<SEC-DOCUMENT>0001193125-23-205187-index.html : 20230808
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<ACCEPTANCE-DATETIME>20230807193836
ACCESSION NUMBER:		0001193125-23-205187
CONFORMED SUBMISSION TYPE:	424B5
PUBLIC DOCUMENT COUNT:		3
FILED AS OF DATE:		20230808
DATE AS OF CHANGE:		20230807

FILER:

	COMPANY DATA:	
		COMPANY CONFORMED NAME:			GLADSTONE INVESTMENT CORPORATION\DE
		CENTRAL INDEX KEY:			0001321741
		IRS NUMBER:				830423116
		STATE OF INCORPORATION:			DE
		FISCAL YEAR END:			0331

	FILING VALUES:
		FORM TYPE:		424B5
		SEC ACT:		1933 Act
		SEC FILE NUMBER:	333-259302
		FILM NUMBER:		231148968

	BUSINESS ADDRESS:	
		STREET 1:		1521 WESTBRANCH DRIVE
		STREET 2:		SUITE 100
		CITY:			MCLEAN
		STATE:			VA
		ZIP:			22102
		BUSINESS PHONE:		7032875800

	MAIL ADDRESS:	
		STREET 1:		1521 WESTBRANCH DRIVE
		STREET 2:		SUITE 100
		CITY:			MCLEAN
		STATE:			VA
		ZIP:			22102
</SEC-HEADER>
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<TYPE>424B5
<SEQUENCE>1
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<DESCRIPTION>424B5
<TEXT>
Document 1 - file: d507312d424b5.htm

</DOCUMENT>
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<TYPE>GRAPHIC
<SEQUENCE>2
<FILENAME>g507312g50q21.jpg
<DESCRIPTION>GRAPHIC
<TEXT>
Document 2 - file: g507312g50q21.jpg

</DOCUMENT>
<DOCUMENT>
<TYPE>GRAPHIC
<SEQUENCE>3
<FILENAME>g507312g96z92.jpg
<DESCRIPTION>GRAPHIC
<TEXT>
Document 3 - file: g507312g96z92.jpg

</DOCUMENT>
</SEC-DOCUMENT>