<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2024 Workiva-->
<!--r:c18644e7-34fa-465d-b778-85f65f3534e4,g:e6c07015-75af-431f-8622-28cbb918af7b-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://www.kns.com/role/DOCUMENTANDENTITYINFORMATION" xlink:type="simple" xlink:href="klic-20240330.xsd#DOCUMENTANDENTITYINFORMATION"/>
  <link:definitionLink xlink:role="http://www.kns.com/role/DOCUMENTANDENTITYINFORMATION" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityAddressesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressesTable_f582600f-8cdd-40ba-beee-4e81fd9334ff" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityAddressesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_EntityAddressesTable_f582600f-8cdd-40ba-beee-4e81fd9334ff" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressesAddressTypeAxis_29389380-4cb5-4cd4-bbb7-0d5b61503bd6" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityAddressesAddressTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_dei_EntityAddressesTable_f582600f-8cdd-40ba-beee-4e81fd9334ff" xlink:to="loc_dei_EntityAddressesAddressTypeAxis_29389380-4cb5-4cd4-bbb7-0d5b61503bd6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_AddressTypeDomain_29389380-4cb5-4cd4-bbb7-0d5b61503bd6_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_AddressTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_EntityAddressesAddressTypeAxis_29389380-4cb5-4cd4-bbb7-0d5b61503bd6" xlink:to="loc_dei_AddressTypeDomain_29389380-4cb5-4cd4-bbb7-0d5b61503bd6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_AddressTypeDomain_9f3b8e16-27ab-44e1-b5a9-fd0180fa9bcf" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_AddressTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_EntityAddressesAddressTypeAxis_29389380-4cb5-4cd4-bbb7-0d5b61503bd6" xlink:to="loc_dei_AddressTypeDomain_9f3b8e16-27ab-44e1-b5a9-fd0180fa9bcf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_OtherAddressMember_09424492-38e5-4358-969e-2fbbb21dd878" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_OtherAddressMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_AddressTypeDomain_9f3b8e16-27ab-44e1-b5a9-fd0180fa9bcf" xlink:to="loc_dei_OtherAddressMember_09424492-38e5-4358-969e-2fbbb21dd878" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentType_837277e6-28fb-49e1-beef-3fd1a73152ca" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_DocumentType"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_DocumentType_837277e6-28fb-49e1-beef-3fd1a73152ca" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentQuarterlyReport_526732c4-44e8-4a86-8ab9-b3732422efdc" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_DocumentQuarterlyReport"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_DocumentQuarterlyReport_526732c4-44e8-4a86-8ab9-b3732422efdc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentTransitionReport_95672acd-197e-4294-8dd3-a5355e3ca033" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_DocumentTransitionReport"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_DocumentTransitionReport_95672acd-197e-4294-8dd3-a5355e3ca033" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFileNumber_313d3f1f-de16-452c-9cca-e64e7d05d562" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityFileNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_EntityFileNumber_313d3f1f-de16-452c-9cca-e64e7d05d562" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCentralIndexKey_fde5b5d8-64c0-4d7a-8bce-cabaae3fdc22" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityCentralIndexKey"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_EntityCentralIndexKey_fde5b5d8-64c0-4d7a-8bce-cabaae3fdc22" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityIncorporationStateCountryCode_ee75d664-8c91-4533-a170-b4d1be26304b" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityIncorporationStateCountryCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_EntityIncorporationStateCountryCode_ee75d664-8c91-4533-a170-b4d1be26304b" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityTaxIdentificationNumber_d80c5545-6d0e-409d-a362-72a68e6bb060" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityTaxIdentificationNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_EntityTaxIdentificationNumber_d80c5545-6d0e-409d-a362-72a68e6bb060" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressAddressLine1_d7556c29-8b28-45ec-ac00-2c075d1dc63a" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityAddressAddressLine1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_EntityAddressAddressLine1_d7556c29-8b28-45ec-ac00-2c075d1dc63a" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressAddressLine2_fb7abb27-400c-45e5-bfa4-b2a5662a998a" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityAddressAddressLine2"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_EntityAddressAddressLine2_fb7abb27-400c-45e5-bfa4-b2a5662a998a" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressCountry_cd7d20bc-24d2-49b7-b3b5-00e91b26a4d1" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityAddressCountry"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_EntityAddressCountry_cd7d20bc-24d2-49b7-b3b5-00e91b26a4d1" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressStateOrProvince_832a1d84-f1f9-4ef1-ae06-e1167a757965" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityAddressStateOrProvince"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_EntityAddressStateOrProvince_832a1d84-f1f9-4ef1-ae06-e1167a757965" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressPostalZipCode_1ac9c132-3ef9-4707-a697-83d2393789e0" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityAddressPostalZipCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_EntityAddressPostalZipCode_1ac9c132-3ef9-4707-a697-83d2393789e0" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CityAreaCode_3fa24d5a-cec5-4a27-90f8-43130f90152e" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_CityAreaCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_CityAreaCode_3fa24d5a-cec5-4a27-90f8-43130f90152e" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LocalPhoneNumber_a8ef8271-183d-40a9-a702-949663109f7a" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LocalPhoneNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_LocalPhoneNumber_a8ef8271-183d-40a9-a702-949663109f7a" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_Security12bTitle_98e43445-27f5-4c0f-a096-36640b033a74" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_Security12bTitle"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_Security12bTitle_98e43445-27f5-4c0f-a096-36640b033a74" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_TradingSymbol_71961f2f-f94b-4e9d-80db-248e01d532a5" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_TradingSymbol"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_TradingSymbol_71961f2f-f94b-4e9d-80db-248e01d532a5" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_SecurityExchangeName_4185dcc2-fc2c-496d-af89-83161b948a70" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_SecurityExchangeName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_SecurityExchangeName_4185dcc2-fc2c-496d-af89-83161b948a70" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCurrentReportingStatus_51dc2b02-8b87-4efe-8672-b5f49c166871" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityCurrentReportingStatus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_EntityCurrentReportingStatus_51dc2b02-8b87-4efe-8672-b5f49c166871" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityInteractiveDataCurrent_79db8289-72f1-4c17-b2fb-1b20151212ce" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityInteractiveDataCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_EntityInteractiveDataCurrent_79db8289-72f1-4c17-b2fb-1b20151212ce" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFilerCategory_f7f861d1-8b3f-4922-b9bd-1654ae3037df" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityFilerCategory"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_EntityFilerCategory_f7f861d1-8b3f-4922-b9bd-1654ae3037df" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntitySmallBusiness_8f9b285e-b416-4ecd-a489-858e76065fe4" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntitySmallBusiness"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_EntitySmallBusiness_8f9b285e-b416-4ecd-a489-858e76065fe4" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityEmergingGrowthCompany_50174263-d67c-47af-a4c5-007c6e121681" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityEmergingGrowthCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_EntityEmergingGrowthCompany_50174263-d67c-47af-a4c5-007c6e121681" xlink:type="arc" order="21"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityShellCompany_bcec0b52-c042-4143-9e3c-04bb9ecbc3bb" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityShellCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_EntityShellCompany_bcec0b52-c042-4143-9e3c-04bb9ecbc3bb" xlink:type="arc" order="22"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCommonStockSharesOutstanding_c0e73334-4bf6-4fc8-86b4-f611776f7011" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityCommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_EntityCommonStockSharesOutstanding_c0e73334-4bf6-4fc8-86b4-f611776f7011" xlink:type="arc" order="23"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_AmendmentFlag_eb996de9-8628-4deb-8c05-3b4ca2249ffc" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_AmendmentFlag"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_AmendmentFlag_eb996de9-8628-4deb-8c05-3b4ca2249ffc" xlink:type="arc" order="24"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentFiscalPeriodFocus_ab716a96-5304-443f-a599-08dfc57a2afa" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_DocumentFiscalPeriodFocus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_DocumentFiscalPeriodFocus_ab716a96-5304-443f-a599-08dfc57a2afa" xlink:type="arc" order="25"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentFiscalYearFocus_af943e1b-b697-43a9-8396-291c876955f4" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_DocumentFiscalYearFocus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_DocumentFiscalYearFocus_af943e1b-b697-43a9-8396-291c876955f4" xlink:type="arc" order="26"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CurrentFiscalYearEndDate_b43c7196-367e-4fde-a87f-4455f5e01360" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_CurrentFiscalYearEndDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_CurrentFiscalYearEndDate_b43c7196-367e-4fde-a87f-4455f5e01360" xlink:type="arc" order="27"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityRegistrantName_08481fa0-95a9-4692-b1f6-78fd1f61aa2d" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityRegistrantName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_EntityRegistrantName_08481fa0-95a9-4692-b1f6-78fd1f61aa2d" xlink:type="arc" order="28"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressCityOrTown_d65cf797-7eab-47b6-9bff-f13f3b5d5c25" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityAddressCityOrTown"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_e5111fa7-d12e-41d2-885b-e628b3473db0" xlink:to="loc_dei_EntityAddressCityOrTown_d65cf797-7eab-47b6-9bff-f13f3b5d5c25" xlink:type="arc" order="29"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.kns.com/role/CONSOLIDATEDCONDENSEDSTATEMENTSOFCHANGESINSHAREHOLDERSEQUITY" xlink:type="simple" xlink:href="klic-20240330.xsd#CONSOLIDATEDCONDENSEDSTATEMENTSOFCHANGESINSHAREHOLDERSEQUITY"/>
  <link:definitionLink xlink:role="http://www.kns.com/role/CONSOLIDATEDCONDENSEDSTATEMENTSOFCHANGESINSHAREHOLDERSEQUITY" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_337359ed-4e76-4f81-936e-d41da1208fee" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_f18fbb44-2400-4715-9a22-b7f9cb9c0c32" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_337359ed-4e76-4f81-936e-d41da1208fee" xlink:to="loc_us-gaap_StatementTable_f18fbb44-2400-4715-9a22-b7f9cb9c0c32" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_6e221f08-1b29-4f4d-8335-76ab60f69f88" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_f18fbb44-2400-4715-9a22-b7f9cb9c0c32" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_6e221f08-1b29-4f4d-8335-76ab60f69f88" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_6e221f08-1b29-4f4d-8335-76ab60f69f88_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_6e221f08-1b29-4f4d-8335-76ab60f69f88" xlink:to="loc_us-gaap_EquityComponentDomain_6e221f08-1b29-4f4d-8335-76ab60f69f88_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_441c17db-21a3-4b90-9933-0cce444b973a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_6e221f08-1b29-4f4d-8335-76ab60f69f88" xlink:to="loc_us-gaap_EquityComponentDomain_441c17db-21a3-4b90-9933-0cce444b973a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_5da3df3c-df35-49ed-a46b-96b04b93a2a7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_441c17db-21a3-4b90-9933-0cce444b973a" xlink:to="loc_us-gaap_CommonStockMember_5da3df3c-df35-49ed-a46b-96b04b93a2a7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockCommonMember_f3392fbe-ff76-4fa9-881e-d95c1f781100" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TreasuryStockCommonMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_441c17db-21a3-4b90-9933-0cce444b973a" xlink:to="loc_us-gaap_TreasuryStockCommonMember_f3392fbe-ff76-4fa9-881e-d95c1f781100" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsMember_152b3bad-6eca-415f-9c83-81641cbfd342" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RetainedEarningsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_441c17db-21a3-4b90-9933-0cce444b973a" xlink:to="loc_us-gaap_RetainedEarningsMember_152b3bad-6eca-415f-9c83-81641cbfd342" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_b803aac2-ce4f-43ad-9855-cdbc4355db5c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_441c17db-21a3-4b90-9933-0cce444b973a" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_b803aac2-ce4f-43ad-9855-cdbc4355db5c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_be69290d-c359-4b31-b287-78e34cda08c2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncreaseDecreaseInStockholdersEquityRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_337359ed-4e76-4f81-936e-d41da1208fee" xlink:to="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_be69290d-c359-4b31-b287-78e34cda08c2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharesOutstanding_05dfdbfb-ee2b-4bd4-bdad-9f39134387d1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_be69290d-c359-4b31-b287-78e34cda08c2" xlink:to="loc_us-gaap_SharesOutstanding_05dfdbfb-ee2b-4bd4-bdad-9f39134387d1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_9f88ca4e-2548-4de7-8cdb-4f246c1c6d00" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_be69290d-c359-4b31-b287-78e34cda08c2" xlink:to="loc_us-gaap_StockholdersEquity_9f88ca4e-2548-4de7-8cdb-4f246c1c6d00" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesIssuedForServices_ae7be2b8-a57b-4eb8-b475-6cb0e68f3fde" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockIssuedDuringPeriodSharesIssuedForServices"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_be69290d-c359-4b31-b287-78e34cda08c2" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesIssuedForServices_ae7be2b8-a57b-4eb8-b475-6cb0e68f3fde" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueIssuedForServices_9b114bef-568f-45e1-8a51-b36c0cf25bc5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockIssuedDuringPeriodValueIssuedForServices"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_be69290d-c359-4b31-b287-78e34cda08c2" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueIssuedForServices_9b114bef-568f-45e1-8a51-b36c0cf25bc5" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockSharesAcquired_c0df1dac-73f1-44db-9c32-87a2b39c735c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TreasuryStockSharesAcquired"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_be69290d-c359-4b31-b287-78e34cda08c2" xlink:to="loc_us-gaap_TreasuryStockSharesAcquired_c0df1dac-73f1-44db-9c32-87a2b39c735c" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockValueAcquiredCostMethod_45eae013-6b7e-46bf-84ff-3e71681e9202" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TreasuryStockValueAcquiredCostMethod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_be69290d-c359-4b31-b287-78e34cda08c2" xlink:to="loc_us-gaap_TreasuryStockValueAcquiredCostMethod_45eae013-6b7e-46bf-84ff-3e71681e9202" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardNetOfForfeitures_1b8870ad-fec2-44b3-a23f-0339d8a7064a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardNetOfForfeitures"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_be69290d-c359-4b31-b287-78e34cda08c2" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardNetOfForfeitures_1b8870ad-fec2-44b3-a23f-0339d8a7064a" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueRestrictedStockAwardNetOfForfeitures_c22b8aa1-7b4b-4f79-b5f2-62c07375709d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockIssuedDuringPeriodValueRestrictedStockAwardNetOfForfeitures"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_be69290d-c359-4b31-b287-78e34cda08c2" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueRestrictedStockAwardNetOfForfeitures_c22b8aa1-7b4b-4f79-b5f2-62c07375709d" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_c9ff7934-3424-4c87-9a3d-ca28538b1684" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_be69290d-c359-4b31-b287-78e34cda08c2" xlink:to="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_c9ff7934-3424-4c87-9a3d-ca28538b1684" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DividendsCommonStockCash_790545d1-1687-4984-850f-184418dc518f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DividendsCommonStockCash"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_be69290d-c359-4b31-b287-78e34cda08c2" xlink:to="loc_us-gaap_DividendsCommonStockCash_790545d1-1687-4984-850f-184418dc518f" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_c346e711-e55c-4478-9c3f-4b7dfb03dcb4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_be69290d-c359-4b31-b287-78e34cda08c2" xlink:to="loc_us-gaap_NetIncomeLoss_c346e711-e55c-4478-9c3f-4b7dfb03dcb4" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_a72938f0-d64d-4d10-ad3e-3a5638a7426a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_be69290d-c359-4b31-b287-78e34cda08c2" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_a72938f0-d64d-4d10-ad3e-3a5638a7426a" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ComprehensiveIncomeNetOfTax_64ceaf11-5f1c-444d-9ff0-9cdb18b8e174" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ComprehensiveIncomeNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_be69290d-c359-4b31-b287-78e34cda08c2" xlink:to="loc_us-gaap_ComprehensiveIncomeNetOfTax_64ceaf11-5f1c-444d-9ff0-9cdb18b8e174" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharesOutstanding_a0fe9189-600e-4462-9523-96e763609834" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SharesOutstanding"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_8ad11020-cba4-499f-8a35-4869fc3f9aa9" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockholdersEquity"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.kns.com/role/GOODWILLANDINTANGIBLEASSETSGoodwillbyReportableSegmentDetails" xlink:type="simple" xlink:href="klic-20240330.xsd#GOODWILLANDINTANGIBLEASSETSGoodwillbyReportableSegmentDetails"/>
  <link:definitionLink xlink:role="http://www.kns.com/role/GOODWILLANDINTANGIBLEASSETSGoodwillbyReportableSegmentDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GoodwillLineItems_0ca1d9fb-9e83-4f92-92bb-8b8adabec0a3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_GoodwillLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfGoodwillTable_744999d2-e323-45f7-93a2-2e4bbf334fac" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfGoodwillTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_GoodwillLineItems_0ca1d9fb-9e83-4f92-92bb-8b8adabec0a3" xlink:to="loc_us-gaap_ScheduleOfGoodwillTable_744999d2-e323-45f7-93a2-2e4bbf334fac" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_a0895c47-c9f2-49da-8802-14bc2fa691cf" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfGoodwillTable_744999d2-e323-45f7-93a2-2e4bbf334fac" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_a0895c47-c9f2-49da-8802-14bc2fa691cf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_a0895c47-c9f2-49da-8802-14bc2fa691cf_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_a0895c47-c9f2-49da-8802-14bc2fa691cf" xlink:to="loc_us-gaap_SegmentDomain_a0895c47-c9f2-49da-8802-14bc2fa691cf_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_907c05fa-63a9-44e3-9706-7757c55b1878" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_a0895c47-c9f2-49da-8802-14bc2fa691cf" xlink:to="loc_us-gaap_SegmentDomain_907c05fa-63a9-44e3-9706-7757c55b1878" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_AllSegmentsExcludingAftermarketProductsAndServicesAPSMember_489efb19-656c-49b6-8457-7d51340cfb45" xlink:href="klic-20240330.xsd#klic_AllSegmentsExcludingAftermarketProductsAndServicesAPSMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_907c05fa-63a9-44e3-9706-7757c55b1878" xlink:to="loc_klic_AllSegmentsExcludingAftermarketProductsAndServicesAPSMember_489efb19-656c-49b6-8457-7d51340cfb45" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_AftermarketProductsandServicesAPSSegmentMember_eeb3cb53-a6b2-4f64-b87f-dfa28f431ca4" xlink:href="klic-20240330.xsd#klic_AftermarketProductsandServicesAPSSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_907c05fa-63a9-44e3-9706-7757c55b1878" xlink:to="loc_klic_AftermarketProductsandServicesAPSSegmentMember_eeb3cb53-a6b2-4f64-b87f-dfa28f431ca4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_AllOthersSegmentMember_05651a74-ac3c-413f-b053-1aba0695fed3" xlink:href="klic-20240330.xsd#klic_AllOthersSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_907c05fa-63a9-44e3-9706-7757c55b1878" xlink:to="loc_klic_AllOthersSegmentMember_05651a74-ac3c-413f-b053-1aba0695fed3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GoodwillRollForward_f61a24c3-4915-4f49-8cfb-08ebe936b14b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_GoodwillRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_GoodwillLineItems_0ca1d9fb-9e83-4f92-92bb-8b8adabec0a3" xlink:to="loc_us-gaap_GoodwillRollForward_f61a24c3-4915-4f49-8cfb-08ebe936b14b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Goodwill_0f4f8a5e-9919-422b-8760-a2364ecf086c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_Goodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_GoodwillRollForward_f61a24c3-4915-4f49-8cfb-08ebe936b14b" xlink:to="loc_us-gaap_Goodwill_0f4f8a5e-9919-422b-8760-a2364ecf086c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GoodwillOtherIncreaseDecrease_9f36b9e5-7028-4d15-a1fb-6dbb637faba7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_GoodwillOtherIncreaseDecrease"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_GoodwillRollForward_f61a24c3-4915-4f49-8cfb-08ebe936b14b" xlink:to="loc_us-gaap_GoodwillOtherIncreaseDecrease_9f36b9e5-7028-4d15-a1fb-6dbb637faba7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Goodwill_8e9299fa-8286-41ff-9693-33c80f9a4ed6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_Goodwill"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_GoodwillCumulativeImpairment_db9cd652-1681-4264-9469-e07899dd09a6" xlink:href="klic-20240330.xsd#klic_GoodwillCumulativeImpairment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_GoodwillRollForward_f61a24c3-4915-4f49-8cfb-08ebe936b14b" xlink:to="loc_klic_GoodwillCumulativeImpairment_db9cd652-1681-4264-9469-e07899dd09a6" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.kns.com/role/GOODWILLANDINTANGIBLEASSETSNetintangibleassetsDetails" xlink:type="simple" xlink:href="klic-20240330.xsd#GOODWILLANDINTANGIBLEASSETSNetintangibleassetsDetails"/>
  <link:definitionLink xlink:role="http://www.kns.com/role/GOODWILLANDINTANGIBLEASSETSNetintangibleassetsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_klic_ScheduleOfIntangibleAssetsLineItems_5a404b63-7c08-409c-9970-f5ff2ab4dc10" xlink:href="klic-20240330.xsd#klic_ScheduleOfIntangibleAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_ScheduleOfIntangibleAssetsTable_fc694d48-6cd1-4b59-bd6d-d6ae80611774" xlink:href="klic-20240330.xsd#klic_ScheduleOfIntangibleAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_klic_ScheduleOfIntangibleAssetsLineItems_5a404b63-7c08-409c-9970-f5ff2ab4dc10" xlink:to="loc_klic_ScheduleOfIntangibleAssetsTable_fc694d48-6cd1-4b59-bd6d-d6ae80611774" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_0add62e5-ffd0-4a8b-a147-c1b2443da4bf" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_klic_ScheduleOfIntangibleAssetsTable_fc694d48-6cd1-4b59-bd6d-d6ae80611774" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_0add62e5-ffd0-4a8b-a147-c1b2443da4bf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_0add62e5-ffd0-4a8b-a147-c1b2443da4bf_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_0add62e5-ffd0-4a8b-a147-c1b2443da4bf" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_0add62e5-ffd0-4a8b-a147-c1b2443da4bf_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_4214a454-d2e9-41a9-bf75-d38c78a67391" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_0add62e5-ffd0-4a8b-a147-c1b2443da4bf" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_4214a454-d2e9-41a9-bf75-d38c78a67391" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DevelopedTechnologyRightsMember_9279e7a1-e4cb-4aa2-b619-244f914e80ac" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DevelopedTechnologyRightsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_4214a454-d2e9-41a9-bf75-d38c78a67391" xlink:to="loc_us-gaap_DevelopedTechnologyRightsMember_9279e7a1-e4cb-4aa2-b619-244f914e80ac" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CustomerRelationshipsMember_43e5879e-0f25-4491-84d5-8ee19b8f98e2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CustomerRelationshipsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_4214a454-d2e9-41a9-bf75-d38c78a67391" xlink:to="loc_us-gaap_CustomerRelationshipsMember_43e5879e-0f25-4491-84d5-8ee19b8f98e2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TradeNamesMember_0082a8ef-68ce-4757-a294-e5851d167202" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TradeNamesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_4214a454-d2e9-41a9-bf75-d38c78a67391" xlink:to="loc_us-gaap_TradeNamesMember_0082a8ef-68ce-4757-a294-e5851d167202" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherIntangibleAssetsMember_3a7f6cfa-a92a-4021-8556-1fa59350f652" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherIntangibleAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_4214a454-d2e9-41a9-bf75-d38c78a67391" xlink:to="loc_us-gaap_OtherIntangibleAssetsMember_3a7f6cfa-a92a-4021-8556-1fa59350f652" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_5ae4e9d4-7f7c-4fa0-929d-ba56354d46cc" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_klic_ScheduleOfIntangibleAssetsTable_fc694d48-6cd1-4b59-bd6d-d6ae80611774" xlink:to="loc_srt_RangeAxis_5ae4e9d4-7f7c-4fa0-929d-ba56354d46cc" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_5ae4e9d4-7f7c-4fa0-929d-ba56354d46cc_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_5ae4e9d4-7f7c-4fa0-929d-ba56354d46cc" xlink:to="loc_srt_RangeMember_5ae4e9d4-7f7c-4fa0-929d-ba56354d46cc_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_7526c69e-80a9-4ff8-8f66-03bf6c1a48bc" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_5ae4e9d4-7f7c-4fa0-929d-ba56354d46cc" xlink:to="loc_srt_RangeMember_7526c69e-80a9-4ff8-8f66-03bf6c1a48bc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_7c25bdac-28b0-4f3f-a034-453a676142a1" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_7526c69e-80a9-4ff8-8f66-03bf6c1a48bc" xlink:to="loc_srt_MinimumMember_7c25bdac-28b0-4f3f-a034-453a676142a1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_67bd56a2-c19b-4f09-8c43-ad7e723251c8" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_7526c69e-80a9-4ff8-8f66-03bf6c1a48bc" xlink:to="loc_srt_MaximumMember_67bd56a2-c19b-4f09-8c43-ad7e723251c8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IndefiniteLivedIntangibleAssetsByMajorClassAxis_b4d82c5a-8b74-4846-bb61-4e108cec8717" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IndefiniteLivedIntangibleAssetsByMajorClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_klic_ScheduleOfIntangibleAssetsTable_fc694d48-6cd1-4b59-bd6d-d6ae80611774" xlink:to="loc_us-gaap_IndefiniteLivedIntangibleAssetsByMajorClassAxis_b4d82c5a-8b74-4846-bb61-4e108cec8717" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IndefiniteLivedIntangibleAssetsMajorClassNameDomain_b4d82c5a-8b74-4846-bb61-4e108cec8717_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IndefiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IndefiniteLivedIntangibleAssetsByMajorClassAxis_b4d82c5a-8b74-4846-bb61-4e108cec8717" xlink:to="loc_us-gaap_IndefiniteLivedIntangibleAssetsMajorClassNameDomain_b4d82c5a-8b74-4846-bb61-4e108cec8717_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IndefiniteLivedIntangibleAssetsMajorClassNameDomain_5640afbd-8adb-4de3-9a5b-fab2dcc0eea0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IndefiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IndefiniteLivedIntangibleAssetsByMajorClassAxis_b4d82c5a-8b74-4846-bb61-4e108cec8717" xlink:to="loc_us-gaap_IndefiniteLivedIntangibleAssetsMajorClassNameDomain_5640afbd-8adb-4de3-9a5b-fab2dcc0eea0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InProcessResearchAndDevelopmentMember_154f7d98-650e-4178-b5fa-808ee855b3f0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_InProcessResearchAndDevelopmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IndefiniteLivedIntangibleAssetsMajorClassNameDomain_5640afbd-8adb-4de3-9a5b-fab2dcc0eea0" xlink:to="loc_us-gaap_InProcessResearchAndDevelopmentMember_154f7d98-650e-4178-b5fa-808ee855b3f0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_bfccff37-1fca-4bfc-9d55-cdf47cc0b2fc" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FiniteLivedIntangibleAssetUsefulLife"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_klic_ScheduleOfIntangibleAssetsLineItems_5a404b63-7c08-409c-9970-f5ff2ab4dc10" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_bfccff37-1fca-4bfc-9d55-cdf47cc0b2fc" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsGross_0364a2a0-c7d9-4b0f-869e-80db98a171a8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FiniteLivedIntangibleAssetsGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_klic_ScheduleOfIntangibleAssetsLineItems_5a404b63-7c08-409c-9970-f5ff2ab4dc10" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsGross_0364a2a0-c7d9-4b0f-869e-80db98a171a8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_22a58cec-0141-4ddd-b3cb-d18a4dc82f17" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_klic_ScheduleOfIntangibleAssetsLineItems_5a404b63-7c08-409c-9970-f5ff2ab4dc10" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_22a58cec-0141-4ddd-b3cb-d18a4dc82f17" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IntangibleAssetsNetExcludingGoodwill_42643910-c561-4971-9da9-8b924fd676f6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IntangibleAssetsNetExcludingGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_klic_ScheduleOfIntangibleAssetsLineItems_5a404b63-7c08-409c-9970-f5ff2ab4dc10" xlink:to="loc_us-gaap_IntangibleAssetsNetExcludingGoodwill_42643910-c561-4971-9da9-8b924fd676f6" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.kns.com/role/CASHCASHEQUIVALENTSRESTRICTEDCASHANDSHORTTERMINVESTMENTSDetails" xlink:type="simple" xlink:href="klic-20240330.xsd#CASHCASHEQUIVALENTSRESTRICTEDCASHANDSHORTTERMINVESTMENTSDetails"/>
  <link:definitionLink xlink:role="http://www.kns.com/role/CASHCASHEQUIVALENTSRESTRICTEDCASHANDSHORTTERMINVESTMENTSDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsLineItems_6f996c39-1335-40d3-9754-8095f7e408cc" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CashAndCashEquivalentsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfCashAndCashEquivalentsTable_f1d18b1e-2f72-4692-9453-0d0e6e0883a3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfCashAndCashEquivalentsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_6f996c39-1335-40d3-9754-8095f7e408cc" xlink:to="loc_us-gaap_ScheduleOfCashAndCashEquivalentsTable_f1d18b1e-2f72-4692-9453-0d0e6e0883a3" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentTypeAxis_db364d25-f6d5-49ac-8c24-9eeef55d3b0f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_InvestmentTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfCashAndCashEquivalentsTable_f1d18b1e-2f72-4692-9453-0d0e6e0883a3" xlink:to="loc_us-gaap_InvestmentTypeAxis_db364d25-f6d5-49ac-8c24-9eeef55d3b0f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentTypeCategorizationMember_db364d25-f6d5-49ac-8c24-9eeef55d3b0f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_InvestmentTypeCategorizationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_InvestmentTypeAxis_db364d25-f6d5-49ac-8c24-9eeef55d3b0f" xlink:to="loc_us-gaap_InvestmentTypeCategorizationMember_db364d25-f6d5-49ac-8c24-9eeef55d3b0f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentTypeCategorizationMember_b77f4123-9db6-4a55-b725-d204b3c115bf" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_InvestmentTypeCategorizationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_InvestmentTypeAxis_db364d25-f6d5-49ac-8c24-9eeef55d3b0f" xlink:to="loc_us-gaap_InvestmentTypeCategorizationMember_b77f4123-9db6-4a55-b725-d204b3c115bf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MoneyMarketFundsMember_989c1696-8395-4d52-871f-259a0ad9029d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_MoneyMarketFundsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InvestmentTypeCategorizationMember_b77f4123-9db6-4a55-b725-d204b3c115bf" xlink:to="loc_us-gaap_MoneyMarketFundsMember_989c1696-8395-4d52-871f-259a0ad9029d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BankTimeDepositsMember_bc7f8a60-dc80-4e53-9f99-c47bbd396aba" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_BankTimeDepositsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InvestmentTypeCategorizationMember_b77f4123-9db6-4a55-b725-d204b3c115bf" xlink:to="loc_us-gaap_BankTimeDepositsMember_bc7f8a60-dc80-4e53-9f99-c47bbd396aba" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Cash_5afe00d8-19cf-43a0-bff8-d2410bc429a5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_Cash"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_6f996c39-1335-40d3-9754-8095f7e408cc" xlink:to="loc_us-gaap_Cash_5afe00d8-19cf-43a0-bff8-d2410bc429a5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_CashUnrealizedGains_734411c5-5a5d-4b33-b049-1f745e760abc" xlink:href="klic-20240330.xsd#klic_CashUnrealizedGains"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_6f996c39-1335-40d3-9754-8095f7e408cc" xlink:to="loc_klic_CashUnrealizedGains_734411c5-5a5d-4b33-b049-1f745e760abc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_CashUnrealizedLosses_1ad051e0-f07f-4eb7-bf3b-ecb92db5dfab" xlink:href="klic-20240330.xsd#klic_CashUnrealizedLosses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_6f996c39-1335-40d3-9754-8095f7e408cc" xlink:to="loc_klic_CashUnrealizedLosses_1ad051e0-f07f-4eb7-bf3b-ecb92db5dfab" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_CashEstimatedFairValue_625e2db3-e2e3-470b-b19a-5cddda821cf2" xlink:href="klic-20240330.xsd#klic_CashEstimatedFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_6f996c39-1335-40d3-9754-8095f7e408cc" xlink:to="loc_klic_CashEstimatedFairValue_625e2db3-e2e3-470b-b19a-5cddda821cf2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_CashEquivalentsAmortizedCost_91706f00-db18-4801-9a70-a6e2a6259569" xlink:href="klic-20240330.xsd#klic_CashEquivalentsAmortizedCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_6f996c39-1335-40d3-9754-8095f7e408cc" xlink:to="loc_klic_CashEquivalentsAmortizedCost_91706f00-db18-4801-9a70-a6e2a6259569" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_CashEquivalentsGrossUnrealizedGain_768c2732-ad16-4b73-a554-4e63ae6e6024" xlink:href="klic-20240330.xsd#klic_CashEquivalentsGrossUnrealizedGain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_6f996c39-1335-40d3-9754-8095f7e408cc" xlink:to="loc_klic_CashEquivalentsGrossUnrealizedGain_768c2732-ad16-4b73-a554-4e63ae6e6024" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_CashEquivalentsGrossUnrealizedLoss_aa921336-804f-4ec0-a50e-b38559024937" xlink:href="klic-20240330.xsd#klic_CashEquivalentsGrossUnrealizedLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_6f996c39-1335-40d3-9754-8095f7e408cc" xlink:to="loc_klic_CashEquivalentsGrossUnrealizedLoss_aa921336-804f-4ec0-a50e-b38559024937" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_CashEquivalentsFairValueDisclosure_c64ac421-5fe5-425c-b92a-7e2709ee14c3" xlink:href="klic-20240330.xsd#klic_CashEquivalentsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_6f996c39-1335-40d3-9754-8095f7e408cc" xlink:to="loc_klic_CashEquivalentsFairValueDisclosure_c64ac421-5fe5-425c-b92a-7e2709ee14c3" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_CashandCashEquivalentsAmortizedCost_d9d26bcd-b064-473c-806c-7c25d2fd886b" xlink:href="klic-20240330.xsd#klic_CashandCashEquivalentsAmortizedCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_6f996c39-1335-40d3-9754-8095f7e408cc" xlink:to="loc_klic_CashandCashEquivalentsAmortizedCost_d9d26bcd-b064-473c-806c-7c25d2fd886b" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_CashandCashEquivalentsGrossUnrealizedGain_c3f86d2f-a6c6-4d08-8f72-b5ee285d75c5" xlink:href="klic-20240330.xsd#klic_CashandCashEquivalentsGrossUnrealizedGain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_6f996c39-1335-40d3-9754-8095f7e408cc" xlink:to="loc_klic_CashandCashEquivalentsGrossUnrealizedGain_c3f86d2f-a6c6-4d08-8f72-b5ee285d75c5" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_CashandCashEquivalentsGrossUnrealizedLoss_0dbdc159-680b-469d-a818-a3c6b4f4da16" xlink:href="klic-20240330.xsd#klic_CashandCashEquivalentsGrossUnrealizedLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_6f996c39-1335-40d3-9754-8095f7e408cc" xlink:to="loc_klic_CashandCashEquivalentsGrossUnrealizedLoss_0dbdc159-680b-469d-a818-a3c6b4f4da16" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_f56222d8-b525-42a5-8c9a-102aacf9a628" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CashAndCashEquivalentsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_6f996c39-1335-40d3-9754-8095f7e408cc" xlink:to="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_f56222d8-b525-42a5-8c9a-102aacf9a628" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShortTermInvestments_34d221cd-c5b7-43f9-ba76-1fefa2823fac" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShortTermInvestments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_6f996c39-1335-40d3-9754-8095f7e408cc" xlink:to="loc_us-gaap_ShortTermInvestments_34d221cd-c5b7-43f9-ba76-1fefa2823fac" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_AvailableforsaleSecuritiesShorttermInvestmentsGrossUnrealizedGain_f2aad5b0-2ba0-4719-890e-c45921f272d6" xlink:href="klic-20240330.xsd#klic_AvailableforsaleSecuritiesShorttermInvestmentsGrossUnrealizedGain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_6f996c39-1335-40d3-9754-8095f7e408cc" xlink:to="loc_klic_AvailableforsaleSecuritiesShorttermInvestmentsGrossUnrealizedGain_f2aad5b0-2ba0-4719-890e-c45921f272d6" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_AvailableforsaleSecuritiesShorttermInvestmentsGrossUnrealizedLoss_5a7403b3-575a-4c5c-9e07-051952e3ed29" xlink:href="klic-20240330.xsd#klic_AvailableforsaleSecuritiesShorttermInvestmentsGrossUnrealizedLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_6f996c39-1335-40d3-9754-8095f7e408cc" xlink:to="loc_klic_AvailableforsaleSecuritiesShorttermInvestmentsGrossUnrealizedLoss_5a7403b3-575a-4c5c-9e07-051952e3ed29" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_AvailableforsaleSecuritiesShorttermInvestmentsFairValueDisclosure_0acb9f82-635c-4fb7-91c2-04c576373fc3" xlink:href="klic-20240330.xsd#klic_AvailableforsaleSecuritiesShorttermInvestmentsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_6f996c39-1335-40d3-9754-8095f7e408cc" xlink:to="loc_klic_AvailableforsaleSecuritiesShorttermInvestmentsFairValueDisclosure_0acb9f82-635c-4fb7-91c2-04c576373fc3" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_CashCashEquivalentsRestrictedCashRestrictedCashEquivalentsandShortTermInvestments_2e5b10fe-76ae-42a2-8968-af25ec1613d5" xlink:href="klic-20240330.xsd#klic_CashCashEquivalentsRestrictedCashRestrictedCashEquivalentsandShortTermInvestments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_6f996c39-1335-40d3-9754-8095f7e408cc" xlink:to="loc_klic_CashCashEquivalentsRestrictedCashRestrictedCashEquivalentsandShortTermInvestments_2e5b10fe-76ae-42a2-8968-af25ec1613d5" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_CashCashEquivalentsRestrictedCashRestrictedCashEquivalentsandShortTermInvestmentsGrossUnrealizedGain_58e77f8d-080e-47ee-ae97-e458eaa47caf" xlink:href="klic-20240330.xsd#klic_CashCashEquivalentsRestrictedCashRestrictedCashEquivalentsandShortTermInvestmentsGrossUnrealizedGain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_6f996c39-1335-40d3-9754-8095f7e408cc" xlink:to="loc_klic_CashCashEquivalentsRestrictedCashRestrictedCashEquivalentsandShortTermInvestmentsGrossUnrealizedGain_58e77f8d-080e-47ee-ae97-e458eaa47caf" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_CashCashEquivalentsRestrictedCashRestrictedCashEquivalentsandShortTermInvestmentsGrossUnrealizedLoss_d236b2c3-d7f5-4e10-b656-244196a44b07" xlink:href="klic-20240330.xsd#klic_CashCashEquivalentsRestrictedCashRestrictedCashEquivalentsandShortTermInvestmentsGrossUnrealizedLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_6f996c39-1335-40d3-9754-8095f7e408cc" xlink:to="loc_klic_CashCashEquivalentsRestrictedCashRestrictedCashEquivalentsandShortTermInvestmentsGrossUnrealizedLoss_d236b2c3-d7f5-4e10-b656-244196a44b07" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_CashCashEquivalentsRestrictedCashRestrictedCashEquivalentsandShortTermInvestmentsFairValueDisclosure_1a0a7fad-c398-49a9-913d-e82ed1b094cc" xlink:href="klic-20240330.xsd#klic_CashCashEquivalentsRestrictedCashRestrictedCashEquivalentsandShortTermInvestmentsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_6f996c39-1335-40d3-9754-8095f7e408cc" xlink:to="loc_klic_CashCashEquivalentsRestrictedCashRestrictedCashEquivalentsandShortTermInvestmentsFairValueDisclosure_1a0a7fad-c398-49a9-913d-e82ed1b094cc" xlink:type="arc" order="19"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.kns.com/role/DERIVATIVESFINANCIALINSTRUMENTSFairvalueofderivativeinstrumentsDetails" xlink:type="simple" xlink:href="klic-20240330.xsd#DERIVATIVESFINANCIALINSTRUMENTSFairvalueofderivativeinstrumentsDetails"/>
  <link:definitionLink xlink:role="http://www.kns.com/role/DERIVATIVESFINANCIALINSTRUMENTSFairvalueofderivativeinstrumentsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativesFairValueLineItems_6561a027-0310-46e5-b598-872ed67fa05c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativesFairValueLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValuesDerivativesBalanceSheetLocationByDerivativeContractTypeByHedgingDesignationTable_569e4f4b-ff8e-4a3b-8d26-7428823ae7bd" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValuesDerivativesBalanceSheetLocationByDerivativeContractTypeByHedgingDesignationTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DerivativesFairValueLineItems_6561a027-0310-46e5-b598-872ed67fa05c" xlink:to="loc_us-gaap_FairValuesDerivativesBalanceSheetLocationByDerivativeContractTypeByHedgingDesignationTable_569e4f4b-ff8e-4a3b-8d26-7428823ae7bd" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BalanceSheetLocationAxis_4a12ec82-1e79-44a4-a238-cc212614ce94" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_BalanceSheetLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValuesDerivativesBalanceSheetLocationByDerivativeContractTypeByHedgingDesignationTable_569e4f4b-ff8e-4a3b-8d26-7428823ae7bd" xlink:to="loc_us-gaap_BalanceSheetLocationAxis_4a12ec82-1e79-44a4-a238-cc212614ce94" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BalanceSheetLocationDomain_4a12ec82-1e79-44a4-a238-cc212614ce94_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_BalanceSheetLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BalanceSheetLocationAxis_4a12ec82-1e79-44a4-a238-cc212614ce94" xlink:to="loc_us-gaap_BalanceSheetLocationDomain_4a12ec82-1e79-44a4-a238-cc212614ce94_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BalanceSheetLocationDomain_ee86eba1-95a2-49e3-84a6-1b3ef0772b5a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_BalanceSheetLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BalanceSheetLocationAxis_4a12ec82-1e79-44a4-a238-cc212614ce94" xlink:to="loc_us-gaap_BalanceSheetLocationDomain_ee86eba1-95a2-49e3-84a6-1b3ef0772b5a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_AccruedExpensesAndOtherCurrentLiabilitiesMember_d7695c59-05e8-4a0f-a1b2-f48dc148939e" xlink:href="klic-20240330.xsd#klic_AccruedExpensesAndOtherCurrentLiabilitiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BalanceSheetLocationDomain_ee86eba1-95a2-49e3-84a6-1b3ef0772b5a" xlink:to="loc_klic_AccruedExpensesAndOtherCurrentLiabilitiesMember_d7695c59-05e8-4a0f-a1b2-f48dc148939e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HedgingDesignationAxis_922d069a-5e24-4ead-891c-aac8b6cdeaeb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_HedgingDesignationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValuesDerivativesBalanceSheetLocationByDerivativeContractTypeByHedgingDesignationTable_569e4f4b-ff8e-4a3b-8d26-7428823ae7bd" xlink:to="loc_us-gaap_HedgingDesignationAxis_922d069a-5e24-4ead-891c-aac8b6cdeaeb" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HedgingDesignationDomain_922d069a-5e24-4ead-891c-aac8b6cdeaeb_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_HedgingDesignationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_HedgingDesignationAxis_922d069a-5e24-4ead-891c-aac8b6cdeaeb" xlink:to="loc_us-gaap_HedgingDesignationDomain_922d069a-5e24-4ead-891c-aac8b6cdeaeb_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HedgingDesignationDomain_f6cfb700-ab1a-422b-9be9-f7ea701ae46c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_HedgingDesignationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_HedgingDesignationAxis_922d069a-5e24-4ead-891c-aac8b6cdeaeb" xlink:to="loc_us-gaap_HedgingDesignationDomain_f6cfb700-ab1a-422b-9be9-f7ea701ae46c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DesignatedAsHedgingInstrumentMember_3e1cb102-5702-4540-b754-18231049781a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DesignatedAsHedgingInstrumentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HedgingDesignationDomain_f6cfb700-ab1a-422b-9be9-f7ea701ae46c" xlink:to="loc_us-gaap_DesignatedAsHedgingInstrumentMember_3e1cb102-5702-4540-b754-18231049781a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeInstrumentRiskAxis_0616950e-eb87-43cd-8d1c-ac50a4b6ac83" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeInstrumentRiskAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValuesDerivativesBalanceSheetLocationByDerivativeContractTypeByHedgingDesignationTable_569e4f4b-ff8e-4a3b-8d26-7428823ae7bd" xlink:to="loc_us-gaap_DerivativeInstrumentRiskAxis_0616950e-eb87-43cd-8d1c-ac50a4b6ac83" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_0616950e-eb87-43cd-8d1c-ac50a4b6ac83_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_0616950e-eb87-43cd-8d1c-ac50a4b6ac83" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_0616950e-eb87-43cd-8d1c-ac50a4b6ac83_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_a7c9f55c-abed-4c88-8295-a10a2303b21d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_0616950e-eb87-43cd-8d1c-ac50a4b6ac83" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_a7c9f55c-abed-4c88-8295-a10a2303b21d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ForeignExchangeForwardMember_026a5ff8-035a-4294-82ba-ee1c03a6ed5d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ForeignExchangeForwardMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeContractTypeDomain_a7c9f55c-abed-4c88-8295-a10a2303b21d" xlink:to="loc_us-gaap_ForeignExchangeForwardMember_026a5ff8-035a-4294-82ba-ee1c03a6ed5d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeTermOfContract_9a1984b6-96c1-42dd-b7e4-67a75c675964" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeTermOfContract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativesFairValueLineItems_6561a027-0310-46e5-b598-872ed67fa05c" xlink:to="loc_us-gaap_DerivativeTermOfContract_9a1984b6-96c1-42dd-b7e4-67a75c675964" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeNotionalAmount_b7f9e61d-2d8c-40ab-87cf-c12821dc00a0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeNotionalAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativesFairValueLineItems_6561a027-0310-46e5-b598-872ed67fa05c" xlink:to="loc_us-gaap_DerivativeNotionalAmount_b7f9e61d-2d8c-40ab-87cf-c12821dc00a0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeFairValueOfDerivativeLiability_253ea55e-fe75-4697-b4e4-ab2d7e52a038" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeFairValueOfDerivativeLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativesFairValueLineItems_6561a027-0310-46e5-b598-872ed67fa05c" xlink:to="loc_us-gaap_DerivativeFairValueOfDerivativeLiability_253ea55e-fe75-4697-b4e4-ab2d7e52a038" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeInstrumentsGainLossReclassificationFromAccumulatedOCIToIncomeEstimateOfTimeToTransfer1_cee9d85c-bed9-4a29-b9b7-0143f22282c7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeInstrumentsGainLossReclassificationFromAccumulatedOCIToIncomeEstimateOfTimeToTransfer1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativesFairValueLineItems_6561a027-0310-46e5-b598-872ed67fa05c" xlink:to="loc_us-gaap_DerivativeInstrumentsGainLossReclassificationFromAccumulatedOCIToIncomeEstimateOfTimeToTransfer1_cee9d85c-bed9-4a29-b9b7-0143f22282c7" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.kns.com/role/DERIVATIVESFINANCIALINSTRUMENTSGainlossofderivativeinstrumentsDetails" xlink:type="simple" xlink:href="klic-20240330.xsd#DERIVATIVESFINANCIALINSTRUMENTSGainlossofderivativeinstrumentsDetails"/>
  <link:definitionLink xlink:role="http://www.kns.com/role/DERIVATIVESFINANCIALINSTRUMENTSGainlossofderivativeinstrumentsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeInstrumentsGainLossLineItems_e86f00e1-926f-4df4-90e7-2cb67f0d9ad9" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeInstrumentsGainLossLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeInstrumentsGainLossByHedgingRelationshipByIncomeStatementLocationByDerivativeInstrumentRiskTable_ca579747-7406-474e-be10-e10d46768e5f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeInstrumentsGainLossByHedgingRelationshipByIncomeStatementLocationByDerivativeInstrumentRiskTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DerivativeInstrumentsGainLossLineItems_e86f00e1-926f-4df4-90e7-2cb67f0d9ad9" xlink:to="loc_us-gaap_DerivativeInstrumentsGainLossByHedgingRelationshipByIncomeStatementLocationByDerivativeInstrumentRiskTable_ca579747-7406-474e-be10-e10d46768e5f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_c9be7aef-1e37-4ab9-a7d5-88617e71cca2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeInstrumentsGainLossByHedgingRelationshipByIncomeStatementLocationByDerivativeInstrumentRiskTable_ca579747-7406-474e-be10-e10d46768e5f" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_c9be7aef-1e37-4ab9-a7d5-88617e71cca2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_c9be7aef-1e37-4ab9-a7d5-88617e71cca2_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_c9be7aef-1e37-4ab9-a7d5-88617e71cca2" xlink:to="loc_us-gaap_EquityComponentDomain_c9be7aef-1e37-4ab9-a7d5-88617e71cca2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_ee111d8d-fc13-43e4-826d-7505ec07dd9f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_c9be7aef-1e37-4ab9-a7d5-88617e71cca2" xlink:to="loc_us-gaap_EquityComponentDomain_ee111d8d-fc13-43e4-826d-7505ec07dd9f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedGainLossNetCashFlowHedgeParentMember_67f9ab1b-4d6c-4503-978e-2306cb8d30f9" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccumulatedGainLossNetCashFlowHedgeParentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_ee111d8d-fc13-43e4-826d-7505ec07dd9f" xlink:to="loc_us-gaap_AccumulatedGainLossNetCashFlowHedgeParentMember_67f9ab1b-4d6c-4503-978e-2306cb8d30f9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossCashFlowHedgeGainLossBeforeReclassificationAfterTax_c09e908f-4488-4b37-900a-aadde48c5474" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherComprehensiveIncomeLossCashFlowHedgeGainLossBeforeReclassificationAfterTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeInstrumentsGainLossLineItems_e86f00e1-926f-4df4-90e7-2cb67f0d9ad9" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossCashFlowHedgeGainLossBeforeReclassificationAfterTax_c09e908f-4488-4b37-900a-aadde48c5474" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossCashFlowHedgeGainLossReclassificationAfterTax_d9934510-8112-4ef0-8727-f851b7da1486" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherComprehensiveIncomeLossCashFlowHedgeGainLossReclassificationAfterTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeInstrumentsGainLossLineItems_e86f00e1-926f-4df4-90e7-2cb67f0d9ad9" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossCashFlowHedgeGainLossReclassificationAfterTax_d9934510-8112-4ef0-8727-f851b7da1486" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.kns.com/role/LEASESNarrativeDetails" xlink:type="simple" xlink:href="klic-20240330.xsd#LEASESNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.kns.com/role/LEASESNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeLeaseDescriptionLineItems_2a4a2e12-0051-4cbb-aae3-f5758e0a636e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LesseeLeaseDescriptionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeLeaseDescriptionTable_077dfd18-69ec-4f36-bf61-a9dd1e4f1102" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LesseeLeaseDescriptionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_2a4a2e12-0051-4cbb-aae3-f5758e0a636e" xlink:to="loc_us-gaap_LesseeLeaseDescriptionTable_077dfd18-69ec-4f36-bf61-a9dd1e4f1102" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_59edc610-8d1d-4c1c-9266-b0c5c4ae5e16" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LesseeLeaseDescriptionTable_077dfd18-69ec-4f36-bf61-a9dd1e4f1102" xlink:to="loc_srt_RangeAxis_59edc610-8d1d-4c1c-9266-b0c5c4ae5e16" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_59edc610-8d1d-4c1c-9266-b0c5c4ae5e16_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_59edc610-8d1d-4c1c-9266-b0c5c4ae5e16" xlink:to="loc_srt_RangeMember_59edc610-8d1d-4c1c-9266-b0c5c4ae5e16_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_c408e964-3b56-4abe-9e91-e5158737b563" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_59edc610-8d1d-4c1c-9266-b0c5c4ae5e16" xlink:to="loc_srt_RangeMember_c408e964-3b56-4abe-9e91-e5158737b563" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_79c3d8be-c45d-494a-9815-2552805b0516" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_c408e964-3b56-4abe-9e91-e5158737b563" xlink:to="loc_srt_MinimumMember_79c3d8be-c45d-494a-9815-2552805b0516" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_19efc783-da5c-4cfd-86d5-b73df2161b03" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_c408e964-3b56-4abe-9e91-e5158737b563" xlink:to="loc_srt_MaximumMember_19efc783-da5c-4cfd-86d5-b73df2161b03" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseTermOfContract_80c2f078-30b4-467e-ac72-3433c17d7c9c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LesseeOperatingLeaseTermOfContract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_2a4a2e12-0051-4cbb-aae3-f5758e0a636e" xlink:to="loc_us-gaap_LesseeOperatingLeaseTermOfContract_80c2f078-30b4-467e-ac72-3433c17d7c9c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_LeasesNumberofOptionsToExtend_eaa18977-8da9-4953-b722-498943d00da5" xlink:href="klic-20240330.xsd#klic_LeasesNumberofOptionsToExtend"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LesseeLeaseDescriptionLineItems_2a4a2e12-0051-4cbb-aae3-f5758e0a636e" xlink:to="loc_klic_LeasesNumberofOptionsToExtend_eaa18977-8da9-4953-b722-498943d00da5" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.kns.com/role/DEBTANDOTHEROBLIGATIONSDEBTANDOTHEROBLIGATIONSDetails" xlink:type="simple" xlink:href="klic-20240330.xsd#DEBTANDOTHEROBLIGATIONSDEBTANDOTHEROBLIGATIONSDetails"/>
  <link:definitionLink xlink:role="http://www.kns.com/role/DEBTANDOTHEROBLIGATIONSDEBTANDOTHEROBLIGATIONSDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CapitalLeasedAssetsLineItems_934b3b88-58b9-47a6-aeff-64e71da2d96d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CapitalLeasedAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfCapitalLeasedAsssetsTable_93de3a38-944c-46d7-bb92-70c69b358a0b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfCapitalLeasedAsssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_CapitalLeasedAssetsLineItems_934b3b88-58b9-47a6-aeff-64e71da2d96d" xlink:to="loc_us-gaap_ScheduleOfCapitalLeasedAsssetsTable_93de3a38-944c-46d7-bb92-70c69b358a0b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateAxis_e89b0b42-912f-49ae-949e-f027d7b83e9e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_VariableRateAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfCapitalLeasedAsssetsTable_93de3a38-944c-46d7-bb92-70c69b358a0b" xlink:to="loc_us-gaap_VariableRateAxis_e89b0b42-912f-49ae-949e-f027d7b83e9e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_e89b0b42-912f-49ae-949e-f027d7b83e9e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_VariableRateAxis_e89b0b42-912f-49ae-949e-f027d7b83e9e" xlink:to="loc_us-gaap_VariableRateDomain_e89b0b42-912f-49ae-949e-f027d7b83e9e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_e171956f-8188-4455-bc43-d11d915ab327" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_VariableRateAxis_e89b0b42-912f-49ae-949e-f027d7b83e9e" xlink:to="loc_us-gaap_VariableRateDomain_e171956f-8188-4455-bc43-d11d915ab327" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuredOvernightFinancingRateSofrOvernightIndexSwapRateMember_66a8d0e0-97c3-466f-aa81-e3c74f53d4e2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SecuredOvernightFinancingRateSofrOvernightIndexSwapRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableRateDomain_e171956f-8188-4455-bc43-d11d915ab327" xlink:to="loc_us-gaap_SecuredOvernightFinancingRateSofrOvernightIndexSwapRateMember_66a8d0e0-97c3-466f-aa81-e3c74f53d4e2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_02ccbae1-2364-4a9b-95b9-27efedb2264f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfCapitalLeasedAsssetsTable_93de3a38-944c-46d7-bb92-70c69b358a0b" xlink:to="loc_us-gaap_DebtInstrumentAxis_02ccbae1-2364-4a9b-95b9-27efedb2264f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_02ccbae1-2364-4a9b-95b9-27efedb2264f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_02ccbae1-2364-4a9b-95b9-27efedb2264f" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_02ccbae1-2364-4a9b-95b9-27efedb2264f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_9b6af099-99db-4343-9b21-4e97705109fc" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_02ccbae1-2364-4a9b-95b9-27efedb2264f" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_9b6af099-99db-4343-9b21-4e97705109fc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_FacilityAgreementsMember_998c8531-6e29-44c5-aed2-508a43e45af5" xlink:href="klic-20240330.xsd#klic_FacilityAgreementsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_9b6af099-99db-4343-9b21-4e97705109fc" xlink:to="loc_klic_FacilityAgreementsMember_998c8531-6e29-44c5-aed2-508a43e45af5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityAxis_153a2ae1-440a-41db-91db-e4b64e22f458" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfCapitalLeasedAsssetsTable_93de3a38-944c-46d7-bb92-70c69b358a0b" xlink:to="loc_us-gaap_LineOfCreditFacilityAxis_153a2ae1-440a-41db-91db-e4b64e22f458" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityLenderDomain_153a2ae1-440a-41db-91db-e4b64e22f458_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditFacilityLenderDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LineOfCreditFacilityAxis_153a2ae1-440a-41db-91db-e4b64e22f458" xlink:to="loc_us-gaap_LineOfCreditFacilityLenderDomain_153a2ae1-440a-41db-91db-e4b64e22f458_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityLenderDomain_3312272a-711b-42dc-ba57-5646a91e8138" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditFacilityLenderDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LineOfCreditFacilityAxis_153a2ae1-440a-41db-91db-e4b64e22f458" xlink:to="loc_us-gaap_LineOfCreditFacilityLenderDomain_3312272a-711b-42dc-ba57-5646a91e8138" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_CitibankMember_7999b715-d9f0-4029-9be2-3b1aaa402d22" xlink:href="klic-20240330.xsd#klic_CitibankMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLenderDomain_3312272a-711b-42dc-ba57-5646a91e8138" xlink:to="loc_klic_CitibankMember_7999b715-d9f0-4029-9be2-3b1aaa402d22" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_MUFGBankLtd.SingaporeBranchMember_512ce249-c00f-4d24-beba-a70a6e249506" xlink:href="klic-20240330.xsd#klic_MUFGBankLtd.SingaporeBranchMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLenderDomain_3312272a-711b-42dc-ba57-5646a91e8138" xlink:to="loc_klic_MUFGBankLtd.SingaporeBranchMember_512ce249-c00f-4d24-beba-a70a6e249506" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_dd8c3ab9-9ed1-483f-9a05-b60c062d3e07" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CapitalLeasedAssetsLineItems_934b3b88-58b9-47a6-aeff-64e71da2d96d" xlink:to="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_dd8c3ab9-9ed1-483f-9a05-b60c062d3e07" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCredit_ac5c0d09-7482-42a8-adf8-d5030c1612c3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCredit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CapitalLeasedAssetsLineItems_934b3b88-58b9-47a6-aeff-64e71da2d96d" xlink:to="loc_us-gaap_LineOfCredit_ac5c0d09-7482-42a8-adf8-d5030c1612c3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_4c9c9fdc-f37c-404a-8515-c330bdde38fb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentBasisSpreadOnVariableRate1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CapitalLeasedAssetsLineItems_934b3b88-58b9-47a6-aeff-64e71da2d96d" xlink:to="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_4c9c9fdc-f37c-404a-8515-c330bdde38fb" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.kns.com/role/SHAREHOLDERSEQUITYANDEMPLOYEEBENEFITPLANSNarrativeDetails" xlink:type="simple" xlink:href="klic-20240330.xsd#SHAREHOLDERSEQUITYANDEMPLOYEEBENEFITPLANSNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.kns.com/role/SHAREHOLDERSEQUITYANDEMPLOYEEBENEFITPLANSNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_451a7206-9fb3-49ab-a9e7-bcd4246158d3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_76314a28-b0b0-489b-817a-57615a572ef3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_451a7206-9fb3-49ab-a9e7-bcd4246158d3" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_76314a28-b0b0-489b-817a-57615a572ef3" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_6f5f2393-26b8-4979-8f7e-a4b9ddbd8d82" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_76314a28-b0b0-489b-817a-57615a572ef3" xlink:to="loc_srt_RangeAxis_6f5f2393-26b8-4979-8f7e-a4b9ddbd8d82" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_6f5f2393-26b8-4979-8f7e-a4b9ddbd8d82_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_6f5f2393-26b8-4979-8f7e-a4b9ddbd8d82" xlink:to="loc_srt_RangeMember_6f5f2393-26b8-4979-8f7e-a4b9ddbd8d82_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_9a9a1db0-735d-45fb-996e-29baeff21cc9" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_6f5f2393-26b8-4979-8f7e-a4b9ddbd8d82" xlink:to="loc_srt_RangeMember_9a9a1db0-735d-45fb-996e-29baeff21cc9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_f154db1b-8f9f-4dda-bfa0-3c0d1745db63" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_9a9a1db0-735d-45fb-996e-29baeff21cc9" xlink:to="loc_srt_MinimumMember_f154db1b-8f9f-4dda-bfa0-3c0d1745db63" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_61b01d0e-370d-4f95-902e-d0cde7d2473f" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_9a9a1db0-735d-45fb-996e-29baeff21cc9" xlink:to="loc_srt_MaximumMember_61b01d0e-370d-4f95-902e-d0cde7d2473f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareRepurchaseProgramAxis_10539a5d-3dfd-4113-a465-cc118d9b9d03" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareRepurchaseProgramAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_76314a28-b0b0-489b-817a-57615a572ef3" xlink:to="loc_us-gaap_ShareRepurchaseProgramAxis_10539a5d-3dfd-4113-a465-cc118d9b9d03" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareRepurchaseProgramDomain_10539a5d-3dfd-4113-a465-cc118d9b9d03_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareRepurchaseProgramDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ShareRepurchaseProgramAxis_10539a5d-3dfd-4113-a465-cc118d9b9d03" xlink:to="loc_us-gaap_ShareRepurchaseProgramDomain_10539a5d-3dfd-4113-a465-cc118d9b9d03_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareRepurchaseProgramDomain_968e65b8-f652-4c9f-bc58-98a97f2ba93a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareRepurchaseProgramDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ShareRepurchaseProgramAxis_10539a5d-3dfd-4113-a465-cc118d9b9d03" xlink:to="loc_us-gaap_ShareRepurchaseProgramDomain_968e65b8-f652-4c9f-bc58-98a97f2ba93a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_ProgramAuthorized15August2017Member_14f40c65-dbc0-4739-b9a8-742462527897" xlink:href="klic-20240330.xsd#klic_ProgramAuthorized15August2017Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareRepurchaseProgramDomain_968e65b8-f652-4c9f-bc58-98a97f2ba93a" xlink:to="loc_klic_ProgramAuthorized15August2017Member_14f40c65-dbc0-4739-b9a8-742462527897" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameAxis_b7bb51d4-63f4-45b2-b54d-2f3ceb9b06e4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PlanNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_76314a28-b0b0-489b-817a-57615a572ef3" xlink:to="loc_us-gaap_PlanNameAxis_b7bb51d4-63f4-45b2-b54d-2f3ceb9b06e4" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_b7bb51d4-63f4-45b2-b54d-2f3ceb9b06e4_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PlanNameAxis_b7bb51d4-63f4-45b2-b54d-2f3ceb9b06e4" xlink:to="loc_us-gaap_PlanNameDomain_b7bb51d4-63f4-45b2-b54d-2f3ceb9b06e4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_432b1896-cbca-45c9-a53b-a920fc611a81" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PlanNameAxis_b7bb51d4-63f4-45b2-b54d-2f3ceb9b06e4" xlink:to="loc_us-gaap_PlanNameDomain_432b1896-cbca-45c9-a53b-a920fc611a81" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_OmnibusIncentivePlanMember_8773e0ea-cf52-4c9c-964d-2c537feeaa37" xlink:href="klic-20240330.xsd#klic_OmnibusIncentivePlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_432b1896-cbca-45c9-a53b-a920fc611a81" xlink:to="loc_klic_OmnibusIncentivePlanMember_8773e0ea-cf52-4c9c-964d-2c537feeaa37" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_523fe9d7-ed7b-417d-b1cd-079b27d502e8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_76314a28-b0b0-489b-817a-57615a572ef3" xlink:to="loc_us-gaap_AwardTypeAxis_523fe9d7-ed7b-417d-b1cd-079b27d502e8" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_523fe9d7-ed7b-417d-b1cd-079b27d502e8_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_523fe9d7-ed7b-417d-b1cd-079b27d502e8" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_523fe9d7-ed7b-417d-b1cd-079b27d502e8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_d77242e2-c43d-48c1-bc0b-62b43a4e0ad0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_523fe9d7-ed7b-417d-b1cd-079b27d502e8" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_d77242e2-c43d-48c1-bc0b-62b43a4e0ad0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_RelativeTSRPerformanceShareUnitsMember_a8690cd4-d5a2-4ac7-b1d2-8cbe455c0715" xlink:href="klic-20240330.xsd#klic_RelativeTSRPerformanceShareUnitsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_d77242e2-c43d-48c1-bc0b-62b43a4e0ad0" xlink:to="loc_klic_RelativeTSRPerformanceShareUnitsMember_a8690cd4-d5a2-4ac7-b1d2-8cbe455c0715" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_RevenueGrowthPerformanceShareUnitsMember_6c77172c-b6f2-43ae-9008-4bd64e8db64e" xlink:href="klic-20240330.xsd#klic_RevenueGrowthPerformanceShareUnitsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_d77242e2-c43d-48c1-bc0b-62b43a4e0ad0" xlink:to="loc_klic_RevenueGrowthPerformanceShareUnitsMember_6c77172c-b6f2-43ae-9008-4bd64e8db64e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_TimeBasedRestrictedShareUnitsMember_a8187e41-1248-4710-93d3-570f963cc3df" xlink:href="klic-20240330.xsd#klic_TimeBasedRestrictedShareUnitsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_d77242e2-c43d-48c1-bc0b-62b43a4e0ad0" xlink:to="loc_klic_TimeBasedRestrictedShareUnitsMember_a8187e41-1248-4710-93d3-570f963cc3df" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementScenarioAxis_2d504879-0b38-4643-bf8d-d92f129e353e" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_StatementScenarioAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_76314a28-b0b0-489b-817a-57615a572ef3" xlink:to="loc_srt_StatementScenarioAxis_2d504879-0b38-4643-bf8d-d92f129e353e" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_2d504879-0b38-4643-bf8d-d92f129e353e_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementScenarioAxis_2d504879-0b38-4643-bf8d-d92f129e353e" xlink:to="loc_srt_ScenarioUnspecifiedDomain_2d504879-0b38-4643-bf8d-d92f129e353e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioUnspecifiedDomain_5b5cfd35-7a1c-4114-9c9a-982f4c71917f" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ScenarioUnspecifiedDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementScenarioAxis_2d504879-0b38-4643-bf8d-d92f129e353e" xlink:to="loc_srt_ScenarioUnspecifiedDomain_5b5cfd35-7a1c-4114-9c9a-982f4c71917f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ScenarioForecastMember_b2885287-0ad8-410b-84d4-34792fd1e9cf" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ScenarioForecastMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ScenarioUnspecifiedDomain_5b5cfd35-7a1c-4114-9c9a-982f4c71917f" xlink:to="loc_srt_ScenarioForecastMember_b2885287-0ad8-410b-84d4-34792fd1e9cf" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockRepurchaseProgramAuthorizedAmount1_bf6d559a-9471-43fa-a2d5-4c58c75e37de" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockRepurchaseProgramAuthorizedAmount1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_451a7206-9fb3-49ab-a9e7-bcd4246158d3" xlink:to="loc_us-gaap_StockRepurchaseProgramAuthorizedAmount1_bf6d559a-9471-43fa-a2d5-4c58c75e37de" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_StockRepurchaseProgramAuthorizedAmountAdditionalAmount_d3cb6ad0-0d14-4317-b02b-635babbfd728" xlink:href="klic-20240330.xsd#klic_StockRepurchaseProgramAuthorizedAmountAdditionalAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_451a7206-9fb3-49ab-a9e7-bcd4246158d3" xlink:to="loc_klic_StockRepurchaseProgramAuthorizedAmountAdditionalAmount_d3cb6ad0-0d14-4317-b02b-635babbfd728" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockValueAcquiredCostMethod_8676eae5-fc74-458a-a553-e527d7d02614" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TreasuryStockValueAcquiredCostMethod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_451a7206-9fb3-49ab-a9e7-bcd4246158d3" xlink:to="loc_us-gaap_TreasuryStockValueAcquiredCostMethod_8676eae5-fc74-458a-a553-e527d7d02614" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockSharesAcquired_1d144e19-308c-439b-8054-7300c6bc9c1d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TreasuryStockSharesAcquired"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_451a7206-9fb3-49ab-a9e7-bcd4246158d3" xlink:to="loc_us-gaap_TreasuryStockSharesAcquired_1d144e19-308c-439b-8054-7300c6bc9c1d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockRepurchaseProgramRemainingAuthorizedRepurchaseAmount1_eba01d1d-c0ec-4986-b164-1b257ff02ff7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockRepurchaseProgramRemainingAuthorizedRepurchaseAmount1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_451a7206-9fb3-49ab-a9e7-bcd4246158d3" xlink:to="loc_us-gaap_StockRepurchaseProgramRemainingAuthorizedRepurchaseAmount1_eba01d1d-c0ec-4986-b164-1b257ff02ff7" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockDividendsPerShareDeclared_66bc3cb0-162f-4d0a-a465-edbed29d589f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockDividendsPerShareDeclared"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_451a7206-9fb3-49ab-a9e7-bcd4246158d3" xlink:to="loc_us-gaap_CommonStockDividendsPerShareDeclared_66bc3cb0-162f-4d0a-a465-edbed29d589f" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsOfOrdinaryDividends_b373b5cc-39c9-4a90-bfad-64e0747d138e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PaymentsOfOrdinaryDividends"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_451a7206-9fb3-49ab-a9e7-bcd4246158d3" xlink:to="loc_us-gaap_PaymentsOfOrdinaryDividends_b373b5cc-39c9-4a90-bfad-64e0747d138e" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrant_51ec0048-43a7-40dc-a4ca-0a40b974773e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrant"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_451a7206-9fb3-49ab-a9e7-bcd4246158d3" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrant_51ec0048-43a7-40dc-a4ca-0a40b974773e" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_RelativeTotalShareholderReturnAverageStockPriceCalculationPeriod_d63c2e0c-4be9-4c0e-8591-82f13eb12f09" xlink:href="klic-20240330.xsd#klic_RelativeTotalShareholderReturnAverageStockPriceCalculationPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_451a7206-9fb3-49ab-a9e7-bcd4246158d3" xlink:to="loc_klic_RelativeTotalShareholderReturnAverageStockPriceCalculationPeriod_d63c2e0c-4be9-4c0e-8591-82f13eb12f09" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsVestingPeriod_172a4ead-efff-4915-b917-ce546a00b0c5" xlink:href="klic-20240330.xsd#klic_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsVestingPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_451a7206-9fb3-49ab-a9e7-bcd4246158d3" xlink:to="loc_klic_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsVestingPeriod_172a4ead-efff-4915-b917-ce546a00b0c5" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_ShareBasedCompensationArrangementByShareBasedPaymentAwardVestingPercentage_8740ad82-3cc8-4c00-be4b-636f121197e3" xlink:href="klic-20240330.xsd#klic_ShareBasedCompensationArrangementByShareBasedPaymentAwardVestingPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_451a7206-9fb3-49ab-a9e7-bcd4246158d3" xlink:to="loc_klic_ShareBasedCompensationArrangementByShareBasedPaymentAwardVestingPercentage_8740ad82-3cc8-4c00-be4b-636f121197e3" xlink:type="arc" order="10"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.kns.com/role/SHAREHOLDERSEQUITYANDEMPLOYEEBENEFITPLANSAccumulatedothercomprehensiveincomeDetails" xlink:type="simple" xlink:href="klic-20240330.xsd#SHAREHOLDERSEQUITYANDEMPLOYEEBENEFITPLANSAccumulatedothercomprehensiveincomeDetails"/>
  <link:definitionLink xlink:role="http://www.kns.com/role/SHAREHOLDERSEQUITYANDEMPLOYEEBENEFITPLANSAccumulatedothercomprehensiveincomeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_494f4484-94b1-41a5-95d6-8320b7f1d456" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_fdf1d187-1d50-483e-8db5-33525b150d31" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_494f4484-94b1-41a5-95d6-8320b7f1d456" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_fdf1d187-1d50-483e-8db5-33525b150d31" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_aafcd60e-be46-4d20-91c1-50cc6b82ad51" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_fdf1d187-1d50-483e-8db5-33525b150d31" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_aafcd60e-be46-4d20-91c1-50cc6b82ad51" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_aafcd60e-be46-4d20-91c1-50cc6b82ad51_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_aafcd60e-be46-4d20-91c1-50cc6b82ad51" xlink:to="loc_us-gaap_EquityComponentDomain_aafcd60e-be46-4d20-91c1-50cc6b82ad51_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_046158e4-251a-48c0-9aeb-d97035376f55" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_aafcd60e-be46-4d20-91c1-50cc6b82ad51" xlink:to="loc_us-gaap_EquityComponentDomain_046158e4-251a-48c0-9aeb-d97035376f55" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedTranslationAdjustmentMember_25f15326-061b-4a3a-99c0-5b219da05675" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccumulatedTranslationAdjustmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_046158e4-251a-48c0-9aeb-d97035376f55" xlink:to="loc_us-gaap_AccumulatedTranslationAdjustmentMember_25f15326-061b-4a3a-99c0-5b219da05675" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDefinedBenefitPlansAdjustmentMember_4ef83c2a-7110-4216-a164-627f32e12761" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccumulatedDefinedBenefitPlansAdjustmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_046158e4-251a-48c0-9aeb-d97035376f55" xlink:to="loc_us-gaap_AccumulatedDefinedBenefitPlansAdjustmentMember_4ef83c2a-7110-4216-a164-627f32e12761" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedGainLossNetCashFlowHedgeParentMember_31f00e8e-8db8-4a9a-9f97-b77ea075f08b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccumulatedGainLossNetCashFlowHedgeParentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_046158e4-251a-48c0-9aeb-d97035376f55" xlink:to="loc_us-gaap_AccumulatedGainLossNetCashFlowHedgeParentMember_31f00e8e-8db8-4a9a-9f97-b77ea075f08b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_a973a9a6-5a71-4572-83f3-2da99f7f01dd" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_046158e4-251a-48c0-9aeb-d97035376f55" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_a973a9a6-5a71-4572-83f3-2da99f7f01dd" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_7a579abc-c88a-4b03-b3a4-bf991b2c669c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_494f4484-94b1-41a5-95d6-8320b7f1d456" xlink:to="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_7a579abc-c88a-4b03-b3a4-bf991b2c669c" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.kns.com/role/SHAREHOLDERSEQUITYANDEMPLOYEEBENEFITPLANSRestrictedstockandcommonstockgrantedDetails" xlink:type="simple" xlink:href="klic-20240330.xsd#SHAREHOLDERSEQUITYANDEMPLOYEEBENEFITPLANSRestrictedstockandcommonstockgrantedDetails"/>
  <link:definitionLink xlink:role="http://www.kns.com/role/SHAREHOLDERSEQUITYANDEMPLOYEEBENEFITPLANSRestrictedstockandcommonstockgrantedDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_aef7c81b-ab57-4de7-8a3d-1ec90b855360" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_4cb0882f-15cd-4581-957c-6d5433e07ee2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_aef7c81b-ab57-4de7-8a3d-1ec90b855360" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_4cb0882f-15cd-4581-957c-6d5433e07ee2" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_a3059b6f-f5bf-4419-bb64-0d2994782f02" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_4cb0882f-15cd-4581-957c-6d5433e07ee2" xlink:to="loc_us-gaap_AwardTypeAxis_a3059b6f-f5bf-4419-bb64-0d2994782f02" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_a3059b6f-f5bf-4419-bb64-0d2994782f02_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_a3059b6f-f5bf-4419-bb64-0d2994782f02" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_a3059b6f-f5bf-4419-bb64-0d2994782f02_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_8adac93a-22b9-464f-a80f-5237c3aa48fe" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_a3059b6f-f5bf-4419-bb64-0d2994782f02" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_8adac93a-22b9-464f-a80f-5237c3aa48fe" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_TimebasedRSUMember_5c70d42e-c23d-442d-bfef-391a18e2057a" xlink:href="klic-20240330.xsd#klic_TimebasedRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_8adac93a-22b9-464f-a80f-5237c3aa48fe" xlink:to="loc_klic_TimebasedRSUMember_5c70d42e-c23d-442d-bfef-391a18e2057a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_RelativeTSRPSUMember_c46f1bea-3d29-41c4-9526-37b8ecaebcd4" xlink:href="klic-20240330.xsd#klic_RelativeTSRPSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_8adac93a-22b9-464f-a80f-5237c3aa48fe" xlink:to="loc_klic_RelativeTSRPSUMember_c46f1bea-3d29-41c4-9526-37b8ecaebcd4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_SpecialGrowthPSUMember_0b380137-f8cc-4f3b-bfa3-11165c55ba23" xlink:href="klic-20240330.xsd#klic_SpecialGrowthPSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_8adac93a-22b9-464f-a80f-5237c3aa48fe" xlink:to="loc_klic_SpecialGrowthPSUMember_0b380137-f8cc-4f3b-bfa3-11165c55ba23" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_6ce62587-3a49-4906-a412-96a2ab3d6e52" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_8adac93a-22b9-464f-a80f-5237c3aa48fe" xlink:to="loc_us-gaap_CommonStockMember_6ce62587-3a49-4906-a412-96a2ab3d6e52" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardSharesIssuedInPeriod_921877a9-9b37-4139-8772-fc471e658163" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardSharesIssuedInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_aef7c81b-ab57-4de7-8a3d-1ec90b855360" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardSharesIssuedInPeriod_921877a9-9b37-4139-8772-fc471e658163" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.kns.com/role/SHAREHOLDERSEQUITYANDEMPLOYEEBENEFITPLANSTotalequitybasedcompensationexpenseDetails" xlink:type="simple" xlink:href="klic-20240330.xsd#SHAREHOLDERSEQUITYANDEMPLOYEEBENEFITPLANSTotalequitybasedcompensationexpenseDetails"/>
  <link:definitionLink xlink:role="http://www.kns.com/role/SHAREHOLDERSEQUITYANDEMPLOYEEBENEFITPLANSTotalequitybasedcompensationexpenseDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_2d992cb8-b0f4-4802-9bc6-73cd99b9cc44" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfEmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsTable_1b357984-76ed-4ea1-8ec7-e268fba7de8a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfEmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_2d992cb8-b0f4-4802-9bc6-73cd99b9cc44" xlink:to="loc_us-gaap_ScheduleOfEmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsTable_1b357984-76ed-4ea1-8ec7-e268fba7de8a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationAxis_0095fe90-ccf0-4435-9607-ae4d37acd115" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeStatementLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfEmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsTable_1b357984-76ed-4ea1-8ec7-e268fba7de8a" xlink:to="loc_us-gaap_IncomeStatementLocationAxis_0095fe90-ccf0-4435-9607-ae4d37acd115" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_0095fe90-ccf0-4435-9607-ae4d37acd115_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_0095fe90-ccf0-4435-9607-ae4d37acd115" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_0095fe90-ccf0-4435-9607-ae4d37acd115_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_146e0173-c263-4910-b8b9-ea7a8a86974d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_0095fe90-ccf0-4435-9607-ae4d37acd115" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_146e0173-c263-4910-b8b9-ea7a8a86974d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfSalesMember_bb446dc8-54c6-4915-b5ba-1a840449fdf4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CostOfSalesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_146e0173-c263-4910-b8b9-ea7a8a86974d" xlink:to="loc_us-gaap_CostOfSalesMember_bb446dc8-54c6-4915-b5ba-1a840449fdf4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingGeneralAndAdministrativeExpensesMember_aba779c7-4cec-4b94-9cfb-6947e587bbbe" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SellingGeneralAndAdministrativeExpensesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_146e0173-c263-4910-b8b9-ea7a8a86974d" xlink:to="loc_us-gaap_SellingGeneralAndAdministrativeExpensesMember_aba779c7-4cec-4b94-9cfb-6947e587bbbe" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpenseMember_ec5032b9-1d2f-4cf6-a443-55f6cdf191e0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ResearchAndDevelopmentExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_146e0173-c263-4910-b8b9-ea7a8a86974d" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpenseMember_ec5032b9-1d2f-4cf6-a443-55f6cdf191e0" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_f908123c-6792-43f9-8d62-8f20473cdcb1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfEmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsTable_1b357984-76ed-4ea1-8ec7-e268fba7de8a" xlink:to="loc_us-gaap_AwardTypeAxis_f908123c-6792-43f9-8d62-8f20473cdcb1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_f908123c-6792-43f9-8d62-8f20473cdcb1_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_f908123c-6792-43f9-8d62-8f20473cdcb1" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_f908123c-6792-43f9-8d62-8f20473cdcb1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_d2f76c75-8257-4520-b13f-890c1117b0d3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_f908123c-6792-43f9-8d62-8f20473cdcb1" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_d2f76c75-8257-4520-b13f-890c1117b0d3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_TimebasedRSUMember_65c3273a-37bd-475a-a96d-466b9b69cfbb" xlink:href="klic-20240330.xsd#klic_TimebasedRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_d2f76c75-8257-4520-b13f-890c1117b0d3" xlink:to="loc_klic_TimebasedRSUMember_65c3273a-37bd-475a-a96d-466b9b69cfbb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_RelativeTSRPSUMember_336a9c84-ba8b-432a-83c8-945720a52b7d" xlink:href="klic-20240330.xsd#klic_RelativeTSRPSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_d2f76c75-8257-4520-b13f-890c1117b0d3" xlink:to="loc_klic_RelativeTSRPSUMember_336a9c84-ba8b-432a-83c8-945720a52b7d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_SpecialGrowthPSUMember_10e60ee2-ad32-45ba-822c-da7239426983" xlink:href="klic-20240330.xsd#klic_SpecialGrowthPSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_d2f76c75-8257-4520-b13f-890c1117b0d3" xlink:to="loc_klic_SpecialGrowthPSUMember_10e60ee2-ad32-45ba-822c-da7239426983" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_231e202e-b8a5-4d80-b108-4c9c97f7c8de" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_d2f76c75-8257-4520-b13f-890c1117b0d3" xlink:to="loc_us-gaap_CommonStockMember_231e202e-b8a5-4d80-b108-4c9c97f7c8de" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllocatedShareBasedCompensationExpense_491080a6-c6bb-444a-a7f6-8de0c5b66a44" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AllocatedShareBasedCompensationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_2d992cb8-b0f4-4802-9bc6-73cd99b9cc44" xlink:to="loc_us-gaap_AllocatedShareBasedCompensationExpense_491080a6-c6bb-444a-a7f6-8de0c5b66a44" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.kns.com/role/SEGMENTINFORMATIONOperatinginformationbysegmentDetails" xlink:type="simple" xlink:href="klic-20240330.xsd#SEGMENTINFORMATIONOperatinginformationbysegmentDetails"/>
  <link:definitionLink xlink:role="http://www.kns.com/role/SEGMENTINFORMATIONOperatinginformationbysegmentDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentReportingRevenueReconcilingItemLineItems_0e13a712-27b1-45d9-aeee-e470c064d9cf" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentReportingRevenueReconcilingItemLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReconciliationOfRevenueFromSegmentsToConsolidatedTable_95f5820d-521b-47e7-88ef-0c5f78afa91c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ReconciliationOfRevenueFromSegmentsToConsolidatedTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SegmentReportingRevenueReconcilingItemLineItems_0e13a712-27b1-45d9-aeee-e470c064d9cf" xlink:to="loc_us-gaap_ReconciliationOfRevenueFromSegmentsToConsolidatedTable_95f5820d-521b-47e7-88ef-0c5f78afa91c" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_3cec29f4-862d-401e-915c-d067a7baf8f6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ReconciliationOfRevenueFromSegmentsToConsolidatedTable_95f5820d-521b-47e7-88ef-0c5f78afa91c" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_3cec29f4-862d-401e-915c-d067a7baf8f6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_3cec29f4-862d-401e-915c-d067a7baf8f6_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_3cec29f4-862d-401e-915c-d067a7baf8f6" xlink:to="loc_us-gaap_SegmentDomain_3cec29f4-862d-401e-915c-d067a7baf8f6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_b51da37b-3d53-4116-b770-bd5aa781b322" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_3cec29f4-862d-401e-915c-d067a7baf8f6" xlink:to="loc_us-gaap_SegmentDomain_b51da37b-3d53-4116-b770-bd5aa781b322" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_BallBondingEquipmentSegmentMember_b5c3f791-3fda-402d-b34b-2a98fe2a3970" xlink:href="klic-20240330.xsd#klic_BallBondingEquipmentSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_b51da37b-3d53-4116-b770-bd5aa781b322" xlink:to="loc_klic_BallBondingEquipmentSegmentMember_b5c3f791-3fda-402d-b34b-2a98fe2a3970" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_WedgeBondingEquipmentSegmentMember_0fb41b77-a314-42a8-8a41-19154a577d3c" xlink:href="klic-20240330.xsd#klic_WedgeBondingEquipmentSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_b51da37b-3d53-4116-b770-bd5aa781b322" xlink:to="loc_klic_WedgeBondingEquipmentSegmentMember_0fb41b77-a314-42a8-8a41-19154a577d3c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_AdvancedSolutionsSegmentMember_891231b3-d1fb-4487-a754-e528507ba837" xlink:href="klic-20240330.xsd#klic_AdvancedSolutionsSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_b51da37b-3d53-4116-b770-bd5aa781b322" xlink:to="loc_klic_AdvancedSolutionsSegmentMember_891231b3-d1fb-4487-a754-e528507ba837" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_AftermarketProductsandServicesAPSSegmentMember_5d5acb95-cd39-4fd2-a0b0-9c2acd29e69c" xlink:href="klic-20240330.xsd#klic_AftermarketProductsandServicesAPSSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_b51da37b-3d53-4116-b770-bd5aa781b322" xlink:to="loc_klic_AftermarketProductsandServicesAPSSegmentMember_5d5acb95-cd39-4fd2-a0b0-9c2acd29e69c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_AllOthersSegmentMember_65eccdc7-294f-4c78-afe4-f5038841eb24" xlink:href="klic-20240330.xsd#klic_AllOthersSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_b51da37b-3d53-4116-b770-bd5aa781b322" xlink:to="loc_klic_AllOthersSegmentMember_65eccdc7-294f-4c78-afe4-f5038841eb24" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_CorporateExpensesMember_9db1e118-a267-4e4b-bdf9-9d631b097e8c" xlink:href="klic-20240330.xsd#klic_CorporateExpensesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_b51da37b-3d53-4116-b770-bd5aa781b322" xlink:to="loc_klic_CorporateExpensesMember_9db1e118-a267-4e4b-bdf9-9d631b097e8c" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenuesAbstract_61b34721-bc9c-4ba0-b364-efcf30558d47" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RevenuesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingRevenueReconcilingItemLineItems_0e13a712-27b1-45d9-aeee-e470c064d9cf" xlink:to="loc_us-gaap_RevenuesAbstract_61b34721-bc9c-4ba0-b364-efcf30558d47" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Revenues_deafe3bb-de4c-46ed-88eb-5340c6264826" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_Revenues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenuesAbstract_61b34721-bc9c-4ba0-b364-efcf30558d47" xlink:to="loc_us-gaap_Revenues_deafe3bb-de4c-46ed-88eb-5340c6264826" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLossAbstract_146868fb-9243-494e-bd46-6e91599ea8a3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OperatingIncomeLossAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingRevenueReconcilingItemLineItems_0e13a712-27b1-45d9-aeee-e470c064d9cf" xlink:to="loc_us-gaap_OperatingIncomeLossAbstract_146868fb-9243-494e-bd46-6e91599ea8a3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_7f2379be-73ba-4c4a-837a-ee3195708f37" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingIncomeLossAbstract_146868fb-9243-494e-bd46-6e91599ea8a3" xlink:to="loc_us-gaap_OperatingIncomeLoss_7f2379be-73ba-4c4a-837a-ee3195708f37" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.kns.com/role/SEGMENTINFORMATIONScheduleofnetrevenuebyCapitalEquipmentendmarketsDetails" xlink:type="simple" xlink:href="klic-20240330.xsd#SEGMENTINFORMATIONScheduleofnetrevenuebyCapitalEquipmentendmarketsDetails"/>
  <link:definitionLink xlink:role="http://www.kns.com/role/SEGMENTINFORMATIONScheduleofnetrevenuebyCapitalEquipmentendmarketsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentReportingRevenueReconcilingItemLineItems_1e14974c-8d9e-4d2c-99c0-65178a988526" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentReportingRevenueReconcilingItemLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReconciliationOfRevenueFromSegmentsToConsolidatedTable_dcb2a02e-b732-4004-9274-a566bd0847e4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ReconciliationOfRevenueFromSegmentsToConsolidatedTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SegmentReportingRevenueReconcilingItemLineItems_1e14974c-8d9e-4d2c-99c0-65178a988526" xlink:to="loc_us-gaap_ReconciliationOfRevenueFromSegmentsToConsolidatedTable_dcb2a02e-b732-4004-9274-a566bd0847e4" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_5e576cc0-b3f2-4ee0-b0e6-caa2c0ba2a09" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ReconciliationOfRevenueFromSegmentsToConsolidatedTable_dcb2a02e-b732-4004-9274-a566bd0847e4" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_5e576cc0-b3f2-4ee0-b0e6-caa2c0ba2a09" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_5e576cc0-b3f2-4ee0-b0e6-caa2c0ba2a09_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_5e576cc0-b3f2-4ee0-b0e6-caa2c0ba2a09" xlink:to="loc_us-gaap_SegmentDomain_5e576cc0-b3f2-4ee0-b0e6-caa2c0ba2a09_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_c0e5a266-9ea7-4e23-ba3d-fd31e27225ab" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_5e576cc0-b3f2-4ee0-b0e6-caa2c0ba2a09" xlink:to="loc_us-gaap_SegmentDomain_c0e5a266-9ea7-4e23-ba3d-fd31e27225ab" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_AllSegmentsExcludingAftermarketProductsAndServicesAPSMember_b328d490-e3d8-4baa-a4c8-292ae0c6837d" xlink:href="klic-20240330.xsd#klic_AllSegmentsExcludingAftermarketProductsAndServicesAPSMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_c0e5a266-9ea7-4e23-ba3d-fd31e27225ab" xlink:to="loc_klic_AllSegmentsExcludingAftermarketProductsAndServicesAPSMember_b328d490-e3d8-4baa-a4c8-292ae0c6837d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_AftermarketProductsandServicesAPSSegmentMember_8fd3e1b4-7ae8-4367-9c44-428da90afd5f" xlink:href="klic-20240330.xsd#klic_AftermarketProductsandServicesAPSSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_c0e5a266-9ea7-4e23-ba3d-fd31e27225ab" xlink:to="loc_klic_AftermarketProductsandServicesAPSSegmentMember_8fd3e1b4-7ae8-4367-9c44-428da90afd5f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_daaf1661-a3f8-4877-a32a-578d09404f19" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ReconciliationOfRevenueFromSegmentsToConsolidatedTable_dcb2a02e-b732-4004-9274-a566bd0847e4" xlink:to="loc_srt_ProductOrServiceAxis_daaf1661-a3f8-4877-a32a-578d09404f19" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_daaf1661-a3f8-4877-a32a-578d09404f19_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_daaf1661-a3f8-4877-a32a-578d09404f19" xlink:to="loc_srt_ProductsAndServicesDomain_daaf1661-a3f8-4877-a32a-578d09404f19_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_3c112844-e6b8-46e1-9369-b4167bb6a100" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_daaf1661-a3f8-4877-a32a-578d09404f19" xlink:to="loc_srt_ProductsAndServicesDomain_3c112844-e6b8-46e1-9369-b4167bb6a100" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_GeneralSemiconductorMember_6b157613-2870-4a15-b6b6-8a5331f9628f" xlink:href="klic-20240330.xsd#klic_GeneralSemiconductorMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_3c112844-e6b8-46e1-9369-b4167bb6a100" xlink:to="loc_klic_GeneralSemiconductorMember_6b157613-2870-4a15-b6b6-8a5331f9628f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_AutomotiveandIndustrialMember_342f5940-5244-4ac5-bf12-abd34615ccda" xlink:href="klic-20240330.xsd#klic_AutomotiveandIndustrialMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_3c112844-e6b8-46e1-9369-b4167bb6a100" xlink:to="loc_klic_AutomotiveandIndustrialMember_342f5940-5244-4ac5-bf12-abd34615ccda" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_LEDMember_8da93d63-7951-48ce-8dc2-450cb3ea9ef3" xlink:href="klic-20240330.xsd#klic_LEDMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_3c112844-e6b8-46e1-9369-b4167bb6a100" xlink:to="loc_klic_LEDMember_8da93d63-7951-48ce-8dc2-450cb3ea9ef3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_MemoryMember_3882f4d3-4571-4516-932f-c255681b0889" xlink:href="klic-20240330.xsd#klic_MemoryMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_3c112844-e6b8-46e1-9369-b4167bb6a100" xlink:to="loc_klic_MemoryMember_3882f4d3-4571-4516-932f-c255681b0889" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Revenues_df588a55-5413-439b-84cd-f43a944d8b2e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_Revenues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingRevenueReconcilingItemLineItems_1e14974c-8d9e-4d2c-99c0-65178a988526" xlink:to="loc_us-gaap_Revenues_df588a55-5413-439b-84cd-f43a944d8b2e" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.kns.com/role/SEGMENTINFORMATIONCapitalexpendituresdepreciationandamortizationexpensebysegmentDetails" xlink:type="simple" xlink:href="klic-20240330.xsd#SEGMENTINFORMATIONCapitalexpendituresdepreciationandamortizationexpensebysegmentDetails"/>
  <link:definitionLink xlink:role="http://www.kns.com/role/SEGMENTINFORMATIONCapitalexpendituresdepreciationandamortizationexpensebysegmentDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentReportingOtherSignificantReconcilingItemLineItems_381ba756-f1e0-40f1-b2f7-27a1b89bfc68" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentReportingOtherSignificantReconcilingItemLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReconciliationOfOtherSignificantReconcilingItemsFromSegmentsToConsolidatedTable_6d1b0c60-44ba-464f-8333-1eedbb585ddf" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ReconciliationOfOtherSignificantReconcilingItemsFromSegmentsToConsolidatedTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SegmentReportingOtherSignificantReconcilingItemLineItems_381ba756-f1e0-40f1-b2f7-27a1b89bfc68" xlink:to="loc_us-gaap_ReconciliationOfOtherSignificantReconcilingItemsFromSegmentsToConsolidatedTable_6d1b0c60-44ba-464f-8333-1eedbb585ddf" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_c0bdd125-6f8d-4371-b2ca-6488d0994d8a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ReconciliationOfOtherSignificantReconcilingItemsFromSegmentsToConsolidatedTable_6d1b0c60-44ba-464f-8333-1eedbb585ddf" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_c0bdd125-6f8d-4371-b2ca-6488d0994d8a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_c0bdd125-6f8d-4371-b2ca-6488d0994d8a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_c0bdd125-6f8d-4371-b2ca-6488d0994d8a" xlink:to="loc_us-gaap_SegmentDomain_c0bdd125-6f8d-4371-b2ca-6488d0994d8a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_64d26c57-fc8e-4580-91cc-6e0453769fcd" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_c0bdd125-6f8d-4371-b2ca-6488d0994d8a" xlink:to="loc_us-gaap_SegmentDomain_64d26c57-fc8e-4580-91cc-6e0453769fcd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_BallBondingEquipmentSegmentMember_251fa688-bf60-4c6d-9236-f0e8b4395437" xlink:href="klic-20240330.xsd#klic_BallBondingEquipmentSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_64d26c57-fc8e-4580-91cc-6e0453769fcd" xlink:to="loc_klic_BallBondingEquipmentSegmentMember_251fa688-bf60-4c6d-9236-f0e8b4395437" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_WedgeBondingEquipmentSegmentMember_48cfb20a-41c3-40a7-83cc-8133e29a5c8b" xlink:href="klic-20240330.xsd#klic_WedgeBondingEquipmentSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_64d26c57-fc8e-4580-91cc-6e0453769fcd" xlink:to="loc_klic_WedgeBondingEquipmentSegmentMember_48cfb20a-41c3-40a7-83cc-8133e29a5c8b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_AdvancedSolutionsSegmentMember_3687f6ab-c6a9-43f1-ac89-cefd35ea613d" xlink:href="klic-20240330.xsd#klic_AdvancedSolutionsSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_64d26c57-fc8e-4580-91cc-6e0453769fcd" xlink:to="loc_klic_AdvancedSolutionsSegmentMember_3687f6ab-c6a9-43f1-ac89-cefd35ea613d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_AftermarketProductsandServicesAPSSegmentMember_7d97701a-b9f0-44f0-a933-1aa68161fa05" xlink:href="klic-20240330.xsd#klic_AftermarketProductsandServicesAPSSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_64d26c57-fc8e-4580-91cc-6e0453769fcd" xlink:to="loc_klic_AftermarketProductsandServicesAPSSegmentMember_7d97701a-b9f0-44f0-a933-1aa68161fa05" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_AllOthersSegmentMember_0e60d96e-f6ed-4a21-a923-80677ad7fe69" xlink:href="klic-20240330.xsd#klic_AllOthersSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_64d26c57-fc8e-4580-91cc-6e0453769fcd" xlink:to="loc_klic_AllOthersSegmentMember_0e60d96e-f6ed-4a21-a923-80677ad7fe69" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_CorporateExpensesMember_483b76f4-8a62-4613-b83b-87d5f97b8ced" xlink:href="klic-20240330.xsd#klic_CorporateExpensesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_64d26c57-fc8e-4580-91cc-6e0453769fcd" xlink:to="loc_klic_CorporateExpensesMember_483b76f4-8a62-4613-b83b-87d5f97b8ced" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentAdditions_40343761-398c-48c5-852c-0416c27e2b2b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentAdditions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingOtherSignificantReconcilingItemLineItems_381ba756-f1e0-40f1-b2f7-27a1b89bfc68" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentAdditions_40343761-398c-48c5-852c-0416c27e2b2b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepreciationAbstract_79db808e-ca81-4ce4-ac75-511bd86bf461" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DepreciationAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingOtherSignificantReconcilingItemLineItems_381ba756-f1e0-40f1-b2f7-27a1b89bfc68" xlink:to="loc_us-gaap_DepreciationAbstract_79db808e-ca81-4ce4-ac75-511bd86bf461" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Depreciation_7fc981ad-7581-434a-94fa-b504cb6983c8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_Depreciation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DepreciationAbstract_79db808e-ca81-4ce4-ac75-511bd86bf461" xlink:to="loc_us-gaap_Depreciation_7fc981ad-7581-434a-94fa-b504cb6983c8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AmortizationOfIntangibleAssets_89de8d70-aa65-44b6-98cb-fd7fcda6242f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AmortizationOfIntangibleAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DepreciationAbstract_79db808e-ca81-4ce4-ac75-511bd86bf461" xlink:to="loc_us-gaap_AmortizationOfIntangibleAssets_89de8d70-aa65-44b6-98cb-fd7fcda6242f" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.kns.com/role/COMMITMENTSCONTINGENCIESANDCONCENTRATIONSSignificantcustomerconcentrationsasapercentageofnetrevenueDetails" xlink:type="simple" xlink:href="klic-20240330.xsd#COMMITMENTSCONTINGENCIESANDCONCENTRATIONSSignificantcustomerconcentrationsasapercentageofnetrevenueDetails"/>
  <link:definitionLink xlink:role="http://www.kns.com/role/COMMITMENTSCONTINGENCIESANDCONCENTRATIONSSignificantcustomerconcentrationsasapercentageofnetrevenueDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskLineItems_c78e1cbd-ebf7-4d1f-90a3-a893068915ab" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTable_99f68702-6468-46dc-a5e9-2b9e2cc8144f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ConcentrationRiskLineItems_c78e1cbd-ebf7-4d1f-90a3-a893068915ab" xlink:to="loc_us-gaap_ConcentrationRiskTable_99f68702-6468-46dc-a5e9-2b9e2cc8144f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_CustomerAxis_3e934ee7-c60e-4273-83f9-0a120839a69b" xlink:href="klic-20240330.xsd#klic_CustomerAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ConcentrationRiskTable_99f68702-6468-46dc-a5e9-2b9e2cc8144f" xlink:to="loc_klic_CustomerAxis_3e934ee7-c60e-4273-83f9-0a120839a69b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_CustomerDomain_3e934ee7-c60e-4273-83f9-0a120839a69b_default" xlink:href="klic-20240330.xsd#klic_CustomerDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_klic_CustomerAxis_3e934ee7-c60e-4273-83f9-0a120839a69b" xlink:to="loc_klic_CustomerDomain_3e934ee7-c60e-4273-83f9-0a120839a69b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_CustomerDomain_e64314e7-65bf-4531-b1c2-1e04171e5abd" xlink:href="klic-20240330.xsd#klic_CustomerDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_klic_CustomerAxis_3e934ee7-c60e-4273-83f9-0a120839a69b" xlink:to="loc_klic_CustomerDomain_e64314e7-65bf-4531-b1c2-1e04171e5abd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_FirstTechnologyChinaLtdMember_9d5252b6-46f1-409c-b0c1-ed7a143db707" xlink:href="klic-20240330.xsd#klic_FirstTechnologyChinaLtdMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_klic_CustomerDomain_e64314e7-65bf-4531-b1c2-1e04171e5abd" xlink:to="loc_klic_FirstTechnologyChinaLtdMember_9d5252b6-46f1-409c-b0c1-ed7a143db707" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_MatfronShanghaiMember_9d01131a-bf90-4ed6-a6bb-5ea37869f964" xlink:href="klic-20240330.xsd#klic_MatfronShanghaiMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_klic_CustomerDomain_e64314e7-65bf-4531-b1c2-1e04171e5abd" xlink:to="loc_klic_MatfronShanghaiMember_9d01131a-bf90-4ed6-a6bb-5ea37869f964" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_25289103-57d9-4deb-bdb0-258669880104" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskByBenchmarkAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ConcentrationRiskTable_99f68702-6468-46dc-a5e9-2b9e2cc8144f" xlink:to="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_25289103-57d9-4deb-bdb0-258669880104" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_25289103-57d9-4deb-bdb0-258669880104_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_25289103-57d9-4deb-bdb0-258669880104" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_25289103-57d9-4deb-bdb0-258669880104_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_460ade69-ddc7-4f6f-bff8-079e27ea37ed" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_25289103-57d9-4deb-bdb0-258669880104" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_460ade69-ddc7-4f6f-bff8-079e27ea37ed" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SalesRevenueNetMember_3e479c97-7ad1-451e-ad2e-98a67d87e623" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SalesRevenueNetMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskBenchmarkDomain_460ade69-ddc7-4f6f-bff8-079e27ea37ed" xlink:to="loc_us-gaap_SalesRevenueNetMember_3e479c97-7ad1-451e-ad2e-98a67d87e623" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByTypeAxis_21dfd7ac-a32d-4947-9e33-d54c49e5cb7d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ConcentrationRiskTable_99f68702-6468-46dc-a5e9-2b9e2cc8144f" xlink:to="loc_us-gaap_ConcentrationRiskByTypeAxis_21dfd7ac-a32d-4947-9e33-d54c49e5cb7d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTypeDomain_21dfd7ac-a32d-4947-9e33-d54c49e5cb7d_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByTypeAxis_21dfd7ac-a32d-4947-9e33-d54c49e5cb7d" xlink:to="loc_us-gaap_ConcentrationRiskTypeDomain_21dfd7ac-a32d-4947-9e33-d54c49e5cb7d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTypeDomain_1d71eb58-1b0e-44cb-b44e-21accc059574" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByTypeAxis_21dfd7ac-a32d-4947-9e33-d54c49e5cb7d" xlink:to="loc_us-gaap_ConcentrationRiskTypeDomain_1d71eb58-1b0e-44cb-b44e-21accc059574" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CustomerConcentrationRiskMember_7df6c729-a352-46f0-8e73-9bfc0026f01f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CustomerConcentrationRiskMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskTypeDomain_1d71eb58-1b0e-44cb-b44e-21accc059574" xlink:to="loc_us-gaap_CustomerConcentrationRiskMember_7df6c729-a352-46f0-8e73-9bfc0026f01f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskPercentage1_449beda1-c62e-46c6-bc00-a2e9809a77cc" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskPercentage1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskLineItems_c78e1cbd-ebf7-4d1f-90a3-a893068915ab" xlink:to="loc_us-gaap_ConcentrationRiskPercentage1_449beda1-c62e-46c6-bc00-a2e9809a77cc" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.kns.com/role/COMMITMENTSCONTINGENCIESANDCONCENTRATIONSSignificantcustomerconcentrationsasapercentageoftotalaccountsreceivableDetails" xlink:type="simple" xlink:href="klic-20240330.xsd#COMMITMENTSCONTINGENCIESANDCONCENTRATIONSSignificantcustomerconcentrationsasapercentageoftotalaccountsreceivableDetails"/>
  <link:definitionLink xlink:role="http://www.kns.com/role/COMMITMENTSCONTINGENCIESANDCONCENTRATIONSSignificantcustomerconcentrationsasapercentageoftotalaccountsreceivableDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskLineItems_e54802ae-4817-4e0c-ac42-391ad451f62b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTable_aecee313-7301-495a-8ab5-a42df3afd01e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ConcentrationRiskLineItems_e54802ae-4817-4e0c-ac42-391ad451f62b" xlink:to="loc_us-gaap_ConcentrationRiskTable_aecee313-7301-495a-8ab5-a42df3afd01e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_CustomerAxis_54647168-74ff-4d40-bcf8-43cd51954216" xlink:href="klic-20240330.xsd#klic_CustomerAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ConcentrationRiskTable_aecee313-7301-495a-8ab5-a42df3afd01e" xlink:to="loc_klic_CustomerAxis_54647168-74ff-4d40-bcf8-43cd51954216" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_CustomerDomain_54647168-74ff-4d40-bcf8-43cd51954216_default" xlink:href="klic-20240330.xsd#klic_CustomerDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_klic_CustomerAxis_54647168-74ff-4d40-bcf8-43cd51954216" xlink:to="loc_klic_CustomerDomain_54647168-74ff-4d40-bcf8-43cd51954216_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_CustomerDomain_ea799fb9-e017-4ba0-849f-e58cbfda47a3" xlink:href="klic-20240330.xsd#klic_CustomerDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_klic_CustomerAxis_54647168-74ff-4d40-bcf8-43cd51954216" xlink:to="loc_klic_CustomerDomain_ea799fb9-e017-4ba0-849f-e58cbfda47a3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_IntelCorporationMember_3255169a-c569-49e0-b77a-27e7c5663795" xlink:href="klic-20240330.xsd#klic_IntelCorporationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_klic_CustomerDomain_ea799fb9-e017-4ba0-849f-e58cbfda47a3" xlink:to="loc_klic_IntelCorporationMember_3255169a-c569-49e0-b77a-27e7c5663795" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_ForehopeElectronicMember_f5b0a496-4762-4b7b-ac89-3cf0a2d146fe" xlink:href="klic-20240330.xsd#klic_ForehopeElectronicMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_klic_CustomerDomain_ea799fb9-e017-4ba0-849f-e58cbfda47a3" xlink:to="loc_klic_ForehopeElectronicMember_f5b0a496-4762-4b7b-ac89-3cf0a2d146fe" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_HuatianTechnologyGroupMember_5a6891d5-ba2c-4319-84a1-0aa1d7d30b4e" xlink:href="klic-20240330.xsd#klic_HuatianTechnologyGroupMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_klic_CustomerDomain_ea799fb9-e017-4ba0-849f-e58cbfda47a3" xlink:to="loc_klic_HuatianTechnologyGroupMember_5a6891d5-ba2c-4319-84a1-0aa1d7d30b4e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_2f0f9004-22d1-4c0e-8f5c-fcb256d9bbc0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskByBenchmarkAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ConcentrationRiskTable_aecee313-7301-495a-8ab5-a42df3afd01e" xlink:to="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_2f0f9004-22d1-4c0e-8f5c-fcb256d9bbc0" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_2f0f9004-22d1-4c0e-8f5c-fcb256d9bbc0_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_2f0f9004-22d1-4c0e-8f5c-fcb256d9bbc0" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_2f0f9004-22d1-4c0e-8f5c-fcb256d9bbc0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_5575e23c-67c7-4112-b00a-2905613939d1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_2f0f9004-22d1-4c0e-8f5c-fcb256d9bbc0" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_5575e23c-67c7-4112-b00a-2905613939d1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsReceivableMember_57315204-373d-4671-8d2d-b3f6bbe72992" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccountsReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskBenchmarkDomain_5575e23c-67c7-4112-b00a-2905613939d1" xlink:to="loc_us-gaap_AccountsReceivableMember_57315204-373d-4671-8d2d-b3f6bbe72992" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByTypeAxis_d20d4bbf-e185-4e8c-ae14-65d328389bcb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ConcentrationRiskTable_aecee313-7301-495a-8ab5-a42df3afd01e" xlink:to="loc_us-gaap_ConcentrationRiskByTypeAxis_d20d4bbf-e185-4e8c-ae14-65d328389bcb" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTypeDomain_d20d4bbf-e185-4e8c-ae14-65d328389bcb_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByTypeAxis_d20d4bbf-e185-4e8c-ae14-65d328389bcb" xlink:to="loc_us-gaap_ConcentrationRiskTypeDomain_d20d4bbf-e185-4e8c-ae14-65d328389bcb_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTypeDomain_787cbd76-b285-4b50-b1a7-1c971d3f2728" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByTypeAxis_d20d4bbf-e185-4e8c-ae14-65d328389bcb" xlink:to="loc_us-gaap_ConcentrationRiskTypeDomain_787cbd76-b285-4b50-b1a7-1c971d3f2728" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CustomerConcentrationRiskMember_5296a623-b6a3-472c-b302-fc34f40df0d2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CustomerConcentrationRiskMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskTypeDomain_787cbd76-b285-4b50-b1a7-1c971d3f2728" xlink:to="loc_us-gaap_CustomerConcentrationRiskMember_5296a623-b6a3-472c-b302-fc34f40df0d2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskPercentage1_47423f2c-f7df-4b5b-b5d2-9481ea379e5d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskPercentage1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskLineItems_e54802ae-4817-4e0c-ac42-391ad451f62b" xlink:to="loc_us-gaap_ConcentrationRiskPercentage1_47423f2c-f7df-4b5b-b5d2-9481ea379e5d" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.kns.com/role/RESTRUCTURINGANDCANCELLATIONOFPROJECTNarrativeDetails" xlink:type="simple" xlink:href="klic-20240330.xsd#RESTRUCTURINGANDCANCELLATIONOFPROJECTNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.kns.com/role/RESTRUCTURINGANDCANCELLATIONOFPROJECTNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestructuringCostAndReserveLineItems_8a8ec2bb-9ede-4b24-b2ae-590618cfdd62" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RestructuringCostAndReserveLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRestructuringAndRelatedCostsTable_626115d9-dfe8-4da7-ab6f-68a98bdd2668" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfRestructuringAndRelatedCostsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RestructuringCostAndReserveLineItems_8a8ec2bb-9ede-4b24-b2ae-590618cfdd62" xlink:to="loc_us-gaap_ScheduleOfRestructuringAndRelatedCostsTable_626115d9-dfe8-4da7-ab6f-68a98bdd2668" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ImpairedLongLivedAssetsHeldAndUsedByTypeAxis_2782e59c-6921-41b2-a02f-4023709ee760" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ImpairedLongLivedAssetsHeldAndUsedByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRestructuringAndRelatedCostsTable_626115d9-dfe8-4da7-ab6f-68a98bdd2668" xlink:to="loc_us-gaap_ImpairedLongLivedAssetsHeldAndUsedByTypeAxis_2782e59c-6921-41b2-a02f-4023709ee760" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ImpairedLongLivedAssetsHeldAndUsedAssetNameDomain_2782e59c-6921-41b2-a02f-4023709ee760_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ImpairedLongLivedAssetsHeldAndUsedAssetNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ImpairedLongLivedAssetsHeldAndUsedByTypeAxis_2782e59c-6921-41b2-a02f-4023709ee760" xlink:to="loc_us-gaap_ImpairedLongLivedAssetsHeldAndUsedAssetNameDomain_2782e59c-6921-41b2-a02f-4023709ee760_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ImpairedLongLivedAssetsHeldAndUsedAssetNameDomain_70339fa0-8971-4734-a83a-e9c7581d6097" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ImpairedLongLivedAssetsHeldAndUsedAssetNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ImpairedLongLivedAssetsHeldAndUsedByTypeAxis_2782e59c-6921-41b2-a02f-4023709ee760" xlink:to="loc_us-gaap_ImpairedLongLivedAssetsHeldAndUsedAssetNameDomain_70339fa0-8971-4734-a83a-e9c7581d6097" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentMember_b87dee81-ad1a-4aa8-a549-c591f52f03eb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ImpairedLongLivedAssetsHeldAndUsedAssetNameDomain_70339fa0-8971-4734-a83a-e9c7581d6097" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentMember_b87dee81-ad1a-4aa8-a549-c591f52f03eb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_OperatingRightOfUseAssetsMember_0675c515-2ef9-4ebb-a019-17e879126fa2" xlink:href="klic-20240330.xsd#klic_OperatingRightOfUseAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ImpairedLongLivedAssetsHeldAndUsedAssetNameDomain_70339fa0-8971-4734-a83a-e9c7581d6097" xlink:to="loc_klic_OperatingRightOfUseAssetsMember_0675c515-2ef9-4ebb-a019-17e879126fa2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetRetirementObligationCostsMember_a5418672-c2e9-4184-a9fc-81e63a010f53" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AssetRetirementObligationCostsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ImpairedLongLivedAssetsHeldAndUsedAssetNameDomain_70339fa0-8971-4734-a83a-e9c7581d6097" xlink:to="loc_us-gaap_AssetRetirementObligationCostsMember_a5418672-c2e9-4184-a9fc-81e63a010f53" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryWriteDown_8ae08a7b-b0f9-46e4-962b-b107df33e6c8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_InventoryWriteDown"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestructuringCostAndReserveLineItems_8a8ec2bb-9ede-4b24-b2ae-590618cfdd62" xlink:to="loc_us-gaap_InventoryWriteDown_8ae08a7b-b0f9-46e4-962b-b107df33e6c8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeveranceCosts1_e78b6a4a-26e8-413f-95f7-58e7ffc57255" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SeveranceCosts1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestructuringCostAndReserveLineItems_8a8ec2bb-9ede-4b24-b2ae-590618cfdd62" xlink:to="loc_us-gaap_SeveranceCosts1_e78b6a4a-26e8-413f-95f7-58e7ffc57255" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_PurchaseOrderCancellationExpense_0f7476ac-a990-4586-a3a0-1172794c6739" xlink:href="klic-20240330.xsd#klic_PurchaseOrderCancellationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestructuringCostAndReserveLineItems_8a8ec2bb-9ede-4b24-b2ae-590618cfdd62" xlink:to="loc_klic_PurchaseOrderCancellationExpense_0f7476ac-a990-4586-a3a0-1172794c6739" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ImpairmentOfLongLivedAssetsHeldForUse_c99aa91c-c7eb-4f12-8842-1cc89fc9935d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ImpairmentOfLongLivedAssetsHeldForUse"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestructuringCostAndReserveLineItems_8a8ec2bb-9ede-4b24-b2ae-590618cfdd62" xlink:to="loc_us-gaap_ImpairmentOfLongLivedAssetsHeldForUse_c99aa91c-c7eb-4f12-8842-1cc89fc9935d" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://xbrl.sec.gov/ecd/AwardTypeOnly" xlink:type="simple" xlink:href="https://xbrl.sec.gov/ecd/2023/ecd-2023.xsd#AwardTypeOnly"/>
  <link:definitionLink xlink:role="http://xbrl.sec.gov/ecd/AwardTypeOnly" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_RelativeTSRPerformanceShareUnitsMember_873d6f90-6703-463c-9c88-706e4e8c92de" xlink:href="klic-20240330.xsd#klic_RelativeTSRPerformanceShareUnitsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" xlink:to="loc_klic_RelativeTSRPerformanceShareUnitsMember_873d6f90-6703-463c-9c88-706e4e8c92de" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_RevenueGrowthPerformanceShareUnitsMember_e64c9a3a-a16d-46a3-983d-8a16f64c571f" xlink:href="klic-20240330.xsd#klic_RevenueGrowthPerformanceShareUnitsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" xlink:to="loc_klic_RevenueGrowthPerformanceShareUnitsMember_e64c9a3a-a16d-46a3-983d-8a16f64c571f" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_TimeBasedRestrictedShareUnitsMember_da255ccb-31d4-46ed-a76e-5363b2beafba" xlink:href="klic-20240330.xsd#klic_TimeBasedRestrictedShareUnitsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" xlink:to="loc_klic_TimeBasedRestrictedShareUnitsMember_da255ccb-31d4-46ed-a76e-5363b2beafba" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_TimebasedRSUMember_7a73e39e-d338-40dd-9ca3-e27cb3e74012" xlink:href="klic-20240330.xsd#klic_TimebasedRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" xlink:to="loc_klic_TimebasedRSUMember_7a73e39e-d338-40dd-9ca3-e27cb3e74012" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_RelativeTSRPSUMember_0f817936-3117-46aa-8d25-1881220aa3e7" xlink:href="klic-20240330.xsd#klic_RelativeTSRPSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" xlink:to="loc_klic_RelativeTSRPSUMember_0f817936-3117-46aa-8d25-1881220aa3e7" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_klic_SpecialGrowthPSUMember_10f36afc-3c8c-4352-8031-dcad56246fe0" xlink:href="klic-20240330.xsd#klic_SpecialGrowthPSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" xlink:to="loc_klic_SpecialGrowthPSUMember_10f36afc-3c8c-4352-8031-dcad56246fe0" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" xlink:to="loc_us-gaap_CommonStockMember" xlink:type="arc" order="10"/>
  </link:definitionLink>
</link:linkbase>
