<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with Wdesk from Workiva-->
<!--Copyright 2022 Workiva-->
<!--r:86a1e2db-1400-41fb-85b4-4554b6581965,g:5b4df08d-9792-4857-9e76-48d287264d95-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://www.lakeland.com/role/CONSOLIDATEDSTATEMENTSOFCHANGESINSTOCKHOLDERSEQUITYunaudited" xlink:type="simple" xlink:href="lkfn-20220930.xsd#CONSOLIDATEDSTATEMENTSOFCHANGESINSTOCKHOLDERSEQUITYunaudited"/>
  <link:definitionLink xlink:role="http://www.lakeland.com/role/CONSOLIDATEDSTATEMENTSOFCHANGESINSTOCKHOLDERSEQUITYunaudited" xlink:type="extended" id="ied566c8428bf4996ad32485537741d7e_CONSOLIDATEDSTATEMENTSOFCHANGESINSTOCKHOLDERSEQUITYunaudited">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_806abe44-2120-4c55-9144-d3eb58ae32e1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_1950e19d-413f-4bb1-82fd-9db177f1bb82" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_806abe44-2120-4c55-9144-d3eb58ae32e1" xlink:to="loc_us-gaap_CommonStockSharesOutstanding_1950e19d-413f-4bb1-82fd-9db177f1bb82" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_e8773092-7d0d-4f1e-8629-8d1901bf7652" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_806abe44-2120-4c55-9144-d3eb58ae32e1" xlink:to="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_e8773092-7d0d-4f1e-8629-8d1901bf7652" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountingStandardsUpdateExtensibleList_066bd8e1-0e63-4ed8-8a93-aebdbd182bb4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccountingStandardsUpdateExtensibleList"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_806abe44-2120-4c55-9144-d3eb58ae32e1" xlink:to="loc_us-gaap_AccountingStandardsUpdateExtensibleList_066bd8e1-0e63-4ed8-8a93-aebdbd182bb4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_8097b2b5-3d7e-47d1-8d70-a53d90df7c6f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_806abe44-2120-4c55-9144-d3eb58ae32e1" xlink:to="loc_us-gaap_NetIncomeLoss_8097b2b5-3d7e-47d1-8d70-a53d90df7c6f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_4ad0fd73-87ae-4c0a-a8e8-a2e465ee1c68" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_806abe44-2120-4c55-9144-d3eb58ae32e1" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_4ad0fd73-87ae-4c0a-a8e8-a2e465ee1c68" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DividendsCommonStockCash_9377932f-2718-4270-b662-affb9d69d4bb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DividendsCommonStockCash"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_806abe44-2120-4c55-9144-d3eb58ae32e1" xlink:to="loc_us-gaap_DividendsCommonStockCash_9377932f-2718-4270-b662-affb9d69d4bb" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_TreasuryStockSharesPurchased_861afcf8-355d-4345-8065-56085c7c18b2" xlink:href="lkfn-20220930.xsd#lkfn_TreasuryStockSharesPurchased"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_806abe44-2120-4c55-9144-d3eb58ae32e1" xlink:to="loc_lkfn_TreasuryStockSharesPurchased_861afcf8-355d-4345-8065-56085c7c18b2" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_TreasurySharesPurchasedDeferredDirectorsPlan_5d41a590-578a-43f7-b685-1cf915c57f85" xlink:href="lkfn-20220930.xsd#lkfn_TreasurySharesPurchasedDeferredDirectorsPlan"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_806abe44-2120-4c55-9144-d3eb58ae32e1" xlink:to="loc_lkfn_TreasurySharesPurchasedDeferredDirectorsPlan_5d41a590-578a-43f7-b685-1cf915c57f85" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_TreasuryStockSoldAndDistributedUnderDeferredDirectorsPlanShares_c0e81750-f5bf-45b4-996a-5083195c0e67" xlink:href="lkfn-20220930.xsd#lkfn_TreasuryStockSoldAndDistributedUnderDeferredDirectorsPlanShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_806abe44-2120-4c55-9144-d3eb58ae32e1" xlink:to="loc_lkfn_TreasuryStockSoldAndDistributedUnderDeferredDirectorsPlanShares_c0e81750-f5bf-45b4-996a-5083195c0e67" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_TreasuryStockSoldAndDistributedUnderDeferredDirectorsPlanValue_feb0b78f-761c-4c77-97a2-bfaf31b27e90" xlink:href="lkfn-20220930.xsd#lkfn_TreasuryStockSoldAndDistributedUnderDeferredDirectorsPlanValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_806abe44-2120-4c55-9144-d3eb58ae32e1" xlink:to="loc_lkfn_TreasuryStockSoldAndDistributedUnderDeferredDirectorsPlanValue_feb0b78f-761c-4c77-97a2-bfaf31b27e90" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensation_15262652-fe05-4182-b02f-1a04229b40a2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_806abe44-2120-4c55-9144-d3eb58ae32e1" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensation_15262652-fe05-4182-b02f-1a04229b40a2" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueShareBasedCompensation_7d3f2eae-2250-4f92-b519-07a2b4ac7699" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockIssuedDuringPeriodValueShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_806abe44-2120-4c55-9144-d3eb58ae32e1" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueShareBasedCompensation_7d3f2eae-2250-4f92-b519-07a2b4ac7699" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_1cf0afa0-bc3c-4b7d-8180-28261194f11a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_806abe44-2120-4c55-9144-d3eb58ae32e1" xlink:to="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_1cf0afa0-bc3c-4b7d-8180-28261194f11a" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_da24be2e-19e3-4bf3-8939-1159661ebaf1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_b3eaac3b-a16f-4b88-b74d-a301b0cad62f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_544518a3-a5fe-4cff-ab2c-21234c977eca" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_806abe44-2120-4c55-9144-d3eb58ae32e1" xlink:to="loc_us-gaap_StatementTable_544518a3-a5fe-4cff-ab2c-21234c977eca" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_76b698bd-350f-479a-acc7-83b75594bdd0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_544518a3-a5fe-4cff-ab2c-21234c977eca" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_76b698bd-350f-479a-acc7-83b75594bdd0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_76b698bd-350f-479a-acc7-83b75594bdd0_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_76b698bd-350f-479a-acc7-83b75594bdd0" xlink:to="loc_us-gaap_EquityComponentDomain_76b698bd-350f-479a-acc7-83b75594bdd0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_58483bb7-7430-45c0-9ee7-c73fe2f8a923" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_76b698bd-350f-479a-acc7-83b75594bdd0" xlink:to="loc_us-gaap_EquityComponentDomain_58483bb7-7430-45c0-9ee7-c73fe2f8a923" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_13c5af15-781e-4a1f-a463-135341cdb567" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_58483bb7-7430-45c0-9ee7-c73fe2f8a923" xlink:to="loc_us-gaap_CommonStockMember_13c5af15-781e-4a1f-a463-135341cdb567" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsMember_76746845-2974-4434-9438-5357d7116ad6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RetainedEarningsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_58483bb7-7430-45c0-9ee7-c73fe2f8a923" xlink:to="loc_us-gaap_RetainedEarningsMember_76746845-2974-4434-9438-5357d7116ad6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_61bf006c-f15e-45c9-91a7-cd96b5001c9c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_58483bb7-7430-45c0-9ee7-c73fe2f8a923" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_61bf006c-f15e-45c9-91a7-cd96b5001c9c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockCommonMember_8b932292-f6b9-4ff8-8068-a52c9b453873" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TreasuryStockCommonMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_58483bb7-7430-45c0-9ee7-c73fe2f8a923" xlink:to="loc_us-gaap_TreasuryStockCommonMember_8b932292-f6b9-4ff8-8068-a52c9b453873" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ParentMember_ef2e4661-86bf-485f-a5ff-a4b34629554f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ParentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_58483bb7-7430-45c0-9ee7-c73fe2f8a923" xlink:to="loc_us-gaap_ParentMember_ef2e4661-86bf-485f-a5ff-a4b34629554f" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NoncontrollingInterestMember_bf4b79ed-e2a5-4f39-beca-e1739665280c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NoncontrollingInterestMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_58483bb7-7430-45c0-9ee7-c73fe2f8a923" xlink:to="loc_us-gaap_NoncontrollingInterestMember_bf4b79ed-e2a5-4f39-beca-e1739665280c" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CumulativeEffectPeriodOfAdoptionAxis_a7c1fd6a-6506-4ea6-b606-d81cab67851d" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_CumulativeEffectPeriodOfAdoptionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_544518a3-a5fe-4cff-ab2c-21234c977eca" xlink:to="loc_srt_CumulativeEffectPeriodOfAdoptionAxis_a7c1fd6a-6506-4ea6-b606-d81cab67851d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_a7c1fd6a-6506-4ea6-b606-d81cab67851d_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_CumulativeEffectPeriodOfAdoptionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CumulativeEffectPeriodOfAdoptionAxis_a7c1fd6a-6506-4ea6-b606-d81cab67851d" xlink:to="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_a7c1fd6a-6506-4ea6-b606-d81cab67851d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_3c0be4e3-67d8-4a42-a157-f1a13fdc17db" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_CumulativeEffectPeriodOfAdoptionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CumulativeEffectPeriodOfAdoptionAxis_a7c1fd6a-6506-4ea6-b606-d81cab67851d" xlink:to="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_3c0be4e3-67d8-4a42-a157-f1a13fdc17db" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CumulativeEffectPeriodOfAdoptionAdjustmentMember_34d5d30f-f017-4cd0-895c-a01080113f49" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_CumulativeEffectPeriodOfAdoptionAdjustmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_3c0be4e3-67d8-4a42-a157-f1a13fdc17db" xlink:to="loc_srt_CumulativeEffectPeriodOfAdoptionAdjustmentMember_34d5d30f-f017-4cd0-895c-a01080113f49" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.lakeland.com/role/SECURITIESSummaryofAvailableForSaleandHeldtoMaturitySecuritiesDetails" xlink:type="simple" xlink:href="lkfn-20220930.xsd#SECURITIESSummaryofAvailableForSaleandHeldtoMaturitySecuritiesDetails"/>
  <link:definitionLink xlink:role="http://www.lakeland.com/role/SECURITIESSummaryofAvailableForSaleandHeldtoMaturitySecuritiesDetails" xlink:type="extended" id="i50e7ecd3884d486aa5ffc31d2bc4c8bd_SECURITIESSummaryofAvailableForSaleandHeldtoMaturitySecuritiesDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_f2afb014-4b35-4bfc-9030-0fc66c271b11" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesAbstract_821d2c1d-7865-46a1-811f-ae5eca90d7ca" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AvailableForSaleSecuritiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_f2afb014-4b35-4bfc-9030-0fc66c271b11" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesAbstract_821d2c1d-7865-46a1-811f-ae5eca90d7ca" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleAmortizedCostExcludingAccruedInterestBeforeAllowanceForCreditLoss_e3999444-ea44-4a56-b090-a63f2bebd19b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtSecuritiesAvailableForSaleAmortizedCostExcludingAccruedInterestBeforeAllowanceForCreditLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesAbstract_821d2c1d-7865-46a1-811f-ae5eca90d7ca" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleAmortizedCostExcludingAccruedInterestBeforeAllowanceForCreditLoss_e3999444-ea44-4a56-b090-a63f2bebd19b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax_8e3242d4-4033-4560-843b-1a651c216987" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesAbstract_821d2c1d-7865-46a1-811f-ae5eca90d7ca" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax_8e3242d4-4033-4560-843b-1a651c216987" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax_9e62ae6d-eac5-4bb8-84be-ee3ccd733a18" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesAbstract_821d2c1d-7865-46a1-811f-ae5eca90d7ca" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax_9e62ae6d-eac5-4bb8-84be-ee3ccd733a18" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleAmortizedCostAllowanceForCreditLossExcludingAccruedInterest_06507399-82a9-48af-8141-2e81817bd5fd" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtSecuritiesAvailableForSaleAmortizedCostAllowanceForCreditLossExcludingAccruedInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesAbstract_821d2c1d-7865-46a1-811f-ae5eca90d7ca" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleAmortizedCostAllowanceForCreditLossExcludingAccruedInterest_06507399-82a9-48af-8141-2e81817bd5fd" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleExcludingAccruedInterest_9a41dba7-f085-4fe9-885c-f70b53167ba0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtSecuritiesAvailableForSaleExcludingAccruedInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesAbstract_821d2c1d-7865-46a1-811f-ae5eca90d7ca" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleExcludingAccruedInterest_9a41dba7-f085-4fe9-885c-f70b53167ba0" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_8fefb7a3-cbd0-44c4-b37d-e90df0fd9520" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtSecuritiesAvailableForSaleTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_f2afb014-4b35-4bfc-9030-0fc66c271b11" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_8fefb7a3-cbd0-44c4-b37d-e90df0fd9520" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_ea1d9d3b-01a3-4560-b963-875415613acb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_8fefb7a3-cbd0-44c4-b37d-e90df0fd9520" xlink:to="loc_us-gaap_FinancialInstrumentAxis_ea1d9d3b-01a3-4560-b963-875415613acb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_ea1d9d3b-01a3-4560-b963-875415613acb_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_ea1d9d3b-01a3-4560-b963-875415613acb" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_ea1d9d3b-01a3-4560-b963-875415613acb_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_77355d63-50b2-4ce9-8fd1-a560c0f1c939" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_ea1d9d3b-01a3-4560-b963-875415613acb" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_77355d63-50b2-4ce9-8fd1-a560c0f1c939" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USTreasurySecuritiesMember_c0ac1032-6d49-4dfb-b21c-076e3a9d0890" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_USTreasurySecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_77355d63-50b2-4ce9-8fd1-a560c0f1c939" xlink:to="loc_us-gaap_USTreasurySecuritiesMember_c0ac1032-6d49-4dfb-b21c-076e3a9d0890" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USGovernmentSponsoredEnterprisesDebtSecuritiesMember_444c8b61-ba8e-4842-98dc-4b1365ef9382" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_USGovernmentSponsoredEnterprisesDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_77355d63-50b2-4ce9-8fd1-a560c0f1c939" xlink:to="loc_us-gaap_USGovernmentSponsoredEnterprisesDebtSecuritiesMember_444c8b61-ba8e-4842-98dc-4b1365ef9382" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResidentialMortgageBackedSecuritiesMember_61f98e68-ee32-4118-86ba-f7bd8961b588" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ResidentialMortgageBackedSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_77355d63-50b2-4ce9-8fd1-a560c0f1c939" xlink:to="loc_us-gaap_ResidentialMortgageBackedSecuritiesMember_61f98e68-ee32-4118-86ba-f7bd8961b588" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialMortgageBackedSecuritiesMember_282b61d6-42d9-4fd2-83fe-645b842663b4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommercialMortgageBackedSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_77355d63-50b2-4ce9-8fd1-a560c0f1c939" xlink:to="loc_us-gaap_CommercialMortgageBackedSecuritiesMember_282b61d6-42d9-4fd2-83fe-645b842663b4" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USStatesAndPoliticalSubdivisionsMember_57cc349f-2434-4627-a6a4-fcd04778bdc6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_USStatesAndPoliticalSubdivisionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_77355d63-50b2-4ce9-8fd1-a560c0f1c939" xlink:to="loc_us-gaap_USStatesAndPoliticalSubdivisionsMember_57cc349f-2434-4627-a6a4-fcd04778bdc6" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_cca669fe-8b2d-4697-87e7-c51026be850e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesAbstract_ac16f326-2aef-4072-9f1f-5853d30d8322" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_HeldToMaturitySecuritiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_cca669fe-8b2d-4697-87e7-c51026be850e" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesAbstract_ac16f326-2aef-4072-9f1f-5853d30d8322" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecurities_f3473c55-fc6b-48e2-9bd3-e4981566a767" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_HeldToMaturitySecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HeldToMaturitySecuritiesAbstract_ac16f326-2aef-4072-9f1f-5853d30d8322" xlink:to="loc_us-gaap_HeldToMaturitySecurities_f3473c55-fc6b-48e2-9bd3-e4981566a767" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingGain_a7997d5b-7a35-4035-998e-96bac9f6f9c9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingGain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HeldToMaturitySecuritiesAbstract_ac16f326-2aef-4072-9f1f-5853d30d8322" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingGain_a7997d5b-7a35-4035-998e-96bac9f6f9c9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingLoss_5ac6626a-f3ad-4a0c-b9a8-d80e19894767" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HeldToMaturitySecuritiesAbstract_ac16f326-2aef-4072-9f1f-5853d30d8322" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingLoss_5ac6626a-f3ad-4a0c-b9a8-d80e19894767" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesHeldToMaturityAllowanceForCreditLoss_df24771d-e26a-4e19-8790-e9c80929ed6b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtSecuritiesHeldToMaturityAllowanceForCreditLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HeldToMaturitySecuritiesAbstract_ac16f326-2aef-4072-9f1f-5853d30d8322" xlink:to="loc_us-gaap_DebtSecuritiesHeldToMaturityAllowanceForCreditLoss_df24771d-e26a-4e19-8790-e9c80929ed6b" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesFairValue_620ec1eb-0b72-43ec-8026-32cb7ff5155b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_HeldToMaturitySecuritiesFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HeldToMaturitySecuritiesAbstract_ac16f326-2aef-4072-9f1f-5853d30d8322" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesFairValue_620ec1eb-0b72-43ec-8026-32cb7ff5155b" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesHeldToMaturityAllowanceForCreditLossExcludingAccruedInterest_f879c28a-94a5-48eb-99a0-091c65d1a568" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtSecuritiesHeldToMaturityAllowanceForCreditLossExcludingAccruedInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_cca669fe-8b2d-4697-87e7-c51026be850e" xlink:to="loc_us-gaap_DebtSecuritiesHeldToMaturityAllowanceForCreditLossExcludingAccruedInterest_f879c28a-94a5-48eb-99a0-091c65d1a568" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesTable_404bb075-9768-4f65-8f40-62ffe39eeace" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfHeldToMaturitySecuritiesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_cca669fe-8b2d-4697-87e7-c51026be850e" xlink:to="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesTable_404bb075-9768-4f65-8f40-62ffe39eeace" xlink:type="arc" order="2" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_dd411d13-25e9-4360-b2b7-5f6c0b77cfe1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesTable_404bb075-9768-4f65-8f40-62ffe39eeace" xlink:to="loc_us-gaap_FinancialInstrumentAxis_dd411d13-25e9-4360-b2b7-5f6c0b77cfe1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_dd411d13-25e9-4360-b2b7-5f6c0b77cfe1_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_9f261660-9879-4d93-91fa-de62e3397474" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USStatesAndPoliticalSubdivisionsMember_4b751505-609d-4441-b377-cbabbb6f789d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_USStatesAndPoliticalSubdivisionsMember"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.lakeland.com/role/SECURITIESUnrealizedLossAnalysisonAvailableforSaleandHeldtoMaturitySecuritiesDetails" xlink:type="simple" xlink:href="lkfn-20220930.xsd#SECURITIESUnrealizedLossAnalysisonAvailableforSaleandHeldtoMaturitySecuritiesDetails"/>
  <link:definitionLink xlink:role="http://www.lakeland.com/role/SECURITIESUnrealizedLossAnalysisonAvailableforSaleandHeldtoMaturitySecuritiesDetails" xlink:type="extended" id="i3b1c7ab4bdb6443ba8c06f8b719a1b51_SECURITIESUnrealizedLossAnalysisonAvailableforSaleandHeldtoMaturitySecuritiesDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_2cb4a05a-2d3a-4fdc-b4e8-edb511b0b89b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12Months_df4dd3fe-acf7-4d08-8b0b-35df19e641ed" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12Months"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_2cb4a05a-2d3a-4fdc-b4e8-edb511b0b89b" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12Months_df4dd3fe-acf7-4d08-8b0b-35df19e641ed" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLonger_cef4213e-dcb6-44c6-bc0a-ddfe598dac68" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLonger"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_2cb4a05a-2d3a-4fdc-b4e8-edb511b0b89b" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLonger_cef4213e-dcb6-44c6-bc0a-ddfe598dac68" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPosition_0d8a6c87-d857-4d1e-80dd-f7aa65301a97" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPosition"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_2cb4a05a-2d3a-4fdc-b4e8-edb511b0b89b" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPosition_0d8a6c87-d857-4d1e-80dd-f7aa65301a97" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12MonthsAccumulatedLoss_4818e46a-2f5a-4785-a60b-b0340d92d8b2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12MonthsAccumulatedLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_2cb4a05a-2d3a-4fdc-b4e8-edb511b0b89b" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12MonthsAccumulatedLoss_4818e46a-2f5a-4785-a60b-b0340d92d8b2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLongerAccumulatedLoss_17fde8c7-62fe-465e-aeb8-45eff8e17be2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLongerAccumulatedLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_2cb4a05a-2d3a-4fdc-b4e8-edb511b0b89b" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLongerAccumulatedLoss_17fde8c7-62fe-465e-aeb8-45eff8e17be2" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPositionAccumulatedLoss_1a546ec2-e22c-4840-a62d-53d7f69178da" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPositionAccumulatedLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_2cb4a05a-2d3a-4fdc-b4e8-edb511b0b89b" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPositionAccumulatedLoss_1a546ec2-e22c-4840-a62d-53d7f69178da" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_088c67b0-8453-4b1d-a2e3-7065229c1652" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtSecuritiesAvailableForSaleTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_2cb4a05a-2d3a-4fdc-b4e8-edb511b0b89b" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_088c67b0-8453-4b1d-a2e3-7065229c1652" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_b118e0c2-2aa2-4503-932f-f8472070e53a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_088c67b0-8453-4b1d-a2e3-7065229c1652" xlink:to="loc_us-gaap_FinancialInstrumentAxis_b118e0c2-2aa2-4503-932f-f8472070e53a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_b118e0c2-2aa2-4503-932f-f8472070e53a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_b118e0c2-2aa2-4503-932f-f8472070e53a" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_b118e0c2-2aa2-4503-932f-f8472070e53a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_277e0a33-a3ae-480d-97e0-3e85f9332993" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_b118e0c2-2aa2-4503-932f-f8472070e53a" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_277e0a33-a3ae-480d-97e0-3e85f9332993" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USTreasurySecuritiesMember_6de0db75-8af8-4aea-a9ba-fda880d82fb1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_USTreasurySecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_277e0a33-a3ae-480d-97e0-3e85f9332993" xlink:to="loc_us-gaap_USTreasurySecuritiesMember_6de0db75-8af8-4aea-a9ba-fda880d82fb1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USGovernmentSponsoredEnterprisesDebtSecuritiesMember_cd4382bd-64a4-492d-a078-9cfbb20e7c29" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_USGovernmentSponsoredEnterprisesDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_277e0a33-a3ae-480d-97e0-3e85f9332993" xlink:to="loc_us-gaap_USGovernmentSponsoredEnterprisesDebtSecuritiesMember_cd4382bd-64a4-492d-a078-9cfbb20e7c29" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResidentialMortgageBackedSecuritiesMember_b82eee1a-c2c6-4544-ab79-584aae9c3a36" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ResidentialMortgageBackedSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_277e0a33-a3ae-480d-97e0-3e85f9332993" xlink:to="loc_us-gaap_ResidentialMortgageBackedSecuritiesMember_b82eee1a-c2c6-4544-ab79-584aae9c3a36" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USStatesAndPoliticalSubdivisionsMember_fcd2c1b6-c154-45e9-9ae3-58b91317c28b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_USStatesAndPoliticalSubdivisionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_277e0a33-a3ae-480d-97e0-3e85f9332993" xlink:to="loc_us-gaap_USStatesAndPoliticalSubdivisionsMember_fcd2c1b6-c154-45e9-9ae3-58b91317c28b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_eeb74ea6-94b3-408a-a8f7-e45b12293b90" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionLessThanTwelveMonthsFairValue_50af55e4-f6a9-4d53-904c-d92733125cd9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionLessThanTwelveMonthsFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_eeb74ea6-94b3-408a-a8f7-e45b12293b90" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionLessThanTwelveMonthsFairValue_50af55e4-f6a9-4d53-904c-d92733125cd9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionTwelveMonthsOrLongerFairValue_01dc42db-9ae4-469b-af14-cdb5de03cb14" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionTwelveMonthsOrLongerFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_eeb74ea6-94b3-408a-a8f7-e45b12293b90" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionTwelveMonthsOrLongerFairValue_01dc42db-9ae4-469b-af14-cdb5de03cb14" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionFairValue_9a62ae56-84dc-4af2-bd31-cba56e646887" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_eeb74ea6-94b3-408a-a8f7-e45b12293b90" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionFairValue_9a62ae56-84dc-4af2-bd31-cba56e646887" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionLessThan12MonthsAccumulatedLoss_7669dc6b-4f7f-4c23-a2c4-1f44caed0551" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionLessThan12MonthsAccumulatedLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_eeb74ea6-94b3-408a-a8f7-e45b12293b90" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionLessThan12MonthsAccumulatedLoss_7669dc6b-4f7f-4c23-a2c4-1f44caed0551" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPosition12MonthsOrLongerAccumulatedLoss_9d7402b2-67c4-4b1a-95e9-78cd3b4fb5d7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPosition12MonthsOrLongerAccumulatedLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_eeb74ea6-94b3-408a-a8f7-e45b12293b90" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPosition12MonthsOrLongerAccumulatedLoss_9d7402b2-67c4-4b1a-95e9-78cd3b4fb5d7" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionAccumulatedLoss_75dd7e45-f6f7-4968-a2a0-272837801a9d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionAccumulatedLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_eeb74ea6-94b3-408a-a8f7-e45b12293b90" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionAccumulatedLoss_75dd7e45-f6f7-4968-a2a0-272837801a9d" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesTable_ba199951-e55b-42d3-bea9-795015c66353" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfHeldToMaturitySecuritiesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_eeb74ea6-94b3-408a-a8f7-e45b12293b90" xlink:to="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesTable_ba199951-e55b-42d3-bea9-795015c66353" xlink:type="arc" order="2" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_5d89d920-3c65-4d1f-b82b-06af768f0b60" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesTable_ba199951-e55b-42d3-bea9-795015c66353" xlink:to="loc_us-gaap_FinancialInstrumentAxis_5d89d920-3c65-4d1f-b82b-06af768f0b60" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_5d89d920-3c65-4d1f-b82b-06af768f0b60_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_edbb732b-d933-4090-8bc0-d7308fcb78cb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USStatesAndPoliticalSubdivisionsMember_b5e418ea-4a0c-4284-97ae-96b4e2e4afc1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_USStatesAndPoliticalSubdivisionsMember"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.lakeland.com/role/SECURITIESQuantitativeDisclosureofAvailableForSaleofSecuritiesDetails" xlink:type="simple" xlink:href="lkfn-20220930.xsd#SECURITIESQuantitativeDisclosureofAvailableForSaleofSecuritiesDetails"/>
  <link:definitionLink xlink:role="http://www.lakeland.com/role/SECURITIESQuantitativeDisclosureofAvailableForSaleofSecuritiesDetails" xlink:type="extended" id="i144e4f0a4a8d4eddbf11f6c8cf5d0fa0_SECURITIESQuantitativeDisclosureofAvailableForSaleofSecuritiesDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_b204759b-2638-4759-82bb-231bc58a487e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_NumberOfSecuritiesWithUnrealizedLossesAbstract_32217c0a-0426-403e-820a-e0bd370fbe0a" xlink:href="lkfn-20220930.xsd#lkfn_NumberOfSecuritiesWithUnrealizedLossesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_b204759b-2638-4759-82bb-231bc58a487e" xlink:to="loc_lkfn_NumberOfSecuritiesWithUnrealizedLossesAbstract_32217c0a-0426-403e-820a-e0bd370fbe0a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_NumberOfAvailableForSaleSecuritiesInUnrealizedLossPositionsQualitativeDisclosureNumberOfPositionsLessThan12Months_844249e9-6748-46c0-8a97-3ffe60fcdee1" xlink:href="lkfn-20220930.xsd#lkfn_NumberOfAvailableForSaleSecuritiesInUnrealizedLossPositionsQualitativeDisclosureNumberOfPositionsLessThan12Months"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_NumberOfSecuritiesWithUnrealizedLossesAbstract_32217c0a-0426-403e-820a-e0bd370fbe0a" xlink:to="loc_lkfn_NumberOfAvailableForSaleSecuritiesInUnrealizedLossPositionsQualitativeDisclosureNumberOfPositionsLessThan12Months_844249e9-6748-46c0-8a97-3ffe60fcdee1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_NumberOfAvailableForSaleSecuritiesInUnrealizedLossPositionsQualitativeDisclosureNumberOfPositions12MonthsOrMore_575b752d-da39-4386-b50c-488ca418e869" xlink:href="lkfn-20220930.xsd#lkfn_NumberOfAvailableForSaleSecuritiesInUnrealizedLossPositionsQualitativeDisclosureNumberOfPositions12MonthsOrMore"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_NumberOfSecuritiesWithUnrealizedLossesAbstract_32217c0a-0426-403e-820a-e0bd370fbe0a" xlink:to="loc_lkfn_NumberOfAvailableForSaleSecuritiesInUnrealizedLossPositionsQualitativeDisclosureNumberOfPositions12MonthsOrMore_575b752d-da39-4386-b50c-488ca418e869" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPositionNumberOfPositions_ad34e126-25c5-4f9b-a281-b92e26061d59" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPositionNumberOfPositions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_NumberOfSecuritiesWithUnrealizedLossesAbstract_32217c0a-0426-403e-820a-e0bd370fbe0a" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPositionNumberOfPositions_ad34e126-25c5-4f9b-a281-b92e26061d59" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_903febd7-a91f-4b78-b6cd-3d56cb5a0188" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtSecuritiesAvailableForSaleTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_b204759b-2638-4759-82bb-231bc58a487e" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_903febd7-a91f-4b78-b6cd-3d56cb5a0188" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_cf71bec8-77c6-4fea-99e5-e10975a52bcc" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_903febd7-a91f-4b78-b6cd-3d56cb5a0188" xlink:to="loc_us-gaap_FinancialInstrumentAxis_cf71bec8-77c6-4fea-99e5-e10975a52bcc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_cf71bec8-77c6-4fea-99e5-e10975a52bcc_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_cf71bec8-77c6-4fea-99e5-e10975a52bcc" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_cf71bec8-77c6-4fea-99e5-e10975a52bcc_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_d38b2a16-7a99-4a39-88ef-a487831883e7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_cf71bec8-77c6-4fea-99e5-e10975a52bcc" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_d38b2a16-7a99-4a39-88ef-a487831883e7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USTreasurySecuritiesMember_8ba98044-de58-4ee2-b09e-84b0617b745a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_USTreasurySecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_d38b2a16-7a99-4a39-88ef-a487831883e7" xlink:to="loc_us-gaap_USTreasurySecuritiesMember_8ba98044-de58-4ee2-b09e-84b0617b745a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USGovernmentSponsoredEnterprisesDebtSecuritiesMember_08c9b22f-a14b-4382-a662-b0324091755e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_USGovernmentSponsoredEnterprisesDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_d38b2a16-7a99-4a39-88ef-a487831883e7" xlink:to="loc_us-gaap_USGovernmentSponsoredEnterprisesDebtSecuritiesMember_08c9b22f-a14b-4382-a662-b0324091755e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResidentialMortgageBackedSecuritiesMember_a04cd6d7-a969-415d-b66d-8d1986a1c06e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ResidentialMortgageBackedSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_d38b2a16-7a99-4a39-88ef-a487831883e7" xlink:to="loc_us-gaap_ResidentialMortgageBackedSecuritiesMember_a04cd6d7-a969-415d-b66d-8d1986a1c06e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialMortgageBackedSecuritiesMember_0e625044-3d46-4c44-94af-59aa4e5b5caa" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommercialMortgageBackedSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_d38b2a16-7a99-4a39-88ef-a487831883e7" xlink:to="loc_us-gaap_CommercialMortgageBackedSecuritiesMember_0e625044-3d46-4c44-94af-59aa4e5b5caa" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USStatesAndPoliticalSubdivisionsMember_face4cab-a29e-4a46-8c34-c6c9529dcb60" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_USStatesAndPoliticalSubdivisionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_d38b2a16-7a99-4a39-88ef-a487831883e7" xlink:to="loc_us-gaap_USStatesAndPoliticalSubdivisionsMember_face4cab-a29e-4a46-8c34-c6c9529dcb60" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.lakeland.com/role/SECURITIESQuantitativeDisclosureofHeldtoMaturitySecuritiesDetails" xlink:type="simple" xlink:href="lkfn-20220930.xsd#SECURITIESQuantitativeDisclosureofHeldtoMaturitySecuritiesDetails"/>
  <link:definitionLink xlink:role="http://www.lakeland.com/role/SECURITIESQuantitativeDisclosureofHeldtoMaturitySecuritiesDetails" xlink:type="extended" id="i20936e439c394731a4b0cf11aae21a3d_SECURITIESQuantitativeDisclosureofHeldtoMaturitySecuritiesDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_12e96350-77ce-44f6-881d-75f7f4f1b2ed" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_NumberOfHeldToMaturitySecuritiesInUnrealizedLossPositionsQualitativeDisclosureNumberOfPositionsLessThan12Months_8e12d224-3779-4720-bdbb-ef31401182d8" xlink:href="lkfn-20220930.xsd#lkfn_NumberOfHeldToMaturitySecuritiesInUnrealizedLossPositionsQualitativeDisclosureNumberOfPositionsLessThan12Months"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_12e96350-77ce-44f6-881d-75f7f4f1b2ed" xlink:to="loc_lkfn_NumberOfHeldToMaturitySecuritiesInUnrealizedLossPositionsQualitativeDisclosureNumberOfPositionsLessThan12Months_8e12d224-3779-4720-bdbb-ef31401182d8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_NumberOfHeldToMaturitySecuritiesInUnrealizedLossPositionsQualitativeDisclosureNumberOfPositions12MonthsOrMore_1820cedd-b294-4ee2-9473-a7709c5c7dc7" xlink:href="lkfn-20220930.xsd#lkfn_NumberOfHeldToMaturitySecuritiesInUnrealizedLossPositionsQualitativeDisclosureNumberOfPositions12MonthsOrMore"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_12e96350-77ce-44f6-881d-75f7f4f1b2ed" xlink:to="loc_lkfn_NumberOfHeldToMaturitySecuritiesInUnrealizedLossPositionsQualitativeDisclosureNumberOfPositions12MonthsOrMore_1820cedd-b294-4ee2-9473-a7709c5c7dc7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesInUnrealizedLossPositionsQualitativeDisclosureNumberOfPositions_2cc6f69b-f85e-490c-bcd9-f6abacc262b4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_HeldToMaturitySecuritiesInUnrealizedLossPositionsQualitativeDisclosureNumberOfPositions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_12e96350-77ce-44f6-881d-75f7f4f1b2ed" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesInUnrealizedLossPositionsQualitativeDisclosureNumberOfPositions_2cc6f69b-f85e-490c-bcd9-f6abacc262b4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesTable_c044adc6-8ab8-4972-b379-aab8a4b5a0e3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfHeldToMaturitySecuritiesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_12e96350-77ce-44f6-881d-75f7f4f1b2ed" xlink:to="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesTable_c044adc6-8ab8-4972-b379-aab8a4b5a0e3" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_ae1d24d5-bec9-468a-a09c-ffd19c6e0161" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesTable_c044adc6-8ab8-4972-b379-aab8a4b5a0e3" xlink:to="loc_us-gaap_FinancialInstrumentAxis_ae1d24d5-bec9-468a-a09c-ffd19c6e0161" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_ae1d24d5-bec9-468a-a09c-ffd19c6e0161_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_ae1d24d5-bec9-468a-a09c-ffd19c6e0161" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_ae1d24d5-bec9-468a-a09c-ffd19c6e0161_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_4b890a46-f3bb-4329-814a-c09c11f3085c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_ae1d24d5-bec9-468a-a09c-ffd19c6e0161" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_4b890a46-f3bb-4329-814a-c09c11f3085c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USTreasurySecuritiesMember_712afb41-16c9-48b4-91e4-8d4cec60eb08" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_USTreasurySecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_4b890a46-f3bb-4329-814a-c09c11f3085c" xlink:to="loc_us-gaap_USTreasurySecuritiesMember_712afb41-16c9-48b4-91e4-8d4cec60eb08" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USGovernmentSponsoredEnterprisesDebtSecuritiesMember_b353cd74-429d-4e6d-815e-38c0466bce73" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_USGovernmentSponsoredEnterprisesDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_4b890a46-f3bb-4329-814a-c09c11f3085c" xlink:to="loc_us-gaap_USGovernmentSponsoredEnterprisesDebtSecuritiesMember_b353cd74-429d-4e6d-815e-38c0466bce73" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResidentialMortgageBackedSecuritiesMember_101c5418-7341-4bc2-82e9-e0ed05775ee0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ResidentialMortgageBackedSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_4b890a46-f3bb-4329-814a-c09c11f3085c" xlink:to="loc_us-gaap_ResidentialMortgageBackedSecuritiesMember_101c5418-7341-4bc2-82e9-e0ed05775ee0" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialMortgageBackedSecuritiesMember_6825f032-ca55-4c22-bc2c-165581825408" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommercialMortgageBackedSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_4b890a46-f3bb-4329-814a-c09c11f3085c" xlink:to="loc_us-gaap_CommercialMortgageBackedSecuritiesMember_6825f032-ca55-4c22-bc2c-165581825408" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USStatesAndPoliticalSubdivisionsMember_e745eca7-6311-42db-99cb-6452d68481a3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_USStatesAndPoliticalSubdivisionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_4b890a46-f3bb-4329-814a-c09c11f3085c" xlink:to="loc_us-gaap_USStatesAndPoliticalSubdivisionsMember_e745eca7-6311-42db-99cb-6452d68481a3" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.lakeland.com/role/LOANSTotalLoansOutstandingDetails" xlink:type="simple" xlink:href="lkfn-20220930.xsd#LOANSTotalLoansOutstandingDetails"/>
  <link:definitionLink xlink:role="http://www.lakeland.com/role/LOANSTotalLoansOutstandingDetails" xlink:type="extended" id="i4c75fce54c304fa988375fb7470e8b6f_LOANSTotalLoansOutstandingDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_87b61860-ffac-4617-a0ca-3d049c98431a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccountsNotesAndLoansReceivableLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestBeforeAllowanceForCreditLossFeeAndLoanInProcess_5b87228a-5a39-452c-a4ac-776071c28f1e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestBeforeAllowanceForCreditLossFeeAndLoanInProcess"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_87b61860-ffac-4617-a0ca-3d049c98431a" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestBeforeAllowanceForCreditLossFeeAndLoanInProcess_5b87228a-5a39-452c-a4ac-776071c28f1e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_PercentageOfReceivablesBeforeCreditAllowanceCarryingAmount_72d01bf2-4926-48da-a996-c35877499138" xlink:href="lkfn-20220930.xsd#lkfn_PercentageOfReceivablesBeforeCreditAllowanceCarryingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_87b61860-ffac-4617-a0ca-3d049c98431a" xlink:to="loc_lkfn_PercentageOfReceivablesBeforeCreditAllowanceCarryingAmount_72d01bf2-4926-48da-a996-c35877499138" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableAllowanceForCreditLossExcludingAccruedInterest_3c1f927a-fe32-4bd8-8337-e713de2a9025" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableAllowanceForCreditLossExcludingAccruedInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_87b61860-ffac-4617-a0ca-3d049c98431a" xlink:to="loc_us-gaap_FinancingReceivableAllowanceForCreditLossExcludingAccruedInterest_3c1f927a-fe32-4bd8-8337-e713de2a9025" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableDeferredIncome_2a68b935-986d-4150-9e06-b3ef6ec583b2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableDeferredIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_87b61860-ffac-4617-a0ca-3d049c98431a" xlink:to="loc_us-gaap_FinancingReceivableDeferredIncome_2a68b935-986d-4150-9e06-b3ef6ec583b2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAfterAllowanceForCreditLoss_03211eb3-3fb9-409f-b7f1-529ce3c86b3d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestAfterAllowanceForCreditLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_87b61860-ffac-4617-a0ca-3d049c98431a" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAfterAllowanceForCreditLoss_03211eb3-3fb9-409f-b7f1-529ce3c86b3d" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_75804a36-2b80-4aca-abb5-59e349dcd6ae" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AccountsNotesAndLoansReceivableLineItems_87b61860-ffac-4617-a0ca-3d049c98431a" xlink:to="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_75804a36-2b80-4aca-abb5-59e349dcd6ae" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_6f085b94-3c70-40d2-88c6-808df38e2d6f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_75804a36-2b80-4aca-abb5-59e349dcd6ae" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_6f085b94-3c70-40d2-88c6-808df38e2d6f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_6f085b94-3c70-40d2-88c6-808df38e2d6f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_6f085b94-3c70-40d2-88c6-808df38e2d6f" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_6f085b94-3c70-40d2-88c6-808df38e2d6f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_7285ad7c-bccf-46c4-801f-2e2c4acd0a77" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_6f085b94-3c70-40d2-88c6-808df38e2d6f" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_7285ad7c-bccf-46c4-801f-2e2c4acd0a77" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_TotalCommercialAndIndustrialLoansMember_9ff2c6e0-fd61-4b0f-ae0f-d8071a4c3ef4" xlink:href="lkfn-20220930.xsd#lkfn_TotalCommercialAndIndustrialLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_7285ad7c-bccf-46c4-801f-2e2c4acd0a77" xlink:to="loc_lkfn_TotalCommercialAndIndustrialLoansMember_9ff2c6e0-fd61-4b0f-ae0f-d8071a4c3ef4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RealEstateLoanMember_49268361-8878-4d25-864a-b0d841177f81" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RealEstateLoanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_7285ad7c-bccf-46c4-801f-2e2c4acd0a77" xlink:to="loc_us-gaap_RealEstateLoanMember_49268361-8878-4d25-864a-b0d841177f81" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_AgriBusinessAndAgriculturalLoansMember_6fb07d6e-ab5e-4fca-a571-9c8f2c37df42" xlink:href="lkfn-20220930.xsd#lkfn_AgriBusinessAndAgriculturalLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_7285ad7c-bccf-46c4-801f-2e2c4acd0a77" xlink:to="loc_lkfn_AgriBusinessAndAgriculturalLoansMember_6fb07d6e-ab5e-4fca-a571-9c8f2c37df42" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_OtherCommercialLoansMember_c38945b9-249c-46b3-b326-6c3b70e4f1c4" xlink:href="lkfn-20220930.xsd#lkfn_OtherCommercialLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_7285ad7c-bccf-46c4-801f-2e2c4acd0a77" xlink:to="loc_lkfn_OtherCommercialLoansMember_c38945b9-249c-46b3-b326-6c3b70e4f1c4" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_TotalConsumer1To4FamilyMortgageLoansMember_53177d26-ad46-479d-b543-2fbdf02bf814" xlink:href="lkfn-20220930.xsd#lkfn_TotalConsumer1To4FamilyMortgageLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_7285ad7c-bccf-46c4-801f-2e2c4acd0a77" xlink:to="loc_lkfn_TotalConsumer1To4FamilyMortgageLoansMember_53177d26-ad46-479d-b543-2fbdf02bf814" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_f3a1ccc9-bc4e-4acf-b272-91b9199c8adb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_75804a36-2b80-4aca-abb5-59e349dcd6ae" xlink:to="loc_us-gaap_FinancialInstrumentAxis_f3a1ccc9-bc4e-4acf-b272-91b9199c8adb" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_f3a1ccc9-bc4e-4acf-b272-91b9199c8adb_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_f3a1ccc9-bc4e-4acf-b272-91b9199c8adb" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_f3a1ccc9-bc4e-4acf-b272-91b9199c8adb_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_f03fcaa4-dc2c-4005-a233-7ffd9b15544e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_f3a1ccc9-bc4e-4acf-b272-91b9199c8adb" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_f03fcaa4-dc2c-4005-a233-7ffd9b15544e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialLoanMember_a544d206-f046-4ee0-8948-3fa902a57e76" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommercialLoanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_f03fcaa4-dc2c-4005-a233-7ffd9b15544e" xlink:to="loc_us-gaap_CommercialLoanMember_a544d206-f046-4ee0-8948-3fa902a57e76" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditMember_06168e18-2a32-475c-a060-9ea5eb693258" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LineOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CommercialLoanMember_a544d206-f046-4ee0-8948-3fa902a57e76" xlink:to="loc_us-gaap_LineOfCreditMember_06168e18-2a32-475c-a060-9ea5eb693258" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_NonWorkingCapitalLoansMember_f2fa31d7-627c-4f36-ad9b-24784b1dae6d" xlink:href="lkfn-20220930.xsd#lkfn_NonWorkingCapitalLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CommercialLoanMember_a544d206-f046-4ee0-8948-3fa902a57e76" xlink:to="loc_lkfn_NonWorkingCapitalLoansMember_f2fa31d7-627c-4f36-ad9b-24784b1dae6d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialRealEstateMember_52239b5f-bd91-4e54-b722-80743928f873" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommercialRealEstateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CommercialLoanMember_a544d206-f046-4ee0-8948-3fa902a57e76" xlink:to="loc_us-gaap_CommercialRealEstateMember_52239b5f-bd91-4e54-b722-80743928f873" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_OwnerOccupiedLoansMember_fc29b67b-686b-4b6a-b738-8a1162f918fa" xlink:href="lkfn-20220930.xsd#lkfn_OwnerOccupiedLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CommercialLoanMember_a544d206-f046-4ee0-8948-3fa902a57e76" xlink:to="loc_lkfn_OwnerOccupiedLoansMember_fc29b67b-686b-4b6a-b738-8a1162f918fa" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_NonownerOccupiedLoansMember_3851fe37-5db6-442f-a1a2-c773a01a9d69" xlink:href="lkfn-20220930.xsd#lkfn_NonownerOccupiedLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CommercialLoanMember_a544d206-f046-4ee0-8948-3fa902a57e76" xlink:to="loc_lkfn_NonownerOccupiedLoansMember_3851fe37-5db6-442f-a1a2-c773a01a9d69" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_MultifamilyLoansMember_21b01c6e-97b3-48dc-bc6d-aee858ae9a76" xlink:href="lkfn-20220930.xsd#lkfn_MultifamilyLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CommercialLoanMember_a544d206-f046-4ee0-8948-3fa902a57e76" xlink:to="loc_lkfn_MultifamilyLoansMember_21b01c6e-97b3-48dc-bc6d-aee858ae9a76" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_LoansSecuredByFarmlandMember_bfabb41b-0b97-4593-8408-2a6301b0daea" xlink:href="lkfn-20220930.xsd#lkfn_LoansSecuredByFarmlandMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CommercialLoanMember_a544d206-f046-4ee0-8948-3fa902a57e76" xlink:to="loc_lkfn_LoansSecuredByFarmlandMember_bfabb41b-0b97-4593-8408-2a6301b0daea" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_LoansForAgriculturalProductionMember_eee7cae0-68b2-44a6-83c5-c7fe977cd4be" xlink:href="lkfn-20220930.xsd#lkfn_LoansForAgriculturalProductionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CommercialLoanMember_a544d206-f046-4ee0-8948-3fa902a57e76" xlink:to="loc_lkfn_LoansForAgriculturalProductionMember_eee7cae0-68b2-44a6-83c5-c7fe977cd4be" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_OtherCommercialLoansMember_a0567825-9081-44b8-8136-eb8593f2f3c5" xlink:href="lkfn-20220930.xsd#lkfn_OtherCommercialLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CommercialLoanMember_a544d206-f046-4ee0-8948-3fa902a57e76" xlink:to="loc_lkfn_OtherCommercialLoansMember_a0567825-9081-44b8-8136-eb8593f2f3c5" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConsumerLoanMember_377620be-78ff-4c70-b6cf-a053bda77f85" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConsumerLoanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_f03fcaa4-dc2c-4005-a233-7ffd9b15544e" xlink:to="loc_us-gaap_ConsumerLoanMember_377620be-78ff-4c70-b6cf-a053bda77f85" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FirstMortgageMember_7f0727ee-bf81-407f-bdc8-5f7f3944de56" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FirstMortgageMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConsumerLoanMember_377620be-78ff-4c70-b6cf-a053bda77f85" xlink:to="loc_us-gaap_FirstMortgageMember_7f0727ee-bf81-407f-bdc8-5f7f3944de56" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecondMortgageMember_5a412a3c-bc00-425e-8aee-8a4114365085" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SecondMortgageMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConsumerLoanMember_377620be-78ff-4c70-b6cf-a053bda77f85" xlink:to="loc_us-gaap_SecondMortgageMember_5a412a3c-bc00-425e-8aee-8a4114365085" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_ResidentialConstructionAndLandDevelopmentLoansMember_dc7dcb00-979d-42dd-89c6-94bc304d6e9e" xlink:href="lkfn-20220930.xsd#lkfn_ResidentialConstructionAndLandDevelopmentLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConsumerLoanMember_377620be-78ff-4c70-b6cf-a053bda77f85" xlink:to="loc_lkfn_ResidentialConstructionAndLandDevelopmentLoansMember_dc7dcb00-979d-42dd-89c6-94bc304d6e9e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_OtherConsumerLoansMember_80ede27c-6313-48d0-8fb9-5db5f632799c" xlink:href="lkfn-20220930.xsd#lkfn_OtherConsumerLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConsumerLoanMember_377620be-78ff-4c70-b6cf-a053bda77f85" xlink:to="loc_lkfn_OtherConsumerLoansMember_80ede27c-6313-48d0-8fb9-5db5f632799c" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_b9283ebd-a884-465f-a882-7152e8ca013c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivablePortfolioSegmentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAccountsNotesLoansAndFinancingReceivableTable_75804a36-2b80-4aca-abb5-59e349dcd6ae" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_b9283ebd-a884-465f-a882-7152e8ca013c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_b9283ebd-a884-465f-a882-7152e8ca013c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_b9283ebd-a884-465f-a882-7152e8ca013c" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_b9283ebd-a884-465f-a882-7152e8ca013c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_b707fbb6-cb81-4049-9fbb-039e679c2305" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_b9283ebd-a884-465f-a882-7152e8ca013c" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_b707fbb6-cb81-4049-9fbb-039e679c2305" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialPortfolioSegmentMember_1338bb55-e098-492b-8590-6998ef1672e5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommercialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_b707fbb6-cb81-4049-9fbb-039e679c2305" xlink:to="loc_us-gaap_CommercialPortfolioSegmentMember_1338bb55-e098-492b-8590-6998ef1672e5" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.lakeland.com/role/ALLOWANCEFORCREDITLOSSESANDCREDITQUALITYAllowanceforloanlossesbyportfoliosegmentDetails" xlink:type="simple" xlink:href="lkfn-20220930.xsd#ALLOWANCEFORCREDITLOSSESANDCREDITQUALITYAllowanceforloanlossesbyportfoliosegmentDetails"/>
  <link:definitionLink xlink:role="http://www.lakeland.com/role/ALLOWANCEFORCREDITLOSSESANDCREDITQUALITYAllowanceforloanlossesbyportfoliosegmentDetails" xlink:type="extended" id="i6a0f91af893940ba8a18202b8f0d14ff_ALLOWANCEFORCREDITLOSSESANDCREDITQUALITYAllowanceforloanlossesbyportfoliosegmentDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_732fddbc-d1a7-434d-b0b2-8cb30f426e4a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableAllowanceForCreditLossExcludingAccruedInterest_1b2fa0bf-31ca-45f7-ac1d-b5844db03a1a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableAllowanceForCreditLossExcludingAccruedInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_732fddbc-d1a7-434d-b0b2-8cb30f426e4a" xlink:to="loc_us-gaap_FinancingReceivableAllowanceForCreditLossExcludingAccruedInterest_1b2fa0bf-31ca-45f7-ac1d-b5844db03a1a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestCreditLossExpenseReversal_745cced7-1072-4046-82c8-9ee544f3f80f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestCreditLossExpenseReversal"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_732fddbc-d1a7-434d-b0b2-8cb30f426e4a" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestCreditLossExpenseReversal_745cced7-1072-4046-82c8-9ee544f3f80f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoff_43e5288e-0d9a-4b66-ad89-31c1621515f2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoff"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_732fddbc-d1a7-434d-b0b2-8cb30f426e4a" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoff_43e5288e-0d9a-4b66-ad89-31c1621515f2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossRecovery_519103f1-ab26-4ee4-896c-2e797edc59f6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossRecovery"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_732fddbc-d1a7-434d-b0b2-8cb30f426e4a" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossRecovery_519103f1-ab26-4ee4-896c-2e797edc59f6" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoffAfterRecovery_2c6ea21b-9d59-4909-be33-891ca0e29804" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoffAfterRecovery"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_732fddbc-d1a7-434d-b0b2-8cb30f426e4a" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoffAfterRecovery_2c6ea21b-9d59-4909-be33-891ca0e29804" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableAllowanceForCreditLossExcludingAccruedInterest_276345af-7a93-44e4-baa0-4516f79e0483" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableAllowanceForCreditLossExcludingAccruedInterest"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable_8812f803-cebf-4d50-a41e-5a39acce3331" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_732fddbc-d1a7-434d-b0b2-8cb30f426e4a" xlink:to="loc_us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable_8812f803-cebf-4d50-a41e-5a39acce3331" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_e7cdc181-2f63-4443-aa95-3d1e3a5e2643" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivablePortfolioSegmentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable_8812f803-cebf-4d50-a41e-5a39acce3331" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_e7cdc181-2f63-4443-aa95-3d1e3a5e2643" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_e7cdc181-2f63-4443-aa95-3d1e3a5e2643_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_e7cdc181-2f63-4443-aa95-3d1e3a5e2643" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_e7cdc181-2f63-4443-aa95-3d1e3a5e2643_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_ac2a1798-6902-431a-9efc-71ab81887854" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_e7cdc181-2f63-4443-aa95-3d1e3a5e2643" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_ac2a1798-6902-431a-9efc-71ab81887854" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialPortfolioSegmentMember_3319a3a6-c16f-46ff-8503-b1a53c7d954c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommercialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_ac2a1798-6902-431a-9efc-71ab81887854" xlink:to="loc_us-gaap_CommercialPortfolioSegmentMember_3319a3a6-c16f-46ff-8503-b1a53c7d954c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConsumerPortfolioSegmentMember_7ba55c47-a8cb-43a4-bd64-201c36834469" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConsumerPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_ac2a1798-6902-431a-9efc-71ab81887854" xlink:to="loc_us-gaap_ConsumerPortfolioSegmentMember_7ba55c47-a8cb-43a4-bd64-201c36834469" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_e5a9ff2b-94d0-4bc6-91a5-5fe18f1fa324" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable_8812f803-cebf-4d50-a41e-5a39acce3331" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_e5a9ff2b-94d0-4bc6-91a5-5fe18f1fa324" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_e5a9ff2b-94d0-4bc6-91a5-5fe18f1fa324_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_e5a9ff2b-94d0-4bc6-91a5-5fe18f1fa324" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_e5a9ff2b-94d0-4bc6-91a5-5fe18f1fa324_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_02ab7de9-8f61-4d40-b236-ab87b927769a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_e5a9ff2b-94d0-4bc6-91a5-5fe18f1fa324" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_02ab7de9-8f61-4d40-b236-ab87b927769a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_TotalCommercialAndIndustrialLoansMember_1a69cacb-5952-4951-8ec9-bc908acaa597" xlink:href="lkfn-20220930.xsd#lkfn_TotalCommercialAndIndustrialLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_02ab7de9-8f61-4d40-b236-ab87b927769a" xlink:to="loc_lkfn_TotalCommercialAndIndustrialLoansMember_1a69cacb-5952-4951-8ec9-bc908acaa597" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialRealEstatePortfolioSegmentMember_7866e72d-2080-4dcf-8a3a-202c0a7dbcad" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommercialRealEstatePortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_02ab7de9-8f61-4d40-b236-ab87b927769a" xlink:to="loc_us-gaap_CommercialRealEstatePortfolioSegmentMember_7866e72d-2080-4dcf-8a3a-202c0a7dbcad" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_AgriBusinessAndAgriculturalLoansMember_3d5933b2-2ef5-48a1-82b2-b15b3d3fddab" xlink:href="lkfn-20220930.xsd#lkfn_AgriBusinessAndAgriculturalLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_02ab7de9-8f61-4d40-b236-ab87b927769a" xlink:to="loc_lkfn_AgriBusinessAndAgriculturalLoansMember_3d5933b2-2ef5-48a1-82b2-b15b3d3fddab" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_OtherCommercialMember_97010d20-ad0d-418f-b0b1-ecc79537ef29" xlink:href="lkfn-20220930.xsd#lkfn_OtherCommercialMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_02ab7de9-8f61-4d40-b236-ab87b927769a" xlink:to="loc_lkfn_OtherCommercialMember_97010d20-ad0d-418f-b0b1-ecc79537ef29" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResidentialPortfolioSegmentMember_2cf85faf-28b1-4f87-bb20-a645e61c1ac2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ResidentialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_02ab7de9-8f61-4d40-b236-ab87b927769a" xlink:to="loc_us-gaap_ResidentialPortfolioSegmentMember_2cf85faf-28b1-4f87-bb20-a645e61c1ac2" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_OtherConsumerMember_f92baeff-63cb-4be4-ab38-df745905082e" xlink:href="lkfn-20220930.xsd#lkfn_OtherConsumerMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_02ab7de9-8f61-4d40-b236-ab87b927769a" xlink:to="loc_lkfn_OtherConsumerMember_f92baeff-63cb-4be4-ab38-df745905082e" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_UnallocatedMember_2e5334f8-c3fd-4177-b3cf-88fb72e64fa2" xlink:href="lkfn-20220930.xsd#lkfn_UnallocatedMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_02ab7de9-8f61-4d40-b236-ab87b927769a" xlink:to="loc_lkfn_UnallocatedMember_2e5334f8-c3fd-4177-b3cf-88fb72e64fa2" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CumulativeEffectPeriodOfAdoptionAxis_0d4097d5-820f-4a8c-93fa-c2f36d4efe87" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_CumulativeEffectPeriodOfAdoptionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable_8812f803-cebf-4d50-a41e-5a39acce3331" xlink:to="loc_srt_CumulativeEffectPeriodOfAdoptionAxis_0d4097d5-820f-4a8c-93fa-c2f36d4efe87" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_0d4097d5-820f-4a8c-93fa-c2f36d4efe87_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_CumulativeEffectPeriodOfAdoptionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CumulativeEffectPeriodOfAdoptionAxis_0d4097d5-820f-4a8c-93fa-c2f36d4efe87" xlink:to="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_0d4097d5-820f-4a8c-93fa-c2f36d4efe87_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_7c92bfdd-81d5-4919-bf8b-75992d8de513" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_CumulativeEffectPeriodOfAdoptionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CumulativeEffectPeriodOfAdoptionAxis_0d4097d5-820f-4a8c-93fa-c2f36d4efe87" xlink:to="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_7c92bfdd-81d5-4919-bf8b-75992d8de513" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CumulativeEffectPeriodOfAdoptionAdjustmentMember_40b06713-53d1-4884-ad32-fb860d309a5e" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_CumulativeEffectPeriodOfAdoptionAdjustmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_CumulativeEffectPeriodOfAdoptionDomain_7c92bfdd-81d5-4919-bf8b-75992d8de513" xlink:to="loc_srt_CumulativeEffectPeriodOfAdoptionAdjustmentMember_40b06713-53d1-4884-ad32-fb860d309a5e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsForNewAccountingPronouncementsAxis_c35838f3-8378-4087-90d8-abea45d679bb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AdjustmentsForNewAccountingPronouncementsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable_8812f803-cebf-4d50-a41e-5a39acce3331" xlink:to="loc_us-gaap_AdjustmentsForNewAccountingPronouncementsAxis_c35838f3-8378-4087-90d8-abea45d679bb" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TypeOfAdoptionMember_c35838f3-8378-4087-90d8-abea45d679bb_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TypeOfAdoptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AdjustmentsForNewAccountingPronouncementsAxis_c35838f3-8378-4087-90d8-abea45d679bb" xlink:to="loc_us-gaap_TypeOfAdoptionMember_c35838f3-8378-4087-90d8-abea45d679bb_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TypeOfAdoptionMember_42832c76-90fa-4d0c-b502-f68c64bf3372" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TypeOfAdoptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AdjustmentsForNewAccountingPronouncementsAxis_c35838f3-8378-4087-90d8-abea45d679bb" xlink:to="loc_us-gaap_TypeOfAdoptionMember_42832c76-90fa-4d0c-b502-f68c64bf3372" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountingStandardsUpdate201613Member_1a3f302c-cdc7-4ab0-9c9a-bdfee8539043" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccountingStandardsUpdate201613Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TypeOfAdoptionMember_42832c76-90fa-4d0c-b502-f68c64bf3372" xlink:to="loc_us-gaap_AccountingStandardsUpdate201613Member_1a3f302c-cdc7-4ab0-9c9a-bdfee8539043" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.lakeland.com/role/ALLOWANCEFORCREDITLOSSESANDCREDITQUALITYAdditionalInformationDetails" xlink:type="simple" xlink:href="lkfn-20220930.xsd#ALLOWANCEFORCREDITLOSSESANDCREDITQUALITYAdditionalInformationDetails"/>
  <link:definitionLink xlink:role="http://www.lakeland.com/role/ALLOWANCEFORCREDITLOSSESANDCREDITQUALITYAdditionalInformationDetails" xlink:type="extended" id="i46c282a2b42b42548ad0434567a8a067_ALLOWANCEFORCREDITLOSSESANDCREDITQUALITYAdditionalInformationDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_7b2acc78-0b7f-446e-822e-5ef78c91dc09" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_LoanAmountOfCreditQualityAnalysis_9287acb1-42cb-41e1-a764-669b21711ecd" xlink:href="lkfn-20220930.xsd#lkfn_LoanAmountOfCreditQualityAnalysis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_7b2acc78-0b7f-446e-822e-5ef78c91dc09" xlink:to="loc_lkfn_LoanAmountOfCreditQualityAnalysis_9287acb1-42cb-41e1-a764-669b21711ecd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestBeforeAllowanceForCreditLoss_06071cb4-7316-4560-8eb7-ccc8154cdeba" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestBeforeAllowanceForCreditLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_7b2acc78-0b7f-446e-822e-5ef78c91dc09" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestBeforeAllowanceForCreditLoss_06071cb4-7316-4560-8eb7-ccc8154cdeba" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableModificationsRecordedInvestment_37502e74-5f1b-4d06-80e6-82c874ffbdb9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableModificationsRecordedInvestment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_7b2acc78-0b7f-446e-822e-5ef78c91dc09" xlink:to="loc_us-gaap_FinancingReceivableModificationsRecordedInvestment_37502e74-5f1b-4d06-80e6-82c874ffbdb9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableModificationsNumberOfContracts2_932a64fe-5228-4dc9-b745-551512917ada" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableModificationsNumberOfContracts2"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_7b2acc78-0b7f-446e-822e-5ef78c91dc09" xlink:to="loc_us-gaap_FinancingReceivableModificationsNumberOfContracts2_932a64fe-5228-4dc9-b745-551512917ada" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable_3de3b031-1e4b-4eb9-a504-6fed16e40c50" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_7b2acc78-0b7f-446e-822e-5ef78c91dc09" xlink:to="loc_us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable_3de3b031-1e4b-4eb9-a504-6fed16e40c50" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_56f39579-d6df-4c95-94fa-9387dbf01d59" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable_3de3b031-1e4b-4eb9-a504-6fed16e40c50" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_56f39579-d6df-4c95-94fa-9387dbf01d59" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_56f39579-d6df-4c95-94fa-9387dbf01d59_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_56f39579-d6df-4c95-94fa-9387dbf01d59" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_56f39579-d6df-4c95-94fa-9387dbf01d59_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_f0f637b9-1056-484c-9d92-3444d6cee1c0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_56f39579-d6df-4c95-94fa-9387dbf01d59" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_f0f637b9-1056-484c-9d92-3444d6cee1c0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_PayCheckProtectionProgramMember_b2ea8bbb-2018-47b1-bdc6-f9c32a1769ef" xlink:href="lkfn-20220930.xsd#lkfn_PayCheckProtectionProgramMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_f0f637b9-1056-484c-9d92-3444d6cee1c0" xlink:to="loc_lkfn_PayCheckProtectionProgramMember_b2ea8bbb-2018-47b1-bdc6-f9c32a1769ef" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConsumerLoanMember_11d1d2cf-349a-475a-a359-ab5051cbb6a1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ConsumerLoanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_f0f637b9-1056-484c-9d92-3444d6cee1c0" xlink:to="loc_us-gaap_ConsumerLoanMember_11d1d2cf-349a-475a-a359-ab5051cbb6a1" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.lakeland.com/role/ALLOWANCEFORCREDITLOSSESANDCREDITQUALITYRiskcategoryofloansbyloansegmentandoriginationDetails" xlink:type="simple" xlink:href="lkfn-20220930.xsd#ALLOWANCEFORCREDITLOSSESANDCREDITQUALITYRiskcategoryofloansbyloansegmentandoriginationDetails"/>
  <link:definitionLink xlink:role="http://www.lakeland.com/role/ALLOWANCEFORCREDITLOSSESANDCREDITQUALITYRiskcategoryofloansbyloansegmentandoriginationDetails" xlink:type="extended" id="id9d0f984dd3c4f309777abfa6bbb7288_ALLOWANCEFORCREDITLOSSESANDCREDITQUALITYRiskcategoryofloansbyloansegmentandoriginationDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_0a1488b3-698e-47b1-a3fe-2b20b6ff0551" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableRecordedInvestmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearOneOriginatedCurrentFiscalYear_4bd8cf06-9da7-49b7-adea-1b85b5354d5c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestYearOneOriginatedCurrentFiscalYear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_0a1488b3-698e-47b1-a3fe-2b20b6ff0551" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearOneOriginatedCurrentFiscalYear_4bd8cf06-9da7-49b7-adea-1b85b5354d5c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearTwoOriginatedFiscalYearBeforeCurrentFiscalYear_282cc911-0f2b-4c39-b9cb-df589f80e1c9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestYearTwoOriginatedFiscalYearBeforeCurrentFiscalYear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_0a1488b3-698e-47b1-a3fe-2b20b6ff0551" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearTwoOriginatedFiscalYearBeforeCurrentFiscalYear_282cc911-0f2b-4c39-b9cb-df589f80e1c9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearThreeOriginatedTwoYearsBeforeCurrentFiscalYear_e9db7588-6a34-44c2-aa28-603ef971c4b5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestYearThreeOriginatedTwoYearsBeforeCurrentFiscalYear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_0a1488b3-698e-47b1-a3fe-2b20b6ff0551" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearThreeOriginatedTwoYearsBeforeCurrentFiscalYear_e9db7588-6a34-44c2-aa28-603ef971c4b5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearFourOriginatedThreeYearsBeforeCurrentFiscalYear_81fb47da-66a2-477b-b9b2-85f6be10b641" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestYearFourOriginatedThreeYearsBeforeCurrentFiscalYear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_0a1488b3-698e-47b1-a3fe-2b20b6ff0551" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearFourOriginatedThreeYearsBeforeCurrentFiscalYear_81fb47da-66a2-477b-b9b2-85f6be10b641" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearFiveOriginatedFourYearsBeforeCurrentFiscalYear_ff429409-c653-4e97-9983-f3cb1d2a28b8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestYearFiveOriginatedFourYearsBeforeCurrentFiscalYear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_0a1488b3-698e-47b1-a3fe-2b20b6ff0551" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearFiveOriginatedFourYearsBeforeCurrentFiscalYear_ff429409-c653-4e97-9983-f3cb1d2a28b8" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestOriginatedMoreThanFiveYearsBeforeCurrentFiscalYear_9ec3a0b8-6330-419b-8db2-886fe41896c0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestOriginatedMoreThanFiveYearsBeforeCurrentFiscalYear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_0a1488b3-698e-47b1-a3fe-2b20b6ff0551" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestOriginatedMoreThanFiveYearsBeforeCurrentFiscalYear_9ec3a0b8-6330-419b-8db2-886fe41896c0" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestRevolvingConvertedToTermLoan_a5048e12-28a2-4226-b312-a09a39e556be" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestRevolvingConvertedToTermLoan"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_0a1488b3-698e-47b1-a3fe-2b20b6ff0551" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestRevolvingConvertedToTermLoan_a5048e12-28a2-4226-b312-a09a39e556be" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestRevolving_7bf43203-b409-4624-8930-a70a1add914a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestRevolving"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_0a1488b3-698e-47b1-a3fe-2b20b6ff0551" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestRevolving_7bf43203-b409-4624-8930-a70a1add914a" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestBeforeAllowanceForCreditLoss_79255dde-262c-4b62-ac19-0a920637bc38" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestBeforeAllowanceForCreditLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_0a1488b3-698e-47b1-a3fe-2b20b6ff0551" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestBeforeAllowanceForCreditLoss_79255dde-262c-4b62-ac19-0a920637bc38" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_7a163a47-00e0-41d5-addf-028041a1cf75" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_0a1488b3-698e-47b1-a3fe-2b20b6ff0551" xlink:to="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_7a163a47-00e0-41d5-addf-028041a1cf75" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InternalCreditAssessmentAxis_c9d87cd5-f0ab-4d33-913e-a1ad619a6ac1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InternalCreditAssessmentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_7a163a47-00e0-41d5-addf-028041a1cf75" xlink:to="loc_us-gaap_InternalCreditAssessmentAxis_c9d87cd5-f0ab-4d33-913e-a1ad619a6ac1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InternalCreditAssessmentDomain_c9d87cd5-f0ab-4d33-913e-a1ad619a6ac1_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InternalCreditAssessmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_InternalCreditAssessmentAxis_c9d87cd5-f0ab-4d33-913e-a1ad619a6ac1" xlink:to="loc_us-gaap_InternalCreditAssessmentDomain_c9d87cd5-f0ab-4d33-913e-a1ad619a6ac1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InternalCreditAssessmentDomain_91205ced-7551-4e27-a40f-c3cb5941038d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InternalCreditAssessmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_InternalCreditAssessmentAxis_c9d87cd5-f0ab-4d33-913e-a1ad619a6ac1" xlink:to="loc_us-gaap_InternalCreditAssessmentDomain_91205ced-7551-4e27-a40f-c3cb5941038d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PassMember_6d8d92f7-cef2-4999-80e3-e939f10fc20d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PassMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InternalCreditAssessmentDomain_91205ced-7551-4e27-a40f-c3cb5941038d" xlink:to="loc_us-gaap_PassMember_6d8d92f7-cef2-4999-80e3-e939f10fc20d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SpecialMentionMember_1736b546-1fc9-4f98-b73e-ea60eb4ba713" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SpecialMentionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InternalCreditAssessmentDomain_91205ced-7551-4e27-a40f-c3cb5941038d" xlink:to="loc_us-gaap_SpecialMentionMember_1736b546-1fc9-4f98-b73e-ea60eb4ba713" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubstandardMember_bdd5ebbb-2a13-4463-9917-cc88585839fb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SubstandardMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InternalCreditAssessmentDomain_91205ced-7551-4e27-a40f-c3cb5941038d" xlink:to="loc_us-gaap_SubstandardMember_bdd5ebbb-2a13-4463-9917-cc88585839fb" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonperformingFinancingReceivableMember_bc0d181c-ebcd-4995-9a29-dd3288a5f1cc" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NonperformingFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InternalCreditAssessmentDomain_91205ced-7551-4e27-a40f-c3cb5941038d" xlink:to="loc_us-gaap_NonperformingFinancingReceivableMember_bc0d181c-ebcd-4995-9a29-dd3288a5f1cc" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_21186280-dba4-4b21-9199-97bc2e50e3d9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivablePortfolioSegmentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_7a163a47-00e0-41d5-addf-028041a1cf75" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_21186280-dba4-4b21-9199-97bc2e50e3d9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_21186280-dba4-4b21-9199-97bc2e50e3d9_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_21186280-dba4-4b21-9199-97bc2e50e3d9" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_21186280-dba4-4b21-9199-97bc2e50e3d9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_49b852bf-a149-4e42-9708-2c781c4687d4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_21186280-dba4-4b21-9199-97bc2e50e3d9" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_49b852bf-a149-4e42-9708-2c781c4687d4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditMember_aa9d163b-f046-411f-a018-0880ed4b0ef4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LineOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_49b852bf-a149-4e42-9708-2c781c4687d4" xlink:to="loc_us-gaap_LineOfCreditMember_aa9d163b-f046-411f-a018-0880ed4b0ef4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_NonWorkingCapitalLoansMember_a6ab24d1-1692-4869-8347-118aff8c18cd" xlink:href="lkfn-20220930.xsd#lkfn_NonWorkingCapitalLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_49b852bf-a149-4e42-9708-2c781c4687d4" xlink:to="loc_lkfn_NonWorkingCapitalLoansMember_a6ab24d1-1692-4869-8347-118aff8c18cd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialRealEstateMember_f1dd6b9f-74a5-4cf3-aa39-d595d1dff805" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommercialRealEstateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_49b852bf-a149-4e42-9708-2c781c4687d4" xlink:to="loc_us-gaap_CommercialRealEstateMember_f1dd6b9f-74a5-4cf3-aa39-d595d1dff805" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_OwnerOccupiedLoansMember_87f1f088-48c9-4a54-930b-01cea93b3a3b" xlink:href="lkfn-20220930.xsd#lkfn_OwnerOccupiedLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_49b852bf-a149-4e42-9708-2c781c4687d4" xlink:to="loc_lkfn_OwnerOccupiedLoansMember_87f1f088-48c9-4a54-930b-01cea93b3a3b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_NonownerOccupiedLoansMember_620875d2-4908-43c5-8db5-b48be12afe0b" xlink:href="lkfn-20220930.xsd#lkfn_NonownerOccupiedLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_49b852bf-a149-4e42-9708-2c781c4687d4" xlink:to="loc_lkfn_NonownerOccupiedLoansMember_620875d2-4908-43c5-8db5-b48be12afe0b" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_MultifamilyLoansMember_cbf5d498-aa68-485b-97b9-9c781649f969" xlink:href="lkfn-20220930.xsd#lkfn_MultifamilyLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_49b852bf-a149-4e42-9708-2c781c4687d4" xlink:to="loc_lkfn_MultifamilyLoansMember_cbf5d498-aa68-485b-97b9-9c781649f969" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_LoansSecuredByFarmlandMember_e233568f-38d8-433f-9aed-cf5b1407772a" xlink:href="lkfn-20220930.xsd#lkfn_LoansSecuredByFarmlandMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_49b852bf-a149-4e42-9708-2c781c4687d4" xlink:to="loc_lkfn_LoansSecuredByFarmlandMember_e233568f-38d8-433f-9aed-cf5b1407772a" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_LoansForAgriculturalProductionMember_48115622-790f-4dd3-be01-63d5eae6505b" xlink:href="lkfn-20220930.xsd#lkfn_LoansForAgriculturalProductionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_49b852bf-a149-4e42-9708-2c781c4687d4" xlink:to="loc_lkfn_LoansForAgriculturalProductionMember_48115622-790f-4dd3-be01-63d5eae6505b" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_OtherCommercialLoansMember_8e07eccc-bf5d-442c-aa0e-b0acb40127f5" xlink:href="lkfn-20220930.xsd#lkfn_OtherCommercialLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_49b852bf-a149-4e42-9708-2c781c4687d4" xlink:to="loc_lkfn_OtherCommercialLoansMember_8e07eccc-bf5d-442c-aa0e-b0acb40127f5" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FirstMortgageMember_5e096414-bdaa-4fe6-a705-252e7ec01fce" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FirstMortgageMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_49b852bf-a149-4e42-9708-2c781c4687d4" xlink:to="loc_us-gaap_FirstMortgageMember_5e096414-bdaa-4fe6-a705-252e7ec01fce" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecondMortgageMember_fd9f42ae-fd2c-4943-8bdf-ffcf72ce992c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SecondMortgageMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_49b852bf-a149-4e42-9708-2c781c4687d4" xlink:to="loc_us-gaap_SecondMortgageMember_fd9f42ae-fd2c-4943-8bdf-ffcf72ce992c" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResidentialMortgageMember_2bd1f9ef-5521-4901-8fec-7469c11ed29f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ResidentialMortgageMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_49b852bf-a149-4e42-9708-2c781c4687d4" xlink:to="loc_us-gaap_ResidentialMortgageMember_2bd1f9ef-5521-4901-8fec-7469c11ed29f" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_OtherConsumerLoansMember_541e3be6-b6f6-48c8-af99-2b00339cfbd7" xlink:href="lkfn-20220930.xsd#lkfn_OtherConsumerLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_49b852bf-a149-4e42-9708-2c781c4687d4" xlink:to="loc_lkfn_OtherConsumerLoansMember_541e3be6-b6f6-48c8-af99-2b00339cfbd7" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_04651650-7748-41d2-b965-ab5fda4e1347" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_7a163a47-00e0-41d5-addf-028041a1cf75" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_04651650-7748-41d2-b965-ab5fda4e1347" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_04651650-7748-41d2-b965-ab5fda4e1347_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_04651650-7748-41d2-b965-ab5fda4e1347" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_04651650-7748-41d2-b965-ab5fda4e1347_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_ab1fe988-ed5f-452a-91eb-f3a40c93d256" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_04651650-7748-41d2-b965-ab5fda4e1347" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_ab1fe988-ed5f-452a-91eb-f3a40c93d256" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_TotalCommercialAndIndustrialLoansMember_449211be-dcc8-4d3b-8145-b50c356351bd" xlink:href="lkfn-20220930.xsd#lkfn_TotalCommercialAndIndustrialLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_ab1fe988-ed5f-452a-91eb-f3a40c93d256" xlink:to="loc_lkfn_TotalCommercialAndIndustrialLoansMember_449211be-dcc8-4d3b-8145-b50c356351bd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialRealEstatePortfolioSegmentMember_5e93638e-c4bd-40a7-bfae-dd54a038bb66" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommercialRealEstatePortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_ab1fe988-ed5f-452a-91eb-f3a40c93d256" xlink:to="loc_us-gaap_CommercialRealEstatePortfolioSegmentMember_5e93638e-c4bd-40a7-bfae-dd54a038bb66" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_AgriBusinessAndAgriculturalLoansMember_eaaf4749-3775-4fd5-964c-41d40a59baf7" xlink:href="lkfn-20220930.xsd#lkfn_AgriBusinessAndAgriculturalLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_ab1fe988-ed5f-452a-91eb-f3a40c93d256" xlink:to="loc_lkfn_AgriBusinessAndAgriculturalLoansMember_eaaf4749-3775-4fd5-964c-41d40a59baf7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_OtherCommercialLoansMember_4f6bf2d0-f398-4f35-8ca0-63c369f17aa3" xlink:href="lkfn-20220930.xsd#lkfn_OtherCommercialLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_ab1fe988-ed5f-452a-91eb-f3a40c93d256" xlink:to="loc_lkfn_OtherCommercialLoansMember_4f6bf2d0-f398-4f35-8ca0-63c369f17aa3" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResidentialPortfolioSegmentMember_06f58b67-aaf1-4f57-aae1-29120ccb87d7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ResidentialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_ab1fe988-ed5f-452a-91eb-f3a40c93d256" xlink:to="loc_us-gaap_ResidentialPortfolioSegmentMember_06f58b67-aaf1-4f57-aae1-29120ccb87d7" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_PayCheckProtectionProgramMember_28ddde76-3d82-45e7-bee0-6111d1dd737b" xlink:href="lkfn-20220930.xsd#lkfn_PayCheckProtectionProgramMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_ab1fe988-ed5f-452a-91eb-f3a40c93d256" xlink:to="loc_lkfn_PayCheckProtectionProgramMember_28ddde76-3d82-45e7-bee0-6111d1dd737b" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.lakeland.com/role/ALLOWANCEFORCREDITLOSSESANDCREDITQUALITYAgingOfTheAmortizedCostInPastDueLoansDetails" xlink:type="simple" xlink:href="lkfn-20220930.xsd#ALLOWANCEFORCREDITLOSSESANDCREDITQUALITYAgingOfTheAmortizedCostInPastDueLoansDetails"/>
  <link:definitionLink xlink:role="http://www.lakeland.com/role/ALLOWANCEFORCREDITLOSSESANDCREDITQUALITYAgingOfTheAmortizedCostInPastDueLoansDetails" xlink:type="extended" id="i6ac8e182256940218ebb543cb602c51b_ALLOWANCEFORCREDITLOSSESANDCREDITQUALITYAgingOfTheAmortizedCostInPastDueLoansDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_3173fbab-8c7d-4759-b6c5-e7ff26c1ac2d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_FinancingReceivableAmortizedCostAgingAbstract_ef81ff77-899b-4bf3-b65e-bf24132b82b1" xlink:href="lkfn-20220930.xsd#lkfn_FinancingReceivableAmortizedCostAgingAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_3173fbab-8c7d-4759-b6c5-e7ff26c1ac2d" xlink:to="loc_lkfn_FinancingReceivableAmortizedCostAgingAbstract_ef81ff77-899b-4bf3-b65e-bf24132b82b1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_FinancingReceivableAmortizedCostBeforeAllowanceForCreditLossExcludingAccruedInterest_8cc6b366-1167-4c3b-bba7-7b072224b111" xlink:href="lkfn-20220930.xsd#lkfn_FinancingReceivableAmortizedCostBeforeAllowanceForCreditLossExcludingAccruedInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_FinancingReceivableAmortizedCostAgingAbstract_ef81ff77-899b-4bf3-b65e-bf24132b82b1" xlink:to="loc_lkfn_FinancingReceivableAmortizedCostBeforeAllowanceForCreditLossExcludingAccruedInterest_8cc6b366-1167-4c3b-bba7-7b072224b111" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_FinancingReceivableAmortizedCost90DaysOrMorePastDueStillAccruing_d2eef6de-4f9b-448b-94c8-8f695dd0bcff" xlink:href="lkfn-20220930.xsd#lkfn_FinancingReceivableAmortizedCost90DaysOrMorePastDueStillAccruing"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_FinancingReceivableAmortizedCostAgingAbstract_ef81ff77-899b-4bf3-b65e-bf24132b82b1" xlink:to="loc_lkfn_FinancingReceivableAmortizedCost90DaysOrMorePastDueStillAccruing_d2eef6de-4f9b-448b-94c8-8f695dd0bcff" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_FinancingReceivableAmortizedCostNonaccrual_acc23f67-4799-4607-b231-31f286ce1c77" xlink:href="lkfn-20220930.xsd#lkfn_FinancingReceivableAmortizedCostNonaccrual"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_FinancingReceivableAmortizedCostAgingAbstract_ef81ff77-899b-4bf3-b65e-bf24132b82b1" xlink:to="loc_lkfn_FinancingReceivableAmortizedCostNonaccrual_acc23f67-4799-4607-b231-31f286ce1c77" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_FinancingReceivableAmortizedCostNonaccrualWithNoAllowanceOfCreditLoss_0b7673b9-fff1-4688-8dba-29a98641dc21" xlink:href="lkfn-20220930.xsd#lkfn_FinancingReceivableAmortizedCostNonaccrualWithNoAllowanceOfCreditLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_FinancingReceivableAmortizedCostAgingAbstract_ef81ff77-899b-4bf3-b65e-bf24132b82b1" xlink:to="loc_lkfn_FinancingReceivableAmortizedCostNonaccrualWithNoAllowanceOfCreditLoss_0b7673b9-fff1-4688-8dba-29a98641dc21" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable_7fea3fb7-e5c6-478f-838f-0c76ac365901" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_3173fbab-8c7d-4759-b6c5-e7ff26c1ac2d" xlink:to="loc_us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable_7fea3fb7-e5c6-478f-838f-0c76ac365901" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_831cdc53-dc54-4397-8a9c-522daa4e833c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable_7fea3fb7-e5c6-478f-838f-0c76ac365901" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_831cdc53-dc54-4397-8a9c-522daa4e833c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_831cdc53-dc54-4397-8a9c-522daa4e833c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_831cdc53-dc54-4397-8a9c-522daa4e833c" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_831cdc53-dc54-4397-8a9c-522daa4e833c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_38065372-48a8-417b-8372-fa165494d04a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_831cdc53-dc54-4397-8a9c-522daa4e833c" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_38065372-48a8-417b-8372-fa165494d04a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_TotalCommercialAndIndustrialLoansMember_62d5c718-5501-4e52-8cc4-b29dc8560131" xlink:href="lkfn-20220930.xsd#lkfn_TotalCommercialAndIndustrialLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_38065372-48a8-417b-8372-fa165494d04a" xlink:to="loc_lkfn_TotalCommercialAndIndustrialLoansMember_62d5c718-5501-4e52-8cc4-b29dc8560131" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialRealEstatePortfolioSegmentMember_d0d77f35-b3c2-4383-8a94-0fba1fc3b6e7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommercialRealEstatePortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_38065372-48a8-417b-8372-fa165494d04a" xlink:to="loc_us-gaap_CommercialRealEstatePortfolioSegmentMember_d0d77f35-b3c2-4383-8a94-0fba1fc3b6e7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_AgriBusinessAndAgriculturalLoansMember_e3eaabc9-a967-40df-a3ac-26227215c18a" xlink:href="lkfn-20220930.xsd#lkfn_AgriBusinessAndAgriculturalLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_38065372-48a8-417b-8372-fa165494d04a" xlink:to="loc_lkfn_AgriBusinessAndAgriculturalLoansMember_e3eaabc9-a967-40df-a3ac-26227215c18a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResidentialPortfolioSegmentMember_6e72a895-03e0-478e-8bd8-11d35dad7c3d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ResidentialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_38065372-48a8-417b-8372-fa165494d04a" xlink:to="loc_us-gaap_ResidentialPortfolioSegmentMember_6e72a895-03e0-478e-8bd8-11d35dad7c3d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_f2ac1d19-0b35-4d6b-9935-1534cf235471" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivablePortfolioSegmentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable_7fea3fb7-e5c6-478f-838f-0c76ac365901" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_f2ac1d19-0b35-4d6b-9935-1534cf235471" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_f2ac1d19-0b35-4d6b-9935-1534cf235471_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_f2ac1d19-0b35-4d6b-9935-1534cf235471" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_f2ac1d19-0b35-4d6b-9935-1534cf235471_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_e001493c-a361-4d36-86f5-340b38eed650" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_f2ac1d19-0b35-4d6b-9935-1534cf235471" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_e001493c-a361-4d36-86f5-340b38eed650" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditMember_6c4c55fe-25fc-40d5-969e-39987896bc27" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LineOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_e001493c-a361-4d36-86f5-340b38eed650" xlink:to="loc_us-gaap_LineOfCreditMember_6c4c55fe-25fc-40d5-969e-39987896bc27" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_NonWorkingCapitalLoansMember_9bacba89-c896-4f96-84dc-af16d949eb59" xlink:href="lkfn-20220930.xsd#lkfn_NonWorkingCapitalLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_e001493c-a361-4d36-86f5-340b38eed650" xlink:to="loc_lkfn_NonWorkingCapitalLoansMember_9bacba89-c896-4f96-84dc-af16d949eb59" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialRealEstateMember_9d564330-7f6f-4ddd-adba-38252a3982fa" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommercialRealEstateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_e001493c-a361-4d36-86f5-340b38eed650" xlink:to="loc_us-gaap_CommercialRealEstateMember_9d564330-7f6f-4ddd-adba-38252a3982fa" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_OwnerOccupiedLoansMember_6072ef89-5e0f-4fa7-b66a-9162c31dbfb0" xlink:href="lkfn-20220930.xsd#lkfn_OwnerOccupiedLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_e001493c-a361-4d36-86f5-340b38eed650" xlink:to="loc_lkfn_OwnerOccupiedLoansMember_6072ef89-5e0f-4fa7-b66a-9162c31dbfb0" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_NonownerOccupiedLoansMember_a628ad70-7392-4382-a6e0-4ff04b083dfc" xlink:href="lkfn-20220930.xsd#lkfn_NonownerOccupiedLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_e001493c-a361-4d36-86f5-340b38eed650" xlink:to="loc_lkfn_NonownerOccupiedLoansMember_a628ad70-7392-4382-a6e0-4ff04b083dfc" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_MultifamilyLoansMember_2dd5afbe-1100-4f01-ad8c-916753df3543" xlink:href="lkfn-20220930.xsd#lkfn_MultifamilyLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_e001493c-a361-4d36-86f5-340b38eed650" xlink:to="loc_lkfn_MultifamilyLoansMember_2dd5afbe-1100-4f01-ad8c-916753df3543" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_LoansSecuredByFarmlandMember_5324dce7-5929-4b8e-930f-717a83980485" xlink:href="lkfn-20220930.xsd#lkfn_LoansSecuredByFarmlandMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_e001493c-a361-4d36-86f5-340b38eed650" xlink:to="loc_lkfn_LoansSecuredByFarmlandMember_5324dce7-5929-4b8e-930f-717a83980485" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_LoansForAgriculturalProductionMember_31606884-bb7c-431b-a588-bef848e03d30" xlink:href="lkfn-20220930.xsd#lkfn_LoansForAgriculturalProductionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_e001493c-a361-4d36-86f5-340b38eed650" xlink:to="loc_lkfn_LoansForAgriculturalProductionMember_31606884-bb7c-431b-a588-bef848e03d30" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_OtherCommercialLoansMember_c18c797d-1937-45f1-8e17-eef3d628cf43" xlink:href="lkfn-20220930.xsd#lkfn_OtherCommercialLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_e001493c-a361-4d36-86f5-340b38eed650" xlink:to="loc_lkfn_OtherCommercialLoansMember_c18c797d-1937-45f1-8e17-eef3d628cf43" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FirstMortgageMember_345c8e84-32a5-43e9-b436-715a99dc1856" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FirstMortgageMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_e001493c-a361-4d36-86f5-340b38eed650" xlink:to="loc_us-gaap_FirstMortgageMember_345c8e84-32a5-43e9-b436-715a99dc1856" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecondMortgageMember_25434ca1-7c4c-479f-b49c-7d989c72cb47" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SecondMortgageMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_e001493c-a361-4d36-86f5-340b38eed650" xlink:to="loc_us-gaap_SecondMortgageMember_25434ca1-7c4c-479f-b49c-7d989c72cb47" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResidentialMortgageMember_c1d1a1a9-5535-4fa2-b80f-ebf32dc19001" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ResidentialMortgageMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_e001493c-a361-4d36-86f5-340b38eed650" xlink:to="loc_us-gaap_ResidentialMortgageMember_c1d1a1a9-5535-4fa2-b80f-ebf32dc19001" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_OtherConsumerLoansMember_34631220-fb69-4f15-b7d6-e06cc1a7e894" xlink:href="lkfn-20220930.xsd#lkfn_OtherConsumerLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_e001493c-a361-4d36-86f5-340b38eed650" xlink:to="loc_lkfn_OtherConsumerLoansMember_34631220-fb69-4f15-b7d6-e06cc1a7e894" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablesPeriodPastDueAxis_8944490b-a36a-4aa2-92ef-0716a3142882" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivablesPeriodPastDueAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable_7fea3fb7-e5c6-478f-838f-0c76ac365901" xlink:to="loc_us-gaap_FinancingReceivablesPeriodPastDueAxis_8944490b-a36a-4aa2-92ef-0716a3142882" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablesPeriodPastDueDomain_8944490b-a36a-4aa2-92ef-0716a3142882_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivablesPeriodPastDueDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivablesPeriodPastDueAxis_8944490b-a36a-4aa2-92ef-0716a3142882" xlink:to="loc_us-gaap_FinancingReceivablesPeriodPastDueDomain_8944490b-a36a-4aa2-92ef-0716a3142882_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablesPeriodPastDueDomain_41aea6bf-fc9b-42aa-a722-2c4676fc501e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivablesPeriodPastDueDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivablesPeriodPastDueAxis_8944490b-a36a-4aa2-92ef-0716a3142882" xlink:to="loc_us-gaap_FinancingReceivablesPeriodPastDueDomain_41aea6bf-fc9b-42aa-a722-2c4676fc501e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialAssetNotPastDueMember_b21d58b9-1650-444b-909b-7a079679b27b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancialAssetNotPastDueMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablesPeriodPastDueDomain_41aea6bf-fc9b-42aa-a722-2c4676fc501e" xlink:to="loc_us-gaap_FinancialAssetNotPastDueMember_b21d58b9-1650-444b-909b-7a079679b27b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_FinancialAsset30To89DaysPastDueMember_99d1690b-f8c4-438f-b355-16597c7fd80e" xlink:href="lkfn-20220930.xsd#lkfn_FinancialAsset30To89DaysPastDueMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablesPeriodPastDueDomain_41aea6bf-fc9b-42aa-a722-2c4676fc501e" xlink:to="loc_lkfn_FinancialAsset30To89DaysPastDueMember_99d1690b-f8c4-438f-b355-16597c7fd80e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablesEqualToGreaterThan90DaysPastDueMember_1e5c1efc-cc2e-4e93-b0c2-67f486bcceb8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivablesEqualToGreaterThan90DaysPastDueMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablesPeriodPastDueDomain_41aea6bf-fc9b-42aa-a722-2c4676fc501e" xlink:to="loc_us-gaap_FinancingReceivablesEqualToGreaterThan90DaysPastDueMember_1e5c1efc-cc2e-4e93-b0c2-67f486bcceb8" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialAssetPastDueMember_e64f28ee-2ab1-47e7-b761-9b0674f5c306" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancialAssetPastDueMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablesPeriodPastDueDomain_41aea6bf-fc9b-42aa-a722-2c4676fc501e" xlink:to="loc_us-gaap_FinancialAssetPastDueMember_e64f28ee-2ab1-47e7-b761-9b0674f5c306" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.lakeland.com/role/ALLOWANCEFORCREDITLOSSESANDCREDITQUALITYAmortizedCostBasisOfCollateralDependentLoansDetails" xlink:type="simple" xlink:href="lkfn-20220930.xsd#ALLOWANCEFORCREDITLOSSESANDCREDITQUALITYAmortizedCostBasisOfCollateralDependentLoansDetails"/>
  <link:definitionLink xlink:role="http://www.lakeland.com/role/ALLOWANCEFORCREDITLOSSESANDCREDITQUALITYAmortizedCostBasisOfCollateralDependentLoansDetails" xlink:type="extended" id="i020f37815f8d449fa8660b850dfacfe1_ALLOWANCEFORCREDITLOSSESANDCREDITQUALITYAmortizedCostBasisOfCollateralDependentLoansDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_cf97a8f1-7bb9-4efa-b6dd-0f4f09f61533" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_FinancingReceivableRealEstateAmortizedCost_6a8fc96e-2194-4a9a-a169-89a7edcd9e5e" xlink:href="lkfn-20220930.xsd#lkfn_FinancingReceivableRealEstateAmortizedCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_cf97a8f1-7bb9-4efa-b6dd-0f4f09f61533" xlink:to="loc_lkfn_FinancingReceivableRealEstateAmortizedCost_6a8fc96e-2194-4a9a-a169-89a7edcd9e5e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_FinancingReceivableGeneralBusinessAssetsAmortizedCost_d9b82ee4-035e-495e-aa22-1c1bf12e1d09" xlink:href="lkfn-20220930.xsd#lkfn_FinancingReceivableGeneralBusinessAssetsAmortizedCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_cf97a8f1-7bb9-4efa-b6dd-0f4f09f61533" xlink:to="loc_lkfn_FinancingReceivableGeneralBusinessAssetsAmortizedCost_d9b82ee4-035e-495e-aa22-1c1bf12e1d09" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_FinancingReceivableAmortizedCostOther_39870cad-ecaa-4707-b2e3-4440c0013792" xlink:href="lkfn-20220930.xsd#lkfn_FinancingReceivableAmortizedCostOther"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_cf97a8f1-7bb9-4efa-b6dd-0f4f09f61533" xlink:to="loc_lkfn_FinancingReceivableAmortizedCostOther_39870cad-ecaa-4707-b2e3-4440c0013792" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_FinancingReceivableCollateralDependentBorrowingsAmortizedCost_9d65bd9e-f316-4e3a-bcc1-6e07c47f06e0" xlink:href="lkfn-20220930.xsd#lkfn_FinancingReceivableCollateralDependentBorrowingsAmortizedCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_cf97a8f1-7bb9-4efa-b6dd-0f4f09f61533" xlink:to="loc_lkfn_FinancingReceivableCollateralDependentBorrowingsAmortizedCost_9d65bd9e-f316-4e3a-bcc1-6e07c47f06e0" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable_75426ae8-1caf-4141-b384-c83efa5c74bb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_cf97a8f1-7bb9-4efa-b6dd-0f4f09f61533" xlink:to="loc_us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable_75426ae8-1caf-4141-b384-c83efa5c74bb" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_93afe128-21aa-4bb7-9842-423041c4d690" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable_75426ae8-1caf-4141-b384-c83efa5c74bb" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_93afe128-21aa-4bb7-9842-423041c4d690" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_93afe128-21aa-4bb7-9842-423041c4d690_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_93afe128-21aa-4bb7-9842-423041c4d690" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_93afe128-21aa-4bb7-9842-423041c4d690_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_e78917b6-b971-4446-a834-0e32e25f756f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_93afe128-21aa-4bb7-9842-423041c4d690" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_e78917b6-b971-4446-a834-0e32e25f756f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_CollateralDependentLoansMember_f8fc81f0-4da1-4a62-8cf8-9e92020a8baa" xlink:href="lkfn-20220930.xsd#lkfn_CollateralDependentLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_e78917b6-b971-4446-a834-0e32e25f756f" xlink:to="loc_lkfn_CollateralDependentLoansMember_f8fc81f0-4da1-4a62-8cf8-9e92020a8baa" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_TotalCommercialAndIndustrialLoansMember_6ea4d9f8-217f-4a09-8c32-bff926083066" xlink:href="lkfn-20220930.xsd#lkfn_TotalCommercialAndIndustrialLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_CollateralDependentLoansMember_f8fc81f0-4da1-4a62-8cf8-9e92020a8baa" xlink:to="loc_lkfn_TotalCommercialAndIndustrialLoansMember_6ea4d9f8-217f-4a09-8c32-bff926083066" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialRealEstatePortfolioSegmentMember_f847e199-071c-4f6b-93c8-670cdd70cd16" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommercialRealEstatePortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_CollateralDependentLoansMember_f8fc81f0-4da1-4a62-8cf8-9e92020a8baa" xlink:to="loc_us-gaap_CommercialRealEstatePortfolioSegmentMember_f847e199-071c-4f6b-93c8-670cdd70cd16" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_AgriBusinessAndAgriculturalLoansMember_51954b97-5555-43a4-8212-58675d4e19a1" xlink:href="lkfn-20220930.xsd#lkfn_AgriBusinessAndAgriculturalLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_CollateralDependentLoansMember_f8fc81f0-4da1-4a62-8cf8-9e92020a8baa" xlink:to="loc_lkfn_AgriBusinessAndAgriculturalLoansMember_51954b97-5555-43a4-8212-58675d4e19a1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResidentialPortfolioSegmentMember_bd16adef-4eda-41a4-a40b-e108df4d1895" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ResidentialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_CollateralDependentLoansMember_f8fc81f0-4da1-4a62-8cf8-9e92020a8baa" xlink:to="loc_us-gaap_ResidentialPortfolioSegmentMember_bd16adef-4eda-41a4-a40b-e108df4d1895" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_b105bf76-2aac-44af-8499-7009eb82dbf9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivablePortfolioSegmentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable_75426ae8-1caf-4141-b384-c83efa5c74bb" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_b105bf76-2aac-44af-8499-7009eb82dbf9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_b105bf76-2aac-44af-8499-7009eb82dbf9_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_b105bf76-2aac-44af-8499-7009eb82dbf9" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_b105bf76-2aac-44af-8499-7009eb82dbf9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_d07d3581-0407-418b-b409-482820c9cc23" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_b105bf76-2aac-44af-8499-7009eb82dbf9" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_d07d3581-0407-418b-b409-482820c9cc23" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditMember_63fdd2b6-0d7a-40fd-b94c-45d61272e5bb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LineOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_d07d3581-0407-418b-b409-482820c9cc23" xlink:to="loc_us-gaap_LineOfCreditMember_63fdd2b6-0d7a-40fd-b94c-45d61272e5bb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_NonWorkingCapitalLoansMember_943f50fa-3529-4904-99a6-0bc514c34429" xlink:href="lkfn-20220930.xsd#lkfn_NonWorkingCapitalLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_d07d3581-0407-418b-b409-482820c9cc23" xlink:to="loc_lkfn_NonWorkingCapitalLoansMember_943f50fa-3529-4904-99a6-0bc514c34429" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_OwnerOccupiedLoansMember_de08e561-cf7c-4677-875f-6cf59733d780" xlink:href="lkfn-20220930.xsd#lkfn_OwnerOccupiedLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_d07d3581-0407-418b-b409-482820c9cc23" xlink:to="loc_lkfn_OwnerOccupiedLoansMember_de08e561-cf7c-4677-875f-6cf59733d780" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_LoansSecuredByFarmlandMember_e52980dd-b736-40f6-9ee9-819ff79bb543" xlink:href="lkfn-20220930.xsd#lkfn_LoansSecuredByFarmlandMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_d07d3581-0407-418b-b409-482820c9cc23" xlink:to="loc_lkfn_LoansSecuredByFarmlandMember_e52980dd-b736-40f6-9ee9-819ff79bb543" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FirstMortgageMember_d9d21bdc-71f1-4875-8a69-e49c0810b4dd" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FirstMortgageMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_d07d3581-0407-418b-b409-482820c9cc23" xlink:to="loc_us-gaap_FirstMortgageMember_d9d21bdc-71f1-4875-8a69-e49c0810b4dd" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_JuniorLienMember_a68e58df-47d1-46b8-bfdc-32c23b78bf67" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_JuniorLienMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_d07d3581-0407-418b-b409-482820c9cc23" xlink:to="loc_us-gaap_JuniorLienMember_a68e58df-47d1-46b8-bfdc-32c23b78bf67" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_OtherConsumerLoansMember_c40947ca-b3af-4be4-9057-ab58866857d2" xlink:href="lkfn-20220930.xsd#lkfn_OtherConsumerLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_d07d3581-0407-418b-b409-482820c9cc23" xlink:to="loc_lkfn_OtherConsumerLoansMember_c40947ca-b3af-4be4-9057-ab58866857d2" xlink:type="arc" order="6"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.lakeland.com/role/BORROWINGSLongtermBorrowingsDetails" xlink:type="simple" xlink:href="lkfn-20220930.xsd#BORROWINGSLongtermBorrowingsDetails"/>
  <link:definitionLink xlink:role="http://www.lakeland.com/role/BORROWINGSLongtermBorrowingsDetails" xlink:type="extended" id="idab20a7517e44486bcb098d7b53f329f_BORROWINGSLongtermBorrowingsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_9f43eed5-c0b8-475e-88fd-ce31801146ef" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FederalHomeLoanBankAdvancesLongTerm_54a87f69-b342-4faf-b5bc-6263cf73503c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FederalHomeLoanBankAdvancesLongTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_9f43eed5-c0b8-475e-88fd-ce31801146ef" xlink:to="loc_us-gaap_FederalHomeLoanBankAdvancesLongTerm_54a87f69-b342-4faf-b5bc-6263cf73503c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTerm_97fb5d6e-ee86-4ba4-83b6-5d62d72fa29d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_9f43eed5-c0b8-475e-88fd-ce31801146ef" xlink:to="loc_us-gaap_DebtInstrumentTerm_97fb5d6e-ee86-4ba4-83b6-5d62d72fa29d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_70a82576-8e67-480b-9632-70fb4ca7294b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentInterestRateStatedPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_9f43eed5-c0b8-475e-88fd-ce31801146ef" xlink:to="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_70a82576-8e67-480b-9632-70fb4ca7294b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_d31d722a-2936-4a47-94e4-617361920dd0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_9f43eed5-c0b8-475e-88fd-ce31801146ef" xlink:to="loc_us-gaap_DebtInstrumentTable_d31d722a-2936-4a47-94e4-617361920dd0" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_59b27886-fc20-45b5-b80e-2ae2c91f8628" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_d31d722a-2936-4a47-94e4-617361920dd0" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_59b27886-fc20-45b5-b80e-2ae2c91f8628" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_59b27886-fc20-45b5-b80e-2ae2c91f8628_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_59b27886-fc20-45b5-b80e-2ae2c91f8628" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_59b27886-fc20-45b5-b80e-2ae2c91f8628_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_ff804c61-17b3-4b24-afd0-76fab5a19359" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_59b27886-fc20-45b5-b80e-2ae2c91f8628" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_ff804c61-17b3-4b24-afd0-76fab5a19359" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FederalHomeLoanBankAdvancesMember_5e3e8aed-1de3-414b-a1e1-956db737bcea" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FederalHomeLoanBankAdvancesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_ff804c61-17b3-4b24-afd0-76fab5a19359" xlink:to="loc_us-gaap_FederalHomeLoanBankAdvancesMember_5e3e8aed-1de3-414b-a1e1-956db737bcea" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_244d0fc6-feb4-4173-ae6a-2acfad7a2308" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_d31d722a-2936-4a47-94e4-617361920dd0" xlink:to="loc_us-gaap_DebtInstrumentAxis_244d0fc6-feb4-4173-ae6a-2acfad7a2308" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_244d0fc6-feb4-4173-ae6a-2acfad7a2308_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_244d0fc6-feb4-4173-ae6a-2acfad7a2308" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_244d0fc6-feb4-4173-ae6a-2acfad7a2308_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_5bf485d0-eb89-4948-8fcb-d4666cbfdda9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_244d0fc6-feb4-4173-ae6a-2acfad7a2308" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_5bf485d0-eb89-4948-8fcb-d4666cbfdda9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_FederalHomeLoanBankAdvanceTenYearPutableSixMonthBermudanFixedRateDueMarch42030Member_deac2f91-36f2-4772-b9bd-9fe670c266c2" xlink:href="lkfn-20220930.xsd#lkfn_FederalHomeLoanBankAdvanceTenYearPutableSixMonthBermudanFixedRateDueMarch42030Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_5bf485d0-eb89-4948-8fcb-d4666cbfdda9" xlink:to="loc_lkfn_FederalHomeLoanBankAdvanceTenYearPutableSixMonthBermudanFixedRateDueMarch42030Member_deac2f91-36f2-4772-b9bd-9fe670c266c2" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.lakeland.com/role/BORROWINGSRevolvingCreditAgreementDetails" xlink:type="simple" xlink:href="lkfn-20220930.xsd#BORROWINGSRevolvingCreditAgreementDetails"/>
  <link:definitionLink xlink:role="http://www.lakeland.com/role/BORROWINGSRevolvingCreditAgreementDetails" xlink:type="extended" id="idb0bfb26cd254f788d76459b82ae0888_BORROWINGSRevolvingCreditAgreementDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityLineItems_c9b90f4e-a790-49c8-8fb9-db45068209e9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LineOfCreditFacilityLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_45646c9f-218e-44ef-867d-c7beb1408a08" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_c9b90f4e-a790-49c8-8fb9-db45068209e9" xlink:to="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_45646c9f-218e-44ef-867d-c7beb1408a08" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTerm_12e0767b-76be-4905-b8fb-4fe6857cba00" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_c9b90f4e-a790-49c8-8fb9-db45068209e9" xlink:to="loc_us-gaap_DebtInstrumentTerm_12e0767b-76be-4905-b8fb-4fe6857cba00" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCredit_929533db-cdb6-466e-8fb2-1de575e492a7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LineOfCredit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_c9b90f4e-a790-49c8-8fb9-db45068209e9" xlink:to="loc_us-gaap_LineOfCredit_929533db-cdb6-466e-8fb2-1de575e492a7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityTable_e110eea8-3894-474e-ac95-01ce3edc81ba" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LineOfCreditFacilityTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_c9b90f4e-a790-49c8-8fb9-db45068209e9" xlink:to="loc_us-gaap_LineOfCreditFacilityTable_e110eea8-3894-474e-ac95-01ce3edc81ba" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityAxis_2c7a14e3-34df-469e-8ebe-b8595645b71d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_e110eea8-3894-474e-ac95-01ce3edc81ba" xlink:to="loc_us-gaap_CreditFacilityAxis_2c7a14e3-34df-469e-8ebe-b8595645b71d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_2c7a14e3-34df-469e-8ebe-b8595645b71d_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CreditFacilityAxis_2c7a14e3-34df-469e-8ebe-b8595645b71d" xlink:to="loc_us-gaap_CreditFacilityDomain_2c7a14e3-34df-469e-8ebe-b8595645b71d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_44d739d0-b993-44ef-bad7-c388b5e16c6d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CreditFacilityAxis_2c7a14e3-34df-469e-8ebe-b8595645b71d" xlink:to="loc_us-gaap_CreditFacilityDomain_44d739d0-b993-44ef-bad7-c388b5e16c6d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevolvingCreditFacilityMember_44528f38-ed69-43c0-902e-4549a73cc0b3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RevolvingCreditFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_44d739d0-b993-44ef-bad7-c388b5e16c6d" xlink:to="loc_us-gaap_RevolvingCreditFacilityMember_44528f38-ed69-43c0-902e-4549a73cc0b3" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.lakeland.com/role/FAIRVALUEDISCLOSURESAdditionalInformationDetails" xlink:type="simple" xlink:href="lkfn-20220930.xsd#FAIRVALUEDISCLOSURESAdditionalInformationDetails"/>
  <link:definitionLink xlink:role="http://www.lakeland.com/role/FAIRVALUEDISCLOSURESAdditionalInformationDetails" xlink:type="extended" id="iafce511382244c5aa510d5cad71a95c3_FAIRVALUEDISCLOSURESAdditionalInformationDetails">
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_AssetsPledgedAsCollateralForLoansReceivableLineItems_88df5233-5290-4183-8fea-7b72b9972f68" xlink:href="lkfn-20220930.xsd#lkfn_AssetsPledgedAsCollateralForLoansReceivableLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_PercentageOfVariance_10a0b57c-92ab-4d1f-8b14-f64e689d71a3" xlink:href="lkfn-20220930.xsd#lkfn_PercentageOfVariance"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_AssetsPledgedAsCollateralForLoansReceivableLineItems_88df5233-5290-4183-8fea-7b72b9972f68" xlink:to="loc_lkfn_PercentageOfVariance_10a0b57c-92ab-4d1f-8b14-f64e689d71a3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_AmountOfVariance_e75d7df6-0c2e-4f0a-bde8-543fda2ac9f1" xlink:href="lkfn-20220930.xsd#lkfn_AmountOfVariance"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_AssetsPledgedAsCollateralForLoansReceivableLineItems_88df5233-5290-4183-8fea-7b72b9972f68" xlink:to="loc_lkfn_AmountOfVariance_e75d7df6-0c2e-4f0a-bde8-543fda2ac9f1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_PercentageOfDiscountFromAppraisedValue_94e8539f-5b8a-4e15-a022-d3247789a339" xlink:href="lkfn-20220930.xsd#lkfn_PercentageOfDiscountFromAppraisedValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_AssetsPledgedAsCollateralForLoansReceivableLineItems_88df5233-5290-4183-8fea-7b72b9972f68" xlink:to="loc_lkfn_PercentageOfDiscountFromAppraisedValue_94e8539f-5b8a-4e15-a022-d3247789a339" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ServicingAssetAtFairValueAmount_a760f102-f360-477d-bad4-bb7fc46bc444" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ServicingAssetAtFairValueAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_AssetsPledgedAsCollateralForLoansReceivableLineItems_88df5233-5290-4183-8fea-7b72b9972f68" xlink:to="loc_us-gaap_ServicingAssetAtFairValueAmount_a760f102-f360-477d-bad4-bb7fc46bc444" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ServicingAssetAtAmortizedValue_efb418c1-45d3-4fef-87de-7f33e2acb69b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ServicingAssetAtAmortizedValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_AssetsPledgedAsCollateralForLoansReceivableLineItems_88df5233-5290-4183-8fea-7b72b9972f68" xlink:to="loc_us-gaap_ServicingAssetAtAmortizedValue_efb418c1-45d3-4fef-87de-7f33e2acb69b" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ServicingAssetAtAmortizedCostOtherThanTemporaryImpairments_dd0e8507-794a-4e3e-bca0-74fc8ef8888b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ServicingAssetAtAmortizedCostOtherThanTemporaryImpairments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_AssetsPledgedAsCollateralForLoansReceivableLineItems_88df5233-5290-4183-8fea-7b72b9972f68" xlink:to="loc_us-gaap_ServicingAssetAtAmortizedCostOtherThanTemporaryImpairments_dd0e8507-794a-4e3e-bca0-74fc8ef8888b" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_WeightedAverageMaturityOfResidentialMortgages_2ee9e75f-700c-43e3-81cf-1d90fdac7964" xlink:href="lkfn-20220930.xsd#lkfn_WeightedAverageMaturityOfResidentialMortgages"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_AssetsPledgedAsCollateralForLoansReceivableLineItems_88df5233-5290-4183-8fea-7b72b9972f68" xlink:to="loc_lkfn_WeightedAverageMaturityOfResidentialMortgages_2ee9e75f-700c-43e3-81cf-1d90fdac7964" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ServicingAssetsAndServicingLiabilitiesAtFairValueAssumptionsUsedToEstimateFairValueQualitativeInformation_41c12ae9-ec06-409f-a2f2-327e46cf5c35" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ServicingAssetsAndServicingLiabilitiesAtFairValueAssumptionsUsedToEstimateFairValueQualitativeInformation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_AssetsPledgedAsCollateralForLoansReceivableLineItems_88df5233-5290-4183-8fea-7b72b9972f68" xlink:to="loc_us-gaap_ServicingAssetsAndServicingLiabilitiesAtFairValueAssumptionsUsedToEstimateFairValueQualitativeInformation_41c12ae9-ec06-409f-a2f2-327e46cf5c35" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ServicingAssetsAndServicingLiabilitiesAtFairValueAssumptionsUsedToEstimateFairValueDiscountRate_e21e40a4-0801-4408-bbcc-57caa3706f13" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ServicingAssetsAndServicingLiabilitiesAtFairValueAssumptionsUsedToEstimateFairValueDiscountRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_AssetsPledgedAsCollateralForLoansReceivableLineItems_88df5233-5290-4183-8fea-7b72b9972f68" xlink:to="loc_us-gaap_ServicingAssetsAndServicingLiabilitiesAtFairValueAssumptionsUsedToEstimateFairValueDiscountRate_e21e40a4-0801-4408-bbcc-57caa3706f13" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_AssetsPledgedAsCollateralForLoansReceivableTable_cd597c6e-3627-430d-bb65-f85a7a165d4a" xlink:href="lkfn-20220930.xsd#lkfn_AssetsPledgedAsCollateralForLoansReceivableTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_lkfn_AssetsPledgedAsCollateralForLoansReceivableLineItems_88df5233-5290-4183-8fea-7b72b9972f68" xlink:to="loc_lkfn_AssetsPledgedAsCollateralForLoansReceivableTable_cd597c6e-3627-430d-bb65-f85a7a165d4a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentTypeAxis_d3753337-6467-4c7f-8781-b7d978cce359" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InvestmentTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lkfn_AssetsPledgedAsCollateralForLoansReceivableTable_cd597c6e-3627-430d-bb65-f85a7a165d4a" xlink:to="loc_us-gaap_InvestmentTypeAxis_d3753337-6467-4c7f-8781-b7d978cce359" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentTypeCategorizationMember_d3753337-6467-4c7f-8781-b7d978cce359_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InvestmentTypeCategorizationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_InvestmentTypeAxis_d3753337-6467-4c7f-8781-b7d978cce359" xlink:to="loc_us-gaap_InvestmentTypeCategorizationMember_d3753337-6467-4c7f-8781-b7d978cce359_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentTypeCategorizationMember_c0536603-14fb-4418-9d82-1ead15e7cd1a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InvestmentTypeCategorizationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_InvestmentTypeAxis_d3753337-6467-4c7f-8781-b7d978cce359" xlink:to="loc_us-gaap_InvestmentTypeCategorizationMember_c0536603-14fb-4418-9d82-1ead15e7cd1a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MunicipalNotesMember_5f3fc0df-b8c6-498d-a78f-d777c7c44fc8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_MunicipalNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InvestmentTypeCategorizationMember_c0536603-14fb-4418-9d82-1ead15e7cd1a" xlink:to="loc_us-gaap_MunicipalNotesMember_5f3fc0df-b8c6-498d-a78f-d777c7c44fc8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USGovernmentAgenciesShorttermDebtSecuritiesMember_4b09bf90-fbb8-4db7-8b6a-94156333c639" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_USGovernmentAgenciesShorttermDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InvestmentTypeCategorizationMember_c0536603-14fb-4418-9d82-1ead15e7cd1a" xlink:to="loc_us-gaap_USGovernmentAgenciesShorttermDebtSecuritiesMember_4b09bf90-fbb8-4db7-8b6a-94156333c639" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USTreasurySecuritiesMember_84214549-f171-41fb-8567-a121977856b7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_USTreasurySecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InvestmentTypeCategorizationMember_c0536603-14fb-4418-9d82-1ead15e7cd1a" xlink:to="loc_us-gaap_USTreasurySecuritiesMember_84214549-f171-41fb-8567-a121977856b7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_ce5096ca-d693-4cff-8d46-0b1f8a785fe9" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lkfn_AssetsPledgedAsCollateralForLoansReceivableTable_cd597c6e-3627-430d-bb65-f85a7a165d4a" xlink:to="loc_srt_RangeAxis_ce5096ca-d693-4cff-8d46-0b1f8a785fe9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_ce5096ca-d693-4cff-8d46-0b1f8a785fe9_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_ce5096ca-d693-4cff-8d46-0b1f8a785fe9" xlink:to="loc_srt_RangeMember_ce5096ca-d693-4cff-8d46-0b1f8a785fe9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_55544d1f-5919-4af0-a531-f01e019053c8" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_ce5096ca-d693-4cff-8d46-0b1f8a785fe9" xlink:to="loc_srt_RangeMember_55544d1f-5919-4af0-a531-f01e019053c8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_16bf935d-44e5-44ee-a885-6132770841a8" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_55544d1f-5919-4af0-a531-f01e019053c8" xlink:to="loc_srt_MinimumMember_16bf935d-44e5-44ee-a885-6132770841a8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_c6ee0e68-aff6-43f2-b23c-0c3505d0e557" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_55544d1f-5919-4af0-a531-f01e019053c8" xlink:to="loc_srt_MaximumMember_c6ee0e68-aff6-43f2-b23c-0c3505d0e557" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PledgedStatusAxis_41583286-d82e-48b2-a05d-1e6b74484c11" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PledgedStatusAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lkfn_AssetsPledgedAsCollateralForLoansReceivableTable_cd597c6e-3627-430d-bb65-f85a7a165d4a" xlink:to="loc_us-gaap_PledgedStatusAxis_41583286-d82e-48b2-a05d-1e6b74484c11" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PledgedStatusDomain_41583286-d82e-48b2-a05d-1e6b74484c11_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PledgedStatusDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PledgedStatusAxis_41583286-d82e-48b2-a05d-1e6b74484c11" xlink:to="loc_us-gaap_PledgedStatusDomain_41583286-d82e-48b2-a05d-1e6b74484c11_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PledgedStatusDomain_cc36290b-9c2e-41ee-9063-cbbb1d42269a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PledgedStatusDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PledgedStatusAxis_41583286-d82e-48b2-a05d-1e6b74484c11" xlink:to="loc_us-gaap_PledgedStatusDomain_cc36290b-9c2e-41ee-9063-cbbb1d42269a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_CommercialRealEstatesMember_7b39ebc0-39db-4185-8584-179b68abfcd4" xlink:href="lkfn-20220930.xsd#lkfn_CommercialRealEstatesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PledgedStatusDomain_cc36290b-9c2e-41ee-9063-cbbb1d42269a" xlink:to="loc_lkfn_CommercialRealEstatesMember_7b39ebc0-39db-4185-8584-179b68abfcd4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_InventoryFinishedGoodsMember_b94795d3-2d13-476c-b576-6063fc3d2644" xlink:href="lkfn-20220930.xsd#lkfn_InventoryFinishedGoodsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PledgedStatusDomain_cc36290b-9c2e-41ee-9063-cbbb1d42269a" xlink:to="loc_lkfn_InventoryFinishedGoodsMember_b94795d3-2d13-476c-b576-6063fc3d2644" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_FinishedGoodsMember_d5d1767b-1980-4712-b3a9-c41d3f83a269" xlink:href="lkfn-20220930.xsd#lkfn_FinishedGoodsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PledgedStatusDomain_cc36290b-9c2e-41ee-9063-cbbb1d42269a" xlink:to="loc_lkfn_FinishedGoodsMember_d5d1767b-1980-4712-b3a9-c41d3f83a269" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_InventoryWorkInProcessMember_1739af75-d45a-478e-9fab-ffd22ca97f85" xlink:href="lkfn-20220930.xsd#lkfn_InventoryWorkInProcessMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PledgedStatusDomain_cc36290b-9c2e-41ee-9063-cbbb1d42269a" xlink:to="loc_lkfn_InventoryWorkInProcessMember_1739af75-d45a-478e-9fab-ffd22ca97f85" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquipmentMember_721c13a8-7620-4ec4-9c83-f48407b9b395" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PledgedStatusDomain_cc36290b-9c2e-41ee-9063-cbbb1d42269a" xlink:to="loc_us-gaap_EquipmentMember_721c13a8-7620-4ec4-9c83-f48407b9b395" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_MarketableSecuritiesMember_7e79bb57-ec52-4fae-aa1b-0b0b178a65cd" xlink:href="lkfn-20220930.xsd#lkfn_MarketableSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PledgedStatusDomain_cc36290b-9c2e-41ee-9063-cbbb1d42269a" xlink:to="loc_lkfn_MarketableSecuritiesMember_7e79bb57-ec52-4fae-aa1b-0b0b178a65cd" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_326fad61-bb8d-4b58-bafb-1901f5f3c999" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lkfn_AssetsPledgedAsCollateralForLoansReceivableTable_cd597c6e-3627-430d-bb65-f85a7a165d4a" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_326fad61-bb8d-4b58-bafb-1901f5f3c999" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_326fad61-bb8d-4b58-bafb-1901f5f3c999_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_326fad61-bb8d-4b58-bafb-1901f5f3c999" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_326fad61-bb8d-4b58-bafb-1901f5f3c999_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_24e548b3-c413-4afe-89e3-0c6f80ee9f5e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_326fad61-bb8d-4b58-bafb-1901f5f3c999" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_24e548b3-c413-4afe-89e3-0c6f80ee9f5e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_aa79885f-5c92-43ce-b540-b3585caae258" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueInputsLevel3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_24e548b3-c413-4afe-89e3-0c6f80ee9f5e" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_aa79885f-5c92-43ce-b540-b3585caae258" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.lakeland.com/role/FAIRVALUEDISCLOSURESAssetsandLiabilitiesMeasuredAtFairValueonaRecurringBasisDetails" xlink:type="simple" xlink:href="lkfn-20220930.xsd#FAIRVALUEDISCLOSURESAssetsandLiabilitiesMeasuredAtFairValueonaRecurringBasisDetails"/>
  <link:definitionLink xlink:role="http://www.lakeland.com/role/FAIRVALUEDISCLOSURESAssetsandLiabilitiesMeasuredAtFairValueonaRecurringBasisDetails" xlink:type="extended" id="ia714cca540ef430482d45425daefc7f7_FAIRVALUEDISCLOSURESAssetsandLiabilitiesMeasuredAtFairValueonaRecurringBasisDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_d1c71f4d-9cfd-44a2-8c06-c4a2ec45ba15" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_AssetsMeasuredAtFairValueOnRecurringBasisAbstract_80c926ca-6f79-4874-a181-3f67d930b2c8" xlink:href="lkfn-20220930.xsd#lkfn_AssetsMeasuredAtFairValueOnRecurringBasisAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_d1c71f4d-9cfd-44a2-8c06-c4a2ec45ba15" xlink:to="loc_lkfn_AssetsMeasuredAtFairValueOnRecurringBasisAbstract_80c926ca-6f79-4874-a181-3f67d930b2c8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsFairValueDisclosureAbstract_ed2e5697-e2c9-405d-9cdc-174c03750a34" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AssetsFairValueDisclosureAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_AssetsMeasuredAtFairValueOnRecurringBasisAbstract_80c926ca-6f79-4874-a181-3f67d930b2c8" xlink:to="loc_us-gaap_AssetsFairValueDisclosureAbstract_ed2e5697-e2c9-405d-9cdc-174c03750a34" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_UsTreasurySecuritiesFairValueDisclosure_0107de28-b1eb-4199-8ebd-af7d70336cab" xlink:href="lkfn-20220930.xsd#lkfn_UsTreasurySecuritiesFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsFairValueDisclosureAbstract_ed2e5697-e2c9-405d-9cdc-174c03750a34" xlink:to="loc_lkfn_UsTreasurySecuritiesFairValueDisclosure_0107de28-b1eb-4199-8ebd-af7d70336cab" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_UsGovernmentAgenciesSecuritiesFairValueDisclosure_976d71de-8de9-4788-9008-2c67520c73d6" xlink:href="lkfn-20220930.xsd#lkfn_UsGovernmentAgenciesSecuritiesFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsFairValueDisclosureAbstract_ed2e5697-e2c9-405d-9cdc-174c03750a34" xlink:to="loc_lkfn_UsGovernmentAgenciesSecuritiesFairValueDisclosure_976d71de-8de9-4788-9008-2c67520c73d6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_MortgageBackedSecuritiesResidentialAvailableForSaleFairValueDisclosure_594ff3b7-1daf-449a-bc31-66ec88f5379e" xlink:href="lkfn-20220930.xsd#lkfn_MortgageBackedSecuritiesResidentialAvailableForSaleFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsFairValueDisclosureAbstract_ed2e5697-e2c9-405d-9cdc-174c03750a34" xlink:to="loc_lkfn_MortgageBackedSecuritiesResidentialAvailableForSaleFairValueDisclosure_594ff3b7-1daf-449a-bc31-66ec88f5379e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_MortgageBackedSecuritiesCommercialAvailableForSaleFairValueDisclosure_9fdb50b5-3c92-4459-bf97-8dcc4ea45ae8" xlink:href="lkfn-20220930.xsd#lkfn_MortgageBackedSecuritiesCommercialAvailableForSaleFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsFairValueDisclosureAbstract_ed2e5697-e2c9-405d-9cdc-174c03750a34" xlink:to="loc_lkfn_MortgageBackedSecuritiesCommercialAvailableForSaleFairValueDisclosure_9fdb50b5-3c92-4459-bf97-8dcc4ea45ae8" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_StateAndMunicipalSecuritiesFairValueDisclosure_8784df77-547e-453c-9630-333326b070b4" xlink:href="lkfn-20220930.xsd#lkfn_StateAndMunicipalSecuritiesFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsFairValueDisclosureAbstract_ed2e5697-e2c9-405d-9cdc-174c03750a34" xlink:to="loc_lkfn_StateAndMunicipalSecuritiesFairValueDisclosure_8784df77-547e-453c-9630-333326b070b4" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleExcludingAccruedInterest_4d8719d8-25a0-43fc-842c-7ab111ea9a90" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtSecuritiesAvailableForSaleExcludingAccruedInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsFairValueDisclosureAbstract_ed2e5697-e2c9-405d-9cdc-174c03750a34" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleExcludingAccruedInterest_4d8719d8-25a0-43fc-842c-7ab111ea9a90" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeAssets_34ab9057-9166-4513-88af-17a7eb73f86b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DerivativeAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsFairValueDisclosureAbstract_ed2e5697-e2c9-405d-9cdc-174c03750a34" xlink:to="loc_us-gaap_DerivativeAssets_34ab9057-9166-4513-88af-17a7eb73f86b" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestRateDerivativeAssetsAtFairValue_cc2d8c70-6285-418a-a154-c953cb24e5f4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InterestRateDerivativeAssetsAtFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsFairValueDisclosureAbstract_ed2e5697-e2c9-405d-9cdc-174c03750a34" xlink:to="loc_us-gaap_InterestRateDerivativeAssetsAtFairValue_cc2d8c70-6285-418a-a154-c953cb24e5f4" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsFairValueDisclosure_6a28c70b-9bcb-4651-9d07-458085712199" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AssetsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsFairValueDisclosureAbstract_ed2e5697-e2c9-405d-9cdc-174c03750a34" xlink:to="loc_us-gaap_AssetsFairValueDisclosure_6a28c70b-9bcb-4651-9d07-458085712199" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesFairValueDisclosureAbstract_5dd146d5-707b-47b8-9cb5-27bc647d4415" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LiabilitiesFairValueDisclosureAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_AssetsMeasuredAtFairValueOnRecurringBasisAbstract_80c926ca-6f79-4874-a181-3f67d930b2c8" xlink:to="loc_us-gaap_LiabilitiesFairValueDisclosureAbstract_5dd146d5-707b-47b8-9cb5-27bc647d4415" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeLiabilities_9f0d3821-48ac-4d36-8af8-f2a6f2145da8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DerivativeLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesFairValueDisclosureAbstract_5dd146d5-707b-47b8-9cb5-27bc647d4415" xlink:to="loc_us-gaap_DerivativeLiabilities_9f0d3821-48ac-4d36-8af8-f2a6f2145da8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestRateDerivativeLiabilitiesAtFairValue_e36f7c91-6817-42c3-89fc-013dd52231c7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InterestRateDerivativeLiabilitiesAtFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesFairValueDisclosureAbstract_5dd146d5-707b-47b8-9cb5-27bc647d4415" xlink:to="loc_us-gaap_InterestRateDerivativeLiabilitiesAtFairValue_e36f7c91-6817-42c3-89fc-013dd52231c7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesFairValueDisclosure_9b20a099-98bf-4c02-b842-b85606beebe5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LiabilitiesFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesFairValueDisclosureAbstract_5dd146d5-707b-47b8-9cb5-27bc647d4415" xlink:to="loc_us-gaap_LiabilitiesFairValueDisclosure_9b20a099-98bf-4c02-b842-b85606beebe5" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_dbb77ee0-5923-4794-9b6a-cbefb02c1e39" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_d1c71f4d-9cfd-44a2-8c06-c4a2ec45ba15" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_dbb77ee0-5923-4794-9b6a-cbefb02c1e39" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_ca66efd8-867c-4927-b7f1-13f6e38c476a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_dbb77ee0-5923-4794-9b6a-cbefb02c1e39" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_ca66efd8-867c-4927-b7f1-13f6e38c476a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_ca66efd8-867c-4927-b7f1-13f6e38c476a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_ca66efd8-867c-4927-b7f1-13f6e38c476a" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_ca66efd8-867c-4927-b7f1-13f6e38c476a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_2dd98fb6-9b5a-40e9-8d65-90c0a751a96c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_ca66efd8-867c-4927-b7f1-13f6e38c476a" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_2dd98fb6-9b5a-40e9-8d65-90c0a751a96c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_7cb5da49-11d1-4a20-bb35-d40ae79a8a76" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_2dd98fb6-9b5a-40e9-8d65-90c0a751a96c" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_7cb5da49-11d1-4a20-bb35-d40ae79a8a76" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_ece6bb03-4a58-4cda-a1c7-9472ca9904ff" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_2dd98fb6-9b5a-40e9-8d65-90c0a751a96c" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_ece6bb03-4a58-4cda-a1c7-9472ca9904ff" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_46c8251b-9994-426b-bf81-7a4e737c5eef" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueInputsLevel3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_2dd98fb6-9b5a-40e9-8d65-90c0a751a96c" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_46c8251b-9994-426b-bf81-7a4e737c5eef" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementFrequencyAxis_5b4cbd8b-18c3-4881-8024-44db1bd04a34" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueByMeasurementFrequencyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_dbb77ee0-5923-4794-9b6a-cbefb02c1e39" xlink:to="loc_us-gaap_FairValueByMeasurementFrequencyAxis_5b4cbd8b-18c3-4881-8024-44db1bd04a34" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_5b4cbd8b-18c3-4881-8024-44db1bd04a34_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_5b4cbd8b-18c3-4881-8024-44db1bd04a34" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_5b4cbd8b-18c3-4881-8024-44db1bd04a34_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_75a37d82-874b-450c-93cf-4710b84c6c92" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_5b4cbd8b-18c3-4881-8024-44db1bd04a34" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_75a37d82-874b-450c-93cf-4710b84c6c92" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsRecurringMember_14a1c3cb-7155-45d4-8302-1a19a32377e6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueMeasurementsRecurringMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementFrequencyDomain_75a37d82-874b-450c-93cf-4710b84c6c92" xlink:to="loc_us-gaap_FairValueMeasurementsRecurringMember_14a1c3cb-7155-45d4-8302-1a19a32377e6" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.lakeland.com/role/FAIRVALUEDISCLOSURESAssetsMeasuredatFairValueonaNonrecurringBasisDetails" xlink:type="simple" xlink:href="lkfn-20220930.xsd#FAIRVALUEDISCLOSURESAssetsMeasuredatFairValueonaNonrecurringBasisDetails"/>
  <link:definitionLink xlink:role="http://www.lakeland.com/role/FAIRVALUEDISCLOSURESAssetsMeasuredatFairValueonaNonrecurringBasisDetails" xlink:type="extended" id="i6f5bada008d244e3a073be60315286bc_FAIRVALUEDISCLOSURESAssetsMeasuredatFairValueonaNonrecurringBasisDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_4c640a10-a263-42dc-8f46-6eebf381c760" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_ImpairedLoansFairValueDisclosure_0b53ec5d-032e-49ae-b425-271d5a872ba3" xlink:href="lkfn-20220930.xsd#lkfn_ImpairedLoansFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_4c640a10-a263-42dc-8f46-6eebf381c760" xlink:to="loc_lkfn_ImpairedLoansFairValueDisclosure_0b53ec5d-032e-49ae-b425-271d5a872ba3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_OtherRealEstateOwnedFairValueDisclosure_1b3396ae-e104-4152-b721-baf326529ca1" xlink:href="lkfn-20220930.xsd#lkfn_OtherRealEstateOwnedFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_4c640a10-a263-42dc-8f46-6eebf381c760" xlink:to="loc_lkfn_OtherRealEstateOwnedFairValueDisclosure_1b3396ae-e104-4152-b721-baf326529ca1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsFairValueDisclosure_1e95fb53-551c-4089-aa25-e81883c8b675" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AssetsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_4c640a10-a263-42dc-8f46-6eebf381c760" xlink:to="loc_us-gaap_AssetsFairValueDisclosure_1e95fb53-551c-4089-aa25-e81883c8b675" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_224d3d30-b743-4195-a0b3-1545363ee0e6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_4c640a10-a263-42dc-8f46-6eebf381c760" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_224d3d30-b743-4195-a0b3-1545363ee0e6" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_dffdea90-00cf-441f-bc8b-5780daaefce5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_224d3d30-b743-4195-a0b3-1545363ee0e6" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_dffdea90-00cf-441f-bc8b-5780daaefce5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_dffdea90-00cf-441f-bc8b-5780daaefce5_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_dffdea90-00cf-441f-bc8b-5780daaefce5" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_dffdea90-00cf-441f-bc8b-5780daaefce5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_8531c605-4bd9-4d8c-a646-0f723ae521e2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_dffdea90-00cf-441f-bc8b-5780daaefce5" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_8531c605-4bd9-4d8c-a646-0f723ae521e2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_TotalCommercialAndIndustrialLoansMember_60788645-a807-4661-bcaf-f50bc0dd1506" xlink:href="lkfn-20220930.xsd#lkfn_TotalCommercialAndIndustrialLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_8531c605-4bd9-4d8c-a646-0f723ae521e2" xlink:to="loc_lkfn_TotalCommercialAndIndustrialLoansMember_60788645-a807-4661-bcaf-f50bc0dd1506" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialRealEstatePortfolioSegmentMember_82064f00-4cba-4338-a2cb-37961fee2e33" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommercialRealEstatePortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_8531c605-4bd9-4d8c-a646-0f723ae521e2" xlink:to="loc_us-gaap_CommercialRealEstatePortfolioSegmentMember_82064f00-4cba-4338-a2cb-37961fee2e33" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_AgribusinessAndAgriculturalMember_70dc900d-dd12-404a-98ac-aae0fb602537" xlink:href="lkfn-20220930.xsd#lkfn_AgribusinessAndAgriculturalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_8531c605-4bd9-4d8c-a646-0f723ae521e2" xlink:to="loc_lkfn_AgribusinessAndAgriculturalMember_70dc900d-dd12-404a-98ac-aae0fb602537" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_4f794e40-d718-4a98-95c5-2016c7f0b906" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_224d3d30-b743-4195-a0b3-1545363ee0e6" xlink:to="loc_us-gaap_FinancialInstrumentAxis_4f794e40-d718-4a98-95c5-2016c7f0b906" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_4f794e40-d718-4a98-95c5-2016c7f0b906_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_4f794e40-d718-4a98-95c5-2016c7f0b906" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_4f794e40-d718-4a98-95c5-2016c7f0b906_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_ab97aef5-7e08-420b-9bc2-d14a716b86e6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_4f794e40-d718-4a98-95c5-2016c7f0b906" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_ab97aef5-7e08-420b-9bc2-d14a716b86e6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditMember_b80bb2fd-6e7a-4e4f-a1b6-30dfba6f8983" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LineOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_ab97aef5-7e08-420b-9bc2-d14a716b86e6" xlink:to="loc_us-gaap_LineOfCreditMember_b80bb2fd-6e7a-4e4f-a1b6-30dfba6f8983" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_NonWorkingCapitalLoansMember_5e822272-eafc-4a56-9efd-ca4271b97a4a" xlink:href="lkfn-20220930.xsd#lkfn_NonWorkingCapitalLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_ab97aef5-7e08-420b-9bc2-d14a716b86e6" xlink:to="loc_lkfn_NonWorkingCapitalLoansMember_5e822272-eafc-4a56-9efd-ca4271b97a4a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_OwnerOccupiedLoansMember_8b8baad5-b5ba-400c-895d-247d8dcc6df7" xlink:href="lkfn-20220930.xsd#lkfn_OwnerOccupiedLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_ab97aef5-7e08-420b-9bc2-d14a716b86e6" xlink:to="loc_lkfn_OwnerOccupiedLoansMember_8b8baad5-b5ba-400c-895d-247d8dcc6df7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_LoansSecuredByFarmlandMember_775f8f5c-778f-4b5c-afae-4746531cdd6e" xlink:href="lkfn-20220930.xsd#lkfn_LoansSecuredByFarmlandMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_ab97aef5-7e08-420b-9bc2-d14a716b86e6" xlink:to="loc_lkfn_LoansSecuredByFarmlandMember_775f8f5c-778f-4b5c-afae-4746531cdd6e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_df2c2b60-3f85-44b3-afca-2535673c52b0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_224d3d30-b743-4195-a0b3-1545363ee0e6" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_df2c2b60-3f85-44b3-afca-2535673c52b0" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_df2c2b60-3f85-44b3-afca-2535673c52b0_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_df2c2b60-3f85-44b3-afca-2535673c52b0" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_df2c2b60-3f85-44b3-afca-2535673c52b0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_2c586205-eb01-4a89-80a4-2c302d7baef4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_df2c2b60-3f85-44b3-afca-2535673c52b0" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_2c586205-eb01-4a89-80a4-2c302d7baef4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_abff197f-45cf-4411-8e42-9fd09ce47504" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_2c586205-eb01-4a89-80a4-2c302d7baef4" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_abff197f-45cf-4411-8e42-9fd09ce47504" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_14742f7e-8ba5-4c24-9864-4f80bf6e99ea" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_2c586205-eb01-4a89-80a4-2c302d7baef4" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_14742f7e-8ba5-4c24-9864-4f80bf6e99ea" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_0b67cbfa-f57b-4db9-b6b3-da50b917aea5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueInputsLevel3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_2c586205-eb01-4a89-80a4-2c302d7baef4" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_0b67cbfa-f57b-4db9-b6b3-da50b917aea5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementFrequencyAxis_dedaa5f9-761a-46ce-a570-c57d71f591da" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueByMeasurementFrequencyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_224d3d30-b743-4195-a0b3-1545363ee0e6" xlink:to="loc_us-gaap_FairValueByMeasurementFrequencyAxis_dedaa5f9-761a-46ce-a570-c57d71f591da" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_dedaa5f9-761a-46ce-a570-c57d71f591da_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_dedaa5f9-761a-46ce-a570-c57d71f591da" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_dedaa5f9-761a-46ce-a570-c57d71f591da_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_f84b2c2a-29dd-4932-9e80-ac107cf0963f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_dedaa5f9-761a-46ce-a570-c57d71f591da" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_f84b2c2a-29dd-4932-9e80-ac107cf0963f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsNonrecurringMember_677106d6-ab35-4f16-b452-c0bd5194096b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueMeasurementsNonrecurringMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementFrequencyDomain_f84b2c2a-29dd-4932-9e80-ac107cf0963f" xlink:to="loc_us-gaap_FairValueMeasurementsNonrecurringMember_677106d6-ab35-4f16-b452-c0bd5194096b" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.lakeland.com/role/FAIRVALUEDISCLOSURESValuationMethodologyandUnobservableInputsforLevel3AssetsDetails" xlink:type="simple" xlink:href="lkfn-20220930.xsd#FAIRVALUEDISCLOSURESValuationMethodologyandUnobservableInputsforLevel3AssetsDetails"/>
  <link:definitionLink xlink:role="http://www.lakeland.com/role/FAIRVALUEDISCLOSURESValuationMethodologyandUnobservableInputsforLevel3AssetsDetails" xlink:type="extended" id="i357edaf4309549db977f07981a9c2847_FAIRVALUEDISCLOSURESValuationMethodologyandUnobservableInputsforLevel3AssetsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_ValuationMethodologyAndUnobservableInputsForLevel3AssetsMeasuredAtFairValueLineItems_dac956bf-0c29-42b8-b542-e578dfb7eeee" xlink:href="lkfn-20220930.xsd#lkfn_ValuationMethodologyAndUnobservableInputsForLevel3AssetsMeasuredAtFairValueLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_ImpairedLoansFairValueDisclosure_29b2578b-21b2-4acf-987a-e289ecbcc522" xlink:href="lkfn-20220930.xsd#lkfn_ImpairedLoansFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_ValuationMethodologyAndUnobservableInputsForLevel3AssetsMeasuredAtFairValueLineItems_dac956bf-0c29-42b8-b542-e578dfb7eeee" xlink:to="loc_lkfn_ImpairedLoansFairValueDisclosure_29b2578b-21b2-4acf-987a-e289ecbcc522" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentMeasurementInput_bd86eb8a-1a6d-4c40-a71b-835386004869" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtInstrumentMeasurementInput"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_ValuationMethodologyAndUnobservableInputsForLevel3AssetsMeasuredAtFairValueLineItems_dac956bf-0c29-42b8-b542-e578dfb7eeee" xlink:to="loc_us-gaap_DebtInstrumentMeasurementInput_bd86eb8a-1a6d-4c40-a71b-835386004869" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_ValuationMethodologyAndUnobservableInputsForLevel3AssetsMeasuredAtFairValueTable_c8e35a22-0006-4aa5-b651-19021408132e" xlink:href="lkfn-20220930.xsd#lkfn_ValuationMethodologyAndUnobservableInputsForLevel3AssetsMeasuredAtFairValueTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_lkfn_ValuationMethodologyAndUnobservableInputsForLevel3AssetsMeasuredAtFairValueLineItems_dac956bf-0c29-42b8-b542-e578dfb7eeee" xlink:to="loc_lkfn_ValuationMethodologyAndUnobservableInputsForLevel3AssetsMeasuredAtFairValueTable_c8e35a22-0006-4aa5-b651-19021408132e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_04e97c18-ac08-4299-ac59-c3cbfc711b46" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lkfn_ValuationMethodologyAndUnobservableInputsForLevel3AssetsMeasuredAtFairValueTable_c8e35a22-0006-4aa5-b651-19021408132e" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_04e97c18-ac08-4299-ac59-c3cbfc711b46" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_04e97c18-ac08-4299-ac59-c3cbfc711b46_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_04e97c18-ac08-4299-ac59-c3cbfc711b46" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_04e97c18-ac08-4299-ac59-c3cbfc711b46_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_77fd1160-b4a8-4113-82d2-4b52065c4ced" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_04e97c18-ac08-4299-ac59-c3cbfc711b46" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_77fd1160-b4a8-4113-82d2-4b52065c4ced" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_TotalCommercialAndIndustrialLoansMember_eabf9539-e3ae-4bf5-aef0-2e4a7c13054c" xlink:href="lkfn-20220930.xsd#lkfn_TotalCommercialAndIndustrialLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_77fd1160-b4a8-4113-82d2-4b52065c4ced" xlink:to="loc_lkfn_TotalCommercialAndIndustrialLoansMember_eabf9539-e3ae-4bf5-aef0-2e4a7c13054c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialRealEstatePortfolioSegmentMember_f17176b8-7dd5-42dc-9244-86f6b16140ec" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommercialRealEstatePortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_77fd1160-b4a8-4113-82d2-4b52065c4ced" xlink:to="loc_us-gaap_CommercialRealEstatePortfolioSegmentMember_f17176b8-7dd5-42dc-9244-86f6b16140ec" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_AgribusinessAndAgriculturalMember_2bd1bdaa-69f7-4562-836a-dcbe273017f9" xlink:href="lkfn-20220930.xsd#lkfn_AgribusinessAndAgriculturalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_77fd1160-b4a8-4113-82d2-4b52065c4ced" xlink:to="loc_lkfn_AgribusinessAndAgriculturalMember_2bd1bdaa-69f7-4562-836a-dcbe273017f9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_OtherRealEstateOwnedMember_eb76226c-3148-42c0-8a3f-cea3c5c84de6" xlink:href="lkfn-20220930.xsd#lkfn_OtherRealEstateOwnedMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_77fd1160-b4a8-4113-82d2-4b52065c4ced" xlink:to="loc_lkfn_OtherRealEstateOwnedMember_eb76226c-3148-42c0-8a3f-cea3c5c84de6" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementFrequencyAxis_f6062c81-5dd7-4da7-a3be-648456aab79c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueByMeasurementFrequencyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lkfn_ValuationMethodologyAndUnobservableInputsForLevel3AssetsMeasuredAtFairValueTable_c8e35a22-0006-4aa5-b651-19021408132e" xlink:to="loc_us-gaap_FairValueByMeasurementFrequencyAxis_f6062c81-5dd7-4da7-a3be-648456aab79c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_f6062c81-5dd7-4da7-a3be-648456aab79c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_f6062c81-5dd7-4da7-a3be-648456aab79c" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_f6062c81-5dd7-4da7-a3be-648456aab79c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_48d243fe-31ef-46dc-98e7-a2a6d810ba5a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_f6062c81-5dd7-4da7-a3be-648456aab79c" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_48d243fe-31ef-46dc-98e7-a2a6d810ba5a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsNonrecurringMember_c91e1859-c0ae-430e-bd75-2c453cc79c95" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueMeasurementsNonrecurringMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementFrequencyDomain_48d243fe-31ef-46dc-98e7-a2a6d810ba5a" xlink:to="loc_us-gaap_FairValueMeasurementsNonrecurringMember_c91e1859-c0ae-430e-bd75-2c453cc79c95" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_a0413c06-abd9-47f1-9c9a-14f65b345aa6" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lkfn_ValuationMethodologyAndUnobservableInputsForLevel3AssetsMeasuredAtFairValueTable_c8e35a22-0006-4aa5-b651-19021408132e" xlink:to="loc_srt_RangeAxis_a0413c06-abd9-47f1-9c9a-14f65b345aa6" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_a0413c06-abd9-47f1-9c9a-14f65b345aa6_default" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_a0413c06-abd9-47f1-9c9a-14f65b345aa6" xlink:to="loc_srt_RangeMember_a0413c06-abd9-47f1-9c9a-14f65b345aa6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_86d3df16-264d-42cc-8ac6-e91da4bf7ef5" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_a0413c06-abd9-47f1-9c9a-14f65b345aa6" xlink:to="loc_srt_RangeMember_86d3df16-264d-42cc-8ac6-e91da4bf7ef5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_WeightedAverageMember_9de31317-d5f2-47b1-be8b-ce940baf24c3" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_WeightedAverageMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_86d3df16-264d-42cc-8ac6-e91da4bf7ef5" xlink:to="loc_srt_WeightedAverageMember_9de31317-d5f2-47b1-be8b-ce940baf24c3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_b2524d36-7bf6-488e-80ab-3d5fdeeab837" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_86d3df16-264d-42cc-8ac6-e91da4bf7ef5" xlink:to="loc_srt_MinimumMember_b2524d36-7bf6-488e-80ab-3d5fdeeab837" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_b66b922c-59c5-4f10-9009-d99ee00e91e0" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_86d3df16-264d-42cc-8ac6-e91da4bf7ef5" xlink:to="loc_srt_MaximumMember_b66b922c-59c5-4f10-9009-d99ee00e91e0" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationTechniqueAxis_009a8cd6-4c47-4bfa-a176-824cce0403e4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ValuationTechniqueAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lkfn_ValuationMethodologyAndUnobservableInputsForLevel3AssetsMeasuredAtFairValueTable_c8e35a22-0006-4aa5-b651-19021408132e" xlink:to="loc_us-gaap_ValuationTechniqueAxis_009a8cd6-4c47-4bfa-a176-824cce0403e4" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationTechniqueDomain_009a8cd6-4c47-4bfa-a176-824cce0403e4_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ValuationTechniqueDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ValuationTechniqueAxis_009a8cd6-4c47-4bfa-a176-824cce0403e4" xlink:to="loc_us-gaap_ValuationTechniqueDomain_009a8cd6-4c47-4bfa-a176-824cce0403e4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationTechniqueDomain_47272215-faf8-4817-b885-30ecb0895864" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ValuationTechniqueDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ValuationTechniqueAxis_009a8cd6-4c47-4bfa-a176-824cce0403e4" xlink:to="loc_us-gaap_ValuationTechniqueDomain_47272215-faf8-4817-b885-30ecb0895864" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_ValuationCollateralBasedMeasurementsMember_9950a344-c888-4324-acc4-3184a76a36c1" xlink:href="lkfn-20220930.xsd#lkfn_ValuationCollateralBasedMeasurementsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ValuationTechniqueDomain_47272215-faf8-4817-b885-30ecb0895864" xlink:to="loc_lkfn_ValuationCollateralBasedMeasurementsMember_9950a344-c888-4324-acc4-3184a76a36c1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostApproachValuationTechniqueMember_da3d96af-b313-41f7-a106-ca2dca4369f4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CostApproachValuationTechniqueMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ValuationTechniqueDomain_47272215-faf8-4817-b885-30ecb0895864" xlink:to="loc_us-gaap_CostApproachValuationTechniqueMember_da3d96af-b313-41f7-a106-ca2dca4369f4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputTypeAxis_462dc7dd-c7ca-42a5-836c-80dd6678974c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_MeasurementInputTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lkfn_ValuationMethodologyAndUnobservableInputsForLevel3AssetsMeasuredAtFairValueTable_c8e35a22-0006-4aa5-b651-19021408132e" xlink:to="loc_us-gaap_MeasurementInputTypeAxis_462dc7dd-c7ca-42a5-836c-80dd6678974c" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputTypeDomain_462dc7dd-c7ca-42a5-836c-80dd6678974c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_MeasurementInputTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_MeasurementInputTypeAxis_462dc7dd-c7ca-42a5-836c-80dd6678974c" xlink:to="loc_us-gaap_MeasurementInputTypeDomain_462dc7dd-c7ca-42a5-836c-80dd6678974c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputTypeDomain_28149378-d3ac-4c63-917d-94150573143e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_MeasurementInputTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_MeasurementInputTypeAxis_462dc7dd-c7ca-42a5-836c-80dd6678974c" xlink:to="loc_us-gaap_MeasurementInputTypeDomain_28149378-d3ac-4c63-917d-94150573143e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputDiscountRateMember_b806ba89-4c36-48dd-afa5-4e8f6553eb1c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_MeasurementInputDiscountRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_MeasurementInputTypeDomain_28149378-d3ac-4c63-917d-94150573143e" xlink:to="loc_us-gaap_MeasurementInputDiscountRateMember_b806ba89-4c36-48dd-afa5-4e8f6553eb1c" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.lakeland.com/role/FAIRVALUEDISCLOSURESEstimatedFairValuesAndTheRelatedCarryingValuesOfFinancialInstrumentsDetails" xlink:type="simple" xlink:href="lkfn-20220930.xsd#FAIRVALUEDISCLOSURESEstimatedFairValuesAndTheRelatedCarryingValuesOfFinancialInstrumentsDetails"/>
  <link:definitionLink xlink:role="http://www.lakeland.com/role/FAIRVALUEDISCLOSURESEstimatedFairValuesAndTheRelatedCarryingValuesOfFinancialInstrumentsDetails" xlink:type="extended" id="i63a41ebe7768423882129fda003fc4f9_FAIRVALUEDISCLOSURESEstimatedFairValuesAndTheRelatedCarryingValuesOfFinancialInstrumentsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems_322533d4-b815-4cff-b40c-c981a5c4d2e5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_EstimatedFairValueAndRelatedCarryingValueOfFinancialInstrumentsAbstract_0d6c6023-6e94-465f-b66a-94d45213ab98" xlink:href="lkfn-20220930.xsd#lkfn_EstimatedFairValueAndRelatedCarryingValueOfFinancialInstrumentsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems_322533d4-b815-4cff-b40c-c981a5c4d2e5" xlink:to="loc_lkfn_EstimatedFairValueAndRelatedCarryingValueOfFinancialInstrumentsAbstract_0d6c6023-6e94-465f-b66a-94d45213ab98" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_4675ca43-45fb-4510-945b-143c5f2dbd46" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_EstimatedFairValueAndRelatedCarryingValueOfFinancialInstrumentsAbstract_0d6c6023-6e94-465f-b66a-94d45213ab98" xlink:to="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_4675ca43-45fb-4510-945b-143c5f2dbd46" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_44d36788-b6f7-4b91-8d35-eb9f37f15dc5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CashAndCashEquivalentsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_4675ca43-45fb-4510-945b-143c5f2dbd46" xlink:to="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_44d36788-b6f7-4b91-8d35-eb9f37f15dc5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleExcludingAccruedInterest_2ca70076-f542-479e-91d0-1f92d7c638a0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtSecuritiesAvailableForSaleExcludingAccruedInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_4675ca43-45fb-4510-945b-143c5f2dbd46" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleExcludingAccruedInterest_2ca70076-f542-479e-91d0-1f92d7c638a0" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesFairValue_ebae361c-7cb2-4a8a-b6c5-d2a73c066ef5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_HeldToMaturitySecuritiesFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_4675ca43-45fb-4510-945b-143c5f2dbd46" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesFairValue_ebae361c-7cb2-4a8a-b6c5-d2a73c066ef5" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_c112a277-92b1-4ca4-9bd8-01522dcb4e21" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AvailableForSaleSecuritiesDebtSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_4675ca43-45fb-4510-945b-143c5f2dbd46" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_c112a277-92b1-4ca4-9bd8-01522dcb4e21" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MortgagesHeldForSaleFairValueDisclosure_5f98cdfa-5447-4453-bde9-c858f3c6027c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_MortgagesHeldForSaleFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_4675ca43-45fb-4510-945b-143c5f2dbd46" xlink:to="loc_us-gaap_MortgagesHeldForSaleFairValueDisclosure_5f98cdfa-5447-4453-bde9-c858f3c6027c" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LoansReceivableFairValueDisclosure_b790d63c-db87-4f26-9635-5bbe725388c2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LoansReceivableFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_4675ca43-45fb-4510-945b-143c5f2dbd46" xlink:to="loc_us-gaap_LoansReceivableFairValueDisclosure_b790d63c-db87-4f26-9635-5bbe725388c2" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_MortgageBankingDerivativeAssetsFairValueDisclosure_e9fe87d6-00e6-49e7-bc42-9f94853bae7b" xlink:href="lkfn-20220930.xsd#lkfn_MortgageBankingDerivativeAssetsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_4675ca43-45fb-4510-945b-143c5f2dbd46" xlink:to="loc_lkfn_MortgageBankingDerivativeAssetsFairValueDisclosure_e9fe87d6-00e6-49e7-bc42-9f94853bae7b" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestRateDerivativeAssetsAtFairValue_2acf7b45-e586-4c4c-9c71-b52f03af9a39" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InterestRateDerivativeAssetsAtFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_4675ca43-45fb-4510-945b-143c5f2dbd46" xlink:to="loc_us-gaap_InterestRateDerivativeAssetsAtFairValue_2acf7b45-e586-4c4c-9c71-b52f03af9a39" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_InvestmentInFederalReserveAndFederalHomeLoanBankStockFairValueDisclosure_31d00942-3fb8-4386-980e-f4cf86525e2b" xlink:href="lkfn-20220930.xsd#lkfn_InvestmentInFederalReserveAndFederalHomeLoanBankStockFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_4675ca43-45fb-4510-945b-143c5f2dbd46" xlink:to="loc_lkfn_InvestmentInFederalReserveAndFederalHomeLoanBankStockFairValueDisclosure_31d00942-3fb8-4386-980e-f4cf86525e2b" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_AccruedInterestReceivableFairValue_8808ddde-15cf-4fed-8d62-9ee1ebc86588" xlink:href="lkfn-20220930.xsd#lkfn_AccruedInterestReceivableFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_4675ca43-45fb-4510-945b-143c5f2dbd46" xlink:to="loc_lkfn_AccruedInterestReceivableFairValue_8808ddde-15cf-4fed-8d62-9ee1ebc86588" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_5b13869d-78eb-4bf7-9334-e20ca663d269" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_EstimatedFairValueAndRelatedCarryingValueOfFinancialInstrumentsAbstract_0d6c6023-6e94-465f-b66a-94d45213ab98" xlink:to="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_5b13869d-78eb-4bf7-9334-e20ca663d269" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_CertificatesOfDepositFairValue_56225dc0-4b0b-4a4f-bd62-6b50e73d4bbd" xlink:href="lkfn-20220930.xsd#lkfn_CertificatesOfDepositFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_5b13869d-78eb-4bf7-9334-e20ca663d269" xlink:to="loc_lkfn_CertificatesOfDepositFairValue_56225dc0-4b0b-4a4f-bd62-6b50e73d4bbd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_AllOtherDepositsFairValue_f790b5ce-60db-4848-99d5-cb6e5f76edcd" xlink:href="lkfn-20220930.xsd#lkfn_AllOtherDepositsFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_5b13869d-78eb-4bf7-9334-e20ca663d269" xlink:to="loc_lkfn_AllOtherDepositsFairValue_f790b5ce-60db-4848-99d5-cb6e5f76edcd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FederalHomeLoanBankBorrowingsFairValueDisclosure_199581fe-d393-4fa0-aa36-d5af079e9896" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FederalHomeLoanBankBorrowingsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_5b13869d-78eb-4bf7-9334-e20ca663d269" xlink:to="loc_us-gaap_FederalHomeLoanBankBorrowingsFairValueDisclosure_199581fe-d393-4fa0-aa36-d5af079e9896" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_MortgageBankingDerivativeLiabilitiesFairValueDisclosure_a73478ee-c57f-470e-814f-90021511e841" xlink:href="lkfn-20220930.xsd#lkfn_MortgageBankingDerivativeLiabilitiesFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_5b13869d-78eb-4bf7-9334-e20ca663d269" xlink:to="loc_lkfn_MortgageBankingDerivativeLiabilitiesFairValueDisclosure_a73478ee-c57f-470e-814f-90021511e841" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestRateDerivativeLiabilitiesAtFairValue_61c44754-d7f5-4cc9-8ea6-7f7cd2d34ed8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InterestRateDerivativeLiabilitiesAtFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_5b13869d-78eb-4bf7-9334-e20ca663d269" xlink:to="loc_us-gaap_InterestRateDerivativeLiabilitiesAtFairValue_61c44754-d7f5-4cc9-8ea6-7f7cd2d34ed8" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommitmentsFairValueDisclosure_d9f8ac8f-eaf2-42a4-aff4-85a23551824f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommitmentsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_5b13869d-78eb-4bf7-9334-e20ca663d269" xlink:to="loc_us-gaap_CommitmentsFairValueDisclosure_d9f8ac8f-eaf2-42a4-aff4-85a23551824f" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_AccruedInterestPayableFairValueCurrent_91b2df47-75f7-426b-af9c-038f0b421c50" xlink:href="lkfn-20220930.xsd#lkfn_AccruedInterestPayableFairValueCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_5b13869d-78eb-4bf7-9334-e20ca663d269" xlink:to="loc_lkfn_AccruedInterestPayableFairValueCurrent_91b2df47-75f7-426b-af9c-038f0b421c50" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByBalanceSheetGroupingTable_530c6e2b-a647-4c65-945c-6f1d492d7652" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueByBalanceSheetGroupingTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems_322533d4-b815-4cff-b40c-c981a5c4d2e5" xlink:to="loc_us-gaap_FairValueByBalanceSheetGroupingTable_530c6e2b-a647-4c65-945c-6f1d492d7652" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_41c7b809-43f6-464f-9d3b-07f75b76e434" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueByBalanceSheetGroupingTable_530c6e2b-a647-4c65-945c-6f1d492d7652" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_41c7b809-43f6-464f-9d3b-07f75b76e434" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_41c7b809-43f6-464f-9d3b-07f75b76e434_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_41c7b809-43f6-464f-9d3b-07f75b76e434" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_41c7b809-43f6-464f-9d3b-07f75b76e434_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_89c86c2d-bb03-4272-bc0e-acba57283143" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_41c7b809-43f6-464f-9d3b-07f75b76e434" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_89c86c2d-bb03-4272-bc0e-acba57283143" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_287fa063-f8db-4462-aaea-9e10d3219769" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_89c86c2d-bb03-4272-bc0e-acba57283143" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_287fa063-f8db-4462-aaea-9e10d3219769" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_4d04455f-7570-4bd6-89ba-199d58c2e1f5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_89c86c2d-bb03-4272-bc0e-acba57283143" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_4d04455f-7570-4bd6-89ba-199d58c2e1f5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_44d7b52d-eb00-4729-8b90-fe8b740fa4e3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueInputsLevel3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_89c86c2d-bb03-4272-bc0e-acba57283143" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_44d7b52d-eb00-4729-8b90-fe8b740fa4e3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementBasisAxis_97dd6822-8fe8-4cf8-b0b7-deedc4a788e5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueByMeasurementBasisAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueByBalanceSheetGroupingTable_530c6e2b-a647-4c65-945c-6f1d492d7652" xlink:to="loc_us-gaap_FairValueByMeasurementBasisAxis_97dd6822-8fe8-4cf8-b0b7-deedc4a788e5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_97dd6822-8fe8-4cf8-b0b7-deedc4a788e5_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_97dd6822-8fe8-4cf8-b0b7-deedc4a788e5" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_97dd6822-8fe8-4cf8-b0b7-deedc4a788e5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueDisclosureItemAmountsDomain_8f423c05-680e-4db1-88e5-8515c34acdd2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FairValueDisclosureItemAmountsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_97dd6822-8fe8-4cf8-b0b7-deedc4a788e5" xlink:to="loc_us-gaap_FairValueDisclosureItemAmountsDomain_8f423c05-680e-4db1-88e5-8515c34acdd2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_08938931-44dd-490b-9d9f-9e59bf3e1b7c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueDisclosureItemAmountsDomain_8f423c05-680e-4db1-88e5-8515c34acdd2" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_08938931-44dd-490b-9d9f-9e59bf3e1b7c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CarryingReportedAmountFairValueDisclosureMember_81c9684a-4f67-4ed8-a98e-b08fabc1985f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CarryingReportedAmountFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueDisclosureItemAmountsDomain_8f423c05-680e-4db1-88e5-8515c34acdd2" xlink:to="loc_us-gaap_CarryingReportedAmountFairValueDisclosureMember_81c9684a-4f67-4ed8-a98e-b08fabc1985f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EstimateOfFairValueFairValueDisclosureMember_86cead7a-3bdd-448c-aae5-ba98535cf53f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EstimateOfFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueDisclosureItemAmountsDomain_8f423c05-680e-4db1-88e5-8515c34acdd2" xlink:to="loc_us-gaap_EstimateOfFairValueFairValueDisclosureMember_86cead7a-3bdd-448c-aae5-ba98535cf53f" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.lakeland.com/role/OFFSETTINGASSETSANDLIABILITIESScheduleofOffsettingofAssetsandLiabilitiesDetails" xlink:type="simple" xlink:href="lkfn-20220930.xsd#OFFSETTINGASSETSANDLIABILITIESScheduleofOffsettingofAssetsandLiabilitiesDetails"/>
  <link:definitionLink xlink:role="http://www.lakeland.com/role/OFFSETTINGASSETSANDLIABILITIESScheduleofOffsettingofAssetsandLiabilitiesDetails" xlink:type="extended" id="i323f3bc710714868ad07027430210331_OFFSETTINGASSETSANDLIABILITIESScheduleofOffsettingofAssetsandLiabilitiesDetails">
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_OffsettingAssetsAndLiabilitiesLineItems_be85ca92-d441-436d-a35c-aa3d7c3f32ad" xlink:href="lkfn-20220930.xsd#lkfn_OffsettingAssetsAndLiabilitiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_OffsettingDerivativeAssetAbstract_0270fc0f-6517-4d9b-8462-90765788220d" xlink:href="lkfn-20220930.xsd#lkfn_OffsettingDerivativeAssetAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_OffsettingAssetsAndLiabilitiesLineItems_be85ca92-d441-436d-a35c-aa3d7c3f32ad" xlink:to="loc_lkfn_OffsettingDerivativeAssetAbstract_0270fc0f-6517-4d9b-8462-90765788220d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeFairValueOfDerivativeAsset_d478a6e6-eea1-4263-85ce-4f88783d21c9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DerivativeFairValueOfDerivativeAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_OffsettingDerivativeAssetAbstract_0270fc0f-6517-4d9b-8462-90765788220d" xlink:to="loc_us-gaap_DerivativeFairValueOfDerivativeAsset_d478a6e6-eea1-4263-85ce-4f88783d21c9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeAssetFairValueGrossLiability_e6e937cc-a0ba-47c7-97f4-7852ca0eeb16" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DerivativeAssetFairValueGrossLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_OffsettingDerivativeAssetAbstract_0270fc0f-6517-4d9b-8462-90765788220d" xlink:to="loc_us-gaap_DerivativeAssetFairValueGrossLiability_e6e937cc-a0ba-47c7-97f4-7852ca0eeb16" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeAssets_1ef6f9ea-e70d-4100-b5d7-13e05c9fb7d4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DerivativeAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_OffsettingDerivativeAssetAbstract_0270fc0f-6517-4d9b-8462-90765788220d" xlink:to="loc_us-gaap_DerivativeAssets_1ef6f9ea-e70d-4100-b5d7-13e05c9fb7d4" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeCollateralObligationToReturnSecurities_82d0bd75-8255-4057-ae21-53cd2ff40a13" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DerivativeCollateralObligationToReturnSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_OffsettingDerivativeAssetAbstract_0270fc0f-6517-4d9b-8462-90765788220d" xlink:to="loc_us-gaap_DerivativeCollateralObligationToReturnSecurities_82d0bd75-8255-4057-ae21-53cd2ff40a13" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeCollateralObligationToReturnCash_95550986-424f-4094-b73e-140cbf088a59" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DerivativeCollateralObligationToReturnCash"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_OffsettingDerivativeAssetAbstract_0270fc0f-6517-4d9b-8462-90765788220d" xlink:to="loc_us-gaap_DerivativeCollateralObligationToReturnCash_95550986-424f-4094-b73e-140cbf088a59" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeFairValueOfDerivativeAssetAmountOffsetAgainstCollateral_20cd1f2d-6d61-49af-9d63-747e7995962b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DerivativeFairValueOfDerivativeAssetAmountOffsetAgainstCollateral"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_OffsettingDerivativeAssetAbstract_0270fc0f-6517-4d9b-8462-90765788220d" xlink:to="loc_us-gaap_DerivativeFairValueOfDerivativeAssetAmountOffsetAgainstCollateral_20cd1f2d-6d61-49af-9d63-747e7995962b" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_OffsettingDerivativesLiabilitiesAbstract_9a0bbc7a-d38c-48bf-9411-53179db6b45a" xlink:href="lkfn-20220930.xsd#lkfn_OffsettingDerivativesLiabilitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_OffsettingAssetsAndLiabilitiesLineItems_be85ca92-d441-436d-a35c-aa3d7c3f32ad" xlink:to="loc_lkfn_OffsettingDerivativesLiabilitiesAbstract_9a0bbc7a-d38c-48bf-9411-53179db6b45a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeFairValueOfDerivativeLiability_eed74252-1ef4-40e9-95e0-37221d480625" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DerivativeFairValueOfDerivativeLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_OffsettingDerivativesLiabilitiesAbstract_9a0bbc7a-d38c-48bf-9411-53179db6b45a" xlink:to="loc_us-gaap_DerivativeFairValueOfDerivativeLiability_eed74252-1ef4-40e9-95e0-37221d480625" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeLiabilityFairValueGrossAsset_832cb56a-ed4f-4a3e-a76a-8f7898999b36" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DerivativeLiabilityFairValueGrossAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_OffsettingDerivativesLiabilitiesAbstract_9a0bbc7a-d38c-48bf-9411-53179db6b45a" xlink:to="loc_us-gaap_DerivativeLiabilityFairValueGrossAsset_832cb56a-ed4f-4a3e-a76a-8f7898999b36" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeLiabilities_3e7064c2-2c69-4464-8917-e5cae44ef1fd" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DerivativeLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_OffsettingDerivativesLiabilitiesAbstract_9a0bbc7a-d38c-48bf-9411-53179db6b45a" xlink:to="loc_us-gaap_DerivativeLiabilities_3e7064c2-2c69-4464-8917-e5cae44ef1fd" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeCollateralRightToReclaimSecurities_69824215-a25f-4a4b-b8a6-9546a883f637" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DerivativeCollateralRightToReclaimSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_OffsettingDerivativesLiabilitiesAbstract_9a0bbc7a-d38c-48bf-9411-53179db6b45a" xlink:to="loc_us-gaap_DerivativeCollateralRightToReclaimSecurities_69824215-a25f-4a4b-b8a6-9546a883f637" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeCollateralRightToReclaimCash_cee240b1-22f4-48b4-9ba1-d4ca1219132e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DerivativeCollateralRightToReclaimCash"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_OffsettingDerivativesLiabilitiesAbstract_9a0bbc7a-d38c-48bf-9411-53179db6b45a" xlink:to="loc_us-gaap_DerivativeCollateralRightToReclaimCash_cee240b1-22f4-48b4-9ba1-d4ca1219132e" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeFairValueOfDerivativeLiabilityAmountOffsetAgainstCollateral_f22b5fc4-6247-402a-a139-2ab7291a1b55" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DerivativeFairValueOfDerivativeLiabilityAmountOffsetAgainstCollateral"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_lkfn_OffsettingDerivativesLiabilitiesAbstract_9a0bbc7a-d38c-48bf-9411-53179db6b45a" xlink:to="loc_us-gaap_DerivativeFairValueOfDerivativeLiabilityAmountOffsetAgainstCollateral_f22b5fc4-6247-402a-a139-2ab7291a1b55" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_OffsettingAssetsAndLiabilitiesTable_f7dcbac4-9981-40e2-87a8-080748583d7e" xlink:href="lkfn-20220930.xsd#lkfn_OffsettingAssetsAndLiabilitiesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_lkfn_OffsettingAssetsAndLiabilitiesLineItems_be85ca92-d441-436d-a35c-aa3d7c3f32ad" xlink:to="loc_lkfn_OffsettingAssetsAndLiabilitiesTable_f7dcbac4-9981-40e2-87a8-080748583d7e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeInstrumentRiskAxis_04fc72ab-400f-463b-91b7-d8208e418521" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DerivativeInstrumentRiskAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_lkfn_OffsettingAssetsAndLiabilitiesTable_f7dcbac4-9981-40e2-87a8-080748583d7e" xlink:to="loc_us-gaap_DerivativeInstrumentRiskAxis_04fc72ab-400f-463b-91b7-d8208e418521" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_04fc72ab-400f-463b-91b7-d8208e418521_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_04fc72ab-400f-463b-91b7-d8208e418521" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_04fc72ab-400f-463b-91b7-d8208e418521_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_29ef6823-675d-40b8-9569-dc284b341406" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_04fc72ab-400f-463b-91b7-d8208e418521" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_29ef6823-675d-40b8-9569-dc284b341406" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestRateSwapMember_35c622b3-b450-4dc7-a735-28db73563d9b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InterestRateSwapMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeContractTypeDomain_29ef6823-675d-40b8-9569-dc284b341406" xlink:to="loc_us-gaap_InterestRateSwapMember_35c622b3-b450-4dc7-a735-28db73563d9b" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.lakeland.com/role/ACCUMULATEDOTHERCOMPREHENSIVEINCOMEChangesinAccumulatedOtherComprehensiveIncomeDetails" xlink:type="simple" xlink:href="lkfn-20220930.xsd#ACCUMULATEDOTHERCOMPREHENSIVEINCOMEChangesinAccumulatedOtherComprehensiveIncomeDetails"/>
  <link:definitionLink xlink:role="http://www.lakeland.com/role/ACCUMULATEDOTHERCOMPREHENSIVEINCOMEChangesinAccumulatedOtherComprehensiveIncomeDetails" xlink:type="extended" id="i1fbbaffb11e84f25ace47c89f437327e_ACCUMULATEDOTHERCOMPREHENSIVEINCOMEChangesinAccumulatedOtherComprehensiveIncomeDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_ed6c3fd1-9b0d-402c-a917-4472c9dfb027" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodNetOfTaxAbstract_5b2b2073-214b-4058-ab82-a46b6f726d8a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodNetOfTaxAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_ed6c3fd1-9b0d-402c-a917-4472c9dfb027" xlink:to="loc_us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodNetOfTaxAbstract_5b2b2073-214b-4058-ab82-a46b6f726d8a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_9d92e310-88e8-42f7-9609-1a10aad69fe5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodNetOfTaxAbstract_5b2b2073-214b-4058-ab82-a46b6f726d8a" xlink:to="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_9d92e310-88e8-42f7-9609-1a10aad69fe5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossBeforeReclassificationsNetOfTax_46b099d5-df54-4775-bb06-e56d329ae7f5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherComprehensiveIncomeLossBeforeReclassificationsNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodNetOfTaxAbstract_5b2b2073-214b-4058-ab82-a46b6f726d8a" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossBeforeReclassificationsNetOfTax_46b099d5-df54-4775-bb06-e56d329ae7f5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodNetOfTax_079141de-2393-49ba-a2e2-13e47367eebb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodNetOfTaxAbstract_5b2b2073-214b-4058-ab82-a46b6f726d8a" xlink:to="loc_us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodNetOfTax_079141de-2393-49ba-a2e2-13e47367eebb" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_f4ed2c54-14d2-4b7c-ab22-994a141a383c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodNetOfTaxAbstract_5b2b2073-214b-4058-ab82-a46b6f726d8a" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_f4ed2c54-14d2-4b7c-ab22-994a141a383c" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_d96bfc5c-558b-4c59-8f0c-d0054ed3ea78" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_ca707b56-7137-48a3-9cd4-dff604a871e6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_ed6c3fd1-9b0d-402c-a917-4472c9dfb027" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_ca707b56-7137-48a3-9cd4-dff604a871e6" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_2bd6e871-7d01-4fe9-abe5-0cdefd22a286" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_ca707b56-7137-48a3-9cd4-dff604a871e6" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_2bd6e871-7d01-4fe9-abe5-0cdefd22a286" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_2bd6e871-7d01-4fe9-abe5-0cdefd22a286_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_2bd6e871-7d01-4fe9-abe5-0cdefd22a286" xlink:to="loc_us-gaap_EquityComponentDomain_2bd6e871-7d01-4fe9-abe5-0cdefd22a286_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_c135c56e-0f87-4a57-a257-e87dfd9a79fd" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_2bd6e871-7d01-4fe9-abe5-0cdefd22a286" xlink:to="loc_us-gaap_EquityComponentDomain_c135c56e-0f87-4a57-a257-e87dfd9a79fd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AociIncludingPortionAttributableToNoncontrollingInterestMember_cf0dc779-7006-4a43-bb3b-1c883d12c5a6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AociIncludingPortionAttributableToNoncontrollingInterestMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_c135c56e-0f87-4a57-a257-e87dfd9a79fd" xlink:to="loc_us-gaap_AociIncludingPortionAttributableToNoncontrollingInterestMember_cf0dc779-7006-4a43-bb3b-1c883d12c5a6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedNetInvestmentGainLossIncludingPortionAttributableToNoncontrollingInterestMember_b735b5f3-ed90-4c45-afe5-f5f4a7f4d246" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccumulatedNetInvestmentGainLossIncludingPortionAttributableToNoncontrollingInterestMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AociIncludingPortionAttributableToNoncontrollingInterestMember_cf0dc779-7006-4a43-bb3b-1c883d12c5a6" xlink:to="loc_us-gaap_AccumulatedNetInvestmentGainLossIncludingPortionAttributableToNoncontrollingInterestMember_b735b5f3-ed90-4c45-afe5-f5f4a7f4d246" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDefinedBenefitPlansAdjustmentIncludingPortionAttributableToNoncontrollingInterestMember_c1159e80-0c00-4058-be82-c7e7ab086a79" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccumulatedDefinedBenefitPlansAdjustmentIncludingPortionAttributableToNoncontrollingInterestMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AociIncludingPortionAttributableToNoncontrollingInterestMember_cf0dc779-7006-4a43-bb3b-1c883d12c5a6" xlink:to="loc_us-gaap_AccumulatedDefinedBenefitPlansAdjustmentIncludingPortionAttributableToNoncontrollingInterestMember_c1159e80-0c00-4058-be82-c7e7ab086a79" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.lakeland.com/role/ACCUMULATEDOTHERCOMPREHENSIVEINCOMEReclassificationsDetails" xlink:type="simple" xlink:href="lkfn-20220930.xsd#ACCUMULATEDOTHERCOMPREHENSIVEINCOMEReclassificationsDetails"/>
  <link:definitionLink xlink:role="http://www.lakeland.com/role/ACCUMULATEDOTHERCOMPREHENSIVEINCOMEReclassificationsDetails" xlink:type="extended" id="i72ff60caaa7043d98a05f9d884f5e3fa_ACCUMULATEDOTHERCOMPREHENSIVEINCOMEReclassificationsDetails">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReclassificationAdjustmentOutOfAccumulatedOtherComprehensiveIncomeLineItems_11a3ec0c-41c2-400b-b2be-3aa4187a9716" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ReclassificationAdjustmentOutOfAccumulatedOtherComprehensiveIncomeLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestAndDividendIncomeOperating_f30ca559-a52a-4aa8-bc0b-f4828609a57b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InterestAndDividendIncomeOperating"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReclassificationAdjustmentOutOfAccumulatedOtherComprehensiveIncomeLineItems_11a3ec0c-41c2-400b-b2be-3aa4187a9716" xlink:to="loc_us-gaap_InterestAndDividendIncomeOperating_f30ca559-a52a-4aa8-bc0b-f4828609a57b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtAndEquitySecuritiesGainLoss_03e1049b-c59d-4a95-9531-17f890a96056" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DebtAndEquitySecuritiesGainLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReclassificationAdjustmentOutOfAccumulatedOtherComprehensiveIncomeLineItems_11a3ec0c-41c2-400b-b2be-3aa4187a9716" xlink:to="loc_us-gaap_DebtAndEquitySecuritiesGainLoss_03e1049b-c59d-4a95-9531-17f890a96056" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_cac97443-5295-4f53-8bc4-3756ef78aa17" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncomeTaxExpenseBenefit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReclassificationAdjustmentOutOfAccumulatedOtherComprehensiveIncomeLineItems_11a3ec0c-41c2-400b-b2be-3aa4187a9716" xlink:to="loc_us-gaap_IncomeTaxExpenseBenefit_cac97443-5295-4f53-8bc4-3756ef78aa17" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNoninterestExpense_c4a4d550-0fa1-4441-b2f4-51c8df3ac7f9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherNoninterestExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReclassificationAdjustmentOutOfAccumulatedOtherComprehensiveIncomeLineItems_11a3ec0c-41c2-400b-b2be-3aa4187a9716" xlink:to="loc_us-gaap_OtherNoninterestExpense_c4a4d550-0fa1-4441-b2f4-51c8df3ac7f9" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_ce94e684-ae8a-4b8a-b028-615b00fb2692" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReclassificationAdjustmentOutOfAccumulatedOtherComprehensiveIncomeLineItems_11a3ec0c-41c2-400b-b2be-3aa4187a9716" xlink:to="loc_us-gaap_NetIncomeLoss_ce94e684-ae8a-4b8a-b028-615b00fb2692" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReclassificationOutOfAccumulatedOtherComprehensiveIncomeTable_03c64c2b-a3b5-480e-a58c-8c0d9e22f59e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ReclassificationOutOfAccumulatedOtherComprehensiveIncomeTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ReclassificationAdjustmentOutOfAccumulatedOtherComprehensiveIncomeLineItems_11a3ec0c-41c2-400b-b2be-3aa4187a9716" xlink:to="loc_us-gaap_ReclassificationOutOfAccumulatedOtherComprehensiveIncomeTable_03c64c2b-a3b5-480e-a58c-8c0d9e22f59e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReclassificationOutOfAccumulatedOtherComprehensiveIncomeAxis_caaa06ba-e72c-4f56-9c96-a5d418665be1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ReclassificationOutOfAccumulatedOtherComprehensiveIncomeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ReclassificationOutOfAccumulatedOtherComprehensiveIncomeTable_03c64c2b-a3b5-480e-a58c-8c0d9e22f59e" xlink:to="loc_us-gaap_ReclassificationOutOfAccumulatedOtherComprehensiveIncomeAxis_caaa06ba-e72c-4f56-9c96-a5d418665be1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReclassificationOutOfAccumulatedOtherComprehensiveIncomeDomain_caaa06ba-e72c-4f56-9c96-a5d418665be1_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ReclassificationOutOfAccumulatedOtherComprehensiveIncomeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ReclassificationOutOfAccumulatedOtherComprehensiveIncomeAxis_caaa06ba-e72c-4f56-9c96-a5d418665be1" xlink:to="loc_us-gaap_ReclassificationOutOfAccumulatedOtherComprehensiveIncomeDomain_caaa06ba-e72c-4f56-9c96-a5d418665be1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReclassificationOutOfAccumulatedOtherComprehensiveIncomeDomain_d3fd4e6e-46b3-4708-a12d-e9541fd704f8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ReclassificationOutOfAccumulatedOtherComprehensiveIncomeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ReclassificationOutOfAccumulatedOtherComprehensiveIncomeAxis_caaa06ba-e72c-4f56-9c96-a5d418665be1" xlink:to="loc_us-gaap_ReclassificationOutOfAccumulatedOtherComprehensiveIncomeDomain_d3fd4e6e-46b3-4708-a12d-e9541fd704f8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReclassificationOutOfAccumulatedOtherComprehensiveIncomeMember_10062ee0-e5db-44ca-b340-ca26f3aae25f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ReclassificationOutOfAccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReclassificationOutOfAccumulatedOtherComprehensiveIncomeDomain_d3fd4e6e-46b3-4708-a12d-e9541fd704f8" xlink:to="loc_us-gaap_ReclassificationOutOfAccumulatedOtherComprehensiveIncomeMember_10062ee0-e5db-44ca-b340-ca26f3aae25f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_7115bbcd-01c4-46c7-8228-0bfd2ee0e3be" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ReclassificationOutOfAccumulatedOtherComprehensiveIncomeTable_03c64c2b-a3b5-480e-a58c-8c0d9e22f59e" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_7115bbcd-01c4-46c7-8228-0bfd2ee0e3be" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_7115bbcd-01c4-46c7-8228-0bfd2ee0e3be_default" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_7115bbcd-01c4-46c7-8228-0bfd2ee0e3be" xlink:to="loc_us-gaap_EquityComponentDomain_7115bbcd-01c4-46c7-8228-0bfd2ee0e3be_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_c22e75ef-344f-49e2-8fb8-466e1d97ce1c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_7115bbcd-01c4-46c7-8228-0bfd2ee0e3be" xlink:to="loc_us-gaap_EquityComponentDomain_c22e75ef-344f-49e2-8fb8-466e1d97ce1c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_lkfn_AOCIAccumulatedGainLossDebtSecuritiesHeldToMaturityParentMember_26115aa9-3874-4fca-bc74-b4b857412226" xlink:href="lkfn-20220930.xsd#lkfn_AOCIAccumulatedGainLossDebtSecuritiesHeldToMaturityParentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_c22e75ef-344f-49e2-8fb8-466e1d97ce1c" xlink:to="loc_lkfn_AOCIAccumulatedGainLossDebtSecuritiesHeldToMaturityParentMember_26115aa9-3874-4fca-bc74-b4b857412226" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedNetUnrealizedInvestmentGainLossMember_a5a7b6d8-5965-4553-a67e-63d53def2a38" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccumulatedNetUnrealizedInvestmentGainLossMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_c22e75ef-344f-49e2-8fb8-466e1d97ce1c" xlink:to="loc_us-gaap_AccumulatedNetUnrealizedInvestmentGainLossMember_a5a7b6d8-5965-4553-a67e-63d53def2a38" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDefinedBenefitPlansAdjustmentMember_6077a5ac-3589-4a3c-9cdc-309113e73fa6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccumulatedDefinedBenefitPlansAdjustmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_c22e75ef-344f-49e2-8fb8-466e1d97ce1c" xlink:to="loc_us-gaap_AccumulatedDefinedBenefitPlansAdjustmentMember_6077a5ac-3589-4a3c-9cdc-309113e73fa6" xlink:type="arc" order="2"/>
  </link:definitionLink>
</link:linkbase>
