Exhibit 16.1

March 25, 2022

U.S. Securities and Exchange Commission

Office of the Chief Accountant

100 F Street, NE

Washington, DC 20549

Re: Innoviva, Inc.

File No. 000-30319

Dear Sir or Madam:

We have read Item 4.01 of Form 8-K of Innoviva, Inc. dated March 25, 2022, and agree with the statements concerning our Firm contained therein.

Very truly yours,

/s/ GRANT THORNTON LLP