<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with Wdesk from Workiva-->
<!--Copyright 2020 Workiva-->
<!--r:cc860809-a6b3-4939-9e89-fb60809a6371,g:7453d80d-f328-4339-bf18-c087f9945f8a-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:roleRef roleURI="http://fluidigm.com/role/Cover" xlink:type="simple" xlink:href="fldm-20200930.xsd#Cover"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/Cover" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/CondensedConsolidatedBalanceSheets" xlink:type="simple" xlink:href="fldm-20200930.xsd#CondensedConsolidatedBalanceSheets"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/CondensedConsolidatedBalanceSheets" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquity_81b83608-9633-404a-8629-f5925df7f7c7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LiabilitiesAndStockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_b88d3337-87ed-408e-8887-6e677a90d720" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_Liabilities"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_81b83608-9633-404a-8629-f5925df7f7c7" xlink:to="loc_us-gaap_Liabilities_b88d3337-87ed-408e-8887-6e677a90d720" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_e233af31-be41-451d-9572-20fa992431f0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockholdersEquity"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_81b83608-9633-404a-8629-f5925df7f7c7" xlink:to="loc_us-gaap_StockholdersEquity_e233af31-be41-451d-9572-20fa992431f0" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommitmentsAndContingencies_5e60375e-29e4-456f-8807-bd5f44e5d55b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommitmentsAndContingencies"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_81b83608-9633-404a-8629-f5925df7f7c7" xlink:to="loc_us-gaap_CommitmentsAndContingencies_5e60375e-29e4-456f-8807-bd5f44e5d55b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Assets_7be4be1d-e3a2-4d70-956e-8a684430c8fd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_Assets"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_14b3ded5-d9d4-4367-96f7-8ead3690444b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AssetsCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_7be4be1d-e3a2-4d70-956e-8a684430c8fd" xlink:to="loc_us-gaap_AssetsCurrent_14b3ded5-d9d4-4367-96f7-8ead3690444b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_b301dbcd-3341-4685-8235-7afe4602bd7a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_7be4be1d-e3a2-4d70-956e-8a684430c8fd" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_b301dbcd-3341-4685-8235-7afe4602bd7a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseRightOfUseAsset_c3e5f9ca-7963-4c56-8c3f-52519a8f78b5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingLeaseRightOfUseAsset"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_7be4be1d-e3a2-4d70-956e-8a684430c8fd" xlink:to="loc_us-gaap_OperatingLeaseRightOfUseAsset_c3e5f9ca-7963-4c56-8c3f-52519a8f78b5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsNoncurrent_a8ddfe67-55bc-4659-a626-9e2fb54ba7d0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherAssetsNoncurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_7be4be1d-e3a2-4d70-956e-8a684430c8fd" xlink:to="loc_us-gaap_OtherAssetsNoncurrent_a8ddfe67-55bc-4659-a626-9e2fb54ba7d0" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IntangibleAssetsNetExcludingGoodwill_adb346ee-737d-41bc-9cac-26427c491748" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IntangibleAssetsNetExcludingGoodwill"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_7be4be1d-e3a2-4d70-956e-8a684430c8fd" xlink:to="loc_us-gaap_IntangibleAssetsNetExcludingGoodwill_adb346ee-737d-41bc-9cac-26427c491748" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Goodwill_f1bffda1-0e07-4c23-a63d-e39934df032b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_Goodwill"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_7be4be1d-e3a2-4d70-956e-8a684430c8fd" xlink:to="loc_us-gaap_Goodwill_f1bffda1-0e07-4c23-a63d-e39934df032b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_df033b21-cefd-4cd8-9ad6-aef534bd0170" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_Liabilities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilitiesNoncurrent_0faf005b-0574-4a76-92f7-7d7a64200b20" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherLiabilitiesNoncurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_df033b21-cefd-4cd8-9ad6-aef534bd0170" xlink:to="loc_us-gaap_OtherLiabilitiesNoncurrent_0faf005b-0574-4a76-92f7-7d7a64200b20" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityNoncurrent_4793d35d-34c5-435f-bf12-3298cd6b9fda" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ContractWithCustomerLiabilityNoncurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_df033b21-cefd-4cd8-9ad6-aef534bd0170" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityNoncurrent_4793d35d-34c5-435f-bf12-3298cd6b9fda" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredIncomeTaxLiabilitiesNet_2c2b441c-7463-450a-849d-1075cc690e85" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DeferredIncomeTaxLiabilitiesNet"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_df033b21-cefd-4cd8-9ad6-aef534bd0170" xlink:to="loc_us-gaap_DeferredIncomeTaxLiabilitiesNet_2c2b441c-7463-450a-849d-1075cc690e85" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConvertibleLongTermNotesPayable_d385136b-d7f6-43e7-be2c-380f680dc3b6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ConvertibleLongTermNotesPayable"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_df033b21-cefd-4cd8-9ad6-aef534bd0170" xlink:to="loc_us-gaap_ConvertibleLongTermNotesPayable_d385136b-d7f6-43e7-be2c-380f680dc3b6" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_775a7826-e22f-47d3-870a-de38595fe9bd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LiabilitiesCurrent"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_df033b21-cefd-4cd8-9ad6-aef534bd0170" xlink:to="loc_us-gaap_LiabilitiesCurrent_775a7826-e22f-47d3-870a-de38595fe9bd" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_fldm_DeferredGrantRevenueNoncurrent_cf48e88e-4e7f-4cb2-8e04-25850e7abc15" xlink:href="fldm-20200930.xsd#fldm_DeferredGrantRevenueNoncurrent"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_df033b21-cefd-4cd8-9ad6-aef534bd0170" xlink:to="loc_fldm_DeferredGrantRevenueNoncurrent_cf48e88e-4e7f-4cb2-8e04-25850e7abc15" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_81061482-17ad-4562-86f4-5d2affa4317b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingLeaseLiabilityNoncurrent"/>
    <link:calculationArc order="7" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_df033b21-cefd-4cd8-9ad6-aef534bd0170" xlink:to="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_81061482-17ad-4562-86f4-5d2affa4317b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_29a919ec-dace-4a18-bc04-4e8b34921656" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LiabilitiesCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsPayableCurrent_d73ac6dd-b43f-4951-881c-34c737830836" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccountsPayableCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_29a919ec-dace-4a18-bc04-4e8b34921656" xlink:to="loc_us-gaap_AccountsPayableCurrent_d73ac6dd-b43f-4951-881c-34c737830836" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeRelatedLiabilitiesCurrent_36b84f37-53b4-4a0c-befd-05185634ad8d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EmployeeRelatedLiabilitiesCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_29a919ec-dace-4a18-bc04-4e8b34921656" xlink:to="loc_us-gaap_EmployeeRelatedLiabilitiesCurrent_36b84f37-53b4-4a0c-befd-05185634ad8d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityCurrent_56c1ce4d-f4ec-4659-89fd-a7a5c6caf063" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingLeaseLiabilityCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_29a919ec-dace-4a18-bc04-4e8b34921656" xlink:to="loc_us-gaap_OperatingLeaseLiabilityCurrent_56c1ce4d-f4ec-4659-89fd-a7a5c6caf063" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccruedLiabilitiesCurrent_627cebec-c54a-4916-8ec2-826e2caa868e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccruedLiabilitiesCurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_29a919ec-dace-4a18-bc04-4e8b34921656" xlink:to="loc_us-gaap_AccruedLiabilitiesCurrent_627cebec-c54a-4916-8ec2-826e2caa868e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityCurrent_de89d11d-e6a2-4f46-a509-ec630585da40" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ContractWithCustomerLiabilityCurrent"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_29a919ec-dace-4a18-bc04-4e8b34921656" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityCurrent_de89d11d-e6a2-4f46-a509-ec630585da40" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_a15e9610-671d-4b4a-93ad-31a153627ee8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_StockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockValue_f98e5cbf-4192-446a-95f0-3ff9af7b3b24" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PreferredStockValue"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_a15e9610-671d-4b4a-93ad-31a153627ee8" xlink:to="loc_us-gaap_PreferredStockValue_f98e5cbf-4192-446a-95f0-3ff9af7b3b24" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockValue_4232c5aa-8ce3-4db7-a4ab-9cda1aa31d69" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CommonStockValue"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_a15e9610-671d-4b4a-93ad-31a153627ee8" xlink:to="loc_us-gaap_CommonStockValue_4232c5aa-8ce3-4db7-a4ab-9cda1aa31d69" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapital_968d871a-2b3a-4f34-8fdf-91392de3e2c9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AdditionalPaidInCapital"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_a15e9610-671d-4b4a-93ad-31a153627ee8" xlink:to="loc_us-gaap_AdditionalPaidInCapital_968d871a-2b3a-4f34-8fdf-91392de3e2c9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_d010c39b-4c11-4c1f-b671-dbed285c0fa7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_a15e9610-671d-4b4a-93ad-31a153627ee8" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_d010c39b-4c11-4c1f-b671-dbed285c0fa7" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsAccumulatedDeficit_785c69d7-d0da-4266-8047-20a60763c252" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RetainedEarningsAccumulatedDeficit"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_a15e9610-671d-4b4a-93ad-31a153627ee8" xlink:to="loc_us-gaap_RetainedEarningsAccumulatedDeficit_785c69d7-d0da-4266-8047-20a60763c252" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_55fa4527-1ad5-4799-9773-008d9333f961" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AssetsCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_b4c6c3c4-ea49-49a6-823b-d57f8b48d2d6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_55fa4527-1ad5-4799-9773-008d9333f961" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_b4c6c3c4-ea49-49a6-823b-d57f8b48d2d6" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShortTermInvestments_8d1c8bf0-e7f5-4576-9e8b-620093719d17" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShortTermInvestments"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_55fa4527-1ad5-4799-9773-008d9333f961" xlink:to="loc_us-gaap_ShortTermInvestments_8d1c8bf0-e7f5-4576-9e8b-620093719d17" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsReceivableNetCurrent_2f7bcdd6-9a5f-4269-96b9-d9242754fc0a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccountsReceivableNetCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_55fa4527-1ad5-4799-9773-008d9333f961" xlink:to="loc_us-gaap_AccountsReceivableNetCurrent_2f7bcdd6-9a5f-4269-96b9-d9242754fc0a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryNet_0557f19f-a1ab-4329-9547-1f9af9d78c16" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_InventoryNet"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_55fa4527-1ad5-4799-9773-008d9333f961" xlink:to="loc_us-gaap_InventoryNet_0557f19f-a1ab-4329-9547-1f9af9d78c16" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_5884697a-f6d0-497b-9ec8-26146ee14715" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PrepaidExpenseAndOtherAssetsCurrent"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_55fa4527-1ad5-4799-9773-008d9333f961" xlink:to="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_5884697a-f6d0-497b-9ec8-26146ee14715" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_fldm_ResearchAndDevelopmentArrangementReceivables_e54d2f4b-682e-4c06-b2d9-fd22de5eacf2" xlink:href="fldm-20200930.xsd#fldm_ResearchAndDevelopmentArrangementReceivables"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_55fa4527-1ad5-4799-9773-008d9333f961" xlink:to="loc_fldm_ResearchAndDevelopmentArrangementReceivables_e54d2f4b-682e-4c06-b2d9-fd22de5eacf2" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://fluidigm.com/role/CondensedConsolidatedBalanceSheetsParenthetical" xlink:type="simple" xlink:href="fldm-20200930.xsd#CondensedConsolidatedBalanceSheetsParenthetical"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/CondensedConsolidatedBalanceSheetsParenthetical" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/CondensedConsolidatedStatementsofOperations" xlink:type="simple" xlink:href="fldm-20200930.xsd#CondensedConsolidatedStatementsofOperations"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/CondensedConsolidatedStatementsofOperations" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_2c1e80fe-8315-4767-b8b3-a5adf914d7f6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingExpenses_c40c934e-5d40-4cde-bf61-cfdf9e0bce32" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingExpenses"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingIncomeLoss_2c1e80fe-8315-4767-b8b3-a5adf914d7f6" xlink:to="loc_us-gaap_OperatingExpenses_c40c934e-5d40-4cde-bf61-cfdf9e0bce32" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_69fcbb0b-17ee-495f-981b-61aaf307a16d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingIncomeLoss_2c1e80fe-8315-4767-b8b3-a5adf914d7f6" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_69fcbb0b-17ee-495f-981b-61aaf307a16d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_c8d06c35-7b97-4332-aa63-d1536bb4b95f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NetIncomeLoss"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesMinorityInterestAndIncomeLossFromEquityMethodInvestments_0fc31211-bfba-4710-b39d-884247f19aa5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesMinorityInterestAndIncomeLossFromEquityMethodInvestments"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_c8d06c35-7b97-4332-aa63-d1536bb4b95f" xlink:to="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesMinorityInterestAndIncomeLossFromEquityMethodInvestments_0fc31211-bfba-4710-b39d-884247f19aa5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_47ca0150-96cf-4e99-9160-9942140ccf1b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeTaxExpenseBenefit"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_c8d06c35-7b97-4332-aa63-d1536bb4b95f" xlink:to="loc_us-gaap_IncomeTaxExpenseBenefit_47ca0150-96cf-4e99-9160-9942140ccf1b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingExpenses_fe0364d8-9484-49c5-855a-a0603f08e686" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingExpenses"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpense_e54b686f-feb2-4765-86ea-b6677b835b36" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ResearchAndDevelopmentExpense"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingExpenses_fe0364d8-9484-49c5-855a-a0603f08e686" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpense_e54b686f-feb2-4765-86ea-b6677b835b36" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingGeneralAndAdministrativeExpense_982344b5-0641-47bc-b861-8af98cac8ab5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_SellingGeneralAndAdministrativeExpense"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingExpenses_fe0364d8-9484-49c5-855a-a0603f08e686" xlink:to="loc_us-gaap_SellingGeneralAndAdministrativeExpense_982344b5-0641-47bc-b861-8af98cac8ab5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfGoodsAndServicesSold_2418b9b9-4e97-4387-a96e-a9c592e4c2ef" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CostOfGoodsAndServicesSold"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingExpenses_fe0364d8-9484-49c5-855a-a0603f08e686" xlink:to="loc_us-gaap_CostOfGoodsAndServicesSold_2418b9b9-4e97-4387-a96e-a9c592e4c2ef" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesMinorityInterestAndIncomeLossFromEquityMethodInvestments_8f08273a-fe28-4aa8-a712-31910a28f892" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesMinorityInterestAndIncomeLossFromEquityMethodInvestments"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_d616bbe9-e7e8-4074-835e-f0f955fe6cd9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesMinorityInterestAndIncomeLossFromEquityMethodInvestments_8f08273a-fe28-4aa8-a712-31910a28f892" xlink:to="loc_us-gaap_OperatingIncomeLoss_d616bbe9-e7e8-4074-835e-f0f955fe6cd9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpense_d737c914-72f2-4ff4-9f05-dcdebcabefca" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_InterestExpense"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesMinorityInterestAndIncomeLossFromEquityMethodInvestments_8f08273a-fe28-4aa8-a712-31910a28f892" xlink:to="loc_us-gaap_InterestExpense_d737c914-72f2-4ff4-9f05-dcdebcabefca" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GainsLossesOnExtinguishmentOfDebt_429744ee-902d-457e-a455-fa92de84b0a2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_GainsLossesOnExtinguishmentOfDebt"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesMinorityInterestAndIncomeLossFromEquityMethodInvestments_8f08273a-fe28-4aa8-a712-31910a28f892" xlink:to="loc_us-gaap_GainsLossesOnExtinguishmentOfDebt_429744ee-902d-457e-a455-fa92de84b0a2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNonoperatingIncomeExpense_5da53ffe-bc11-40f7-9501-c623b7814cab" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherNonoperatingIncomeExpense"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesMinorityInterestAndIncomeLossFromEquityMethodInvestments_8f08273a-fe28-4aa8-a712-31910a28f892" xlink:to="loc_us-gaap_OtherNonoperatingIncomeExpense_5da53ffe-bc11-40f7-9501-c623b7814cab" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://fluidigm.com/role/CondensedConsolidatedStatementsofComprehensiveLoss" xlink:type="simple" xlink:href="fldm-20200930.xsd#CondensedConsolidatedStatementsofComprehensiveLoss"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/CondensedConsolidatedStatementsofComprehensiveLoss" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ComprehensiveIncomeNetOfTax_3ae8e623-a666-4d1b-907f-63711cf0ce4b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ComprehensiveIncomeNetOfTax"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_73bb5e66-116a-492b-b61e-a1e6ac452686" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NetIncomeLoss"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_3ae8e623-a666-4d1b-907f-63711cf0ce4b" xlink:to="loc_us-gaap_NetIncomeLoss_73bb5e66-116a-492b-b61e-a1e6ac452686" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_447a13f9-5802-4b17-90c9-749ae89c37e4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_3ae8e623-a666-4d1b-907f-63711cf0ce4b" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_447a13f9-5802-4b17-90c9-749ae89c37e4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_be0a61f4-f288-4d8e-ba5e-b94109e37b7e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossForeignCurrencyTransactionAndTranslationAdjustmentNetOfTax_3bd95286-8783-4579-b5b6-876728c5cb67" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherComprehensiveIncomeLossForeignCurrencyTransactionAndTranslationAdjustmentNetOfTax"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_be0a61f4-f288-4d8e-ba5e-b94109e37b7e" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossForeignCurrencyTransactionAndTranslationAdjustmentNetOfTax_3bd95286-8783-4579-b5b6-876728c5cb67" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeUnrealizedHoldingGainLossOnSecuritiesArisingDuringPeriodNetOfTax_f6180fd5-5dcb-49a5-a538-648dab4655c9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherComprehensiveIncomeUnrealizedHoldingGainLossOnSecuritiesArisingDuringPeriodNetOfTax"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_be0a61f4-f288-4d8e-ba5e-b94109e37b7e" xlink:to="loc_us-gaap_OtherComprehensiveIncomeUnrealizedHoldingGainLossOnSecuritiesArisingDuringPeriodNetOfTax_f6180fd5-5dcb-49a5-a538-648dab4655c9" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://fluidigm.com/role/CondensedConsolidatedStatementsofStockholdersEquity" xlink:type="simple" xlink:href="fldm-20200930.xsd#CondensedConsolidatedStatementsofStockholdersEquity"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/CondensedConsolidatedStatementsofStockholdersEquity" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/CondensedConsolidatedStatementsofCashFlows" xlink:type="simple" xlink:href="fldm-20200930.xsd#CondensedConsolidatedStatementsofCashFlows"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/CondensedConsolidatedStatementsofCashFlows" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_188fa096-8c2e-4e22-ad5d-c17da111ecf0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_fldm_ProceedsFromResearchAndDevelopmentGrant_cb2cb348-9760-42d7-866c-628f17372ec0" xlink:href="fldm-20200930.xsd#fldm_ProceedsFromResearchAndDevelopmentGrant"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_188fa096-8c2e-4e22-ad5d-c17da111ecf0" xlink:to="loc_fldm_ProceedsFromResearchAndDevelopmentGrant_cb2cb348-9760-42d7-866c-628f17372ec0" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromMaturitiesPrepaymentsAndCallsOfOtherInvestments_335e8950-9244-4846-88b5-e42d365debe9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ProceedsFromMaturitiesPrepaymentsAndCallsOfOtherInvestments"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_188fa096-8c2e-4e22-ad5d-c17da111ecf0" xlink:to="loc_us-gaap_ProceedsFromMaturitiesPrepaymentsAndCallsOfOtherInvestments_335e8950-9244-4846-88b5-e42d365debe9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireBusinessesNetOfCashAcquired_917bb4a1-c460-47e1-985a-dd815ae78911" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PaymentsToAcquireBusinessesNetOfCashAcquired"/>
    <link:calculationArc order="3" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_188fa096-8c2e-4e22-ad5d-c17da111ecf0" xlink:to="loc_us-gaap_PaymentsToAcquireBusinessesNetOfCashAcquired_917bb4a1-c460-47e1-985a-dd815ae78911" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireInvestments_100040a5-5775-4ff7-a459-e275103a482c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PaymentsToAcquireInvestments"/>
    <link:calculationArc order="4" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_188fa096-8c2e-4e22-ad5d-c17da111ecf0" xlink:to="loc_us-gaap_PaymentsToAcquireInvestments_100040a5-5775-4ff7-a459-e275103a482c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromSaleOfOtherInvestments_78669b91-3ec8-4eb8-8f22-36a13d6e2097" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ProceedsFromSaleOfOtherInvestments"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_188fa096-8c2e-4e22-ad5d-c17da111ecf0" xlink:to="loc_us-gaap_ProceedsFromSaleOfOtherInvestments_78669b91-3ec8-4eb8-8f22-36a13d6e2097" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_bbd7bb29-28fe-4fa6-a7bb-feeba71dfd7d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PaymentsToAcquirePropertyPlantAndEquipment"/>
    <link:calculationArc order="6" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_188fa096-8c2e-4e22-ad5d-c17da111ecf0" xlink:to="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_bbd7bb29-28fe-4fa6-a7bb-feeba71dfd7d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_00d99246-09d0-40f1-b2f7-57088b5c7498" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsRelatedToTaxWithholdingForShareBasedCompensation_ace3ef8e-2521-439e-b659-4e366d5b2062" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PaymentsRelatedToTaxWithholdingForShareBasedCompensation"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_00d99246-09d0-40f1-b2f7-57088b5c7498" xlink:to="loc_us-gaap_PaymentsRelatedToTaxWithholdingForShareBasedCompensation_ace3ef8e-2521-439e-b659-4e366d5b2062" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromStockOptionsExercised_b2a491dd-e290-4f34-a96f-8d8110d1aece" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ProceedsFromStockOptionsExercised"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_00d99246-09d0-40f1-b2f7-57088b5c7498" xlink:to="loc_us-gaap_ProceedsFromStockOptionsExercised_b2a491dd-e290-4f34-a96f-8d8110d1aece" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOfSharesUnderIncentiveAndShareBasedCompensationPlans_c9a6362d-8877-404b-94c7-14d489702213" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ProceedsFromIssuanceOfSharesUnderIncentiveAndShareBasedCompensationPlans"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_00d99246-09d0-40f1-b2f7-57088b5c7498" xlink:to="loc_us-gaap_ProceedsFromIssuanceOfSharesUnderIncentiveAndShareBasedCompensationPlans_c9a6362d-8877-404b-94c7-14d489702213" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentOfFinancingAndStockIssuanceCosts_6276e651-08b9-42dd-9779-81a98b4dce83" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PaymentOfFinancingAndStockIssuanceCosts"/>
    <link:calculationArc order="4" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_00d99246-09d0-40f1-b2f7-57088b5c7498" xlink:to="loc_us-gaap_PaymentOfFinancingAndStockIssuanceCosts_6276e651-08b9-42dd-9779-81a98b4dce83" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOfCommonStock_1aaea681-72da-498e-bbfc-90a3c7011624" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ProceedsFromIssuanceOfCommonStock"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_00d99246-09d0-40f1-b2f7-57088b5c7498" xlink:to="loc_us-gaap_ProceedsFromIssuanceOfCommonStock_1aaea681-72da-498e-bbfc-90a3c7011624" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_adbc2d6d-836d-4e3a-af03-c93855d92132" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_485a9023-d7e7-4449-99c1-5167d14f26ca" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NetIncomeLoss"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_adbc2d6d-836d-4e3a-af03-c93855d92132" xlink:to="loc_us-gaap_NetIncomeLoss_485a9023-d7e7-4449-99c1-5167d14f26ca" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepreciationAmortizationAndAccretionNet_35a4178a-1de1-4380-bce6-301e3c1b7b69" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DepreciationAmortizationAndAccretionNet"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_adbc2d6d-836d-4e3a-af03-c93855d92132" xlink:to="loc_us-gaap_DepreciationAmortizationAndAccretionNet_35a4178a-1de1-4380-bce6-301e3c1b7b69" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensation_d1da45a8-a05b-4787-ad21-0b548249faec" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShareBasedCompensation"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_adbc2d6d-836d-4e3a-af03-c93855d92132" xlink:to="loc_us-gaap_ShareBasedCompensation_d1da45a8-a05b-4787-ad21-0b548249faec" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AmortizationOfIntangibleAssets_c91422e0-4b97-4983-b5c2-7268d7d84e57" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AmortizationOfIntangibleAssets"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_adbc2d6d-836d-4e3a-af03-c93855d92132" xlink:to="loc_us-gaap_AmortizationOfIntangibleAssets_c91422e0-4b97-4983-b5c2-7268d7d84e57" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GainsLossesOnExtinguishmentOfDebt_7c608363-23fa-4927-9896-400022023c5c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_GainsLossesOnExtinguishmentOfDebt"/>
    <link:calculationArc order="5" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_adbc2d6d-836d-4e3a-af03-c93855d92132" xlink:to="loc_us-gaap_GainsLossesOnExtinguishmentOfDebt_7c608363-23fa-4927-9896-400022023c5c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherOperatingActivitiesCashFlowStatement_e6738336-75db-423e-b7a1-389385b5eb80" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OtherOperatingActivitiesCashFlowStatement"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_adbc2d6d-836d-4e3a-af03-c93855d92132" xlink:to="loc_us-gaap_OtherOperatingActivitiesCashFlowStatement_e6738336-75db-423e-b7a1-389385b5eb80" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GainLossOnSaleOfPropertyPlantEquipment_00f3b08e-e8d0-4a3e-ac99-7b09cffbb570" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_GainLossOnSaleOfPropertyPlantEquipment"/>
    <link:calculationArc order="7" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_adbc2d6d-836d-4e3a-af03-c93855d92132" xlink:to="loc_us-gaap_GainLossOnSaleOfPropertyPlantEquipment_00f3b08e-e8d0-4a3e-ac99-7b09cffbb570" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsReceivable_1e110808-5086-4031-8ee9-efdfe0ca3aca" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncreaseDecreaseInAccountsReceivable"/>
    <link:calculationArc order="8" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_adbc2d6d-836d-4e3a-af03-c93855d92132" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsReceivable_1e110808-5086-4031-8ee9-efdfe0ca3aca" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInInventories_6bb5944c-1bd9-4451-b0d4-37e8742af74f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncreaseDecreaseInInventories"/>
    <link:calculationArc order="9" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_adbc2d6d-836d-4e3a-af03-c93855d92132" xlink:to="loc_us-gaap_IncreaseDecreaseInInventories_6bb5944c-1bd9-4451-b0d4-37e8742af74f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets_30ae7987-c235-450f-9930-0e6694321a8e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets"/>
    <link:calculationArc order="10" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_adbc2d6d-836d-4e3a-af03-c93855d92132" xlink:to="loc_us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets_30ae7987-c235-450f-9930-0e6694321a8e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsPayable_3a5af918-edb3-4cf2-bf2f-cc01b59244a7" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncreaseDecreaseInAccountsPayable"/>
    <link:calculationArc order="11" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_adbc2d6d-836d-4e3a-af03-c93855d92132" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsPayable_3a5af918-edb3-4cf2-bf2f-cc01b59244a7" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInContractWithCustomerLiability_a1cd4119-9bc5-4578-a1ed-7783ed9d120a" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncreaseDecreaseInContractWithCustomerLiability"/>
    <link:calculationArc order="12" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_adbc2d6d-836d-4e3a-af03-c93855d92132" xlink:to="loc_us-gaap_IncreaseDecreaseInContractWithCustomerLiability_a1cd4119-9bc5-4578-a1ed-7783ed9d120a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInOtherOperatingLiabilities_71ff59b6-8e82-45b0-8b83-89cf259ba44f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_IncreaseDecreaseInOtherOperatingLiabilities"/>
    <link:calculationArc order="13" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_adbc2d6d-836d-4e3a-af03-c93855d92132" xlink:to="loc_us-gaap_IncreaseDecreaseInOtherOperatingLiabilities_71ff59b6-8e82-45b0-8b83-89cf259ba44f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AmortizationOfFinancingCostsAndDiscounts_83f8f0f2-0787-4130-ba9d-bb53d9273fbf" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AmortizationOfFinancingCostsAndDiscounts"/>
    <link:calculationArc order="14" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_adbc2d6d-836d-4e3a-af03-c93855d92132" xlink:to="loc_us-gaap_AmortizationOfFinancingCostsAndDiscounts_83f8f0f2-0787-4130-ba9d-bb53d9273fbf" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryLIFOReservePeriodCharge_621f5014-91de-4355-bbb2-d00672e7af38" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_InventoryLIFOReservePeriodCharge"/>
    <link:calculationArc order="15" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_adbc2d6d-836d-4e3a-af03-c93855d92132" xlink:to="loc_us-gaap_InventoryLIFOReservePeriodCharge_621f5014-91de-4355-bbb2-d00672e7af38" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseRightOfUseAssetAmortizationExpense_e25074dc-c744-45d2-92bb-877f6a74480b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingLeaseRightOfUseAssetAmortizationExpense"/>
    <link:calculationArc order="16" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_adbc2d6d-836d-4e3a-af03-c93855d92132" xlink:to="loc_us-gaap_OperatingLeaseRightOfUseAssetAmortizationExpense_e25074dc-c744-45d2-92bb-877f6a74480b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_7782080c-e580-461a-88bd-209b445914a6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_814c37af-9c38-43b1-9c0b-51cc4e0fe745" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_7782080c-e580-461a-88bd-209b445914a6" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_814c37af-9c38-43b1-9c0b-51cc4e0fe745" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_3edfe96a-c82f-4dde-a741-fda5134aa2e8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_7782080c-e580-461a-88bd-209b445914a6" xlink:to="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_3edfe96a-c82f-4dde-a741-fda5134aa2e8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_91baad8a-e3ec-4222-9178-b39222e98741" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_7782080c-e580-461a-88bd-209b445914a6" xlink:to="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_91baad8a-e3ec-4222-9178-b39222e98741" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EffectOfExchangeRateOnCashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_e14e5c89-5383-4df7-81ce-1a86dfdbd76d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_EffectOfExchangeRateOnCashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_7782080c-e580-461a-88bd-209b445914a6" xlink:to="loc_us-gaap_EffectOfExchangeRateOnCashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_e14e5c89-5383-4df7-81ce-1a86dfdbd76d" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://fluidigm.com/role/DescriptionofBusiness" xlink:type="simple" xlink:href="fldm-20200930.xsd#DescriptionofBusiness"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/DescriptionofBusiness" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/SummaryofSignificantAccountingPolicies" xlink:type="simple" xlink:href="fldm-20200930.xsd#SummaryofSignificantAccountingPolicies"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/SummaryofSignificantAccountingPolicies" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/SummaryofSignificantAccountingPoliciesPolicies" xlink:type="simple" xlink:href="fldm-20200930.xsd#SummaryofSignificantAccountingPoliciesPolicies"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/SummaryofSignificantAccountingPoliciesPolicies" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/SummaryofSignificantAccountingPoliciesTables" xlink:type="simple" xlink:href="fldm-20200930.xsd#SummaryofSignificantAccountingPoliciesTables"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/SummaryofSignificantAccountingPoliciesTables" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/SummaryofSignificantAccountingPoliciesNarrativeDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#SummaryofSignificantAccountingPoliciesNarrativeDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/SummaryofSignificantAccountingPoliciesNarrativeDetails" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/SummaryofSignificantAccountingPoliciesAccumulatedOtherComprehensiveLossDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#SummaryofSignificantAccountingPoliciesAccumulatedOtherComprehensiveLossDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/SummaryofSignificantAccountingPoliciesAccumulatedOtherComprehensiveLossDetails" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/SummaryofSignificantAccountingPoliciesPotentialCommonSharesExcludedfromComputationsofDilutedNetLossPerShareDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#SummaryofSignificantAccountingPoliciesPotentialCommonSharesExcludedfromComputationsofDilutedNetLossPerShareDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/SummaryofSignificantAccountingPoliciesPotentialCommonSharesExcludedfromComputationsofDilutedNetLossPerShareDetails" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/BusinessCombination" xlink:type="simple" xlink:href="fldm-20200930.xsd#BusinessCombination"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/BusinessCombination" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/BusinessCombinationTables" xlink:type="simple" xlink:href="fldm-20200930.xsd#BusinessCombinationTables"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/BusinessCombinationTables" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/BusinessCombinationNarrativeDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#BusinessCombinationNarrativeDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/BusinessCombinationNarrativeDetails" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/BusinessCombinationNetCashFlowsDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#BusinessCombinationNetCashFlowsDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/BusinessCombinationNetCashFlowsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireBusinessesNetOfCashAcquired_93d9b69a-ca0f-427f-9c61-ed2b26c06927" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PaymentsToAcquireBusinessesNetOfCashAcquired"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireBusinessesGross_b42b21c5-ca7f-43e0-802e-eb81d3c7c385" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PaymentsToAcquireBusinessesGross"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PaymentsToAcquireBusinessesNetOfCashAcquired_93d9b69a-ca0f-427f-9c61-ed2b26c06927" xlink:to="loc_us-gaap_PaymentsToAcquireBusinessesGross_b42b21c5-ca7f-43e0-802e-eb81d3c7c385" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAcquiredFromAcquisition_93b47b3f-72d8-435d-9a96-26f505dc07f9" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CashAcquiredFromAcquisition"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PaymentsToAcquireBusinessesNetOfCashAcquired_93d9b69a-ca0f-427f-9c61-ed2b26c06927" xlink:to="loc_us-gaap_CashAcquiredFromAcquisition_93b47b3f-72d8-435d-9a96-26f505dc07f9" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://fluidigm.com/role/BusinessCombinationScheduleofConsiderationTransferredandIdentifiableAssetsandLiabilitiesDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#BusinessCombinationScheduleofConsiderationTransferredandIdentifiableAssetsandLiabilitiesDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/BusinessCombinationScheduleofConsiderationTransferredandIdentifiableAssetsandLiabilitiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNet_d0a5be19-f37b-41bf-89ea-7ccf6db31afd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCashAndEquivalents_4e770b9c-0ab5-406c-944f-08aea47abafb" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCashAndEquivalents"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNet_d0a5be19-f37b-41bf-89ea-7ccf6db31afd" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCashAndEquivalents_4e770b9c-0ab5-406c-944f-08aea47abafb" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_fldm_BusinessCombinationRecognizedIdentifiableAssetsAcquiredandLiabilitiesAssumedAccountsReceivables_366c99e1-9cce-46f9-9a56-587a711207c0" xlink:href="fldm-20200930.xsd#fldm_BusinessCombinationRecognizedIdentifiableAssetsAcquiredandLiabilitiesAssumedAccountsReceivables"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNet_d0a5be19-f37b-41bf-89ea-7ccf6db31afd" xlink:to="loc_fldm_BusinessCombinationRecognizedIdentifiableAssetsAcquiredandLiabilitiesAssumedAccountsReceivables_366c99e1-9cce-46f9-9a56-587a711207c0" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_fldm_BusinessCombinationRecognizedIdentifiableAssetsAcquiredandLiabilitiesAssumedOtherReceivables_e0550350-ff09-4ea1-b255-f3e9381a009e" xlink:href="fldm-20200930.xsd#fldm_BusinessCombinationRecognizedIdentifiableAssetsAcquiredandLiabilitiesAssumedOtherReceivables"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNet_d0a5be19-f37b-41bf-89ea-7ccf6db31afd" xlink:to="loc_fldm_BusinessCombinationRecognizedIdentifiableAssetsAcquiredandLiabilitiesAssumedOtherReceivables_e0550350-ff09-4ea1-b255-f3e9381a009e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedInventory_58f36205-0a05-4cd0-9495-ed8518c97885" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedInventory"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNet_d0a5be19-f37b-41bf-89ea-7ccf6db31afd" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedInventory_58f36205-0a05-4cd0-9495-ed8518c97885" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIndefiniteLivedIntangibleAssets_e80c0db1-2836-4291-bf5c-3a9a6909e3ca" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIndefiniteLivedIntangibleAssets"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNet_d0a5be19-f37b-41bf-89ea-7ccf6db31afd" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIndefiniteLivedIntangibleAssets_e80c0db1-2836-4291-bf5c-3a9a6909e3ca" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesAccountsPayable_d2426d11-4545-47ab-89d1-95656dbb7fd8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesAccountsPayable"/>
    <link:calculationArc order="6" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNet_d0a5be19-f37b-41bf-89ea-7ccf6db31afd" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesAccountsPayable_d2426d11-4545-47ab-89d1-95656dbb7fd8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesOther_964acc9b-e571-49f7-a86e-f4b054ca15e0" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesOther"/>
    <link:calculationArc order="7" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNet_d0a5be19-f37b-41bf-89ea-7ccf6db31afd" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesOther_964acc9b-e571-49f7-a86e-f4b054ca15e0" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedDeferredTaxLiabilities_1911b377-32e3-4ec8-a6d4-7442bdcefa49" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedDeferredTaxLiabilities"/>
    <link:calculationArc order="8" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNet_d0a5be19-f37b-41bf-89ea-7ccf6db31afd" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedDeferredTaxLiabilities_1911b377-32e3-4ec8-a6d4-7442bdcefa49" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationConsiderationTransferred1_cb4d3469-60fb-413f-90c8-d76da8c69409" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationConsiderationTransferred1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireBusinessesGross_8daab7d1-c473-42ad-a31c-ac7ca685224d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PaymentsToAcquireBusinessesGross"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_BusinessCombinationConsiderationTransferred1_cb4d3469-60fb-413f-90c8-d76da8c69409" xlink:to="loc_us-gaap_PaymentsToAcquireBusinessesGross_8daab7d1-c473-42ad-a31c-ac7ca685224d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationConsiderationTransferredEquityInterestsIssuedAndIssuable_9c89b621-0cdd-433a-b292-68c711950ed4" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_BusinessCombinationConsiderationTransferredEquityInterestsIssuedAndIssuable"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_BusinessCombinationConsiderationTransferred1_cb4d3469-60fb-413f-90c8-d76da8c69409" xlink:to="loc_us-gaap_BusinessCombinationConsiderationTransferredEquityInterestsIssuedAndIssuable_9c89b621-0cdd-433a-b292-68c711950ed4" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://fluidigm.com/role/RADxProgram" xlink:type="simple" xlink:href="fldm-20200930.xsd#RADxProgram"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/RADxProgram" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/RADxProgramTables" xlink:type="simple" xlink:href="fldm-20200930.xsd#RADxProgramTables"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/RADxProgramTables" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/RADxProgramDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#RADxProgramDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/RADxProgramDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_fldm_DeferredGrantRevenueNoncurrent_a1933291-076d-4f3a-8b24-1177987a6b1a" xlink:href="fldm-20200930.xsd#fldm_DeferredGrantRevenueNoncurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_fldm_ResearchAndDevelopmentArrangementContractToPerformForOthersAmount_86f7b4d3-c95d-4608-b1b4-4113daaaa292" xlink:href="fldm-20200930.xsd#fldm_ResearchAndDevelopmentArrangementContractToPerformForOthersAmount"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_fldm_DeferredGrantRevenueNoncurrent_a1933291-076d-4f3a-8b24-1177987a6b1a" xlink:to="loc_fldm_ResearchAndDevelopmentArrangementContractToPerformForOthersAmount_86f7b4d3-c95d-4608-b1b4-4113daaaa292" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_fldm_ResearchAndDevelopmentArrangementContractToPerformForOthersExpenses_91340969-cb72-4eac-a7c0-b3636dce3156" xlink:href="fldm-20200930.xsd#fldm_ResearchAndDevelopmentArrangementContractToPerformForOthersExpenses"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_fldm_DeferredGrantRevenueNoncurrent_a1933291-076d-4f3a-8b24-1177987a6b1a" xlink:to="loc_fldm_ResearchAndDevelopmentArrangementContractToPerformForOthersExpenses_91340969-cb72-4eac-a7c0-b3636dce3156" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_fldm_ResearchAndDevelopmentArrangementReceivables_ec05e7d0-6ba5-4553-bfe1-012061ee8a12" xlink:href="fldm-20200930.xsd#fldm_ResearchAndDevelopmentArrangementReceivables"/>
    <link:loc xlink:type="locator" xlink:label="loc_fldm_ResearchAndDevelopmentArrangementContractToPerformForOthersAmount_ccea17bc-bbd9-4f0b-b463-6008fa7064bc" xlink:href="fldm-20200930.xsd#fldm_ResearchAndDevelopmentArrangementContractToPerformForOthersAmount"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_fldm_ResearchAndDevelopmentArrangementReceivables_ec05e7d0-6ba5-4553-bfe1-012061ee8a12" xlink:to="loc_fldm_ResearchAndDevelopmentArrangementContractToPerformForOthersAmount_ccea17bc-bbd9-4f0b-b463-6008fa7064bc" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_fldm_ResearchAndDevelopmentArrangementContractToPerformForOthersFundingReceived_c6d7cc6f-c08b-4e03-abe7-3a26acdf4936" xlink:href="fldm-20200930.xsd#fldm_ResearchAndDevelopmentArrangementContractToPerformForOthersFundingReceived"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_fldm_ResearchAndDevelopmentArrangementReceivables_ec05e7d0-6ba5-4553-bfe1-012061ee8a12" xlink:to="loc_fldm_ResearchAndDevelopmentArrangementContractToPerformForOthersFundingReceived_c6d7cc6f-c08b-4e03-abe7-3a26acdf4936" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://fluidigm.com/role/Revenue" xlink:type="simple" xlink:href="fldm-20200930.xsd#Revenue"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/Revenue" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/RevenueTables" xlink:type="simple" xlink:href="fldm-20200930.xsd#RevenueTables"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/RevenueTables" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/RevenueScheduleofDisaggregationofRevenueDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#RevenueScheduleofDisaggregationofRevenueDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/RevenueScheduleofDisaggregationofRevenueDetails" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/RevenueNarrativeDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#RevenueNarrativeDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/RevenueNarrativeDetails" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/RevenuePerformanceObligationsDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#RevenuePerformanceObligationsDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/RevenuePerformanceObligationsDetails" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/RevenuePerformanceObligationsDetails_1" xlink:type="simple" xlink:href="fldm-20200930.xsd#RevenuePerformanceObligationsDetails_1"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/RevenuePerformanceObligationsDetails_1" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/GoodwillandIntangibleAssetsnet" xlink:type="simple" xlink:href="fldm-20200930.xsd#GoodwillandIntangibleAssetsnet"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/GoodwillandIntangibleAssetsnet" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/GoodwillandIntangibleAssetsnetTables" xlink:type="simple" xlink:href="fldm-20200930.xsd#GoodwillandIntangibleAssetsnetTables"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/GoodwillandIntangibleAssetsnetTables" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/GoodwillandIntangibleAssetsnetNarrativeDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#GoodwillandIntangibleAssetsnetNarrativeDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/GoodwillandIntangibleAssetsnetNarrativeDetails" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/GoodwillandIntangibleAssetsnetScheduleofFinitelivedIntangibleAssetsDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#GoodwillandIntangibleAssetsnetScheduleofFinitelivedIntangibleAssetsDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/GoodwillandIntangibleAssetsnetScheduleofFinitelivedIntangibleAssetsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsNet_0cefc465-03e1-4e00-b2a2-1341f81f645b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsGross_569a0493-71ab-419a-ad5c-6ae3f62ae30f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsGross"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsNet_0cefc465-03e1-4e00-b2a2-1341f81f645b" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsGross_569a0493-71ab-419a-ad5c-6ae3f62ae30f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_88f60879-99cb-4d53-aa1b-2000cd5cb786" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsNet_0cefc465-03e1-4e00-b2a2-1341f81f645b" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_88f60879-99cb-4d53-aa1b-2000cd5cb786" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://fluidigm.com/role/GoodwillandIntangibleAssetsnetFutureAmortizationExpenseDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#GoodwillandIntangibleAssetsnetFutureAmortizationExpenseDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/GoodwillandIntangibleAssetsnetFutureAmortizationExpenseDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsNet_bee94738-60ab-4735-8e42-35311fcf1475" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseRemainderOfFiscalYear_59499d87-bca3-442c-8f5a-0d51f8836ce2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseRemainderOfFiscalYear"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsNet_bee94738-60ab-4735-8e42-35311fcf1475" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseRemainderOfFiscalYear_59499d87-bca3-442c-8f5a-0d51f8836ce2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseNextTwelveMonths_35b434c8-d6ef-42b3-ac1c-4f62ef4c4bdf" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseNextTwelveMonths"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsNet_bee94738-60ab-4735-8e42-35311fcf1475" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseNextTwelveMonths_35b434c8-d6ef-42b3-ac1c-4f62ef4c4bdf" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseYearFour_12613519-ea7e-441a-9076-1976c5db0db1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseYearFour"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsNet_bee94738-60ab-4735-8e42-35311fcf1475" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseYearFour_12613519-ea7e-441a-9076-1976c5db0db1" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseYearTwo_76c47aeb-370f-4921-8f88-a42a3f44bf34" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseYearTwo"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsNet_bee94738-60ab-4735-8e42-35311fcf1475" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseYearTwo_76c47aeb-370f-4921-8f88-a42a3f44bf34" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_fldm_FiniteLivedIntangibleAssetExpectedAmortizationAfterYearFour_6e7570ee-7a57-4209-b583-0637ebf69306" xlink:href="fldm-20200930.xsd#fldm_FiniteLivedIntangibleAssetExpectedAmortizationAfterYearFour"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsNet_bee94738-60ab-4735-8e42-35311fcf1475" xlink:to="loc_fldm_FiniteLivedIntangibleAssetExpectedAmortizationAfterYearFour_6e7570ee-7a57-4209-b583-0637ebf69306" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseYearThree_f893be5e-3223-4faf-94ba-1b90d98c585f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseYearThree"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsNet_bee94738-60ab-4735-8e42-35311fcf1475" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAmortizationExpenseYearThree_f893be5e-3223-4faf-94ba-1b90d98c585f" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://fluidigm.com/role/BalanceSheetDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#BalanceSheetDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/BalanceSheetDetails" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/BalanceSheetDetailsTables" xlink:type="simple" xlink:href="fldm-20200930.xsd#BalanceSheetDetailsTables"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/BalanceSheetDetailsTables" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/BalanceSheetDetailsSummaryofCashandCashEquivalentsDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#BalanceSheetDetailsSummaryofCashandCashEquivalentsDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/BalanceSheetDetailsSummaryofCashandCashEquivalentsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_ce6c344a-6f18-4c7b-af5a-adb964a32016" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_21c34522-df83-4c78-a146-b039d919ee94" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_ce6c344a-6f18-4c7b-af5a-adb964a32016" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_21c34522-df83-4c78-a146-b039d919ee94" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashAndCashEquivalentsAtCarryingValue_9cd28408-6675-4f93-beb3-51efa98b895e" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestrictedCashAndCashEquivalentsAtCarryingValue"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_ce6c344a-6f18-4c7b-af5a-adb964a32016" xlink:to="loc_us-gaap_RestrictedCashAndCashEquivalentsAtCarryingValue_9cd28408-6675-4f93-beb3-51efa98b895e" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://fluidigm.com/role/BalanceSheetDetailsInventoriesDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#BalanceSheetDetailsInventoriesDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/BalanceSheetDetailsInventoriesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryNet_d05dc061-598b-4398-a898-f39a4e3dd769" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_InventoryNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryRawMaterialsNetOfReserves_e7dbce94-9a1e-43ed-957c-890c49ba2fdd" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_InventoryRawMaterialsNetOfReserves"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InventoryNet_d05dc061-598b-4398-a898-f39a4e3dd769" xlink:to="loc_us-gaap_InventoryRawMaterialsNetOfReserves_e7dbce94-9a1e-43ed-957c-890c49ba2fdd" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryWorkInProcessNetOfReserves_37d5205a-79bd-4f85-b40e-1a60f3160d6d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_InventoryWorkInProcessNetOfReserves"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InventoryNet_d05dc061-598b-4398-a898-f39a4e3dd769" xlink:to="loc_us-gaap_InventoryWorkInProcessNetOfReserves_37d5205a-79bd-4f85-b40e-1a60f3160d6d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryFinishedGoodsNetOfReserves_01bdeab8-4559-48f1-a9b0-840a7464e446" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_InventoryFinishedGoodsNetOfReserves"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InventoryNet_d05dc061-598b-4398-a898-f39a4e3dd769" xlink:to="loc_us-gaap_InventoryFinishedGoodsNetOfReserves_01bdeab8-4559-48f1-a9b0-840a7464e446" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://fluidigm.com/role/BalanceSheetDetailsPropertyandEquipmentDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#BalanceSheetDetailsPropertyandEquipmentDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/BalanceSheetDetailsPropertyandEquipmentDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_f4cdb3ba-d1af-4935-9bf8-b4193c1b43f1" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentGross_e8a40d67-54e0-477b-a336-47edea05554c" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_PropertyPlantAndEquipmentGross"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentNet_f4cdb3ba-d1af-4935-9bf8-b4193c1b43f1" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentGross_e8a40d67-54e0-477b-a336-47edea05554c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_4dc3151b-0ff9-48cf-a803-49c125896281" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentNet_f4cdb3ba-d1af-4935-9bf8-b4193c1b43f1" xlink:to="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_4dc3151b-0ff9-48cf-a803-49c125896281" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConstructionInProgressGross_1545ddb3-56a4-4755-8dda-f2fde1bcaaa5" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ConstructionInProgressGross"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentNet_f4cdb3ba-d1af-4935-9bf8-b4193c1b43f1" xlink:to="loc_us-gaap_ConstructionInProgressGross_1545ddb3-56a4-4755-8dda-f2fde1bcaaa5" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://fluidigm.com/role/BalanceSheetDetailsWarrantyAccrualDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#BalanceSheetDetailsWarrantyAccrualDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/BalanceSheetDetailsWarrantyAccrualDetails" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/ConvertibleNotesandCreditFacility" xlink:type="simple" xlink:href="fldm-20200930.xsd#ConvertibleNotesandCreditFacility"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/ConvertibleNotesandCreditFacility" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/ConvertibleNotesandCreditFacilityTables" xlink:type="simple" xlink:href="fldm-20200930.xsd#ConvertibleNotesandCreditFacilityTables"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/ConvertibleNotesandCreditFacilityTables" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/ConvertibleNotesandCreditFacilityNarrativeDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#ConvertibleNotesandCreditFacilityNarrativeDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/ConvertibleNotesandCreditFacilityNarrativeDetails" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/ConvertibleNotesandCreditFacilityScheduleofDebtDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#ConvertibleNotesandCreditFacilityScheduleofDebtDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/ConvertibleNotesandCreditFacilityScheduleofDebtDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_ee1a38cf-3a60-4d3a-ab29-a43ccc00b6a6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongTermDebt"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentCarryingAmount_8656cb69-5256-4620-96ae-539cf4b8218d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentCarryingAmount"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LongTermDebt_ee1a38cf-3a60-4d3a-ab29-a43ccc00b6a6" xlink:to="loc_us-gaap_DebtInstrumentCarryingAmount_8656cb69-5256-4620-96ae-539cf4b8218d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentUnamortizedDiscount_33f2c3bf-d254-43a3-bd99-71b43e779277" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DebtInstrumentUnamortizedDiscount"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LongTermDebt_ee1a38cf-3a60-4d3a-ab29-a43ccc00b6a6" xlink:to="loc_us-gaap_DebtInstrumentUnamortizedDiscount_33f2c3bf-d254-43a3-bd99-71b43e779277" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredFinanceCostsNet_17c9bec2-ad1b-45af-b498-5710e711f692" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_DeferredFinanceCostsNet"/>
    <link:calculationArc order="3" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LongTermDebt_ee1a38cf-3a60-4d3a-ab29-a43ccc00b6a6" xlink:to="loc_us-gaap_DeferredFinanceCostsNet_17c9bec2-ad1b-45af-b498-5710e711f692" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://fluidigm.com/role/Leases" xlink:type="simple" xlink:href="fldm-20200930.xsd#Leases"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/Leases" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/LeasesTables" xlink:type="simple" xlink:href="fldm-20200930.xsd#LeasesTables"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/LeasesTables" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/LeasesNarrativeDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#LeasesNarrativeDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/LeasesNarrativeDetails" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/LeasesSupplementalBalancesheetDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#LeasesSupplementalBalancesheetDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/LeasesSupplementalBalancesheetDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiability_7c37f4cb-1a3a-4123-b6d4-51f7dbaf503d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingLeaseLiability"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityCurrent_3c3d4a2c-a516-403e-9058-3ca181acb108" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingLeaseLiabilityCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingLeaseLiability_7c37f4cb-1a3a-4123-b6d4-51f7dbaf503d" xlink:to="loc_us-gaap_OperatingLeaseLiabilityCurrent_3c3d4a2c-a516-403e-9058-3ca181acb108" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_e1808a54-6804-427a-b7be-9c2c687a8afa" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingLeaseLiabilityNoncurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingLeaseLiability_7c37f4cb-1a3a-4123-b6d4-51f7dbaf503d" xlink:to="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_e1808a54-6804-427a-b7be-9c2c687a8afa" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseRightOfUseAsset_463781c5-73e5-4d3e-b48c-b7216f0878b6" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingLeaseRightOfUseAsset"/>
    <link:loc xlink:type="locator" xlink:label="loc_fldm_OperatingLeaseRightOfUseAssetGross_74b45600-2a0a-4c2d-b30f-14fe9bd600a4" xlink:href="fldm-20200930.xsd#fldm_OperatingLeaseRightOfUseAssetGross"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingLeaseRightOfUseAsset_463781c5-73e5-4d3e-b48c-b7216f0878b6" xlink:to="loc_fldm_OperatingLeaseRightOfUseAssetGross_74b45600-2a0a-4c2d-b30f-14fe9bd600a4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_fldm_OperatingLeaseRightOfUseAssetAccumulatedAmortization_8843f4f4-be07-4a13-a3b9-2fbb55615239" xlink:href="fldm-20200930.xsd#fldm_OperatingLeaseRightOfUseAssetAccumulatedAmortization"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingLeaseRightOfUseAsset_463781c5-73e5-4d3e-b48c-b7216f0878b6" xlink:to="loc_fldm_OperatingLeaseRightOfUseAssetAccumulatedAmortization_8843f4f4-be07-4a13-a3b9-2fbb55615239" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://fluidigm.com/role/LeasesScheduleofOperatingLeaseCostDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#LeasesScheduleofOperatingLeaseCostDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/LeasesScheduleofOperatingLeaseCostDetails" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/LeasesOperatingLeaseMaturityDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#LeasesOperatingLeaseMaturityDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/LeasesOperatingLeaseMaturityDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_68e2e585-c1d5-47f7-87c9-a317b0ea4634" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo_e8aa9d9f-cf1b-42de-8a26-9b83bf817861" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_68e2e585-c1d5-47f7-87c9-a317b0ea4634" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo_e8aa9d9f-cf1b-42de-8a26-9b83bf817861" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree_f29aee12-92d1-4fe9-be44-1eaf42efd560" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_68e2e585-c1d5-47f7-87c9-a317b0ea4634" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree_f29aee12-92d1-4fe9-be44-1eaf42efd560" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour_c2a6a2d5-9393-44b0-8aef-1ac5ed042a7b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_68e2e585-c1d5-47f7-87c9-a317b0ea4634" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour_c2a6a2d5-9393-44b0-8aef-1ac5ed042a7b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_fldm_LesseeOperatingLeaseLiabilityToBePaidAfterYearFour_91cf71a7-73a1-47d0-b24a-b826fa7a7557" xlink:href="fldm-20200930.xsd#fldm_LesseeOperatingLeaseLiabilityToBePaidAfterYearFour"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_68e2e585-c1d5-47f7-87c9-a317b0ea4634" xlink:to="loc_fldm_LesseeOperatingLeaseLiabilityToBePaidAfterYearFour_91cf71a7-73a1-47d0-b24a-b826fa7a7557" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths_50ebe363-6c58-403e-a9f9-6ccaaa6f24ee" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_68e2e585-c1d5-47f7-87c9-a317b0ea4634" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths_50ebe363-6c58-403e-a9f9-6ccaaa6f24ee" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear_70d64fe5-b83d-477d-b129-e7af27be3837" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_68e2e585-c1d5-47f7-87c9-a317b0ea4634" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear_70d64fe5-b83d-477d-b129-e7af27be3837" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://fluidigm.com/role/LeasesOperatingLeaseMaturityDetails_1" xlink:type="simple" xlink:href="fldm-20200930.xsd#LeasesOperatingLeaseMaturityDetails_1"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/LeasesOperatingLeaseMaturityDetails_1" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_fa4293e5-fd84-4661-8809-d20002741cc2" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount_09ccc4de-1363-4366-b7fe-76e396f10608" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_fa4293e5-fd84-4661-8809-d20002741cc2" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount_09ccc4de-1363-4366-b7fe-76e396f10608" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiability_fbdb2949-1ce5-450f-999e-ce95f6bec60b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_OperatingLeaseLiability"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_fa4293e5-fd84-4661-8809-d20002741cc2" xlink:to="loc_us-gaap_OperatingLeaseLiability_fbdb2949-1ce5-450f-999e-ce95f6bec60b" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://fluidigm.com/role/FairValueofFinancialInstruments" xlink:type="simple" xlink:href="fldm-20200930.xsd#FairValueofFinancialInstruments"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/FairValueofFinancialInstruments" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/FairValueofFinancialInstrumentsTables" xlink:type="simple" xlink:href="fldm-20200930.xsd#FairValueofFinancialInstrumentsTables"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/FairValueofFinancialInstrumentsTables" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/FairValueofFinancialInstrumentsSummaryofInvestmentsandCashEquivalentsDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#FairValueofFinancialInstrumentsSummaryofInvestmentsandCashEquivalentsDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/FairValueofFinancialInstrumentsSummaryofInvestmentsandCashEquivalentsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_d56be788-13aa-44a5-a7f7-f4b4b16b5120" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AvailableForSaleSecuritiesDebtSecurities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_06e7e0e7-5bd5-423c-959b-b00b89e4f849" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_d56be788-13aa-44a5-a7f7-f4b4b16b5120" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_06e7e0e7-5bd5-423c-959b-b00b89e4f849" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShortTermInvestments_e711591f-061b-41b6-a6b7-11d27480c8be" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShortTermInvestments"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_d56be788-13aa-44a5-a7f7-f4b4b16b5120" xlink:to="loc_us-gaap_ShortTermInvestments_e711591f-061b-41b6-a6b7-11d27480c8be" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermInvestments_0b6b6d50-7a8a-42a1-b41f-778e1d9cafcc" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_LongTermInvestments"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_d56be788-13aa-44a5-a7f7-f4b4b16b5120" xlink:to="loc_us-gaap_LongTermInvestments_0b6b6d50-7a8a-42a1-b41f-778e1d9cafcc" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_fldm_CashCashEquivalentsAndShortTermInvestmentsAmortizedCost_7c0035c5-8ed4-4654-98be-902ee580201b" xlink:href="fldm-20200930.xsd#fldm_CashCashEquivalentsAndShortTermInvestmentsAmortizedCost"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax_dc44886c-5e08-4e27-9180-81b512c9ce5b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_fldm_CashCashEquivalentsAndShortTermInvestmentsAmortizedCost_7c0035c5-8ed4-4654-98be-902ee580201b" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax_dc44886c-5e08-4e27-9180-81b512c9ce5b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax_441c8f5d-31cf-408c-aa92-ba48c8f29f92" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_fldm_CashCashEquivalentsAndShortTermInvestmentsAmortizedCost_7c0035c5-8ed4-4654-98be-902ee580201b" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax_441c8f5d-31cf-408c-aa92-ba48c8f29f92" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsAndShortTermInvestments_ed81ddf9-7312-4e76-bf45-a2dccf689d6f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CashCashEquivalentsAndShortTermInvestments"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_fldm_CashCashEquivalentsAndShortTermInvestmentsAmortizedCost_7c0035c5-8ed4-4654-98be-902ee580201b" xlink:to="loc_us-gaap_CashCashEquivalentsAndShortTermInvestments_ed81ddf9-7312-4e76-bf45-a2dccf689d6f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis_ab9f44b3-9fb1-48ad-bd58-83c860d5ba0f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax_469bd4c5-15c5-4fa4-b311-64a9c7b36a46" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis_ab9f44b3-9fb1-48ad-bd58-83c860d5ba0f" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax_469bd4c5-15c5-4fa4-b311-64a9c7b36a46" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax_994dc1e9-7779-46cd-aae2-4fdc6841e77f" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis_ab9f44b3-9fb1-48ad-bd58-83c860d5ba0f" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax_994dc1e9-7779-46cd-aae2-4fdc6841e77f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_bf023938-a8b5-4bf3-91a1-205c444f0cc3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_AvailableForSaleSecuritiesDebtSecurities"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis_ab9f44b3-9fb1-48ad-bd58-83c860d5ba0f" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_bf023938-a8b5-4bf3-91a1-205c444f0cc3" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_84595993-8467-410a-8483-7049b510c460" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_efe8d85e-c8bc-4d9e-8224-b602c3dc69c8" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_84595993-8467-410a-8483-7049b510c460" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_efe8d85e-c8bc-4d9e-8224-b602c3dc69c8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashAndCashEquivalents_2ca94277-79d8-4caf-8a05-493080cab1b3" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestrictedCashAndCashEquivalents"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_84595993-8467-410a-8483-7049b510c460" xlink:to="loc_us-gaap_RestrictedCashAndCashEquivalents_2ca94277-79d8-4caf-8a05-493080cab1b3" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsAndShortTermInvestments_db0febf6-20eb-43e7-be71-d3bbd2760cfb" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CashCashEquivalentsAndShortTermInvestments"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_08c36297-5ffc-44ff-8f03-393e23589307" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsAndShortTermInvestments_db0febf6-20eb-43e7-be71-d3bbd2760cfb" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_08c36297-5ffc-44ff-8f03-393e23589307" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShortTermInvestments_2d3d3ed3-c594-4bbc-b00a-40c6118ef32b" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_ShortTermInvestments"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsAndShortTermInvestments_db0febf6-20eb-43e7-be71-d3bbd2760cfb" xlink:to="loc_us-gaap_ShortTermInvestments_2d3d3ed3-c594-4bbc-b00a-40c6118ef32b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashAndCashEquivalents_9af306d0-770e-4acc-89e7-ca235c72b30d" xlink:href="http://xbrl.fasb.org/us-gaap/2020/elts/us-gaap-2020-01-31.xsd#us-gaap_RestrictedCashAndCashEquivalents"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsAndShortTermInvestments_db0febf6-20eb-43e7-be71-d3bbd2760cfb" xlink:to="loc_us-gaap_RestrictedCashAndCashEquivalents_9af306d0-770e-4acc-89e7-ca235c72b30d" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://fluidigm.com/role/FairValueofFinancialInstrumentsScheduleofDebtDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#FairValueofFinancialInstrumentsScheduleofDebtDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/FairValueofFinancialInstrumentsScheduleofDebtDetails" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/ShareholdersEquity" xlink:type="simple" xlink:href="fldm-20200930.xsd#ShareholdersEquity"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/ShareholdersEquity" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/ShareholdersEquityTables" xlink:type="simple" xlink:href="fldm-20200930.xsd#ShareholdersEquityTables"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/ShareholdersEquityTables" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/ShareholdersEquityNarrativeDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#ShareholdersEquityNarrativeDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/ShareholdersEquityNarrativeDetails" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/ShareholdersEquityScheduleofStockOptionsDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#ShareholdersEquityScheduleofStockOptionsDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/ShareholdersEquityScheduleofStockOptionsDetails" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/StockBasedPlans" xlink:type="simple" xlink:href="fldm-20200930.xsd#StockBasedPlans"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/StockBasedPlans" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/StockBasedPlansTables" xlink:type="simple" xlink:href="fldm-20200930.xsd#StockBasedPlansTables"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/StockBasedPlansTables" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/StockBasedPlansNarrativeDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#StockBasedPlansNarrativeDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/StockBasedPlansNarrativeDetails" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/StockBasedPlansRestrictedandPerformanceStockUnitsDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#StockBasedPlansRestrictedandPerformanceStockUnitsDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/StockBasedPlansRestrictedandPerformanceStockUnitsDetails" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/StockBasedPlansStockOptionActivityDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#StockBasedPlansStockOptionActivityDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/StockBasedPlansStockOptionActivityDetails" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/StockBasedPlansStockBasedCompensationExpenseDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#StockBasedPlansStockBasedCompensationExpenseDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/StockBasedPlansStockBasedCompensationExpenseDetails" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/IncomeTaxes" xlink:type="simple" xlink:href="fldm-20200930.xsd#IncomeTaxes"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/IncomeTaxes" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/IncomeTaxesDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#IncomeTaxesDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/IncomeTaxesDetails" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/InformationAboutGeographicAreas" xlink:type="simple" xlink:href="fldm-20200930.xsd#InformationAboutGeographicAreas"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/InformationAboutGeographicAreas" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/InformationAboutGeographicAreasDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#InformationAboutGeographicAreasDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/InformationAboutGeographicAreasDetails" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/DevelopmentAgreement" xlink:type="simple" xlink:href="fldm-20200930.xsd#DevelopmentAgreement"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/DevelopmentAgreement" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/DevelopmentAgreementDetails" xlink:type="simple" xlink:href="fldm-20200930.xsd#DevelopmentAgreementDetails"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/DevelopmentAgreementDetails" xlink:type="extended"/>
  <link:roleRef roleURI="http://fluidigm.com/role/CommitmentsandContingencies" xlink:type="simple" xlink:href="fldm-20200930.xsd#CommitmentsandContingencies"/>
  <link:calculationLink xlink:role="http://fluidigm.com/role/CommitmentsandContingencies" xlink:type="extended"/>
</link:linkbase>
