xlink:to="loc_us-gaap_DepositsMoneyMarketDeposits_a3b9e6c3-61b6-4381-9886-4fb455e8de6f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepositsSavingsDeposits_4e1a9a5c-19d3-464d-83e9-86719b74d1ca" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DepositsSavingsDeposits"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_db1ebf1d-b2bf-441b-991b-1aa97531df8c" xlink:to="loc_us-gaap_DepositsSavingsDeposits_4e1a9a5c-19d3-464d-83e9-86719b74d1ca" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TimeDepositsAbstract_8307361b-a223-4dd9-9956-6b2df15d3455" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TimeDepositsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_db1ebf1d-b2bf-441b-991b-1aa97531df8c" xlink:to="loc_us-gaap_TimeDepositsAbstract_8307361b-a223-4dd9-9956-6b2df15d3455" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestBearingDomesticDepositBrokered_321173d4-b707-4001-8d7b-4f1867d55ab4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestBearingDomesticDepositBrokered"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TimeDepositsAbstract_8307361b-a223-4dd9-9956-6b2df15d3455" xlink:to="loc_us-gaap_InterestBearingDomesticDepositBrokered_321173d4-b707-4001-8d7b-4f1867d55ab4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestBearingDomesticDepositOtherTimeDeposit_c3b46c28-5147-4253-beb9-29556354f8f5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestBearingDomesticDepositOtherTimeDeposit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TimeDepositsAbstract_8307361b-a223-4dd9-9956-6b2df15d3455" xlink:to="loc_us-gaap_InterestBearingDomesticDepositOtherTimeDeposit_c3b46c28-5147-4253-beb9-29556354f8f5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Deposits_252aeae7-584e-4a27-819f-e8fcce36fb14" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_Deposits"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_db1ebf1d-b2bf-441b-991b-1aa97531df8c" xlink:to="loc_us-gaap_Deposits_252aeae7-584e-4a27-819f-e8fcce36fb14" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubordinatedDebt_0cf9e736-f18c-4988-abbd-ee33dc34239a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SubordinatedDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_db1ebf1d-b2bf-441b-991b-1aa97531df8c" xlink:to="loc_us-gaap_SubordinatedDebt_0cf9e736-f18c-4988-abbd-ee33dc34239a" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FederalHomeLoanBankAdvancesLongTerm_d8719823-de51-4593-aa1b-6d43c0396e5f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FederalHomeLoanBankAdvancesLongTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_db1ebf1d-b2bf-441b-991b-1aa97531df8c" xlink:to="loc_us-gaap_FederalHomeLoanBankAdvancesLongTerm_d8719823-de51-4593-aa1b-6d43c0396e5f" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherBorrowings_7a4e5827-5aff-4d55-b1b6-7b4c6ea30fb9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherBorrowings"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_db1ebf1d-b2bf-441b-991b-1aa97531df8c" xlink:to="loc_us-gaap_OtherBorrowings_7a4e5827-5aff-4d55-b1b6-7b4c6ea30fb9" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilities_9aeb739e-3757-4cc0-babe-1b25fd5aaa94" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_db1ebf1d-b2bf-441b-991b-1aa97531df8c" xlink:to="loc_us-gaap_OtherLiabilities_9aeb739e-3757-4cc0-babe-1b25fd5aaa94" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_5e93dbc4-4356-4711-a74a-951eba117980" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_Liabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_db1ebf1d-b2bf-441b-991b-1aa97531df8c" xlink:to="loc_us-gaap_Liabilities_5e93dbc4-4356-4711-a74a-951eba117980" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityAbstract_9584ccf4-223d-48ec-a1d1-8d5bfc496914" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockholdersEquityAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_db1ebf1d-b2bf-441b-991b-1aa97531df8c" xlink:to="loc_us-gaap_StockholdersEquityAbstract_9584ccf4-223d-48ec-a1d1-8d5bfc496914" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockValue_85f4051b-0f98-44cc-b4ff-49f0781f96e6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PreferredStockValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_9584ccf4-223d-48ec-a1d1-8d5bfc496914" xlink:to="loc_us-gaap_PreferredStockValue_85f4051b-0f98-44cc-b4ff-49f0781f96e6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockValue_65d6b811-69dd-43dc-9b73-88c9e50fd5d9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommonStockValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_9584ccf4-223d-48ec-a1d1-8d5bfc496914" xlink:to="loc_us-gaap_CommonStockValue_65d6b811-69dd-43dc-9b73-88c9e50fd5d9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockValue_aebf95e0-87d4-4317-9933-ff9d355f824b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TreasuryStockValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_9584ccf4-223d-48ec-a1d1-8d5bfc496914" xlink:to="loc_us-gaap_TreasuryStockValue_aebf95e0-87d4-4317-9933-ff9d355f824b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapital_4ed1e22b-2686-4a6a-a470-d231504d3106" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AdditionalPaidInCapital"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_9584ccf4-223d-48ec-a1d1-8d5bfc496914" xlink:to="loc_us-gaap_AdditionalPaidInCapital_4ed1e22b-2686-4a6a-a470-d231504d3106" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsAccumulatedDeficit_613ed1ec-fac5-4dfd-934e-fb1247312ba9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RetainedEarningsAccumulatedDeficit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_9584ccf4-223d-48ec-a1d1-8d5bfc496914" xlink:to="loc_us-gaap_RetainedEarningsAccumulatedDeficit_613ed1ec-fac5-4dfd-934e-fb1247312ba9" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_c47d6022-783f-459a-95fa-2c8a512d226e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_9584ccf4-223d-48ec-a1d1-8d5bfc496914" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_c47d6022-783f-459a-95fa-2c8a512d226e" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_a4ac4fc4-edfd-4b88-a9e5-5aa8ffd4a1ca" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_9584ccf4-223d-48ec-a1d1-8d5bfc496914" xlink:to="loc_us-gaap_StockholdersEquity_a4ac4fc4-edfd-4b88-a9e5-5aa8ffd4a1ca" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquity_a57b601d-9d60-45ec-99fb-8c21ceaec43f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LiabilitiesAndStockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_db1ebf1d-b2bf-441b-991b-1aa97531df8c" xlink:to="loc_us-gaap_LiabilitiesAndStockholdersEquity_a57b601d-9d60-45ec-99fb-8c21ceaec43f" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_76dbff2f-c7f9-4bb2-9452-f8ef326295a2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_40e43280-c000-4fb4-aa5c-cb561e9152f3" xlink:to="loc_us-gaap_StatementTable_76dbff2f-c7f9-4bb2-9452-f8ef326295a2" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementScenarioAxis_bfe78377-b6d7-4d94-8fc8-913c4fb003d3" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_StatementScenarioAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_76dbff2f-c7f9-4bb2-9452-f8ef326295a2" xlink:to="loc_srt_StatementScenarioAxis_bfe78377-b6d7-4d94-8fc8-913c4fb003d3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_bfe78377-b6d7-4d94-8fc8-913c4fb003d3_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementScenarioAxis_bfe78377-b6d7-4d94-8fc8-913c4fb003d3" xlink:to="loc_srt_ScenarioUnspecifiedDomain_bfe78377-b6d7-4d94-8fc8-913c4fb003d3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_a6a683ee-fb85-4557-94e9-cab240a6c7dd" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementScenarioAxis_bfe78377-b6d7-4d94-8fc8-913c4fb003d3" xlink:to="loc_srt_ScenarioUnspecifiedDomain_a6a683ee-fb85-4557-94e9-cab240a6c7dd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_5331a681-8376-4e4a-8bde-3b43d6c1a11b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_76dbff2f-c7f9-4bb2-9452-f8ef326295a2" xlink:to="loc_us-gaap_StatementClassOfStockAxis_5331a681-8376-4e4a-8bde-3b43d6c1a11b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_5331a681-8376-4e4a-8bde-3b43d6c1a11b_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_5331a681-8376-4e4a-8bde-3b43d6c1a11b" xlink:to="loc_us-gaap_ClassOfStockDomain_5331a681-8376-4e4a-8bde-3b43d6c1a11b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_bed9c6ef-8b6f-4ada-b953-63afb3e90a7c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_5331a681-8376-4e4a-8bde-3b43d6c1a11b" xlink:to="loc_us-gaap_ClassOfStockDomain_bed9c6ef-8b6f-4ada-b953-63afb3e90a7c" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/ConsolidatedBalanceSheetsParenthetical" xlink:type="simple" xlink:href="efsc-20221231.xsd#ConsolidatedBalanceSheetsParenthetical"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/ConsolidatedBalanceSheetsParenthetical" xlink:type="extended" id="iaf13c9470cc043de823932f37b51b321_ConsolidatedBalanceSheetsParenthetical">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_e640c8ac-592f-4a97-8a71-9e86942bbed3" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsAbstract_b9d8a30f-2c9c-4cb1-8255-ba5f7ed11b9a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AssetsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e640c8ac-592f-4a97-8a71-9e86942bbed3" xlink:to="loc_us-gaap_AssetsAbstract_b9d8a30f-2c9c-4cb1-8255-ba5f7ed11b9a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CollateralPledged_96822da4-b661-4c27-9108-f7081cd17907" xlink:href="efsc-20221231.xsd#efsc_CollateralPledged"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_b9d8a30f-2c9c-4cb1-8255-ba5f7ed11b9a" xlink:to="loc_efsc_CollateralPledged_96822da4-b661-4c27-9108-f7081cd17907" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityAbstract_c6754cd8-bd0d-4b04-a725-542e2cc8770f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockholdersEquityAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_e640c8ac-592f-4a97-8a71-9e86942bbed3" xlink:to="loc_us-gaap_StockholdersEquityAbstract_c6754cd8-bd0d-4b04-a725-542e2cc8770f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockParOrStatedValuePerShare_4707661d-8603-4830-bac8-abc41fb50239" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PreferredStockParOrStatedValuePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_c6754cd8-bd0d-4b04-a725-542e2cc8770f" xlink:to="loc_us-gaap_PreferredStockParOrStatedValuePerShare_4707661d-8603-4830-bac8-abc41fb50239" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesAuthorized_99e61102-7896-47d2-93ff-2b7e51aa6918" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PreferredStockSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_c6754cd8-bd0d-4b04-a725-542e2cc8770f" xlink:to="loc_us-gaap_PreferredStockSharesAuthorized_99e61102-7896-47d2-93ff-2b7e51aa6918" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesIssued_022ce058-5f8e-4413-ba5a-8959b1deaf7c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PreferredStockSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_c6754cd8-bd0d-4b04-a725-542e2cc8770f" xlink:to="loc_us-gaap_PreferredStockSharesIssued_022ce058-5f8e-4413-ba5a-8959b1deaf7c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesOutstanding_79bae155-5925-42a8-8213-8f1d840d6ea8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PreferredStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_c6754cd8-bd0d-4b04-a725-542e2cc8770f" xlink:to="loc_us-gaap_PreferredStockSharesOutstanding_79bae155-5925-42a8-8213-8f1d840d6ea8" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockLiquidationPreference_a54a740d-6fe6-4a38-a816-e26a72506a1d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PreferredStockLiquidationPreference"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_c6754cd8-bd0d-4b04-a725-542e2cc8770f" xlink:to="loc_us-gaap_PreferredStockLiquidationPreference_a54a740d-6fe6-4a38-a816-e26a72506a1d" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockParOrStatedValuePerShare_79ff8b07-9e5d-483a-a113-b0a9793e6783" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommonStockParOrStatedValuePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_c6754cd8-bd0d-4b04-a725-542e2cc8770f" xlink:to="loc_us-gaap_CommonStockParOrStatedValuePerShare_79ff8b07-9e5d-483a-a113-b0a9793e6783" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesAuthorized_8bfd9140-df37-4be6-aabc-6e6863cc8298" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommonStockSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_c6754cd8-bd0d-4b04-a725-542e2cc8770f" xlink:to="loc_us-gaap_CommonStockSharesAuthorized_8bfd9140-df37-4be6-aabc-6e6863cc8298" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesIssued_e88c28fd-bf01-4083-a76e-feef99b85c84" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommonStockSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_c6754cd8-bd0d-4b04-a725-542e2cc8770f" xlink:to="loc_us-gaap_CommonStockSharesIssued_e88c28fd-bf01-4083-a76e-feef99b85c84" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_7f3f6f61-6f3d-4ef4-b72f-3e961563e2d5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_c6754cd8-bd0d-4b04-a725-542e2cc8770f" xlink:to="loc_us-gaap_CommonStockSharesOutstanding_7f3f6f61-6f3d-4ef4-b72f-3e961563e2d5" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockShares_6d9439b4-f503-4bf5-bb2b-a166306536cb" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TreasuryStockShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_c6754cd8-bd0d-4b04-a725-542e2cc8770f" xlink:to="loc_us-gaap_TreasuryStockShares_6d9439b4-f503-4bf5-bb2b-a166306536cb" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_93879f76-41b8-4c1b-9072-1acca39063a6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_e640c8ac-592f-4a97-8a71-9e86942bbed3" xlink:to="loc_us-gaap_StatementTable_93879f76-41b8-4c1b-9072-1acca39063a6" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementScenarioAxis_95a914fe-9309-4b47-871b-0da5e083f81a" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_StatementScenarioAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_93879f76-41b8-4c1b-9072-1acca39063a6" xlink:to="loc_srt_StatementScenarioAxis_95a914fe-9309-4b47-871b-0da5e083f81a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_95a914fe-9309-4b47-871b-0da5e083f81a_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementScenarioAxis_95a914fe-9309-4b47-871b-0da5e083f81a" xlink:to="loc_srt_ScenarioUnspecifiedDomain_95a914fe-9309-4b47-871b-0da5e083f81a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_03ea8db7-9530-4c6a-8d07-36c237cf6c73" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementScenarioAxis_95a914fe-9309-4b47-871b-0da5e083f81a" xlink:to="loc_srt_ScenarioUnspecifiedDomain_03ea8db7-9530-4c6a-8d07-36c237cf6c73" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_e548d419-e47c-4308-a81d-864316397883" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_93879f76-41b8-4c1b-9072-1acca39063a6" xlink:to="loc_us-gaap_StatementClassOfStockAxis_e548d419-e47c-4308-a81d-864316397883" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_e548d419-e47c-4308-a81d-864316397883_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_e548d419-e47c-4308-a81d-864316397883" xlink:to="loc_us-gaap_ClassOfStockDomain_e548d419-e47c-4308-a81d-864316397883_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_977a02b5-e9af-4fe7-aa1b-374a3e0c7de4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_e548d419-e47c-4308-a81d-864316397883" xlink:to="loc_us-gaap_ClassOfStockDomain_977a02b5-e9af-4fe7-aa1b-374a3e0c7de4" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/ConsolidatedStatementsofOperations" xlink:type="simple" xlink:href="efsc-20221231.xsd#ConsolidatedStatementsofOperations"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/ConsolidatedStatementsofOperations" xlink:type="extended" id="i2ed0464810a84ea3951158154cdd1713_ConsolidatedStatementsofOperations">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_79276b1f-9c5b-4cb4-a82f-55dc6a8c83e4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestAndDividendIncomeOperatingAbstract_fa254308-9482-4460-b0e5-0f2fb0b5b10a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestAndDividendIncomeOperatingAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_79276b1f-9c5b-4cb4-a82f-55dc6a8c83e4" xlink:to="loc_us-gaap_InterestAndDividendIncomeOperatingAbstract_fa254308-9482-4460-b0e5-0f2fb0b5b10a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestAndFeeIncomeLoansAndLeases_aa2cbef0-db6c-4361-a98e-e8fb016ad049" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestAndFeeIncomeLoansAndLeases"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterestAndDividendIncomeOperatingAbstract_fa254308-9482-4460-b0e5-0f2fb0b5b10a" xlink:to="loc_us-gaap_InterestAndFeeIncomeLoansAndLeases_aa2cbef0-db6c-4361-a98e-e8fb016ad049" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestAndDividendIncomeSecuritiesByTaxableStatusAbstract_9dd5d884-0dd7-454f-814a-f854d17ad2fc" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestAndDividendIncomeSecuritiesByTaxableStatusAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterestAndDividendIncomeOperatingAbstract_fa254308-9482-4460-b0e5-0f2fb0b5b10a" xlink:to="loc_us-gaap_InterestAndDividendIncomeSecuritiesByTaxableStatusAbstract_9dd5d884-0dd7-454f-814a-f854d17ad2fc" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestIncomeSecuritiesTaxable_61e28677-f14c-4af7-a071-b2e8f04180a8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestIncomeSecuritiesTaxable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterestAndDividendIncomeSecuritiesByTaxableStatusAbstract_9dd5d884-0dd7-454f-814a-f854d17ad2fc" xlink:to="loc_us-gaap_InterestIncomeSecuritiesTaxable_61e28677-f14c-4af7-a071-b2e8f04180a8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestIncomeSecuritiesTaxExempt_e96e2598-2c69-4254-bc61-8092df11a896" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestIncomeSecuritiesTaxExempt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterestAndDividendIncomeSecuritiesByTaxableStatusAbstract_9dd5d884-0dd7-454f-814a-f854d17ad2fc" xlink:to="loc_us-gaap_InterestIncomeSecuritiesTaxExempt_e96e2598-2c69-4254-bc61-8092df11a896" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestIncomeDomesticDeposits_7a1c3ed8-264f-40c1-9652-33cc70db0dae" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestIncomeDomesticDeposits"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterestAndDividendIncomeOperatingAbstract_fa254308-9482-4460-b0e5-0f2fb0b5b10a" xlink:to="loc_us-gaap_InterestIncomeDomesticDeposits_7a1c3ed8-264f-40c1-9652-33cc70db0dae" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DividendIncomeOperating_0b910ff9-7537-4ff4-bacb-b6b163298b74" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DividendIncomeOperating"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterestAndDividendIncomeOperatingAbstract_fa254308-9482-4460-b0e5-0f2fb0b5b10a" xlink:to="loc_us-gaap_DividendIncomeOperating_0b910ff9-7537-4ff4-bacb-b6b163298b74" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestAndDividendIncomeOperating_29ca7907-98c2-46a9-959c-87d5bf0a1c1f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestAndDividendIncomeOperating"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterestAndDividendIncomeOperatingAbstract_fa254308-9482-4460-b0e5-0f2fb0b5b10a" xlink:to="loc_us-gaap_InterestAndDividendIncomeOperating_29ca7907-98c2-46a9-959c-87d5bf0a1c1f" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseAbstract_8d7b4778-d276-488b-8dfc-2d6345a82935" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestExpenseAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_79276b1f-9c5b-4cb4-a82f-55dc6a8c83e4" xlink:to="loc_us-gaap_InterestExpenseAbstract_8d7b4778-d276-488b-8dfc-2d6345a82935" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseDeposits_193b3803-7fdd-497d-85be-56978c014ffb" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestExpenseDeposits"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterestExpenseAbstract_8d7b4778-d276-488b-8dfc-2d6345a82935" xlink:to="loc_us-gaap_InterestExpenseDeposits_193b3803-7fdd-497d-85be-56978c014ffb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseSubordinatedNotesAndDebentures_d38f2b7c-ac35-43a9-8b73-015d6d02c648" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestExpenseSubordinatedNotesAndDebentures"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterestExpenseAbstract_8d7b4778-d276-488b-8dfc-2d6345a82935" xlink:to="loc_us-gaap_InterestExpenseSubordinatedNotesAndDebentures_d38f2b7c-ac35-43a9-8b73-015d6d02c648" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseFederalHomeLoanBankAndFederalReserveBankAdvancesLongTerm_21151d29-28be-4a85-9840-6abb89198a19" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestExpenseFederalHomeLoanBankAndFederalReserveBankAdvancesLongTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterestExpenseAbstract_8d7b4778-d276-488b-8dfc-2d6345a82935" xlink:to="loc_us-gaap_InterestExpenseFederalHomeLoanBankAndFederalReserveBankAdvancesLongTerm_21151d29-28be-4a85-9840-6abb89198a19" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseFederalFundsPurchasedAndSecuritiesSoldUnderAgreementsToRepurchase_e24dec1e-8ef4-4405-bf76-51d06ad2b672" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestExpenseFederalFundsPurchasedAndSecuritiesSoldUnderAgreementsToRepurchase"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterestExpenseAbstract_8d7b4778-d276-488b-8dfc-2d6345a82935" xlink:to="loc_us-gaap_InterestExpenseFederalFundsPurchasedAndSecuritiesSoldUnderAgreementsToRepurchase_e24dec1e-8ef4-4405-bf76-51d06ad2b672" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpense_04ff4b92-8ebb-441a-9801-3212370c0f76" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterestExpenseAbstract_8d7b4778-d276-488b-8dfc-2d6345a82935" xlink:to="loc_us-gaap_InterestExpense_04ff4b92-8ebb-441a-9801-3212370c0f76" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestIncomeExpenseNet_c87000de-592c-4bf3-9178-82aec9e29bdb" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestIncomeExpenseNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_79276b1f-9c5b-4cb4-a82f-55dc6a8c83e4" xlink:to="loc_us-gaap_InterestIncomeExpenseNet_c87000de-592c-4bf3-9178-82aec9e29bdb" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ProvisionForLoanLossesNotCoveredUnderFdicLossShare_c6822a29-782f-489d-bb32-476215c6e9ac" xlink:href="efsc-20221231.xsd#efsc_ProvisionForLoanLossesNotCoveredUnderFdicLossShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_79276b1f-9c5b-4cb4-a82f-55dc6a8c83e4" xlink:to="loc_efsc_ProvisionForLoanLossesNotCoveredUnderFdicLossShare_c6822a29-782f-489d-bb32-476215c6e9ac" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestIncomeExpenseAfterProvisionForLoanLoss_e86dcabe-9f10-48fa-bb5c-78acb00bf81e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestIncomeExpenseAfterProvisionForLoanLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_79276b1f-9c5b-4cb4-a82f-55dc6a8c83e4" xlink:to="loc_us-gaap_InterestIncomeExpenseAfterProvisionForLoanLoss_e86dcabe-9f10-48fa-bb5c-78acb00bf81e" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NoninterestIncomeAbstract_0bb7f0e7-0023-44db-8dd7-ff1a6616c42b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NoninterestIncomeAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_79276b1f-9c5b-4cb4-a82f-55dc6a8c83e4" xlink:to="loc_us-gaap_NoninterestIncomeAbstract_0bb7f0e7-0023-44db-8dd7-ff1a6616c42b" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NoninterestIncome_1405efb0-a84f-4dd4-a92d-c3652af211d0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NoninterestIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoninterestIncomeAbstract_0bb7f0e7-0023-44db-8dd7-ff1a6616c42b" xlink:to="loc_us-gaap_NoninterestIncome_1405efb0-a84f-4dd4-a92d-c3652af211d0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NoninterestExpenseAbstract_9a14b04a-8fba-43f6-ba75-bc068182957e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NoninterestExpenseAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_79276b1f-9c5b-4cb4-a82f-55dc6a8c83e4" xlink:to="loc_us-gaap_NoninterestExpenseAbstract_9a14b04a-8fba-43f6-ba75-bc068182957e" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LaborAndRelatedExpense_d1b19214-0274-45f3-96ee-0c559e66f987" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LaborAndRelatedExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoninterestExpenseAbstract_9a14b04a-8fba-43f6-ba75-bc068182957e" xlink:to="loc_us-gaap_LaborAndRelatedExpense_d1b19214-0274-45f3-96ee-0c559e66f987" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OccupancyNet_677e6dbb-b31e-4565-8632-6b11cf91f668" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OccupancyNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoninterestExpenseAbstract_9a14b04a-8fba-43f6-ba75-bc068182957e" xlink:to="loc_us-gaap_OccupancyNet_677e6dbb-b31e-4565-8632-6b11cf91f668" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InformationTechnologyAndDataProcessing_d3bbf8dd-b74c-4e31-9160-675a1bd2fa24" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InformationTechnologyAndDataProcessing"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoninterestExpenseAbstract_9a14b04a-8fba-43f6-ba75-bc068182957e" xlink:to="loc_us-gaap_InformationTechnologyAndDataProcessing_d3bbf8dd-b74c-4e31-9160-675a1bd2fa24" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProfessionalFees_6c00c85d-c4fb-4db9-8a09-532f9f6d9a2b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ProfessionalFees"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoninterestExpenseAbstract_9a14b04a-8fba-43f6-ba75-bc068182957e" xlink:to="loc_us-gaap_ProfessionalFees_6c00c85d-c4fb-4db9-8a09-532f9f6d9a2b" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_BranchClosureExpenses_01649dde-d7c5-4b79-a40b-398a649ad798" xlink:href="efsc-20221231.xsd#efsc_BranchClosureExpenses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoninterestExpenseAbstract_9a14b04a-8fba-43f6-ba75-bc068182957e" xlink:to="loc_efsc_BranchClosureExpenses_01649dde-d7c5-4b79-a40b-398a649ad798" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_MergerRelatedExpenses_c9b8a8c6-bad7-42ae-a4ec-357d0a1a1ef1" xlink:href="efsc-20221231.xsd#efsc_MergerRelatedExpenses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoninterestExpenseAbstract_9a14b04a-8fba-43f6-ba75-bc068182957e" xlink:to="loc_efsc_MergerRelatedExpenses_c9b8a8c6-bad7-42ae-a4ec-357d0a1a1ef1" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNoninterestExpense_57d73838-fbdc-48f3-8511-86a113ab7aa2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherNoninterestExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoninterestExpenseAbstract_9a14b04a-8fba-43f6-ba75-bc068182957e" xlink:to="loc_us-gaap_OtherNoninterestExpense_57d73838-fbdc-48f3-8511-86a113ab7aa2" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NoninterestExpense_4495f39c-d6fa-499c-bbfa-645c0203891d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NoninterestExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoninterestExpenseAbstract_9a14b04a-8fba-43f6-ba75-bc068182957e" xlink:to="loc_us-gaap_NoninterestExpense_4495f39c-d6fa-499c-bbfa-645c0203891d" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesMinorityInterestAndIncomeLossFromEquityMethodInvestments_6a8b4859-aa28-4971-889c-1661c7a9155f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesMinorityInterestAndIncomeLossFromEquityMethodInvestments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_79276b1f-9c5b-4cb4-a82f-55dc6a8c83e4" xlink:to="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesMinorityInterestAndIncomeLossFromEquityMethodInvestments_6a8b4859-aa28-4971-889c-1661c7a9155f" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_70948f04-5a0f-4381-a31a-2dcb916c1c85" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeTaxExpenseBenefit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_79276b1f-9c5b-4cb4-a82f-55dc6a8c83e4" xlink:to="loc_us-gaap_IncomeTaxExpenseBenefit_70948f04-5a0f-4381-a31a-2dcb916c1c85" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_f95628ad-4248-4959-bb69-b25d4cb3087f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_79276b1f-9c5b-4cb4-a82f-55dc6a8c83e4" xlink:to="loc_us-gaap_NetIncomeLoss_f95628ad-4248-4959-bb69-b25d4cb3087f" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockDividendsAndOtherAdjustments_7ae5d3b6-a543-42bd-b97f-73e01d73ec17" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PreferredStockDividendsAndOtherAdjustments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_79276b1f-9c5b-4cb4-a82f-55dc6a8c83e4" xlink:to="loc_us-gaap_PreferredStockDividendsAndOtherAdjustments_7ae5d3b6-a543-42bd-b97f-73e01d73ec17" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic_ac0326ff-01fd-4086-bab8-0b2633d424eb" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_79276b1f-9c5b-4cb4-a82f-55dc6a8c83e4" xlink:to="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic_ac0326ff-01fd-4086-bab8-0b2633d424eb" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareBasicAndDilutedAbstract_a2f2f1ea-e83c-4a36-a586-7836dc3ea6ad" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EarningsPerShareBasicAndDilutedAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_79276b1f-9c5b-4cb4-a82f-55dc6a8c83e4" xlink:to="loc_us-gaap_EarningsPerShareBasicAndDilutedAbstract_a2f2f1ea-e83c-4a36-a586-7836dc3ea6ad" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareBasic_eeb5e4ad-98e4-4fa1-ae09-cb9b42c9b7b7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EarningsPerShareBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EarningsPerShareBasicAndDilutedAbstract_a2f2f1ea-e83c-4a36-a586-7836dc3ea6ad" xlink:to="loc_us-gaap_EarningsPerShareBasic_eeb5e4ad-98e4-4fa1-ae09-cb9b42c9b7b7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareDiluted_e71fa318-ed96-4f3f-bde1-ad39ae36c610" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EarningsPerShareDiluted"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EarningsPerShareBasicAndDilutedAbstract_a2f2f1ea-e83c-4a36-a586-7836dc3ea6ad" xlink:to="loc_us-gaap_EarningsPerShareDiluted_e71fa318-ed96-4f3f-bde1-ad39ae36c610" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_a360c443-8dce-4606-913a-68ecf38514ff" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_79276b1f-9c5b-4cb4-a82f-55dc6a8c83e4" xlink:to="loc_us-gaap_StatementTable_a360c443-8dce-4606-913a-68ecf38514ff" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_63e965fe-57f5-4a8d-b2bc-64088609b7d2" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_a360c443-8dce-4606-913a-68ecf38514ff" xlink:to="loc_srt_ProductOrServiceAxis_63e965fe-57f5-4a8d-b2bc-64088609b7d2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_63e965fe-57f5-4a8d-b2bc-64088609b7d2_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_63e965fe-57f5-4a8d-b2bc-64088609b7d2" xlink:to="loc_srt_ProductsAndServicesDomain_63e965fe-57f5-4a8d-b2bc-64088609b7d2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_cd43f8f3-fac6-42a5-a331-53b60afeaaec" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_63e965fe-57f5-4a8d-b2bc-64088609b7d2" xlink:to="loc_srt_ProductsAndServicesDomain_cd43f8f3-fac6-42a5-a331-53b60afeaaec" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepositAccountMember_7a88c98f-e975-4786-9b6c-2ed5bc63a67f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DepositAccountMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_cd43f8f3-fac6-42a5-a331-53b60afeaaec" xlink:to="loc_us-gaap_DepositAccountMember_7a88c98f-e975-4786-9b6c-2ed5bc63a67f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiduciaryAndTrustMember_3f0c5ce7-0596-403a-bba2-cf1665504e4f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FiduciaryAndTrustMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_cd43f8f3-fac6-42a5-a331-53b60afeaaec" xlink:to="loc_us-gaap_FiduciaryAndTrustMember_3f0c5ce7-0596-403a-bba2-cf1665504e4f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CardServicesRevenueMember_77558b60-52e3-4417-bcdd-d869d24227e5" xlink:href="efsc-20221231.xsd#efsc_CardServicesRevenueMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_cd43f8f3-fac6-42a5-a331-53b60afeaaec" xlink:to="loc_efsc_CardServicesRevenueMember_77558b60-52e3-4417-bcdd-d869d24227e5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_TaxcreditactivitynetMember_fdb72908-a3ca-49f8-ab79-819d88ab7543" xlink:href="efsc-20221231.xsd#efsc_TaxcreditactivitynetMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_cd43f8f3-fac6-42a5-a331-53b60afeaaec" xlink:to="loc_efsc_TaxcreditactivitynetMember_fdb72908-a3ca-49f8-ab79-819d88ab7543" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_OtherRealEstateMember_dd89cd7c-3176-4e9c-b525-e46bfc5e1bb5" xlink:href="efsc-20221231.xsd#efsc_OtherRealEstateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_cd43f8f3-fac6-42a5-a331-53b60afeaaec" xlink:to="loc_efsc_OtherRealEstateMember_dd89cd7c-3176-4e9c-b525-e46bfc5e1bb5" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialServiceOtherMember_b6fb66c6-3c83-478f-b1e0-c09d686eb93b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancialServiceOtherMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_cd43f8f3-fac6-42a5-a331-53b60afeaaec" xlink:to="loc_us-gaap_FinancialServiceOtherMember_b6fb66c6-3c83-478f-b1e0-c09d686eb93b" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_f0b4110c-adb8-4055-a6ed-c96ac99338c6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_a360c443-8dce-4606-913a-68ecf38514ff" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_f0b4110c-adb8-4055-a6ed-c96ac99338c6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_f0b4110c-adb8-4055-a6ed-c96ac99338c6_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_f0b4110c-adb8-4055-a6ed-c96ac99338c6" xlink:to="loc_us-gaap_EquityComponentDomain_f0b4110c-adb8-4055-a6ed-c96ac99338c6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_d00aae25-f2d2-4db8-8c89-03a10a5a20c0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_f0b4110c-adb8-4055-a6ed-c96ac99338c6" xlink:to="loc_us-gaap_EquityComponentDomain_d00aae25-f2d2-4db8-8c89-03a10a5a20c0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementScenarioAxis_62b243df-d12a-45ae-8e70-d562abcd6b9e" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_StatementScenarioAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_a360c443-8dce-4606-913a-68ecf38514ff" xlink:to="loc_srt_StatementScenarioAxis_62b243df-d12a-45ae-8e70-d562abcd6b9e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_62b243df-d12a-45ae-8e70-d562abcd6b9e_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementScenarioAxis_62b243df-d12a-45ae-8e70-d562abcd6b9e" xlink:to="loc_srt_ScenarioUnspecifiedDomain_62b243df-d12a-45ae-8e70-d562abcd6b9e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_c62778e0-ea16-4b34-8902-69b35fd83b7c" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementScenarioAxis_62b243df-d12a-45ae-8e70-d562abcd6b9e" xlink:to="loc_srt_ScenarioUnspecifiedDomain_c62778e0-ea16-4b34-8902-69b35fd83b7c" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/ConsolidatedStatementsofShareholdersEquity" xlink:type="simple" xlink:href="efsc-20221231.xsd#ConsolidatedStatementsofShareholdersEquity"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/ConsolidatedStatementsofShareholdersEquity" xlink:type="extended" id="i0ff5ddba6e7046bf951b9fafa5737be8_ConsolidatedStatementsofShareholdersEquity">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_bb188cdd-cabc-451c-a897-4dafdacd54c0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesOutstanding_5f692e33-a04e-443f-9098-412f6ae58c37" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PreferredStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_bb188cdd-cabc-451c-a897-4dafdacd54c0" xlink:to="loc_us-gaap_PreferredStockSharesOutstanding_5f692e33-a04e-443f-9098-412f6ae58c37" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_6aba802a-5e21-4aa8-a760-ee3b4c91dbb8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_bb188cdd-cabc-451c-a897-4dafdacd54c0" xlink:to="loc_us-gaap_CommonStockSharesOutstanding_6aba802a-5e21-4aa8-a760-ee3b4c91dbb8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_435f8b2e-64ac-4afa-bde9-97a9c5b96426" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_bb188cdd-cabc-451c-a897-4dafdacd54c0" xlink:to="loc_us-gaap_StockholdersEquity_435f8b2e-64ac-4afa-bde9-97a9c5b96426" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_09fb36bb-ae5b-4d12-b266-a7dfe0771c5e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_bb188cdd-cabc-451c-a897-4dafdacd54c0" xlink:to="loc_us-gaap_NetIncomeLoss_09fb36bb-ae5b-4d12-b266-a7dfe0771c5e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_4fc6f446-69f2-4615-82a1-566aa306fca2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_bb188cdd-cabc-451c-a897-4dafdacd54c0" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_4fc6f446-69f2-4615-82a1-566aa306fca2" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DividendsCommonStockCash_73f1b8e8-fb9e-42ed-9356-54e66c3dd80e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DividendsCommonStockCash"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_bb188cdd-cabc-451c-a897-4dafdacd54c0" xlink:to="loc_us-gaap_DividendsCommonStockCash_73f1b8e8-fb9e-42ed-9356-54e66c3dd80e" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DividendsPreferredStockCash_3a7ffb2b-0dc1-4673-ac31-12655c17df69" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DividendsPreferredStockCash"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_bb188cdd-cabc-451c-a897-4dafdacd54c0" xlink:to="loc_us-gaap_DividendsPreferredStockCash_3a7ffb2b-0dc1-4673-ac31-12655c17df69" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockRepurchasedDuringPeriodShares_fb3e40fc-d327-4c9d-9d3f-5a4e1d3bdcdd" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockRepurchasedDuringPeriodShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_bb188cdd-cabc-451c-a897-4dafdacd54c0" xlink:to="loc_us-gaap_StockRepurchasedDuringPeriodShares_fb3e40fc-d327-4c9d-9d3f-5a4e1d3bdcdd" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockRepurchasedDuringPeriodValue_e411d719-c00d-409a-8f00-5a8c67555249" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockRepurchasedDuringPeriodValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_bb188cdd-cabc-451c-a897-4dafdacd54c0" xlink:to="loc_us-gaap_StockRepurchasedDuringPeriodValue_e411d719-c00d-409a-8f00-5a8c67555249" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensation_b746dead-8f13-45f7-a33f-02b7f316f09a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_bb188cdd-cabc-451c-a897-4dafdacd54c0" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensation_b746dead-8f13-45f7-a33f-02b7f316f09a" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueShareBasedCompensation_0f4e7103-766f-403f-9015-1d46bee7217f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockIssuedDuringPeriodValueShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_bb188cdd-cabc-451c-a897-4dafdacd54c0" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueShareBasedCompensation_0f4e7103-766f-403f-9015-1d46bee7217f" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesAcquisitions_62670c1f-3b50-4f35-b016-87e8da51e82d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesAcquisitions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_bb188cdd-cabc-451c-a897-4dafdacd54c0" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesAcquisitions_62670c1f-3b50-4f35-b016-87e8da51e82d" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_Sharesissuedinconnectionwithacquisition_270cae8b-100c-429b-a4fd-e203d9ef2aa7" xlink:href="efsc-20221231.xsd#efsc_Sharesissuedinconnectionwithacquisition"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_bb188cdd-cabc-451c-a897-4dafdacd54c0" xlink:to="loc_efsc_Sharesissuedinconnectionwithacquisition_270cae8b-100c-429b-a4fd-e203d9ef2aa7" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesNewIssues_b879b604-81ef-44e0-831d-e2e74347e9d8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesNewIssues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_bb188cdd-cabc-451c-a897-4dafdacd54c0" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesNewIssues_b879b604-81ef-44e0-831d-e2e74347e9d8" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueNewIssues_7ec97847-1016-4f03-82d8-0d30427f91ad" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockIssuedDuringPeriodValueNewIssues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_bb188cdd-cabc-451c-a897-4dafdacd54c0" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueNewIssues_7ec97847-1016-4f03-82d8-0d30427f91ad" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalShareBasedCompensationRestrictedStockUnitsRequisiteServicePeriodRecognition_d7b8f5e1-fc09-47d3-8140-57653fd9ca74" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalShareBasedCompensationRestrictedStockUnitsRequisiteServicePeriodRecognition"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_bb188cdd-cabc-451c-a897-4dafdacd54c0" xlink:to="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalShareBasedCompensationRestrictedStockUnitsRequisiteServicePeriodRecognition_d7b8f5e1-fc09-47d3-8140-57653fd9ca74" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_Reclassificationfortheadoptionofnewaccountingstandards_dde0a0cd-d2ba-4e33-abaa-a58b8b12fed7" xlink:href="efsc-20221231.xsd#efsc_Reclassificationfortheadoptionofnewaccountingstandards"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_bb188cdd-cabc-451c-a897-4dafdacd54c0" xlink:to="loc_efsc_Reclassificationfortheadoptionofnewaccountingstandards_dde0a0cd-d2ba-4e33-abaa-a58b8b12fed7" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockRetiredCostMethodAmount_c9d6b41f-d383-4ba0-b5b9-5e509407c855" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TreasuryStockRetiredCostMethodAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_bb188cdd-cabc-451c-a897-4dafdacd54c0" xlink:to="loc_us-gaap_TreasuryStockRetiredCostMethodAmount_c9d6b41f-d383-4ba0-b5b9-5e509407c855" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesOutstanding_739c851b-c768-45f2-ba46-dbbf069dc212" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PreferredStockSharesOutstanding"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_4907a69b-004e-41a0-89d4-3364983979ff" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_4402f5c8-de1e-4d37-8834-294a0e1bd490" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_766dd3e5-71a3-49fb-8e67-fbba54e7be32" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_bb188cdd-cabc-451c-a897-4dafdacd54c0" xlink:to="loc_us-gaap_StatementTable_766dd3e5-71a3-49fb-8e67-fbba54e7be32" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_9f777b6e-bb3d-4974-b945-175c25dc0d6c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_766dd3e5-71a3-49fb-8e67-fbba54e7be32" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_9f777b6e-bb3d-4974-b945-175c25dc0d6c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_9f777b6e-bb3d-4974-b945-175c25dc0d6c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_9f777b6e-bb3d-4974-b945-175c25dc0d6c" xlink:to="loc_us-gaap_EquityComponentDomain_9f777b6e-bb3d-4974-b945-175c25dc0d6c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_6282fb06-7d58-4254-b86e-0eb5f7660e81" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_9f777b6e-bb3d-4974-b945-175c25dc0d6c" xlink:to="loc_us-gaap_EquityComponentDomain_6282fb06-7d58-4254-b86e-0eb5f7660e81" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockMember_1445afd5-bc4a-473a-aec7-437a0d4f1304" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_6282fb06-7d58-4254-b86e-0eb5f7660e81" xlink:to="loc_us-gaap_PreferredStockMember_1445afd5-bc4a-473a-aec7-437a0d4f1304" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_dbdd2590-9c11-4c3b-8a31-885e10c24734" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_6282fb06-7d58-4254-b86e-0eb5f7660e81" xlink:to="loc_us-gaap_CommonStockMember_dbdd2590-9c11-4c3b-8a31-885e10c24734" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockMember_168fc6d6-a5c5-4c71-8de7-100659de69ae" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TreasuryStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_6282fb06-7d58-4254-b86e-0eb5f7660e81" xlink:to="loc_us-gaap_TreasuryStockMember_168fc6d6-a5c5-4c71-8de7-100659de69ae" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalMember_cb412703-3b24-44b1-9aad-172901fdc893" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AdditionalPaidInCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_6282fb06-7d58-4254-b86e-0eb5f7660e81" xlink:to="loc_us-gaap_AdditionalPaidInCapitalMember_cb412703-3b24-44b1-9aad-172901fdc893" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsMember_b0cdde0c-7817-4612-aba7-f7b582815632" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RetainedEarningsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_6282fb06-7d58-4254-b86e-0eb5f7660e81" xlink:to="loc_us-gaap_RetainedEarningsMember_b0cdde0c-7817-4612-aba7-f7b582815632" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_fd580b1c-f03e-4f5d-9e14-31e5554fb3d1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_6282fb06-7d58-4254-b86e-0eb5f7660e81" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_fd580b1c-f03e-4f5d-9e14-31e5554fb3d1" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/ConsolidatedStatementsofShareholdersEquityParenthetical" xlink:type="simple" xlink:href="efsc-20221231.xsd#ConsolidatedStatementsofShareholdersEquityParenthetical"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/ConsolidatedStatementsofShareholdersEquityParenthetical" xlink:type="extended" id="i177928ab389542f5a3a5b50b97b7c227_ConsolidatedStatementsofShareholdersEquityParenthetical">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_339cfb73-24d5-45ba-b584-17dc9decd964" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockDividendsPerShareCashPaid_829824ce-3c03-4072-b0e8-e157a31afb2f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommonStockDividendsPerShareCashPaid"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_339cfb73-24d5-45ba-b584-17dc9decd964" xlink:to="loc_us-gaap_CommonStockDividendsPerShareCashPaid_829824ce-3c03-4072-b0e8-e157a31afb2f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_StockIssuedDuringPeriodSharesAcquisitionsGross_160a07f7-99fc-46d1-962e-dde07888f37f" xlink:href="efsc-20221231.xsd#efsc_StockIssuedDuringPeriodSharesAcquisitionsGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_339cfb73-24d5-45ba-b584-17dc9decd964" xlink:to="loc_efsc_StockIssuedDuringPeriodSharesAcquisitionsGross_160a07f7-99fc-46d1-962e-dde07888f37f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsOfStockIssuanceCosts_7617c8e7-1130-4af4-bf2d-acb670f50823" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PaymentsOfStockIssuanceCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_339cfb73-24d5-45ba-b584-17dc9decd964" xlink:to="loc_us-gaap_PaymentsOfStockIssuanceCosts_7617c8e7-1130-4af4-bf2d-acb670f50823" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockDividendsPerShareCashPaid_eb1a3d0c-011d-4ff3-98de-8be28cf3efca" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PreferredStockDividendsPerShareCashPaid"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_339cfb73-24d5-45ba-b584-17dc9decd964" xlink:to="loc_us-gaap_PreferredStockDividendsPerShareCashPaid_eb1a3d0c-011d-4ff3-98de-8be28cf3efca" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockSharesRetired_5c24490a-fa2b-41c6-8125-df49c3858edc" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TreasuryStockSharesRetired"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_339cfb73-24d5-45ba-b584-17dc9decd964" xlink:to="loc_us-gaap_TreasuryStockSharesRetired_5c24490a-fa2b-41c6-8125-df49c3858edc" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_bb268685-5d65-4610-bb70-b77bceb09b1c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_339cfb73-24d5-45ba-b584-17dc9decd964" xlink:to="loc_us-gaap_NetIncomeLoss_bb268685-5d65-4610-bb70-b77bceb09b1c" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_8191ac9c-4688-4586-9c74-b0287733fd2b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_339cfb73-24d5-45ba-b584-17dc9decd964" xlink:to="loc_us-gaap_StatementTable_8191ac9c-4688-4586-9c74-b0287733fd2b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementScenarioAxis_440c1af4-a1df-4bb6-aa80-5844d0bc83dc" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_StatementScenarioAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_8191ac9c-4688-4586-9c74-b0287733fd2b" xlink:to="loc_srt_StatementScenarioAxis_440c1af4-a1df-4bb6-aa80-5844d0bc83dc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_440c1af4-a1df-4bb6-aa80-5844d0bc83dc_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementScenarioAxis_440c1af4-a1df-4bb6-aa80-5844d0bc83dc" xlink:to="loc_srt_ScenarioUnspecifiedDomain_440c1af4-a1df-4bb6-aa80-5844d0bc83dc_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_7805ae5b-85dd-41c6-937a-4e4f45cc55c7" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementScenarioAxis_440c1af4-a1df-4bb6-aa80-5844d0bc83dc" xlink:to="loc_srt_ScenarioUnspecifiedDomain_7805ae5b-85dd-41c6-937a-4e4f45cc55c7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_e7d8b8cb-cdb0-426f-961d-401b0c5169cd" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_8191ac9c-4688-4586-9c74-b0287733fd2b" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_e7d8b8cb-cdb0-426f-961d-401b0c5169cd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_e7d8b8cb-cdb0-426f-961d-401b0c5169cd_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_e7d8b8cb-cdb0-426f-961d-401b0c5169cd" xlink:to="loc_us-gaap_EquityComponentDomain_e7d8b8cb-cdb0-426f-961d-401b0c5169cd_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_a8cd8cbb-e869-4397-ba7d-8e57efa8053e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_e7d8b8cb-cdb0-426f-961d-401b0c5169cd" xlink:to="loc_us-gaap_EquityComponentDomain_a8cd8cbb-e869-4397-ba7d-8e57efa8053e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsMember_9f0fbbd5-9d15-477c-afdb-a65fbf6c54f5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RetainedEarningsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_a8cd8cbb-e869-4397-ba7d-8e57efa8053e" xlink:to="loc_us-gaap_RetainedEarningsMember_9f0fbbd5-9d15-477c-afdb-a65fbf6c54f5" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/ConsolidatedStatementsofCashFlows" xlink:type="simple" xlink:href="efsc-20221231.xsd#ConsolidatedStatementsofCashFlows"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/ConsolidatedStatementsofCashFlows" xlink:type="extended" id="i76994726b09d43a8bb32d631a16f6100_ConsolidatedStatementsofCashFlows">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_f78efc57-20ed-4b30-9235-02054d472e3a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_c426f3ef-a814-4e88-8ecc-bee94c37fe92" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_f78efc57-20ed-4b30-9235-02054d472e3a" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_c426f3ef-a814-4e88-8ecc-bee94c37fe92" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_d29515c0-8def-442a-b21a-b64434254c73" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_c426f3ef-a814-4e88-8ecc-bee94c37fe92" xlink:to="loc_us-gaap_NetIncomeLoss_d29515c0-8def-442a-b21a-b64434254c73" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_55291144-529a-4020-af62-f7610585d623" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_c426f3ef-a814-4e88-8ecc-bee94c37fe92" xlink:to="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_55291144-529a-4020-af62-f7610585d623" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepreciationNonproduction_d38f32fb-d798-47a7-9ae7-64df00995223" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DepreciationNonproduction"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_55291144-529a-4020-af62-f7610585d623" xlink:to="loc_us-gaap_DepreciationNonproduction_d38f32fb-d798-47a7-9ae7-64df00995223" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProvisionForLoanAndLeaseLosses_852b8c67-3381-4761-98b6-44119a0d87e9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ProvisionForLoanAndLeaseLosses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_55291144-529a-4020-af62-f7610585d623" xlink:to="loc_us-gaap_ProvisionForLoanAndLeaseLosses_852b8c67-3381-4761-98b6-44119a0d87e9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredIncomeTaxExpenseBenefit_a0d63b33-7c60-451f-b40a-6dc0ac2ab44a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DeferredIncomeTaxExpenseBenefit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_55291144-529a-4020-af62-f7610585d623" xlink:to="loc_us-gaap_DeferredIncomeTaxExpenseBenefit_a0d63b33-7c60-451f-b40a-6dc0ac2ab44a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccretionAmortizationOfDiscountsAndPremiumsInvestments_e8908e74-c3b8-4cd0-b75c-a4de93f8a15d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AccretionAmortizationOfDiscountsAndPremiumsInvestments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_55291144-529a-4020-af62-f7610585d623" xlink:to="loc_us-gaap_AccretionAmortizationOfDiscountsAndPremiumsInvestments_e8908e74-c3b8-4cd0-b75c-a4de93f8a15d" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_NetAccretionOfLoanDiscountAndIndemnificationAsset_93a148f4-ecfd-4a91-8d1b-55d75794826b" xlink:href="efsc-20221231.xsd#efsc_NetAccretionOfLoanDiscountAndIndemnificationAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_55291144-529a-4020-af62-f7610585d623" xlink:to="loc_efsc_NetAccretionOfLoanDiscountAndIndemnificationAsset_93a148f4-ecfd-4a91-8d1b-55d75794826b" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AmortizationOfIntangibleAssets_07fa6787-fa92-4737-b1f2-376648c40aac" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AmortizationOfIntangibleAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_55291144-529a-4020-af62-f7610585d623" xlink:to="loc_us-gaap_AmortizationOfIntangibleAssets_07fa6787-fa92-4737-b1f2-376648c40aac" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_AmortizationOfServicingAssets_f64be0b5-066e-4079-b123-53ca6761fb64" xlink:href="efsc-20221231.xsd#efsc_AmortizationOfServicingAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_55291144-529a-4020-af62-f7610585d623" xlink:to="loc_efsc_AmortizationOfServicingAssets_f64be0b5-066e-4079-b123-53ca6761fb64" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsForOriginationOfMortgageLoansHeldForSale_f7dc0823-9bf0-4ec3-859a-6e9d27f66668" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PaymentsForOriginationOfMortgageLoansHeldForSale"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_55291144-529a-4020-af62-f7610585d623" xlink:to="loc_us-gaap_PaymentsForOriginationOfMortgageLoansHeldForSale_f7dc0823-9bf0-4ec3-859a-6e9d27f66668" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromSaleOfMortgageLoansHeldForSale_9472fb91-fb39-4fd8-8536-c48fe5d22817" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ProceedsFromSaleOfMortgageLoansHeldForSale"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_55291144-529a-4020-af62-f7610585d623" xlink:to="loc_us-gaap_ProceedsFromSaleOfMortgageLoansHeldForSale_9472fb91-fb39-4fd8-8536-c48fe5d22817" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GainLossOnSaleOfInvestments_4c248e3a-ec48-484d-a9cc-c10eace9ce2d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_GainLossOnSaleOfInvestments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_55291144-529a-4020-af62-f7610585d623" xlink:to="loc_us-gaap_GainLossOnSaleOfInvestments_4c248e3a-ec48-484d-a9cc-c10eace9ce2d" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GainLossOnSaleOfProperties_7ba1c8cc-5108-4926-9f1d-ba542d1b6a07" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_GainLossOnSaleOfProperties"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_55291144-529a-4020-af62-f7610585d623" xlink:to="loc_us-gaap_GainLossOnSaleOfProperties_7ba1c8cc-5108-4926-9f1d-ba542d1b6a07" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GainLossOnSaleOfPropertyPlantEquipment_a6ae5f34-d092-4e92-84c1-72468bcb0f4b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_GainLossOnSaleOfPropertyPlantEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_55291144-529a-4020-af62-f7610585d623" xlink:to="loc_us-gaap_GainLossOnSaleOfPropertyPlantEquipment_a6ae5f34-d092-4e92-84c1-72468bcb0f4b" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_GainOnStateTaxCreditsNet_eefc4c69-0ccb-4458-b626-badf88d70b58" xlink:href="efsc-20221231.xsd#efsc_GainOnStateTaxCreditsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_55291144-529a-4020-af62-f7610585d623" xlink:to="loc_efsc_GainOnStateTaxCreditsNet_eefc4c69-0ccb-4458-b626-badf88d70b58" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetImpairmentCharges_c1387370-78e3-4f7a-b24d-51c6dbb5609d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AssetImpairmentCharges"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_55291144-529a-4020-af62-f7610585d623" xlink:to="loc_us-gaap_AssetImpairmentCharges_c1387370-78e3-4f7a-b24d-51c6dbb5609d" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensation_8f6ec17a-3f9d-42a0-aa8f-749b13ab5a22" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_55291144-529a-4020-af62-f7610585d623" xlink:to="loc_us-gaap_ShareBasedCompensation_8f6ec17a-3f9d-42a0-aa8f-749b13ab5a22" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInOtherOperatingCapitalNet_2b0a5818-da21-4159-b3ff-daecc432a542" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncreaseDecreaseInOtherOperatingCapitalNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_55291144-529a-4020-af62-f7610585d623" xlink:to="loc_us-gaap_IncreaseDecreaseInOtherOperatingCapitalNet_2b0a5818-da21-4159-b3ff-daecc432a542" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_5067e62d-f546-40a8-8abc-dac7adaae372" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_c426f3ef-a814-4e88-8ecc-bee94c37fe92" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_5067e62d-f546-40a8-8abc-dac7adaae372" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_bd405ae6-80da-48ef-abd8-c35643e67fd8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_f78efc57-20ed-4b30-9235-02054d472e3a" xlink:to="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_bd405ae6-80da-48ef-abd8-c35643e67fd8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireBusinessesNetOfCashAcquired_aed2b3f4-2c3f-41a7-bced-a38fc7f9c6d6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PaymentsToAcquireBusinessesNetOfCashAcquired"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_bd405ae6-80da-48ef-abd8-c35643e67fd8" xlink:to="loc_us-gaap_PaymentsToAcquireBusinessesNetOfCashAcquired_aed2b3f4-2c3f-41a7-bced-a38fc7f9c6d6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsForProceedsFromLoansAndLeases_62113b60-4a06-45ae-82e2-dd23dac8d371" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PaymentsForProceedsFromLoansAndLeases"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_bd405ae6-80da-48ef-abd8-c35643e67fd8" xlink:to="loc_us-gaap_PaymentsForProceedsFromLoansAndLeases_62113b60-4a06-45ae-82e2-dd23dac8d371" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromSaleOfAvailableForSaleSecuritiesDebt_2ba086a8-5b74-4a93-bda2-7c3dbd0b7f47" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ProceedsFromSaleOfAvailableForSaleSecuritiesDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_bd405ae6-80da-48ef-abd8-c35643e67fd8" xlink:to="loc_us-gaap_ProceedsFromSaleOfAvailableForSaleSecuritiesDebt_2ba086a8-5b74-4a93-bda2-7c3dbd0b7f47" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromMaturitiesPrepaymentsAndCallsOfAvailableForSaleSecurities_00343aa3-0505-482e-9d4c-280ec0d80053" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ProceedsFromMaturitiesPrepaymentsAndCallsOfAvailableForSaleSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_bd405ae6-80da-48ef-abd8-c35643e67fd8" xlink:to="loc_us-gaap_ProceedsFromMaturitiesPrepaymentsAndCallsOfAvailableForSaleSecurities_00343aa3-0505-482e-9d4c-280ec0d80053" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromSaleAndMaturityOfHeldToMaturitySecurities_9ffcdd07-78f8-4c22-8db0-820641e17def" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ProceedsFromSaleAndMaturityOfHeldToMaturitySecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_bd405ae6-80da-48ef-abd8-c35643e67fd8" xlink:to="loc_us-gaap_ProceedsFromSaleAndMaturityOfHeldToMaturitySecurities_9ffcdd07-78f8-4c22-8db0-820641e17def" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromMaturitiesPrepaymentsAndCallsOfOtherInvestments_6144bc3f-f36b-4511-86db-947a58542967" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ProceedsFromMaturitiesPrepaymentsAndCallsOfOtherInvestments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_bd405ae6-80da-48ef-abd8-c35643e67fd8" xlink:to="loc_us-gaap_ProceedsFromMaturitiesPrepaymentsAndCallsOfOtherInvestments_6144bc3f-f36b-4511-86db-947a58542967" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ProceedsFromSaleOfStateTaxCreditsHeldForSale_ffff2136-0242-45d8-9814-84dd6c40a289" xlink:href="efsc-20221231.xsd#efsc_ProceedsFromSaleOfStateTaxCreditsHeldForSale"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_bd405ae6-80da-48ef-abd8-c35643e67fd8" xlink:to="loc_efsc_ProceedsFromSaleOfStateTaxCreditsHeldForSale_ffff2136-0242-45d8-9814-84dd6c40a289" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromSaleOfOtherRealEstate_c66974b2-b54d-4bd2-8cc5-171f5ce86285" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ProceedsFromSaleOfOtherRealEstate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_bd405ae6-80da-48ef-abd8-c35643e67fd8" xlink:to="loc_us-gaap_ProceedsFromSaleOfOtherRealEstate_c66974b2-b54d-4bd2-8cc5-171f5ce86285" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromSaleOfPropertyPlantAndEquipment_f7c860c1-e949-4386-a85d-b85450082331" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ProceedsFromSaleOfPropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_bd405ae6-80da-48ef-abd8-c35643e67fd8" xlink:to="loc_us-gaap_ProceedsFromSaleOfPropertyPlantAndEquipment_f7c860c1-e949-4386-a85d-b85450082331" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromLifeInsurancePolicies_b6d8b1bf-1550-4eb2-afa1-188a8374f578" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ProceedsFromLifeInsurancePolicies"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_bd405ae6-80da-48ef-abd8-c35643e67fd8" xlink:to="loc_us-gaap_ProceedsFromLifeInsurancePolicies_b6d8b1bf-1550-4eb2-afa1-188a8374f578" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireInvestmentsAbstract_0431dd22-18b5-4fd0-9c8e-e7cad7a896ff" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PaymentsToAcquireInvestmentsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_bd405ae6-80da-48ef-abd8-c35643e67fd8" xlink:to="loc_us-gaap_PaymentsToAcquireInvestmentsAbstract_0431dd22-18b5-4fd0-9c8e-e7cad7a896ff" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireAvailableForSaleSecuritiesDebt_c6dcf0a2-7e44-4169-9fca-ef23eefb2a1f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PaymentsToAcquireAvailableForSaleSecuritiesDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PaymentsToAcquireInvestmentsAbstract_0431dd22-18b5-4fd0-9c8e-e7cad7a896ff" xlink:to="loc_us-gaap_PaymentsToAcquireAvailableForSaleSecuritiesDebt_c6dcf0a2-7e44-4169-9fca-ef23eefb2a1f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireHeldToMaturitySecurities_b96d60c8-9e56-4585-9cd6-92b1ea7f422b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PaymentsToAcquireHeldToMaturitySecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PaymentsToAcquireInvestmentsAbstract_0431dd22-18b5-4fd0-9c8e-e7cad7a896ff" xlink:to="loc_us-gaap_PaymentsToAcquireHeldToMaturitySecurities_b96d60c8-9e56-4585-9cd6-92b1ea7f422b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireOtherInvestments_17c3c600-aa60-4bb6-a132-b4985da75e25" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PaymentsToAcquireOtherInvestments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PaymentsToAcquireInvestmentsAbstract_0431dd22-18b5-4fd0-9c8e-e7cad7a896ff" xlink:to="loc_us-gaap_PaymentsToAcquireOtherInvestments_17c3c600-aa60-4bb6-a132-b4985da75e25" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_PaymentsToAcquireStateTaxCreditsHeldForSale_03e76deb-26af-418f-943a-5845145b91e1" xlink:href="efsc-20221231.xsd#efsc_PaymentsToAcquireStateTaxCreditsHeldForSale"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PaymentsToAcquireInvestmentsAbstract_0431dd22-18b5-4fd0-9c8e-e7cad7a896ff" xlink:to="loc_efsc_PaymentsToAcquireStateTaxCreditsHeldForSale_03e76deb-26af-418f-943a-5845145b91e1" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_008a8bd1-c6b8-403b-958e-cd23d0895c34" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PaymentsToAcquirePropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PaymentsToAcquireInvestmentsAbstract_0431dd22-18b5-4fd0-9c8e-e7cad7a896ff" xlink:to="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_008a8bd1-c6b8-403b-958e-cd23d0895c34" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_131a07a6-1b50-4e01-86b9-4aceb15e34d9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_bd405ae6-80da-48ef-abd8-c35643e67fd8" xlink:to="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_131a07a6-1b50-4e01-86b9-4aceb15e34d9" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_799b088e-aff1-422b-95fe-2fa54d094c54" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_f78efc57-20ed-4b30-9235-02054d472e3a" xlink:to="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_799b088e-aff1-422b-95fe-2fa54d094c54" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetChangeNoninterestBearingDepositsDomestic_1d500086-410b-40b1-b019-678d99443097" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetChangeNoninterestBearingDepositsDomestic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_799b088e-aff1-422b-95fe-2fa54d094c54" xlink:to="loc_us-gaap_NetChangeNoninterestBearingDepositsDomestic_1d500086-410b-40b1-b019-678d99443097" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetChangeInterestBearingDepositsDomestic_baef0a48-0f27-48ef-ab46-a9ec64a713c7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetChangeInterestBearingDepositsDomestic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_799b088e-aff1-422b-95fe-2fa54d094c54" xlink:to="loc_us-gaap_NetChangeInterestBearingDepositsDomestic_baef0a48-0f27-48ef-ab46-a9ec64a713c7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOfSubordinatedLongTermDebt_2852b107-d72a-4673-82e3-c0d1674f41e8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ProceedsFromIssuanceOfSubordinatedLongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_799b088e-aff1-422b-95fe-2fa54d094c54" xlink:to="loc_us-gaap_ProceedsFromIssuanceOfSubordinatedLongTermDebt_2852b107-d72a-4673-82e3-c0d1674f41e8" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RepaymentsOfSubordinatedDebt_6f331676-9179-4cf1-90e4-727d065a3303" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RepaymentsOfSubordinatedDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_799b088e-aff1-422b-95fe-2fa54d094c54" xlink:to="loc_us-gaap_RepaymentsOfSubordinatedDebt_6f331676-9179-4cf1-90e4-727d065a3303" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ProceedsfromPaymentsforShortTermFHLBankBorrowingsFinancingActivities_aa153ecf-d73f-4149-94d3-311b75609b37" xlink:href="efsc-20221231.xsd#efsc_ProceedsfromPaymentsforShortTermFHLBankBorrowingsFinancingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_799b088e-aff1-422b-95fe-2fa54d094c54" xlink:to="loc_efsc_ProceedsfromPaymentsforShortTermFHLBankBorrowingsFinancingActivities_aa153ecf-d73f-4149-94d3-311b75609b37" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_RepaymentsOfLongTermFederalHomeLoanBankAdvances_babce806-5355-44da-a094-e513934934d5" xlink:href="efsc-20221231.xsd#efsc_RepaymentsOfLongTermFederalHomeLoanBankAdvances"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_799b088e-aff1-422b-95fe-2fa54d094c54" xlink:to="loc_efsc_RepaymentsOfLongTermFederalHomeLoanBankAdvances_babce806-5355-44da-a094-e513934934d5" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_RepaymentOfPPPLFAdvances_13b493c4-87c4-4e09-b9b6-00649f533d1b" xlink:href="efsc-20221231.xsd#efsc_RepaymentOfPPPLFAdvances"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_799b088e-aff1-422b-95fe-2fa54d094c54" xlink:to="loc_efsc_RepaymentOfPPPLFAdvances_13b493c4-87c4-4e09-b9b6-00649f533d1b" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RepaymentsOfNotesPayable_d126928c-1b62-4738-85da-ba4b8266ec99" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RepaymentsOfNotesPayable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_799b088e-aff1-422b-95fe-2fa54d094c54" xlink:to="loc_us-gaap_RepaymentsOfNotesPayable_d126928c-1b62-4738-85da-ba4b8266ec99" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromPaymentsForInSecuritiesSoldUnderAgreementsToRepurchase_3d1cf68e-cc1a-472d-9995-0178a4af3c4c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ProceedsFromPaymentsForInSecuritiesSoldUnderAgreementsToRepurchase"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_799b088e-aff1-422b-95fe-2fa54d094c54" xlink:to="loc_us-gaap_ProceedsFromPaymentsForInSecuritiesSoldUnderAgreementsToRepurchase_3d1cf68e-cc1a-472d-9995-0178a4af3c4c" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsOfDividendsCommonStock_78c06bec-f4bb-4716-b8e7-be2a36bbc0d1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PaymentsOfDividendsCommonStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_799b088e-aff1-422b-95fe-2fa54d094c54" xlink:to="loc_us-gaap_PaymentsOfDividendsCommonStock_78c06bec-f4bb-4716-b8e7-be2a36bbc0d1" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsForRepurchaseOfCommonStock_e7352847-193e-4ffe-b1df-966319b3c704" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PaymentsForRepurchaseOfCommonStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_799b088e-aff1-422b-95fe-2fa54d094c54" xlink:to="loc_us-gaap_PaymentsForRepurchaseOfCommonStock_e7352847-193e-4ffe-b1df-966319b3c704" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsOfDividendsPreferredStockAndPreferenceStock_8f02b4dd-9185-432c-8b29-5017b6230bc3" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PaymentsOfDividendsPreferredStockAndPreferenceStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_799b088e-aff1-422b-95fe-2fa54d094c54" xlink:to="loc_us-gaap_PaymentsOfDividendsPreferredStockAndPreferenceStock_8f02b4dd-9185-432c-8b29-5017b6230bc3" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOfPreferredStockAndPreferenceStock_cb4c8f2b-9504-42b2-8067-1bbc30f44546" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ProceedsFromIssuanceOfPreferredStockAndPreferenceStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_799b088e-aff1-422b-95fe-2fa54d094c54" xlink:to="loc_us-gaap_ProceedsFromIssuanceOfPreferredStockAndPreferenceStock_cb4c8f2b-9504-42b2-8067-1bbc30f44546" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNoncashIncomeExpense_60c6ccbf-f495-45c9-a431-0ffff4a9265f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherNoncashIncomeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_799b088e-aff1-422b-95fe-2fa54d094c54" xlink:to="loc_us-gaap_OtherNoncashIncomeExpense_60c6ccbf-f495-45c9-a431-0ffff4a9265f" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_96257e46-85c0-4fc6-8432-c1123e050081" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_799b088e-aff1-422b-95fe-2fa54d094c54" xlink:to="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_96257e46-85c0-4fc6-8432-c1123e050081" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_cf961d4b-a4e3-46b3-9a7e-c6f5c9bd87d1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_f78efc57-20ed-4b30-9235-02054d472e3a" xlink:to="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_cf961d4b-a4e3-46b3-9a7e-c6f5c9bd87d1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_c551ab72-2e6d-4ff3-8df1-6d5b4235ec87" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_f78efc57-20ed-4b30-9235-02054d472e3a" xlink:to="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_c551ab72-2e6d-4ff3-8df1-6d5b4235ec87" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_75cc4bf0-64eb-4130-b549-56f8c9d7db34" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SupplementalCashFlowInformationAbstract_6fc89905-21d0-42af-8115-28e7a9459e19" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SupplementalCashFlowInformationAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_f78efc57-20ed-4b30-9235-02054d472e3a" xlink:to="loc_us-gaap_SupplementalCashFlowInformationAbstract_6fc89905-21d0-42af-8115-28e7a9459e19" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestPaidNet_e6d52497-7f05-406d-956e-c1401dbed019" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestPaidNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SupplementalCashFlowInformationAbstract_6fc89905-21d0-42af-8115-28e7a9459e19" xlink:to="loc_us-gaap_InterestPaidNet_e6d52497-7f05-406d-956e-c1401dbed019" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxesPaidNet_d553cac3-1bb9-47d9-8105-67658aa0c2c7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeTaxesPaidNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SupplementalCashFlowInformationAbstract_6fc89905-21d0-42af-8115-28e7a9459e19" xlink:to="loc_us-gaap_IncomeTaxesPaidNet_d553cac3-1bb9-47d9-8105-67658aa0c2c7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NoncashInvestingAndFinancingItemsAbstract_e6616d95-7e3f-4ed4-a34e-00e000d5dedc" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NoncashInvestingAndFinancingItemsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_f78efc57-20ed-4b30-9235-02054d472e3a" xlink:to="loc_us-gaap_NoncashInvestingAndFinancingItemsAbstract_e6616d95-7e3f-4ed4-a34e-00e000d5dedc" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransferToOtherRealEstate_b9192886-eecd-42cc-a5c8-dfa0edab8876" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TransferToOtherRealEstate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoncashInvestingAndFinancingItemsAbstract_e6616d95-7e3f-4ed4-a34e-00e000d5dedc" xlink:to="loc_us-gaap_TransferToOtherRealEstate_b9192886-eecd-42cc-a5c8-dfa0edab8876" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_SalesOfOtherRealEstateFinanced_0605e48e-bc94-4a00-830c-51b004bc1efb" xlink:href="efsc-20221231.xsd#efsc_SalesOfOtherRealEstateFinanced"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoncashInvestingAndFinancingItemsAbstract_e6616d95-7e3f-4ed4-a34e-00e000d5dedc" xlink:to="loc_efsc_SalesOfOtherRealEstateFinanced_0605e48e-bc94-4a00-830c-51b004bc1efb" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransferToInvestments_fcc15a5d-f3da-459b-8463-8351ad1ce397" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TransferToInvestments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoncashInvestingAndFinancingItemsAbstract_e6616d95-7e3f-4ed4-a34e-00e000d5dedc" xlink:to="loc_us-gaap_TransferToInvestments_fcc15a5d-f3da-459b-8463-8351ad1ce397" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_TransferFromFixedAssetsToLoans_c2780795-a4d1-4e92-8426-8fb9d83f2dd5" xlink:href="efsc-20221231.xsd#efsc_TransferFromFixedAssetsToLoans"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoncashInvestingAndFinancingItemsAbstract_e6616d95-7e3f-4ed4-a34e-00e000d5dedc" xlink:to="loc_efsc_TransferFromFixedAssetsToLoans_c2780795-a4d1-4e92-8426-8fb9d83f2dd5" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RightOfUseAssetObtainedInExchangeForOperatingLeaseLiability_a2f5be88-adad-46ad-9969-7a5feeec2b32" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RightOfUseAssetObtainedInExchangeForOperatingLeaseLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoncashInvestingAndFinancingItemsAbstract_e6616d95-7e3f-4ed4-a34e-00e000d5dedc" xlink:to="loc_us-gaap_RightOfUseAssetObtainedInExchangeForOperatingLeaseLiability_a2f5be88-adad-46ad-9969-7a5feeec2b32" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesAcquisitions_0fcff028-57e1-44e3-901b-a3e9b3920966" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesAcquisitions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoncashInvestingAndFinancingItemsAbstract_e6616d95-7e3f-4ed4-a34e-00e000d5dedc" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesAcquisitions_0fcff028-57e1-44e3-901b-a3e9b3920966" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_3ec05f2e-4c5d-41cf-8246-0c2921bc4861" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_f78efc57-20ed-4b30-9235-02054d472e3a" xlink:to="loc_us-gaap_StatementTable_3ec05f2e-4c5d-41cf-8246-0c2921bc4861" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementScenarioAxis_2de8a7cc-f3f5-4bed-8e28-de1a60a7acc0" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_StatementScenarioAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_3ec05f2e-4c5d-41cf-8246-0c2921bc4861" xlink:to="loc_srt_StatementScenarioAxis_2de8a7cc-f3f5-4bed-8e28-de1a60a7acc0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_2de8a7cc-f3f5-4bed-8e28-de1a60a7acc0_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementScenarioAxis_2de8a7cc-f3f5-4bed-8e28-de1a60a7acc0" xlink:to="loc_srt_ScenarioUnspecifiedDomain_2de8a7cc-f3f5-4bed-8e28-de1a60a7acc0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_088d5d2c-33cc-446a-bf44-713855efb2a4" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementScenarioAxis_2de8a7cc-f3f5-4bed-8e28-de1a60a7acc0" xlink:to="loc_srt_ScenarioUnspecifiedDomain_088d5d2c-33cc-446a-bf44-713855efb2a4" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/ParentCompanyOnlyCondensedFinancialStatementsParentheticalDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#ParentCompanyOnlyCondensedFinancialStatementsParentheticalDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/ParentCompanyOnlyCondensedFinancialStatementsParentheticalDetails" xlink:type="extended" id="icb24d380cc8b4939ad2e6eede7131d7c_ParentCompanyOnlyCondensedFinancialStatementsParentheticalDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_8132b06e-ed14-4159-bbe6-d6de350f92b5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CollateralPledged_95d0e5c0-2460-4406-9ff8-a1a5b3c5aa1c" xlink:href="efsc-20221231.xsd#efsc_CollateralPledged"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_8132b06e-ed14-4159-bbe6-d6de350f92b5" xlink:to="loc_efsc_CollateralPledged_95d0e5c0-2460-4406-9ff8-a1a5b3c5aa1c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_06194067-c2aa-4d24-9a61-b19e10a30b54" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_8132b06e-ed14-4159-bbe6-d6de350f92b5" xlink:to="loc_us-gaap_StatementTable_06194067-c2aa-4d24-9a61-b19e10a30b54" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidatedEntitiesAxis_720ebd10-832a-49c3-a5c7-196f511fcd2d" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ConsolidatedEntitiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_06194067-c2aa-4d24-9a61-b19e10a30b54" xlink:to="loc_srt_ConsolidatedEntitiesAxis_720ebd10-832a-49c3-a5c7-196f511fcd2d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidatedEntitiesDomain_720ebd10-832a-49c3-a5c7-196f511fcd2d_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ConsolidatedEntitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ConsolidatedEntitiesAxis_720ebd10-832a-49c3-a5c7-196f511fcd2d" xlink:to="loc_srt_ConsolidatedEntitiesDomain_720ebd10-832a-49c3-a5c7-196f511fcd2d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidatedEntitiesDomain_dbc5a4d0-60d0-4160-ac55-f30b404c7578" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ConsolidatedEntitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ConsolidatedEntitiesAxis_720ebd10-832a-49c3-a5c7-196f511fcd2d" xlink:to="loc_srt_ConsolidatedEntitiesDomain_dbc5a4d0-60d0-4160-ac55-f30b404c7578" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ParentCompanyMember_4311ec15-1873-4e70-8e6f-0dca656673f7" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ParentCompanyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidatedEntitiesDomain_dbc5a4d0-60d0-4160-ac55-f30b404c7578" xlink:to="loc_srt_ParentCompanyMember_4311ec15-1873-4e70-8e6f-0dca656673f7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RestatementAxis_15d0d39e-cc4c-4c91-a8dd-e23abc1859e6" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RestatementAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_06194067-c2aa-4d24-9a61-b19e10a30b54" xlink:to="loc_srt_RestatementAxis_15d0d39e-cc4c-4c91-a8dd-e23abc1859e6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RestatementDomain_15d0d39e-cc4c-4c91-a8dd-e23abc1859e6_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RestatementDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RestatementAxis_15d0d39e-cc4c-4c91-a8dd-e23abc1859e6" xlink:to="loc_srt_RestatementDomain_15d0d39e-cc4c-4c91-a8dd-e23abc1859e6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RestatementDomain_76d9bcef-15a4-46e5-92fd-bd12746035b7" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RestatementDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RestatementAxis_15d0d39e-cc4c-4c91-a8dd-e23abc1859e6" xlink:to="loc_srt_RestatementDomain_76d9bcef-15a4-46e5-92fd-bd12746035b7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_7922de4f-4540-4166-bd9c-e17b432b9b2c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_06194067-c2aa-4d24-9a61-b19e10a30b54" xlink:to="loc_us-gaap_StatementClassOfStockAxis_7922de4f-4540-4166-bd9c-e17b432b9b2c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_7922de4f-4540-4166-bd9c-e17b432b9b2c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_7922de4f-4540-4166-bd9c-e17b432b9b2c" xlink:to="loc_us-gaap_ClassOfStockDomain_7922de4f-4540-4166-bd9c-e17b432b9b2c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_85ac88a4-c7d2-4b77-ac54-23ae3600a6f2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_7922de4f-4540-4166-bd9c-e17b432b9b2c" xlink:to="loc_us-gaap_ClassOfStockDomain_85ac88a4-c7d2-4b77-ac54-23ae3600a6f2" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/PortfolioLoansTables" xlink:type="simple" xlink:href="efsc-20221231.xsd#PortfolioLoansTables"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/PortfolioLoansTables" xlink:type="extended" id="i9b5399f6d91142f1934cd87634bb72d0_PortfolioLoansTables">
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CoveredLoansLineItems_f21346d7-7891-4119-b2f9-4dd989205fd5" xlink:href="efsc-20221231.xsd#efsc_CoveredLoansLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ScheduleOfAccountsNotesLoansAndFinancingReceivableExcludingAllowanceForCreditLossesTableTextBlock_18084ae8-65e3-4d6c-af10-ef9984f15afd" xlink:href="efsc-20221231.xsd#efsc_ScheduleOfAccountsNotesLoansAndFinancingReceivableExcludingAllowanceForCreditLossesTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_efsc_CoveredLoansLineItems_f21346d7-7891-4119-b2f9-4dd989205fd5" xlink:to="loc_efsc_ScheduleOfAccountsNotesLoansAndFinancingReceivableExcludingAllowanceForCreditLossesTableTextBlock_18084ae8-65e3-4d6c-af10-ef9984f15afd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfCreditLossesRelatedToFinancingReceivablesNoncurrentTableTextBlock_167e9e41-3830-4138-a5d1-954b56ea03b8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfCreditLossesRelatedToFinancingReceivablesNoncurrentTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_efsc_CoveredLoansLineItems_f21346d7-7891-4119-b2f9-4dd989205fd5" xlink:to="loc_us-gaap_ScheduleOfCreditLossesRelatedToFinancingReceivablesNoncurrentTableTextBlock_167e9e41-3830-4138-a5d1-954b56ea03b8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFinancingReceivablesNonAccrualStatusTableTextBlock_442634ae-9100-4cb1-8e7c-e0c7047ad9b1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfFinancingReceivablesNonAccrualStatusTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_efsc_CoveredLoansLineItems_f21346d7-7891-4119-b2f9-4dd989205fd5" xlink:to="loc_us-gaap_ScheduleOfFinancingReceivablesNonAccrualStatusTableTextBlock_442634ae-9100-4cb1-8e7c-e0c7047ad9b1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ScheduleOfCollateralDependentLoansTableTextBlock_fe3edda3-fe78-401f-b202-b05db927be38" xlink:href="efsc-20221231.xsd#efsc_ScheduleOfCollateralDependentLoansTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_efsc_CoveredLoansLineItems_f21346d7-7891-4119-b2f9-4dd989205fd5" xlink:to="loc_efsc_ScheduleOfCollateralDependentLoansTableTextBlock_fe3edda3-fe78-401f-b202-b05db927be38" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TroubledDebtRestructuringsOnFinancingReceivablesTableTextBlock_19b6ecc4-e30e-49b7-bf23-d45d3a12f75c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TroubledDebtRestructuringsOnFinancingReceivablesTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_efsc_CoveredLoansLineItems_f21346d7-7891-4119-b2f9-4dd989205fd5" xlink:to="loc_us-gaap_TroubledDebtRestructuringsOnFinancingReceivablesTableTextBlock_19b6ecc4-e30e-49b7-bf23-d45d3a12f75c" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ScheduleOfFinancingReceivableTroubledDebtRestructuringsSubsequentDefaultsTableTextBlock_89e4ccda-1e59-4c85-b09a-f33525da76bf" xlink:href="efsc-20221231.xsd#efsc_ScheduleOfFinancingReceivableTroubledDebtRestructuringsSubsequentDefaultsTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_efsc_CoveredLoansLineItems_f21346d7-7891-4119-b2f9-4dd989205fd5" xlink:to="loc_efsc_ScheduleOfFinancingReceivableTroubledDebtRestructuringsSubsequentDefaultsTableTextBlock_89e4ccda-1e59-4c85-b09a-f33525da76bf" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PastDueFinancingReceivablesTableTextBlock_a4611a4b-9366-4a7a-807e-1605f2bec6c8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PastDueFinancingReceivablesTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_efsc_CoveredLoansLineItems_f21346d7-7891-4119-b2f9-4dd989205fd5" xlink:to="loc_us-gaap_PastDueFinancingReceivablesTableTextBlock_a4611a4b-9366-4a7a-807e-1605f2bec6c8" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableCreditQualityIndicatorsTableTextBlock_0a7716c3-44da-4b41-803c-b6033d9a463c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableCreditQualityIndicatorsTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_efsc_CoveredLoansLineItems_f21346d7-7891-4119-b2f9-4dd989205fd5" xlink:to="loc_us-gaap_FinancingReceivableCreditQualityIndicatorsTableTextBlock_0a7716c3-44da-4b41-803c-b6033d9a463c" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_AcquiredPCDLoansTableTextBlock_9997e4c5-ce1c-4207-b6dc-53fa38bd9e6a" xlink:href="efsc-20221231.xsd#efsc_AcquiredPCDLoansTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_efsc_CoveredLoansLineItems_f21346d7-7891-4119-b2f9-4dd989205fd5" xlink:to="loc_efsc_AcquiredPCDLoansTableTextBlock_9997e4c5-ce1c-4207-b6dc-53fa38bd9e6a" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CoveredLoansTable_f2864571-1b93-4a08-8b36-6093ec09f013" xlink:href="efsc-20221231.xsd#efsc_CoveredLoansTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_efsc_CoveredLoansLineItems_f21346d7-7891-4119-b2f9-4dd989205fd5" xlink:to="loc_efsc_CoveredLoansTable_f2864571-1b93-4a08-8b36-6093ec09f013" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansByMajorCategoryAxis_1200063d-fe83-494c-bbce-840bb759f479" xlink:href="efsc-20221231.xsd#efsc_LoansByMajorCategoryAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_efsc_CoveredLoansTable_f2864571-1b93-4a08-8b36-6093ec09f013" xlink:to="loc_efsc_LoansByMajorCategoryAxis_1200063d-fe83-494c-bbce-840bb759f479" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansByMajorCategoryDomain_1200063d-fe83-494c-bbce-840bb759f479_default" xlink:href="efsc-20221231.xsd#efsc_LoansByMajorCategoryDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_efsc_LoansByMajorCategoryAxis_1200063d-fe83-494c-bbce-840bb759f479" xlink:to="loc_efsc_LoansByMajorCategoryDomain_1200063d-fe83-494c-bbce-840bb759f479_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansByMajorCategoryDomain_ad3decbf-ffcd-4ea6-9c1f-25f8f7fc23b3" xlink:href="efsc-20221231.xsd#efsc_LoansByMajorCategoryDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_efsc_LoansByMajorCategoryAxis_1200063d-fe83-494c-bbce-840bb759f479" xlink:to="loc_efsc_LoansByMajorCategoryDomain_ad3decbf-ffcd-4ea6-9c1f-25f8f7fc23b3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_NoncoveredLoansMember_750943c0-f3cd-4b4f-8e7c-7273a49ec2fc" xlink:href="efsc-20221231.xsd#efsc_NoncoveredLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_efsc_LoansByMajorCategoryDomain_ad3decbf-ffcd-4ea6-9c1f-25f8f7fc23b3" xlink:to="loc_efsc_NoncoveredLoansMember_750943c0-f3cd-4b4f-8e7c-7273a49ec2fc" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/OtherBorrowingsandNotesPayableTables" xlink:type="simple" xlink:href="efsc-20221231.xsd#OtherBorrowingsandNotesPayableTables"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/OtherBorrowingsandNotesPayableTables" xlink:type="extended" id="i898c47b4ab894073b01cf39673f026b1_OtherBorrowingsandNotesPayableTables">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_ee72f56f-557f-44b2-be5e-811f18e89d63" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfDebtInstrumentsTextBlock_09d3bacf-4d69-454a-9eb9-b39e5286071a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfDebtInstrumentsTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_ee72f56f-557f-44b2-be5e-811f18e89d63" xlink:to="loc_us-gaap_ScheduleOfDebtInstrumentsTextBlock_09d3bacf-4d69-454a-9eb9-b39e5286071a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfDebtTableTextBlock_c23ada14-6eca-4e72-ac22-d0d7137d607e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfDebtTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_ee72f56f-557f-44b2-be5e-811f18e89d63" xlink:to="loc_us-gaap_ScheduleOfDebtTableTextBlock_c23ada14-6eca-4e72-ac22-d0d7137d607e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfLineOfCreditFacilitiesTextBlock_0f1498d8-acc4-43b0-829e-591f077ae033" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfLineOfCreditFacilitiesTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_ee72f56f-557f-44b2-be5e-811f18e89d63" xlink:to="loc_us-gaap_ScheduleOfLineOfCreditFacilitiesTextBlock_0f1498d8-acc4-43b0-829e-591f077ae033" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_80e2aa54-a13d-4833-82d5-39e54cf39819" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_ee72f56f-557f-44b2-be5e-811f18e89d63" xlink:to="loc_us-gaap_DebtInstrumentTable_80e2aa54-a13d-4833-82d5-39e54cf39819" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_a65d5300-4cce-4c5a-a8b3-ba038d9d4f6a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_80e2aa54-a13d-4833-82d5-39e54cf39819" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_a65d5300-4cce-4c5a-a8b3-ba038d9d4f6a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_a65d5300-4cce-4c5a-a8b3-ba038d9d4f6a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_a65d5300-4cce-4c5a-a8b3-ba038d9d4f6a" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_a65d5300-4cce-4c5a-a8b3-ba038d9d4f6a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_28617b7a-5915-466c-8b93-f44d3e24bdca" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_a65d5300-4cce-4c5a-a8b3-ba038d9d4f6a" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_28617b7a-5915-466c-8b93-f44d3e24bdca" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BorrowingsMember_c4941bdd-5643-44bb-a867-5f0548788202" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_BorrowingsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_28617b7a-5915-466c-8b93-f44d3e24bdca" xlink:to="loc_us-gaap_BorrowingsMember_c4941bdd-5643-44bb-a867-5f0548788202" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/CompensationPlansTables" xlink:type="simple" xlink:href="efsc-20221231.xsd#CompensationPlansTables"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/CompensationPlansTables" xlink:type="extended" id="i21c32cbbb6314da7bf9ce06703b516c2_CompensationPlansTables">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ea0ff664-ef1e-4cf5-8f9c-c3d8563ba3c8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfCompensationCostForShareBasedPaymentArrangementsAllocationOfShareBasedCompensationCostsByPlanTableTextBlock_f079c33a-8a29-46bf-883c-7395df5aa3a0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfCompensationCostForShareBasedPaymentArrangementsAllocationOfShareBasedCompensationCostsByPlanTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ea0ff664-ef1e-4cf5-8f9c-c3d8563ba3c8" xlink:to="loc_us-gaap_ScheduleOfCompensationCostForShareBasedPaymentArrangementsAllocationOfShareBasedCompensationCostsByPlanTableTextBlock_f079c33a-8a29-46bf-883c-7395df5aa3a0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_OutstandingLongTermIncentiveAwardsTableTextBlock_7c1a2d17-fa65-4200-9880-9af555c2c158" xlink:href="efsc-20221231.xsd#efsc_OutstandingLongTermIncentiveAwardsTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ea0ff664-ef1e-4cf5-8f9c-c3d8563ba3c8" xlink:to="loc_efsc_OutstandingLongTermIncentiveAwardsTableTextBlock_7c1a2d17-fa65-4200-9880-9af555c2c158" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisclosureOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTextBlock_e0297526-e287-4f12-8302-5337bffc94c7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DisclosureOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ea0ff664-ef1e-4cf5-8f9c-c3d8563ba3c8" xlink:to="loc_us-gaap_DisclosureOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTextBlock_e0297526-e287-4f12-8302-5337bffc94c7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationActivityTableTextBlock_4c5d9215-e866-4bba-b915-f4865f45473f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfShareBasedCompensationActivityTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ea0ff664-ef1e-4cf5-8f9c-c3d8563ba3c8" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationActivityTableTextBlock_4c5d9215-e866-4bba-b915-f4865f45473f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationRestrictedStockUnitsAwardActivityTableTextBlock_1e980613-6c71-400c-b64e-8417ca3c869f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfShareBasedCompensationRestrictedStockUnitsAwardActivityTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ea0ff664-ef1e-4cf5-8f9c-c3d8563ba3c8" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationRestrictedStockUnitsAwardActivityTableTextBlock_1e980613-6c71-400c-b64e-8417ca3c869f" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAccumulatedOtherComprehensiveIncomeLossTableTextBlock_08082a57-a45b-4b41-b739-2746702ebcf6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfAccumulatedOtherComprehensiveIncomeLossTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ea0ff664-ef1e-4cf5-8f9c-c3d8563ba3c8" xlink:to="loc_us-gaap_ScheduleOfAccumulatedOtherComprehensiveIncomeLossTableTextBlock_08082a57-a45b-4b41-b739-2746702ebcf6" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAmountsRecognizedInOtherComprehensiveIncomeLossTableTextBlock_15854426-2425-4c57-909a-d4c99a8e5327" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfAmountsRecognizedInOtherComprehensiveIncomeLossTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ea0ff664-ef1e-4cf5-8f9c-c3d8563ba3c8" xlink:to="loc_us-gaap_ScheduleOfAmountsRecognizedInOtherComprehensiveIncomeLossTableTextBlock_15854426-2425-4c57-909a-d4c99a8e5327" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_f9905d9d-a7f9-4043-a31d-8629dcee5493" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_ea0ff664-ef1e-4cf5-8f9c-c3d8563ba3c8" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_f9905d9d-a7f9-4043-a31d-8629dcee5493" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameAxis_5c8390f5-f43f-4e2b-b0d6-941d8a6b91ba" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PlanNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_f9905d9d-a7f9-4043-a31d-8629dcee5493" xlink:to="loc_us-gaap_PlanNameAxis_5c8390f5-f43f-4e2b-b0d6-941d8a6b91ba" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_5c8390f5-f43f-4e2b-b0d6-941d8a6b91ba_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PlanNameAxis_5c8390f5-f43f-4e2b-b0d6-941d8a6b91ba" xlink:to="loc_us-gaap_PlanNameDomain_5c8390f5-f43f-4e2b-b0d6-941d8a6b91ba_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_8df0170e-0ecd-402b-b8e4-c2d23d068c2b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PlanNameAxis_5c8390f5-f43f-4e2b-b0d6-941d8a6b91ba" xlink:to="loc_us-gaap_PlanNameDomain_8df0170e-0ecd-402b-b8e4-c2d23d068c2b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_StockPlanForNonManagementDirectorsMember_24334cf7-3e47-4bf2-ac83-a01bca54dc97" xlink:href="efsc-20221231.xsd#efsc_StockPlanForNonManagementDirectorsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_8df0170e-0ecd-402b-b8e4-c2d23d068c2b" xlink:to="loc_efsc_StockPlanForNonManagementDirectorsMember_24334cf7-3e47-4bf2-ac83-a01bca54dc97" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_461d6f1d-aff5-4d4c-894a-f765129fca5c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_f9905d9d-a7f9-4043-a31d-8629dcee5493" xlink:to="loc_us-gaap_AwardTypeAxis_461d6f1d-aff5-4d4c-894a-f765129fca5c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_461d6f1d-aff5-4d4c-894a-f765129fca5c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_461d6f1d-aff5-4d4c-894a-f765129fca5c" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_461d6f1d-aff5-4d4c-894a-f765129fca5c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_937d02e6-9eea-4d53-9d28-e4f1ac1d1c00" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_461d6f1d-aff5-4d4c-894a-f765129fca5c" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_937d02e6-9eea-4d53-9d28-e4f1ac1d1c00" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_StockOptionsAndStockSettledStockAppreciationRightsMember_abb2c74b-7653-4d2f-8b4d-3c292d1d3cbb" xlink:href="efsc-20221231.xsd#efsc_StockOptionsAndStockSettledStockAppreciationRightsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_937d02e6-9eea-4d53-9d28-e4f1ac1d1c00" xlink:to="loc_efsc_StockOptionsAndStockSettledStockAppreciationRightsMember_abb2c74b-7653-4d2f-8b4d-3c292d1d3cbb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_acce4bc7-d335-4ad2-ad2b-7d2833ba4a16" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_937d02e6-9eea-4d53-9d28-e4f1ac1d1c00" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_acce4bc7-d335-4ad2-ad2b-7d2833ba4a16" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/SummaryofSignificantAccountingPoliciesDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#SummaryofSignificantAccountingPoliciesDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/SummaryofSignificantAccountingPoliciesDetails" xlink:type="extended" id="id819d21fccb64406a642c5b9e6409790_SummaryofSignificantAccountingPoliciesDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentLineItems_6365d23e-e500-42e4-8249-ed176763e16a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseRightOfUseAsset_bcea2030-390d-4239-814c-1a3f3a021369" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OperatingLeaseRightOfUseAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_6365d23e-e500-42e4-8249-ed176763e16a" xlink:to="loc_us-gaap_OperatingLeaseRightOfUseAsset_bcea2030-390d-4239-814c-1a3f3a021369" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NumberOfReportableSegments_91f73e1f-c0a5-45c1-9766-6ac66b0db9d2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NumberOfReportableSegments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_6365d23e-e500-42e4-8249-ed176763e16a" xlink:to="loc_us-gaap_NumberOfReportableSegments_91f73e1f-c0a5-45c1-9766-6ac66b0db9d2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentUsefulLife_eb2656aa-8ea2-4fae-a7db-ce06e6650bba" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PropertyPlantAndEquipmentUsefulLife"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_6365d23e-e500-42e4-8249-ed176763e16a" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentUsefulLife_eb2656aa-8ea2-4fae-a7db-ce06e6650bba" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_882ef27f-5ed0-417a-a281-c3f2d5f0113c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetUsefulLife"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_6365d23e-e500-42e4-8249-ed176763e16a" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_882ef27f-5ed0-417a-a281-c3f2d5f0113c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_TaxCreditsRightToReceiveAtAgreedUponRatesDuration_b39d1ce9-219b-4402-b8ca-1089041702ed" xlink:href="efsc-20221231.xsd#efsc_TaxCreditsRightToReceiveAtAgreedUponRatesDuration"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_6365d23e-e500-42e4-8249-ed176763e16a" xlink:to="loc_efsc_TaxCreditsRightToReceiveAtAgreedUponRatesDuration_b39d1ce9-219b-4402-b8ca-1089041702ed" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiability_f40f0e1a-fe15-4b1e-b58f-019989e725b4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OperatingLeaseLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_6365d23e-e500-42e4-8249-ed176763e16a" xlink:to="loc_us-gaap_OperatingLeaseLiability_f40f0e1a-fe15-4b1e-b58f-019989e725b4" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ServicingAsset_df0f6d71-f597-40c2-a1fd-feef521b20eb" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ServicingAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_6365d23e-e500-42e4-8249-ed176763e16a" xlink:to="loc_us-gaap_ServicingAsset_df0f6d71-f597-40c2-a1fd-feef521b20eb" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestReceivable_2ff28ab7-b460-4b45-9293-e9a6ee7a0388" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestReceivable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_6365d23e-e500-42e4-8249-ed176763e16a" xlink:to="loc_us-gaap_InterestReceivable_2ff28ab7-b460-4b45-9293-e9a6ee7a0388" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_51e2844c-fd22-4b2d-9098-7adeae08e32f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_6365d23e-e500-42e4-8249-ed176763e16a" xlink:to="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_51e2844c-fd22-4b2d-9098-7adeae08e32f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityAxis_7ec95b13-6d5a-4cb6-85f6-b7bb63e77482" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeTaxAuthorityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_51e2844c-fd22-4b2d-9098-7adeae08e32f" xlink:to="loc_us-gaap_IncomeTaxAuthorityAxis_7ec95b13-6d5a-4cb6-85f6-b7bb63e77482" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityDomain_7ec95b13-6d5a-4cb6-85f6-b7bb63e77482_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeTaxAuthorityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeTaxAuthorityAxis_7ec95b13-6d5a-4cb6-85f6-b7bb63e77482" xlink:to="loc_us-gaap_IncomeTaxAuthorityDomain_7ec95b13-6d5a-4cb6-85f6-b7bb63e77482_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityDomain_2a8fb08a-845e-4a91-b404-0b8a4808ada3" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeTaxAuthorityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeTaxAuthorityAxis_7ec95b13-6d5a-4cb6-85f6-b7bb63e77482" xlink:to="loc_us-gaap_IncomeTaxAuthorityDomain_2a8fb08a-845e-4a91-b404-0b8a4808ada3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StateAndLocalJurisdictionMember_8340f1bf-de3d-4d76-8647-e3b303b4d96a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StateAndLocalJurisdictionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeTaxAuthorityDomain_2a8fb08a-845e-4a91-b404-0b8a4808ada3" xlink:to="loc_us-gaap_StateAndLocalJurisdictionMember_8340f1bf-de3d-4d76-8647-e3b303b4d96a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_e329c0eb-dc01-4593-b8fa-9d5c79be1b15" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_51e2844c-fd22-4b2d-9098-7adeae08e32f" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_e329c0eb-dc01-4593-b8fa-9d5c79be1b15" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_e329c0eb-dc01-4593-b8fa-9d5c79be1b15_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_e329c0eb-dc01-4593-b8fa-9d5c79be1b15" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_e329c0eb-dc01-4593-b8fa-9d5c79be1b15_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_da3a695d-b97b-4443-9255-f675fe1c3a94" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_e329c0eb-dc01-4593-b8fa-9d5c79be1b15" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_da3a695d-b97b-4443-9255-f675fe1c3a94" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CoreDepositsMember_cdf248bd-3849-4719-a923-2eba67a896d2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CoreDepositsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_da3a695d-b97b-4443-9255-f675fe1c3a94" xlink:to="loc_us-gaap_CoreDepositsMember_cdf248bd-3849-4719-a923-2eba67a896d2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_957f61fa-f0ce-4eb2-a6dd-58f08b3ae745" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_51e2844c-fd22-4b2d-9098-7adeae08e32f" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_957f61fa-f0ce-4eb2-a6dd-58f08b3ae745" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_957f61fa-f0ce-4eb2-a6dd-58f08b3ae745_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_957f61fa-f0ce-4eb2-a6dd-58f08b3ae745" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_957f61fa-f0ce-4eb2-a6dd-58f08b3ae745_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_b79538a2-324e-4fff-9a93-a716f9378e04" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_957f61fa-f0ce-4eb2-a6dd-58f08b3ae745" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_b79538a2-324e-4fff-9a93-a716f9378e04" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_FurnitureFixturesAndEquipmentMember_92aaaa4c-fc12-4839-91e8-42379abea37f" xlink:href="efsc-20221231.xsd#efsc_FurnitureFixturesAndEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_b79538a2-324e-4fff-9a93-a716f9378e04" xlink:to="loc_efsc_FurnitureFixturesAndEquipmentMember_92aaaa4c-fc12-4839-91e8-42379abea37f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BuildingAndBuildingImprovementsMember_45916407-6064-4f51-83e2-696e4ff32967" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_BuildingAndBuildingImprovementsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_b79538a2-324e-4fff-9a93-a716f9378e04" xlink:to="loc_us-gaap_BuildingAndBuildingImprovementsMember_45916407-6064-4f51-83e2-696e4ff32967" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_7a5ac0d6-525c-4fc0-9a67-e119b9a90663" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_51e2844c-fd22-4b2d-9098-7adeae08e32f" xlink:to="loc_srt_RangeAxis_7a5ac0d6-525c-4fc0-9a67-e119b9a90663" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_7a5ac0d6-525c-4fc0-9a67-e119b9a90663_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_7a5ac0d6-525c-4fc0-9a67-e119b9a90663" xlink:to="loc_srt_RangeMember_7a5ac0d6-525c-4fc0-9a67-e119b9a90663_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_1bc85681-c058-4633-b5bb-37caeb8ae54b" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_7a5ac0d6-525c-4fc0-9a67-e119b9a90663" xlink:to="loc_srt_RangeMember_1bc85681-c058-4633-b5bb-37caeb8ae54b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_0627c037-f256-484d-a14d-02d589db5fd8" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_1bc85681-c058-4633-b5bb-37caeb8ae54b" xlink:to="loc_srt_MinimumMember_0627c037-f256-484d-a14d-02d589db5fd8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_e0064693-15b6-4b32-9ce3-5e343545abdc" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_1bc85681-c058-4633-b5bb-37caeb8ae54b" xlink:to="loc_srt_MaximumMember_e0064693-15b6-4b32-9ce3-5e343545abdc" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/InvestmentsScheduleofAvailableforsaleandHeldtoMaturitySecuritiesReconciliationDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#InvestmentsScheduleofAvailableforsaleandHeldtoMaturitySecuritiesReconciliationDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/InvestmentsScheduleofAvailableforsaleandHeldtoMaturitySecuritiesReconciliationDetails" xlink:type="extended" id="i923f1d5147e44b068a1a277cbceb7cea_InvestmentsScheduleofAvailableforsaleandHeldtoMaturitySecuritiesReconciliationDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_c9430b68-1f3d-4b9f-a52f-239dbb13e596" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesAbstract_6736ce2e-1296-4be8-ae04-ca9e6ccdc373" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AvailableForSaleSecuritiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_c9430b68-1f3d-4b9f-a52f-239dbb13e596" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesAbstract_6736ce2e-1296-4be8-ae04-ca9e6ccdc373" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis_2c4f8d90-6737-4a78-9b1b-b5b1ae929e50" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesAbstract_6736ce2e-1296-4be8-ae04-ca9e6ccdc373" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis_2c4f8d90-6737-4a78-9b1b-b5b1ae929e50" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax_26ac3d5a-a34c-455c-a084-0fb14188bf9e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesAbstract_6736ce2e-1296-4be8-ae04-ca9e6ccdc373" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax_26ac3d5a-a34c-455c-a084-0fb14188bf9e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax_602b5bf2-4b39-410d-b670-c699ff2c8095" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesAbstract_6736ce2e-1296-4be8-ae04-ca9e6ccdc373" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax_602b5bf2-4b39-410d-b670-c699ff2c8095" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_2b609b6d-651e-4167-bc0a-138f56c2776e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AvailableForSaleSecuritiesDebtSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesAbstract_6736ce2e-1296-4be8-ae04-ca9e6ccdc373" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_2b609b6d-651e-4167-bc0a-138f56c2776e" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesClassifiedAbstract_d05ec0e3-572e-4f90-8566-182d6454065c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_HeldToMaturitySecuritiesClassifiedAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_c9430b68-1f3d-4b9f-a52f-239dbb13e596" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesClassifiedAbstract_d05ec0e3-572e-4f90-8566-182d6454065c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecurities_bcf44e03-f999-4312-8c19-5751da094de2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_HeldToMaturitySecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HeldToMaturitySecuritiesClassifiedAbstract_d05ec0e3-572e-4f90-8566-182d6454065c" xlink:to="loc_us-gaap_HeldToMaturitySecurities_bcf44e03-f999-4312-8c19-5751da094de2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingGain_0203bc50-8ded-4104-8ac1-bc5fbbbd4f7c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingGain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HeldToMaturitySecuritiesClassifiedAbstract_d05ec0e3-572e-4f90-8566-182d6454065c" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingGain_0203bc50-8ded-4104-8ac1-bc5fbbbd4f7c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingLoss_ebc591ad-c412-4d97-8eef-fac83a3844ae" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HeldToMaturitySecuritiesClassifiedAbstract_d05ec0e3-572e-4f90-8566-182d6454065c" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingLoss_ebc591ad-c412-4d97-8eef-fac83a3844ae" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesHeldToMaturityAllowanceForCreditLoss_fb5e7e26-34da-4a68-9686-c6319b2a90e2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtSecuritiesHeldToMaturityAllowanceForCreditLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HeldToMaturitySecuritiesClassifiedAbstract_d05ec0e3-572e-4f90-8566-182d6454065c" xlink:to="loc_us-gaap_DebtSecuritiesHeldToMaturityAllowanceForCreditLoss_fb5e7e26-34da-4a68-9686-c6319b2a90e2" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesFairValue_dcb49557-5da9-4087-b22a-93079ab0129b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_HeldToMaturitySecuritiesFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HeldToMaturitySecuritiesClassifiedAbstract_d05ec0e3-572e-4f90-8566-182d6454065c" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesFairValue_dcb49557-5da9-4087-b22a-93079ab0129b" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_DebtSecuritiesHeldToMaturityNetOfAllowanceForCreditLosses_36d4e4c9-5c7c-46fb-9686-2e659436f578" xlink:href="efsc-20221231.xsd#efsc_DebtSecuritiesHeldToMaturityNetOfAllowanceForCreditLosses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HeldToMaturitySecuritiesClassifiedAbstract_d05ec0e3-572e-4f90-8566-182d6454065c" xlink:to="loc_efsc_DebtSecuritiesHeldToMaturityNetOfAllowanceForCreditLosses_36d4e4c9-5c7c-46fb-9686-2e659436f578" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesTable_f2956836-aeb4-4581-ad11-8a7e138fa7bf" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfHeldToMaturitySecuritiesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_c9430b68-1f3d-4b9f-a52f-239dbb13e596" xlink:to="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesTable_f2956836-aeb4-4581-ad11-8a7e138fa7bf" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_e4849949-4fb4-4878-a5c1-13e86e6ed606" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesTable_f2956836-aeb4-4581-ad11-8a7e138fa7bf" xlink:to="loc_us-gaap_FinancialInstrumentAxis_e4849949-4fb4-4878-a5c1-13e86e6ed606" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_e4849949-4fb4-4878-a5c1-13e86e6ed606_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_e4849949-4fb4-4878-a5c1-13e86e6ed606" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_e4849949-4fb4-4878-a5c1-13e86e6ed606_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_8fd335bc-175f-4bf2-99ac-e99d8e0e88d1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_e4849949-4fb4-4878-a5c1-13e86e6ed606" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_8fd335bc-175f-4bf2-99ac-e99d8e0e88d1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USGovernmentSponsoredEnterprisesDebtSecuritiesMember_2f593d94-07c8-4627-b5aa-e23728fc6155" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_USGovernmentSponsoredEnterprisesDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_8fd335bc-175f-4bf2-99ac-e99d8e0e88d1" xlink:to="loc_us-gaap_USGovernmentSponsoredEnterprisesDebtSecuritiesMember_2f593d94-07c8-4627-b5aa-e23728fc6155" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USStatesAndPoliticalSubdivisionsMember_4448c8a9-5be9-438e-b749-d0dcd8f5a3f8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_USStatesAndPoliticalSubdivisionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_8fd335bc-175f-4bf2-99ac-e99d8e0e88d1" xlink:to="loc_us-gaap_USStatesAndPoliticalSubdivisionsMember_4448c8a9-5be9-438e-b749-d0dcd8f5a3f8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResidentialMortgageBackedSecuritiesMember_3ce14a11-19eb-45a6-9698-0d74948792fa" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ResidentialMortgageBackedSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_8fd335bc-175f-4bf2-99ac-e99d8e0e88d1" xlink:to="loc_us-gaap_ResidentialMortgageBackedSecuritiesMember_3ce14a11-19eb-45a6-9698-0d74948792fa" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USTreasuryBillSecuritiesMember_5f1b7dc5-b470-49e7-af70-c2d77beb0712" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_USTreasuryBillSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_8fd335bc-175f-4bf2-99ac-e99d8e0e88d1" xlink:to="loc_us-gaap_USTreasuryBillSecuritiesMember_5f1b7dc5-b470-49e7-af70-c2d77beb0712" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CorporateDebtSecuritiesMember_e888bdda-d98f-4ef5-b704-5eee97c05209" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CorporateDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_8fd335bc-175f-4bf2-99ac-e99d8e0e88d1" xlink:to="loc_us-gaap_CorporateDebtSecuritiesMember_e888bdda-d98f-4ef5-b704-5eee97c05209" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/InvestmentsScheduleofUnrealizedLossonInvestmentsDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#InvestmentsScheduleofUnrealizedLossonInvestmentsDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/InvestmentsScheduleofUnrealizedLossonInvestmentsDetails" xlink:type="extended" id="if568cb4d90a44fd3bdf16291a0c5d59d_InvestmentsScheduleofUnrealizedLossonInvestmentsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_74b78a39-f585-4a32-85ee-000ce0c74996" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12Months_818ba911-5e94-4dea-8e02-fcf7e3a92bab" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12Months"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_74b78a39-f585-4a32-85ee-000ce0c74996" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12Months_818ba911-5e94-4dea-8e02-fcf7e3a92bab" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12MonthsAccumulatedLoss_8b1532f3-937c-4a61-91ea-4e282795ec52" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12MonthsAccumulatedLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_74b78a39-f585-4a32-85ee-000ce0c74996" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12MonthsAccumulatedLoss_8b1532f3-937c-4a61-91ea-4e282795ec52" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLonger_ce06a4da-2d09-4c69-b8d9-24f866840844" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLonger"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_74b78a39-f585-4a32-85ee-000ce0c74996" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLonger_ce06a4da-2d09-4c69-b8d9-24f866840844" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLongerAccumulatedLoss_c0838afb-a8fe-4053-a302-f6652aab909a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLongerAccumulatedLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_74b78a39-f585-4a32-85ee-000ce0c74996" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLongerAccumulatedLoss_c0838afb-a8fe-4053-a302-f6652aab909a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPosition_0c2c0c4c-7660-4aa6-92fc-f54fe91c2072" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPosition"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_74b78a39-f585-4a32-85ee-000ce0c74996" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPosition_0c2c0c4c-7660-4aa6-92fc-f54fe91c2072" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPositionAccumulatedLoss_f046b7c3-db52-4ae3-ae25-e12ce9a31af4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPositionAccumulatedLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_74b78a39-f585-4a32-85ee-000ce0c74996" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPositionAccumulatedLoss_f046b7c3-db52-4ae3-ae25-e12ce9a31af4" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesTable_206d5f3c-71e0-44ef-bdd7-8bcaf34674c9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfAvailableForSaleSecuritiesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_74b78a39-f585-4a32-85ee-000ce0c74996" xlink:to="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesTable_206d5f3c-71e0-44ef-bdd7-8bcaf34674c9" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_2147a06f-bf81-4d1a-8646-89405e8edd4e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesTable_206d5f3c-71e0-44ef-bdd7-8bcaf34674c9" xlink:to="loc_us-gaap_FinancialInstrumentAxis_2147a06f-bf81-4d1a-8646-89405e8edd4e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_2147a06f-bf81-4d1a-8646-89405e8edd4e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_2147a06f-bf81-4d1a-8646-89405e8edd4e" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_2147a06f-bf81-4d1a-8646-89405e8edd4e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_73fd6181-57b6-4e0a-aa6e-b6870de3c3a7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_2147a06f-bf81-4d1a-8646-89405e8edd4e" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_73fd6181-57b6-4e0a-aa6e-b6870de3c3a7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USGovernmentSponsoredEnterprisesDebtSecuritiesMember_337b6d65-de07-4e5b-a7e5-013a8acefd8b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_USGovernmentSponsoredEnterprisesDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_73fd6181-57b6-4e0a-aa6e-b6870de3c3a7" xlink:to="loc_us-gaap_USGovernmentSponsoredEnterprisesDebtSecuritiesMember_337b6d65-de07-4e5b-a7e5-013a8acefd8b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USStatesAndPoliticalSubdivisionsMember_97006ee1-9459-4d81-81ac-1c32e216b5bc" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_USStatesAndPoliticalSubdivisionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_73fd6181-57b6-4e0a-aa6e-b6870de3c3a7" xlink:to="loc_us-gaap_USStatesAndPoliticalSubdivisionsMember_97006ee1-9459-4d81-81ac-1c32e216b5bc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResidentialMortgageBackedSecuritiesMember_6cc6ecdb-2c2b-4097-af84-06d69ab3c868" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ResidentialMortgageBackedSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_73fd6181-57b6-4e0a-aa6e-b6870de3c3a7" xlink:to="loc_us-gaap_ResidentialMortgageBackedSecuritiesMember_6cc6ecdb-2c2b-4097-af84-06d69ab3c868" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USTreasuryBillSecuritiesMember_aeb84423-29f7-4e6f-8543-c69d7325a774" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_USTreasuryBillSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_73fd6181-57b6-4e0a-aa6e-b6870de3c3a7" xlink:to="loc_us-gaap_USTreasuryBillSecuritiesMember_aeb84423-29f7-4e6f-8543-c69d7325a774" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CorporateDebtSecuritiesMember_f2e729cf-73e2-4838-878e-9472916b12c0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CorporateDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_73fd6181-57b6-4e0a-aa6e-b6870de3c3a7" xlink:to="loc_us-gaap_CorporateDebtSecuritiesMember_f2e729cf-73e2-4838-878e-9472916b12c0" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/InvestmentsNarrativeDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#InvestmentsNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/InvestmentsNarrativeDetails" xlink:type="extended" id="i6199a0679f65469eaddecad91a6fdaec_InvestmentsNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_b711a2d5-2c79-4952-8637-e11b825ca4a5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis_e5295e01-25c8-4647-abd0-b499389ede6d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_b711a2d5-2c79-4952-8637-e11b825ca4a5" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis_e5295e01-25c8-4647-abd0-b499389ede6d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherInvestmentsAndSecuritiesAtCost_7400b3d6-71df-4d47-86e7-887d83cc57b5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherInvestmentsAndSecuritiesAtCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_b711a2d5-2c79-4952-8637-e11b825ca4a5" xlink:to="loc_us-gaap_OtherInvestmentsAndSecuritiesAtCost_7400b3d6-71df-4d47-86e7-887d83cc57b5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPositionNumberOfPositions_2827e578-2751-4d21-b876-5430e3b32afd" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPositionNumberOfPositions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_b711a2d5-2c79-4952-8637-e11b825ca4a5" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPositionNumberOfPositions_2827e578-2751-4d21-b876-5430e3b32afd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax_94645a95-b3fd-4b38-b1a6-fd5b7aa80d22" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_b711a2d5-2c79-4952-8637-e11b825ca4a5" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax_94645a95-b3fd-4b38-b1a6-fd5b7aa80d22" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_MaximumPercentageOfShareholdersEquitySecurityHoldingsHeldOfOneIssuer_340feaf4-ff78-45bb-a67a-63ac6c792818" xlink:href="efsc-20221231.xsd#efsc_MaximumPercentageOfShareholdersEquitySecurityHoldingsHeldOfOneIssuer"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_b711a2d5-2c79-4952-8637-e11b825ca4a5" xlink:to="loc_efsc_MaximumPercentageOfShareholdersEquitySecurityHoldingsHeldOfOneIssuer_340feaf4-ff78-45bb-a67a-63ac6c792818" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecurityOwnedAndPledgedAsCollateralFairValue_52478f6a-0fd4-4094-a866-0741bbe37dc6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SecurityOwnedAndPledgedAsCollateralFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_b711a2d5-2c79-4952-8637-e11b825ca4a5" xlink:to="loc_us-gaap_SecurityOwnedAndPledgedAsCollateralFairValue_52478f6a-0fd4-4094-a866-0741bbe37dc6" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_DebtSecuritiesMortgageBackedSecuritiesWeightedAverageLife_ad5bc98a-8f4f-4fb1-a5b6-cc752ba022a4" xlink:href="efsc-20221231.xsd#efsc_DebtSecuritiesMortgageBackedSecuritiesWeightedAverageLife"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_b711a2d5-2c79-4952-8637-e11b825ca4a5" xlink:to="loc_efsc_DebtSecuritiesMortgageBackedSecuritiesWeightedAverageLife_ad5bc98a-8f4f-4fb1-a5b6-cc752ba022a4" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_AccruedInterestReceivableHeldToMaturitySecurities_ff270591-bff0-4977-93cd-95c45e6b32cf" xlink:href="efsc-20221231.xsd#efsc_AccruedInterestReceivableHeldToMaturitySecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_b711a2d5-2c79-4952-8637-e11b825ca4a5" xlink:to="loc_efsc_AccruedInterestReceivableHeldToMaturitySecurities_ff270591-bff0-4977-93cd-95c45e6b32cf" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesHeldToMaturityAllowanceForCreditLoss_2c2d7d61-e039-4369-b5ea-21c4c8af5679" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtSecuritiesHeldToMaturityAllowanceForCreditLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_b711a2d5-2c79-4952-8637-e11b825ca4a5" xlink:to="loc_us-gaap_DebtSecuritiesHeldToMaturityAllowanceForCreditLoss_2c2d7d61-e039-4369-b5ea-21c4c8af5679" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromSaleOfAvailableForSaleSecuritiesDebt_326f1387-0363-45d1-8ce2-9e18febcea5d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ProceedsFromSaleOfAvailableForSaleSecuritiesDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_b711a2d5-2c79-4952-8637-e11b825ca4a5" xlink:to="loc_us-gaap_ProceedsFromSaleOfAvailableForSaleSecuritiesDebt_326f1387-0363-45d1-8ce2-9e18febcea5d" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleRealizedGain_aa29970c-9859-4aae-9af9-47c35ae1553d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtSecuritiesAvailableForSaleRealizedGain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_b711a2d5-2c79-4952-8637-e11b825ca4a5" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleRealizedGain_aa29970c-9859-4aae-9af9-47c35ae1553d" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_8d8af207-6df9-4fa3-89b7-ff262ca4c0cd" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AvailableForSaleSecuritiesDebtSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_b711a2d5-2c79-4952-8637-e11b825ca4a5" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_8d8af207-6df9-4fa3-89b7-ff262ca4c0cd" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesTable_2bd2095b-07cd-4f52-bbae-4d117a98839b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfAvailableForSaleSecuritiesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_b711a2d5-2c79-4952-8637-e11b825ca4a5" xlink:to="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesTable_2bd2095b-07cd-4f52-bbae-4d117a98839b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InformationByCategoryOfDebtSecurityAxis_6157e8a5-73c5-4dd4-abfc-6c4d1bed0849" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InformationByCategoryOfDebtSecurityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesTable_2bd2095b-07cd-4f52-bbae-4d117a98839b" xlink:to="loc_us-gaap_InformationByCategoryOfDebtSecurityAxis_6157e8a5-73c5-4dd4-abfc-6c4d1bed0849" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherThanTemporaryImpairmentCreditLossesRecognizedInEarningsCategoriesOfInvestmentsDomain_6157e8a5-73c5-4dd4-abfc-6c4d1bed0849_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherThanTemporaryImpairmentCreditLossesRecognizedInEarningsCategoriesOfInvestmentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_InformationByCategoryOfDebtSecurityAxis_6157e8a5-73c5-4dd4-abfc-6c4d1bed0849" xlink:to="loc_us-gaap_OtherThanTemporaryImpairmentCreditLossesRecognizedInEarningsCategoriesOfInvestmentsDomain_6157e8a5-73c5-4dd4-abfc-6c4d1bed0849_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherThanTemporaryImpairmentCreditLossesRecognizedInEarningsCategoriesOfInvestmentsDomain_c2bbd2a1-6d42-49ad-8960-a85efb257952" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherThanTemporaryImpairmentCreditLossesRecognizedInEarningsCategoriesOfInvestmentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_InformationByCategoryOfDebtSecurityAxis_6157e8a5-73c5-4dd4-abfc-6c4d1bed0849" xlink:to="loc_us-gaap_OtherThanTemporaryImpairmentCreditLossesRecognizedInEarningsCategoriesOfInvestmentsDomain_c2bbd2a1-6d42-49ad-8960-a85efb257952" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ReclassifiedtoHeldtoMaturityMember_04c191e6-84be-4ab5-a3c2-121499947ae2" xlink:href="efsc-20221231.xsd#efsc_ReclassifiedtoHeldtoMaturityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherThanTemporaryImpairmentCreditLossesRecognizedInEarningsCategoriesOfInvestmentsDomain_c2bbd2a1-6d42-49ad-8960-a85efb257952" xlink:to="loc_efsc_ReclassifiedtoHeldtoMaturityMember_04c191e6-84be-4ab5-a3c2-121499947ae2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityByLocationAxis_6bea24c4-32ce-4109-86d9-6ae20ea79463" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityByLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesTable_2bd2095b-07cd-4f52-bbae-4d117a98839b" xlink:to="loc_dei_EntityByLocationAxis_6bea24c4-32ce-4109-86d9-6ae20ea79463" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LocationDomain_6bea24c4-32ce-4109-86d9-6ae20ea79463_default" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_LocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_EntityByLocationAxis_6bea24c4-32ce-4109-86d9-6ae20ea79463" xlink:to="loc_dei_LocationDomain_6bea24c4-32ce-4109-86d9-6ae20ea79463_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LocationDomain_9f0560ca-1677-469d-b894-5a2f9cd1cdaa" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_LocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_EntityByLocationAxis_6bea24c4-32ce-4109-86d9-6ae20ea79463" xlink:to="loc_dei_LocationDomain_9f0560ca-1677-469d-b894-5a2f9cd1cdaa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_DesMoinesMember_16230854-3871-4524-8043-c11b940da3ed" xlink:href="efsc-20221231.xsd#efsc_DesMoinesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_LocationDomain_9f0560ca-1677-469d-b894-5a2f9cd1cdaa" xlink:to="loc_efsc_DesMoinesMember_16230854-3871-4524-8043-c11b940da3ed" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeAxis_954f3059-2660-4b49-9819-f52a27bf185e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SubsequentEventTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesTable_2bd2095b-07cd-4f52-bbae-4d117a98839b" xlink:to="loc_us-gaap_SubsequentEventTypeAxis_954f3059-2660-4b49-9819-f52a27bf185e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_954f3059-2660-4b49-9819-f52a27bf185e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_954f3059-2660-4b49-9819-f52a27bf185e" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_954f3059-2660-4b49-9819-f52a27bf185e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_e86907f1-1441-45cf-97fd-1fdf7699174c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_954f3059-2660-4b49-9819-f52a27bf185e" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_e86907f1-1441-45cf-97fd-1fdf7699174c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventMember_fd099fc8-585e-4a59-9252-beb44d94e965" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SubsequentEventMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventTypeDomain_e86907f1-1441-45cf-97fd-1fdf7699174c" xlink:to="loc_us-gaap_SubsequentEventMember_fd099fc8-585e-4a59-9252-beb44d94e965" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/PortfolioLoansSummaryofPortfolioLoansbyCategoryDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#PortfolioLoansSummaryofPortfolioLoansbyCategoryDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/PortfolioLoansSummaryofPortfolioLoansbyCategoryDetails" xlink:type="extended" id="i5901f0f4ff4a44089babb92bf87a0f34_PortfolioLoansSummaryofPortfolioLoansbyCategoryDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_aa2ab40e-f716-4f52-9862-00cac85d34c9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AccountsNotesAndLoansReceivableLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NotesReceivableGross_cfe9bd85-8271-4f88-8ef8-873aaeb3dd7a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NotesReceivableGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_aa2ab40e-f716-4f52-9862-00cac85d34c9" xlink:to="loc_us-gaap_NotesReceivableGross_cfe9bd85-8271-4f88-8ef8-873aaeb3dd7a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnamortizedLoanCommitmentAndOriginationFeesAndUnamortizedDiscountsOrPremiums_d3b035c9-7a8a-45c3-9413-eeb339ca1df7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_UnamortizedLoanCommitmentAndOriginationFeesAndUnamortizedDiscountsOrPremiums"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_aa2ab40e-f716-4f52-9862-00cac85d34c9" xlink:to="loc_us-gaap_UnamortizedLoanCommitmentAndOriginationFeesAndUnamortizedDiscountsOrPremiums_d3b035c9-7a8a-45c3-9413-eeb339ca1df7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansAndLeaseReceivableIncludingUnearnedLoanFees_bf6210ab-e646-4bb5-b4bd-00c4e481cb06" xlink:href="efsc-20221231.xsd#efsc_LoansAndLeaseReceivableIncludingUnearnedLoanFees"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_aa2ab40e-f716-4f52-9862-00cac85d34c9" xlink:to="loc_efsc_LoansAndLeaseReceivableIncludingUnearnedLoanFees_bf6210ab-e646-4bb5-b4bd-00c4e481cb06" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_c8be77f4-b5db-4a12-8ef4-8d5b6caa6d9f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_aa2ab40e-f716-4f52-9862-00cac85d34c9" xlink:to="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_c8be77f4-b5db-4a12-8ef4-8d5b6caa6d9f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansByMajorCategoryAxis_8509cb6b-22c8-42ef-8f44-706b1b319067" xlink:href="efsc-20221231.xsd#efsc_LoansByMajorCategoryAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_c8be77f4-b5db-4a12-8ef4-8d5b6caa6d9f" xlink:to="loc_efsc_LoansByMajorCategoryAxis_8509cb6b-22c8-42ef-8f44-706b1b319067" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansByMajorCategoryDomain_8509cb6b-22c8-42ef-8f44-706b1b319067_default" xlink:href="efsc-20221231.xsd#efsc_LoansByMajorCategoryDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_efsc_LoansByMajorCategoryAxis_8509cb6b-22c8-42ef-8f44-706b1b319067" xlink:to="loc_efsc_LoansByMajorCategoryDomain_8509cb6b-22c8-42ef-8f44-706b1b319067_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansByMajorCategoryDomain_38717ee3-73a4-4ee8-85b6-10e2ecd098da" xlink:href="efsc-20221231.xsd#efsc_LoansByMajorCategoryDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_efsc_LoansByMajorCategoryAxis_8509cb6b-22c8-42ef-8f44-706b1b319067" xlink:to="loc_efsc_LoansByMajorCategoryDomain_38717ee3-73a4-4ee8-85b6-10e2ecd098da" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_NoncoveredLoansMember_b87b1e30-e7e1-41e0-b911-7016a1649ce6" xlink:href="efsc-20221231.xsd#efsc_NoncoveredLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_efsc_LoansByMajorCategoryDomain_38717ee3-73a4-4ee8-85b6-10e2ecd098da" xlink:to="loc_efsc_NoncoveredLoansMember_b87b1e30-e7e1-41e0-b911-7016a1649ce6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_3d06743b-cdc2-4aa4-a38d-35be0aa56423" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_c8be77f4-b5db-4a12-8ef4-8d5b6caa6d9f" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_3d06743b-cdc2-4aa4-a38d-35be0aa56423" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_3d06743b-cdc2-4aa4-a38d-35be0aa56423_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_3d06743b-cdc2-4aa4-a38d-35be0aa56423" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_3d06743b-cdc2-4aa4-a38d-35be0aa56423_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_3519b581-5fbc-4629-b1f7-80461bd0dd6e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_3d06743b-cdc2-4aa4-a38d-35be0aa56423" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_3519b581-5fbc-4629-b1f7-80461bd0dd6e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CommercialAndIndustrialPortfolioSegmentMember_99021da9-9e1e-43ad-99b4-8b456d3e0b5a" xlink:href="efsc-20221231.xsd#efsc_CommercialAndIndustrialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_3519b581-5fbc-4629-b1f7-80461bd0dd6e" xlink:to="loc_efsc_CommercialAndIndustrialPortfolioSegmentMember_99021da9-9e1e-43ad-99b4-8b456d3e0b5a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CommercialRealEstateInvestorOwnedFinancingReceivableMember_c5ca811a-ecc2-4963-84ad-2e22085ba19e" xlink:href="efsc-20221231.xsd#efsc_CommercialRealEstateInvestorOwnedFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_3519b581-5fbc-4629-b1f7-80461bd0dd6e" xlink:to="loc_efsc_CommercialRealEstateInvestorOwnedFinancingReceivableMember_c5ca811a-ecc2-4963-84ad-2e22085ba19e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CommercialRealEstateOwnerOccupiedFinancingReceivableMember_685fe5ce-1d53-4fff-80a8-369dc63d279c" xlink:href="efsc-20221231.xsd#efsc_CommercialRealEstateOwnerOccupiedFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_3519b581-5fbc-4629-b1f7-80461bd0dd6e" xlink:to="loc_efsc_CommercialRealEstateOwnerOccupiedFinancingReceivableMember_685fe5ce-1d53-4fff-80a8-369dc63d279c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ConstructionAndLandDevelopmentFinancingReceivableMember_dc1418c2-28ef-4c55-9cf6-82971b041eb2" xlink:href="efsc-20221231.xsd#efsc_ConstructionAndLandDevelopmentFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_3519b581-5fbc-4629-b1f7-80461bd0dd6e" xlink:to="loc_efsc_ConstructionAndLandDevelopmentFinancingReceivableMember_dc1418c2-28ef-4c55-9cf6-82971b041eb2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ResidentialRealEstateFinancingReceivableMember_c7166a8c-6405-4536-940b-d8cd43f17b95" xlink:href="efsc-20221231.xsd#efsc_ResidentialRealEstateFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_3519b581-5fbc-4629-b1f7-80461bd0dd6e" xlink:to="loc_efsc_ResidentialRealEstateFinancingReceivableMember_c7166a8c-6405-4536-940b-d8cd43f17b95" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_RealEstateLoansPortfolioSegmentMember_8084d3db-dd5e-4f81-be85-39e8ee2c64ca" xlink:href="efsc-20221231.xsd#efsc_RealEstateLoansPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_3519b581-5fbc-4629-b1f7-80461bd0dd6e" xlink:to="loc_efsc_RealEstateLoansPortfolioSegmentMember_8084d3db-dd5e-4f81-be85-39e8ee2c64ca" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ConsumerAndOtherPortfolioSegmentMember_d1a6c031-cfdc-416c-be10-74ea9ce0c869" xlink:href="efsc-20221231.xsd#efsc_ConsumerAndOtherPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_3519b581-5fbc-4629-b1f7-80461bd0dd6e" xlink:to="loc_efsc_ConsumerAndOtherPortfolioSegmentMember_d1a6c031-cfdc-416c-be10-74ea9ce0c869" xlink:type="arc" order="6"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/PortfolioLoansSummaryofAllowanceforLoanLossesbyPortfolioClassandCategoryDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#PortfolioLoansSummaryofAllowanceforLoanLossesbyPortfolioClassandCategoryDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/PortfolioLoansSummaryofAllowanceforLoanLossesbyPortfolioClassandCategoryDetails" xlink:type="extended" id="ic4a57a3094ce4805b3f49705b9302484_PortfolioLoansSummaryofAllowanceforLoanLossesbyPortfolioClassandCategoryDetails">
    <link:loc xlink:type="locator" xlink:label="loc_efsc_SummaryOfActivityInAllowanceForLoanLossesByPortfolioClassAndCategoryBasedOnImpairmentMethodLineItems_2b406060-ac70-496b-944a-09857e916282" xlink:href="efsc-20221231.xsd#efsc_SummaryOfActivityInAllowanceForLoanLossesByPortfolioClassAndCategoryBasedOnImpairmentMethodLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesRollForward_e7f5471b-b2ff-4529-ad40-c64adbc03b0d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableAllowanceForCreditLossesRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_efsc_SummaryOfActivityInAllowanceForLoanLossesByPortfolioClassAndCategoryBasedOnImpairmentMethodLineItems_2b406060-ac70-496b-944a-09857e916282" xlink:to="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesRollForward_e7f5471b-b2ff-4529-ad40-c64adbc03b0d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableAllowanceForCreditLosses_65a9e1c9-d579-4e60-b215-f814b75947d5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableAllowanceForCreditLosses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesRollForward_e7f5471b-b2ff-4529-ad40-c64adbc03b0d" xlink:to="loc_us-gaap_FinancingReceivableAllowanceForCreditLosses_65a9e1c9-d579-4e60-b215-f814b75947d5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProvisionForLoanLeaseAndOtherLosses_233b3c55-094b-4a95-bc28-ea2f90ef68bd" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ProvisionForLoanLeaseAndOtherLosses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesRollForward_e7f5471b-b2ff-4529-ad40-c64adbc03b0d" xlink:to="loc_us-gaap_ProvisionForLoanLeaseAndOtherLosses_233b3c55-094b-4a95-bc28-ea2f90ef68bd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_InitialAllowanceOnAcquiredPCDLoans_9dd92dc1-50ac-4a47-9fa5-9d98c3df1a8c" xlink:href="efsc-20221231.xsd#efsc_InitialAllowanceOnAcquiredPCDLoans"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesRollForward_e7f5471b-b2ff-4529-ad40-c64adbc03b0d" xlink:to="loc_efsc_InitialAllowanceOnAcquiredPCDLoans_9dd92dc1-50ac-4a47-9fa5-9d98c3df1a8c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesWriteOffs_58c2f378-f9e6-4bb9-b04f-cd56680cdedc" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableAllowanceForCreditLossesWriteOffs"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesRollForward_e7f5471b-b2ff-4529-ad40-c64adbc03b0d" xlink:to="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesWriteOffs_58c2f378-f9e6-4bb9-b04f-cd56680cdedc" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesRecovery_24821284-e112-49b3-ba98-5e3d0cf1348e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableAllowanceForCreditLossesRecovery"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesRollForward_e7f5471b-b2ff-4529-ad40-c64adbc03b0d" xlink:to="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesRecovery_24821284-e112-49b3-ba98-5e3d0cf1348e" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableAllowanceForCreditLosses_b04fd486-9b4e-48c4-9135-90b9666f23a3" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableAllowanceForCreditLosses"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_SummaryOfActivityInAllowanceForLoanLossesByPortfolioClassAndCategoryBasedOnImpairmentMethodTable_813b89a4-2c30-4c1d-a709-d4837226367a" xlink:href="efsc-20221231.xsd#efsc_SummaryOfActivityInAllowanceForLoanLossesByPortfolioClassAndCategoryBasedOnImpairmentMethodTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_efsc_SummaryOfActivityInAllowanceForLoanLossesByPortfolioClassAndCategoryBasedOnImpairmentMethodLineItems_2b406060-ac70-496b-944a-09857e916282" xlink:to="loc_efsc_SummaryOfActivityInAllowanceForLoanLossesByPortfolioClassAndCategoryBasedOnImpairmentMethodTable_813b89a4-2c30-4c1d-a709-d4837226367a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansByMajorCategoryAxis_c8d24642-8ae2-4b35-a34f-af9f7f9da4df" xlink:href="efsc-20221231.xsd#efsc_LoansByMajorCategoryAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_efsc_SummaryOfActivityInAllowanceForLoanLossesByPortfolioClassAndCategoryBasedOnImpairmentMethodTable_813b89a4-2c30-4c1d-a709-d4837226367a" xlink:to="loc_efsc_LoansByMajorCategoryAxis_c8d24642-8ae2-4b35-a34f-af9f7f9da4df" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansByMajorCategoryDomain_c8d24642-8ae2-4b35-a34f-af9f7f9da4df_default" xlink:href="efsc-20221231.xsd#efsc_LoansByMajorCategoryDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_efsc_LoansByMajorCategoryAxis_c8d24642-8ae2-4b35-a34f-af9f7f9da4df" xlink:to="loc_efsc_LoansByMajorCategoryDomain_c8d24642-8ae2-4b35-a34f-af9f7f9da4df_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansByMajorCategoryDomain_189a7bfa-ec5b-48f4-989b-717a871110f2" xlink:href="efsc-20221231.xsd#efsc_LoansByMajorCategoryDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_efsc_LoansByMajorCategoryAxis_c8d24642-8ae2-4b35-a34f-af9f7f9da4df" xlink:to="loc_efsc_LoansByMajorCategoryDomain_189a7bfa-ec5b-48f4-989b-717a871110f2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_NoncoveredLoansMember_6dab0f91-792e-4327-a11d-1270b9e700dc" xlink:href="efsc-20221231.xsd#efsc_NoncoveredLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_efsc_LoansByMajorCategoryDomain_189a7bfa-ec5b-48f4-989b-717a871110f2" xlink:to="loc_efsc_NoncoveredLoansMember_6dab0f91-792e-4327-a11d-1270b9e700dc" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_53e1e6ff-6697-48e8-8cd4-a67f399725ab" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_efsc_SummaryOfActivityInAllowanceForLoanLossesByPortfolioClassAndCategoryBasedOnImpairmentMethodTable_813b89a4-2c30-4c1d-a709-d4837226367a" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_53e1e6ff-6697-48e8-8cd4-a67f399725ab" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_53e1e6ff-6697-48e8-8cd4-a67f399725ab_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_53e1e6ff-6697-48e8-8cd4-a67f399725ab" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_53e1e6ff-6697-48e8-8cd4-a67f399725ab_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_591de387-2df4-49ad-bda6-7d6adcdd954a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_53e1e6ff-6697-48e8-8cd4-a67f399725ab" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_591de387-2df4-49ad-bda6-7d6adcdd954a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CommercialAndIndustrialPortfolioSegmentMember_ad3a43a7-6c1b-4e2e-b2a3-6d4675f84622" xlink:href="efsc-20221231.xsd#efsc_CommercialAndIndustrialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_591de387-2df4-49ad-bda6-7d6adcdd954a" xlink:to="loc_efsc_CommercialAndIndustrialPortfolioSegmentMember_ad3a43a7-6c1b-4e2e-b2a3-6d4675f84622" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CommercialRealEstateInvestorOwnedFinancingReceivableMember_311a0d36-e5df-4095-aaa9-cbcfdf452808" xlink:href="efsc-20221231.xsd#efsc_CommercialRealEstateInvestorOwnedFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_591de387-2df4-49ad-bda6-7d6adcdd954a" xlink:to="loc_efsc_CommercialRealEstateInvestorOwnedFinancingReceivableMember_311a0d36-e5df-4095-aaa9-cbcfdf452808" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CommercialRealEstateOwnerOccupiedFinancingReceivableMember_0420f016-09c3-4315-b693-ab56984b534c" xlink:href="efsc-20221231.xsd#efsc_CommercialRealEstateOwnerOccupiedFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_591de387-2df4-49ad-bda6-7d6adcdd954a" xlink:to="loc_efsc_CommercialRealEstateOwnerOccupiedFinancingReceivableMember_0420f016-09c3-4315-b693-ab56984b534c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ConstructionAndLandDevelopmentFinancingReceivableMember_01aa6558-82c4-4721-a8ae-c68821e74797" xlink:href="efsc-20221231.xsd#efsc_ConstructionAndLandDevelopmentFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_591de387-2df4-49ad-bda6-7d6adcdd954a" xlink:to="loc_efsc_ConstructionAndLandDevelopmentFinancingReceivableMember_01aa6558-82c4-4721-a8ae-c68821e74797" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ResidentialRealEstateFinancingReceivableMember_d1dc39fc-eef3-4943-9fdd-7001307ad4eb" xlink:href="efsc-20221231.xsd#efsc_ResidentialRealEstateFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_591de387-2df4-49ad-bda6-7d6adcdd954a" xlink:to="loc_efsc_ResidentialRealEstateFinancingReceivableMember_d1dc39fc-eef3-4943-9fdd-7001307ad4eb" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ConsumerAndOtherPortfolioSegmentMember_f5cfb171-9296-43db-93c4-2bd3710a0514" xlink:href="efsc-20221231.xsd#efsc_ConsumerAndOtherPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_591de387-2df4-49ad-bda6-7d6adcdd954a" xlink:to="loc_efsc_ConsumerAndOtherPortfolioSegmentMember_f5cfb171-9296-43db-93c4-2bd3710a0514" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/PortfolioLoansCollateralDependentLoansDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#PortfolioLoansCollateralDependentLoansDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/PortfolioLoansCollateralDependentLoansDetails" xlink:type="extended" id="i148c671aa4ae43249a3f311f6e4cea28_PortfolioLoansCollateralDependentLoansDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableNonaccrualStatusLineItems_36e81c89-b64c-41f6-a720-cbba98000b9b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableNonaccrualStatusLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentNonaccrualStatus_09c0c134-c520-4a77-bb3c-2128a7292172" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentNonaccrualStatus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableNonaccrualStatusLineItems_36e81c89-b64c-41f6-a720-cbba98000b9b" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentNonaccrualStatus_09c0c134-c520-4a77-bb3c-2128a7292172" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableNonaccrualTable_8e880167-0149-42db-9166-339501901628" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableNonaccrualTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FinancingReceivableNonaccrualStatusLineItems_36e81c89-b64c-41f6-a720-cbba98000b9b" xlink:to="loc_us-gaap_FinancingReceivableNonaccrualTable_8e880167-0149-42db-9166-339501901628" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CollateralAxis_ff1ee506-2301-4ea6-af73-526d5936da52" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CollateralAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FinancingReceivableNonaccrualTable_8e880167-0149-42db-9166-339501901628" xlink:to="loc_us-gaap_CollateralAxis_ff1ee506-2301-4ea6-af73-526d5936da52" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CollateralDomain_ff1ee506-2301-4ea6-af73-526d5936da52_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CollateralDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CollateralAxis_ff1ee506-2301-4ea6-af73-526d5936da52" xlink:to="loc_us-gaap_CollateralDomain_ff1ee506-2301-4ea6-af73-526d5936da52_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CollateralDomain_21e318bf-4a12-43a2-82b0-8f4dbc339ba1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CollateralDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CollateralAxis_ff1ee506-2301-4ea6-af73-526d5936da52" xlink:to="loc_us-gaap_CollateralDomain_21e318bf-4a12-43a2-82b0-8f4dbc339ba1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialRealEstateMember_abda900f-88e5-4109-b169-af90038116a0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommercialRealEstateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollateralDomain_21e318bf-4a12-43a2-82b0-8f4dbc339ba1" xlink:to="loc_us-gaap_CommercialRealEstateMember_abda900f-88e5-4109-b169-af90038116a0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResidentialRealEstateMember_13c254ff-ab17-4595-af62-35ec9522d5d5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ResidentialRealEstateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollateralDomain_21e318bf-4a12-43a2-82b0-8f4dbc339ba1" xlink:to="loc_us-gaap_ResidentialRealEstateMember_13c254ff-ab17-4595-af62-35ec9522d5d5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_BlanketLienMember_575a3a29-131e-4e94-b207-c692b26cc4dc" xlink:href="efsc-20221231.xsd#efsc_BlanketLienMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollateralDomain_21e318bf-4a12-43a2-82b0-8f4dbc339ba1" xlink:to="loc_efsc_BlanketLienMember_575a3a29-131e-4e94-b207-c692b26cc4dc" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_bb3930ac-327b-432d-a282-2cb768d7df7c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivablePortfolioSegmentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FinancingReceivableNonaccrualTable_8e880167-0149-42db-9166-339501901628" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_bb3930ac-327b-432d-a282-2cb768d7df7c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_bb3930ac-327b-432d-a282-2cb768d7df7c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_bb3930ac-327b-432d-a282-2cb768d7df7c" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_bb3930ac-327b-432d-a282-2cb768d7df7c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_8f39e8a5-6030-418b-b0a0-460b745dae5b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_bb3930ac-327b-432d-a282-2cb768d7df7c" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_8f39e8a5-6030-418b-b0a0-460b745dae5b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CommercialAndIndustrialPortfolioSegmentMember_0bbbfb60-2d31-4dfe-8850-e334507ea93d" xlink:href="efsc-20221231.xsd#efsc_CommercialAndIndustrialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_8f39e8a5-6030-418b-b0a0-460b745dae5b" xlink:to="loc_efsc_CommercialAndIndustrialPortfolioSegmentMember_0bbbfb60-2d31-4dfe-8850-e334507ea93d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialPortfolioSegmentMember_0c007987-e2c5-4f12-b6f3-d9b3b6095a16" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommercialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_8f39e8a5-6030-418b-b0a0-460b745dae5b" xlink:to="loc_us-gaap_CommercialPortfolioSegmentMember_0c007987-e2c5-4f12-b6f3-d9b3b6095a16" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResidentialPortfolioSegmentMember_9263da45-7021-4990-8125-79e71295db11" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ResidentialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_8f39e8a5-6030-418b-b0a0-460b745dae5b" xlink:to="loc_us-gaap_ResidentialPortfolioSegmentMember_9263da45-7021-4990-8125-79e71295db11" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnallocatedFinancingReceivablesMember_c95919f7-7bb3-4779-8e69-f07bac6a9aee" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_UnallocatedFinancingReceivablesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_8f39e8a5-6030-418b-b0a0-460b745dae5b" xlink:to="loc_us-gaap_UnallocatedFinancingReceivablesMember_c95919f7-7bb3-4779-8e69-f07bac6a9aee" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_9a3516cb-37e5-4b4d-92a2-6e8c52a0d851" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FinancingReceivableNonaccrualTable_8e880167-0149-42db-9166-339501901628" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_9a3516cb-37e5-4b4d-92a2-6e8c52a0d851" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_9a3516cb-37e5-4b4d-92a2-6e8c52a0d851_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_9a3516cb-37e5-4b4d-92a2-6e8c52a0d851" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_9a3516cb-37e5-4b4d-92a2-6e8c52a0d851_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_13949b68-327e-4c6b-aeb8-d518b89dbc99" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_9a3516cb-37e5-4b4d-92a2-6e8c52a0d851" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_13949b68-327e-4c6b-aeb8-d518b89dbc99" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CommercialAndIndustrialPortfolioSegmentMember_0857c80a-8a3b-4059-bb3b-c6a870439159" xlink:href="efsc-20221231.xsd#efsc_CommercialAndIndustrialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_13949b68-327e-4c6b-aeb8-d518b89dbc99" xlink:to="loc_efsc_CommercialAndIndustrialPortfolioSegmentMember_0857c80a-8a3b-4059-bb3b-c6a870439159" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CommercialRealEstateInvestorOwnedFinancingReceivableMember_8b65bd67-236c-4c8b-bc08-21de4ec2400f" xlink:href="efsc-20221231.xsd#efsc_CommercialRealEstateInvestorOwnedFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_13949b68-327e-4c6b-aeb8-d518b89dbc99" xlink:to="loc_efsc_CommercialRealEstateInvestorOwnedFinancingReceivableMember_8b65bd67-236c-4c8b-bc08-21de4ec2400f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CommercialRealEstateOwnerOccupiedFinancingReceivableMember_03abd8cf-5f9a-4d2a-a75d-88a8d61abcce" xlink:href="efsc-20221231.xsd#efsc_CommercialRealEstateOwnerOccupiedFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_13949b68-327e-4c6b-aeb8-d518b89dbc99" xlink:to="loc_efsc_CommercialRealEstateOwnerOccupiedFinancingReceivableMember_03abd8cf-5f9a-4d2a-a75d-88a8d61abcce" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ConstructionAndLandDevelopmentFinancingReceivableMember_704b819b-a508-4941-b81b-ed907af93312" xlink:href="efsc-20221231.xsd#efsc_ConstructionAndLandDevelopmentFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_13949b68-327e-4c6b-aeb8-d518b89dbc99" xlink:to="loc_efsc_ConstructionAndLandDevelopmentFinancingReceivableMember_704b819b-a508-4941-b81b-ed907af93312" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ResidentialRealEstateFinancingReceivableMember_083535f7-9dde-4f47-be09-17458295b613" xlink:href="efsc-20221231.xsd#efsc_ResidentialRealEstateFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_13949b68-327e-4c6b-aeb8-d518b89dbc99" xlink:to="loc_efsc_ResidentialRealEstateFinancingReceivableMember_083535f7-9dde-4f47-be09-17458295b613" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ConsumerAndOtherPortfolioSegmentMember_c270cc16-cd78-4237-b73e-c0147d25314b" xlink:href="efsc-20221231.xsd#efsc_ConsumerAndOtherPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_13949b68-327e-4c6b-aeb8-d518b89dbc99" xlink:to="loc_efsc_ConsumerAndOtherPortfolioSegmentMember_c270cc16-cd78-4237-b73e-c0147d25314b" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/PortfolioLoansNarrativeDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#PortfolioLoansNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/PortfolioLoansNarrativeDetails" xlink:type="extended" id="i96bf493b777549c0803c55495ab39a32_PortfolioLoansNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d5ef2005-a413-444f-b27e-190b4082d050" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AccountsNotesAndLoansReceivableLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansAndLeasesReceivableImpairedLoansCommitmentToLendReserve_0072185b-790d-4016-ac4d-5f56000397fd" xlink:href="efsc-20221231.xsd#efsc_LoansAndLeasesReceivableImpairedLoansCommitmentToLendReserve"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d5ef2005-a413-444f-b27e-190b4082d050" xlink:to="loc_efsc_LoansAndLeasesReceivableImpairedLoansCommitmentToLendReserve_0072185b-790d-4016-ac4d-5f56000397fd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_FinancingReceivablePaycheckProtectionProgram_503060bd-391d-4f74-8379-32fd1bba848e" xlink:href="efsc-20221231.xsd#efsc_FinancingReceivablePaycheckProtectionProgram"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d5ef2005-a413-444f-b27e-190b4082d050" xlink:to="loc_efsc_FinancingReceivablePaycheckProtectionProgram_503060bd-391d-4f74-8379-32fd1bba848e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_FinancingReceivablePaycheckProtectionProgramNetOfDeferredFees_f36575f2-6f51-44aa-a9b0-3577d577ef88" xlink:href="efsc-20221231.xsd#efsc_FinancingReceivablePaycheckProtectionProgramNetOfDeferredFees"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d5ef2005-a413-444f-b27e-190b4082d050" xlink:to="loc_efsc_FinancingReceivablePaycheckProtectionProgramNetOfDeferredFees_f36575f2-6f51-44aa-a9b0-3577d577ef88" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_FinancingRecievablePaycheckProtectionProgramUnearnedFees_5f8cf068-fdd2-4df1-8658-f43aa2d11734" xlink:href="efsc-20221231.xsd#efsc_FinancingRecievablePaycheckProtectionProgramUnearnedFees"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d5ef2005-a413-444f-b27e-190b4082d050" xlink:to="loc_efsc_FinancingRecievablePaycheckProtectionProgramUnearnedFees_5f8cf068-fdd2-4df1-8658-f43aa2d11734" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FederalHomeLoanBankAdvancesGeneralDebtObligationsDisclosuresMaximumAmountAvailable_3b684af8-0eb4-478e-bc20-f551e8d56b81" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FederalHomeLoanBankAdvancesGeneralDebtObligationsDisclosuresMaximumAmountAvailable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d5ef2005-a413-444f-b27e-190b4082d050" xlink:to="loc_us-gaap_FederalHomeLoanBankAdvancesGeneralDebtObligationsDisclosuresMaximumAmountAvailable_3b684af8-0eb4-478e-bc20-f551e8d56b81" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_NetPremiumAcquiredLoans_ab807e40-7158-47e0-a1b2-87876d72918f" xlink:href="efsc-20221231.xsd#efsc_NetPremiumAcquiredLoans"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d5ef2005-a413-444f-b27e-190b4082d050" xlink:to="loc_efsc_NetPremiumAcquiredLoans_ab807e40-7158-47e0-a1b2-87876d72918f" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProvisionForLoanLossesExpensed_f08b87e1-b42a-4977-9102-35e43f6d548f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ProvisionForLoanLossesExpensed"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d5ef2005-a413-444f-b27e-190b4082d050" xlink:to="loc_us-gaap_ProvisionForLoanLossesExpensed_f08b87e1-b42a-4977-9102-35e43f6d548f" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_AllowanceForCreditLossesQualitativeAdjustment_76b062a7-8e0b-45ff-8f21-7dc713e6ba0f" xlink:href="efsc-20221231.xsd#efsc_AllowanceForCreditLossesQualitativeAdjustment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d5ef2005-a413-444f-b27e-190b4082d050" xlink:to="loc_efsc_AllowanceForCreditLossesQualitativeAdjustment_76b062a7-8e0b-45ff-8f21-7dc713e6ba0f" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableModificationsNumberOfContracts2_6cdd27d3-c999-4a07-af4c-1edd13db9a1a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableModificationsNumberOfContracts2"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d5ef2005-a413-444f-b27e-190b4082d050" xlink:to="loc_us-gaap_FinancingReceivableModificationsNumberOfContracts2_6cdd27d3-c999-4a07-af4c-1edd13db9a1a" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableModificationsSubsequentDefaultNumberOfContracts1_e814f51a-5fcf-4b37-b1f9-ffea47cb888d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableModificationsSubsequentDefaultNumberOfContracts1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d5ef2005-a413-444f-b27e-190b4082d050" xlink:to="loc_us-gaap_FinancingReceivableModificationsSubsequentDefaultNumberOfContracts1_e814f51a-5fcf-4b37-b1f9-ffea47cb888d" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableAllowanceForCreditLosses_f61ec143-e2d1-4414-bb55-907fbded152e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableAllowanceForCreditLosses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d5ef2005-a413-444f-b27e-190b4082d050" xlink:to="loc_us-gaap_FinancingReceivableAllowanceForCreditLosses_f61ec143-e2d1-4414-bb55-907fbded152e" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NotesReceivableRelatedParties_1da58cec-3d84-44f8-a32b-7424ac6f495c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NotesReceivableRelatedParties"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d5ef2005-a413-444f-b27e-190b4082d050" xlink:to="loc_us-gaap_NotesReceivableRelatedParties_1da58cec-3d84-44f8-a32b-7424ac6f495c" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_FinancingReceivableCreditLossExpenseReversalAdditionalProvision_c1ee2188-5489-4272-b976-3f37aed44ee6" xlink:href="efsc-20221231.xsd#efsc_FinancingReceivableCreditLossExpenseReversalAdditionalProvision"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d5ef2005-a413-444f-b27e-190b4082d050" xlink:to="loc_efsc_FinancingReceivableCreditLossExpenseReversalAdditionalProvision_c1ee2188-5489-4272-b976-3f37aed44ee6" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_FinancingReceivableCreditLossExpenseReversalAcquisitionRelatedExpense_a05d8826-47a6-495f-8168-a3c18462dccc" xlink:href="efsc-20221231.xsd#efsc_FinancingReceivableCreditLossExpenseReversalAcquisitionRelatedExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d5ef2005-a413-444f-b27e-190b4082d050" xlink:to="loc_efsc_FinancingReceivableCreditLossExpenseReversalAcquisitionRelatedExpense_a05d8826-47a6-495f-8168-a3c18462dccc" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_a2890c47-9ca3-4483-bfc0-037274dc7135" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_d5ef2005-a413-444f-b27e-190b4082d050" xlink:to="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_a2890c47-9ca3-4483-bfc0-037274dc7135" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_NatureOfCommitmentsAxis_b30ba52d-2538-44c3-9af3-9057919bc6bb" xlink:href="efsc-20221231.xsd#efsc_NatureOfCommitmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_a2890c47-9ca3-4483-bfc0-037274dc7135" xlink:to="loc_efsc_NatureOfCommitmentsAxis_b30ba52d-2538-44c3-9af3-9057919bc6bb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_NatureOfCommitmentsDomain_b30ba52d-2538-44c3-9af3-9057919bc6bb_default" xlink:href="efsc-20221231.xsd#efsc_NatureOfCommitmentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_efsc_NatureOfCommitmentsAxis_b30ba52d-2538-44c3-9af3-9057919bc6bb" xlink:to="loc_efsc_NatureOfCommitmentsDomain_b30ba52d-2538-44c3-9af3-9057919bc6bb_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_NatureOfCommitmentsDomain_9bae9bca-7a0d-44fa-947f-008d23d2a973" xlink:href="efsc-20221231.xsd#efsc_NatureOfCommitmentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_efsc_NatureOfCommitmentsAxis_b30ba52d-2538-44c3-9af3-9057919bc6bb" xlink:to="loc_efsc_NatureOfCommitmentsDomain_9bae9bca-7a0d-44fa-947f-008d23d2a973" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_UnadvancedCommitmentOnImpairedLoanMember_c3de0eec-390b-4c20-be61-aa33780b646e" xlink:href="efsc-20221231.xsd#efsc_UnadvancedCommitmentOnImpairedLoanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_efsc_NatureOfCommitmentsDomain_9bae9bca-7a0d-44fa-947f-008d23d2a973" xlink:to="loc_efsc_UnadvancedCommitmentOnImpairedLoanMember_c3de0eec-390b-4c20-be61-aa33780b646e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansByMajorCategoryAxis_dd12f03d-b26f-4bf2-9c00-f7a9c357fa5c" xlink:href="efsc-20221231.xsd#efsc_LoansByMajorCategoryAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_a2890c47-9ca3-4483-bfc0-037274dc7135" xlink:to="loc_efsc_LoansByMajorCategoryAxis_dd12f03d-b26f-4bf2-9c00-f7a9c357fa5c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansByMajorCategoryDomain_dd12f03d-b26f-4bf2-9c00-f7a9c357fa5c_default" xlink:href="efsc-20221231.xsd#efsc_LoansByMajorCategoryDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_efsc_LoansByMajorCategoryAxis_dd12f03d-b26f-4bf2-9c00-f7a9c357fa5c" xlink:to="loc_efsc_LoansByMajorCategoryDomain_dd12f03d-b26f-4bf2-9c00-f7a9c357fa5c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansByMajorCategoryDomain_9bcf81f4-8c92-4886-8e6c-ac66c99bbce8" xlink:href="efsc-20221231.xsd#efsc_LoansByMajorCategoryDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_efsc_LoansByMajorCategoryAxis_dd12f03d-b26f-4bf2-9c00-f7a9c357fa5c" xlink:to="loc_efsc_LoansByMajorCategoryDomain_9bcf81f4-8c92-4886-8e6c-ac66c99bbce8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_NoncoveredLoansMember_1b5f1cf0-3a29-4dd5-a865-658697561cc0" xlink:href="efsc-20221231.xsd#efsc_NoncoveredLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_efsc_LoansByMajorCategoryDomain_9bcf81f4-8c92-4886-8e6c-ac66c99bbce8" xlink:to="loc_efsc_NoncoveredLoansMember_1b5f1cf0-3a29-4dd5-a865-658697561cc0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_29f3b082-50b0-41a7-baea-dd7596c8d488" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_a2890c47-9ca3-4483-bfc0-037274dc7135" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_29f3b082-50b0-41a7-baea-dd7596c8d488" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_29f3b082-50b0-41a7-baea-dd7596c8d488_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_29f3b082-50b0-41a7-baea-dd7596c8d488" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_29f3b082-50b0-41a7-baea-dd7596c8d488_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_7d526f62-6a14-4ab4-a294-0fb66639fef3" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_29f3b082-50b0-41a7-baea-dd7596c8d488" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_7d526f62-6a14-4ab4-a294-0fb66639fef3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_EnterpriseValueLendingPortfolioNicheSegmentMember_0e97dc7e-c63b-4733-b6db-09e912e1088d" xlink:href="efsc-20221231.xsd#efsc_EnterpriseValueLendingPortfolioNicheSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_7d526f62-6a14-4ab4-a294-0fb66639fef3" xlink:to="loc_efsc_EnterpriseValueLendingPortfolioNicheSegmentMember_0e97dc7e-c63b-4733-b6db-09e912e1088d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CumulativeEffectPeriodOfAdoptionAxis_025d4e12-1958-44e3-a701-9bce37dc77ab" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_CumulativeEffectPeriodOfAdoptionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_a2890c47-9ca3-4483-bfc0-037274dc7135" xlink:to="loc_srt_CumulativeEffectPeriodOfAdoptionAxis_025d4e12-1958-44e3-a701-9bce37dc77ab" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_025d4e12-1958-44e3-a701-9bce37dc77ab_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_CumulativeEffectPeriodOfAdoptionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CumulativeEffectPeriodOfAdoptionAxis_025d4e12-1958-44e3-a701-9bce37dc77ab" xlink:to="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_025d4e12-1958-44e3-a701-9bce37dc77ab_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_3c032a89-2dca-45da-aa6d-89c5e6f4d823" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_CumulativeEffectPeriodOfAdoptionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CumulativeEffectPeriodOfAdoptionAxis_025d4e12-1958-44e3-a701-9bce37dc77ab" xlink:to="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_3c032a89-2dca-45da-aa6d-89c5e6f4d823" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CumulativeEffectPeriodOfAdoptionAdjustmentMember_4f36281d-3061-441c-baa0-97d465636ce1" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_CumulativeEffectPeriodOfAdoptionAdjustmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_3c032a89-2dca-45da-aa6d-89c5e6f4d823" xlink:to="loc_srt_CumulativeEffectPeriodOfAdoptionAdjustmentMember_4f36281d-3061-441c-baa0-97d465636ce1" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/PortfolioLoansSummaryofRecordedInvestmentinImpairedPortfolioLoansbyCategoryDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#PortfolioLoansSummaryofRecordedInvestmentinImpairedPortfolioLoansbyCategoryDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/PortfolioLoansSummaryofRecordedInvestmentinImpairedPortfolioLoansbyCategoryDetails" xlink:type="extended" id="i4eb53ee7f6ba4980a019b45b7dd29616_PortfolioLoansSummaryofRecordedInvestmentinImpairedPortfolioLoansbyCategoryDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentPastDueLineItems_8c61a5e1-5400-4d0f-b1cb-1db0ab3b789e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentPastDueLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentNonaccrualStatus_d8c1be80-9ee2-4dec-9f43-b2f5b21c68e0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentNonaccrualStatus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentPastDueLineItems_8c61a5e1-5400-4d0f-b1cb-1db0ab3b789e" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentNonaccrualStatus_d8c1be80-9ee2-4dec-9f43-b2f5b21c68e0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableModificationsRecordedInvestment_ec5b4a16-971b-4c69-82bb-4236e51fb008" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableModificationsRecordedInvestment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentPastDueLineItems_8c61a5e1-5400-4d0f-b1cb-1db0ab3b789e" xlink:to="loc_us-gaap_FinancingReceivableModificationsRecordedInvestment_ec5b4a16-971b-4c69-82bb-4236e51fb008" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestment90DaysPastDueAndStillAccruing_c390506f-3790-4ce8-b488-a53ae9c4fb79" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestment90DaysPastDueAndStillAccruing"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentPastDueLineItems_8c61a5e1-5400-4d0f-b1cb-1db0ab3b789e" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestment90DaysPastDueAndStillAccruing_c390506f-3790-4ce8-b488-a53ae9c4fb79" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ImpairedFinancingReceivableRecordedInvestment_8d7bc2ed-47fa-4eae-ab06-9be53093bae2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ImpairedFinancingReceivableRecordedInvestment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentPastDueLineItems_8c61a5e1-5400-4d0f-b1cb-1db0ab3b789e" xlink:to="loc_us-gaap_ImpairedFinancingReceivableRecordedInvestment_8d7bc2ed-47fa-4eae-ab06-9be53093bae2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ImpairedFinancingReceivableWithNoRelatedAllowanceRecordedInvestment_ede10467-5ce4-45a1-8444-e536df4516cb" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ImpairedFinancingReceivableWithNoRelatedAllowanceRecordedInvestment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentPastDueLineItems_8c61a5e1-5400-4d0f-b1cb-1db0ab3b789e" xlink:to="loc_us-gaap_ImpairedFinancingReceivableWithNoRelatedAllowanceRecordedInvestment_ede10467-5ce4-45a1-8444-e536df4516cb" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_SummaryOfRecordedInvestmentInImpairedNonCoveredLoansByCategoryTable_a2ffc742-f286-4c7c-9ccb-ee64e21621d2" xlink:href="efsc-20221231.xsd#efsc_SummaryOfRecordedInvestmentInImpairedNonCoveredLoansByCategoryTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentPastDueLineItems_8c61a5e1-5400-4d0f-b1cb-1db0ab3b789e" xlink:to="loc_efsc_SummaryOfRecordedInvestmentInImpairedNonCoveredLoansByCategoryTable_a2ffc742-f286-4c7c-9ccb-ee64e21621d2" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansByMajorCategoryAxis_b00f5719-fd29-4bff-ba8d-6a1d36553839" xlink:href="efsc-20221231.xsd#efsc_LoansByMajorCategoryAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_efsc_SummaryOfRecordedInvestmentInImpairedNonCoveredLoansByCategoryTable_a2ffc742-f286-4c7c-9ccb-ee64e21621d2" xlink:to="loc_efsc_LoansByMajorCategoryAxis_b00f5719-fd29-4bff-ba8d-6a1d36553839" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansByMajorCategoryDomain_b00f5719-fd29-4bff-ba8d-6a1d36553839_default" xlink:href="efsc-20221231.xsd#efsc_LoansByMajorCategoryDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_efsc_LoansByMajorCategoryAxis_b00f5719-fd29-4bff-ba8d-6a1d36553839" xlink:to="loc_efsc_LoansByMajorCategoryDomain_b00f5719-fd29-4bff-ba8d-6a1d36553839_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansByMajorCategoryDomain_2de83d11-c954-4af3-84d8-575e27987fb8" xlink:href="efsc-20221231.xsd#efsc_LoansByMajorCategoryDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_efsc_LoansByMajorCategoryAxis_b00f5719-fd29-4bff-ba8d-6a1d36553839" xlink:to="loc_efsc_LoansByMajorCategoryDomain_2de83d11-c954-4af3-84d8-575e27987fb8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_NoncoveredLoansMember_8896144d-58b7-4558-8a6e-f26deaa0ba5e" xlink:href="efsc-20221231.xsd#efsc_NoncoveredLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_efsc_LoansByMajorCategoryDomain_2de83d11-c954-4af3-84d8-575e27987fb8" xlink:to="loc_efsc_NoncoveredLoansMember_8896144d-58b7-4558-8a6e-f26deaa0ba5e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_20e3d0ac-aa61-4d75-a954-277d12df98f0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_efsc_SummaryOfRecordedInvestmentInImpairedNonCoveredLoansByCategoryTable_a2ffc742-f286-4c7c-9ccb-ee64e21621d2" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_20e3d0ac-aa61-4d75-a954-277d12df98f0" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_20e3d0ac-aa61-4d75-a954-277d12df98f0_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_20e3d0ac-aa61-4d75-a954-277d12df98f0" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_20e3d0ac-aa61-4d75-a954-277d12df98f0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_b5a469b8-6a8b-43ed-95a6-9a04de12dba2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_20e3d0ac-aa61-4d75-a954-277d12df98f0" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_b5a469b8-6a8b-43ed-95a6-9a04de12dba2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CommercialAndIndustrialPortfolioSegmentMember_3d60980f-f840-4ca8-84ef-523f1a054663" xlink:href="efsc-20221231.xsd#efsc_CommercialAndIndustrialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_b5a469b8-6a8b-43ed-95a6-9a04de12dba2" xlink:to="loc_efsc_CommercialAndIndustrialPortfolioSegmentMember_3d60980f-f840-4ca8-84ef-523f1a054663" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CommercialRealEstateInvestorOwnedFinancingReceivableMember_a691fb56-f162-4198-a218-f48fee2ae06a" xlink:href="efsc-20221231.xsd#efsc_CommercialRealEstateInvestorOwnedFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_b5a469b8-6a8b-43ed-95a6-9a04de12dba2" xlink:to="loc_efsc_CommercialRealEstateInvestorOwnedFinancingReceivableMember_a691fb56-f162-4198-a218-f48fee2ae06a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CommercialRealEstateOwnerOccupiedFinancingReceivableMember_40c395e5-f18d-45b9-8829-fc311a0da916" xlink:href="efsc-20221231.xsd#efsc_CommercialRealEstateOwnerOccupiedFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_b5a469b8-6a8b-43ed-95a6-9a04de12dba2" xlink:to="loc_efsc_CommercialRealEstateOwnerOccupiedFinancingReceivableMember_40c395e5-f18d-45b9-8829-fc311a0da916" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ConstructionAndLandDevelopmentFinancingReceivableMember_3504bc65-df7a-4cab-9d49-b12a945297d4" xlink:href="efsc-20221231.xsd#efsc_ConstructionAndLandDevelopmentFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_b5a469b8-6a8b-43ed-95a6-9a04de12dba2" xlink:to="loc_efsc_ConstructionAndLandDevelopmentFinancingReceivableMember_3504bc65-df7a-4cab-9d49-b12a945297d4" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ResidentialRealEstateFinancingReceivableMember_a2ccf9c1-4a8c-4711-8a44-e5592902402c" xlink:href="efsc-20221231.xsd#efsc_ResidentialRealEstateFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_b5a469b8-6a8b-43ed-95a6-9a04de12dba2" xlink:to="loc_efsc_ResidentialRealEstateFinancingReceivableMember_a2ccf9c1-4a8c-4711-8a44-e5592902402c" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ConsumerAndOtherPortfolioSegmentMember_1ee01727-17e4-4319-9d64-f63e072f8510" xlink:href="efsc-20221231.xsd#efsc_ConsumerAndOtherPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_b5a469b8-6a8b-43ed-95a6-9a04de12dba2" xlink:to="loc_efsc_ConsumerAndOtherPortfolioSegmentMember_1ee01727-17e4-4319-9d64-f63e072f8510" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_f78c6e5f-865d-4b8a-a2d9-8823ab52a25a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivablePortfolioSegmentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_efsc_SummaryOfRecordedInvestmentInImpairedNonCoveredLoansByCategoryTable_a2ffc742-f286-4c7c-9ccb-ee64e21621d2" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_f78c6e5f-865d-4b8a-a2d9-8823ab52a25a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_f78c6e5f-865d-4b8a-a2d9-8823ab52a25a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_f78c6e5f-865d-4b8a-a2d9-8823ab52a25a" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_f78c6e5f-865d-4b8a-a2d9-8823ab52a25a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_9565619f-0aa8-40e1-ba68-fbf22fe0c388" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_f78c6e5f-865d-4b8a-a2d9-8823ab52a25a" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_9565619f-0aa8-40e1-ba68-fbf22fe0c388" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CommercialAndIndustrialPortfolioSegmentMember_78c7f9e0-4658-42ec-9ed4-0ebc537d8f18" xlink:href="efsc-20221231.xsd#efsc_CommercialAndIndustrialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_9565619f-0aa8-40e1-ba68-fbf22fe0c388" xlink:to="loc_efsc_CommercialAndIndustrialPortfolioSegmentMember_78c7f9e0-4658-42ec-9ed4-0ebc537d8f18" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/PortfolioLoansSummaryofRecordedInvestmentbyCategoryforPortfolioLoansRestructuredDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#PortfolioLoansSummaryofRecordedInvestmentbyCategoryforPortfolioLoansRestructuredDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/PortfolioLoansSummaryofRecordedInvestmentbyCategoryforPortfolioLoansRestructuredDetails" xlink:type="extended" id="i6cead39d29824bf3aa67170f4ef44522_PortfolioLoansSummaryofRecordedInvestmentbyCategoryforPortfolioLoansRestructuredDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableModificationsLineItems_744c985c-1c27-4f5e-a19d-18f6244f083e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableModificationsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentNonaccrualStatus_212e3c42-1949-4b7e-8218-6557fdf62c38" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentNonaccrualStatus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableModificationsLineItems_744c985c-1c27-4f5e-a19d-18f6244f083e" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentNonaccrualStatus_212e3c42-1949-4b7e-8218-6557fdf62c38" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableModificationsNumberOfContracts2_f24e5f98-13b4-46e0-9525-be505b29e169" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableModificationsNumberOfContracts2"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableModificationsLineItems_744c985c-1c27-4f5e-a19d-18f6244f083e" xlink:to="loc_us-gaap_FinancingReceivableModificationsNumberOfContracts2_f24e5f98-13b4-46e0-9525-be505b29e169" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableModificationsPreModificationRecordedInvestment2_98d1fc01-852c-4848-b71b-809ee8802a11" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableModificationsPreModificationRecordedInvestment2"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableModificationsLineItems_744c985c-1c27-4f5e-a19d-18f6244f083e" xlink:to="loc_us-gaap_FinancingReceivableModificationsPreModificationRecordedInvestment2_98d1fc01-852c-4848-b71b-809ee8802a11" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableModificationsPostModificationRecordedInvestment2_8e0972d7-2b7c-42e1-9ce3-e886d7822666" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableModificationsPostModificationRecordedInvestment2"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableModificationsLineItems_744c985c-1c27-4f5e-a19d-18f6244f083e" xlink:to="loc_us-gaap_FinancingReceivableModificationsPostModificationRecordedInvestment2_8e0972d7-2b7c-42e1-9ce3-e886d7822666" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFinancingReceivableTroubledDebtRestructuringsTable_93cc3cf2-8bba-4b82-828e-61ed189ef0c4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfFinancingReceivableTroubledDebtRestructuringsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FinancingReceivableModificationsLineItems_744c985c-1c27-4f5e-a19d-18f6244f083e" xlink:to="loc_us-gaap_ScheduleOfFinancingReceivableTroubledDebtRestructuringsTable_93cc3cf2-8bba-4b82-828e-61ed189ef0c4" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansByMajorCategoryAxis_b8f1caa2-a8c0-48a5-956f-ca772cc86275" xlink:href="efsc-20221231.xsd#efsc_LoansByMajorCategoryAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableTroubledDebtRestructuringsTable_93cc3cf2-8bba-4b82-828e-61ed189ef0c4" xlink:to="loc_efsc_LoansByMajorCategoryAxis_b8f1caa2-a8c0-48a5-956f-ca772cc86275" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansByMajorCategoryDomain_b8f1caa2-a8c0-48a5-956f-ca772cc86275_default" xlink:href="efsc-20221231.xsd#efsc_LoansByMajorCategoryDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_efsc_LoansByMajorCategoryAxis_b8f1caa2-a8c0-48a5-956f-ca772cc86275" xlink:to="loc_efsc_LoansByMajorCategoryDomain_b8f1caa2-a8c0-48a5-956f-ca772cc86275_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansByMajorCategoryDomain_a685861f-4e2d-4c9a-989c-1d5afc1fe8fe" xlink:href="efsc-20221231.xsd#efsc_LoansByMajorCategoryDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_efsc_LoansByMajorCategoryAxis_b8f1caa2-a8c0-48a5-956f-ca772cc86275" xlink:to="loc_efsc_LoansByMajorCategoryDomain_a685861f-4e2d-4c9a-989c-1d5afc1fe8fe" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_NoncoveredLoansMember_20a4b96f-f8c7-4a89-b856-88e3742b3e35" xlink:href="efsc-20221231.xsd#efsc_NoncoveredLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_efsc_LoansByMajorCategoryDomain_a685861f-4e2d-4c9a-989c-1d5afc1fe8fe" xlink:to="loc_efsc_NoncoveredLoansMember_20a4b96f-f8c7-4a89-b856-88e3742b3e35" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_5c4e576d-8b52-4ce8-8c6d-b3c1d84284e4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableTroubledDebtRestructuringsTable_93cc3cf2-8bba-4b82-828e-61ed189ef0c4" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_5c4e576d-8b52-4ce8-8c6d-b3c1d84284e4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_5c4e576d-8b52-4ce8-8c6d-b3c1d84284e4_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_5c4e576d-8b52-4ce8-8c6d-b3c1d84284e4" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_5c4e576d-8b52-4ce8-8c6d-b3c1d84284e4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_311f4f6b-902d-45fe-83bc-eab524e99b19" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_5c4e576d-8b52-4ce8-8c6d-b3c1d84284e4" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_311f4f6b-902d-45fe-83bc-eab524e99b19" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CommercialAndIndustrialPortfolioSegmentMember_b7eef72a-07ef-48d7-9fa1-746136158b77" xlink:href="efsc-20221231.xsd#efsc_CommercialAndIndustrialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_311f4f6b-902d-45fe-83bc-eab524e99b19" xlink:to="loc_efsc_CommercialAndIndustrialPortfolioSegmentMember_b7eef72a-07ef-48d7-9fa1-746136158b77" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CommercialRealEstateInvestorOwnedFinancingReceivableMember_cd2bb0de-61c8-4ee6-a0d5-9c75b24803bb" xlink:href="efsc-20221231.xsd#efsc_CommercialRealEstateInvestorOwnedFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_311f4f6b-902d-45fe-83bc-eab524e99b19" xlink:to="loc_efsc_CommercialRealEstateInvestorOwnedFinancingReceivableMember_cd2bb0de-61c8-4ee6-a0d5-9c75b24803bb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CommercialRealEstateOwnerOccupiedFinancingReceivableMember_279c5b4e-aa93-4943-a968-63fd33209ee4" xlink:href="efsc-20221231.xsd#efsc_CommercialRealEstateOwnerOccupiedFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_311f4f6b-902d-45fe-83bc-eab524e99b19" xlink:to="loc_efsc_CommercialRealEstateOwnerOccupiedFinancingReceivableMember_279c5b4e-aa93-4943-a968-63fd33209ee4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ResidentialRealEstateFinancingReceivableMember_a023143a-00c3-4cd6-ad79-48edecf12cae" xlink:href="efsc-20221231.xsd#efsc_ResidentialRealEstateFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_311f4f6b-902d-45fe-83bc-eab524e99b19" xlink:to="loc_efsc_ResidentialRealEstateFinancingReceivableMember_a023143a-00c3-4cd6-ad79-48edecf12cae" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ConsumerAndOtherPortfolioSegmentMember_e9d2dbcf-8582-4abc-b9ab-9b53e7960eae" xlink:href="efsc-20221231.xsd#efsc_ConsumerAndOtherPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_311f4f6b-902d-45fe-83bc-eab524e99b19" xlink:to="loc_efsc_ConsumerAndOtherPortfolioSegmentMember_e9d2dbcf-8582-4abc-b9ab-9b53e7960eae" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CollateralAxis_ec5e2ea7-0535-4072-895f-95ca1e4cf2de" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CollateralAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableTroubledDebtRestructuringsTable_93cc3cf2-8bba-4b82-828e-61ed189ef0c4" xlink:to="loc_us-gaap_CollateralAxis_ec5e2ea7-0535-4072-895f-95ca1e4cf2de" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CollateralDomain_ec5e2ea7-0535-4072-895f-95ca1e4cf2de_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CollateralDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CollateralAxis_ec5e2ea7-0535-4072-895f-95ca1e4cf2de" xlink:to="loc_us-gaap_CollateralDomain_ec5e2ea7-0535-4072-895f-95ca1e4cf2de_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CollateralDomain_404ee506-1985-416b-9b13-04999e218f61" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CollateralDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CollateralAxis_ec5e2ea7-0535-4072-895f-95ca1e4cf2de" xlink:to="loc_us-gaap_CollateralDomain_404ee506-1985-416b-9b13-04999e218f61" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialRealEstateMember_733c4b93-ec53-42f4-b296-b58c3ab39cc7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommercialRealEstateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollateralDomain_404ee506-1985-416b-9b13-04999e218f61" xlink:to="loc_us-gaap_CommercialRealEstateMember_733c4b93-ec53-42f4-b296-b58c3ab39cc7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResidentialRealEstateMember_992b1480-2036-4136-b39d-a611d1cf0390" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ResidentialRealEstateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollateralDomain_404ee506-1985-416b-9b13-04999e218f61" xlink:to="loc_us-gaap_ResidentialRealEstateMember_992b1480-2036-4136-b39d-a611d1cf0390" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_BlanketLienMember_4c941c13-b7ad-42b8-89de-cc091b18ba95" xlink:href="efsc-20221231.xsd#efsc_BlanketLienMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CollateralDomain_404ee506-1985-416b-9b13-04999e218f61" xlink:to="loc_efsc_BlanketLienMember_4c941c13-b7ad-42b8-89de-cc091b18ba95" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_4d657754-319c-491e-8711-7b0f738b8cb1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivablePortfolioSegmentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableTroubledDebtRestructuringsTable_93cc3cf2-8bba-4b82-828e-61ed189ef0c4" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_4d657754-319c-491e-8711-7b0f738b8cb1" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_4d657754-319c-491e-8711-7b0f738b8cb1_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_4d657754-319c-491e-8711-7b0f738b8cb1" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_4d657754-319c-491e-8711-7b0f738b8cb1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_2568707f-2265-4ee4-9f96-2d9eb4f6b8a6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_4d657754-319c-491e-8711-7b0f738b8cb1" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_2568707f-2265-4ee4-9f96-2d9eb4f6b8a6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CommercialAndIndustrialPortfolioSegmentMember_34469fc6-3c4b-4e98-a1ee-bc8641eea4e3" xlink:href="efsc-20221231.xsd#efsc_CommercialAndIndustrialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_2568707f-2265-4ee4-9f96-2d9eb4f6b8a6" xlink:to="loc_efsc_CommercialAndIndustrialPortfolioSegmentMember_34469fc6-3c4b-4e98-a1ee-bc8641eea4e3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialPortfolioSegmentMember_333b7775-0c4b-4336-b1c2-4de34cf23f42" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommercialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_2568707f-2265-4ee4-9f96-2d9eb4f6b8a6" xlink:to="loc_us-gaap_CommercialPortfolioSegmentMember_333b7775-0c4b-4336-b1c2-4de34cf23f42" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResidentialPortfolioSegmentMember_e2a1a5ae-74e6-43ca-9a10-009769f22e00" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ResidentialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_2568707f-2265-4ee4-9f96-2d9eb4f6b8a6" xlink:to="loc_us-gaap_ResidentialPortfolioSegmentMember_e2a1a5ae-74e6-43ca-9a10-009769f22e00" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnallocatedFinancingReceivablesMember_ffb60f50-90c0-4709-84b0-ecec8aaf1fff" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_UnallocatedFinancingReceivablesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_2568707f-2265-4ee4-9f96-2d9eb4f6b8a6" xlink:to="loc_us-gaap_UnallocatedFinancingReceivablesMember_ffb60f50-90c0-4709-84b0-ecec8aaf1fff" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/PortfolioLoansSummaryofRecordedInvestmentbyCategoryforPortfolioLoansRestructuredandSubsequentlyDefaultedDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#PortfolioLoansSummaryofRecordedInvestmentbyCategoryforPortfolioLoansRestructuredandSubsequentlyDefaultedDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/PortfolioLoansSummaryofRecordedInvestmentbyCategoryforPortfolioLoansRestructuredandSubsequentlyDefaultedDetails" xlink:type="extended" id="ibac0b7034a5b4ef89856081fd9238e04_PortfolioLoansSummaryofRecordedInvestmentbyCategoryforPortfolioLoansRestructuredandSubsequentlyDefaultedDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableModificationsLineItems_64f5b4f3-0b6a-4691-9872-d5d8fece261c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableModificationsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableModificationsSubsequentDefaultNumberOfContracts1_f16edd5b-1337-41cf-890c-fd3028dfc9de" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableModificationsSubsequentDefaultNumberOfContracts1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableModificationsLineItems_64f5b4f3-0b6a-4691-9872-d5d8fece261c" xlink:to="loc_us-gaap_FinancingReceivableModificationsSubsequentDefaultNumberOfContracts1_f16edd5b-1337-41cf-890c-fd3028dfc9de" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableModificationsSubsequentDefaultRecordedInvestment1_8016619a-bdee-4fdb-8b35-ed1a8db33935" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableModificationsSubsequentDefaultRecordedInvestment1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableModificationsLineItems_64f5b4f3-0b6a-4691-9872-d5d8fece261c" xlink:to="loc_us-gaap_FinancingReceivableModificationsSubsequentDefaultRecordedInvestment1_8016619a-bdee-4fdb-8b35-ed1a8db33935" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFinancingReceivableTroubledDebtRestructuringsTable_e829ddc9-1fff-477d-87c7-e9022eab64de" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfFinancingReceivableTroubledDebtRestructuringsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FinancingReceivableModificationsLineItems_64f5b4f3-0b6a-4691-9872-d5d8fece261c" xlink:to="loc_us-gaap_ScheduleOfFinancingReceivableTroubledDebtRestructuringsTable_e829ddc9-1fff-477d-87c7-e9022eab64de" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansByMajorCategoryAxis_c3742d14-9d97-48aa-ad33-e074e3a9f2e0" xlink:href="efsc-20221231.xsd#efsc_LoansByMajorCategoryAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableTroubledDebtRestructuringsTable_e829ddc9-1fff-477d-87c7-e9022eab64de" xlink:to="loc_efsc_LoansByMajorCategoryAxis_c3742d14-9d97-48aa-ad33-e074e3a9f2e0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansByMajorCategoryDomain_c3742d14-9d97-48aa-ad33-e074e3a9f2e0_default" xlink:href="efsc-20221231.xsd#efsc_LoansByMajorCategoryDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_efsc_LoansByMajorCategoryAxis_c3742d14-9d97-48aa-ad33-e074e3a9f2e0" xlink:to="loc_efsc_LoansByMajorCategoryDomain_c3742d14-9d97-48aa-ad33-e074e3a9f2e0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansByMajorCategoryDomain_bee332ea-3731-438d-a922-0142f4af9bd1" xlink:href="efsc-20221231.xsd#efsc_LoansByMajorCategoryDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_efsc_LoansByMajorCategoryAxis_c3742d14-9d97-48aa-ad33-e074e3a9f2e0" xlink:to="loc_efsc_LoansByMajorCategoryDomain_bee332ea-3731-438d-a922-0142f4af9bd1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_NoncoveredLoansMember_4daf9338-4cee-4ea1-8fa6-67b49aeab8f8" xlink:href="efsc-20221231.xsd#efsc_NoncoveredLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_efsc_LoansByMajorCategoryDomain_bee332ea-3731-438d-a922-0142f4af9bd1" xlink:to="loc_efsc_NoncoveredLoansMember_4daf9338-4cee-4ea1-8fa6-67b49aeab8f8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_dce5d634-2154-46d5-9feb-d3539a4284f9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableTroubledDebtRestructuringsTable_e829ddc9-1fff-477d-87c7-e9022eab64de" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_dce5d634-2154-46d5-9feb-d3539a4284f9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_dce5d634-2154-46d5-9feb-d3539a4284f9_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_dce5d634-2154-46d5-9feb-d3539a4284f9" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_dce5d634-2154-46d5-9feb-d3539a4284f9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_36b4121c-84c6-4755-a701-f05458f49008" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_dce5d634-2154-46d5-9feb-d3539a4284f9" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_36b4121c-84c6-4755-a701-f05458f49008" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResidentialRealEstateMember_417fd3dc-3b4c-448e-8034-1a8f08f572f1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ResidentialRealEstateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_36b4121c-84c6-4755-a701-f05458f49008" xlink:to="loc_us-gaap_ResidentialRealEstateMember_417fd3dc-3b4c-448e-8034-1a8f08f572f1" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/PortfolioLoansSummaryofAgingofRecordedInvestmentinPastDuePortfolioLoansbyPortfolioClassandCategoryDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#PortfolioLoansSummaryofAgingofRecordedInvestmentinPastDuePortfolioLoansbyPortfolioClassandCategoryDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/PortfolioLoansSummaryofAgingofRecordedInvestmentinPastDuePortfolioLoansbyPortfolioClassandCategoryDetails" xlink:type="extended" id="i206ed496630a433c9c66dd6388d9561c_PortfolioLoansSummaryofAgingofRecordedInvestmentinPastDuePortfolioLoansbyPortfolioClassandCategoryDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentPastDueLineItems_d5e94d18-ace6-476c-95bc-840c586371a7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentPastDueLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NotesReceivableGross_b9ba3004-6c46-4214-8a16-bf4c7d6d2354" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NotesReceivableGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentPastDueLineItems_d5e94d18-ace6-476c-95bc-840c586371a7" xlink:to="loc_us-gaap_NotesReceivableGross_b9ba3004-6c46-4214-8a16-bf4c7d6d2354" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableCoveredAfterAllowanceForCreditLoss_3798217b-b4c3-4c52-b9e6-c621a5db7f84" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableCoveredAfterAllowanceForCreditLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentPastDueLineItems_d5e94d18-ace6-476c-95bc-840c586371a7" xlink:to="loc_us-gaap_FinancingReceivableCoveredAfterAllowanceForCreditLoss_3798217b-b4c3-4c52-b9e6-c621a5db7f84" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableBeforeAllowanceForCreditLossAndFee_ebdc94e5-d736-4c8c-ac86-2ec48ad95fa3" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableBeforeAllowanceForCreditLossAndFee"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentPastDueLineItems_d5e94d18-ace6-476c-95bc-840c586371a7" xlink:to="loc_us-gaap_FinancingReceivableBeforeAllowanceForCreditLossAndFee_ebdc94e5-d736-4c8c-ac86-2ec48ad95fa3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_FinancingReceivableCoveredAfterAllowanceForCreditLossAndUnearnedLoanFeesNet_b7667f3a-e2cc-4141-a6d6-b86d7ea2686c" xlink:href="efsc-20221231.xsd#efsc_FinancingReceivableCoveredAfterAllowanceForCreditLossAndUnearnedLoanFeesNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentPastDueLineItems_d5e94d18-ace6-476c-95bc-840c586371a7" xlink:to="loc_efsc_FinancingReceivableCoveredAfterAllowanceForCreditLossAndUnearnedLoanFeesNet_b7667f3a-e2cc-4141-a6d6-b86d7ea2686c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_FinancingReceivableUnearnedLoanFeesNet_2a08aace-5a16-48e7-baf3-a30b822ca051" xlink:href="efsc-20221231.xsd#efsc_FinancingReceivableUnearnedLoanFeesNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentPastDueLineItems_d5e94d18-ace6-476c-95bc-840c586371a7" xlink:to="loc_efsc_FinancingReceivableUnearnedLoanFeesNet_2a08aace-5a16-48e7-baf3-a30b822ca051" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFinancingReceivablesPastDueTable_e07538d5-1209-4452-ad39-692c5597f830" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfFinancingReceivablesPastDueTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentPastDueLineItems_d5e94d18-ace6-476c-95bc-840c586371a7" xlink:to="loc_us-gaap_ScheduleOfFinancingReceivablesPastDueTable_e07538d5-1209-4452-ad39-692c5597f830" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansByMajorCategoryAxis_37d830b8-7df5-4376-afa8-fa3ef2b6f087" xlink:href="efsc-20221231.xsd#efsc_LoansByMajorCategoryAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivablesPastDueTable_e07538d5-1209-4452-ad39-692c5597f830" xlink:to="loc_efsc_LoansByMajorCategoryAxis_37d830b8-7df5-4376-afa8-fa3ef2b6f087" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansByMajorCategoryDomain_37d830b8-7df5-4376-afa8-fa3ef2b6f087_default" xlink:href="efsc-20221231.xsd#efsc_LoansByMajorCategoryDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_efsc_LoansByMajorCategoryAxis_37d830b8-7df5-4376-afa8-fa3ef2b6f087" xlink:to="loc_efsc_LoansByMajorCategoryDomain_37d830b8-7df5-4376-afa8-fa3ef2b6f087_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansByMajorCategoryDomain_5326e4a1-2d7f-4027-bb2a-eb4e3ead31c1" xlink:href="efsc-20221231.xsd#efsc_LoansByMajorCategoryDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_efsc_LoansByMajorCategoryAxis_37d830b8-7df5-4376-afa8-fa3ef2b6f087" xlink:to="loc_efsc_LoansByMajorCategoryDomain_5326e4a1-2d7f-4027-bb2a-eb4e3ead31c1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_NoncoveredLoansMember_bcf50727-4db1-4df5-8eba-6f9f16bf1953" xlink:href="efsc-20221231.xsd#efsc_NoncoveredLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_efsc_LoansByMajorCategoryDomain_5326e4a1-2d7f-4027-bb2a-eb4e3ead31c1" xlink:to="loc_efsc_NoncoveredLoansMember_bcf50727-4db1-4df5-8eba-6f9f16bf1953" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_852e7832-7071-4577-b739-e668a16d1347" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivablesPastDueTable_e07538d5-1209-4452-ad39-692c5597f830" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_852e7832-7071-4577-b739-e668a16d1347" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_852e7832-7071-4577-b739-e668a16d1347_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_852e7832-7071-4577-b739-e668a16d1347" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_852e7832-7071-4577-b739-e668a16d1347_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_ec862629-378e-4fa2-8440-360c1af87594" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_852e7832-7071-4577-b739-e668a16d1347" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_ec862629-378e-4fa2-8440-360c1af87594" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CommercialAndIndustrialPortfolioSegmentMember_bb704711-7719-4044-b8ce-82d98ee0f839" xlink:href="efsc-20221231.xsd#efsc_CommercialAndIndustrialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_ec862629-378e-4fa2-8440-360c1af87594" xlink:to="loc_efsc_CommercialAndIndustrialPortfolioSegmentMember_bb704711-7719-4044-b8ce-82d98ee0f839" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CommercialRealEstateInvestorOwnedFinancingReceivableMember_92642637-4a7e-4be1-924e-12ecb8dc14aa" xlink:href="efsc-20221231.xsd#efsc_CommercialRealEstateInvestorOwnedFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_ec862629-378e-4fa2-8440-360c1af87594" xlink:to="loc_efsc_CommercialRealEstateInvestorOwnedFinancingReceivableMember_92642637-4a7e-4be1-924e-12ecb8dc14aa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CommercialRealEstateOwnerOccupiedFinancingReceivableMember_510f8d6b-865d-4207-a8cf-95a9afcda711" xlink:href="efsc-20221231.xsd#efsc_CommercialRealEstateOwnerOccupiedFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_ec862629-378e-4fa2-8440-360c1af87594" xlink:to="loc_efsc_CommercialRealEstateOwnerOccupiedFinancingReceivableMember_510f8d6b-865d-4207-a8cf-95a9afcda711" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ConstructionAndLandDevelopmentFinancingReceivableMember_6cd2daba-2ae0-4a9a-95c7-8dabde869d9a" xlink:href="efsc-20221231.xsd#efsc_ConstructionAndLandDevelopmentFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_ec862629-378e-4fa2-8440-360c1af87594" xlink:to="loc_efsc_ConstructionAndLandDevelopmentFinancingReceivableMember_6cd2daba-2ae0-4a9a-95c7-8dabde869d9a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ResidentialRealEstateFinancingReceivableMember_b36f9746-679d-421f-a6d1-d71c4ae75eae" xlink:href="efsc-20221231.xsd#efsc_ResidentialRealEstateFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_ec862629-378e-4fa2-8440-360c1af87594" xlink:to="loc_efsc_ResidentialRealEstateFinancingReceivableMember_b36f9746-679d-421f-a6d1-d71c4ae75eae" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ConsumerAndOtherPortfolioSegmentMember_9ec3bba2-7985-41b6-aae4-48eeade28b59" xlink:href="efsc-20221231.xsd#efsc_ConsumerAndOtherPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_ec862629-378e-4fa2-8440-360c1af87594" xlink:to="loc_efsc_ConsumerAndOtherPortfolioSegmentMember_9ec3bba2-7985-41b6-aae4-48eeade28b59" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablesPeriodPastDueAxis_21a3622d-fc5d-4f24-bf00-624f2aca6d70" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivablesPeriodPastDueAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivablesPastDueTable_e07538d5-1209-4452-ad39-692c5597f830" xlink:to="loc_us-gaap_FinancingReceivablesPeriodPastDueAxis_21a3622d-fc5d-4f24-bf00-624f2aca6d70" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablesPeriodPastDueDomain_21a3622d-fc5d-4f24-bf00-624f2aca6d70_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivablesPeriodPastDueDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivablesPeriodPastDueAxis_21a3622d-fc5d-4f24-bf00-624f2aca6d70" xlink:to="loc_us-gaap_FinancingReceivablesPeriodPastDueDomain_21a3622d-fc5d-4f24-bf00-624f2aca6d70_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablesPeriodPastDueDomain_31c9a11e-f940-4e3f-8540-98617f4fad4b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivablesPeriodPastDueDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivablesPeriodPastDueAxis_21a3622d-fc5d-4f24-bf00-624f2aca6d70" xlink:to="loc_us-gaap_FinancingReceivablesPeriodPastDueDomain_31c9a11e-f940-4e3f-8540-98617f4fad4b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_FinancialAsset3089DaysPastDueMember_6b7b933e-0990-4fb9-b101-58efc2e72cbc" xlink:href="efsc-20221231.xsd#efsc_FinancialAsset3089DaysPastDueMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablesPeriodPastDueDomain_31c9a11e-f940-4e3f-8540-98617f4fad4b" xlink:to="loc_efsc_FinancialAsset3089DaysPastDueMember_6b7b933e-0990-4fb9-b101-58efc2e72cbc" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablesEqualToGreaterThan90DaysPastDueMember_270ae8a7-0eee-4d27-871a-2edcd428fdea" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivablesEqualToGreaterThan90DaysPastDueMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablesPeriodPastDueDomain_31c9a11e-f940-4e3f-8540-98617f4fad4b" xlink:to="loc_us-gaap_FinancingReceivablesEqualToGreaterThan90DaysPastDueMember_270ae8a7-0eee-4d27-871a-2edcd428fdea" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialAssetPastDueMember_743a2020-2a13-4f92-954e-9792283e7a75" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancialAssetPastDueMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablesPeriodPastDueDomain_31c9a11e-f940-4e3f-8540-98617f4fad4b" xlink:to="loc_us-gaap_FinancialAssetPastDueMember_743a2020-2a13-4f92-954e-9792283e7a75" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialAssetNotPastDueMember_aa2c3248-b9e4-4f11-97cc-4b66283cc601" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancialAssetNotPastDueMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablesPeriodPastDueDomain_31c9a11e-f940-4e3f-8540-98617f4fad4b" xlink:to="loc_us-gaap_FinancialAssetNotPastDueMember_aa2c3248-b9e4-4f11-97cc-4b66283cc601" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/PortfolioLoansSummaryofTermLoansbyOriginationYearDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#PortfolioLoansSummaryofTermLoansbyOriginationYearDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/PortfolioLoansSummaryofTermLoansbyOriginationYearDetails" xlink:type="extended" id="i630a3a60caf94b2f92ccacab89fb3b8e_PortfolioLoansSummaryofTermLoansbyOriginationYearDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_ad688839-6bdb-4e08-ba22-60cc37b60d28" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableOriginatedInCurrentFiscalYear_59315dfc-6e67-4996-bd7b-2273eaa5a5a5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableOriginatedInCurrentFiscalYear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_ad688839-6bdb-4e08-ba22-60cc37b60d28" xlink:to="loc_us-gaap_FinancingReceivableOriginatedInCurrentFiscalYear_59315dfc-6e67-4996-bd7b-2273eaa5a5a5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableOriginatedInFiscalYearBeforeLatestFiscalYear_1ce66fea-f581-4204-84c2-0f78c39a3551" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableOriginatedInFiscalYearBeforeLatestFiscalYear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_ad688839-6bdb-4e08-ba22-60cc37b60d28" xlink:to="loc_us-gaap_FinancingReceivableOriginatedInFiscalYearBeforeLatestFiscalYear_1ce66fea-f581-4204-84c2-0f78c39a3551" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableOriginatedTwoYearsBeforeLatestFiscalYear_13f20d77-838e-4d71-8b54-098740052adc" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableOriginatedTwoYearsBeforeLatestFiscalYear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_ad688839-6bdb-4e08-ba22-60cc37b60d28" xlink:to="loc_us-gaap_FinancingReceivableOriginatedTwoYearsBeforeLatestFiscalYear_13f20d77-838e-4d71-8b54-098740052adc" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableOriginatedThreeYearsBeforeLatestFiscalYear_787313e1-6736-41ad-b708-d62e72fd737b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableOriginatedThreeYearsBeforeLatestFiscalYear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_ad688839-6bdb-4e08-ba22-60cc37b60d28" xlink:to="loc_us-gaap_FinancingReceivableOriginatedThreeYearsBeforeLatestFiscalYear_787313e1-6736-41ad-b708-d62e72fd737b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableOriginatedFourYearsBeforeLatestFiscalYear_c3371b76-06a0-48de-9a72-5319eee99c93" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableOriginatedFourYearsBeforeLatestFiscalYear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_ad688839-6bdb-4e08-ba22-60cc37b60d28" xlink:to="loc_us-gaap_FinancingReceivableOriginatedFourYearsBeforeLatestFiscalYear_c3371b76-06a0-48de-9a72-5319eee99c93" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableOriginatedFiveOrMoreYearsBeforeLatestFiscalYear_6754f59c-d3ea-4a41-93bb-97ce05fb9b48" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableOriginatedFiveOrMoreYearsBeforeLatestFiscalYear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_ad688839-6bdb-4e08-ba22-60cc37b60d28" xlink:to="loc_us-gaap_FinancingReceivableOriginatedFiveOrMoreYearsBeforeLatestFiscalYear_6754f59c-d3ea-4a41-93bb-97ce05fb9b48" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_FinancingReceivableConverted_51a1a5f3-8854-419d-8238-d89abc77facd" xlink:href="efsc-20221231.xsd#efsc_FinancingReceivableConverted"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_ad688839-6bdb-4e08-ba22-60cc37b60d28" xlink:to="loc_efsc_FinancingReceivableConverted_51a1a5f3-8854-419d-8238-d89abc77facd" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRevolving_76ee6553-2534-46a6-b9e6-2e95aab2d106" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRevolving"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_ad688839-6bdb-4e08-ba22-60cc37b60d28" xlink:to="loc_us-gaap_FinancingReceivableRevolving_76ee6553-2534-46a6-b9e6-2e95aab2d106" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NotesReceivableGross_d5447df9-4da9-4aa8-b26e-69693579d0cf" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NotesReceivableGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_ad688839-6bdb-4e08-ba22-60cc37b60d28" xlink:to="loc_us-gaap_NotesReceivableGross_d5447df9-4da9-4aa8-b26e-69693579d0cf" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_e20f50d3-5639-4b4f-a73b-a9d8e9e66b48" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_ad688839-6bdb-4e08-ba22-60cc37b60d28" xlink:to="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_e20f50d3-5639-4b4f-a73b-a9d8e9e66b48" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_0a30a279-0e84-48c5-a6e2-61456bb2f2bb" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivablePortfolioSegmentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_e20f50d3-5639-4b4f-a73b-a9d8e9e66b48" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_0a30a279-0e84-48c5-a6e2-61456bb2f2bb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_0a30a279-0e84-48c5-a6e2-61456bb2f2bb_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_0a30a279-0e84-48c5-a6e2-61456bb2f2bb" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_0a30a279-0e84-48c5-a6e2-61456bb2f2bb_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_a83f86ce-726f-4e51-8774-84ab0247441d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_0a30a279-0e84-48c5-a6e2-61456bb2f2bb" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_a83f86ce-726f-4e51-8774-84ab0247441d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CommercialAndIndustrialPortfolioSegmentMember_28106b13-5a1c-4bc4-a948-c9d207400d59" xlink:href="efsc-20221231.xsd#efsc_CommercialAndIndustrialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_a83f86ce-726f-4e51-8774-84ab0247441d" xlink:to="loc_efsc_CommercialAndIndustrialPortfolioSegmentMember_28106b13-5a1c-4bc4-a948-c9d207400d59" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CommercialRealEstateInvestorOwnedPortfolioSegmentMember_843c0329-efd0-4eb6-a47c-e2c1ea7e93f9" xlink:href="efsc-20221231.xsd#efsc_CommercialRealEstateInvestorOwnedPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_a83f86ce-726f-4e51-8774-84ab0247441d" xlink:to="loc_efsc_CommercialRealEstateInvestorOwnedPortfolioSegmentMember_843c0329-efd0-4eb6-a47c-e2c1ea7e93f9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CommercialRealEstateOwnerOccupiedPortfolioSegmentMember_6a284a16-a188-452c-853a-81020849f8b0" xlink:href="efsc-20221231.xsd#efsc_CommercialRealEstateOwnerOccupiedPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_a83f86ce-726f-4e51-8774-84ab0247441d" xlink:to="loc_efsc_CommercialRealEstateOwnerOccupiedPortfolioSegmentMember_6a284a16-a188-452c-853a-81020849f8b0" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ConstructionRealEstatePortfolioSegmentMember_7bc684e5-107f-4600-8bc2-2eaf058415b0" xlink:href="efsc-20221231.xsd#efsc_ConstructionRealEstatePortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_a83f86ce-726f-4e51-8774-84ab0247441d" xlink:to="loc_efsc_ConstructionRealEstatePortfolioSegmentMember_7bc684e5-107f-4600-8bc2-2eaf058415b0" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResidentialPortfolioSegmentMember_ad8086db-d8fc-40ac-9934-0a4878b1c381" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ResidentialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_a83f86ce-726f-4e51-8774-84ab0247441d" xlink:to="loc_us-gaap_ResidentialPortfolioSegmentMember_ad8086db-d8fc-40ac-9934-0a4878b1c381" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ConsumerAndOtherPortfolioSegmentMember_4ca904fa-33d1-4f6f-80dd-a9ca0fccf09f" xlink:href="efsc-20221231.xsd#efsc_ConsumerAndOtherPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_a83f86ce-726f-4e51-8774-84ab0247441d" xlink:to="loc_efsc_ConsumerAndOtherPortfolioSegmentMember_4ca904fa-33d1-4f6f-80dd-a9ca0fccf09f" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansClassifiedByRiskCategoryMember_afd28f49-cd84-4710-b674-c7028a94e7fa" xlink:href="efsc-20221231.xsd#efsc_LoansClassifiedByRiskCategoryMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_a83f86ce-726f-4e51-8774-84ab0247441d" xlink:to="loc_efsc_LoansClassifiedByRiskCategoryMember_afd28f49-cd84-4710-b674-c7028a94e7fa" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansClassifiedByPerformingStatusMember_baacfe7c-2e7f-4db7-b6b1-f6a3efd400ba" xlink:href="efsc-20221231.xsd#efsc_LoansClassifiedByPerformingStatusMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_a83f86ce-726f-4e51-8774-84ab0247441d" xlink:to="loc_efsc_LoansClassifiedByPerformingStatusMember_baacfe7c-2e7f-4db7-b6b1-f6a3efd400ba" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InternalCreditAssessmentAxis_24f63a04-c117-4a29-86df-0389964b8bb8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InternalCreditAssessmentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_e20f50d3-5639-4b4f-a73b-a9d8e9e66b48" xlink:to="loc_us-gaap_InternalCreditAssessmentAxis_24f63a04-c117-4a29-86df-0389964b8bb8" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InternalCreditAssessmentDomain_24f63a04-c117-4a29-86df-0389964b8bb8_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InternalCreditAssessmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_InternalCreditAssessmentAxis_24f63a04-c117-4a29-86df-0389964b8bb8" xlink:to="loc_us-gaap_InternalCreditAssessmentDomain_24f63a04-c117-4a29-86df-0389964b8bb8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InternalCreditAssessmentDomain_8cf9fafe-4cc9-4502-8f7d-63c2bd3897e9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InternalCreditAssessmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_InternalCreditAssessmentAxis_24f63a04-c117-4a29-86df-0389964b8bb8" xlink:to="loc_us-gaap_InternalCreditAssessmentDomain_8cf9fafe-4cc9-4502-8f7d-63c2bd3897e9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PassMember_92222f43-1d44-4e08-b9f4-e62021a23bfa" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PassMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InternalCreditAssessmentDomain_8cf9fafe-4cc9-4502-8f7d-63c2bd3897e9" xlink:to="loc_us-gaap_PassMember_92222f43-1d44-4e08-b9f4-e62021a23bfa" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SpecialMentionMember_35cabf93-c08c-40c3-b09c-a3283abfdd33" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SpecialMentionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InternalCreditAssessmentDomain_8cf9fafe-4cc9-4502-8f7d-63c2bd3897e9" xlink:to="loc_us-gaap_SpecialMentionMember_35cabf93-c08c-40c3-b09c-a3283abfdd33" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubstandardMember_2e6f74db-10fb-4558-b3e9-206d174e9c39" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SubstandardMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InternalCreditAssessmentDomain_8cf9fafe-4cc9-4502-8f7d-63c2bd3897e9" xlink:to="loc_us-gaap_SubstandardMember_2e6f74db-10fb-4558-b3e9-206d174e9c39" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/PortfolioLoansCreditQualityBasedonAgingStatusDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#PortfolioLoansCreditQualityBasedonAgingStatusDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/PortfolioLoansCreditQualityBasedonAgingStatusDetails" xlink:type="extended" id="ia669ced1e4b34b058b494e0b7b8184b4_PortfolioLoansCreditQualityBasedonAgingStatusDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_e8c1f076-6a23-4243-8f5a-8c30068bf19d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NotesReceivableGross_bd087588-3b89-4110-be6c-9ecfef3aff99" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NotesReceivableGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_e8c1f076-6a23-4243-8f5a-8c30068bf19d" xlink:to="loc_us-gaap_NotesReceivableGross_bd087588-3b89-4110-be6c-9ecfef3aff99" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NotesReceivableNet_ab007cae-48e2-497d-b794-f04acf098f26" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NotesReceivableNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_e8c1f076-6a23-4243-8f5a-8c30068bf19d" xlink:to="loc_us-gaap_NotesReceivableNet_ab007cae-48e2-497d-b794-f04acf098f26" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_e8e3d113-4917-4291-a350-180e50492c0a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_e8c1f076-6a23-4243-8f5a-8c30068bf19d" xlink:to="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_e8e3d113-4917-4291-a350-180e50492c0a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentPerformanceStatusAxis_9b59ff0c-820c-46c6-be7f-357b6b993583" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancialInstrumentPerformanceStatusAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_e8e3d113-4917-4291-a350-180e50492c0a" xlink:to="loc_us-gaap_FinancialInstrumentPerformanceStatusAxis_9b59ff0c-820c-46c6-be7f-357b6b993583" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentPerformanceStatusDomain_9b59ff0c-820c-46c6-be7f-357b6b993583_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancialInstrumentPerformanceStatusDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentPerformanceStatusAxis_9b59ff0c-820c-46c6-be7f-357b6b993583" xlink:to="loc_us-gaap_FinancialInstrumentPerformanceStatusDomain_9b59ff0c-820c-46c6-be7f-357b6b993583_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentPerformanceStatusDomain_641848df-9fdf-4e97-bbc4-f81615e3f775" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancialInstrumentPerformanceStatusDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentPerformanceStatusAxis_9b59ff0c-820c-46c6-be7f-357b6b993583" xlink:to="loc_us-gaap_FinancialInstrumentPerformanceStatusDomain_641848df-9fdf-4e97-bbc4-f81615e3f775" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PerformingFinancingReceivableMember_904dcedb-e8b2-4431-8f8a-af6c2ea8624c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PerformingFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentPerformanceStatusDomain_641848df-9fdf-4e97-bbc4-f81615e3f775" xlink:to="loc_us-gaap_PerformingFinancingReceivableMember_904dcedb-e8b2-4431-8f8a-af6c2ea8624c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonperformingFinancingReceivableMember_73e3b313-fe8d-4f50-80eb-fa5f5e6818c1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NonperformingFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentPerformanceStatusDomain_641848df-9fdf-4e97-bbc4-f81615e3f775" xlink:to="loc_us-gaap_NonperformingFinancingReceivableMember_73e3b313-fe8d-4f50-80eb-fa5f5e6818c1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_80401f4f-c15e-4a43-b297-658124bb62e3" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivablePortfolioSegmentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_e8e3d113-4917-4291-a350-180e50492c0a" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_80401f4f-c15e-4a43-b297-658124bb62e3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_80401f4f-c15e-4a43-b297-658124bb62e3_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_80401f4f-c15e-4a43-b297-658124bb62e3" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_80401f4f-c15e-4a43-b297-658124bb62e3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_8d9e557a-b217-4660-af0a-ac076de694df" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_80401f4f-c15e-4a43-b297-658124bb62e3" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_8d9e557a-b217-4660-af0a-ac076de694df" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CommercialAndIndustrialPortfolioSegmentMember_2975edc5-3673-4ca8-ab29-c76c70f8565d" xlink:href="efsc-20221231.xsd#efsc_CommercialAndIndustrialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_8d9e557a-b217-4660-af0a-ac076de694df" xlink:to="loc_efsc_CommercialAndIndustrialPortfolioSegmentMember_2975edc5-3673-4ca8-ab29-c76c70f8565d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResidentialPortfolioSegmentMember_12db5e58-529f-4d75-ba1b-0c2cc2cfbcc1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ResidentialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_8d9e557a-b217-4660-af0a-ac076de694df" xlink:to="loc_us-gaap_ResidentialPortfolioSegmentMember_12db5e58-529f-4d75-ba1b-0c2cc2cfbcc1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ConsumerAndOtherPortfolioSegmentMember_3f599740-bf8f-433f-9cf7-c7dd56e10bf9" xlink:href="efsc-20221231.xsd#efsc_ConsumerAndOtherPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_8d9e557a-b217-4660-af0a-ac076de694df" xlink:to="loc_efsc_ConsumerAndOtherPortfolioSegmentMember_3f599740-bf8f-433f-9cf7-c7dd56e10bf9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansByMajorCategoryAxis_91a1e017-550a-4580-8b30-ae8fe9583de5" xlink:href="efsc-20221231.xsd#efsc_LoansByMajorCategoryAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_e8e3d113-4917-4291-a350-180e50492c0a" xlink:to="loc_efsc_LoansByMajorCategoryAxis_91a1e017-550a-4580-8b30-ae8fe9583de5" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansByMajorCategoryDomain_91a1e017-550a-4580-8b30-ae8fe9583de5_default" xlink:href="efsc-20221231.xsd#efsc_LoansByMajorCategoryDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_efsc_LoansByMajorCategoryAxis_91a1e017-550a-4580-8b30-ae8fe9583de5" xlink:to="loc_efsc_LoansByMajorCategoryDomain_91a1e017-550a-4580-8b30-ae8fe9583de5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansByMajorCategoryDomain_0fbbcbbe-f721-4216-bdfe-a510141c5754" xlink:href="efsc-20221231.xsd#efsc_LoansByMajorCategoryDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_efsc_LoansByMajorCategoryAxis_91a1e017-550a-4580-8b30-ae8fe9583de5" xlink:to="loc_efsc_LoansByMajorCategoryDomain_0fbbcbbe-f721-4216-bdfe-a510141c5754" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_NoncoveredLoansMember_c5530be5-a194-4d97-b933-83a8fde55eeb" xlink:href="efsc-20221231.xsd#efsc_NoncoveredLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_efsc_LoansByMajorCategoryDomain_0fbbcbbe-f721-4216-bdfe-a510141c5754" xlink:to="loc_efsc_NoncoveredLoansMember_c5530be5-a194-4d97-b933-83a8fde55eeb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_49269368-537e-499e-9b65-521041f6cd53" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_e8e3d113-4917-4291-a350-180e50492c0a" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_49269368-537e-499e-9b65-521041f6cd53" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_49269368-537e-499e-9b65-521041f6cd53_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_49269368-537e-499e-9b65-521041f6cd53" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_49269368-537e-499e-9b65-521041f6cd53_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_43815ac1-2e9b-453c-b27e-aebea48c8117" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_49269368-537e-499e-9b65-521041f6cd53" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_43815ac1-2e9b-453c-b27e-aebea48c8117" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CommercialRealEstateInvestorOwnedFinancingReceivableMember_bb96224d-acfc-44a9-80f8-227376d266cd" xlink:href="efsc-20221231.xsd#efsc_CommercialRealEstateInvestorOwnedFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_43815ac1-2e9b-453c-b27e-aebea48c8117" xlink:to="loc_efsc_CommercialRealEstateInvestorOwnedFinancingReceivableMember_bb96224d-acfc-44a9-80f8-227376d266cd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CommercialRealEstateOwnerOccupiedFinancingReceivableMember_54772f69-8a87-48d4-ae89-8588c8aec194" xlink:href="efsc-20221231.xsd#efsc_CommercialRealEstateOwnerOccupiedFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_43815ac1-2e9b-453c-b27e-aebea48c8117" xlink:to="loc_efsc_CommercialRealEstateOwnerOccupiedFinancingReceivableMember_54772f69-8a87-48d4-ae89-8588c8aec194" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ConstructionAndLandDevelopmentFinancingReceivableMember_13be4085-c32a-4343-ba88-b9685e93947b" xlink:href="efsc-20221231.xsd#efsc_ConstructionAndLandDevelopmentFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_43815ac1-2e9b-453c-b27e-aebea48c8117" xlink:to="loc_efsc_ConstructionAndLandDevelopmentFinancingReceivableMember_13be4085-c32a-4343-ba88-b9685e93947b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InternalCreditAssessmentAxis_2e10461c-b417-4b6d-8c18-e76255015167" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InternalCreditAssessmentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_e8e3d113-4917-4291-a350-180e50492c0a" xlink:to="loc_us-gaap_InternalCreditAssessmentAxis_2e10461c-b417-4b6d-8c18-e76255015167" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InternalCreditAssessmentDomain_2e10461c-b417-4b6d-8c18-e76255015167_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InternalCreditAssessmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_InternalCreditAssessmentAxis_2e10461c-b417-4b6d-8c18-e76255015167" xlink:to="loc_us-gaap_InternalCreditAssessmentDomain_2e10461c-b417-4b6d-8c18-e76255015167_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InternalCreditAssessmentDomain_6d9ac712-aeef-4dda-b5b6-fea54ba38539" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InternalCreditAssessmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_InternalCreditAssessmentAxis_2e10461c-b417-4b6d-8c18-e76255015167" xlink:to="loc_us-gaap_InternalCreditAssessmentDomain_6d9ac712-aeef-4dda-b5b6-fea54ba38539" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PassMember_5e9e730a-4c96-4e65-b373-4d2d3c0a2fea" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PassMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InternalCreditAssessmentDomain_6d9ac712-aeef-4dda-b5b6-fea54ba38539" xlink:to="loc_us-gaap_PassMember_5e9e730a-4c96-4e65-b373-4d2d3c0a2fea" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SpecialMentionMember_4955e0dc-b88d-43a8-802c-2d30eaef0203" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SpecialMentionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InternalCreditAssessmentDomain_6d9ac712-aeef-4dda-b5b6-fea54ba38539" xlink:to="loc_us-gaap_SpecialMentionMember_4955e0dc-b88d-43a8-802c-2d30eaef0203" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubstandardMember_ea1d3029-bec8-4b29-b9ca-4db24a6a8d64" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SubstandardMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InternalCreditAssessmentDomain_6d9ac712-aeef-4dda-b5b6-fea54ba38539" xlink:to="loc_us-gaap_SubstandardMember_ea1d3029-bec8-4b29-b9ca-4db24a6a8d64" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/LeasesDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#LeasesDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/LeasesDetails" xlink:type="extended" id="i0b54b5a8c51c4482aee75974d9831405_LeasesDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeLeaseDescriptionLineItems_8ecafcb5-1545-4d44-ada0-65f3175c55dd" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LesseeLeaseDescriptionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths_5a03312e-5635-4ad8-9939-33f59df234d7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_8ecafcb5-1545-4d44-ada0-65f3175c55dd" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths_5a03312e-5635-4ad8-9939-33f59df234d7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseRightOfUseAsset_6878f1db-7743-4647-bfc4-6ef7c12c11ba" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OperatingLeaseRightOfUseAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_8ecafcb5-1545-4d44-ada0-65f3175c55dd" xlink:to="loc_us-gaap_OperatingLeaseRightOfUseAsset_6878f1db-7743-4647-bfc4-6ef7c12c11ba" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseCost_c2fc6d64-a470-444c-8005-b517cf12979a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OperatingLeaseCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_8ecafcb5-1545-4d44-ada0-65f3175c55dd" xlink:to="loc_us-gaap_OperatingLeaseCost_c2fc6d64-a470-444c-8005-b517cf12979a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RightOfUseAssetObtainedInExchangeForOperatingLeaseLiability_9c6cd673-b872-4ceb-8567-7114e2e82157" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RightOfUseAssetObtainedInExchangeForOperatingLeaseLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_8ecafcb5-1545-4d44-ada0-65f3175c55dd" xlink:to="loc_us-gaap_RightOfUseAssetObtainedInExchangeForOperatingLeaseLiability_9c6cd673-b872-4ceb-8567-7114e2e82157" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseRenewalTerm_abb26560-eaf4-41b7-a5d0-abfb6b8ba9fe" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LesseeOperatingLeaseRenewalTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_8ecafcb5-1545-4d44-ada0-65f3175c55dd" xlink:to="loc_us-gaap_LesseeOperatingLeaseRenewalTerm_abb26560-eaf4-41b7-a5d0-abfb6b8ba9fe" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShortTermLeaseCost_7603b77c-7776-48c2-ab1b-ad0f3191c060" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShortTermLeaseCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_8ecafcb5-1545-4d44-ada0-65f3175c55dd" xlink:to="loc_us-gaap_ShortTermLeaseCost_7603b77c-7776-48c2-ab1b-ad0f3191c060" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LeaseCost_1c703f2c-bec9-4584-a607-cd0834e9b973" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LeaseCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_8ecafcb5-1545-4d44-ada0-65f3175c55dd" xlink:to="loc_us-gaap_LeaseCost_1c703f2c-bec9-4584-a607-cd0834e9b973" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiability_71ac62c7-fa9b-4639-acd7-fa6f01be8ea4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OperatingLeaseLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_8ecafcb5-1545-4d44-ada0-65f3175c55dd" xlink:to="loc_us-gaap_OperatingLeaseLiability_71ac62c7-fa9b-4639-acd7-fa6f01be8ea4" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseWeightedAverageRemainingLeaseTerm1_528d2079-b7e8-41d1-abd2-f7cbf476ee8c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OperatingLeaseWeightedAverageRemainingLeaseTerm1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_8ecafcb5-1545-4d44-ada0-65f3175c55dd" xlink:to="loc_us-gaap_OperatingLeaseWeightedAverageRemainingLeaseTerm1_528d2079-b7e8-41d1-abd2-f7cbf476ee8c" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseWeightedAverageDiscountRatePercent_71603829-b943-405d-99d4-3aa47f7276f9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OperatingLeaseWeightedAverageDiscountRatePercent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_8ecafcb5-1545-4d44-ada0-65f3175c55dd" xlink:to="loc_us-gaap_OperatingLeaseWeightedAverageDiscountRatePercent_71603829-b943-405d-99d4-3aa47f7276f9" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo_317ac5c3-52f1-4abc-84e3-a20c034c2431" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_8ecafcb5-1545-4d44-ada0-65f3175c55dd" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo_317ac5c3-52f1-4abc-84e3-a20c034c2431" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree_c559c946-3da8-49f4-bf95-b2b5d55def7c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_8ecafcb5-1545-4d44-ada0-65f3175c55dd" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree_c559c946-3da8-49f4-bf95-b2b5d55def7c" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour_60b0729c-ed5c-4967-af15-0d3231e2f79e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_8ecafcb5-1545-4d44-ada0-65f3175c55dd" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour_60b0729c-ed5c-4967-af15-0d3231e2f79e" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFive_8ee4db8e-dfea-40d1-a4d4-f07d145bd903" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFive"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_8ecafcb5-1545-4d44-ada0-65f3175c55dd" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFive_8ee4db8e-dfea-40d1-a4d4-f07d145bd903" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueAfterYearFive_3bd10aac-c178-4aec-afa8-7b335bf33ddb" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueAfterYearFive"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_8ecafcb5-1545-4d44-ada0-65f3175c55dd" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueAfterYearFive_3bd10aac-c178-4aec-afa8-7b335bf33ddb" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_094a38dd-540e-4bcc-8f47-86144e5708d4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_8ecafcb5-1545-4d44-ada0-65f3175c55dd" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_094a38dd-540e-4bcc-8f47-86144e5708d4" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount_04eba4c6-a8c8-4967-b2b7-5fbaf1a64abc" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_8ecafcb5-1545-4d44-ada0-65f3175c55dd" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount_04eba4c6-a8c8-4967-b2b7-5fbaf1a64abc" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLeaseIncomeLeasePayments_850e4ba6-278e-4ff2-8ccd-e6811e39d95f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OperatingLeaseLeaseIncomeLeasePayments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_8ecafcb5-1545-4d44-ada0-65f3175c55dd" xlink:to="loc_us-gaap_OperatingLeaseLeaseIncomeLeasePayments_850e4ba6-278e-4ff2-8ccd-e6811e39d95f" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeasePayments_a59193c6-3dc8-481c-8cb6-fea6719f7142" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OperatingLeasePayments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_8ecafcb5-1545-4d44-ada0-65f3175c55dd" xlink:to="loc_us-gaap_OperatingLeasePayments_a59193c6-3dc8-481c-8cb6-fea6719f7142" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseRightOfUseAssetStatementOfFinancialPositionExtensibleList_a4f2955e-5d29-4767-be39-41496603dd06" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OperatingLeaseRightOfUseAssetStatementOfFinancialPositionExtensibleList"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_8ecafcb5-1545-4d44-ada0-65f3175c55dd" xlink:to="loc_us-gaap_OperatingLeaseRightOfUseAssetStatementOfFinancialPositionExtensibleList_a4f2955e-5d29-4767-be39-41496603dd06" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityStatementOfFinancialPositionExtensibleList_92d1f9c8-d44c-4353-88e3-0e9ac5236ee1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OperatingLeaseLiabilityStatementOfFinancialPositionExtensibleList"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_8ecafcb5-1545-4d44-ada0-65f3175c55dd" xlink:to="loc_us-gaap_OperatingLeaseLiabilityStatementOfFinancialPositionExtensibleList_92d1f9c8-d44c-4353-88e3-0e9ac5236ee1" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseImpairmentLoss_5d9aeae2-b2a8-4491-8e89-fb0237b32243" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OperatingLeaseImpairmentLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_8ecafcb5-1545-4d44-ada0-65f3175c55dd" xlink:to="loc_us-gaap_OperatingLeaseImpairmentLoss_5d9aeae2-b2a8-4491-8e89-fb0237b32243" xlink:type="arc" order="21"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeLeaseDescriptionTable_660b7a84-6fa8-47e2-ac61-5c527aec3d9f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LesseeLeaseDescriptionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_8ecafcb5-1545-4d44-ada0-65f3175c55dd" xlink:to="loc_us-gaap_LesseeLeaseDescriptionTable_660b7a84-6fa8-47e2-ac61-5c527aec3d9f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_7b62fce1-b831-49de-9121-980ac1b3bd0f" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LesseeLeaseDescriptionTable_660b7a84-6fa8-47e2-ac61-5c527aec3d9f" xlink:to="loc_srt_RangeAxis_7b62fce1-b831-49de-9121-980ac1b3bd0f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_7b62fce1-b831-49de-9121-980ac1b3bd0f_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_7b62fce1-b831-49de-9121-980ac1b3bd0f" xlink:to="loc_srt_RangeMember_7b62fce1-b831-49de-9121-980ac1b3bd0f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_f2e6cc72-0b74-4d8b-b2f4-b4e671afcd07" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_7b62fce1-b831-49de-9121-980ac1b3bd0f" xlink:to="loc_srt_RangeMember_f2e6cc72-0b74-4d8b-b2f4-b4e671afcd07" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_3182d1b6-90e4-4b85-8761-e6b3eac43198" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_f2e6cc72-0b74-4d8b-b2f4-b4e671afcd07" xlink:to="loc_srt_MaximumMember_3182d1b6-90e4-4b85-8761-e6b3eac43198" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/DerivativeFinancialInstrumentsDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#DerivativeFinancialInstrumentsDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/DerivativeFinancialInstrumentsDetails" xlink:type="extended" id="i572112bf32cd4cdbad69e68291797b65_DerivativeFinancialInstrumentsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeLineItems_1076bcfa-05d7-49d3-ae5b-21bb2dd4f8ec" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeAssetNotionalAmount_ced9f4de-1685-4f03-9db0-ce41a85bdfa0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeAssetNotionalAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_1076bcfa-05d7-49d3-ae5b-21bb2dd4f8ec" xlink:to="loc_us-gaap_DerivativeAssetNotionalAmount_ced9f4de-1685-4f03-9db0-ce41a85bdfa0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeFixedInterestRate_96a4f4f0-ed40-4af5-ad4a-805baeb68f4b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeFixedInterestRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_1076bcfa-05d7-49d3-ae5b-21bb2dd4f8ec" xlink:to="loc_us-gaap_DerivativeFixedInterestRate_96a4f4f0-ed40-4af5-ad4a-805baeb68f4b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeVariableInterestRate_02076f2a-3436-4c6d-abf4-a3bfc7fab8a7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeVariableInterestRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_1076bcfa-05d7-49d3-ae5b-21bb2dd4f8ec" xlink:to="loc_us-gaap_DerivativeVariableInterestRate_02076f2a-3436-4c6d-abf4-a3bfc7fab8a7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeNetLiabilityPositionAggregateFairValue_5d6a8c9b-842e-4bba-8f7b-873ad6347c84" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeNetLiabilityPositionAggregateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_1076bcfa-05d7-49d3-ae5b-21bb2dd4f8ec" xlink:to="loc_us-gaap_DerivativeNetLiabilityPositionAggregateFairValue_5d6a8c9b-842e-4bba-8f7b-873ad6347c84" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeCollateralRightToReclaimCash_8c863366-f70b-4f28-ba56-ee6ee6846791" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeCollateralRightToReclaimCash"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_1076bcfa-05d7-49d3-ae5b-21bb2dd4f8ec" xlink:to="loc_us-gaap_DerivativeCollateralRightToReclaimCash_8c863366-f70b-4f28-ba56-ee6ee6846791" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SummaryOfDerivativeInstrumentsAbstract_5313374b-114a-4cb0-8170-b3266a72e0d6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SummaryOfDerivativeInstrumentsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_1076bcfa-05d7-49d3-ae5b-21bb2dd4f8ec" xlink:to="loc_us-gaap_SummaryOfDerivativeInstrumentsAbstract_5313374b-114a-4cb0-8170-b3266a72e0d6" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeFairValueOfDerivativeAsset_42973ffc-5aa8-4233-9533-cb2276f4350b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeFairValueOfDerivativeAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SummaryOfDerivativeInstrumentsAbstract_5313374b-114a-4cb0-8170-b3266a72e0d6" xlink:to="loc_us-gaap_DerivativeFairValueOfDerivativeAsset_42973ffc-5aa8-4233-9533-cb2276f4350b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeFairValueOfDerivativeLiability_e0a9d12d-e8b9-4b26-88c2-ac01c4f5f982" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeFairValueOfDerivativeLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SummaryOfDerivativeInstrumentsAbstract_5313374b-114a-4cb0-8170-b3266a72e0d6" xlink:to="loc_us-gaap_DerivativeFairValueOfDerivativeLiability_e0a9d12d-e8b9-4b26-88c2-ac01c4f5f982" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeInstrumentsGainLossReclassifiedFromAccumulatedOCIIntoIncomeEffectivePortionNet_fdf8ee86-2d16-4d1d-8af5-d591ff53c1ee" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeInstrumentsGainLossReclassifiedFromAccumulatedOCIIntoIncomeEffectivePortionNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_1076bcfa-05d7-49d3-ae5b-21bb2dd4f8ec" xlink:to="loc_us-gaap_DerivativeInstrumentsGainLossReclassifiedFromAccumulatedOCIIntoIncomeEffectivePortionNet_fdf8ee86-2d16-4d1d-8af5-d591ff53c1ee" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeAssetFairValueGrossLiability_495ee084-1f04-43b2-aed2-a6fc45a88d9a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeAssetFairValueGrossLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_1076bcfa-05d7-49d3-ae5b-21bb2dd4f8ec" xlink:to="loc_us-gaap_DerivativeAssetFairValueGrossLiability_495ee084-1f04-43b2-aed2-a6fc45a88d9a" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeAssets_bd730d40-ae4c-4ff8-a0fe-655edd8b996e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_1076bcfa-05d7-49d3-ae5b-21bb2dd4f8ec" xlink:to="loc_us-gaap_DerivativeAssets_bd730d40-ae4c-4ff8-a0fe-655edd8b996e" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeCollateralObligationToReturnSecurities_9cda6dd4-b067-42eb-b4b0-5277e6925391" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeCollateralObligationToReturnSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_1076bcfa-05d7-49d3-ae5b-21bb2dd4f8ec" xlink:to="loc_us-gaap_DerivativeCollateralObligationToReturnSecurities_9cda6dd4-b067-42eb-b4b0-5277e6925391" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeCollateralObligationToReturnCash_4e2fa785-cf68-41c5-93a4-026219e6b57d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeCollateralObligationToReturnCash"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_1076bcfa-05d7-49d3-ae5b-21bb2dd4f8ec" xlink:to="loc_us-gaap_DerivativeCollateralObligationToReturnCash_4e2fa785-cf68-41c5-93a4-026219e6b57d" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeFairValueOfDerivativeAssetAmountNotOffsetAgainstCollateral_98a0027e-8077-42b6-a022-344edd89342e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeFairValueOfDerivativeAssetAmountNotOffsetAgainstCollateral"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_1076bcfa-05d7-49d3-ae5b-21bb2dd4f8ec" xlink:to="loc_us-gaap_DerivativeFairValueOfDerivativeAssetAmountNotOffsetAgainstCollateral_98a0027e-8077-42b6-a022-344edd89342e" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeLiabilityFairValueGrossAsset_a24402d2-e9c4-44d8-811f-74dfc9d482ba" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeLiabilityFairValueGrossAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_1076bcfa-05d7-49d3-ae5b-21bb2dd4f8ec" xlink:to="loc_us-gaap_DerivativeLiabilityFairValueGrossAsset_a24402d2-e9c4-44d8-811f-74dfc9d482ba" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeLiabilities_cf2ad9fa-3ffd-43f0-bef3-2dc73012b6e7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_1076bcfa-05d7-49d3-ae5b-21bb2dd4f8ec" xlink:to="loc_us-gaap_DerivativeLiabilities_cf2ad9fa-3ffd-43f0-bef3-2dc73012b6e7" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeCollateralRightToReclaimSecurities_c2956df2-1e8b-490a-9d36-55f0351d5a5a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeCollateralRightToReclaimSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_1076bcfa-05d7-49d3-ae5b-21bb2dd4f8ec" xlink:to="loc_us-gaap_DerivativeCollateralRightToReclaimSecurities_c2956df2-1e8b-490a-9d36-55f0351d5a5a" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeFairValueOfDerivativeLiabilityAmountOffsetAgainstCollateral_ea5723e4-e6b7-462c-82fc-7345f59f3f9e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeFairValueOfDerivativeLiabilityAmountOffsetAgainstCollateral"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_1076bcfa-05d7-49d3-ae5b-21bb2dd4f8ec" xlink:to="loc_us-gaap_DerivativeFairValueOfDerivativeLiabilityAmountOffsetAgainstCollateral_ea5723e4-e6b7-462c-82fc-7345f59f3f9e" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuritiesSoldUnderAgreementsToRepurchaseGross_9fd16d49-7635-49dd-9931-3604289f282a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SecuritiesSoldUnderAgreementsToRepurchaseGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_1076bcfa-05d7-49d3-ae5b-21bb2dd4f8ec" xlink:to="loc_us-gaap_SecuritiesSoldUnderAgreementsToRepurchaseGross_9fd16d49-7635-49dd-9931-3604289f282a" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuritiesSoldUnderAgreementsToRepurchaseAsset_d011ac4c-d75e-442b-abcc-5b71a6efd06f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SecuritiesSoldUnderAgreementsToRepurchaseAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_1076bcfa-05d7-49d3-ae5b-21bb2dd4f8ec" xlink:to="loc_us-gaap_SecuritiesSoldUnderAgreementsToRepurchaseAsset_d011ac4c-d75e-442b-abcc-5b71a6efd06f" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuritiesSoldUnderAgreementsToRepurchase_a39c8290-9217-4b35-9bed-a72752aae56a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SecuritiesSoldUnderAgreementsToRepurchase"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_1076bcfa-05d7-49d3-ae5b-21bb2dd4f8ec" xlink:to="loc_us-gaap_SecuritiesSoldUnderAgreementsToRepurchase_a39c8290-9217-4b35-9bed-a72752aae56a" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuritiesSoldUnderAgreementsToRepurchaseCollateralRightToReclaimSecurities_89fd5206-ee8f-4b24-a9f3-9bfe7783add6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SecuritiesSoldUnderAgreementsToRepurchaseCollateralRightToReclaimSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_1076bcfa-05d7-49d3-ae5b-21bb2dd4f8ec" xlink:to="loc_us-gaap_SecuritiesSoldUnderAgreementsToRepurchaseCollateralRightToReclaimSecurities_89fd5206-ee8f-4b24-a9f3-9bfe7783add6" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuritiesSoldUnderAgreementsToRepurchaseCollateralRightToReclaimCash_10cadb1f-e3b5-45f1-bcfc-505aa0ebbeff" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SecuritiesSoldUnderAgreementsToRepurchaseCollateralRightToReclaimCash"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_1076bcfa-05d7-49d3-ae5b-21bb2dd4f8ec" xlink:to="loc_us-gaap_SecuritiesSoldUnderAgreementsToRepurchaseCollateralRightToReclaimCash_10cadb1f-e3b5-45f1-bcfc-505aa0ebbeff" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuritiesSoldUnderAgreementsToRepurchaseAmountOffsetAgainstCollateral_a7f7c141-d321-45ec-9a54-ad6af9a1a0df" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SecuritiesSoldUnderAgreementsToRepurchaseAmountOffsetAgainstCollateral"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_1076bcfa-05d7-49d3-ae5b-21bb2dd4f8ec" xlink:to="loc_us-gaap_SecuritiesSoldUnderAgreementsToRepurchaseAmountOffsetAgainstCollateral_a7f7c141-d321-45ec-9a54-ad6af9a1a0df" xlink:type="arc" order="21"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeTable_74da6d7b-d12e-4bc2-904b-6ef85d4d6816" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DerivativeLineItems_1076bcfa-05d7-49d3-ae5b-21bb2dd4f8ec" xlink:to="loc_us-gaap_DerivativeTable_74da6d7b-d12e-4bc2-904b-6ef85d4d6816" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeInstrumentsGainLossByHedgingRelationshipAxis_c6fb0c4e-2cc8-4d6e-9621-b92b3611fa19" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeInstrumentsGainLossByHedgingRelationshipAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeTable_74da6d7b-d12e-4bc2-904b-6ef85d4d6816" xlink:to="loc_us-gaap_DerivativeInstrumentsGainLossByHedgingRelationshipAxis_c6fb0c4e-2cc8-4d6e-9621-b92b3611fa19" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HedgingRelationshipDomain_c6fb0c4e-2cc8-4d6e-9621-b92b3611fa19_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_HedgingRelationshipDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DerivativeInstrumentsGainLossByHedgingRelationshipAxis_c6fb0c4e-2cc8-4d6e-9621-b92b3611fa19" xlink:to="loc_us-gaap_HedgingRelationshipDomain_c6fb0c4e-2cc8-4d6e-9621-b92b3611fa19_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HedgingRelationshipDomain_f75311eb-79e7-40ee-893a-857069c99e1d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_HedgingRelationshipDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DerivativeInstrumentsGainLossByHedgingRelationshipAxis_c6fb0c4e-2cc8-4d6e-9621-b92b3611fa19" xlink:to="loc_us-gaap_HedgingRelationshipDomain_f75311eb-79e7-40ee-893a-857069c99e1d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashFlowHedgingMember_fc0fad0b-bd0b-46d3-8a69-48655d46fd8e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CashFlowHedgingMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HedgingRelationshipDomain_f75311eb-79e7-40ee-893a-857069c99e1d" xlink:to="loc_us-gaap_CashFlowHedgingMember_fc0fad0b-bd0b-46d3-8a69-48655d46fd8e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_PrimeBasedInterestRateCollarMember_29e15b69-bb5b-4aa3-b4a1-e4ea75f08d0b" xlink:href="efsc-20221231.xsd#efsc_PrimeBasedInterestRateCollarMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HedgingRelationshipDomain_f75311eb-79e7-40ee-893a-857069c99e1d" xlink:to="loc_efsc_PrimeBasedInterestRateCollarMember_29e15b69-bb5b-4aa3-b4a1-e4ea75f08d0b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementScenarioAxis_fa122672-6ec3-4d5b-bcfc-6599663a51b9" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_StatementScenarioAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeTable_74da6d7b-d12e-4bc2-904b-6ef85d4d6816" xlink:to="loc_srt_StatementScenarioAxis_fa122672-6ec3-4d5b-bcfc-6599663a51b9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_fa122672-6ec3-4d5b-bcfc-6599663a51b9_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementScenarioAxis_fa122672-6ec3-4d5b-bcfc-6599663a51b9" xlink:to="loc_srt_ScenarioUnspecifiedDomain_fa122672-6ec3-4d5b-bcfc-6599663a51b9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_9dfd19cd-e3dc-40a1-9317-b3aa9f6104b3" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementScenarioAxis_fa122672-6ec3-4d5b-bcfc-6599663a51b9" xlink:to="loc_srt_ScenarioUnspecifiedDomain_9dfd19cd-e3dc-40a1-9317-b3aa9f6104b3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioForecastMember_46220fe7-f123-44b5-840e-bf123b45abc4" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ScenarioForecastMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ScenarioUnspecifiedDomain_9dfd19cd-e3dc-40a1-9317-b3aa9f6104b3" xlink:to="loc_srt_ScenarioForecastMember_46220fe7-f123-44b5-840e-bf123b45abc4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HedgingDesignationAxis_f0b3ce2b-c112-464b-9d77-a731cd6d0f67" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_HedgingDesignationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeTable_74da6d7b-d12e-4bc2-904b-6ef85d4d6816" xlink:to="loc_us-gaap_HedgingDesignationAxis_f0b3ce2b-c112-464b-9d77-a731cd6d0f67" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HedgingDesignationDomain_f0b3ce2b-c112-464b-9d77-a731cd6d0f67_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_HedgingDesignationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_HedgingDesignationAxis_f0b3ce2b-c112-464b-9d77-a731cd6d0f67" xlink:to="loc_us-gaap_HedgingDesignationDomain_f0b3ce2b-c112-464b-9d77-a731cd6d0f67_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HedgingDesignationDomain_5cb97730-da30-4d64-865c-ac0ea2f25609" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_HedgingDesignationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_HedgingDesignationAxis_f0b3ce2b-c112-464b-9d77-a731cd6d0f67" xlink:to="loc_us-gaap_HedgingDesignationDomain_5cb97730-da30-4d64-865c-ac0ea2f25609" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DesignatedAsHedgingInstrumentMember_447cafa1-de27-415c-8c8f-6ecfd196487e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DesignatedAsHedgingInstrumentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HedgingDesignationDomain_5cb97730-da30-4d64-865c-ac0ea2f25609" xlink:to="loc_us-gaap_DesignatedAsHedgingInstrumentMember_447cafa1-de27-415c-8c8f-6ecfd196487e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NondesignatedMember_a028c864-0a84-4bae-8b0a-243791be1d7e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NondesignatedMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HedgingDesignationDomain_5cb97730-da30-4d64-865c-ac0ea2f25609" xlink:to="loc_us-gaap_NondesignatedMember_a028c864-0a84-4bae-8b0a-243791be1d7e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BalanceSheetLocationAxis_4180e9e3-8df5-40e8-aed2-b40b7ff8c4fc" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_BalanceSheetLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeTable_74da6d7b-d12e-4bc2-904b-6ef85d4d6816" xlink:to="loc_us-gaap_BalanceSheetLocationAxis_4180e9e3-8df5-40e8-aed2-b40b7ff8c4fc" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BalanceSheetLocationDomain_4180e9e3-8df5-40e8-aed2-b40b7ff8c4fc_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_BalanceSheetLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BalanceSheetLocationAxis_4180e9e3-8df5-40e8-aed2-b40b7ff8c4fc" xlink:to="loc_us-gaap_BalanceSheetLocationDomain_4180e9e3-8df5-40e8-aed2-b40b7ff8c4fc_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BalanceSheetLocationDomain_e593dec9-18aa-4250-a7c2-b7260fe40999" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_BalanceSheetLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BalanceSheetLocationAxis_4180e9e3-8df5-40e8-aed2-b40b7ff8c4fc" xlink:to="loc_us-gaap_BalanceSheetLocationDomain_e593dec9-18aa-4250-a7c2-b7260fe40999" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsMember_3d83d7cb-3047-4720-847b-191405122f64" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BalanceSheetLocationDomain_e593dec9-18aa-4250-a7c2-b7260fe40999" xlink:to="loc_us-gaap_OtherAssetsMember_3d83d7cb-3047-4720-847b-191405122f64" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilitiesMember_d59f54ce-31d0-497a-b3f9-69710a929007" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherLiabilitiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BalanceSheetLocationDomain_e593dec9-18aa-4250-a7c2-b7260fe40999" xlink:to="loc_us-gaap_OtherLiabilitiesMember_d59f54ce-31d0-497a-b3f9-69710a929007" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubordinatedDebtMember_675c1565-5707-495b-90c3-c8e987192eee" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SubordinatedDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BalanceSheetLocationDomain_e593dec9-18aa-4250-a7c2-b7260fe40999" xlink:to="loc_us-gaap_SubordinatedDebtMember_675c1565-5707-495b-90c3-c8e987192eee" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationAxis_8cf0dcff-0a69-487d-85c9-47eeb3d453d9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeStatementLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeTable_74da6d7b-d12e-4bc2-904b-6ef85d4d6816" xlink:to="loc_us-gaap_IncomeStatementLocationAxis_8cf0dcff-0a69-487d-85c9-47eeb3d453d9" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_8cf0dcff-0a69-487d-85c9-47eeb3d453d9_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_8cf0dcff-0a69-487d-85c9-47eeb3d453d9" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_8cf0dcff-0a69-487d-85c9-47eeb3d453d9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_53e59978-1716-4639-a76e-74ae37dce15a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_8cf0dcff-0a69-487d-85c9-47eeb3d453d9" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_53e59978-1716-4639-a76e-74ae37dce15a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseMember_b7e748b3-e5d6-474b-adb8-6e9c5f4b998b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_53e59978-1716-4639-a76e-74ae37dce15a" xlink:to="loc_us-gaap_InterestExpenseMember_b7e748b3-e5d6-474b-adb8-6e9c5f4b998b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestIncomeMember_58ace988-2bb2-4e88-a71e-a077dcaff646" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_53e59978-1716-4639-a76e-74ae37dce15a" xlink:to="loc_us-gaap_InterestIncomeMember_58ace988-2bb2-4e88-a71e-a077dcaff646" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeInstrumentRiskAxis_ebf944ab-082c-47ee-8a01-8f553316c6d1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeInstrumentRiskAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeTable_74da6d7b-d12e-4bc2-904b-6ef85d4d6816" xlink:to="loc_us-gaap_DerivativeInstrumentRiskAxis_ebf944ab-082c-47ee-8a01-8f553316c6d1" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_ebf944ab-082c-47ee-8a01-8f553316c6d1_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_ebf944ab-082c-47ee-8a01-8f553316c6d1" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_ebf944ab-082c-47ee-8a01-8f553316c6d1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_60f38f26-11d4-40a5-8456-e7c6c8a3b2bd" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_ebf944ab-082c-47ee-8a01-8f553316c6d1" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_60f38f26-11d4-40a5-8456-e7c6c8a3b2bd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestRateContractMember_0770f8a2-4d20-4d94-bd7e-5c21a9579bef" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestRateContractMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeContractTypeDomain_60f38f26-11d4-40a5-8456-e7c6c8a3b2bd" xlink:to="loc_us-gaap_InterestRateContractMember_0770f8a2-4d20-4d94-bd7e-5c21a9579bef" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestRateSwapMember_8588efd3-1cd8-41e3-beb7-6b2ea8dacb69" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestRateSwapMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeContractTypeDomain_60f38f26-11d4-40a5-8456-e7c6c8a3b2bd" xlink:to="loc_us-gaap_InterestRateSwapMember_8588efd3-1cd8-41e3-beb7-6b2ea8dacb69" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_InterestRateCollarMember_0d7ba457-a63d-4e8f-9887-bf63fb7518ca" xlink:href="efsc-20221231.xsd#efsc_InterestRateCollarMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeContractTypeDomain_60f38f26-11d4-40a5-8456-e7c6c8a3b2bd" xlink:to="loc_efsc_InterestRateCollarMember_0d7ba457-a63d-4e8f-9887-bf63fb7518ca" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeAxis_84c0e22f-9db6-4bb3-877e-1e39bb3baf62" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SubsequentEventTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeTable_74da6d7b-d12e-4bc2-904b-6ef85d4d6816" xlink:to="loc_us-gaap_SubsequentEventTypeAxis_84c0e22f-9db6-4bb3-877e-1e39bb3baf62" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_84c0e22f-9db6-4bb3-877e-1e39bb3baf62_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_84c0e22f-9db6-4bb3-877e-1e39bb3baf62" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_84c0e22f-9db6-4bb3-877e-1e39bb3baf62_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_d47c6cac-798e-45ee-b34c-f47e9cc161cf" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_84c0e22f-9db6-4bb3-877e-1e39bb3baf62" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_d47c6cac-798e-45ee-b34c-f47e9cc161cf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventMember_c999dc3e-92fa-40ca-8458-ddf7c767db96" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SubsequentEventMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventTypeDomain_d47c6cac-798e-45ee-b34c-f47e9cc161cf" xlink:to="loc_us-gaap_SubsequentEventMember_c999dc3e-92fa-40ca-8458-ddf7c767db96" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_18fcad58-c7d1-4ecf-a24e-a4579f2b1961" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeTable_74da6d7b-d12e-4bc2-904b-6ef85d4d6816" xlink:to="loc_srt_RangeAxis_18fcad58-c7d1-4ecf-a24e-a4579f2b1961" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_18fcad58-c7d1-4ecf-a24e-a4579f2b1961_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_18fcad58-c7d1-4ecf-a24e-a4579f2b1961" xlink:to="loc_srt_RangeMember_18fcad58-c7d1-4ecf-a24e-a4579f2b1961_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_ea76dfbe-8b45-4537-90e1-46061005ee19" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_18fcad58-c7d1-4ecf-a24e-a4579f2b1961" xlink:to="loc_srt_RangeMember_ea76dfbe-8b45-4537-90e1-46061005ee19" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_b4eb65d7-3fdc-411a-81aa-81f0f140e26c" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_ea76dfbe-8b45-4537-90e1-46061005ee19" xlink:to="loc_srt_MinimumMember_b4eb65d7-3fdc-411a-81aa-81f0f140e26c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_d69d6934-46da-4256-810a-c88e4c82439c" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_ea76dfbe-8b45-4537-90e1-46061005ee19" xlink:to="loc_srt_MaximumMember_d69d6934-46da-4256-810a-c88e4c82439c" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/DerivativeFinancialInstrumentsCashFlowHedgesDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#DerivativeFinancialInstrumentsCashFlowHedgesDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/DerivativeFinancialInstrumentsCashFlowHedgesDetails" xlink:type="extended" id="i2bd21c9b86b24fe0b220cb618cf2caef_DerivativeFinancialInstrumentsCashFlowHedgesDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeLineItems_7eb6c9d1-40b2-4425-ad27-864a27cd2d75" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeAssetNotionalAmount_54c3c928-7fc3-47a6-ba71-6da793f17ea7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeAssetNotionalAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_7eb6c9d1-40b2-4425-ad27-864a27cd2d75" xlink:to="loc_us-gaap_DerivativeAssetNotionalAmount_54c3c928-7fc3-47a6-ba71-6da793f17ea7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeFixedInterestRate_b376e499-e1fb-4c91-a996-942d0915dd13" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeFixedInterestRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_7eb6c9d1-40b2-4425-ad27-864a27cd2d75" xlink:to="loc_us-gaap_DerivativeFixedInterestRate_b376e499-e1fb-4c91-a996-942d0915dd13" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeTable_c427df4f-a104-4ebb-80b4-7a507e5e5e2a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DerivativeLineItems_7eb6c9d1-40b2-4425-ad27-864a27cd2d75" xlink:to="loc_us-gaap_DerivativeTable_c427df4f-a104-4ebb-80b4-7a507e5e5e2a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeInstrumentRiskAxis_1b4bb263-6678-4c29-9ce0-4c1a64f8f46b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeInstrumentRiskAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeTable_c427df4f-a104-4ebb-80b4-7a507e5e5e2a" xlink:to="loc_us-gaap_DerivativeInstrumentRiskAxis_1b4bb263-6678-4c29-9ce0-4c1a64f8f46b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_1b4bb263-6678-4c29-9ce0-4c1a64f8f46b_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_1b4bb263-6678-4c29-9ce0-4c1a64f8f46b" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_1b4bb263-6678-4c29-9ce0-4c1a64f8f46b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_f9137391-3892-45e3-9c12-9acc8c21a368" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_1b4bb263-6678-4c29-9ce0-4c1a64f8f46b" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_f9137391-3892-45e3-9c12-9acc8c21a368" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CashFlowHedge1Member_0bdc2316-d8f0-4e69-9e5a-0aba212a1b54" xlink:href="efsc-20221231.xsd#efsc_CashFlowHedge1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeContractTypeDomain_f9137391-3892-45e3-9c12-9acc8c21a368" xlink:to="loc_efsc_CashFlowHedge1Member_0bdc2316-d8f0-4e69-9e5a-0aba212a1b54" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CashFlowHedge2Member_9655eb4f-c5d8-4b99-974e-9f7b8dcc0aab" xlink:href="efsc-20221231.xsd#efsc_CashFlowHedge2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeContractTypeDomain_f9137391-3892-45e3-9c12-9acc8c21a368" xlink:to="loc_efsc_CashFlowHedge2Member_9655eb4f-c5d8-4b99-974e-9f7b8dcc0aab" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CashFlowHedge3Member_8dd56481-b9c4-47e2-86b3-a2511334b617" xlink:href="efsc-20221231.xsd#efsc_CashFlowHedge3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeContractTypeDomain_f9137391-3892-45e3-9c12-9acc8c21a368" xlink:to="loc_efsc_CashFlowHedge3Member_8dd56481-b9c4-47e2-86b3-a2511334b617" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CashFlowHedge4Member_6b7ba7c7-6a2d-47d7-91d7-2964071fff49" xlink:href="efsc-20221231.xsd#efsc_CashFlowHedge4Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeContractTypeDomain_f9137391-3892-45e3-9c12-9acc8c21a368" xlink:to="loc_efsc_CashFlowHedge4Member_6b7ba7c7-6a2d-47d7-91d7-2964071fff49" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeInstrumentsGainLossByHedgingRelationshipAxis_aa7f0b6b-8908-41ab-bdb6-eb0d433252b8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeInstrumentsGainLossByHedgingRelationshipAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeTable_c427df4f-a104-4ebb-80b4-7a507e5e5e2a" xlink:to="loc_us-gaap_DerivativeInstrumentsGainLossByHedgingRelationshipAxis_aa7f0b6b-8908-41ab-bdb6-eb0d433252b8" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HedgingRelationshipDomain_aa7f0b6b-8908-41ab-bdb6-eb0d433252b8_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_HedgingRelationshipDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DerivativeInstrumentsGainLossByHedgingRelationshipAxis_aa7f0b6b-8908-41ab-bdb6-eb0d433252b8" xlink:to="loc_us-gaap_HedgingRelationshipDomain_aa7f0b6b-8908-41ab-bdb6-eb0d433252b8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HedgingRelationshipDomain_c3d738f7-b556-4664-bab3-a2e8ce86e678" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_HedgingRelationshipDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DerivativeInstrumentsGainLossByHedgingRelationshipAxis_aa7f0b6b-8908-41ab-bdb6-eb0d433252b8" xlink:to="loc_us-gaap_HedgingRelationshipDomain_c3d738f7-b556-4664-bab3-a2e8ce86e678" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashFlowHedgingMember_9cc57008-abd0-4b86-838b-c6400fa482fc" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CashFlowHedgingMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HedgingRelationshipDomain_c3d738f7-b556-4664-bab3-a2e8ce86e678" xlink:to="loc_us-gaap_CashFlowHedgingMember_9cc57008-abd0-4b86-838b-c6400fa482fc" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/FixedAssetsNarrativeDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#FixedAssetsNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/FixedAssetsNarrativeDetails" xlink:type="extended" id="ie541beb03eb740b59de02fae777580be_FixedAssetsNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentLineItems_a0018319-d4ff-4e67-8b40-404a732180c0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepreciationDepletionAndAmortization_d6f48bc4-cf77-4057-94dd-c5c97989615d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DepreciationDepletionAndAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_a0018319-d4ff-4e67-8b40-404a732180c0" xlink:to="loc_us-gaap_DepreciationDepletionAndAmortization_d6f48bc4-cf77-4057-94dd-c5c97989615d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_NumberOfBranchesClosed_6f1b03aa-a374-4221-a917-20dc6a7f1a35" xlink:href="efsc-20221231.xsd#efsc_NumberOfBranchesClosed"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_a0018319-d4ff-4e67-8b40-404a732180c0" xlink:to="loc_efsc_NumberOfBranchesClosed_6f1b03aa-a374-4221-a917-20dc6a7f1a35" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetImpairmentCharges_93e59103-9ad9-4cc7-884f-520143653983" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AssetImpairmentCharges"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_a0018319-d4ff-4e67-8b40-404a732180c0" xlink:to="loc_us-gaap_AssetImpairmentCharges_93e59103-9ad9-4cc7-884f-520143653983" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_5ef5b1ee-6311-4c30-8ce5-81db9c50f273" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_a0018319-d4ff-4e67-8b40-404a732180c0" xlink:to="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_5ef5b1ee-6311-4c30-8ce5-81db9c50f273" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_c073a3a0-3ed9-4587-bf27-f18d0e02bc32" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_5ef5b1ee-6311-4c30-8ce5-81db9c50f273" xlink:to="loc_srt_StatementGeographicalAxis_c073a3a0-3ed9-4587-bf27-f18d0e02bc32" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_c073a3a0-3ed9-4587-bf27-f18d0e02bc32_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_c073a3a0-3ed9-4587-bf27-f18d0e02bc32" xlink:to="loc_srt_SegmentGeographicalDomain_c073a3a0-3ed9-4587-bf27-f18d0e02bc32_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_86813a42-3074-4c14-8b17-e596dc30e480" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_c073a3a0-3ed9-4587-bf27-f18d0e02bc32" xlink:to="loc_srt_SegmentGeographicalDomain_86813a42-3074-4c14-8b17-e596dc30e480" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_stpr_CA_a07aae0d-d163-421f-82ef-568c8e331ada" xlink:href="https://xbrl.sec.gov/stpr/2021/stpr-2021.xsd#stpr_CA"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_86813a42-3074-4c14-8b17-e596dc30e480" xlink:to="loc_stpr_CA_a07aae0d-d163-421f-82ef-568c8e331ada" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_StLouisMember_7d465bbc-d516-4068-adbc-42681848f4fc" xlink:href="efsc-20221231.xsd#efsc_StLouisMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_86813a42-3074-4c14-8b17-e596dc30e480" xlink:to="loc_efsc_StLouisMember_7d465bbc-d516-4068-adbc-42681848f4fc" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/GoodwillandIntangibleAssetsDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#GoodwillandIntangibleAssetsDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/GoodwillandIntangibleAssetsDetails" xlink:type="extended" id="i05dbdd98bf96475ea67b6cbc4de5d2d9_GoodwillandIntangibleAssetsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_c9fb2d1c-460f-4459-bb0e-25a104412125" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AcquiredFiniteLivedIntangibleAssetResidualValue_e3d8061b-4844-41f8-a7cf-1a9034cb3e16" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AcquiredFiniteLivedIntangibleAssetResidualValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_c9fb2d1c-460f-4459-bb0e-25a104412125" xlink:to="loc_us-gaap_AcquiredFiniteLivedIntangibleAssetResidualValue_e3d8061b-4844-41f8-a7cf-1a9034cb3e16" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_0f528eb9-0b73-4f0c-be0c-b69cb871d0d2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_c9fb2d1c-460f-4459-bb0e-25a104412125" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_0f528eb9-0b73-4f0c-be0c-b69cb871d0d2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsNet_1ec43787-2b25-40c1-8e16-df1cc2ba3e3b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_c9fb2d1c-460f-4459-bb0e-25a104412125" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsNet_1ec43787-2b25-40c1-8e16-df1cc2ba3e3b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Goodwill_26682209-1442-40d6-8619-31a99f608516" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_Goodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_c9fb2d1c-460f-4459-bb0e-25a104412125" xlink:to="loc_us-gaap_Goodwill_26682209-1442-40d6-8619-31a99f608516" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_GoodwillAcquiredThroughAcquisition_f6bac0a2-10e8-463d-bd3a-2c0cf2f4057b" xlink:href="efsc-20221231.xsd#efsc_GoodwillAcquiredThroughAcquisition"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_c9fb2d1c-460f-4459-bb0e-25a104412125" xlink:to="loc_efsc_GoodwillAcquiredThroughAcquisition_f6bac0a2-10e8-463d-bd3a-2c0cf2f4057b" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_572a4693-5714-4b27-b968-0cbd32df704f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_c9fb2d1c-460f-4459-bb0e-25a104412125" xlink:to="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_572a4693-5714-4b27-b968-0cbd32df704f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_4ebc7008-150f-4cc4-bbd4-5370fdb6c2e6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_572a4693-5714-4b27-b968-0cbd32df704f" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_4ebc7008-150f-4cc4-bbd4-5370fdb6c2e6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_4ebc7008-150f-4cc4-bbd4-5370fdb6c2e6_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_4ebc7008-150f-4cc4-bbd4-5370fdb6c2e6" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_4ebc7008-150f-4cc4-bbd4-5370fdb6c2e6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_b267bd7b-8219-4384-aaf0-f6bf49791c5f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_4ebc7008-150f-4cc4-bbd4-5370fdb6c2e6" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_b267bd7b-8219-4384-aaf0-f6bf49791c5f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CoreDepositsMember_9ef342ee-152e-4a21-a2a2-9561d3e0c837" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CoreDepositsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_b267bd7b-8219-4384-aaf0-f6bf49791c5f" xlink:to="loc_us-gaap_CoreDepositsMember_9ef342ee-152e-4a21-a2a2-9561d3e0c837" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/SubordinatedDebenturesDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#SubordinatedDebenturesDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/SubordinatedDebenturesDetails" xlink:type="extended" id="i63c1990b153f4988b605c51cd89d66c3_SubordinatedDebenturesDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubordinatedBorrowingLineItems_65ff2ffe-4055-4a4b-bbd5-0512169062a7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SubordinatedBorrowingLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_NumberofUnconsolidatedStatutoryBusinessTrusts_c66b6269-e4c9-4a5b-bf81-10bc081be709" xlink:href="efsc-20221231.xsd#efsc_NumberofUnconsolidatedStatutoryBusinessTrusts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubordinatedBorrowingLineItems_65ff2ffe-4055-4a4b-bbd5-0512169062a7" xlink:to="loc_efsc_NumberofUnconsolidatedStatutoryBusinessTrusts_c66b6269-e4c9-4a5b-bf81-10bc081be709" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_Investmentstrustpreferredsecurities_d9563bed-9d7d-416b-b343-2be4abf44e81" xlink:href="efsc-20221231.xsd#efsc_Investmentstrustpreferredsecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubordinatedBorrowingLineItems_65ff2ffe-4055-4a4b-bbd5-0512169062a7" xlink:to="loc_efsc_Investmentstrustpreferredsecurities_d9563bed-9d7d-416b-b343-2be4abf44e81" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_JuniorSubordinatedNotes_a9e3d64c-0a23-467c-9d76-6084f35c41bd" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_JuniorSubordinatedNotes"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubordinatedBorrowingLineItems_65ff2ffe-4055-4a4b-bbd5-0512169062a7" xlink:to="loc_us-gaap_JuniorSubordinatedNotes_a9e3d64c-0a23-467c-9d76-6084f35c41bd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubordinatedBorrowingInterestRate_cf6b7d8f-1b4d-4227-b09e-0ffd328e17cf" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SubordinatedBorrowingInterestRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubordinatedBorrowingLineItems_65ff2ffe-4055-4a4b-bbd5-0512169062a7" xlink:to="loc_us-gaap_SubordinatedBorrowingInterestRate_cf6b7d8f-1b4d-4227-b09e-0ffd328e17cf" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_JuniorSubordinatedNotesRedeemedValue_cb95b768-68a3-4ac6-9ba3-ff68976f6c2a" xlink:href="efsc-20221231.xsd#efsc_JuniorSubordinatedNotesRedeemedValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubordinatedBorrowingLineItems_65ff2ffe-4055-4a4b-bbd5-0512169062a7" xlink:to="loc_efsc_JuniorSubordinatedNotesRedeemedValue_cb95b768-68a3-4ac6-9ba3-ff68976f6c2a" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_JuniorSubordinatedNotesGainLossOnRedemption_2b74131b-e4cb-4c98-a71e-0c11cc2ef105" xlink:href="efsc-20221231.xsd#efsc_JuniorSubordinatedNotesGainLossOnRedemption"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubordinatedBorrowingLineItems_65ff2ffe-4055-4a4b-bbd5-0512169062a7" xlink:to="loc_efsc_JuniorSubordinatedNotesGainLossOnRedemption_2b74131b-e4cb-4c98-a71e-0c11cc2ef105" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubordinatedDebt_89b39a80-ddc6-4e7d-9d74-124c641a59dc" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SubordinatedDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubordinatedBorrowingLineItems_65ff2ffe-4055-4a4b-bbd5-0512169062a7" xlink:to="loc_us-gaap_SubordinatedDebt_89b39a80-ddc6-4e7d-9d74-124c641a59dc" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsOfDebtIssuanceCosts_b5047c83-c00c-48f3-86ea-00a7533c90c8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PaymentsOfDebtIssuanceCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubordinatedBorrowingLineItems_65ff2ffe-4055-4a4b-bbd5-0512169062a7" xlink:to="loc_us-gaap_PaymentsOfDebtIssuanceCosts_b5047c83-c00c-48f3-86ea-00a7533c90c8" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_Subordinatednotesnetofissuancecosts_105198ec-cab9-4f28-a161-0a65eace43d7" xlink:href="efsc-20221231.xsd#efsc_Subordinatednotesnetofissuancecosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubordinatedBorrowingLineItems_65ff2ffe-4055-4a4b-bbd5-0512169062a7" xlink:to="loc_efsc_Subordinatednotesnetofissuancecosts_105198ec-cab9-4f28-a161-0a65eace43d7" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubordinatedBorrowingDueDate_00960861-2c8b-422a-b339-b2b307416548" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SubordinatedBorrowingDueDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubordinatedBorrowingLineItems_65ff2ffe-4055-4a4b-bbd5-0512169062a7" xlink:to="loc_us-gaap_SubordinatedBorrowingDueDate_00960861-2c8b-422a-b339-b2b307416548" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_SubordinatedBorrowingCallDate_29e46b2f-e1d7-432f-84a2-cad3fdf7cb16" xlink:href="efsc-20221231.xsd#efsc_SubordinatedBorrowingCallDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubordinatedBorrowingLineItems_65ff2ffe-4055-4a4b-bbd5-0512169062a7" xlink:to="loc_efsc_SubordinatedBorrowingCallDate_29e46b2f-e1d7-432f-84a2-cad3fdf7cb16" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_SubordinatedBorrowingBasisSpreadonVariableRate_2aa20bcf-487e-4e00-b751-2c75c4bb27a1" xlink:href="efsc-20221231.xsd#efsc_SubordinatedBorrowingBasisSpreadonVariableRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubordinatedBorrowingLineItems_65ff2ffe-4055-4a4b-bbd5-0512169062a7" xlink:to="loc_efsc_SubordinatedBorrowingBasisSpreadonVariableRate_2aa20bcf-487e-4e00-b751-2c75c4bb27a1" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubordinatedBorrowingTable_38f2bb9a-4864-445a-9535-fecab9e73a66" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SubordinatedBorrowingTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SubordinatedBorrowingLineItems_65ff2ffe-4055-4a4b-bbd5-0512169062a7" xlink:to="loc_us-gaap_SubordinatedBorrowingTable_38f2bb9a-4864-445a-9535-fecab9e73a66" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_da9c87c1-ea7d-41a2-8234-b86c58dda4fa" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubordinatedBorrowingTable_38f2bb9a-4864-445a-9535-fecab9e73a66" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_da9c87c1-ea7d-41a2-8234-b86c58dda4fa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_da9c87c1-ea7d-41a2-8234-b86c58dda4fa_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_da9c87c1-ea7d-41a2-8234-b86c58dda4fa" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_da9c87c1-ea7d-41a2-8234-b86c58dda4fa_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_51fdbfb0-7f38-4444-aadc-b9c0f5838d52" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_da9c87c1-ea7d-41a2-8234-b86c58dda4fa" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_51fdbfb0-7f38-4444-aadc-b9c0f5838d52" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TrustPreferredSecuritiesSubjectToMandatoryRedemptionMember_aa454be9-d281-41a8-b1a5-f97303c2dfae" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TrustPreferredSecuritiesSubjectToMandatoryRedemptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_51fdbfb0-7f38-4444-aadc-b9c0f5838d52" xlink:to="loc_us-gaap_TrustPreferredSecuritiesSubjectToMandatoryRedemptionMember_aa454be9-d281-41a8-b1a5-f97303c2dfae" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeniorSubordinatedNotesMember_8bebd65d-ff45-4790-8070-2cc91a323829" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SeniorSubordinatedNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_51fdbfb0-7f38-4444-aadc-b9c0f5838d52" xlink:to="loc_us-gaap_SeniorSubordinatedNotesMember_8bebd65d-ff45-4790-8070-2cc91a323829" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubordinatedBorrowingAxis_b5fcc16b-f898-44c5-a060-dddcb705631e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SubordinatedBorrowingAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubordinatedBorrowingTable_38f2bb9a-4864-445a-9535-fecab9e73a66" xlink:to="loc_us-gaap_SubordinatedBorrowingAxis_b5fcc16b-f898-44c5-a060-dddcb705631e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubordinatedBorrowingNameDomain_b5fcc16b-f898-44c5-a060-dddcb705631e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SubordinatedBorrowingNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubordinatedBorrowingAxis_b5fcc16b-f898-44c5-a060-dddcb705631e" xlink:to="loc_us-gaap_SubordinatedBorrowingNameDomain_b5fcc16b-f898-44c5-a060-dddcb705631e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubordinatedBorrowingNameDomain_babecb8e-40d2-473c-9a6c-22e9644e914a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SubordinatedBorrowingNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubordinatedBorrowingAxis_b5fcc16b-f898-44c5-a060-dddcb705631e" xlink:to="loc_us-gaap_SubordinatedBorrowingNameDomain_babecb8e-40d2-473c-9a6c-22e9644e914a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeniorSubordinatedNotesMember_e4675091-7e9c-4602-961f-bc4f1f404117" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SeniorSubordinatedNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubordinatedBorrowingNameDomain_babecb8e-40d2-473c-9a6c-22e9644e914a" xlink:to="loc_us-gaap_SeniorSubordinatedNotesMember_e4675091-7e9c-4602-961f-bc4f1f404117" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_EfscClaycoStatutoryTrustIMember_ffb4c28b-30fa-401a-8690-3cb3b557406c" xlink:href="efsc-20221231.xsd#efsc_EfscClaycoStatutoryTrustIMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubordinatedBorrowingNameDomain_babecb8e-40d2-473c-9a6c-22e9644e914a" xlink:to="loc_efsc_EfscClaycoStatutoryTrustIMember_ffb4c28b-30fa-401a-8690-3cb3b557406c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_EfscCapitalTrustIiMember_ce8812d7-071d-4736-a795-8cbad846d9e1" xlink:href="efsc-20221231.xsd#efsc_EfscCapitalTrustIiMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubordinatedBorrowingNameDomain_babecb8e-40d2-473c-9a6c-22e9644e914a" xlink:to="loc_efsc_EfscCapitalTrustIiMember_ce8812d7-071d-4736-a795-8cbad846d9e1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_EfscStatutoryTrustIiiMember_8ee97689-ef33-420f-b6fc-0b292e4151e5" xlink:href="efsc-20221231.xsd#efsc_EfscStatutoryTrustIiiMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubordinatedBorrowingNameDomain_babecb8e-40d2-473c-9a6c-22e9644e914a" xlink:to="loc_efsc_EfscStatutoryTrustIiiMember_8ee97689-ef33-420f-b6fc-0b292e4151e5" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_EfscClaycoStatutoryTrustIiMember_3379a991-c78d-4fd6-bacc-be3ac7ed9525" xlink:href="efsc-20221231.xsd#efsc_EfscClaycoStatutoryTrustIiMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubordinatedBorrowingNameDomain_babecb8e-40d2-473c-9a6c-22e9644e914a" xlink:to="loc_efsc_EfscClaycoStatutoryTrustIiMember_3379a991-c78d-4fd6-bacc-be3ac7ed9525" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_EfscStatutoryTrustIvMember_89783d79-c846-46cf-8a52-ba6e74fc1ec6" xlink:href="efsc-20221231.xsd#efsc_EfscStatutoryTrustIvMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubordinatedBorrowingNameDomain_babecb8e-40d2-473c-9a6c-22e9644e914a" xlink:to="loc_efsc_EfscStatutoryTrustIvMember_89783d79-c846-46cf-8a52-ba6e74fc1ec6" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_EfscStatutoryTrustVMember_9079f417-1cd4-48e0-b6fa-94cb7076ec5d" xlink:href="efsc-20221231.xsd#efsc_EfscStatutoryTrustVMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubordinatedBorrowingNameDomain_babecb8e-40d2-473c-9a6c-22e9644e914a" xlink:to="loc_efsc_EfscStatutoryTrustVMember_9079f417-1cd4-48e0-b6fa-94cb7076ec5d" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_EfscCapitalTrustViMember_af35e3cb-7c75-4259-9326-cbeb416a46c6" xlink:href="efsc-20221231.xsd#efsc_EfscCapitalTrustViMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubordinatedBorrowingNameDomain_babecb8e-40d2-473c-9a6c-22e9644e914a" xlink:to="loc_efsc_EfscCapitalTrustViMember_af35e3cb-7c75-4259-9326-cbeb416a46c6" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_EfscCapitalTrustViiMember_8fe245bf-1c04-4cb2-a46c-d60c791b7a70" xlink:href="efsc-20221231.xsd#efsc_EfscCapitalTrustViiMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubordinatedBorrowingNameDomain_babecb8e-40d2-473c-9a6c-22e9644e914a" xlink:to="loc_efsc_EfscCapitalTrustViiMember_8fe245bf-1c04-4cb2-a46c-d60c791b7a70" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_JEFFCOStatTrustIMember_67aea7ac-45c2-4951-a035-a81b6f52310d" xlink:href="efsc-20221231.xsd#efsc_JEFFCOStatTrustIMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubordinatedBorrowingNameDomain_babecb8e-40d2-473c-9a6c-22e9644e914a" xlink:to="loc_efsc_JEFFCOStatTrustIMember_67aea7ac-45c2-4951-a035-a81b6f52310d" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_JEFFCOStatTrustIIMember_8a27109e-be37-46c0-a452-57347af1558b" xlink:href="efsc-20221231.xsd#efsc_JEFFCOStatTrustIIMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubordinatedBorrowingNameDomain_babecb8e-40d2-473c-9a6c-22e9644e914a" xlink:to="loc_efsc_JEFFCOStatTrustIIMember_8a27109e-be37-46c0-a452-57347af1558b" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_TrinityCapitalTrustIIIMember_70d59dfa-7eaa-46c6-afe5-2bbd986a190b" xlink:href="efsc-20221231.xsd#efsc_TrinityCapitalTrustIIIMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubordinatedBorrowingNameDomain_babecb8e-40d2-473c-9a6c-22e9644e914a" xlink:to="loc_efsc_TrinityCapitalTrustIIIMember_70d59dfa-7eaa-46c6-afe5-2bbd986a190b" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_TrinityCapitalTrustIVMember_99813e19-6dfe-4494-b84d-c1ff1fc4d37a" xlink:href="efsc-20221231.xsd#efsc_TrinityCapitalTrustIVMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubordinatedBorrowingNameDomain_babecb8e-40d2-473c-9a6c-22e9644e914a" xlink:to="loc_efsc_TrinityCapitalTrustIVMember_99813e19-6dfe-4494-b84d-c1ff1fc4d37a" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_TrinityCapitalTrustVMember_3440000f-abc1-4601-b9a2-d48008a533d2" xlink:href="efsc-20221231.xsd#efsc_TrinityCapitalTrustVMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubordinatedBorrowingNameDomain_babecb8e-40d2-473c-9a6c-22e9644e914a" xlink:to="loc_efsc_TrinityCapitalTrustVMember_3440000f-abc1-4601-b9a2-d48008a533d2" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_FixedInterestRateAxis_6b8facc7-d2ae-4ae7-b145-8a2ad9903a7b" xlink:href="efsc-20221231.xsd#efsc_FixedInterestRateAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubordinatedBorrowingTable_38f2bb9a-4864-445a-9535-fecab9e73a66" xlink:to="loc_efsc_FixedInterestRateAxis_6b8facc7-d2ae-4ae7-b145-8a2ad9903a7b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_FixedRateDomain_6b8facc7-d2ae-4ae7-b145-8a2ad9903a7b_default" xlink:href="efsc-20221231.xsd#efsc_FixedRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_efsc_FixedInterestRateAxis_6b8facc7-d2ae-4ae7-b145-8a2ad9903a7b" xlink:to="loc_efsc_FixedRateDomain_6b8facc7-d2ae-4ae7-b145-8a2ad9903a7b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_FixedRateDomain_062c373d-fb3d-4b9a-8a04-294b9c21f248" xlink:href="efsc-20221231.xsd#efsc_FixedRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_efsc_FixedInterestRateAxis_6b8facc7-d2ae-4ae7-b145-8a2ad9903a7b" xlink:to="loc_efsc_FixedRateDomain_062c373d-fb3d-4b9a-8a04-294b9c21f248" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_A575Member_b9392498-897b-4c55-8feb-4bae2a20d3b7" xlink:href="efsc-20221231.xsd#efsc_A575Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_efsc_FixedRateDomain_062c373d-fb3d-4b9a-8a04-294b9c21f248" xlink:to="loc_efsc_A575Member_b9392498-897b-4c55-8feb-4bae2a20d3b7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateAxis_89251b49-f3ef-4a82-8f9d-98b0e31bf6d9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_VariableRateAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubordinatedBorrowingTable_38f2bb9a-4864-445a-9535-fecab9e73a66" xlink:to="loc_us-gaap_VariableRateAxis_89251b49-f3ef-4a82-8f9d-98b0e31bf6d9" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_89251b49-f3ef-4a82-8f9d-98b0e31bf6d9_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_VariableRateAxis_89251b49-f3ef-4a82-8f9d-98b0e31bf6d9" xlink:to="loc_us-gaap_VariableRateDomain_89251b49-f3ef-4a82-8f9d-98b0e31bf6d9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_5d766e9c-9519-476a-b495-68817d2d7cce" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_VariableRateAxis_89251b49-f3ef-4a82-8f9d-98b0e31bf6d9" xlink:to="loc_us-gaap_VariableRateDomain_5d766e9c-9519-476a-b495-68817d2d7cce" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuredOvernightFinancingRateSofrOvernightIndexSwapRateMember_a3905eb0-e2ab-4eba-8f5e-50c832524c2a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SecuredOvernightFinancingRateSofrOvernightIndexSwapRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableRateDomain_5d766e9c-9519-476a-b495-68817d2d7cce" xlink:to="loc_us-gaap_SecuredOvernightFinancingRateSofrOvernightIndexSwapRateMember_a3905eb0-e2ab-4eba-8f5e-50c832524c2a" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/FederalHomeLoanBankAdvancesDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#FederalHomeLoanBankAdvancesDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/FederalHomeLoanBankAdvancesDetails" xlink:type="extended" id="id6f1aedb3c4d4574be9f9bcef208e0c6_FederalHomeLoanBankAdvancesDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FederalHomeLoanBankAdvancesBranchOfFHLBBankLineItems_d3905c20-680d-40a0-82f7-63cac073f4f0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FederalHomeLoanBankAdvancesBranchOfFHLBBankLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FederalHomeLoanBankAdvances_321dd884-cd7a-4431-99bc-537792992ca4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FederalHomeLoanBankAdvances"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FederalHomeLoanBankAdvancesBranchOfFHLBBankLineItems_d3905c20-680d-40a0-82f7-63cac073f4f0" xlink:to="loc_us-gaap_FederalHomeLoanBankAdvances_321dd884-cd7a-4431-99bc-537792992ca4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FederalHomeLoanBankAdvancesGeneralDebtObligationsDisclosuresAmountOfAvailableUnusedFunds_66d6cc4b-64ac-4a7a-9b60-70ccdd890c5c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FederalHomeLoanBankAdvancesGeneralDebtObligationsDisclosuresAmountOfAvailableUnusedFunds"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FederalHomeLoanBankAdvancesBranchOfFHLBBankLineItems_d3905c20-680d-40a0-82f7-63cac073f4f0" xlink:to="loc_us-gaap_FederalHomeLoanBankAdvancesGeneralDebtObligationsDisclosuresAmountOfAvailableUnusedFunds_66d6cc4b-64ac-4a7a-9b60-70ccdd890c5c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_FederalHomeLoanBankAdvancesMaturityOneWeek_02b36471-e04f-4b4c-bf72-f5477c990c8e" xlink:href="efsc-20221231.xsd#efsc_FederalHomeLoanBankAdvancesMaturityOneWeek"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FederalHomeLoanBankAdvancesBranchOfFHLBBankLineItems_d3905c20-680d-40a0-82f7-63cac073f4f0" xlink:to="loc_efsc_FederalHomeLoanBankAdvancesMaturityOneWeek_02b36471-e04f-4b4c-bf72-f5477c990c8e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_FederalHomeLoanBankAdvancesOvernightFunds_5394b73d-954a-4008-9b2b-b74b76bcffb1" xlink:href="efsc-20221231.xsd#efsc_FederalHomeLoanBankAdvancesOvernightFunds"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FederalHomeLoanBankAdvancesBranchOfFHLBBankLineItems_d3905c20-680d-40a0-82f7-63cac073f4f0" xlink:to="loc_efsc_FederalHomeLoanBankAdvancesOvernightFunds_5394b73d-954a-4008-9b2b-b74b76bcffb1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConvertibleDebt_643c4c37-cf7e-4e76-85d4-b7ffb579e5fc" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ConvertibleDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FederalHomeLoanBankAdvancesBranchOfFHLBBankLineItems_d3905c20-680d-40a0-82f7-63cac073f4f0" xlink:to="loc_us-gaap_ConvertibleDebt_643c4c37-cf7e-4e76-85d4-b7ffb579e5fc" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_Convertibleadvancesweightedaverageinterestrate_e6d4e71b-4dea-4174-a0cc-d20eb0719e4c" xlink:href="efsc-20221231.xsd#efsc_Convertibleadvancesweightedaverageinterestrate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FederalHomeLoanBankAdvancesBranchOfFHLBBankLineItems_d3905c20-680d-40a0-82f7-63cac073f4f0" xlink:to="loc_efsc_Convertibleadvancesweightedaverageinterestrate_e6d4e71b-4dea-4174-a0cc-d20eb0719e4c" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FederalHomeLoanBankAdvancesMaturitiesSummaryAverageInterestRateOfAmountsDueAbstract_5b024434-a0a3-4d8e-89c2-29cadd80af8d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FederalHomeLoanBankAdvancesMaturitiesSummaryAverageInterestRateOfAmountsDueAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FederalHomeLoanBankAdvancesBranchOfFHLBBankLineItems_d3905c20-680d-40a0-82f7-63cac073f4f0" xlink:to="loc_us-gaap_FederalHomeLoanBankAdvancesMaturitiesSummaryAverageInterestRateOfAmountsDueAbstract_5b024434-a0a3-4d8e-89c2-29cadd80af8d" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FederalHomeLoanBankAdvancesMaturitiesSummaryByInterestRateTypeOneToFiveYears_98411dbc-c88a-466f-b98a-fb3d28f0c53e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FederalHomeLoanBankAdvancesMaturitiesSummaryByInterestRateTypeOneToFiveYears"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FederalHomeLoanBankAdvancesMaturitiesSummaryAverageInterestRateOfAmountsDueAbstract_5b024434-a0a3-4d8e-89c2-29cadd80af8d" xlink:to="loc_us-gaap_FederalHomeLoanBankAdvancesMaturitiesSummaryByInterestRateTypeOneToFiveYears_98411dbc-c88a-466f-b98a-fb3d28f0c53e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_FederalHomeLoanBankAdvancesMaturitiesSummaryAverageInterestRateofAmountsDueinOnetoFiveYearsofBalanceSheetDate_f938b387-2c54-4347-b2a1-014a94945257" xlink:href="efsc-20221231.xsd#efsc_FederalHomeLoanBankAdvancesMaturitiesSummaryAverageInterestRateofAmountsDueinOnetoFiveYearsofBalanceSheetDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FederalHomeLoanBankAdvancesMaturitiesSummaryAverageInterestRateOfAmountsDueAbstract_5b024434-a0a3-4d8e-89c2-29cadd80af8d" xlink:to="loc_efsc_FederalHomeLoanBankAdvancesMaturitiesSummaryAverageInterestRateofAmountsDueinOnetoFiveYearsofBalanceSheetDate_f938b387-2c54-4347-b2a1-014a94945257" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFederalHomeLoanBankAdvancesByBranchOfFHLBBankTable_58d85e30-00a5-410f-8523-153e573649c1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfFederalHomeLoanBankAdvancesByBranchOfFHLBBankTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FederalHomeLoanBankAdvancesBranchOfFHLBBankLineItems_d3905c20-680d-40a0-82f7-63cac073f4f0" xlink:to="loc_us-gaap_ScheduleOfFederalHomeLoanBankAdvancesByBranchOfFHLBBankTable_58d85e30-00a5-410f-8523-153e573649c1" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_FederalHomeLoanBankAdvancesBranchOfFHLBBankAxis_38af9327-4dd1-4b59-95b9-c7b58ac055c8" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_FederalHomeLoanBankAdvancesBranchOfFHLBBankAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFederalHomeLoanBankAdvancesByBranchOfFHLBBankTable_58d85e30-00a5-410f-8523-153e573649c1" xlink:to="loc_srt_FederalHomeLoanBankAdvancesBranchOfFHLBBankAxis_38af9327-4dd1-4b59-95b9-c7b58ac055c8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_FederalHomeLoanBankAdvancesBranchOfFHLBBankStateDomain_38af9327-4dd1-4b59-95b9-c7b58ac055c8_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_FederalHomeLoanBankAdvancesBranchOfFHLBBankStateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_FederalHomeLoanBankAdvancesBranchOfFHLBBankAxis_38af9327-4dd1-4b59-95b9-c7b58ac055c8" xlink:to="loc_srt_FederalHomeLoanBankAdvancesBranchOfFHLBBankStateDomain_38af9327-4dd1-4b59-95b9-c7b58ac055c8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_FederalHomeLoanBankAdvancesBranchOfFHLBBankStateDomain_09368fde-ee0f-4427-9448-1e204a851f46" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_FederalHomeLoanBankAdvancesBranchOfFHLBBankStateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_FederalHomeLoanBankAdvancesBranchOfFHLBBankAxis_38af9327-4dd1-4b59-95b9-c7b58ac055c8" xlink:to="loc_srt_FederalHomeLoanBankAdvancesBranchOfFHLBBankStateDomain_09368fde-ee0f-4427-9448-1e204a851f46" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_FederalHomeLoanBankOfDesMoinesMember_4dd5a11b-3af7-4715-b7e5-231b550462f9" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_FederalHomeLoanBankOfDesMoinesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_FederalHomeLoanBankAdvancesBranchOfFHLBBankStateDomain_09368fde-ee0f-4427-9448-1e204a851f46" xlink:to="loc_srt_FederalHomeLoanBankOfDesMoinesMember_4dd5a11b-3af7-4715-b7e5-231b550462f9" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/OtherBorrowingsandNotesPayableDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#OtherBorrowingsandNotesPayableDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/OtherBorrowingsandNotesPayableDetails" xlink:type="extended" id="i504bb309104d4fdb8a1bd28d2883d605_OtherBorrowingsandNotesPayableDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_e773833d-cbcd-4421-b109-9040359e2a3c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuritiesSoldUnderAgreementsToRepurchase_425d2d89-2407-4ebe-8eaf-65ab3e242b83" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SecuritiesSoldUnderAgreementsToRepurchase"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e773833d-cbcd-4421-b109-9040359e2a3c" xlink:to="loc_us-gaap_SecuritiesSoldUnderAgreementsToRepurchase_425d2d89-2407-4ebe-8eaf-65ab3e242b83" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ShortTermAndLongTermDebtAverageOutstandingAmount_4940aabc-452e-499c-8373-1b7f4b9a213e" xlink:href="efsc-20221231.xsd#efsc_ShortTermAndLongTermDebtAverageOutstandingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e773833d-cbcd-4421-b109-9040359e2a3c" xlink:to="loc_efsc_ShortTermAndLongTermDebtAverageOutstandingAmount_4940aabc-452e-499c-8373-1b7f4b9a213e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ShortTermAndLongTermDebtMaximumMonthEndOutstandingAmount_2f18016b-61f7-4df6-a505-84010afa05d9" xlink:href="efsc-20221231.xsd#efsc_ShortTermAndLongTermDebtMaximumMonthEndOutstandingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e773833d-cbcd-4421-b109-9040359e2a3c" xlink:to="loc_efsc_ShortTermAndLongTermDebtMaximumMonthEndOutstandingAmount_2f18016b-61f7-4df6-a505-84010afa05d9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateDuringPeriod_4ee13157-cd04-402a-bc92-fd3d54d35d0e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentInterestRateDuringPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e773833d-cbcd-4421-b109-9040359e2a3c" xlink:to="loc_us-gaap_DebtInstrumentInterestRateDuringPeriod_4ee13157-cd04-402a-bc92-fd3d54d35d0e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateEffectivePercentage_9bd83261-d53f-4bde-820b-d391fd78950a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentInterestRateEffectivePercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e773833d-cbcd-4421-b109-9040359e2a3c" xlink:to="loc_us-gaap_DebtInstrumentInterestRateEffectivePercentage_9bd83261-d53f-4bde-820b-d391fd78950a" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityRemainingBorrowingCapacity_3acdde58-ac9b-42ce-b1ee-f208068d1569" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LineOfCreditFacilityRemainingBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e773833d-cbcd-4421-b109-9040359e2a3c" xlink:to="loc_us-gaap_LineOfCreditFacilityRemainingBorrowingCapacity_3acdde58-ac9b-42ce-b1ee-f208068d1569" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PledgedFinancialInstrumentsNotSeparatelyReportedLoansReceivablePledgedForFederalHomeLoanBankDebt_09f212e6-90b3-4e1e-8904-7101d346ae3a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PledgedFinancialInstrumentsNotSeparatelyReportedLoansReceivablePledgedForFederalHomeLoanBankDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e773833d-cbcd-4421-b109-9040359e2a3c" xlink:to="loc_us-gaap_PledgedFinancialInstrumentsNotSeparatelyReportedLoansReceivablePledgedForFederalHomeLoanBankDebt_09f212e6-90b3-4e1e-8904-7101d346ae3a" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCredit_39518b4a-4485-4dc9-8880-e7c3ce5b0ec0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LineOfCredit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e773833d-cbcd-4421-b109-9040359e2a3c" xlink:to="loc_us-gaap_LineOfCredit_39518b4a-4485-4dc9-8880-e7c3ce5b0ec0" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherBorrowings_4caceb0c-fdd0-4457-a63f-744dd45ebe08" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherBorrowings"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e773833d-cbcd-4421-b109-9040359e2a3c" xlink:to="loc_us-gaap_OtherBorrowings_4caceb0c-fdd0-4457-a63f-744dd45ebe08" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTerm_5532aa04-8d1a-44d0-8a17-3e7cae7acda0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e773833d-cbcd-4421-b109-9040359e2a3c" xlink:to="loc_us-gaap_DebtInstrumentTerm_5532aa04-8d1a-44d0-8a17-3e7cae7acda0" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_52d3e4c8-f73c-422c-ac90-83f0784386ea" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentInterestRateStatedPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e773833d-cbcd-4421-b109-9040359e2a3c" xlink:to="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_52d3e4c8-f73c-422c-ac90-83f0784386ea" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_DebtInstrumentInterestTerm_7b4f5c5b-03a2-46f9-b9b4-b6f89d16fd3d" xlink:href="efsc-20221231.xsd#efsc_DebtInstrumentInterestTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e773833d-cbcd-4421-b109-9040359e2a3c" xlink:to="loc_efsc_DebtInstrumentInterestTerm_7b4f5c5b-03a2-46f9-b9b4-b6f89d16fd3d" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LinesOfCreditCurrent_49a0abef-1810-4d70-80f8-2506ac48df90" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LinesOfCreditCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e773833d-cbcd-4421-b109-9040359e2a3c" xlink:to="loc_us-gaap_LinesOfCreditCurrent_49a0abef-1810-4d70-80f8-2506ac48df90" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_4f05327d-330b-4329-bb9c-db59a0628d0f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentBasisSpreadOnVariableRate1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e773833d-cbcd-4421-b109-9040359e2a3c" xlink:to="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_4f05327d-330b-4329-bb9c-db59a0628d0f" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentFaceAmount_ffb6aeb5-f33e-4d07-b8e2-9f42f4dff01a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentFaceAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e773833d-cbcd-4421-b109-9040359e2a3c" xlink:to="loc_us-gaap_DebtInstrumentFaceAmount_ffb6aeb5-f33e-4d07-b8e2-9f42f4dff01a" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LoansPayable_8bd0fb54-0aa6-40d9-baf1-26ee40713e91" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LoansPayable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e773833d-cbcd-4421-b109-9040359e2a3c" xlink:to="loc_us-gaap_LoansPayable_8bd0fb54-0aa6-40d9-baf1-26ee40713e91" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_797a2443-ab72-4d92-a59c-f3521ae3815a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e773833d-cbcd-4421-b109-9040359e2a3c" xlink:to="loc_us-gaap_DebtInstrumentTable_797a2443-ab72-4d92-a59c-f3521ae3815a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_ff052a16-af93-4820-bc12-a4bea98306d6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_797a2443-ab72-4d92-a59c-f3521ae3815a" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_ff052a16-af93-4820-bc12-a4bea98306d6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_ff052a16-af93-4820-bc12-a4bea98306d6_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_ff052a16-af93-4820-bc12-a4bea98306d6" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_ff052a16-af93-4820-bc12-a4bea98306d6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_1c7b047b-84ba-45b5-885f-45be23f9fd5c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_ff052a16-af93-4820-bc12-a4bea98306d6" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_1c7b047b-84ba-45b5-885f-45be23f9fd5c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BorrowingsMember_db07922f-bef4-4c6d-867d-cf07d0d7b660" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_BorrowingsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_1c7b047b-84ba-45b5-885f-45be23f9fd5c" xlink:to="loc_us-gaap_BorrowingsMember_db07922f-bef4-4c6d-867d-cf07d0d7b660" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditMember_c54e965a-0ef4-4ca6-8ef3-47a4b093f96f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LineOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_1c7b047b-84ba-45b5-885f-45be23f9fd5c" xlink:to="loc_us-gaap_LineOfCreditMember_c54e965a-0ef4-4ca6-8ef3-47a4b093f96f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnsecuredDebtMember_970ca52e-c729-4fd1-9c99-6d6de5d910b2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_UnsecuredDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_1c7b047b-84ba-45b5-885f-45be23f9fd5c" xlink:to="loc_us-gaap_UnsecuredDebtMember_970ca52e-c729-4fd1-9c99-6d6de5d910b2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_c464d61d-b2db-407a-8a9a-2e59adb7ba03" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_797a2443-ab72-4d92-a59c-f3521ae3815a" xlink:to="loc_us-gaap_DebtInstrumentAxis_c464d61d-b2db-407a-8a9a-2e59adb7ba03" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_c464d61d-b2db-407a-8a9a-2e59adb7ba03_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_c464d61d-b2db-407a-8a9a-2e59adb7ba03" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_c464d61d-b2db-407a-8a9a-2e59adb7ba03_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_15dbe827-f32c-4ead-beb0-b490517c4d64" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_c464d61d-b2db-407a-8a9a-2e59adb7ba03" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_15dbe827-f32c-4ead-beb0-b490517c4d64" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_NewMarketTaxCreditInvestmentsMember_814833da-1fe9-481a-ae95-8fc5348e382b" xlink:href="efsc-20221231.xsd#efsc_NewMarketTaxCreditInvestmentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_15dbe827-f32c-4ead-beb0-b490517c4d64" xlink:to="loc_efsc_NewMarketTaxCreditInvestmentsMember_814833da-1fe9-481a-ae95-8fc5348e382b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_SeniorUnsecuredRevolvingCreditAgreementRevolvingAgreementMember_f2a15dbb-7ccc-4330-9537-ba756caa38ca" xlink:href="efsc-20221231.xsd#efsc_SeniorUnsecuredRevolvingCreditAgreementRevolvingAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_15dbe827-f32c-4ead-beb0-b490517c4d64" xlink:to="loc_efsc_SeniorUnsecuredRevolvingCreditAgreementRevolvingAgreementMember_f2a15dbb-7ccc-4330-9537-ba756caa38ca" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_UnsecuredTermLoanAgreementTermLoanMember_5e7e53b6-720c-4267-801f-d1ac530b600a" xlink:href="efsc-20221231.xsd#efsc_UnsecuredTermLoanAgreementTermLoanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_15dbe827-f32c-4ead-beb0-b490517c4d64" xlink:to="loc_efsc_UnsecuredTermLoanAgreementTermLoanMember_5e7e53b6-720c-4267-801f-d1ac530b600a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_ff95b542-1a67-4484-95dd-88adaf0f71b5" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_797a2443-ab72-4d92-a59c-f3521ae3815a" xlink:to="loc_srt_RangeAxis_ff95b542-1a67-4484-95dd-88adaf0f71b5" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_ff95b542-1a67-4484-95dd-88adaf0f71b5_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_ff95b542-1a67-4484-95dd-88adaf0f71b5" xlink:to="loc_srt_RangeMember_ff95b542-1a67-4484-95dd-88adaf0f71b5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_d6832d41-09c4-42de-aa42-21a4fc54be34" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_ff95b542-1a67-4484-95dd-88adaf0f71b5" xlink:to="loc_srt_RangeMember_d6832d41-09c4-42de-aa42-21a4fc54be34" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_f6fef871-a983-4520-aa56-211628b2dfc1" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_d6832d41-09c4-42de-aa42-21a4fc54be34" xlink:to="loc_srt_MinimumMember_f6fef871-a983-4520-aa56-211628b2dfc1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_1fd44a8c-41d3-4f69-9aa5-985f6846948c" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_d6832d41-09c4-42de-aa42-21a4fc54be34" xlink:to="loc_srt_MaximumMember_1fd44a8c-41d3-4f69-9aa5-985f6846948c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityAxis_673bf3c5-c3ee-46f1-b283-2e432aae5e2c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_797a2443-ab72-4d92-a59c-f3521ae3815a" xlink:to="loc_us-gaap_CreditFacilityAxis_673bf3c5-c3ee-46f1-b283-2e432aae5e2c" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_673bf3c5-c3ee-46f1-b283-2e432aae5e2c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CreditFacilityAxis_673bf3c5-c3ee-46f1-b283-2e432aae5e2c" xlink:to="loc_us-gaap_CreditFacilityDomain_673bf3c5-c3ee-46f1-b283-2e432aae5e2c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_5da22584-265b-4e66-9fd4-a2b9d8e43d7e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CreditFacilityAxis_673bf3c5-c3ee-46f1-b283-2e432aae5e2c" xlink:to="loc_us-gaap_CreditFacilityDomain_5da22584-265b-4e66-9fd4-a2b9d8e43d7e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevolvingCreditFacilityMember_c07d485e-588c-417b-abab-29f14a75ba45" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RevolvingCreditFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_5da22584-265b-4e66-9fd4-a2b9d8e43d7e" xlink:to="loc_us-gaap_RevolvingCreditFacilityMember_c07d485e-588c-417b-abab-29f14a75ba45" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateAxis_3e35813d-1109-4dcf-bec8-8f35f9d140d9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_VariableRateAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_797a2443-ab72-4d92-a59c-f3521ae3815a" xlink:to="loc_us-gaap_VariableRateAxis_3e35813d-1109-4dcf-bec8-8f35f9d140d9" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_3e35813d-1109-4dcf-bec8-8f35f9d140d9_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_VariableRateAxis_3e35813d-1109-4dcf-bec8-8f35f9d140d9" xlink:to="loc_us-gaap_VariableRateDomain_3e35813d-1109-4dcf-bec8-8f35f9d140d9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_6e6ec389-e2ca-4491-ae25-571a0bce7bfa" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_VariableRateAxis_3e35813d-1109-4dcf-bec8-8f35f9d140d9" xlink:to="loc_us-gaap_VariableRateDomain_6e6ec389-e2ca-4491-ae25-571a0bce7bfa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LondonInterbankOfferedRateLIBORMember_1d47a113-17b0-4b9e-895e-59aac601bb46" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LondonInterbankOfferedRateLIBORMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableRateDomain_6e6ec389-e2ca-4491-ae25-571a0bce7bfa" xlink:to="loc_us-gaap_LondonInterbankOfferedRateLIBORMember_1d47a113-17b0-4b9e-895e-59aac601bb46" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_SecuredOvernightFinancingRateSOFRMember_9324f3cf-94dd-4cc1-a8d7-b57db3fee1a4" xlink:href="efsc-20221231.xsd#efsc_SecuredOvernightFinancingRateSOFRMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableRateDomain_6e6ec389-e2ca-4491-ae25-571a0bce7bfa" xlink:to="loc_efsc_SecuredOvernightFinancingRateSOFRMember_9324f3cf-94dd-4cc1-a8d7-b57db3fee1a4" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/RegulatoryMattersDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#RegulatoryMattersDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/RegulatoryMattersDetails" xlink:type="extended" id="i2eb071ba72f64a9bac8e78056f265be8_RegulatoryMattersDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryAssetLineItems_178230a9-0eaf-41a9-9708-37a4d4be2e83" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RegulatoryAssetLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_TierOneCommonEquityCapitaltoRiskWeightedAssets_84e6032e-a8ea-4a51-8406-bfc4e1d70f31" xlink:href="efsc-20221231.xsd#efsc_TierOneCommonEquityCapitaltoRiskWeightedAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryAssetLineItems_178230a9-0eaf-41a9-9708-37a4d4be2e83" xlink:to="loc_efsc_TierOneCommonEquityCapitaltoRiskWeightedAssets_84e6032e-a8ea-4a51-8406-bfc4e1d70f31" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_TierOneCommonEquityCapitalRequiredtobeWellCapitalizedtoRiskWeightedAssets_b0d4c417-581a-4cda-929a-dd6d350c8125" xlink:href="efsc-20221231.xsd#efsc_TierOneCommonEquityCapitalRequiredtobeWellCapitalizedtoRiskWeightedAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryAssetLineItems_178230a9-0eaf-41a9-9708-37a4d4be2e83" xlink:to="loc_efsc_TierOneCommonEquityCapitalRequiredtobeWellCapitalizedtoRiskWeightedAssets_b0d4c417-581a-4cda-929a-dd6d350c8125" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_TierOneCommonEquityCapitalCCBMinimum_2f47723e-1fb8-4a02-a99e-7d3bc15aa2fd" xlink:href="efsc-20221231.xsd#efsc_TierOneCommonEquityCapitalCCBMinimum"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryAssetLineItems_178230a9-0eaf-41a9-9708-37a4d4be2e83" xlink:to="loc_efsc_TierOneCommonEquityCapitalCCBMinimum_2f47723e-1fb8-4a02-a99e-7d3bc15aa2fd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TierOneRiskBasedCapitalToRiskWeightedAssets_9dfd184e-2282-4f80-84f2-79e318289a88" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TierOneRiskBasedCapitalToRiskWeightedAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryAssetLineItems_178230a9-0eaf-41a9-9708-37a4d4be2e83" xlink:to="loc_us-gaap_TierOneRiskBasedCapitalToRiskWeightedAssets_9dfd184e-2282-4f80-84f2-79e318289a88" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TierOneRiskBasedCapitalRequiredToBeWellCapitalizedToRiskWeightedAssets_ebb1db51-d6cd-4078-bdbe-ab9f49320025" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TierOneRiskBasedCapitalRequiredToBeWellCapitalizedToRiskWeightedAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryAssetLineItems_178230a9-0eaf-41a9-9708-37a4d4be2e83" xlink:to="loc_us-gaap_TierOneRiskBasedCapitalRequiredToBeWellCapitalizedToRiskWeightedAssets_ebb1db51-d6cd-4078-bdbe-ab9f49320025" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_TierOneRiskBasedCapitalCCBMinimum_9228cc0b-ad07-4172-9a4a-5baf0068199c" xlink:href="efsc-20221231.xsd#efsc_TierOneRiskBasedCapitalCCBMinimum"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryAssetLineItems_178230a9-0eaf-41a9-9708-37a4d4be2e83" xlink:to="loc_efsc_TierOneRiskBasedCapitalCCBMinimum_9228cc0b-ad07-4172-9a4a-5baf0068199c" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CapitalToRiskWeightedAssets_ce178e3a-ae7e-4e61-a424-ade73f2797d9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CapitalToRiskWeightedAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryAssetLineItems_178230a9-0eaf-41a9-9708-37a4d4be2e83" xlink:to="loc_us-gaap_CapitalToRiskWeightedAssets_ce178e3a-ae7e-4e61-a424-ade73f2797d9" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CapitalRequiredToBeWellCapitalizedToRiskWeightedAssets_3831fd80-f51c-4c1a-81bb-1aa39e1a462a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CapitalRequiredToBeWellCapitalizedToRiskWeightedAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryAssetLineItems_178230a9-0eaf-41a9-9708-37a4d4be2e83" xlink:to="loc_us-gaap_CapitalRequiredToBeWellCapitalizedToRiskWeightedAssets_3831fd80-f51c-4c1a-81bb-1aa39e1a462a" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CapitaltoRiskWeightedAssetsCCBMinimum_3438b52f-1d29-4fe5-8cba-a90cf0df8c8d" xlink:href="efsc-20221231.xsd#efsc_CapitaltoRiskWeightedAssetsCCBMinimum"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryAssetLineItems_178230a9-0eaf-41a9-9708-37a4d4be2e83" xlink:to="loc_efsc_CapitaltoRiskWeightedAssetsCCBMinimum_3438b52f-1d29-4fe5-8cba-a90cf0df8c8d" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TierOneLeverageCapitalToAverageAssets_01af6f85-c2b9-4fe9-9c44-54e693ff8faa" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TierOneLeverageCapitalToAverageAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryAssetLineItems_178230a9-0eaf-41a9-9708-37a4d4be2e83" xlink:to="loc_us-gaap_TierOneLeverageCapitalToAverageAssets_01af6f85-c2b9-4fe9-9c44-54e693ff8faa" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TierOneLeverageCapitalRequiredToBeWellCapitalizedToAverageAssets_17e6c02d-c671-44a1-8f83-c7279681dd2b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TierOneLeverageCapitalRequiredToBeWellCapitalizedToAverageAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryAssetLineItems_178230a9-0eaf-41a9-9708-37a4d4be2e83" xlink:to="loc_us-gaap_TierOneLeverageCapitalRequiredToBeWellCapitalizedToAverageAssets_17e6c02d-c671-44a1-8f83-c7279681dd2b" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_TierOneLeverageCapitaltoAverageAssetsCCBMinimum_bae9a911-a0bc-4898-8767-bbce19c39945" xlink:href="efsc-20221231.xsd#efsc_TierOneLeverageCapitaltoAverageAssetsCCBMinimum"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RegulatoryAssetLineItems_178230a9-0eaf-41a9-9708-37a4d4be2e83" xlink:to="loc_efsc_TierOneLeverageCapitaltoAverageAssetsCCBMinimum_bae9a911-a0bc-4898-8767-bbce19c39945" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRegulatoryAssetsTable_5c75fde8-3e24-46df-9ac5-12ea4726ef02" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfRegulatoryAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RegulatoryAssetLineItems_178230a9-0eaf-41a9-9708-37a4d4be2e83" xlink:to="loc_us-gaap_ScheduleOfRegulatoryAssetsTable_5c75fde8-3e24-46df-9ac5-12ea4726ef02" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_83936721-42c3-423d-ae5e-ea7bdf5723a1" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRegulatoryAssetsTable_5c75fde8-3e24-46df-9ac5-12ea4726ef02" xlink:to="loc_dei_LegalEntityAxis_83936721-42c3-423d-ae5e-ea7bdf5723a1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_83936721-42c3-423d-ae5e-ea7bdf5723a1_default" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_83936721-42c3-423d-ae5e-ea7bdf5723a1" xlink:to="loc_dei_EntityDomain_83936721-42c3-423d-ae5e-ea7bdf5723a1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_756cdaea-b039-456b-9518-2669f06eb507" xlink:href="https://xbrl.sec.gov/dei/2021q4/dei-2021q4.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_83936721-42c3-423d-ae5e-ea7bdf5723a1" xlink:to="loc_dei_EntityDomain_756cdaea-b039-456b-9518-2669f06eb507" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_EnterpriseFinancialServicesCorpMember_3737a672-22bb-48c8-8d2b-8983b4c5ef40" xlink:href="efsc-20221231.xsd#efsc_EnterpriseFinancialServicesCorpMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_756cdaea-b039-456b-9518-2669f06eb507" xlink:to="loc_efsc_EnterpriseFinancialServicesCorpMember_3737a672-22bb-48c8-8d2b-8983b4c5ef40" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_EnterpriseBankAndTrustMember_42b51e01-b533-426b-a5b6-f71b01eb0367" xlink:href="efsc-20221231.xsd#efsc_EnterpriseBankAndTrustMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_756cdaea-b039-456b-9518-2669f06eb507" xlink:to="loc_efsc_EnterpriseBankAndTrustMember_42b51e01-b533-426b-a5b6-f71b01eb0367" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryAssetAxis_998eeb36-eadd-44fa-9519-a8aa0b9c177d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RegulatoryAssetAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRegulatoryAssetsTable_5c75fde8-3e24-46df-9ac5-12ea4726ef02" xlink:to="loc_us-gaap_RegulatoryAssetAxis_998eeb36-eadd-44fa-9519-a8aa0b9c177d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryAssetDomain_998eeb36-eadd-44fa-9519-a8aa0b9c177d_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RegulatoryAssetDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RegulatoryAssetAxis_998eeb36-eadd-44fa-9519-a8aa0b9c177d" xlink:to="loc_us-gaap_RegulatoryAssetDomain_998eeb36-eadd-44fa-9519-a8aa0b9c177d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RegulatoryAssetDomain_17fc1f89-0803-465b-8c7e-432c9b387765" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RegulatoryAssetDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RegulatoryAssetAxis_998eeb36-eadd-44fa-9519-a8aa0b9c177d" xlink:to="loc_us-gaap_RegulatoryAssetDomain_17fc1f89-0803-465b-8c7e-432c9b387765" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/CompensationPlansNarrativeDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#CompensationPlansNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/CompensationPlansNarrativeDetails" xlink:type="extended" id="i375998cc552c4587985ae24b6e8ada40_CompensationPlansNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardPurchasePriceOfCommonStockPercent_ef3e255b-ffd7-44c0-869e-a5530a49982d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardPurchasePriceOfCommonStockPercent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardPurchasePriceOfCommonStockPercent_ef3e255b-ffd7-44c0-869e-a5530a49982d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockRepurchaseProgramNumberOfSharesAuthorizedToBeRepurchased_f7bcd31c-2068-42e7-ae65-ce9f3151d8d5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockRepurchaseProgramNumberOfSharesAuthorizedToBeRepurchased"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_us-gaap_StockRepurchaseProgramNumberOfSharesAuthorizedToBeRepurchased_f7bcd31c-2068-42e7-ae65-ce9f3151d8d5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrant_f0170dcc-0e39-45b1-849a-fb4818102b4c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrant"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrant_f0170dcc-0e39-45b1-849a-fb4818102b4c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllocatedShareBasedCompensationExpense_b8ddac4b-9e1c-441a-b88e-ab456ac1677c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AllocatedShareBasedCompensationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_us-gaap_AllocatedShareBasedCompensationExpense_b8ddac4b-9e1c-441a-b88e-ab456ac1677c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationTaxBenefitFromCompensationExpense_f269e7a6-a512-4148-b807-e07a47799d38" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationTaxBenefitFromCompensationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationTaxBenefitFromCompensationExpense_f269e7a6-a512-4148-b807-e07a47799d38" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1_ea3dd261-1a8e-446f-9e7c-2bd1fbe40996" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1_ea3dd261-1a8e-446f-9e7c-2bd1fbe40996" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardExpirationPeriod_3859079c-de89-4c7f-89ec-9609a822952f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardExpirationPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardExpirationPeriod_3859079c-de89-4c7f-89ec-9609a822952f" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_82ad4ea0-5e43-4742-a54b-6713eb70fe69" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_82ad4ea0-5e43-4742-a54b-6713eb70fe69" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized_981df730-e3ac-481a-be48-dabed6044b86" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized_981df730-e3ac-481a-be48-dabed6044b86" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardSharesIssuedInPeriod_04b02877-c4a6-4bd7-8b55-4dbe563bc97c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardSharesIssuedInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardSharesIssuedInPeriod_04b02877-c4a6-4bd7-8b55-4dbe563bc97c" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod_b498b8dc-1d50-40ba-a524-23a0afdf4c77" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod_b498b8dc-1d50-40ba-a524-23a0afdf4c77" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_DefinedContributionPlanEmployeeMinimumAgeToParticipate_fba8f5ec-19d9-48b8-9485-33c4dc070f6e" xlink:href="efsc-20221231.xsd#efsc_DefinedContributionPlanEmployeeMinimumAgeToParticipate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_efsc_DefinedContributionPlanEmployeeMinimumAgeToParticipate_fba8f5ec-19d9-48b8-9485-33c4dc070f6e" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedContributionPlanCostRecognized_f627af3a-71a0-4000-a683-05307f92f683" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DefinedContributionPlanCostRecognized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_us-gaap_DefinedContributionPlanCostRecognized_f627af3a-71a0-4000-a683-05307f92f683" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesAuthorized_efed8373-9bbb-4fe8-a08b-97061164ed2f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PreferredStockSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_us-gaap_PreferredStockSharesAuthorized_efed8373-9bbb-4fe8-a08b-97061164ed2f" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockParOrStatedValuePerShare_899cdc51-b807-4ff4-a5be-7475c027f5e2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PreferredStockParOrStatedValuePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_us-gaap_PreferredStockParOrStatedValuePerShare_899cdc51-b807-4ff4-a5be-7475c027f5e2" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesOutstanding_f310c4e1-2d02-47bd-9ded-41f1c9f6f3eb" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PreferredStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_us-gaap_PreferredStockSharesOutstanding_f310c4e1-2d02-47bd-9ded-41f1c9f6f3eb" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_a4560bef-a9c0-4864-8152-f308539d4023" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_a4560bef-a9c0-4864-8152-f308539d4023" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardMaximumEmployeeSubscriptionRate_f117e547-81da-49b2-a5cb-4c048f2ed470" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardMaximumEmployeeSubscriptionRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardMaximumEmployeeSubscriptionRate_f117e547-81da-49b2-a5cb-4c048f2ed470" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_SharebasedCompensationArrangementbySharebasedPaymentAwardMaximumEmployeeSubscriptionRateBonus_1f65a62c-4f95-4d27-9763-3e03fb668003" xlink:href="efsc-20221231.xsd#efsc_SharebasedCompensationArrangementbySharebasedPaymentAwardMaximumEmployeeSubscriptionRateBonus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_efsc_SharebasedCompensationArrangementbySharebasedPaymentAwardMaximumEmployeeSubscriptionRateBonus_1f65a62c-4f95-4d27-9763-3e03fb668003" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredCompensationLiabilityCurrentAndNoncurrent_503e1cb6-f624-4b83-bb0f-8d4b719d7040" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DeferredCompensationLiabilityCurrentAndNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_us-gaap_DeferredCompensationLiabilityCurrentAndNoncurrent_503e1cb6-f624-4b83-bb0f-8d4b719d7040" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrantExcludingDeferredShares_560978a5-6aa0-4177-afe8-c5090d944bc4" xlink:href="efsc-20221231.xsd#efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrantExcludingDeferredShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrantExcludingDeferredShares_560978a5-6aa0-4177-afe8-c5090d944bc4" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ShareBasedCompensationArrangementBySharBasedPaymentAwardNumberOfSharesAvailableForGrantDeferredShares_866662a7-4f5c-4af1-8009-f69fc6328886" xlink:href="efsc-20221231.xsd#efsc_ShareBasedCompensationArrangementBySharBasedPaymentAwardNumberOfSharesAvailableForGrantDeferredShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_efsc_ShareBasedCompensationArrangementBySharBasedPaymentAwardNumberOfSharesAvailableForGrantDeferredShares_866662a7-4f5c-4af1-8009-f69fc6328886" xlink:type="arc" order="21"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOfPreferredStockAndPreferenceStock_7848c13c-0b52-4c81-bce3-aa67b04942ff" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ProceedsFromIssuanceOfPreferredStockAndPreferenceStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_us-gaap_ProceedsFromIssuanceOfPreferredStockAndPreferenceStock_7848c13c-0b52-4c81-bce3-aa67b04942ff" xlink:type="arc" order="22"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_DepositarySharesIssuedAndSold_56f60a84-5e8a-4aff-96cf-da533973b8fd" xlink:href="efsc-20221231.xsd#efsc_DepositarySharesIssuedAndSold"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_efsc_DepositarySharesIssuedAndSold_56f60a84-5e8a-4aff-96cf-da533973b8fd" xlink:type="arc" order="23"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsVestedInPeriodFairValue1_75687741-39d5-485d-834f-18cce7a9d6ce" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsVestedInPeriodFairValue1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsVestedInPeriodFairValue1_75687741-39d5-485d-834f-18cce7a9d6ce" xlink:type="arc" order="24"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingIntrinsicValue_478639a1-5b9f-4dec-9464-dea5cfeb5ae4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingIntrinsicValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingIntrinsicValue_478639a1-5b9f-4dec-9464-dea5cfeb5ae4" xlink:type="arc" order="25"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardSharesAvailableUnderEmployeeStockPurchasePlan_01f0cd43-e264-4de1-86de-e2eece3eb7be" xlink:href="efsc-20221231.xsd#efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardSharesAvailableUnderEmployeeStockPurchasePlan"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardSharesAvailableUnderEmployeeStockPurchasePlan_01f0cd43-e264-4de1-86de-e2eece3eb7be" xlink:type="arc" order="26"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardExercisesInPeriod_c41ca865-e225-4883-ba9d-7faa4001b2fc" xlink:href="efsc-20221231.xsd#efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardExercisesInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardExercisesInPeriod_c41ca865-e225-4883-ba9d-7faa4001b2fc" xlink:type="arc" order="27"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_8a88909c-ec72-48c4-be19-2ce46b70869b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_71a7f6fa-d0f8-42a1-90d3-8778596913f8" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_8a88909c-ec72-48c4-be19-2ce46b70869b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameAxis_446d3620-7d2b-4ad5-8c26-1c5d017de6d2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PlanNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_8a88909c-ec72-48c4-be19-2ce46b70869b" xlink:to="loc_us-gaap_PlanNameAxis_446d3620-7d2b-4ad5-8c26-1c5d017de6d2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_446d3620-7d2b-4ad5-8c26-1c5d017de6d2_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PlanNameAxis_446d3620-7d2b-4ad5-8c26-1c5d017de6d2" xlink:to="loc_us-gaap_PlanNameDomain_446d3620-7d2b-4ad5-8c26-1c5d017de6d2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_f4ae31e4-b01c-4042-be2f-c05ee31355a3" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PlanNameAxis_446d3620-7d2b-4ad5-8c26-1c5d017de6d2" xlink:to="loc_us-gaap_PlanNameDomain_f4ae31e4-b01c-4042-be2f-c05ee31355a3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_StockPlanForNonManagementDirectorsMember_9d8e22a3-5c31-449f-ab68-5acdd0c1866e" xlink:href="efsc-20221231.xsd#efsc_StockPlanForNonManagementDirectorsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_f4ae31e4-b01c-4042-be2f-c05ee31355a3" xlink:to="loc_efsc_StockPlanForNonManagementDirectorsMember_9d8e22a3-5c31-449f-ab68-5acdd0c1866e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_DeferredCompensationPlanMember_e4a7bc62-7723-41be-9f9a-1eeedf24dbd4" xlink:href="efsc-20221231.xsd#efsc_DeferredCompensationPlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_f4ae31e4-b01c-4042-be2f-c05ee31355a3" xlink:to="loc_efsc_DeferredCompensationPlanMember_e4a7bc62-7723-41be-9f9a-1eeedf24dbd4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_ff3248a0-959c-4b60-a0be-039a9c468821" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_8a88909c-ec72-48c4-be19-2ce46b70869b" xlink:to="loc_srt_RangeAxis_ff3248a0-959c-4b60-a0be-039a9c468821" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_ff3248a0-959c-4b60-a0be-039a9c468821_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_ff3248a0-959c-4b60-a0be-039a9c468821" xlink:to="loc_srt_RangeMember_ff3248a0-959c-4b60-a0be-039a9c468821_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_85d4e53a-4462-49bb-8b23-294224c2085c" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_ff3248a0-959c-4b60-a0be-039a9c468821" xlink:to="loc_srt_RangeMember_85d4e53a-4462-49bb-8b23-294224c2085c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_3d290ded-3bc3-453d-ad8f-37af21bec321" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_85d4e53a-4462-49bb-8b23-294224c2085c" xlink:to="loc_srt_MinimumMember_3d290ded-3bc3-453d-ad8f-37af21bec321" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_c16ed0a6-8225-4529-8991-e03be6a81a36" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_85d4e53a-4462-49bb-8b23-294224c2085c" xlink:to="loc_srt_MaximumMember_c16ed0a6-8225-4529-8991-e03be6a81a36" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_99fe0b6d-3458-4499-98df-e6caee315e65" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_8a88909c-ec72-48c4-be19-2ce46b70869b" xlink:to="loc_us-gaap_AwardTypeAxis_99fe0b6d-3458-4499-98df-e6caee315e65" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_99fe0b6d-3458-4499-98df-e6caee315e65_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_99fe0b6d-3458-4499-98df-e6caee315e65" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_99fe0b6d-3458-4499-98df-e6caee315e65_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_d95ccc8b-751e-47d9-9bf2-b751d43b6d4a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_99fe0b6d-3458-4499-98df-e6caee315e65" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_d95ccc8b-751e-47d9-9bf2-b751d43b6d4a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockOptionMember_a405eb5b-f677-4076-8820-24bd507af442" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EmployeeStockOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_d95ccc8b-751e-47d9-9bf2-b751d43b6d4a" xlink:to="loc_us-gaap_EmployeeStockOptionMember_a405eb5b-f677-4076-8820-24bd507af442" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_11a147f4-a26a-4f9e-bb19-e3bde32889b6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_d95ccc8b-751e-47d9-9bf2-b751d43b6d4a" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_11a147f4-a26a-4f9e-bb19-e3bde32889b6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PerformanceSharesMember_5ea91f7c-62a8-429a-a909-eac809196366" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PerformanceSharesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_d95ccc8b-751e-47d9-9bf2-b751d43b6d4a" xlink:to="loc_us-gaap_PerformanceSharesMember_5ea91f7c-62a8-429a-a909-eac809196366" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockMember_30546031-8e87-4b59-aa9e-ab2da5a5fad1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EmployeeStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_d95ccc8b-751e-47d9-9bf2-b751d43b6d4a" xlink:to="loc_us-gaap_EmployeeStockMember_30546031-8e87-4b59-aa9e-ab2da5a5fad1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_StockOptionsAndStockSettledStockAppreciationRightsMember_d9ba2409-aacd-4fe7-8f63-0b6797889feb" xlink:href="efsc-20221231.xsd#efsc_StockOptionsAndStockSettledStockAppreciationRightsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_d95ccc8b-751e-47d9-9bf2-b751d43b6d4a" xlink:to="loc_efsc_StockOptionsAndStockSettledStockAppreciationRightsMember_d9ba2409-aacd-4fe7-8f63-0b6797889feb" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/CompensationPlansCommonStockDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#CompensationPlansCommonStockDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/CompensationPlansCommonStockDetails" xlink:type="extended" id="i049d94854b154f3c849203e1c212c2a6_CompensationPlansCommonStockDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_627be5e6-c652-4cca-a043-e2f05503c59a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_00aa74e6-1c34-4009-b699-7767e558167c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_627be5e6-c652-4cca-a043-e2f05503c59a" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_00aa74e6-1c34-4009-b699-7767e558167c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardOutstandingNumber_7ee45694-f55f-4ba0-90f8-84ae777e1ba8" xlink:href="efsc-20221231.xsd#efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardOutstandingNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_627be5e6-c652-4cca-a043-e2f05503c59a" xlink:to="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardOutstandingNumber_7ee45694-f55f-4ba0-90f8-84ae777e1ba8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrant_4de8897e-fe3b-49ac-be4a-128b4e3443f8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrant"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_627be5e6-c652-4cca-a043-e2f05503c59a" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrant_4de8897e-fe3b-49ac-be4a-128b4e3443f8" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockCapitalSharesReservedForFutureIssuance_bb5ded60-ad1e-4480-abf6-867a3ce0832d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommonStockCapitalSharesReservedForFutureIssuance"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_627be5e6-c652-4cca-a043-e2f05503c59a" xlink:to="loc_us-gaap_CommonStockCapitalSharesReservedForFutureIssuance_bb5ded60-ad1e-4480-abf6-867a3ce0832d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_7bb51f2d-be9b-4696-a5e1-28fb60604000" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_627be5e6-c652-4cca-a043-e2f05503c59a" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_7bb51f2d-be9b-4696-a5e1-28fb60604000" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_f4c6cd57-0d83-490b-89ce-7bcdcb003bb4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_7bb51f2d-be9b-4696-a5e1-28fb60604000" xlink:to="loc_us-gaap_AwardTypeAxis_f4c6cd57-0d83-490b-89ce-7bcdcb003bb4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_f4c6cd57-0d83-490b-89ce-7bcdcb003bb4_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_f4c6cd57-0d83-490b-89ce-7bcdcb003bb4" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_f4c6cd57-0d83-490b-89ce-7bcdcb003bb4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_c12e64a9-af6d-4af0-84c6-39831f80e5c6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_f4c6cd57-0d83-490b-89ce-7bcdcb003bb4" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_c12e64a9-af6d-4af0-84c6-39831f80e5c6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PerformanceSharesMember_74d8e7fc-b0c2-4139-9bf2-aa5dad1d5bc9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PerformanceSharesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_c12e64a9-af6d-4af0-84c6-39831f80e5c6" xlink:to="loc_us-gaap_PerformanceSharesMember_74d8e7fc-b0c2-4139-9bf2-aa5dad1d5bc9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_5917cba7-9d43-45cd-84c4-67860ffc0682" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_c12e64a9-af6d-4af0-84c6-39831f80e5c6" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_5917cba7-9d43-45cd-84c4-67860ffc0682" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_StockOptionsAndStockSettledStockAppreciationRightsMember_3854f689-dbff-4fbb-a984-5eeb22065415" xlink:href="efsc-20221231.xsd#efsc_StockOptionsAndStockSettledStockAppreciationRightsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_c12e64a9-af6d-4af0-84c6-39831f80e5c6" xlink:to="loc_efsc_StockOptionsAndStockSettledStockAppreciationRightsMember_3854f689-dbff-4fbb-a984-5eeb22065415" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameAxis_ba95a777-36fd-471a-9a3b-0720c1fa7055" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PlanNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_7bb51f2d-be9b-4696-a5e1-28fb60604000" xlink:to="loc_us-gaap_PlanNameAxis_ba95a777-36fd-471a-9a3b-0720c1fa7055" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_ba95a777-36fd-471a-9a3b-0720c1fa7055_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PlanNameAxis_ba95a777-36fd-471a-9a3b-0720c1fa7055" xlink:to="loc_us-gaap_PlanNameDomain_ba95a777-36fd-471a-9a3b-0720c1fa7055_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_c5454c60-0bf6-4a0d-966c-1e9e019653d3" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PlanNameAxis_ba95a777-36fd-471a-9a3b-0720c1fa7055" xlink:to="loc_us-gaap_PlanNameDomain_c5454c60-0bf6-4a0d-966c-1e9e019653d3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_A2018StockIncentivePlanMember_49ee246f-470a-403f-b740-c9ef4620c324" xlink:href="efsc-20221231.xsd#efsc_A2018StockIncentivePlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_c5454c60-0bf6-4a0d-966c-1e9e019653d3" xlink:to="loc_efsc_A2018StockIncentivePlanMember_49ee246f-470a-403f-b740-c9ef4620c324" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_StockPlanForNonManagementDirectorsMember_ddc4ae25-ff4c-4da5-add3-c0b1c1eaa20c" xlink:href="efsc-20221231.xsd#efsc_StockPlanForNonManagementDirectorsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_c5454c60-0bf6-4a0d-966c-1e9e019653d3" xlink:to="loc_efsc_StockPlanForNonManagementDirectorsMember_ddc4ae25-ff4c-4da5-add3-c0b1c1eaa20c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_EmployeeStockPurchasePlanMember_27596f67-ad4a-4f7f-a091-ce7794b8ccb9" xlink:href="efsc-20221231.xsd#efsc_EmployeeStockPurchasePlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_c5454c60-0bf6-4a0d-966c-1e9e019653d3" xlink:to="loc_efsc_EmployeeStockPurchasePlanMember_27596f67-ad4a-4f7f-a091-ce7794b8ccb9" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/CompensationPlansChangesinOCIDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#CompensationPlansChangesinOCIDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/CompensationPlansChangesinOCIDetails" xlink:type="extended" id="i4f0ab4e3646146758f26250ce3a60f08_CompensationPlansChangesinOCIDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_77ace544-c181-4232-86a0-7b165c304ecd" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_b8ad84eb-f9fa-42fd-86b6-6710aee93f51" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AOCIAttributableToParentNetOfTaxRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_77ace544-c181-4232-86a0-7b165c304ecd" xlink:to="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_b8ad84eb-f9fa-42fd-86b6-6710aee93f51" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_cc7a2039-76f2-4148-9bd6-368663edb458" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_b8ad84eb-f9fa-42fd-86b6-6710aee93f51" xlink:to="loc_us-gaap_StockholdersEquity_cc7a2039-76f2-4148-9bd6-368663edb458" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_371f716d-c8ed-4fd4-bfb6-bda0eb629abe" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_b8ad84eb-f9fa-42fd-86b6-6710aee93f51" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_371f716d-c8ed-4fd4-bfb6-bda0eb629abe" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesHeldToMaturityTransferDerivativeHedgeGainLoss_8277975c-9ddc-4ac5-9529-b3565f763a40" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DebtSecuritiesHeldToMaturityTransferDerivativeHedgeGainLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_b8ad84eb-f9fa-42fd-86b6-6710aee93f51" xlink:to="loc_us-gaap_DebtSecuritiesHeldToMaturityTransferDerivativeHedgeGainLoss_8277975c-9ddc-4ac5-9529-b3565f763a40" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_5b0e9727-6376-4db3-981e-13a4c805d3a1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_8b055b3c-741f-456d-8c33-8921d3f958ff" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_77ace544-c181-4232-86a0-7b165c304ecd" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_8b055b3c-741f-456d-8c33-8921d3f958ff" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_2fe09ab1-757a-4a3c-b294-7763a949d0be" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_8b055b3c-741f-456d-8c33-8921d3f958ff" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_2fe09ab1-757a-4a3c-b294-7763a949d0be" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_2fe09ab1-757a-4a3c-b294-7763a949d0be_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_2fe09ab1-757a-4a3c-b294-7763a949d0be" xlink:to="loc_us-gaap_EquityComponentDomain_2fe09ab1-757a-4a3c-b294-7763a949d0be_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_f1757dcc-9096-4005-93f6-62d38ee599a2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_2fe09ab1-757a-4a3c-b294-7763a949d0be" xlink:to="loc_us-gaap_EquityComponentDomain_f1757dcc-9096-4005-93f6-62d38ee599a2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_413a23ea-1175-4694-a053-0e301bda997d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_f1757dcc-9096-4005-93f6-62d38ee599a2" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_413a23ea-1175-4694-a053-0e301bda997d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedNetUnrealizedInvestmentGainLossMember_28675eaf-f4da-402c-a804-44e6e0d23c22" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AccumulatedNetUnrealizedInvestmentGainLossMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_f1757dcc-9096-4005-93f6-62d38ee599a2" xlink:to="loc_us-gaap_AccumulatedNetUnrealizedInvestmentGainLossMember_28675eaf-f4da-402c-a804-44e6e0d23c22" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedGainLossNetCashFlowHedgeParentMember_f9363920-728b-43ef-8292-add49a7d0ca6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AccumulatedGainLossNetCashFlowHedgeParentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_f1757dcc-9096-4005-93f6-62d38ee599a2" xlink:to="loc_us-gaap_AccumulatedGainLossNetCashFlowHedgeParentMember_f9363920-728b-43ef-8292-add49a7d0ca6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_AOCIAccumulatedGainLossHeldToMaturitySecuritiesParentMember_9cda9319-226c-41b8-a5b1-c8b84077c335" xlink:href="efsc-20221231.xsd#efsc_AOCIAccumulatedGainLossHeldToMaturitySecuritiesParentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_f1757dcc-9096-4005-93f6-62d38ee599a2" xlink:to="loc_efsc_AOCIAccumulatedGainLossHeldToMaturitySecuritiesParentMember_9cda9319-226c-41b8-a5b1-c8b84077c335" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/CompensationPlansReclassificationsOutofAOCIDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#CompensationPlansReclassificationsOutofAOCIDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/CompensationPlansReclassificationsOutofAOCIDetails" xlink:type="extended" id="i4a1ac4dac3d14a22a061d030eb860d6a_CompensationPlansReclassificationsOutofAOCIDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReclassificationAdjustmentOutOfAccumulatedOtherComprehensiveIncomeOnDerivativesLineItems_94c85a0f-eb8c-4a4d-bae7-cb77f9a3cddf" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ReclassificationAdjustmentOutOfAccumulatedOtherComprehensiveIncomeOnDerivativesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NoninterestIncome_96336e84-5700-4668-9e6f-90c097bfb8a1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NoninterestIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReclassificationAdjustmentOutOfAccumulatedOtherComprehensiveIncomeOnDerivativesLineItems_94c85a0f-eb8c-4a4d-bae7-cb77f9a3cddf" xlink:to="loc_us-gaap_NoninterestIncome_96336e84-5700-4668-9e6f-90c097bfb8a1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestIncomeExpenseNet_b56268ef-a078-409d-b68c-a4d759b7bade" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestIncomeExpenseNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReclassificationAdjustmentOutOfAccumulatedOtherComprehensiveIncomeOnDerivativesLineItems_94c85a0f-eb8c-4a4d-bae7-cb77f9a3cddf" xlink:to="loc_us-gaap_InterestIncomeExpenseNet_b56268ef-a078-409d-b68c-a4d759b7bade" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesMinorityInterestAndIncomeLossFromEquityMethodInvestments_1f64a430-abe8-4bf0-b316-9de927a99d41" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesMinorityInterestAndIncomeLossFromEquityMethodInvestments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReclassificationAdjustmentOutOfAccumulatedOtherComprehensiveIncomeOnDerivativesLineItems_94c85a0f-eb8c-4a4d-bae7-cb77f9a3cddf" xlink:to="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesMinorityInterestAndIncomeLossFromEquityMethodInvestments_1f64a430-abe8-4bf0-b316-9de927a99d41" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_e23ecde0-5971-4f31-888b-ad02eb570f20" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeTaxExpenseBenefit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReclassificationAdjustmentOutOfAccumulatedOtherComprehensiveIncomeOnDerivativesLineItems_94c85a0f-eb8c-4a4d-bae7-cb77f9a3cddf" xlink:to="loc_us-gaap_IncomeTaxExpenseBenefit_e23ecde0-5971-4f31-888b-ad02eb570f20" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_c9275075-80f5-48e9-bb57-c00b2ca8b805" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReclassificationAdjustmentOutOfAccumulatedOtherComprehensiveIncomeOnDerivativesLineItems_94c85a0f-eb8c-4a4d-bae7-cb77f9a3cddf" xlink:to="loc_us-gaap_NetIncomeLoss_c9275075-80f5-48e9-bb57-c00b2ca8b805" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReclassificationOutOfAccumulatedOtherComprehensiveIncomeTable_474a70c8-3e8b-48c1-8af2-0769ab84c3b6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ReclassificationOutOfAccumulatedOtherComprehensiveIncomeTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ReclassificationAdjustmentOutOfAccumulatedOtherComprehensiveIncomeOnDerivativesLineItems_94c85a0f-eb8c-4a4d-bae7-cb77f9a3cddf" xlink:to="loc_us-gaap_ReclassificationOutOfAccumulatedOtherComprehensiveIncomeTable_474a70c8-3e8b-48c1-8af2-0769ab84c3b6" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_3a1d93a8-c20d-4376-b6cd-0c63fe1fdb7f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ReclassificationOutOfAccumulatedOtherComprehensiveIncomeTable_474a70c8-3e8b-48c1-8af2-0769ab84c3b6" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_3a1d93a8-c20d-4376-b6cd-0c63fe1fdb7f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_3a1d93a8-c20d-4376-b6cd-0c63fe1fdb7f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_3a1d93a8-c20d-4376-b6cd-0c63fe1fdb7f" xlink:to="loc_us-gaap_EquityComponentDomain_3a1d93a8-c20d-4376-b6cd-0c63fe1fdb7f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_00ca5d9c-b562-4db3-b722-6ca3220e5a05" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_3a1d93a8-c20d-4376-b6cd-0c63fe1fdb7f" xlink:to="loc_us-gaap_EquityComponentDomain_00ca5d9c-b562-4db3-b722-6ca3220e5a05" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedNetUnrealizedInvestmentGainLossMember_4667e7fd-0a3d-4e46-bf1c-387f8e2d0d2f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AccumulatedNetUnrealizedInvestmentGainLossMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_00ca5d9c-b562-4db3-b722-6ca3220e5a05" xlink:to="loc_us-gaap_AccumulatedNetUnrealizedInvestmentGainLossMember_4667e7fd-0a3d-4e46-bf1c-387f8e2d0d2f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedGainLossNetCashFlowHedgeParentMember_e7fafa44-3546-415c-bf6a-20c793989934" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AccumulatedGainLossNetCashFlowHedgeParentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_00ca5d9c-b562-4db3-b722-6ca3220e5a05" xlink:to="loc_us-gaap_AccumulatedGainLossNetCashFlowHedgeParentMember_e7fafa44-3546-415c-bf6a-20c793989934" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/CompensationPlansScheduleofVariousInformationDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#CompensationPlansScheduleofVariousInformationDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/CompensationPlansScheduleofVariousInformationDetails" xlink:type="extended" id="i9cebf37713864323bdb0457746764c3c_CompensationPlansScheduleofVariousInformationDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_4a59007e-9957-43df-a396-93a533d780fa" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllocatedShareBasedCompensationExpense_8da471df-dd59-4bb1-a052-712232ec13be" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AllocatedShareBasedCompensationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_4a59007e-9957-43df-a396-93a533d780fa" xlink:to="loc_us-gaap_AllocatedShareBasedCompensationExpense_8da471df-dd59-4bb1-a052-712232ec13be" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodTotalFairValue_58a4d0fe-1adc-4d3d-b914-a36cbcbb48b0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodTotalFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_4a59007e-9957-43df-a396-93a533d780fa" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodTotalFairValue_58a4d0fe-1adc-4d3d-b914-a36cbcbb48b0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_3856addc-2524-41c0-af6a-bb2cf1272d0d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_4a59007e-9957-43df-a396-93a533d780fa" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_3856addc-2524-41c0-af6a-bb2cf1272d0d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_e3bf7559-b566-42b7-ac8d-06b603976299" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_4a59007e-9957-43df-a396-93a533d780fa" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_e3bf7559-b566-42b7-ac8d-06b603976299" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesIssuedForServices_eee8c394-284f-4b3f-bb73-2c120e12dd1b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesIssuedForServices"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_4a59007e-9957-43df-a396-93a533d780fa" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesIssuedForServices_eee8c394-284f-4b3f-bb73-2c120e12dd1b" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardGrantsInPeriodWeightedAverageGrantDateFairValue_93b027c1-53f2-424a-894f-f8734a39fdb6" xlink:href="efsc-20221231.xsd#efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardGrantsInPeriodWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_4a59007e-9957-43df-a396-93a533d780fa" xlink:to="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardGrantsInPeriodWeightedAverageGrantDateFairValue_93b027c1-53f2-424a-894f-f8734a39fdb6" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_02093968-4fb3-4da8-bde7-18087e828eb3" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_4a59007e-9957-43df-a396-93a533d780fa" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_02093968-4fb3-4da8-bde7-18087e828eb3" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameAxis_bf4518c1-6d48-4e40-a89f-0e711912d22d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PlanNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_02093968-4fb3-4da8-bde7-18087e828eb3" xlink:to="loc_us-gaap_PlanNameAxis_bf4518c1-6d48-4e40-a89f-0e711912d22d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_bf4518c1-6d48-4e40-a89f-0e711912d22d_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PlanNameAxis_bf4518c1-6d48-4e40-a89f-0e711912d22d" xlink:to="loc_us-gaap_PlanNameDomain_bf4518c1-6d48-4e40-a89f-0e711912d22d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_d1e1e893-7141-40a8-ab19-89af66b8a921" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PlanNameAxis_bf4518c1-6d48-4e40-a89f-0e711912d22d" xlink:to="loc_us-gaap_PlanNameDomain_d1e1e893-7141-40a8-ab19-89af66b8a921" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_StockPlanForNonManagementDirectorsMember_ccd2c2cf-afec-4fbb-a183-85fc795c818c" xlink:href="efsc-20221231.xsd#efsc_StockPlanForNonManagementDirectorsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_d1e1e893-7141-40a8-ab19-89af66b8a921" xlink:to="loc_efsc_StockPlanForNonManagementDirectorsMember_ccd2c2cf-afec-4fbb-a183-85fc795c818c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_1b65dcdf-28bc-4a8c-bd14-dc8ea60a5844" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_02093968-4fb3-4da8-bde7-18087e828eb3" xlink:to="loc_us-gaap_AwardTypeAxis_1b65dcdf-28bc-4a8c-bd14-dc8ea60a5844" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_1b65dcdf-28bc-4a8c-bd14-dc8ea60a5844_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_1b65dcdf-28bc-4a8c-bd14-dc8ea60a5844" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_1b65dcdf-28bc-4a8c-bd14-dc8ea60a5844_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_25d86ff6-c959-48da-8bc6-051300747688" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_1b65dcdf-28bc-4a8c-bd14-dc8ea60a5844" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_25d86ff6-c959-48da-8bc6-051300747688" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockMember_7629e9ad-c6b3-4d1b-a7c4-a06e2b5003ac" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EmployeeStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_25d86ff6-c959-48da-8bc6-051300747688" xlink:to="loc_us-gaap_EmployeeStockMember_7629e9ad-c6b3-4d1b-a7c4-a06e2b5003ac" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_7f143c09-8168-4d57-8d6a-59f3f251ee02" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_25d86ff6-c959-48da-8bc6-051300747688" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_7f143c09-8168-4d57-8d6a-59f3f251ee02" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_StockOptionsAndStockSettledStockAppreciationRightsMember_76a30450-56a2-480e-a04a-58c11a70336a" xlink:href="efsc-20221231.xsd#efsc_StockOptionsAndStockSettledStockAppreciationRightsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_25d86ff6-c959-48da-8bc6-051300747688" xlink:to="loc_efsc_StockOptionsAndStockSettledStockAppreciationRightsMember_76a30450-56a2-480e-a04a-58c11a70336a" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/CompensationPlansWeightedAverageAssumptionsDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#CompensationPlansWeightedAverageAssumptionsDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/CompensationPlansWeightedAverageAssumptionsDetails" xlink:type="extended" id="i4b5dd01eb2704eeab4181bcd195aabe4_CompensationPlansWeightedAverageAssumptionsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_65c97134-985b-4e97-bb15-28b33271ae14" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate_f4f7b5bf-30f1-4301-8748-c8497c8600a9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_65c97134-985b-4e97-bb15-28b33271ae14" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate_f4f7b5bf-30f1-4301-8748-c8497c8600a9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate_68975ae2-9c0f-4fe0-b5a2-6b882a2b3f28" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_65c97134-985b-4e97-bb15-28b33271ae14" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate_68975ae2-9c0f-4fe0-b5a2-6b882a2b3f28" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate_28f223a3-5042-415b-a24c-7243a4fff396" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_65c97134-985b-4e97-bb15-28b33271ae14" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate_28f223a3-5042-415b-a24c-7243a4fff396" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1_76ffe10d-1b61-4e68-8eef-ce933cf7bd7e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_65c97134-985b-4e97-bb15-28b33271ae14" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1_76ffe10d-1b61-4e68-8eef-ce933cf7bd7e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_fcabaed1-9f07-4a73-bebb-9d03b8f62b8b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_65c97134-985b-4e97-bb15-28b33271ae14" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_fcabaed1-9f07-4a73-bebb-9d03b8f62b8b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_b070d23d-a1f0-4cf5-87a3-db2bff27680e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_fcabaed1-9f07-4a73-bebb-9d03b8f62b8b" xlink:to="loc_us-gaap_AwardTypeAxis_b070d23d-a1f0-4cf5-87a3-db2bff27680e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_b070d23d-a1f0-4cf5-87a3-db2bff27680e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_b070d23d-a1f0-4cf5-87a3-db2bff27680e" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_b070d23d-a1f0-4cf5-87a3-db2bff27680e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_d092b1bc-876f-446d-a35e-c8b98eb9e48d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_b070d23d-a1f0-4cf5-87a3-db2bff27680e" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_d092b1bc-876f-446d-a35e-c8b98eb9e48d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockOptionMember_bcfe1dd8-4b06-4ccb-936f-7b82bea9cb0f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EmployeeStockOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_d092b1bc-876f-446d-a35e-c8b98eb9e48d" xlink:to="loc_us-gaap_EmployeeStockOptionMember_bcfe1dd8-4b06-4ccb-936f-7b82bea9cb0f" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/CompensationPlansScheduleofEmployeeStockOptionsandSSARsActivityDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#CompensationPlansScheduleofEmployeeStockOptionsandSSARsActivityDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/CompensationPlansScheduleofEmployeeStockOptionsandSSARsActivityDetails" xlink:type="extended" id="id541ea87f7bf40e6bfc65c4527e43390_CompensationPlansScheduleofEmployeeStockOptionsandSSARsActivityDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_23b6e278-d1c9-4f69-87d6-3bfcb11da2f6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward_b119a193-b237-47b2-8318-37ff42f2a91f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_23b6e278-d1c9-4f69-87d6-3bfcb11da2f6" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward_b119a193-b237-47b2-8318-37ff42f2a91f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardOutstandingNumber_5a27fc12-bad5-4bcc-a875-4ff3d3051869" xlink:href="efsc-20221231.xsd#efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardOutstandingNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward_b119a193-b237-47b2-8318-37ff42f2a91f" xlink:to="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardOutstandingNumber_5a27fc12-bad5-4bcc-a875-4ff3d3051869" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardGrantsInPeriodGross_7fef6e25-c4e6-4b2c-8da3-d399b7712d1e" xlink:href="efsc-20221231.xsd#efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardGrantsInPeriodGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward_b119a193-b237-47b2-8318-37ff42f2a91f" xlink:to="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardGrantsInPeriodGross_7fef6e25-c4e6-4b2c-8da3-d399b7712d1e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardExercisesInPeriod_cf76fc95-8ba8-470e-a08b-bac203b660c2" xlink:href="efsc-20221231.xsd#efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardExercisesInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward_b119a193-b237-47b2-8318-37ff42f2a91f" xlink:to="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardExercisesInPeriod_cf76fc95-8ba8-470e-a08b-bac203b660c2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardForfeituresInPeriod_6fbcb054-d702-42db-9193-e1085c7ba381" xlink:href="efsc-20221231.xsd#efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardForfeituresInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward_b119a193-b237-47b2-8318-37ff42f2a91f" xlink:to="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardForfeituresInPeriod_6fbcb054-d702-42db-9193-e1085c7ba381" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardOutstandingNumber_b6679d1d-d0ea-45ff-899c-6bb598ae7025" xlink:href="efsc-20221231.xsd#efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardOutstandingNumber"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardExercisableNumber_63aa05ef-76fb-4546-a360-261f02f3bfd9" xlink:href="efsc-20221231.xsd#efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardExercisableNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingRollForward_b119a193-b237-47b2-8318-37ff42f2a91f" xlink:to="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardExercisableNumber_63aa05ef-76fb-4546-a360-261f02f3bfd9" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward_6a7590bd-aef8-4eef-8189-c3f3460fbd79" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_23b6e278-d1c9-4f69-87d6-3bfcb11da2f6" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward_6a7590bd-aef8-4eef-8189-c3f3460fbd79" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardOutstandingWeightedAverageExercisePrice_55fc35fe-d38c-4872-8219-5bf456c9d195" xlink:href="efsc-20221231.xsd#efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardOutstandingWeightedAverageExercisePrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward_6a7590bd-aef8-4eef-8189-c3f3460fbd79" xlink:to="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardOutstandingWeightedAverageExercisePrice_55fc35fe-d38c-4872-8219-5bf456c9d195" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardGrantsInPeriodWeightedAverageExercisePrice_f4222ae1-df60-4470-b354-db66afa2a1a6" xlink:href="efsc-20221231.xsd#efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardGrantsInPeriodWeightedAverageExercisePrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward_6a7590bd-aef8-4eef-8189-c3f3460fbd79" xlink:to="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardGrantsInPeriodWeightedAverageExercisePrice_f4222ae1-df60-4470-b354-db66afa2a1a6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardExercisesInPeriodWeightedAverageExercisePrice_dc8e8388-4d0c-4d22-a94a-1b45eccbe077" xlink:href="efsc-20221231.xsd#efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardExercisesInPeriodWeightedAverageExercisePrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward_6a7590bd-aef8-4eef-8189-c3f3460fbd79" xlink:to="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardExercisesInPeriodWeightedAverageExercisePrice_dc8e8388-4d0c-4d22-a94a-1b45eccbe077" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardForfeituresInPeriodWeightedAverageExercisePrice_b0e6ed5a-fc65-4d18-b0d2-336052501aac" xlink:href="efsc-20221231.xsd#efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardForfeituresInPeriodWeightedAverageExercisePrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward_6a7590bd-aef8-4eef-8189-c3f3460fbd79" xlink:to="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardForfeituresInPeriodWeightedAverageExercisePrice_b0e6ed5a-fc65-4d18-b0d2-336052501aac" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardOutstandingWeightedAverageExercisePrice_3b31c57e-70d3-48c4-a428-d24d350ef0ca" xlink:href="efsc-20221231.xsd#efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardOutstandingWeightedAverageExercisePrice"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardExercisableWeightedAverageExercisePrice_36daec06-cf1a-401a-aab5-d1dce45c65f1" xlink:href="efsc-20221231.xsd#efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardExercisableWeightedAverageExercisePrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePriceRollforward_6a7590bd-aef8-4eef-8189-c3f3460fbd79" xlink:to="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardExercisableWeightedAverageExercisePrice_36daec06-cf1a-401a-aab5-d1dce45c65f1" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsAdditionalDisclosuresAbstract_da31105a-9940-4295-8b36-e9603dac2518" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsAdditionalDisclosuresAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_23b6e278-d1c9-4f69-87d6-3bfcb11da2f6" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsAdditionalDisclosuresAbstract_da31105a-9940-4295-8b36-e9603dac2518" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardOutstandingWeightedAverageRemainingContractualTerm_2e74683b-24a4-4955-b392-4b4901ecbf0b" xlink:href="efsc-20221231.xsd#efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardOutstandingWeightedAverageRemainingContractualTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsAdditionalDisclosuresAbstract_da31105a-9940-4295-8b36-e9603dac2518" xlink:to="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardOutstandingWeightedAverageRemainingContractualTerm_2e74683b-24a4-4955-b392-4b4901ecbf0b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardExercisableWeightedAverageRemainingContractualTerm_686d03ae-1598-441c-a36d-0a9d6d0da712" xlink:href="efsc-20221231.xsd#efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardExercisableWeightedAverageRemainingContractualTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsAdditionalDisclosuresAbstract_da31105a-9940-4295-8b36-e9603dac2518" xlink:to="loc_efsc_ShareBasedCompensationArrangementByShareBasedPaymentAwardExercisableWeightedAverageRemainingContractualTerm_686d03ae-1598-441c-a36d-0a9d6d0da712" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_c0ad0a3b-9e76-42f8-82cd-3c39e80afa3c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_23b6e278-d1c9-4f69-87d6-3bfcb11da2f6" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_c0ad0a3b-9e76-42f8-82cd-3c39e80afa3c" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_471928a5-176c-45eb-8c42-02eb5f145d93" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_c0ad0a3b-9e76-42f8-82cd-3c39e80afa3c" xlink:to="loc_us-gaap_AwardTypeAxis_471928a5-176c-45eb-8c42-02eb5f145d93" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_471928a5-176c-45eb-8c42-02eb5f145d93_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_471928a5-176c-45eb-8c42-02eb5f145d93" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_471928a5-176c-45eb-8c42-02eb5f145d93_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_6b55b3ae-8989-4e06-8c51-8fa4c203e2a5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_471928a5-176c-45eb-8c42-02eb5f145d93" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_6b55b3ae-8989-4e06-8c51-8fa4c203e2a5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_StockOptionsAndStockSettledStockAppreciationRightsMember_d4a51db0-588b-4982-960c-d05e5b5ca8ff" xlink:href="efsc-20221231.xsd#efsc_StockOptionsAndStockSettledStockAppreciationRightsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_6b55b3ae-8989-4e06-8c51-8fa4c203e2a5" xlink:to="loc_efsc_StockOptionsAndStockSettledStockAppreciationRightsMember_d4a51db0-588b-4982-960c-d05e5b5ca8ff" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/CompensationPlansSummaryofRestrictedStockUnitsActivityDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#CompensationPlansSummaryofRestrictedStockUnitsActivityDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/CompensationPlansSummaryofRestrictedStockUnitsActivityDetails" xlink:type="extended" id="i9f3db6154eff45ed905da16d130d0f0b_CompensationPlansSummaryofRestrictedStockUnitsActivityDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_748733cb-597b-49c5-940d-ae91d1d2c58f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodTotalFairValue_457bfe1c-114b-408d-a135-6621ae9076a9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodTotalFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_748733cb-597b-49c5-940d-ae91d1d2c58f" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodTotalFairValue_457bfe1c-114b-408d-a135-6621ae9076a9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_72ad3031-e346-4bb0-adee-0d7b83146b09" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_748733cb-597b-49c5-940d-ae91d1d2c58f" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_72ad3031-e346-4bb0-adee-0d7b83146b09" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_8182b9bf-f749-4ab8-8a6c-06ce3c73e026" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_72ad3031-e346-4bb0-adee-0d7b83146b09" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_8182b9bf-f749-4ab8-8a6c-06ce3c73e026" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod_0dad61d8-8ca6-40f3-b485-ca8494cd29ef" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_72ad3031-e346-4bb0-adee-0d7b83146b09" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod_0dad61d8-8ca6-40f3-b485-ca8494cd29ef" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod_1276261e-65d7-400f-8e43-c63793f35733" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_72ad3031-e346-4bb0-adee-0d7b83146b09" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod_1276261e-65d7-400f-8e43-c63793f35733" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeitedInPeriod_a5650bc3-5960-4c1d-9dd1-228d380728a7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeitedInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedRollForward_72ad3031-e346-4bb0-adee-0d7b83146b09" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeitedInPeriod_a5650bc3-5960-4c1d-9dd1-228d380728a7" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_2914d7f3-243c-4031-b4d6-f2fbbc4017b0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_0e88c9fc-e3b7-4335-9a65-c964ce375083" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_748733cb-597b-49c5-940d-ae91d1d2c58f" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_0e88c9fc-e3b7-4335-9a65-c964ce375083" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue_7b249171-4b30-4aa6-890b-3659f77714e3" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_0e88c9fc-e3b7-4335-9a65-c964ce375083" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue_7b249171-4b30-4aa6-890b-3659f77714e3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue_60e83bd9-1154-4a26-86f1-6302cea09eec" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_0e88c9fc-e3b7-4335-9a65-c964ce375083" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue_60e83bd9-1154-4a26-86f1-6302cea09eec" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodWeightedAverageGrantDateFairValue_263621f8-33b1-4b6a-947a-80e49f2781c4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_0e88c9fc-e3b7-4335-9a65-c964ce375083" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodWeightedAverageGrantDateFairValue_263621f8-33b1-4b6a-947a-80e49f2781c4" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeituresWeightedAverageGrantDateFairValue_24d4b8bf-9acc-4ed3-9617-d974ddd66fd1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeituresWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValueRollForward_0e88c9fc-e3b7-4335-9a65-c964ce375083" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeituresWeightedAverageGrantDateFairValue_24d4b8bf-9acc-4ed3-9617-d974ddd66fd1" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue_41321711-1041-45f2-9350-e41f2ccbd64c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_7b1eb396-d074-4a6f-9803-5ecf2bacb3bd" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_748733cb-597b-49c5-940d-ae91d1d2c58f" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_7b1eb396-d074-4a6f-9803-5ecf2bacb3bd" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_75c897ad-532e-442a-8647-3b05dbeda561" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_748733cb-597b-49c5-940d-ae91d1d2c58f" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_75c897ad-532e-442a-8647-3b05dbeda561" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageGrantDateFairValue_40a902a8-9b1e-4516-99c4-cf13eecac853" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_748733cb-597b-49c5-940d-ae91d1d2c58f" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageGrantDateFairValue_40a902a8-9b1e-4516-99c4-cf13eecac853" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_d23d39e1-d439-438c-9f5a-901f66870195" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_748733cb-597b-49c5-940d-ae91d1d2c58f" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_d23d39e1-d439-438c-9f5a-901f66870195" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_9f713633-6ea9-4ad2-ab8e-86cce6dfd035" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_d23d39e1-d439-438c-9f5a-901f66870195" xlink:to="loc_us-gaap_AwardTypeAxis_9f713633-6ea9-4ad2-ab8e-86cce6dfd035" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_9f713633-6ea9-4ad2-ab8e-86cce6dfd035_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_9f713633-6ea9-4ad2-ab8e-86cce6dfd035" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_9f713633-6ea9-4ad2-ab8e-86cce6dfd035_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_0a71d912-08cf-4c0d-bc9f-a2b2608c2e13" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_9f713633-6ea9-4ad2-ab8e-86cce6dfd035" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_0a71d912-08cf-4c0d-bc9f-a2b2608c2e13" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_0a9a6278-b7fe-45ca-8a49-8cd111ed2165" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_0a71d912-08cf-4c0d-bc9f-a2b2608c2e13" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_0a9a6278-b7fe-45ca-8a49-8cd111ed2165" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PerformanceSharesMember_4a90b08c-b193-4693-b7b5-d96b53ee4649" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PerformanceSharesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_0a71d912-08cf-4c0d-bc9f-a2b2608c2e13" xlink:to="loc_us-gaap_PerformanceSharesMember_4a90b08c-b193-4693-b7b5-d96b53ee4649" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/IncomeTaxesNarrativeDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#IncomeTaxesNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/IncomeTaxesNarrativeDetails" xlink:type="extended" id="id010f11f02af4d79a7dfb746bab6ceac_IncomeTaxesNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TaxCreditCarryforwardLineItems_2eea3c0a-77ca-4bf1-bd93-6553899dc394" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TaxCreditCarryforwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_Incometaxbenefitlowincomehousingtaxcredits_323b44ed-b28e-445b-8cd4-05e78ab78379" xlink:href="efsc-20221231.xsd#efsc_Incometaxbenefitlowincomehousingtaxcredits"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TaxCreditCarryforwardLineItems_2eea3c0a-77ca-4bf1-bd93-6553899dc394" xlink:to="loc_efsc_Incometaxbenefitlowincomehousingtaxcredits_323b44ed-b28e-445b-8cd4-05e78ab78379" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_Investmentslowincomehousingtaxcredits_e014ed28-a724-4080-8883-a1a87c584844" xlink:href="efsc-20221231.xsd#efsc_Investmentslowincomehousingtaxcredits"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TaxCreditCarryforwardLineItems_2eea3c0a-77ca-4bf1-bd93-6553899dc394" xlink:to="loc_efsc_Investmentslowincomehousingtaxcredits_e014ed28-a724-4080-8883-a1a87c584844" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsTaxDeferredExpenseReservesAndAccrualsImpairmentLosses_ab0351ea-3a28-44a1-8cbf-87aa698e0a96" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DeferredTaxAssetsTaxDeferredExpenseReservesAndAccrualsImpairmentLosses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TaxCreditCarryforwardLineItems_2eea3c0a-77ca-4bf1-bd93-6553899dc394" xlink:to="loc_us-gaap_DeferredTaxAssetsTaxDeferredExpenseReservesAndAccrualsImpairmentLosses_ab0351ea-3a28-44a1-8cbf-87aa698e0a96" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsLiabilitiesNet_de6d2c71-9738-474b-87b9-6c676453cfa2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DeferredTaxAssetsLiabilitiesNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TaxCreditCarryforwardLineItems_2eea3c0a-77ca-4bf1-bd93-6553899dc394" xlink:to="loc_us-gaap_DeferredTaxAssetsLiabilitiesNet_de6d2c71-9738-474b-87b9-6c676453cfa2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsValuationAllowance_286c2431-2161-4f4e-8cbe-a672298ab336" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DeferredTaxAssetsValuationAllowance"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TaxCreditCarryforwardLineItems_2eea3c0a-77ca-4bf1-bd93-6553899dc394" xlink:to="loc_us-gaap_DeferredTaxAssetsValuationAllowance_286c2431-2161-4f4e-8cbe-a672298ab336" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_NumberOfStatesCompanyFilesIncomeTaxReturnsWith_0f301cb6-620e-4080-aad3-4a7058ff4d00" xlink:href="efsc-20221231.xsd#efsc_NumberOfStatesCompanyFilesIncomeTaxReturnsWith"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TaxCreditCarryforwardLineItems_2eea3c0a-77ca-4bf1-bd93-6553899dc394" xlink:to="loc_efsc_NumberOfStatesCompanyFilesIncomeTaxReturnsWith_0f301cb6-620e-4080-aad3-4a7058ff4d00" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnrecognizedTaxBenefits_e73ea7ee-bf03-4984-830b-d73877364104" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_UnrecognizedTaxBenefits"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TaxCreditCarryforwardLineItems_2eea3c0a-77ca-4bf1-bd93-6553899dc394" xlink:to="loc_us-gaap_UnrecognizedTaxBenefits_e73ea7ee-bf03-4984-830b-d73877364104" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnrecognizedTaxBenefitsThatWouldImpactEffectiveTaxRate_11e7b861-5f0d-496a-a7e4-d203d1580e79" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_UnrecognizedTaxBenefitsThatWouldImpactEffectiveTaxRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TaxCreditCarryforwardLineItems_2eea3c0a-77ca-4bf1-bd93-6553899dc394" xlink:to="loc_us-gaap_UnrecognizedTaxBenefitsThatWouldImpactEffectiveTaxRate_11e7b861-5f0d-496a-a7e4-d203d1580e79" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SignificantChangeInUnrecognizedTaxBenefitsIsReasonablyPossibleAmountOfUnrecordedBenefit_24eab8c3-80a8-41ce-8361-35b4b847321a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SignificantChangeInUnrecognizedTaxBenefitsIsReasonablyPossibleAmountOfUnrecordedBenefit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TaxCreditCarryforwardLineItems_2eea3c0a-77ca-4bf1-bd93-6553899dc394" xlink:to="loc_us-gaap_SignificantChangeInUnrecognizedTaxBenefitsIsReasonablyPossibleAmountOfUnrecordedBenefit_24eab8c3-80a8-41ce-8361-35b4b847321a" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_Durationofunrecognizedtaxbenefits_da7ee42d-c085-4877-a287-969e1e3126c1" xlink:href="efsc-20221231.xsd#efsc_Durationofunrecognizedtaxbenefits"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TaxCreditCarryforwardLineItems_2eea3c0a-77ca-4bf1-bd93-6553899dc394" xlink:to="loc_efsc_Durationofunrecognizedtaxbenefits_da7ee42d-c085-4877-a287-969e1e3126c1" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExaminationPenaltiesAndInterestAccrued_ef10fd7c-036e-4e12-bf9d-5dbdc40d9c24" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeTaxExaminationPenaltiesAndInterestAccrued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TaxCreditCarryforwardLineItems_2eea3c0a-77ca-4bf1-bd93-6553899dc394" xlink:to="loc_us-gaap_IncomeTaxExaminationPenaltiesAndInterestAccrued_ef10fd7c-036e-4e12-bf9d-5dbdc40d9c24" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TaxCreditCarryforwardTable_4f4a22de-fe8e-486a-8c12-385fd33af93d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TaxCreditCarryforwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_TaxCreditCarryforwardLineItems_2eea3c0a-77ca-4bf1-bd93-6553899dc394" xlink:to="loc_us-gaap_TaxCreditCarryforwardTable_4f4a22de-fe8e-486a-8c12-385fd33af93d" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BalanceSheetLocationAxis_9d435295-4883-4ecc-9e93-00969a04ea2e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_BalanceSheetLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_TaxCreditCarryforwardTable_4f4a22de-fe8e-486a-8c12-385fd33af93d" xlink:to="loc_us-gaap_BalanceSheetLocationAxis_9d435295-4883-4ecc-9e93-00969a04ea2e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BalanceSheetLocationDomain_9d435295-4883-4ecc-9e93-00969a04ea2e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_BalanceSheetLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BalanceSheetLocationAxis_9d435295-4883-4ecc-9e93-00969a04ea2e" xlink:to="loc_us-gaap_BalanceSheetLocationDomain_9d435295-4883-4ecc-9e93-00969a04ea2e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BalanceSheetLocationDomain_33d2c905-17bd-4d9b-a1da-fd0ba84dab6e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_BalanceSheetLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BalanceSheetLocationAxis_9d435295-4883-4ecc-9e93-00969a04ea2e" xlink:to="loc_us-gaap_BalanceSheetLocationDomain_33d2c905-17bd-4d9b-a1da-fd0ba84dab6e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsMember_95fb97ee-5669-44a9-9131-ace964e0df8d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BalanceSheetLocationDomain_33d2c905-17bd-4d9b-a1da-fd0ba84dab6e" xlink:to="loc_us-gaap_OtherAssetsMember_95fb97ee-5669-44a9-9131-ace964e0df8d" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/CommitmentsDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#CommitmentsDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/CommitmentsDetails" xlink:type="extended" id="if3a35429ced9488ca99989fb089fb825_CommitmentsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ScheduleOfCommitmentsLineItems_88d3495c-414e-47c9-840b-bbe3a90a31b0" xlink:href="efsc-20221231.xsd#efsc_ScheduleOfCommitmentsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansAndLeasesReceivableImpairedLoansCommitmentToLendReserve_454f77c1-2375-40e1-a515-f291601c1d53" xlink:href="efsc-20221231.xsd#efsc_LoansAndLeasesReceivableImpairedLoansCommitmentToLendReserve"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_efsc_ScheduleOfCommitmentsLineItems_88d3495c-414e-47c9-840b-bbe3a90a31b0" xlink:to="loc_efsc_LoansAndLeasesReceivableImpairedLoansCommitmentToLendReserve_454f77c1-2375-40e1-a515-f291601c1d53" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ContractualAmountsOfOffBalanceSheetFinancialInstruments_ec1b67d2-d619-4ccd-a136-62062c87f6ac" xlink:href="efsc-20221231.xsd#efsc_ContractualAmountsOfOffBalanceSheetFinancialInstruments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_efsc_ScheduleOfCommitmentsLineItems_88d3495c-414e-47c9-840b-bbe3a90a31b0" xlink:to="loc_efsc_ContractualAmountsOfOffBalanceSheetFinancialInstruments_ec1b67d2-d619-4ccd-a136-62062c87f6ac" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_DebtInstrumentRemainingTerm_c1f6b6e8-1cde-4002-81dd-d36b2970a64f" xlink:href="efsc-20221231.xsd#efsc_DebtInstrumentRemainingTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_efsc_ScheduleOfCommitmentsLineItems_88d3495c-414e-47c9-840b-bbe3a90a31b0" xlink:to="loc_efsc_DebtInstrumentRemainingTerm_c1f6b6e8-1cde-4002-81dd-d36b2970a64f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ScheduleOfCommitmentsTable_f95d823d-6368-4751-bca2-7118d76f71d6" xlink:href="efsc-20221231.xsd#efsc_ScheduleOfCommitmentsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_efsc_ScheduleOfCommitmentsLineItems_88d3495c-414e-47c9-840b-bbe3a90a31b0" xlink:to="loc_efsc_ScheduleOfCommitmentsTable_f95d823d-6368-4751-bca2-7118d76f71d6" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_NatureOfCommitmentsAxis_eb429c0a-7be5-4f9c-9dee-0b8f241f96a5" xlink:href="efsc-20221231.xsd#efsc_NatureOfCommitmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_efsc_ScheduleOfCommitmentsTable_f95d823d-6368-4751-bca2-7118d76f71d6" xlink:to="loc_efsc_NatureOfCommitmentsAxis_eb429c0a-7be5-4f9c-9dee-0b8f241f96a5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_NatureOfCommitmentsDomain_eb429c0a-7be5-4f9c-9dee-0b8f241f96a5_default" xlink:href="efsc-20221231.xsd#efsc_NatureOfCommitmentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_efsc_NatureOfCommitmentsAxis_eb429c0a-7be5-4f9c-9dee-0b8f241f96a5" xlink:to="loc_efsc_NatureOfCommitmentsDomain_eb429c0a-7be5-4f9c-9dee-0b8f241f96a5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_NatureOfCommitmentsDomain_deb9aae9-2ac4-4f90-907a-0e861bcd418b" xlink:href="efsc-20221231.xsd#efsc_NatureOfCommitmentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_efsc_NatureOfCommitmentsAxis_eb429c0a-7be5-4f9c-9dee-0b8f241f96a5" xlink:to="loc_efsc_NatureOfCommitmentsDomain_deb9aae9-2ac4-4f90-907a-0e861bcd418b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_UnadvancedCommitmentOnImpairedLoanMember_7d03a03f-0396-4c9e-9926-30d896ecd833" xlink:href="efsc-20221231.xsd#efsc_UnadvancedCommitmentOnImpairedLoanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_efsc_NatureOfCommitmentsDomain_deb9aae9-2ac4-4f90-907a-0e861bcd418b" xlink:to="loc_efsc_UnadvancedCommitmentOnImpairedLoanMember_7d03a03f-0396-4c9e-9926-30d896ecd833" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_FixedRateLoanCommitmentMember_11e7da6f-7794-4a3f-9c9d-53b531753e3f" xlink:href="efsc-20221231.xsd#efsc_FixedRateLoanCommitmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_efsc_NatureOfCommitmentsDomain_deb9aae9-2ac4-4f90-907a-0e861bcd418b" xlink:to="loc_efsc_FixedRateLoanCommitmentMember_11e7da6f-7794-4a3f-9c9d-53b531753e3f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_6ca27c53-087a-4a11-bd72-ace212aff7b8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_efsc_ScheduleOfCommitmentsTable_f95d823d-6368-4751-bca2-7118d76f71d6" xlink:to="loc_us-gaap_FinancialInstrumentAxis_6ca27c53-087a-4a11-bd72-ace212aff7b8" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_6ca27c53-087a-4a11-bd72-ace212aff7b8_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_6ca27c53-087a-4a11-bd72-ace212aff7b8" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_6ca27c53-087a-4a11-bd72-ace212aff7b8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_0d31aeda-0ebd-425e-864f-18345033f588" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_6ca27c53-087a-4a11-bd72-ace212aff7b8" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_0d31aeda-0ebd-425e-864f-18345033f588" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommitmentsToExtendCreditMember_5f1dd943-17dc-414b-beaa-701c1981dbf3" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CommitmentsToExtendCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_0d31aeda-0ebd-425e-864f-18345033f588" xlink:to="loc_us-gaap_CommitmentsToExtendCreditMember_5f1dd943-17dc-414b-beaa-701c1981dbf3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StandbyLettersOfCreditMember_1654d60b-3820-419f-8505-6ff9242a2e85" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StandbyLettersOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_0d31aeda-0ebd-425e-864f-18345033f588" xlink:to="loc_us-gaap_StandbyLettersOfCreditMember_1654d60b-3820-419f-8505-6ff9242a2e85" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_StateTaxCreditsHeldForSaleMember_767c72ea-e67c-474e-84dd-faa9149a63e5" xlink:href="efsc-20221231.xsd#efsc_StateTaxCreditsHeldForSaleMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_0d31aeda-0ebd-425e-864f-18345033f588" xlink:to="loc_efsc_StateTaxCreditsHeldForSaleMember_767c72ea-e67c-474e-84dd-faa9149a63e5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_SBICCapitalCommitmentsDomain_573f49f6-4766-4b3b-975b-d934a3b26ee1" xlink:href="efsc-20221231.xsd#efsc_SBICCapitalCommitmentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_0d31aeda-0ebd-425e-864f-18345033f588" xlink:to="loc_efsc_SBICCapitalCommitmentsDomain_573f49f6-4766-4b3b-975b-d934a3b26ee1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_2003f8b2-b155-4694-b937-2d21ffdc8d03" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_efsc_ScheduleOfCommitmentsTable_f95d823d-6368-4751-bca2-7118d76f71d6" xlink:to="loc_srt_RangeAxis_2003f8b2-b155-4694-b937-2d21ffdc8d03" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_2003f8b2-b155-4694-b937-2d21ffdc8d03_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_2003f8b2-b155-4694-b937-2d21ffdc8d03" xlink:to="loc_srt_RangeMember_2003f8b2-b155-4694-b937-2d21ffdc8d03_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_e588d298-9700-4f57-a448-e37030cc34f2" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_2003f8b2-b155-4694-b937-2d21ffdc8d03" xlink:to="loc_srt_RangeMember_e588d298-9700-4f57-a448-e37030cc34f2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_61e42277-b271-42ad-9917-fd1ac33934cf" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_e588d298-9700-4f57-a448-e37030cc34f2" xlink:to="loc_srt_MinimumMember_61e42277-b271-42ad-9917-fd1ac33934cf" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_3a3cc0c6-ae61-4dcd-94e7-a33221349dda" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_e588d298-9700-4f57-a448-e37030cc34f2" xlink:to="loc_srt_MaximumMember_3a3cc0c6-ae61-4dcd-94e7-a33221349dda" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/FairValueMeasurementsSummaryofFinancialInstrumentsMeasuredatFairValueonaRecurringBasisDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#FairValueMeasurementsSummaryofFinancialInstrumentsMeasuredatFairValueonaRecurringBasisDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/FairValueMeasurementsSummaryofFinancialInstrumentsMeasuredatFairValueonaRecurringBasisDetails" xlink:type="extended" id="i5c65986a1c6c4eaba98d36599f589d13_FairValueMeasurementsSummaryofFinancialInstrumentsMeasuredatFairValueonaRecurringBasisDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_d072f0f1-dae8-4a73-a450-bb342d3dbfa3" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_5dc4f092-c482-4f16-b775-3d8ed4a6992f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_d072f0f1-dae8-4a73-a450-bb342d3dbfa3" xlink:to="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_5dc4f092-c482-4f16-b775-3d8ed4a6992f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_91441a04-74dd-40b2-b439-d948c9254b4f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AvailableForSaleSecuritiesDebtSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_5dc4f092-c482-4f16-b775-3d8ed4a6992f" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_91441a04-74dd-40b2-b439-d948c9254b4f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherInvestments_b9b19c41-1a72-44fd-b4aa-170ef946f61b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherInvestments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_5dc4f092-c482-4f16-b775-3d8ed4a6992f" xlink:to="loc_us-gaap_OtherInvestments_b9b19c41-1a72-44fd-b4aa-170ef946f61b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeAssets_7e656c6d-de81-4327-bb06-9a2b6f6f8bba" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_5dc4f092-c482-4f16-b775-3d8ed4a6992f" xlink:to="loc_us-gaap_DerivativeAssets_7e656c6d-de81-4327-bb06-9a2b6f6f8bba" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsFairValueDisclosure_d76d93cd-83ef-48b8-89d2-12df4465fbcf" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AssetsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_d072f0f1-dae8-4a73-a450-bb342d3dbfa3" xlink:to="loc_us-gaap_AssetsFairValueDisclosure_d76d93cd-83ef-48b8-89d2-12df4465fbcf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_db4aa726-3443-4d18-b419-e3176dc88604" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_d072f0f1-dae8-4a73-a450-bb342d3dbfa3" xlink:to="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_db4aa726-3443-4d18-b419-e3176dc88604" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeLiabilities_d6e5212a-2485-48d9-89b1-f243a5c2186e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DerivativeLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_db4aa726-3443-4d18-b419-e3176dc88604" xlink:to="loc_us-gaap_DerivativeLiabilities_d6e5212a-2485-48d9-89b1-f243a5c2186e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesFairValueDisclosure_6c409e73-01e7-4d65-b23f-87c8d4b7a28e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LiabilitiesFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_db4aa726-3443-4d18-b419-e3176dc88604" xlink:to="loc_us-gaap_LiabilitiesFairValueDisclosure_6c409e73-01e7-4d65-b23f-87c8d4b7a28e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_7621a21c-0aab-4556-a42b-e98d5e9ac5da" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_d072f0f1-dae8-4a73-a450-bb342d3dbfa3" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_7621a21c-0aab-4556-a42b-e98d5e9ac5da" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementFrequencyAxis_4aaf15d2-9f8c-42ef-a426-d1c201dcecf2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueByMeasurementFrequencyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_7621a21c-0aab-4556-a42b-e98d5e9ac5da" xlink:to="loc_us-gaap_FairValueByMeasurementFrequencyAxis_4aaf15d2-9f8c-42ef-a426-d1c201dcecf2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_4aaf15d2-9f8c-42ef-a426-d1c201dcecf2_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_4aaf15d2-9f8c-42ef-a426-d1c201dcecf2" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_4aaf15d2-9f8c-42ef-a426-d1c201dcecf2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_0421606e-814a-44e5-84c4-f17ea2e635b3" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_4aaf15d2-9f8c-42ef-a426-d1c201dcecf2" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_0421606e-814a-44e5-84c4-f17ea2e635b3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsRecurringMember_0e348bca-51c3-4127-8587-bf38a8130330" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueMeasurementsRecurringMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementFrequencyDomain_0421606e-814a-44e5-84c4-f17ea2e635b3" xlink:to="loc_us-gaap_FairValueMeasurementsRecurringMember_0e348bca-51c3-4127-8587-bf38a8130330" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_fcaa8c63-1ad8-48df-93e0-737c0f9df01b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_7621a21c-0aab-4556-a42b-e98d5e9ac5da" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_fcaa8c63-1ad8-48df-93e0-737c0f9df01b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_fcaa8c63-1ad8-48df-93e0-737c0f9df01b_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_fcaa8c63-1ad8-48df-93e0-737c0f9df01b" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_fcaa8c63-1ad8-48df-93e0-737c0f9df01b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_4c8200c3-982d-4895-a059-3ae9b04e85c4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_fcaa8c63-1ad8-48df-93e0-737c0f9df01b" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_4c8200c3-982d-4895-a059-3ae9b04e85c4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_0e72e69c-e87b-41bf-963a-bfa71bca5324" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_4c8200c3-982d-4895-a059-3ae9b04e85c4" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_0e72e69c-e87b-41bf-963a-bfa71bca5324" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_3d5f746b-a926-4e71-9c93-9c5914ef71e7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_4c8200c3-982d-4895-a059-3ae9b04e85c4" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_3d5f746b-a926-4e71-9c93-9c5914ef71e7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_b30f0491-b47f-474a-983e-a5e7aaee993b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueInputsLevel3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_4c8200c3-982d-4895-a059-3ae9b04e85c4" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_b30f0491-b47f-474a-983e-a5e7aaee993b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_c2efb70f-6c35-4eda-ae7b-e17a968699e1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_7621a21c-0aab-4556-a42b-e98d5e9ac5da" xlink:to="loc_us-gaap_FinancialInstrumentAxis_c2efb70f-6c35-4eda-ae7b-e17a968699e1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_c2efb70f-6c35-4eda-ae7b-e17a968699e1_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_c2efb70f-6c35-4eda-ae7b-e17a968699e1" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_c2efb70f-6c35-4eda-ae7b-e17a968699e1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_c3da02c8-a55a-4c8d-a0ac-3160c50e3352" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_c2efb70f-6c35-4eda-ae7b-e17a968699e1" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_c3da02c8-a55a-4c8d-a0ac-3160c50e3352" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USGovernmentSponsoredEnterprisesDebtSecuritiesMember_f521ecc5-0433-416f-80d2-364d4a20f3d9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_USGovernmentSponsoredEnterprisesDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_c3da02c8-a55a-4c8d-a0ac-3160c50e3352" xlink:to="loc_us-gaap_USGovernmentSponsoredEnterprisesDebtSecuritiesMember_f521ecc5-0433-416f-80d2-364d4a20f3d9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USStatesAndPoliticalSubdivisionsMember_f82bad84-c0f4-476d-8231-813f69748f24" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_USStatesAndPoliticalSubdivisionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_c3da02c8-a55a-4c8d-a0ac-3160c50e3352" xlink:to="loc_us-gaap_USStatesAndPoliticalSubdivisionsMember_f82bad84-c0f4-476d-8231-813f69748f24" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResidentialMortgageBackedSecuritiesMember_abf60ff5-00d1-48c2-8a97-51fcbe3842c5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ResidentialMortgageBackedSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_c3da02c8-a55a-4c8d-a0ac-3160c50e3352" xlink:to="loc_us-gaap_ResidentialMortgageBackedSecuritiesMember_abf60ff5-00d1-48c2-8a97-51fcbe3842c5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CorporateDebtSecuritiesMember_2073a17e-36a9-4d6a-ac1e-da32e107f4cb" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CorporateDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_c3da02c8-a55a-4c8d-a0ac-3160c50e3352" xlink:to="loc_us-gaap_CorporateDebtSecuritiesMember_2073a17e-36a9-4d6a-ac1e-da32e107f4cb" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USTreasuryBillSecuritiesMember_c0c59e82-8abd-4cce-a1a2-7715c76bb5e7" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_USTreasuryBillSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_c3da02c8-a55a-4c8d-a0ac-3160c50e3352" xlink:to="loc_us-gaap_USTreasuryBillSecuritiesMember_c0c59e82-8abd-4cce-a1a2-7715c76bb5e7" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementBasisAxis_ebfe40b4-1bbb-4cd2-acd6-489383aa7f37" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueByMeasurementBasisAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_7621a21c-0aab-4556-a42b-e98d5e9ac5da" xlink:to="loc_us-gaap_FairValueByMeasurementBasisAxis_ebfe40b4-1bbb-4cd2-acd6-489383aa7f37" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_ebfe40b4-1bbb-4cd2-acd6-489383aa7f37_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_ebfe40b4-1bbb-4cd2-acd6-489383aa7f37" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_ebfe40b4-1bbb-4cd2-acd6-489383aa7f37_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueDisclosureItemAmountsDomain_d05ae39b-2c34-4995-8092-7fea9cc55250" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueDisclosureItemAmountsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_ebfe40b4-1bbb-4cd2-acd6-489383aa7f37" xlink:to="loc_us-gaap_FairValueDisclosureItemAmountsDomain_d05ae39b-2c34-4995-8092-7fea9cc55250" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_20c8253f-e52b-4a83-8909-8db4e0cc2700" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueDisclosureItemAmountsDomain_d05ae39b-2c34-4995-8092-7fea9cc55250" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_20c8253f-e52b-4a83-8909-8db4e0cc2700" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EstimateOfFairValueFairValueDisclosureMember_d60dae3f-fd05-4193-bf40-7ae549c85c2c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EstimateOfFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_20c8253f-e52b-4a83-8909-8db4e0cc2700" xlink:to="loc_us-gaap_EstimateOfFairValueFairValueDisclosureMember_d60dae3f-fd05-4193-bf40-7ae549c85c2c" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/FairValueMeasurementsNarrativeDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#FairValueMeasurementsNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/FairValueMeasurementsNarrativeDetails" xlink:type="extended" id="i16b74b4c4fc7433bbf60b42a8406d95c_FairValueMeasurementsNarrativeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_25aa7ff3-8d06-4d13-8c27-906c48f7b55e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_StateTaxCreditsHeldForSaleFairValueDisclosure_90e271be-9989-40f4-8de9-e0815cf796ce" xlink:href="efsc-20221231.xsd#efsc_StateTaxCreditsHeldForSaleFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_25aa7ff3-8d06-4d13-8c27-906c48f7b55e" xlink:to="loc_efsc_StateTaxCreditsHeldForSaleFairValueDisclosure_90e271be-9989-40f4-8de9-e0815cf796ce" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_957724ed-0cf8-4d10-aa98-510d872cae76" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_25aa7ff3-8d06-4d13-8c27-906c48f7b55e" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_957724ed-0cf8-4d10-aa98-510d872cae76" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementBasisAxis_33c0f4c6-fe76-4d7e-838e-ffa4aa5bdcc1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueByMeasurementBasisAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_957724ed-0cf8-4d10-aa98-510d872cae76" xlink:to="loc_us-gaap_FairValueByMeasurementBasisAxis_33c0f4c6-fe76-4d7e-838e-ffa4aa5bdcc1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_33c0f4c6-fe76-4d7e-838e-ffa4aa5bdcc1_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_33c0f4c6-fe76-4d7e-838e-ffa4aa5bdcc1" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_33c0f4c6-fe76-4d7e-838e-ffa4aa5bdcc1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueDisclosureItemAmountsDomain_28227735-96d8-4174-95bb-e7127059a4aa" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueDisclosureItemAmountsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_33c0f4c6-fe76-4d7e-838e-ffa4aa5bdcc1" xlink:to="loc_us-gaap_FairValueDisclosureItemAmountsDomain_28227735-96d8-4174-95bb-e7127059a4aa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_84b1af9e-9139-4ee2-9b7f-57606c924291" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueDisclosureItemAmountsDomain_28227735-96d8-4174-95bb-e7127059a4aa" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_84b1af9e-9139-4ee2-9b7f-57606c924291" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EstimateOfFairValueFairValueDisclosureMember_d2de8c45-4300-40f4-af31-1a7b567ca49d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EstimateOfFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueDisclosureItemAmountsDomain_28227735-96d8-4174-95bb-e7127059a4aa" xlink:to="loc_us-gaap_EstimateOfFairValueFairValueDisclosureMember_d2de8c45-4300-40f4-af31-1a7b567ca49d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementFrequencyAxis_f55c9202-b844-48e9-b085-c2ceb0ca9bd4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueByMeasurementFrequencyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_957724ed-0cf8-4d10-aa98-510d872cae76" xlink:to="loc_us-gaap_FairValueByMeasurementFrequencyAxis_f55c9202-b844-48e9-b085-c2ceb0ca9bd4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_f55c9202-b844-48e9-b085-c2ceb0ca9bd4_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_f55c9202-b844-48e9-b085-c2ceb0ca9bd4" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_f55c9202-b844-48e9-b085-c2ceb0ca9bd4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_cbc44a56-e487-4b90-81f4-9677ca781f7e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_f55c9202-b844-48e9-b085-c2ceb0ca9bd4" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_cbc44a56-e487-4b90-81f4-9677ca781f7e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsRecurringMember_22d8c4b4-2df3-46d4-be34-20dc64a41ea8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueMeasurementsRecurringMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementFrequencyDomain_cbc44a56-e487-4b90-81f4-9677ca781f7e" xlink:to="loc_us-gaap_FairValueMeasurementsRecurringMember_22d8c4b4-2df3-46d4-be34-20dc64a41ea8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_effc9312-9d4b-473d-b6b4-18e728a20ad8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_957724ed-0cf8-4d10-aa98-510d872cae76" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_effc9312-9d4b-473d-b6b4-18e728a20ad8" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_effc9312-9d4b-473d-b6b4-18e728a20ad8_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_effc9312-9d4b-473d-b6b4-18e728a20ad8" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_effc9312-9d4b-473d-b6b4-18e728a20ad8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_acad05d8-83a7-49cb-9f4a-17e7c26beec2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_effc9312-9d4b-473d-b6b4-18e728a20ad8" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_acad05d8-83a7-49cb-9f4a-17e7c26beec2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_764a0779-cb7b-4cfe-af02-683b4bfb4902" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueInputsLevel3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_acad05d8-83a7-49cb-9f4a-17e7c26beec2" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_764a0779-cb7b-4cfe-af02-683b4bfb4902" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/FairValueMeasurementsSummaryofFinancialInstrumentsandNonFinancialAssetsMeasuredatFairValueonaNonRecurringBasisDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#FairValueMeasurementsSummaryofFinancialInstrumentsandNonFinancialAssetsMeasuredatFairValueonaNonRecurringBasisDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/FairValueMeasurementsSummaryofFinancialInstrumentsandNonFinancialAssetsMeasuredatFairValueonaNonRecurringBasisDetails" xlink:type="extended" id="i4daa77ced79942329981d7214d5d343a_FairValueMeasurementsSummaryofFinancialInstrumentsandNonFinancialAssetsMeasuredatFairValueonaNonRecurringBasisDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_8ac0d230-57db-4eab-b4a1-abd482369dac" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LoansReceivableFairValueDisclosure_94e14e50-4504-4b3f-b651-a1b9f5c308f5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LoansReceivableFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_8ac0d230-57db-4eab-b4a1-abd482369dac" xlink:to="loc_us-gaap_LoansReceivableFairValueDisclosure_94e14e50-4504-4b3f-b651-a1b9f5c308f5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_OtherRealEstateFairValueDisclosure_8ac8b0a7-fd38-4327-a192-96d518422075" xlink:href="efsc-20221231.xsd#efsc_OtherRealEstateFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_8ac0d230-57db-4eab-b4a1-abd482369dac" xlink:to="loc_efsc_OtherRealEstateFairValueDisclosure_8ac8b0a7-fd38-4327-a192-96d518422075" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsFairValueDisclosure_d309c150-4bc5-4947-aed8-1d3fffc09fb0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AssetsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_8ac0d230-57db-4eab-b4a1-abd482369dac" xlink:to="loc_us-gaap_AssetsFairValueDisclosure_d309c150-4bc5-4947-aed8-1d3fffc09fb0" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_35dd11e4-3beb-4c24-8b74-1a69bc3bc218" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_8ac0d230-57db-4eab-b4a1-abd482369dac" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_35dd11e4-3beb-4c24-8b74-1a69bc3bc218" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementBasisAxis_b67519f0-b653-4d12-9713-504777d664aa" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueByMeasurementBasisAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_35dd11e4-3beb-4c24-8b74-1a69bc3bc218" xlink:to="loc_us-gaap_FairValueByMeasurementBasisAxis_b67519f0-b653-4d12-9713-504777d664aa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_b67519f0-b653-4d12-9713-504777d664aa_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_b67519f0-b653-4d12-9713-504777d664aa" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_b67519f0-b653-4d12-9713-504777d664aa_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueDisclosureItemAmountsDomain_d7091043-71ea-4fdf-8e94-5484c81c375d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueDisclosureItemAmountsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_b67519f0-b653-4d12-9713-504777d664aa" xlink:to="loc_us-gaap_FairValueDisclosureItemAmountsDomain_d7091043-71ea-4fdf-8e94-5484c81c375d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_91cd40d7-03f6-4dd2-b490-676e81a42605" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueDisclosureItemAmountsDomain_d7091043-71ea-4fdf-8e94-5484c81c375d" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_91cd40d7-03f6-4dd2-b490-676e81a42605" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EstimateOfFairValueFairValueDisclosureMember_789fdee8-1e04-4c19-837a-4ff8669f2e8f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EstimateOfFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueDisclosureItemAmountsDomain_d7091043-71ea-4fdf-8e94-5484c81c375d" xlink:to="loc_us-gaap_EstimateOfFairValueFairValueDisclosureMember_789fdee8-1e04-4c19-837a-4ff8669f2e8f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementFrequencyAxis_4453d280-c792-41ca-9416-ba30ce41c825" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueByMeasurementFrequencyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_35dd11e4-3beb-4c24-8b74-1a69bc3bc218" xlink:to="loc_us-gaap_FairValueByMeasurementFrequencyAxis_4453d280-c792-41ca-9416-ba30ce41c825" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_4453d280-c792-41ca-9416-ba30ce41c825_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_4453d280-c792-41ca-9416-ba30ce41c825" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_4453d280-c792-41ca-9416-ba30ce41c825_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_66385507-3587-442b-b1da-a75ac9c0c21b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_4453d280-c792-41ca-9416-ba30ce41c825" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_66385507-3587-442b-b1da-a75ac9c0c21b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsNonrecurringMember_be5f398b-2893-41d8-86b5-9d3642933849" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueMeasurementsNonrecurringMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementFrequencyDomain_66385507-3587-442b-b1da-a75ac9c0c21b" xlink:to="loc_us-gaap_FairValueMeasurementsNonrecurringMember_be5f398b-2893-41d8-86b5-9d3642933849" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_8be7f7bb-73b4-41fa-9e3d-45c7dd9387ce" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_35dd11e4-3beb-4c24-8b74-1a69bc3bc218" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_8be7f7bb-73b4-41fa-9e3d-45c7dd9387ce" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_8be7f7bb-73b4-41fa-9e3d-45c7dd9387ce_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_8be7f7bb-73b4-41fa-9e3d-45c7dd9387ce" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_8be7f7bb-73b4-41fa-9e3d-45c7dd9387ce_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_d42fe406-9b9e-4d47-bb18-9b7b5178a899" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_8be7f7bb-73b4-41fa-9e3d-45c7dd9387ce" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_d42fe406-9b9e-4d47-bb18-9b7b5178a899" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_302b95bc-5314-44d5-aa8f-1ebb1c9d006e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_d42fe406-9b9e-4d47-bb18-9b7b5178a899" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_302b95bc-5314-44d5-aa8f-1ebb1c9d006e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_9067a6bd-2917-4cd3-8335-7b72118ed28e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_d42fe406-9b9e-4d47-bb18-9b7b5178a899" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_9067a6bd-2917-4cd3-8335-7b72118ed28e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_0011061c-dc0a-45fa-8eb4-91aae4ebf801" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueInputsLevel3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_d42fe406-9b9e-4d47-bb18-9b7b5178a899" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_0011061c-dc0a-45fa-8eb4-91aae4ebf801" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByAssetClassAxis_68e4074d-ea7a-475a-a6d0-e9d8d0aef88d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueByAssetClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_35dd11e4-3beb-4c24-8b74-1a69bc3bc218" xlink:to="loc_us-gaap_FairValueByAssetClassAxis_68e4074d-ea7a-475a-a6d0-e9d8d0aef88d" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_68e4074d-ea7a-475a-a6d0-e9d8d0aef88d_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByAssetClassAxis_68e4074d-ea7a-475a-a6d0-e9d8d0aef88d" xlink:to="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_68e4074d-ea7a-475a-a6d0-e9d8d0aef88d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_86835fd3-ed55-4803-a1f9-d1410bbbccd9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByAssetClassAxis_68e4074d-ea7a-475a-a6d0-e9d8d0aef88d" xlink:to="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_86835fd3-ed55-4803-a1f9-d1410bbbccd9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_ImpairedLoansMember_3471f613-d466-4041-bba8-8de85b61e179" xlink:href="efsc-20221231.xsd#efsc_ImpairedLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_86835fd3-ed55-4803-a1f9-d1410bbbccd9" xlink:to="loc_efsc_ImpairedLoansMember_3471f613-d466-4041-bba8-8de85b61e179" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_OtherRealEstateMember_12240a9f-9c1d-4a02-bb75-364470bf77ea" xlink:href="efsc-20221231.xsd#efsc_OtherRealEstateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_86835fd3-ed55-4803-a1f9-d1410bbbccd9" xlink:to="loc_efsc_OtherRealEstateMember_12240a9f-9c1d-4a02-bb75-364470bf77ea" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoanServicingAssetMember_b14f154d-dc9a-4d23-bb42-257bb017cc74" xlink:href="efsc-20221231.xsd#efsc_LoanServicingAssetMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_86835fd3-ed55-4803-a1f9-d1410bbbccd9" xlink:to="loc_efsc_LoanServicingAssetMember_b14f154d-dc9a-4d23-bb42-257bb017cc74" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/FairValueMeasurementsSummaryofCarryingAmountandFairValuesofFinancialInstrumentsReportedontheBalanceSheetsDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#FairValueMeasurementsSummaryofCarryingAmountandFairValuesofFinancialInstrumentsReportedontheBalanceSheetsDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/FairValueMeasurementsSummaryofCarryingAmountandFairValuesofFinancialInstrumentsReportedontheBalanceSheetsDetails" xlink:type="extended" id="ifcacdde761034ecd85432e9a9dfd6b13_FairValueMeasurementsSummaryofCarryingAmountandFairValuesofFinancialInstrumentsReportedontheBalanceSheetsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems_57799794-ed68-4a79-a6df-a9d20440d756" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_da19bdab-01a2-4b60-8f56-d129fa010a44" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems_57799794-ed68-4a79-a6df-a9d20440d756" xlink:to="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_da19bdab-01a2-4b60-8f56-d129fa010a44" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecurities_79a339e5-80fc-41f5-9cbc-f4694d825805" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_HeldToMaturitySecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_da19bdab-01a2-4b60-8f56-d129fa010a44" xlink:to="loc_us-gaap_HeldToMaturitySecurities_79a339e5-80fc-41f5-9cbc-f4694d825805" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentsFairValueDisclosure_8abd6a2f-d5e8-47ed-baad-2c599bdc694b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InvestmentsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_da19bdab-01a2-4b60-8f56-d129fa010a44" xlink:to="loc_us-gaap_InvestmentsFairValueDisclosure_8abd6a2f-d5e8-47ed-baad-2c599bdc694b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MortgagesHeldForSaleFairValueDisclosure_ecf388d1-ef41-473d-a04f-c6d9c1bf382a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_MortgagesHeldForSaleFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_da19bdab-01a2-4b60-8f56-d129fa010a44" xlink:to="loc_us-gaap_MortgagesHeldForSaleFairValueDisclosure_ecf388d1-ef41-473d-a04f-c6d9c1bf382a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoansAndLeasesReceivableNetReportedAmountFairValueDisclosure_0756ffdf-304f-453e-9a82-be2a2a40f74f" xlink:href="efsc-20221231.xsd#efsc_LoansAndLeasesReceivableNetReportedAmountFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_da19bdab-01a2-4b60-8f56-d129fa010a44" xlink:to="loc_efsc_LoansAndLeasesReceivableNetReportedAmountFairValueDisclosure_0756ffdf-304f-453e-9a82-be2a2a40f74f" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_StateTaxCreditsHeldForSaleFairValueDisclosure_3f672df3-e5e1-4158-9b5b-8d445e069dcb" xlink:href="efsc-20221231.xsd#efsc_StateTaxCreditsHeldForSaleFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_da19bdab-01a2-4b60-8f56-d129fa010a44" xlink:to="loc_efsc_StateTaxCreditsHeldForSaleFairValueDisclosure_3f672df3-e5e1-4158-9b5b-8d445e069dcb" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_InterestReceivableFairValueDisclosure_df59acc7-1bb8-4d81-902f-b2fdba375d05" xlink:href="efsc-20221231.xsd#efsc_InterestReceivableFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_da19bdab-01a2-4b60-8f56-d129fa010a44" xlink:to="loc_efsc_InterestReceivableFairValueDisclosure_df59acc7-1bb8-4d81-902f-b2fdba375d05" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_4a11564c-03b5-4d28-8b83-6f921114005a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems_57799794-ed68-4a79-a6df-a9d20440d756" xlink:to="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_4a11564c-03b5-4d28-8b83-6f921114005a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepositsFairValueDisclosure_a71faf4c-edf7-46a3-ac99-8231fcf5acb5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DepositsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_4a11564c-03b5-4d28-8b83-6f921114005a" xlink:to="loc_us-gaap_DepositsFairValueDisclosure_a71faf4c-edf7-46a3-ac99-8231fcf5acb5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueLiabilitiesMeasuredOnRecurringBasisSubordinatedDebtObligations_b145b47e-b7bb-4a33-a36a-57a9a4edc9a5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueLiabilitiesMeasuredOnRecurringBasisSubordinatedDebtObligations"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_4a11564c-03b5-4d28-8b83-6f921114005a" xlink:to="loc_us-gaap_FairValueLiabilitiesMeasuredOnRecurringBasisSubordinatedDebtObligations_b145b47e-b7bb-4a33-a36a-57a9a4edc9a5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FederalHomeLoanBankBorrowingsFairValueDisclosure_7fb74392-fb03-4eb0-80cd-e80ce322a237" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FederalHomeLoanBankBorrowingsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_4a11564c-03b5-4d28-8b83-6f921114005a" xlink:to="loc_us-gaap_FederalHomeLoanBankBorrowingsFairValueDisclosure_7fb74392-fb03-4eb0-80cd-e80ce322a237" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_OtherBorrowingsFairValueDisclosure_6b85b471-b030-4a66-b2f0-3442cbaa2084" xlink:href="efsc-20221231.xsd#efsc_OtherBorrowingsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_4a11564c-03b5-4d28-8b83-6f921114005a" xlink:to="loc_efsc_OtherBorrowingsFairValueDisclosure_6b85b471-b030-4a66-b2f0-3442cbaa2084" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByBalanceSheetGroupingTable_b8d8b641-f765-452c-8d58-16c7176fe333" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueByBalanceSheetGroupingTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems_57799794-ed68-4a79-a6df-a9d20440d756" xlink:to="loc_us-gaap_FairValueByBalanceSheetGroupingTable_b8d8b641-f765-452c-8d58-16c7176fe333" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementBasisAxis_b268bb92-6196-4fcb-85e3-21df21d9cfaf" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueByMeasurementBasisAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueByBalanceSheetGroupingTable_b8d8b641-f765-452c-8d58-16c7176fe333" xlink:to="loc_us-gaap_FairValueByMeasurementBasisAxis_b268bb92-6196-4fcb-85e3-21df21d9cfaf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_b268bb92-6196-4fcb-85e3-21df21d9cfaf_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_b268bb92-6196-4fcb-85e3-21df21d9cfaf" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_b268bb92-6196-4fcb-85e3-21df21d9cfaf_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueDisclosureItemAmountsDomain_3ae9621a-8a3f-4bfb-b17b-9e50851d15c3" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FairValueDisclosureItemAmountsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_b268bb92-6196-4fcb-85e3-21df21d9cfaf" xlink:to="loc_us-gaap_FairValueDisclosureItemAmountsDomain_3ae9621a-8a3f-4bfb-b17b-9e50851d15c3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_b832ec96-2357-4f77-8ba1-cf6fd8256044" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueDisclosureItemAmountsDomain_3ae9621a-8a3f-4bfb-b17b-9e50851d15c3" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_b832ec96-2357-4f77-8ba1-cf6fd8256044" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CarryingReportedAmountFairValueDisclosureMember_c8de7dbc-21e4-4ede-b191-7743565fa50e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CarryingReportedAmountFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueDisclosureItemAmountsDomain_3ae9621a-8a3f-4bfb-b17b-9e50851d15c3" xlink:to="loc_us-gaap_CarryingReportedAmountFairValueDisclosureMember_c8de7dbc-21e4-4ede-b191-7743565fa50e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EstimateOfFairValueFairValueDisclosureMember_8fc2015e-6f9b-4359-bad3-313e85efd2a9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_EstimateOfFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueDisclosureItemAmountsDomain_3ae9621a-8a3f-4bfb-b17b-9e50851d15c3" xlink:to="loc_us-gaap_EstimateOfFairValueFairValueDisclosureMember_8fc2015e-6f9b-4359-bad3-313e85efd2a9" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/ParentCompanyOnlyCondensedFinancialStatementsCondensedBalanceSheetsDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#ParentCompanyOnlyCondensedFinancialStatementsCondensedBalanceSheetsDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/ParentCompanyOnlyCondensedFinancialStatementsCondensedBalanceSheetsDetails" xlink:type="extended" id="i0f39ab8509464783896bd2c3054ffbd1_ParentCompanyOnlyCondensedFinancialStatementsCondensedBalanceSheetsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_srt_CondensedFinancialStatementsCaptionsLineItems_107a5797-535d-4257-9683-5e3fd6cc482f" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_CondensedFinancialStatementsCaptionsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_ebde4b84-4524-492d-ab1c-77ff8b90573b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_CondensedFinancialStatementsCaptionsLineItems_107a5797-535d-4257-9683-5e3fd6cc482f" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_ebde4b84-4524-492d-ab1c-77ff8b90573b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentsInAffiliatesSubsidiariesAssociatesAndJointVentures_614a85d1-0f21-4f9b-aca7-7fa9ec918cc2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InvestmentsInAffiliatesSubsidiariesAssociatesAndJointVentures"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_CondensedFinancialStatementsCaptionsLineItems_107a5797-535d-4257-9683-5e3fd6cc482f" xlink:to="loc_us-gaap_InvestmentsInAffiliatesSubsidiariesAssociatesAndJointVentures_614a85d1-0f21-4f9b-aca7-7fa9ec918cc2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssets_2cb58d61-8b92-4b2b-a2ac-87eec3afee62" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_CondensedFinancialStatementsCaptionsLineItems_107a5797-535d-4257-9683-5e3fd6cc482f" xlink:to="loc_us-gaap_OtherAssets_2cb58d61-8b92-4b2b-a2ac-87eec3afee62" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Assets_95be6166-64bc-436b-b329-9516eed18a43" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_Assets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_CondensedFinancialStatementsCaptionsLineItems_107a5797-535d-4257-9683-5e3fd6cc482f" xlink:to="loc_us-gaap_Assets_95be6166-64bc-436b-b329-9516eed18a43" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubordinatedDebt_e257a373-d8fb-498b-8fb4-2c6b2e6c4fbc" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SubordinatedDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_CondensedFinancialStatementsCaptionsLineItems_107a5797-535d-4257-9683-5e3fd6cc482f" xlink:to="loc_us-gaap_SubordinatedDebt_e257a373-d8fb-498b-8fb4-2c6b2e6c4fbc" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NotesPayableToBank_e395d6d9-20e5-4dc0-963d-ca12193ac4ec" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NotesPayableToBank"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_CondensedFinancialStatementsCaptionsLineItems_107a5797-535d-4257-9683-5e3fd6cc482f" xlink:to="loc_us-gaap_NotesPayableToBank_e395d6d9-20e5-4dc0-963d-ca12193ac4ec" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsPayableAndAccruedLiabilitiesCurrentAndNoncurrent_9f1b0893-83e9-4360-bcd8-0fd2231cab34" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AccountsPayableAndAccruedLiabilitiesCurrentAndNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_CondensedFinancialStatementsCaptionsLineItems_107a5797-535d-4257-9683-5e3fd6cc482f" xlink:to="loc_us-gaap_AccountsPayableAndAccruedLiabilitiesCurrentAndNoncurrent_9f1b0893-83e9-4360-bcd8-0fd2231cab34" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_d3308bbc-75a5-4d08-8b15-5a8841866840" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_CondensedFinancialStatementsCaptionsLineItems_107a5797-535d-4257-9683-5e3fd6cc482f" xlink:to="loc_us-gaap_StockholdersEquity_d3308bbc-75a5-4d08-8b15-5a8841866840" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquity_81d1faef-777a-4d2f-9f80-4f663bfcaca5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_LiabilitiesAndStockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_CondensedFinancialStatementsCaptionsLineItems_107a5797-535d-4257-9683-5e3fd6cc482f" xlink:to="loc_us-gaap_LiabilitiesAndStockholdersEquity_81d1faef-777a-4d2f-9f80-4f663bfcaca5" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScheduleOfCondensedFinancialStatementsTable_f15fb578-7587-44d1-bad7-c87031b3fe58" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ScheduleOfCondensedFinancialStatementsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_srt_CondensedFinancialStatementsCaptionsLineItems_107a5797-535d-4257-9683-5e3fd6cc482f" xlink:to="loc_srt_ScheduleOfCondensedFinancialStatementsTable_f15fb578-7587-44d1-bad7-c87031b3fe58" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentTypeAxis_7b3f4f66-a991-47f0-b26e-3bba3f11c834" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InvestmentTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_srt_ScheduleOfCondensedFinancialStatementsTable_f15fb578-7587-44d1-bad7-c87031b3fe58" xlink:to="loc_us-gaap_InvestmentTypeAxis_7b3f4f66-a991-47f0-b26e-3bba3f11c834" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentTypeCategorizationMember_7b3f4f66-a991-47f0-b26e-3bba3f11c834_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InvestmentTypeCategorizationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_InvestmentTypeAxis_7b3f4f66-a991-47f0-b26e-3bba3f11c834" xlink:to="loc_us-gaap_InvestmentTypeCategorizationMember_7b3f4f66-a991-47f0-b26e-3bba3f11c834_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentTypeCategorizationMember_5aae2da9-19b2-46d5-972e-1c78196ddb78" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InvestmentTypeCategorizationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_InvestmentTypeAxis_7b3f4f66-a991-47f0-b26e-3bba3f11c834" xlink:to="loc_us-gaap_InvestmentTypeCategorizationMember_5aae2da9-19b2-46d5-972e-1c78196ddb78" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_EnterpriseBankAndTrustMember_e340d51e-f53d-4979-b2cf-24dd6267cd8a" xlink:href="efsc-20221231.xsd#efsc_EnterpriseBankAndTrustMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InvestmentTypeCategorizationMember_5aae2da9-19b2-46d5-972e-1c78196ddb78" xlink:to="loc_efsc_EnterpriseBankAndTrustMember_e340d51e-f53d-4979-b2cf-24dd6267cd8a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_EnterpriseFinancialCdeLlcMember_bd1d51f1-190c-4b30-8731-1951e54cfb1e" xlink:href="efsc-20221231.xsd#efsc_EnterpriseFinancialCdeLlcMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InvestmentTypeCategorizationMember_5aae2da9-19b2-46d5-972e-1c78196ddb78" xlink:to="loc_efsc_EnterpriseFinancialCdeLlcMember_bd1d51f1-190c-4b30-8731-1951e54cfb1e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidatedEntitiesAxis_950f8ed9-f2e9-4006-a7ed-521fd7c2e31f" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ConsolidatedEntitiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_srt_ScheduleOfCondensedFinancialStatementsTable_f15fb578-7587-44d1-bad7-c87031b3fe58" xlink:to="loc_srt_ConsolidatedEntitiesAxis_950f8ed9-f2e9-4006-a7ed-521fd7c2e31f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidatedEntitiesDomain_950f8ed9-f2e9-4006-a7ed-521fd7c2e31f_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ConsolidatedEntitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ConsolidatedEntitiesAxis_950f8ed9-f2e9-4006-a7ed-521fd7c2e31f" xlink:to="loc_srt_ConsolidatedEntitiesDomain_950f8ed9-f2e9-4006-a7ed-521fd7c2e31f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidatedEntitiesDomain_fa5ebf30-249a-4d59-bd0f-7f29d525719b" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ConsolidatedEntitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ConsolidatedEntitiesAxis_950f8ed9-f2e9-4006-a7ed-521fd7c2e31f" xlink:to="loc_srt_ConsolidatedEntitiesDomain_fa5ebf30-249a-4d59-bd0f-7f29d525719b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ParentCompanyMember_13a6d3ff-c7e8-4426-ba30-5211e6bc6d53" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ParentCompanyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidatedEntitiesDomain_fa5ebf30-249a-4d59-bd0f-7f29d525719b" xlink:to="loc_srt_ParentCompanyMember_13a6d3ff-c7e8-4426-ba30-5211e6bc6d53" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/ParentCompanyOnlyCondensedFinancialStatementsCondensedStatementsofOperationsDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#ParentCompanyOnlyCondensedFinancialStatementsCondensedStatementsofOperationsDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/ParentCompanyOnlyCondensedFinancialStatementsCondensedStatementsofOperationsDetails" xlink:type="extended" id="idc2fe8649d1748269678e0b8bbf094ca_ParentCompanyOnlyCondensedFinancialStatementsCondensedStatementsofOperationsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_ebbf6064-3621-4c5f-987c-59fec2a47c0d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLossAbstract_b7edec04-cdc7-4465-a68f-236779d66bb9" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OperatingIncomeLossAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_ebbf6064-3621-4c5f-987c-59fec2a47c0d" xlink:to="loc_us-gaap_OperatingIncomeLossAbstract_b7edec04-cdc7-4465-a68f-236779d66bb9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DividendIncomeOperating_516e3a2c-b221-4d2e-a3f8-1da3112c5b0d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_DividendIncomeOperating"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingIncomeLossAbstract_b7edec04-cdc7-4465-a68f-236779d66bb9" xlink:to="loc_us-gaap_DividendIncomeOperating_516e3a2c-b221-4d2e-a3f8-1da3112c5b0d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_DividendsfromOtherSubsidiaries_fc0b46aa-1cfd-42eb-a5e5-957ea81f9a29" xlink:href="efsc-20221231.xsd#efsc_DividendsfromOtherSubsidiaries"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingIncomeLossAbstract_b7edec04-cdc7-4465-a68f-236779d66bb9" xlink:to="loc_efsc_DividendsfromOtherSubsidiaries_fc0b46aa-1cfd-42eb-a5e5-957ea81f9a29" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherIncome_3dea87e0-1831-4cec-a45c-cb6bb7d75a97" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingIncomeLossAbstract_b7edec04-cdc7-4465-a68f-236779d66bb9" xlink:to="loc_us-gaap_OtherIncome_3dea87e0-1831-4cec-a45c-cb6bb7d75a97" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Revenues_de390d4d-2553-442d-bc42-df7138f01764" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_Revenues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingIncomeLossAbstract_b7edec04-cdc7-4465-a68f-236779d66bb9" xlink:to="loc_us-gaap_Revenues_de390d4d-2553-442d-bc42-df7138f01764" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingExpensesAbstract_e8de8700-f8e6-4e6c-a6c5-18fbbf531d1a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OperatingExpensesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_ebbf6064-3621-4c5f-987c-59fec2a47c0d" xlink:to="loc_us-gaap_OperatingExpensesAbstract_e8de8700-f8e6-4e6c-a6c5-18fbbf531d1a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseSubordinatedNotesAndDebentures_5f2322de-32a6-4d66-a418-275bf89a52a0" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterestExpenseSubordinatedNotesAndDebentures"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_e8de8700-f8e6-4e6c-a6c5-18fbbf531d1a" xlink:to="loc_us-gaap_InterestExpenseSubordinatedNotesAndDebentures_5f2322de-32a6-4d66-a418-275bf89a52a0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherExpenses_b16dfbfb-663e-4e8d-8ea2-3ee622876892" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherExpenses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_e8de8700-f8e6-4e6c-a6c5-18fbbf531d1a" xlink:to="loc_us-gaap_OtherExpenses_b16dfbfb-663e-4e8d-8ea2-3ee622876892" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingExpenses_8f1b632f-9af1-4f98-9836-ae88617151cb" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OperatingExpenses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_e8de8700-f8e6-4e6c-a6c5-18fbbf531d1a" xlink:to="loc_us-gaap_OperatingExpenses_8f1b632f-9af1-4f98-9836-ae88617151cb" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperations_65645033-9b69-45bd-9e9a-cf93f60e933a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeLossFromContinuingOperations"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_ebbf6064-3621-4c5f-987c-59fec2a47c0d" xlink:to="loc_us-gaap_IncomeLossFromContinuingOperations_65645033-9b69-45bd-9e9a-cf93f60e933a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_80c560b4-fdee-4a43-aae8-fe3136eb13d6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeTaxExpenseBenefit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_ebbf6064-3621-4c5f-987c-59fec2a47c0d" xlink:to="loc_us-gaap_IncomeTaxExpenseBenefit_80c560b4-fdee-4a43-aae8-fe3136eb13d6" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_997d364c-f860-4604-bb67-43db2acc7d87" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_ebbf6064-3621-4c5f-987c-59fec2a47c0d" xlink:to="loc_us-gaap_NetIncomeLoss_997d364c-f860-4604-bb67-43db2acc7d87" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UndistributedEarnings_7ea7ba1e-ad46-4f3b-8e76-2f7ab9b02d0a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_UndistributedEarnings"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_ebbf6064-3621-4c5f-987c-59fec2a47c0d" xlink:to="loc_us-gaap_UndistributedEarnings_7ea7ba1e-ad46-4f3b-8e76-2f7ab9b02d0a" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ComprehensiveIncomeNetOfTax_d66c3bcc-192c-4dde-a691-4f5e2ea84ec1" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ComprehensiveIncomeNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_ebbf6064-3621-4c5f-987c-59fec2a47c0d" xlink:to="loc_us-gaap_ComprehensiveIncomeNetOfTax_d66c3bcc-192c-4dde-a691-4f5e2ea84ec1" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScheduleOfCondensedFinancialStatementsTable_cac5d066-b52b-426f-9857-cdc0333d9b1f" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ScheduleOfCondensedFinancialStatementsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_ebbf6064-3621-4c5f-987c-59fec2a47c0d" xlink:to="loc_srt_ScheduleOfCondensedFinancialStatementsTable_cac5d066-b52b-426f-9857-cdc0333d9b1f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidatedEntitiesAxis_36a36c02-413f-4976-99f0-90c7b3cb0aed" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ConsolidatedEntitiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_srt_ScheduleOfCondensedFinancialStatementsTable_cac5d066-b52b-426f-9857-cdc0333d9b1f" xlink:to="loc_srt_ConsolidatedEntitiesAxis_36a36c02-413f-4976-99f0-90c7b3cb0aed" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidatedEntitiesDomain_36a36c02-413f-4976-99f0-90c7b3cb0aed_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ConsolidatedEntitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ConsolidatedEntitiesAxis_36a36c02-413f-4976-99f0-90c7b3cb0aed" xlink:to="loc_srt_ConsolidatedEntitiesDomain_36a36c02-413f-4976-99f0-90c7b3cb0aed_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidatedEntitiesDomain_cd6801ed-06b9-4890-a225-915844edd6c9" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ConsolidatedEntitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ConsolidatedEntitiesAxis_36a36c02-413f-4976-99f0-90c7b3cb0aed" xlink:to="loc_srt_ConsolidatedEntitiesDomain_cd6801ed-06b9-4890-a225-915844edd6c9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ParentCompanyMember_6950f3b8-0e8d-4f88-8111-d38ea97713af" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ParentCompanyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidatedEntitiesDomain_cd6801ed-06b9-4890-a225-915844edd6c9" xlink:to="loc_srt_ParentCompanyMember_6950f3b8-0e8d-4f88-8111-d38ea97713af" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/ParentCompanyOnlyCondensedFinancialStatementsConsolidatedStatementsofCashFlowDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#ParentCompanyOnlyCondensedFinancialStatementsConsolidatedStatementsofCashFlowDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/ParentCompanyOnlyCondensedFinancialStatementsConsolidatedStatementsofCashFlowDetails" xlink:type="extended" id="ia362c24aa2b64f9b96c7b2c69d545484_ParentCompanyOnlyCondensedFinancialStatementsConsolidatedStatementsofCashFlowDetails">
    <link:loc xlink:type="locator" xlink:label="loc_srt_CondensedFinancialStatementsCaptionsLineItems_0fdb7f19-c876-4412-a22f-3acfbe6d3d80" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_CondensedFinancialStatementsCaptionsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_ee81a05d-9407-43f4-92a9-496e51f58e28" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_CondensedFinancialStatementsCaptionsLineItems_0fdb7f19-c876-4412-a22f-3acfbe6d3d80" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_ee81a05d-9407-43f4-92a9-496e51f58e28" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic_7e2f132c-0309-471d-a9cf-4568bf5683ae" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_ee81a05d-9407-43f4-92a9-496e51f58e28" xlink:to="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic_7e2f132c-0309-471d-a9cf-4568bf5683ae" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_52054e59-a3b0-470d-9e7e-47b6aac5b2b8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_ee81a05d-9407-43f4-92a9-496e51f58e28" xlink:to="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_52054e59-a3b0-470d-9e7e-47b6aac5b2b8" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensation_f5cd83f0-b6db-4bb2-82dc-e9fcfba4b392" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_52054e59-a3b0-470d-9e7e-47b6aac5b2b8" xlink:to="loc_us-gaap_ShareBasedCompensation_f5cd83f0-b6db-4bb2-82dc-e9fcfba4b392" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromEquityMethodInvestments_67d32e2f-fb2d-415b-9b8d-6e7889aeede4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_IncomeLossFromEquityMethodInvestments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_52054e59-a3b0-470d-9e7e-47b6aac5b2b8" xlink:to="loc_us-gaap_IncomeLossFromEquityMethodInvestments_67d32e2f-fb2d-415b-9b8d-6e7889aeede4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromDividendsReceived_50bcbcc1-302e-48be-a724-9d0d321c63bd" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ProceedsFromDividendsReceived"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_52054e59-a3b0-470d-9e7e-47b6aac5b2b8" xlink:to="loc_us-gaap_ProceedsFromDividendsReceived_50bcbcc1-302e-48be-a724-9d0d321c63bd" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherOperatingActivitiesCashFlowStatement_2f53844b-52dd-4d19-b3f3-406009cc9eed" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherOperatingActivitiesCashFlowStatement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_52054e59-a3b0-470d-9e7e-47b6aac5b2b8" xlink:to="loc_us-gaap_OtherOperatingActivitiesCashFlowStatement_2f53844b-52dd-4d19-b3f3-406009cc9eed" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_41ad8620-d62d-4f1a-b2a5-0459897b7324" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_52054e59-a3b0-470d-9e7e-47b6aac5b2b8" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_41ad8620-d62d-4f1a-b2a5-0459897b7324" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_c1de23f8-0268-4092-b7f5-9a3749bdd0df" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_CondensedFinancialStatementsCaptionsLineItems_0fdb7f19-c876-4412-a22f-3acfbe6d3d80" xlink:to="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_c1de23f8-0268-4092-b7f5-9a3749bdd0df" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireBusinessesNetOfCashAcquired_9b34c13a-fdc0-4ef4-a8b7-10b89e199aa8" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PaymentsToAcquireBusinessesNetOfCashAcquired"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_c1de23f8-0268-4092-b7f5-9a3749bdd0df" xlink:to="loc_us-gaap_PaymentsToAcquireBusinessesNetOfCashAcquired_9b34c13a-fdc0-4ef4-a8b7-10b89e199aa8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireInvestments_301b7658-3a15-4067-894d-5640d6857185" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PaymentsToAcquireInvestments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_c1de23f8-0268-4092-b7f5-9a3749bdd0df" xlink:to="loc_us-gaap_PaymentsToAcquireInvestments_301b7658-3a15-4067-894d-5640d6857185" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromInterestAndDividendsReceived_a1c912d6-fdee-4061-9efb-7e2bd9cbd87c" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ProceedsFromInterestAndDividendsReceived"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_c1de23f8-0268-4092-b7f5-9a3749bdd0df" xlink:to="loc_us-gaap_ProceedsFromInterestAndDividendsReceived_a1c912d6-fdee-4061-9efb-7e2bd9cbd87c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_b7839229-de1a-4d08-bc40-edeacafa1387" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivitiesAbstract_c1de23f8-0268-4092-b7f5-9a3749bdd0df" xlink:to="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_b7839229-de1a-4d08-bc40-edeacafa1387" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_ff612840-c30a-4a0e-abb0-726743d12b64" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_CondensedFinancialStatementsCaptionsLineItems_0fdb7f19-c876-4412-a22f-3acfbe6d3d80" xlink:to="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_ff612840-c30a-4a0e-abb0-726743d12b64" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOfSubordinatedLongTermDebt_09e0a9e8-5d36-401d-bff1-8be6fadff4d6" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ProceedsFromIssuanceOfSubordinatedLongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_ff612840-c30a-4a0e-abb0-726743d12b64" xlink:to="loc_us-gaap_ProceedsFromIssuanceOfSubordinatedLongTermDebt_09e0a9e8-5d36-401d-bff1-8be6fadff4d6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RepaymentsOfSubordinatedDebt_90d25bb5-4161-4a71-a350-db9a78d5f77a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RepaymentsOfSubordinatedDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_ff612840-c30a-4a0e-abb0-726743d12b64" xlink:to="loc_us-gaap_RepaymentsOfSubordinatedDebt_90d25bb5-4161-4a71-a350-db9a78d5f77a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RepaymentsOfNotesPayable_12545f70-12c8-4071-af10-7bea2d6a0284" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RepaymentsOfNotesPayable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_ff612840-c30a-4a0e-abb0-726743d12b64" xlink:to="loc_us-gaap_RepaymentsOfNotesPayable_12545f70-12c8-4071-af10-7bea2d6a0284" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsOfDividendsCommonStock_dca60ba4-1140-4a3f-bb6f-f2d3b12f17da" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PaymentsOfDividendsCommonStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_ff612840-c30a-4a0e-abb0-726743d12b64" xlink:to="loc_us-gaap_PaymentsOfDividendsCommonStock_dca60ba4-1140-4a3f-bb6f-f2d3b12f17da" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsForRepurchaseOfCommonStock_9dafa1cf-13fd-4206-801c-bd1ee544f3ee" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PaymentsForRepurchaseOfCommonStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_ff612840-c30a-4a0e-abb0-726743d12b64" xlink:to="loc_us-gaap_PaymentsForRepurchaseOfCommonStock_9dafa1cf-13fd-4206-801c-bd1ee544f3ee" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOfPreferredStockAndPreferenceStock_e5964f4a-f55a-4870-bc40-0def7fccede5" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ProceedsFromIssuanceOfPreferredStockAndPreferenceStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_ff612840-c30a-4a0e-abb0-726743d12b64" xlink:to="loc_us-gaap_ProceedsFromIssuanceOfPreferredStockAndPreferenceStock_e5964f4a-f55a-4870-bc40-0def7fccede5" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsOfDividendsPreferredStockAndPreferenceStock_cbe3eacf-70dd-475a-9117-db076a4de10f" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_PaymentsOfDividendsPreferredStockAndPreferenceStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_ff612840-c30a-4a0e-abb0-726743d12b64" xlink:to="loc_us-gaap_PaymentsOfDividendsPreferredStockAndPreferenceStock_cbe3eacf-70dd-475a-9117-db076a4de10f" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOfSharesUnderIncentiveAndShareBasedCompensationPlansIncludingStockOptions_37a4b740-5b1b-4f29-83a0-a95a79e3566e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_ProceedsFromIssuanceOfSharesUnderIncentiveAndShareBasedCompensationPlansIncludingStockOptions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_ff612840-c30a-4a0e-abb0-726743d12b64" xlink:to="loc_us-gaap_ProceedsFromIssuanceOfSharesUnderIncentiveAndShareBasedCompensationPlansIncludingStockOptions_37a4b740-5b1b-4f29-83a0-a95a79e3566e" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_8692b919-4eb5-4a56-a153-d08f676a0bc4" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivitiesAbstract_ff612840-c30a-4a0e-abb0-726743d12b64" xlink:to="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_8692b919-4eb5-4a56-a153-d08f676a0bc4" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsPeriodIncreaseDecrease_ff1f4eb8-b88d-45ee-8ca1-9de8a7e76cfa" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CashAndCashEquivalentsPeriodIncreaseDecrease"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_CondensedFinancialStatementsCaptionsLineItems_0fdb7f19-c876-4412-a22f-3acfbe6d3d80" xlink:to="loc_us-gaap_CashAndCashEquivalentsPeriodIncreaseDecrease_ff1f4eb8-b88d-45ee-8ca1-9de8a7e76cfa" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_7b9b003c-c146-4fcc-8a83-d5bbabae98d2" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_CondensedFinancialStatementsCaptionsLineItems_0fdb7f19-c876-4412-a22f-3acfbe6d3d80" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_7b9b003c-c146-4fcc-8a83-d5bbabae98d2" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_635d137b-43c5-48fd-8e82-05b3eed17a58" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SupplementalCashFlowInformationAbstract_1ed4d439-eb99-4417-bb82-2db5ca56d570" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_SupplementalCashFlowInformationAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_CondensedFinancialStatementsCaptionsLineItems_0fdb7f19-c876-4412-a22f-3acfbe6d3d80" xlink:to="loc_us-gaap_SupplementalCashFlowInformationAbstract_1ed4d439-eb99-4417-bb82-2db5ca56d570" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NoncashInvestingAndFinancingItemsAbstract_d4f41069-f195-400b-b054-8e7c654eab1d" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NoncashInvestingAndFinancingItemsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_CondensedFinancialStatementsCaptionsLineItems_0fdb7f19-c876-4412-a22f-3acfbe6d3d80" xlink:to="loc_us-gaap_NoncashInvestingAndFinancingItemsAbstract_d4f41069-f195-400b-b054-8e7c654eab1d" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesAcquisitions_e876974e-3986-4e40-9c80-f69d57c11130" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_StockIssuedDuringPeriodSharesAcquisitions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_CondensedFinancialStatementsCaptionsLineItems_0fdb7f19-c876-4412-a22f-3acfbe6d3d80" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesAcquisitions_e876974e-3986-4e40-9c80-f69d57c11130" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RepaymentsOfLongTermDebt_37be3842-28c5-4682-9424-422307c40f6a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_RepaymentsOfLongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_CondensedFinancialStatementsCaptionsLineItems_0fdb7f19-c876-4412-a22f-3acfbe6d3d80" xlink:to="loc_us-gaap_RepaymentsOfLongTermDebt_37be3842-28c5-4682-9424-422307c40f6a" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScheduleOfCondensedFinancialStatementsTable_14c06c77-85f8-49b0-b6af-ab95a808f0a9" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ScheduleOfCondensedFinancialStatementsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_srt_CondensedFinancialStatementsCaptionsLineItems_0fdb7f19-c876-4412-a22f-3acfbe6d3d80" xlink:to="loc_srt_ScheduleOfCondensedFinancialStatementsTable_14c06c77-85f8-49b0-b6af-ab95a808f0a9" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidatedEntitiesAxis_b5dd8961-ef02-47a2-a83e-162e6b2131fa" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ConsolidatedEntitiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_srt_ScheduleOfCondensedFinancialStatementsTable_14c06c77-85f8-49b0-b6af-ab95a808f0a9" xlink:to="loc_srt_ConsolidatedEntitiesAxis_b5dd8961-ef02-47a2-a83e-162e6b2131fa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidatedEntitiesDomain_b5dd8961-ef02-47a2-a83e-162e6b2131fa_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ConsolidatedEntitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ConsolidatedEntitiesAxis_b5dd8961-ef02-47a2-a83e-162e6b2131fa" xlink:to="loc_srt_ConsolidatedEntitiesDomain_b5dd8961-ef02-47a2-a83e-162e6b2131fa_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidatedEntitiesDomain_fd735f82-3a5b-4639-b2bf-eb20f38a9f15" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ConsolidatedEntitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ConsolidatedEntitiesAxis_b5dd8961-ef02-47a2-a83e-162e6b2131fa" xlink:to="loc_srt_ConsolidatedEntitiesDomain_fd735f82-3a5b-4639-b2bf-eb20f38a9f15" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ParentCompanyMember_d6da72d4-ba73-42e0-9bc4-4bc17513e696" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ParentCompanyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidatedEntitiesDomain_fd735f82-3a5b-4639-b2bf-eb20f38a9f15" xlink:to="loc_srt_ParentCompanyMember_d6da72d4-ba73-42e0-9bc4-4bc17513e696" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/SupplementalFinancialInformationDetails" xlink:type="simple" xlink:href="efsc-20221231.xsd#SupplementalFinancialInformationDetails"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/SupplementalFinancialInformationDetails" xlink:type="extended" id="i798fdea0394247db9d90fe05805fd25f_SupplementalFinancialInformationDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterimPeriodCostsNotAllocableLineItems_5809dc8a-bf3c-4d91-a133-5425a3ec902e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterimPeriodCostsNotAllocableLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NoninterestIncome_838fc0d7-2fc6-4fed-8d52-0574b941ca8e" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NoninterestIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterimPeriodCostsNotAllocableLineItems_5809dc8a-bf3c-4d91-a133-5425a3ec902e" xlink:to="loc_us-gaap_NoninterestIncome_838fc0d7-2fc6-4fed-8d52-0574b941ca8e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNoninterestExpense_6e063940-2746-4049-ac2d-c98bfd4e851a" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_OtherNoninterestExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterimPeriodCostsNotAllocableLineItems_5809dc8a-bf3c-4d91-a133-5425a3ec902e" xlink:to="loc_us-gaap_OtherNoninterestExpense_6e063940-2746-4049-ac2d-c98bfd4e851a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterimPeriodCostsNotAllocableTable_fee5675a-0a19-4456-91b4-8f87f9bc9721" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterimPeriodCostsNotAllocableTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_InterimPeriodCostsNotAllocableLineItems_5809dc8a-bf3c-4d91-a133-5425a3ec902e" xlink:to="loc_us-gaap_InterimPeriodCostsNotAllocableTable_fee5675a-0a19-4456-91b4-8f87f9bc9721" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_bff5ca2f-733f-476c-9b04-1ffb97e6d4bd" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_InterimPeriodCostsNotAllocableTable_fee5675a-0a19-4456-91b4-8f87f9bc9721" xlink:to="loc_srt_ProductOrServiceAxis_bff5ca2f-733f-476c-9b04-1ffb97e6d4bd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_bff5ca2f-733f-476c-9b04-1ffb97e6d4bd_default" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_bff5ca2f-733f-476c-9b04-1ffb97e6d4bd" xlink:to="loc_srt_ProductsAndServicesDomain_bff5ca2f-733f-476c-9b04-1ffb97e6d4bd_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_a510054b-1c4c-4e60-b3cd-fe8cef35faa3" xlink:href="https://xbrl.fasb.org/srt/2021/elts/srt-2021-01-31.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_bff5ca2f-733f-476c-9b04-1ffb97e6d4bd" xlink:to="loc_srt_ProductsAndServicesDomain_a510054b-1c4c-4e60-b3cd-fe8cef35faa3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CommunityDevelopmentFeesMember_3588e679-3642-48e9-bb51-3ce308115fbf" xlink:href="efsc-20221231.xsd#efsc_CommunityDevelopmentFeesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_a510054b-1c4c-4e60-b3cd-fe8cef35faa3" xlink:to="loc_efsc_CommunityDevelopmentFeesMember_3588e679-3642-48e9-bb51-3ce308115fbf" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_BankOwnedLifeInsuranceMember_12db11e0-407a-47f3-827c-014828828a47" xlink:href="efsc-20221231.xsd#efsc_BankOwnedLifeInsuranceMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_a510054b-1c4c-4e60-b3cd-fe8cef35faa3" xlink:to="loc_efsc_BankOwnedLifeInsuranceMember_12db11e0-407a-47f3-827c-014828828a47" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_OtherMiscellaneousIncomeMember_b63dcb6f-01c3-4c5d-a4c3-05746c6f808f" xlink:href="efsc-20221231.xsd#efsc_OtherMiscellaneousIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_a510054b-1c4c-4e60-b3cd-fe8cef35faa3" xlink:to="loc_efsc_OtherMiscellaneousIncomeMember_b63dcb6f-01c3-4c5d-a4c3-05746c6f808f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialServiceOtherMember_8ae78ac2-0f54-4525-986a-40048475c500" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_FinancialServiceOtherMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_a510054b-1c4c-4e60-b3cd-fe8cef35faa3" xlink:to="loc_us-gaap_FinancialServiceOtherMember_8ae78ac2-0f54-4525-986a-40048475c500" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NatureOfExpenseAxis_c48957bc-ca31-4422-970c-4fc2c306668b" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_NatureOfExpenseAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_InterimPeriodCostsNotAllocableTable_fee5675a-0a19-4456-91b4-8f87f9bc9721" xlink:to="loc_us-gaap_NatureOfExpenseAxis_c48957bc-ca31-4422-970c-4fc2c306668b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterimPeriodCostsNotAllocableDomain_c48957bc-ca31-4422-970c-4fc2c306668b_default" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterimPeriodCostsNotAllocableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_NatureOfExpenseAxis_c48957bc-ca31-4422-970c-4fc2c306668b" xlink:to="loc_us-gaap_InterimPeriodCostsNotAllocableDomain_c48957bc-ca31-4422-970c-4fc2c306668b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterimPeriodCostsNotAllocableDomain_58f3ff12-ca6c-4e87-871e-da28d1a0ac03" xlink:href="https://xbrl.fasb.org/us-gaap/2021/elts/us-gaap-2021-01-31.xsd#us-gaap_InterimPeriodCostsNotAllocableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_NatureOfExpenseAxis_c48957bc-ca31-4422-970c-4fc2c306668b" xlink:to="loc_us-gaap_InterimPeriodCostsNotAllocableDomain_58f3ff12-ca6c-4e87-871e-da28d1a0ac03" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_AmortizationExpenseMember_082d9af4-a895-46e6-8e5c-71772d18d315" xlink:href="efsc-20221231.xsd#efsc_AmortizationExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterimPeriodCostsNotAllocableDomain_58f3ff12-ca6c-4e87-871e-da28d1a0ac03" xlink:to="loc_efsc_AmortizationExpenseMember_082d9af4-a895-46e6-8e5c-71772d18d315" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_BankingExpensesMember_76ac4e76-7a73-4202-9941-c70d70095401" xlink:href="efsc-20221231.xsd#efsc_BankingExpensesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterimPeriodCostsNotAllocableDomain_58f3ff12-ca6c-4e87-871e-da28d1a0ac03" xlink:to="loc_efsc_BankingExpensesMember_76ac4e76-7a73-4202-9941-c70d70095401" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_CustomerAnalysisExpenseMember_854ff200-8fe6-4e2d-9efe-455d87780dc0" xlink:href="efsc-20221231.xsd#efsc_CustomerAnalysisExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterimPeriodCostsNotAllocableDomain_58f3ff12-ca6c-4e87-871e-da28d1a0ac03" xlink:to="loc_efsc_CustomerAnalysisExpenseMember_854ff200-8fe6-4e2d-9efe-455d87780dc0" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_FDICAndOtherInsurnaceMember_4b675d0a-1887-4cf4-b9eb-a8b33650743d" xlink:href="efsc-20221231.xsd#efsc_FDICAndOtherInsurnaceMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterimPeriodCostsNotAllocableDomain_58f3ff12-ca6c-4e87-871e-da28d1a0ac03" xlink:to="loc_efsc_FDICAndOtherInsurnaceMember_4b675d0a-1887-4cf4-b9eb-a8b33650743d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_LoanLegalExpensesMember_5695e0b1-35a5-484a-aab5-e80ab528e402" xlink:href="efsc-20221231.xsd#efsc_LoanLegalExpensesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterimPeriodCostsNotAllocableDomain_58f3ff12-ca6c-4e87-871e-da28d1a0ac03" xlink:to="loc_efsc_LoanLegalExpensesMember_5695e0b1-35a5-484a-aab5-e80ab528e402" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_OutsideServicesMember_00b9af51-1c3c-40ce-89f5-d881b2fd4fe2" xlink:href="efsc-20221231.xsd#efsc_OutsideServicesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterimPeriodCostsNotAllocableDomain_58f3ff12-ca6c-4e87-871e-da28d1a0ac03" xlink:to="loc_efsc_OutsideServicesMember_00b9af51-1c3c-40ce-89f5-d881b2fd4fe2" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_OtherExpensesMember_2c261f4e-c67f-422a-8470-aa04d59ba548" xlink:href="efsc-20221231.xsd#efsc_OtherExpensesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterimPeriodCostsNotAllocableDomain_58f3ff12-ca6c-4e87-871e-da28d1a0ac03" xlink:to="loc_efsc_OtherExpensesMember_2c261f4e-c67f-422a-8470-aa04d59ba548" xlink:type="arc" order="6"/>
  </link:definitionLink>
</link:linkbase>
