EX-32.1 17 ea023044501ex32-1_orukathe.htm CERTIFICATION

Exhibit 32.1

Certifications of the

Principal Executive Officer and Principal Financial Officer

Pursuant To 18 U.S.C. Section 1350,

As Adopted Pursuant To

Section 906 of The Sarbanes-Oxley Act Of 2002

In connection with the Annual Report on Form 10-K of Oruka Therapeutics, Inc. (the “Company”) for the year ended December 31, 2024, as filed with the Securities and Exchange Commission on the date hereof (the “Report”), each of the undersigned officers of the Company hereby certifies, pursuant to 18 U.S.C. Section 1350, as adopted pursuant to Section 906 of the Sarbanes-Oxley Act of 2002, that to the best of his knowledge:

1) | The Report fully complies with the requirements of Section 13(a) or 15(d) of the Securities Exchange Act of 1934; and

2) | The information contained in the Report fairly presents, in all material respects, the financial condition and results of operations of the Company.

Date: March 6, 2025 | /s/ Lawrence Klein
| Lawrence Klein
| President and Chief Executive Officer
| (Principal Executive Officer)
| /s/ Arjun Agarwal
| Arjun Agarwal
| Senior Vice President, Finance and Treasurer
| (Principal Financial Officer and Principal Accounting Officer)