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STATEMENTS OF CASH FLOWS - USD ($) $ in Thousands | 12 Months Ended
--- | ---
Dec. 31, 2021 | Dec. 31, 2020 | Dec. 31, 2019
Cash flows from operating activities: |  |  |
Net loss | $ (116,112) | $ (75,617) | $ (68,548)
Adjustments to reconcile net loss to net cash used in operating activities: |  |  |
Depreciation and amortization | 215 | 158 | 64
Accretion and amortization on investments, net | 1,424 | 818 | (1,534)
Amortization of debt issuance costs/debt discount | 893 | 179 |
Gain on sales of available for sale securities |  | (19) |
Net gain on exchange and sales of equity investment | (1,233) | (148) |
Change in fair value of equity investment, including foreign currency translation |  | (163) | 196
Stock-based compensation for services by non-employees | 91 | 85 | 68
Stock-based compensation for employees and directors | 8,080 | 6,895 | 6,079
Amortization related to 401(k) contributions |  |  | 9
Amortization of right-of-use assets | 568 | 777 | 712
Changes in assets and liabilities: |  |  |
Interest and other receivables | (1,041) | 80 | 366
Prepaid and other current assets | 1,317 | (1,286) | 121
Deposit and other assets | (3,807) | (206) | (964)
Accounts payable | (232) | 5,731 | 199
Accrued compensation and benefits | (119) | 3,388 | 2,188
Amount due to Janssen Biotech, Inc. |  | (14,269) | 11,659
Accrued liabilities | 14,909 | 7,397 | 6,211
Operating lease liabilities | (509) | (452) | (655)
Net cash used in operating activities | (95,556) | (66,652) | (43,829)
Cash flows from investing activities: |  |  |
Purchases of property and equipment | (207) | (401) | (413)
Purchases of marketable securities | (177,434) | (313,201) | (153,467)
Proceeds from sales of securities available for sale |  | 7,681 |
Proceeds from maturities of marketable securities | 247,994 | 200,262 | 181,280
Proceeds from sales of equity investment | 1,594 | 339 |
Net cash provided by (used in) investing activities | 71,947 | (105,320) | 27,400
Cash flows from financing activities: |  |  |
Proceeds from issuances of common stock from equity plans | 797 | 208 | 204
Proceeds from issuance of common stock and warrants in public offering, net of paid issuance costs |  | 140,184 |
Proceeds from issuances of common stock from at market offerings, net of paid issuance costs | 20,385 | 4,075 | 19,295
Proceeds from exercise of warrants | 2,479 | 16 |
Proceeds from debt financing, net of paid debt issuance costs and debt discounts | 24,895 | 23,863 |
Net cash provided by financing activities | 48,556 | 168,346 | 19,499
Net increase (decrease) in cash, cash equivalents and restricted cash | 24,947 | (3,626) | 3,070
Cash, cash equivalents and restricted cash at the beginning of the period | 10,288 | 13,914 | 10,844
Cash, cash equivalents and restricted cash at the end of the period | $ 35,235 | $ 10,288 | $ 13,914

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