<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2024 Workiva-->
<!--r:b9ae9650-ee7f-4845-8e5f-30888f6c024a,g:8d716185-87fd-40e1-9d0e-5f2253c87fbb-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://www.gladstoneland.com/role/Cover" xlink:type="simple" xlink:href="land-20240630.xsd#Cover"/>
  <link:definitionLink xlink:role="http://www.gladstoneland.com/role/Cover" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntitiesTable_0c014e5b-52bf-45cc-99ea-595b7ceb9680" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntitiesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_EntitiesTable_0c014e5b-52bf-45cc-99ea-595b7ceb9680" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_8142383a-0249-4dfd-956c-a1f411f221c3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_dei_EntitiesTable_0c014e5b-52bf-45cc-99ea-595b7ceb9680" xlink:to="loc_us-gaap_StatementClassOfStockAxis_8142383a-0249-4dfd-956c-a1f411f221c3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_8142383a-0249-4dfd-956c-a1f411f221c3_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_8142383a-0249-4dfd-956c-a1f411f221c3" xlink:to="loc_us-gaap_ClassOfStockDomain_8142383a-0249-4dfd-956c-a1f411f221c3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_08ccec30-b710-4e27-b145-be146f7374c8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_8142383a-0249-4dfd-956c-a1f411f221c3" xlink:to="loc_us-gaap_ClassOfStockDomain_08ccec30-b710-4e27-b145-be146f7374c8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_b43dcde5-a7b1-4b81-b7b7-30f91ecc7d17" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_08ccec30-b710-4e27-b145-be146f7374c8" xlink:to="loc_us-gaap_CommonStockMember_b43dcde5-a7b1-4b81-b7b7-30f91ecc7d17" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesBPreferredStockMember_fe62dacd-189a-48fc-a92f-94183ed35f23" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesBPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_08ccec30-b710-4e27-b145-be146f7374c8" xlink:to="loc_us-gaap_SeriesBPreferredStockMember_fe62dacd-189a-48fc-a92f-94183ed35f23" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesCPreferredStockMember_af048732-128a-4d59-b7a2-5382aea8dd75" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesCPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_08ccec30-b710-4e27-b145-be146f7374c8" xlink:to="loc_us-gaap_SeriesCPreferredStockMember_af048732-128a-4d59-b7a2-5382aea8dd75" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesDPreferredStockMember_073f19b2-cd34-4575-a83c-fdec41b44d51" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesDPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_08ccec30-b710-4e27-b145-be146f7374c8" xlink:to="loc_us-gaap_SeriesDPreferredStockMember_073f19b2-cd34-4575-a83c-fdec41b44d51" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentType_37bf3485-1a49-443b-a6de-a8c575ba0406" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentType"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_DocumentType_37bf3485-1a49-443b-a6de-a8c575ba0406" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentQuarterlyReport_22a6a94a-5816-4efe-bbf1-f8c8677093b3" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentQuarterlyReport"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_DocumentQuarterlyReport_22a6a94a-5816-4efe-bbf1-f8c8677093b3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentPeriodEndDate_9b4dd4bf-2d67-4643-8f7a-c0d3cf368723" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentPeriodEndDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_DocumentPeriodEndDate_9b4dd4bf-2d67-4643-8f7a-c0d3cf368723" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentTransitionReport_2309f2a7-7178-4e3b-9cd2-eb78d1e758ae" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentTransitionReport"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_DocumentTransitionReport_2309f2a7-7178-4e3b-9cd2-eb78d1e758ae" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFileNumber_49868709-43da-4b34-8ae2-383b5020870a" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityFileNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_EntityFileNumber_49868709-43da-4b34-8ae2-383b5020870a" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityRegistrantName_f9fef55e-2ec2-40c6-bdc5-59a14c202fab" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityRegistrantName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_EntityRegistrantName_f9fef55e-2ec2-40c6-bdc5-59a14c202fab" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityIncorporationStateCountryCode_16f782c0-21cc-4791-a3c5-f29deae502b7" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityIncorporationStateCountryCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_EntityIncorporationStateCountryCode_16f782c0-21cc-4791-a3c5-f29deae502b7" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityTaxIdentificationNumber_b6d888c7-8ad0-40d2-b3b2-34ad1240f9d6" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityTaxIdentificationNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_EntityTaxIdentificationNumber_b6d888c7-8ad0-40d2-b3b2-34ad1240f9d6" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressAddressLine1_518de0bd-132e-47e2-9b06-dc43e4f43452" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressAddressLine1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_EntityAddressAddressLine1_518de0bd-132e-47e2-9b06-dc43e4f43452" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressAddressLine2_c8d09d5b-923d-4ea4-9571-ce189ab067f1" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressAddressLine2"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_EntityAddressAddressLine2_c8d09d5b-923d-4ea4-9571-ce189ab067f1" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressCityOrTown_707ed421-e70b-4039-b8c5-00549e115dea" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressCityOrTown"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_EntityAddressCityOrTown_707ed421-e70b-4039-b8c5-00549e115dea" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressStateOrProvince_e07d55a6-fc23-4022-8438-321f4efba133" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressStateOrProvince"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_EntityAddressStateOrProvince_e07d55a6-fc23-4022-8438-321f4efba133" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressPostalZipCode_d5184175-b0db-4d7c-8615-cec726682c03" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressPostalZipCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_EntityAddressPostalZipCode_d5184175-b0db-4d7c-8615-cec726682c03" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CityAreaCode_d0f70f02-83c2-4a73-a6de-6faac15c3828" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_CityAreaCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_CityAreaCode_d0f70f02-83c2-4a73-a6de-6faac15c3828" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LocalPhoneNumber_9ce0415d-ad3b-44aa-a0d4-1e3abb649968" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_LocalPhoneNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_LocalPhoneNumber_9ce0415d-ad3b-44aa-a0d4-1e3abb649968" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_Security12bTitle_0ad6dd32-265a-4ea6-89ab-0b9565cd971f" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_Security12bTitle"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_Security12bTitle_0ad6dd32-265a-4ea6-89ab-0b9565cd971f" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_TradingSymbol_284dc4fe-b593-43f0-b75d-28581c13c783" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_TradingSymbol"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_TradingSymbol_284dc4fe-b593-43f0-b75d-28581c13c783" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_SecurityExchangeName_7e6d0d1e-67f6-4851-a7f3-f9a7f298a2fb" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_SecurityExchangeName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_SecurityExchangeName_7e6d0d1e-67f6-4851-a7f3-f9a7f298a2fb" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCurrentReportingStatus_43f1548f-7cd0-4364-a996-bcc2b2a48b2c" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityCurrentReportingStatus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_EntityCurrentReportingStatus_43f1548f-7cd0-4364-a996-bcc2b2a48b2c" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityInteractiveDataCurrent_e2b0672e-2686-42c7-89aa-3b631eb2170d" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityInteractiveDataCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_EntityInteractiveDataCurrent_e2b0672e-2686-42c7-89aa-3b631eb2170d" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFilerCategory_6715af44-e7d9-4cd6-8467-934c178c9ba1" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityFilerCategory"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_EntityFilerCategory_6715af44-e7d9-4cd6-8467-934c178c9ba1" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntitySmallBusiness_88b73d66-6584-487d-9e83-75eedf30c312" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntitySmallBusiness"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_EntitySmallBusiness_88b73d66-6584-487d-9e83-75eedf30c312" xlink:type="arc" order="21"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityEmergingGrowthCompany_b42904d3-729d-4b90-b1cd-3ec9c0763049" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityEmergingGrowthCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_EntityEmergingGrowthCompany_b42904d3-729d-4b90-b1cd-3ec9c0763049" xlink:type="arc" order="22"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityShellCompany_591f2cfa-577f-40e9-8999-f196a0bf55d9" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityShellCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_EntityShellCompany_591f2cfa-577f-40e9-8999-f196a0bf55d9" xlink:type="arc" order="23"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCommonStockSharesOutstanding_14a9dc89-e49d-4610-b33f-ffe7e204dc1d" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityCommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_EntityCommonStockSharesOutstanding_14a9dc89-e49d-4610-b33f-ffe7e204dc1d" xlink:type="arc" order="24"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCentralIndexKey_b7f70893-0acf-43fd-8b9c-c48ab80f372b" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityCentralIndexKey"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_EntityCentralIndexKey_b7f70893-0acf-43fd-8b9c-c48ab80f372b" xlink:type="arc" order="25"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentFiscalYearFocus_e400021a-b3a4-483d-b420-6e978748e7c4" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentFiscalYearFocus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_DocumentFiscalYearFocus_e400021a-b3a4-483d-b420-6e978748e7c4" xlink:type="arc" order="26"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentFiscalPeriodFocus_b89d52f7-9cfa-448b-956f-62d94e8d4c41" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentFiscalPeriodFocus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_DocumentFiscalPeriodFocus_b89d52f7-9cfa-448b-956f-62d94e8d4c41" xlink:type="arc" order="27"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CurrentFiscalYearEndDate_c41efaec-a854-43dd-a282-fc841c950624" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_CurrentFiscalYearEndDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_CurrentFiscalYearEndDate_c41efaec-a854-43dd-a282-fc841c950624" xlink:type="arc" order="28"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_AmendmentFlag_8597afcb-0779-4c57-8f3a-74808f7afa88" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_AmendmentFlag"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityInformationLineItems_41682a83-4b82-4179-b248-01b1d8396fb1" xlink:to="loc_dei_AmendmentFlag_8597afcb-0779-4c57-8f3a-74808f7afa88" xlink:type="arc" order="29"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.gladstoneland.com/role/CondensedConsolidatedBalanceSheets" xlink:type="simple" xlink:href="land-20240630.xsd#CondensedConsolidatedBalanceSheets"/>
  <link:definitionLink xlink:role="http://www.gladstoneland.com/role/CondensedConsolidatedBalanceSheets" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_72684a0b-84f6-4673-8f9e-5046668ab210" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_b33b8054-432c-43ae-b80d-6ba54e9bb136" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_72684a0b-84f6-4673-8f9e-5046668ab210" xlink:to="loc_us-gaap_StatementTable_b33b8054-432c-43ae-b80d-6ba54e9bb136" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_83a2e752-69fb-420b-b2f1-acde3a163f33" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_b33b8054-432c-43ae-b80d-6ba54e9bb136" xlink:to="loc_us-gaap_StatementClassOfStockAxis_83a2e752-69fb-420b-b2f1-acde3a163f33" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_83a2e752-69fb-420b-b2f1-acde3a163f33_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_83a2e752-69fb-420b-b2f1-acde3a163f33" xlink:to="loc_us-gaap_ClassOfStockDomain_83a2e752-69fb-420b-b2f1-acde3a163f33_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_1a1b1711-5125-441b-9781-a3695a153d80" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_83a2e752-69fb-420b-b2f1-acde3a163f33" xlink:to="loc_us-gaap_ClassOfStockDomain_1a1b1711-5125-441b-9781-a3695a153d80" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesDPreferredStockMember_4d369523-a307-48bd-9863-9794a70efdf4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesDPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_1a1b1711-5125-441b-9781-a3695a153d80" xlink:to="loc_us-gaap_SeriesDPreferredStockMember_4d369523-a307-48bd-9863-9794a70efdf4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesBPreferredStockMember_2078763e-7397-4632-a80f-69116b88151b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesBPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_1a1b1711-5125-441b-9781-a3695a153d80" xlink:to="loc_us-gaap_SeriesBPreferredStockMember_2078763e-7397-4632-a80f-69116b88151b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesCPreferredStockMember_fc716bfa-8c95-43cd-b864-a638b233c779" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesCPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_1a1b1711-5125-441b-9781-a3695a153d80" xlink:to="loc_us-gaap_SeriesCPreferredStockMember_fc716bfa-8c95-43cd-b864-a638b233c779" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesEPreferredStockMember_a76b21a7-5029-4e4c-84f1-1a6c100c0354" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesEPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_1a1b1711-5125-441b-9781-a3695a153d80" xlink:to="loc_us-gaap_SeriesEPreferredStockMember_a76b21a7-5029-4e4c-84f1-1a6c100c0354" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_02af87f4-aeb8-4d85-bc16-e791b3da5f5d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyTransactionsByRelatedPartyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_b33b8054-432c-43ae-b80d-6ba54e9bb136" xlink:to="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_02af87f4-aeb8-4d85-bc16-e791b3da5f5d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_02af87f4-aeb8-4d85-bc16-e791b3da5f5d_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_02af87f4-aeb8-4d85-bc16-e791b3da5f5d" xlink:to="loc_us-gaap_RelatedPartyDomain_02af87f4-aeb8-4d85-bc16-e791b3da5f5d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_d3291731-f4f4-423a-b74a-8fb69ee919ff" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_02af87f4-aeb8-4d85-bc16-e791b3da5f5d" xlink:to="loc_us-gaap_RelatedPartyDomain_d3291731-f4f4-423a-b74a-8fb69ee919ff" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyMember_51440427-36cb-4b92-bd25-58fbbd25fb43" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyDomain_d3291731-f4f4-423a-b74a-8fb69ee919ff" xlink:to="loc_us-gaap_RelatedPartyMember_51440427-36cb-4b92-bd25-58fbbd25fb43" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonrelatedPartyMember_e008b603-2f0a-4029-a687-5168cae188fd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NonrelatedPartyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyDomain_d3291731-f4f4-423a-b74a-8fb69ee919ff" xlink:to="loc_us-gaap_NonrelatedPartyMember_e008b603-2f0a-4029-a687-5168cae188fd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsAbstract_06940faf-5569-43b8-bf52-207c1a9b449b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AssetsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_72684a0b-84f6-4673-8f9e-5046668ab210" xlink:to="loc_us-gaap_AssetsAbstract_06940faf-5569-43b8-bf52-207c1a9b449b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RealEstateInvestmentPropertyAtCost_ddda9950-f131-4dcd-92d8-73e59d916bb5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RealEstateInvestmentPropertyAtCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_06940faf-5569-43b8-bf52-207c1a9b449b" xlink:to="loc_us-gaap_RealEstateInvestmentPropertyAtCost_ddda9950-f131-4dcd-92d8-73e59d916bb5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RealEstateInvestmentPropertyAccumulatedDepreciation_63e1ad7a-87fd-466e-a0eb-f161bbd646f1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RealEstateInvestmentPropertyAccumulatedDepreciation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_06940faf-5569-43b8-bf52-207c1a9b449b" xlink:to="loc_us-gaap_RealEstateInvestmentPropertyAccumulatedDepreciation_63e1ad7a-87fd-466e-a0eb-f161bbd646f1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RealEstateInvestmentPropertyNet_8a33034b-a97c-4b14-a443-23d71523f547" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RealEstateInvestmentPropertyNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_06940faf-5569-43b8-bf52-207c1a9b449b" xlink:to="loc_us-gaap_RealEstateInvestmentPropertyNet_8a33034b-a97c-4b14-a443-23d71523f547" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsNet_1b00b22e-a0da-4fc5-adcc-ba8fb7af1272" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_06940faf-5569-43b8-bf52-207c1a9b449b" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsNet_1b00b22e-a0da-4fc5-adcc-ba8fb7af1272" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RealEstateHeldforsale_981297ac-630d-4041-9903-6ffb1fe79a94" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RealEstateHeldforsale"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_06940faf-5569-43b8-bf52-207c1a9b449b" xlink:to="loc_us-gaap_RealEstateHeldforsale_981297ac-630d-4041-9903-6ffb1fe79a94" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_5dd8592c-7cd4-4dd7-bb92-24ed4bb5a5c2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_06940faf-5569-43b8-bf52-207c1a9b449b" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_5dd8592c-7cd4-4dd7-bb92-24ed4bb5a5c2" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssets_cc3a9a90-7686-42eb-b8d5-83b9f32a256a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_06940faf-5569-43b8-bf52-207c1a9b449b" xlink:to="loc_us-gaap_OtherAssets_cc3a9a90-7686-42eb-b8d5-83b9f32a256a" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Assets_c239d88b-51b6-4d09-928d-0eb1c3507b93" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Assets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract_06940faf-5569-43b8-bf52-207c1a9b449b" xlink:to="loc_us-gaap_Assets_c239d88b-51b6-4d09-928d-0eb1c3507b93" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_8c44305a-195c-44a8-9364-66a21445502c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LiabilitiesAndStockholdersEquityAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_72684a0b-84f6-4673-8f9e-5046668ab210" xlink:to="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_8c44305a-195c-44a8-9364-66a21445502c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesAbstract_636dc7b2-2b10-4a95-8fe1-36e9b45fada1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LiabilitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_8c44305a-195c-44a8-9364-66a21445502c" xlink:to="loc_us-gaap_LiabilitiesAbstract_636dc7b2-2b10-4a95-8fe1-36e9b45fada1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCredit_ad5787ed-3d70-4d38-a67f-82bedad87684" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LineOfCredit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAbstract_636dc7b2-2b10-4a95-8fe1-36e9b45fada1" xlink:to="loc_us-gaap_LineOfCredit_ad5787ed-3d70-4d38-a67f-82bedad87684" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuredLongTermDebt_85ce2f7c-ed9a-495a-8571-faa85c01efdd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SecuredLongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAbstract_636dc7b2-2b10-4a95-8fe1-36e9b45fada1" xlink:to="loc_us-gaap_SecuredLongTermDebt_85ce2f7c-ed9a-495a-8571-faa85c01efdd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharesSubjectToMandatoryRedemptionSettlementTermsAmount_b705d86e-b2ec-443d-98bc-34bc70485587" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SharesSubjectToMandatoryRedemptionSettlementTermsAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAbstract_636dc7b2-2b10-4a95-8fe1-36e9b45fada1" xlink:to="loc_us-gaap_SharesSubjectToMandatoryRedemptionSettlementTermsAmount_b705d86e-b2ec-443d-98bc-34bc70485587" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsPayableAndAccruedLiabilitiesCurrentAndNoncurrent_e99e86cf-4bc2-469c-be68-6b1c888771b8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccountsPayableAndAccruedLiabilitiesCurrentAndNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAbstract_636dc7b2-2b10-4a95-8fe1-36e9b45fada1" xlink:to="loc_us-gaap_AccountsPayableAndAccruedLiabilitiesCurrentAndNoncurrent_e99e86cf-4bc2-469c-be68-6b1c888771b8" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilities_06c01acd-e977-4980-9bd1-0b6e3452d6da" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAbstract_636dc7b2-2b10-4a95-8fe1-36e9b45fada1" xlink:to="loc_us-gaap_OtherLiabilities_06c01acd-e977-4980-9bd1-0b6e3452d6da" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_8b9c3b47-9bde-40d5-be6d-b036af2bbd78" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Liabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAbstract_636dc7b2-2b10-4a95-8fe1-36e9b45fada1" xlink:to="loc_us-gaap_Liabilities_8b9c3b47-9bde-40d5-be6d-b036af2bbd78" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommitmentsAndContingencies_23156777-44df-46bb-be28-f0eb5d54eaca" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommitmentsAndContingencies"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_8c44305a-195c-44a8-9364-66a21445502c" xlink:to="loc_us-gaap_CommitmentsAndContingencies_23156777-44df-46bb-be28-f0eb5d54eaca" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityAbstract_224dfc66-609a-4904-b5ad-b3b6490ccfae" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockholdersEquityAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_8c44305a-195c-44a8-9364-66a21445502c" xlink:to="loc_us-gaap_StockholdersEquityAbstract_224dfc66-609a-4904-b5ad-b3b6490ccfae" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockValue_1ff19a51-6f2e-499d-945d-cbb156b5ebdb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_224dfc66-609a-4904-b5ad-b3b6490ccfae" xlink:to="loc_us-gaap_PreferredStockValue_1ff19a51-6f2e-499d-945d-cbb156b5ebdb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockValue_ac111f70-e316-4fb4-b62b-64ab640a5b8d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_224dfc66-609a-4904-b5ad-b3b6490ccfae" xlink:to="loc_us-gaap_CommonStockValue_ac111f70-e316-4fb4-b62b-64ab640a5b8d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalCommonStock_585f47b3-c21f-4a09-b132-cccac4ecf4b2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AdditionalPaidInCapitalCommonStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_224dfc66-609a-4904-b5ad-b3b6490ccfae" xlink:to="loc_us-gaap_AdditionalPaidInCapitalCommonStock_585f47b3-c21f-4a09-b132-cccac4ecf4b2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDistributionsInExcessOfNetIncome_bc3fcf33-5044-463f-b0ef-7f2c92129e42" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedDistributionsInExcessOfNetIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_224dfc66-609a-4904-b5ad-b3b6490ccfae" xlink:to="loc_us-gaap_AccumulatedDistributionsInExcessOfNetIncome_bc3fcf33-5044-463f-b0ef-7f2c92129e42" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_0b04e365-58b8-46ab-b92b-14833ba4af8f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_224dfc66-609a-4904-b5ad-b3b6490ccfae" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_0b04e365-58b8-46ab-b92b-14833ba4af8f" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_94f5372d-0977-4fea-b44f-55aa848fa7b0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StockholdersEquityAbstract_224dfc66-609a-4904-b5ad-b3b6490ccfae" xlink:to="loc_us-gaap_StockholdersEquity_94f5372d-0977-4fea-b44f-55aa848fa7b0" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MinorityInterestInOperatingPartnerships_074c5157-4e8e-450f-a339-02f17f12b1d9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_MinorityInterestInOperatingPartnerships"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_8c44305a-195c-44a8-9364-66a21445502c" xlink:to="loc_us-gaap_MinorityInterestInOperatingPartnerships_074c5157-4e8e-450f-a339-02f17f12b1d9" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_95b0c3e6-f4b9-4f29-bc91-2bdebeb2c75b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_8c44305a-195c-44a8-9364-66a21445502c" xlink:to="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_95b0c3e6-f4b9-4f29-bc91-2bdebeb2c75b" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquity_21fcf20a-ef56-446d-b72e-74f60b9f64ae" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LiabilitiesAndStockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquityAbstract_8c44305a-195c-44a8-9364-66a21445502c" xlink:to="loc_us-gaap_LiabilitiesAndStockholdersEquity_21fcf20a-ef56-446d-b72e-74f60b9f64ae" xlink:type="arc" order="6"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.gladstoneland.com/role/CondensedConsolidatedBalanceSheetsParenthetical" xlink:type="simple" xlink:href="land-20240630.xsd#CondensedConsolidatedBalanceSheetsParenthetical"/>
  <link:definitionLink xlink:role="http://www.gladstoneland.com/role/CondensedConsolidatedBalanceSheetsParenthetical" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_f80a15bb-7602-45c6-91de-35a773a70aa5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_508b937a-b381-479e-b2d2-d2b47d30ce06" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_f80a15bb-7602-45c6-91de-35a773a70aa5" xlink:to="loc_us-gaap_StatementTable_508b937a-b381-479e-b2d2-d2b47d30ce06" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_3edb06aa-2269-48e9-841b-b041e44c75ba" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_508b937a-b381-479e-b2d2-d2b47d30ce06" xlink:to="loc_us-gaap_StatementClassOfStockAxis_3edb06aa-2269-48e9-841b-b041e44c75ba" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_3edb06aa-2269-48e9-841b-b041e44c75ba_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_3edb06aa-2269-48e9-841b-b041e44c75ba" xlink:to="loc_us-gaap_ClassOfStockDomain_3edb06aa-2269-48e9-841b-b041e44c75ba_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_ef50c8d5-116a-4a7e-8325-0609d735aa72" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_3edb06aa-2269-48e9-841b-b041e44c75ba" xlink:to="loc_us-gaap_ClassOfStockDomain_ef50c8d5-116a-4a7e-8325-0609d735aa72" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesDPreferredStockMember_ea1142d6-0611-43ca-abdb-7a0767ef7654" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesDPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_ef50c8d5-116a-4a7e-8325-0609d735aa72" xlink:to="loc_us-gaap_SeriesDPreferredStockMember_ea1142d6-0611-43ca-abdb-7a0767ef7654" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesBPreferredStockMember_24f1371c-3103-4178-bae5-9d699d624517" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesBPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_ef50c8d5-116a-4a7e-8325-0609d735aa72" xlink:to="loc_us-gaap_SeriesBPreferredStockMember_24f1371c-3103-4178-bae5-9d699d624517" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesCPreferredStockMember_6eca0d51-9cfb-4d47-9123-f2ac4a19a0d3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesCPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_ef50c8d5-116a-4a7e-8325-0609d735aa72" xlink:to="loc_us-gaap_SeriesCPreferredStockMember_6eca0d51-9cfb-4d47-9123-f2ac4a19a0d3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesEPreferredStockMember_5da02cb9-9606-4458-92b8-5d4c2aceaeca" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesEPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_ef50c8d5-116a-4a7e-8325-0609d735aa72" xlink:to="loc_us-gaap_SeriesEPreferredStockMember_5da02cb9-9606-4458-92b8-5d4c2aceaeca" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockParOrStatedValuePerShare_03584d2c-0146-44df-9714-70a26e5aae3d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockParOrStatedValuePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_f80a15bb-7602-45c6-91de-35a773a70aa5" xlink:to="loc_us-gaap_PreferredStockParOrStatedValuePerShare_03584d2c-0146-44df-9714-70a26e5aae3d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockLiquidationPreference_09f2bfa5-ce2e-4cc9-b9f1-9740cd2e84fb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockLiquidationPreference"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_f80a15bb-7602-45c6-91de-35a773a70aa5" xlink:to="loc_us-gaap_PreferredStockLiquidationPreference_09f2bfa5-ce2e-4cc9-b9f1-9740cd2e84fb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesAuthorized_eec3c4e9-0a9f-4339-9b74-8b512983d1ea" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_f80a15bb-7602-45c6-91de-35a773a70aa5" xlink:to="loc_us-gaap_PreferredStockSharesAuthorized_eec3c4e9-0a9f-4339-9b74-8b512983d1ea" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesIssued_5798d49f-2f95-450c-8be8-64ed4a74c261" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_f80a15bb-7602-45c6-91de-35a773a70aa5" xlink:to="loc_us-gaap_PreferredStockSharesIssued_5798d49f-2f95-450c-8be8-64ed4a74c261" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesOutstanding_3e32c788-0c54-47a4-8db3-ef9f5329decc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_f80a15bb-7602-45c6-91de-35a773a70aa5" xlink:to="loc_us-gaap_PreferredStockSharesOutstanding_3e32c788-0c54-47a4-8db3-ef9f5329decc" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockParOrStatedValuePerShare_ba7a1079-85d5-49e4-8ef7-927f9fd9ff27" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockParOrStatedValuePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_f80a15bb-7602-45c6-91de-35a773a70aa5" xlink:to="loc_us-gaap_CommonStockParOrStatedValuePerShare_ba7a1079-85d5-49e4-8ef7-927f9fd9ff27" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesAuthorized_6c2e466e-6216-4bbd-80f3-b2139ffaafa8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_f80a15bb-7602-45c6-91de-35a773a70aa5" xlink:to="loc_us-gaap_CommonStockSharesAuthorized_6c2e466e-6216-4bbd-80f3-b2139ffaafa8" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesIssued_14306b5a-298c-4bd0-a1bc-45ad6c8cbf8f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_f80a15bb-7602-45c6-91de-35a773a70aa5" xlink:to="loc_us-gaap_CommonStockSharesIssued_14306b5a-298c-4bd0-a1bc-45ad6c8cbf8f" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_c4c1a85b-6426-4b43-83b1-08d8321e8061" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_f80a15bb-7602-45c6-91de-35a773a70aa5" xlink:to="loc_us-gaap_CommonStockSharesOutstanding_c4c1a85b-6426-4b43-83b1-08d8321e8061" xlink:type="arc" order="8"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.gladstoneland.com/role/CondensedConsolidatedStatementsofEquity" xlink:type="simple" xlink:href="land-20240630.xsd#CondensedConsolidatedStatementsofEquity"/>
  <link:definitionLink xlink:role="http://www.gladstoneland.com/role/CondensedConsolidatedStatementsofEquity" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_0b0866b5-1305-449e-8eb6-6380c06173f1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_198988f8-85b8-4eea-8698-ba0f6580c278" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_0b0866b5-1305-449e-8eb6-6380c06173f1" xlink:to="loc_us-gaap_StatementTable_198988f8-85b8-4eea-8698-ba0f6580c278" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_0e8fce49-df56-4df7-bacf-fe4eea1fea46" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_198988f8-85b8-4eea-8698-ba0f6580c278" xlink:to="loc_us-gaap_StatementClassOfStockAxis_0e8fce49-df56-4df7-bacf-fe4eea1fea46" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_0e8fce49-df56-4df7-bacf-fe4eea1fea46_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_0e8fce49-df56-4df7-bacf-fe4eea1fea46" xlink:to="loc_us-gaap_ClassOfStockDomain_0e8fce49-df56-4df7-bacf-fe4eea1fea46_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_1fac9dd4-f013-43de-bceb-7f5cb7702f3b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_0e8fce49-df56-4df7-bacf-fe4eea1fea46" xlink:to="loc_us-gaap_ClassOfStockDomain_1fac9dd4-f013-43de-bceb-7f5cb7702f3b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesBPreferredStockMember_582a11da-07de-4998-9814-69503dccfee2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesBPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_1fac9dd4-f013-43de-bceb-7f5cb7702f3b" xlink:to="loc_us-gaap_SeriesBPreferredStockMember_582a11da-07de-4998-9814-69503dccfee2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesCPreferredStockMember_b5a54fd4-7b5a-4d05-a1c9-78fa53b07fa6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesCPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_1fac9dd4-f013-43de-bceb-7f5cb7702f3b" xlink:to="loc_us-gaap_SeriesCPreferredStockMember_b5a54fd4-7b5a-4d05-a1c9-78fa53b07fa6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesEPreferredStockMember_66682229-b585-4048-8bf3-aa5ab9064198" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesEPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_1fac9dd4-f013-43de-bceb-7f5cb7702f3b" xlink:to="loc_us-gaap_SeriesEPreferredStockMember_66682229-b585-4048-8bf3-aa5ab9064198" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_9f1a4442-d772-4d5f-a005-9cddaa29e6ce" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_198988f8-85b8-4eea-8698-ba0f6580c278" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_9f1a4442-d772-4d5f-a005-9cddaa29e6ce" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_9f1a4442-d772-4d5f-a005-9cddaa29e6ce_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_9f1a4442-d772-4d5f-a005-9cddaa29e6ce" xlink:to="loc_us-gaap_EquityComponentDomain_9f1a4442-d772-4d5f-a005-9cddaa29e6ce_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_f18e03c6-b51c-4634-a80a-dbdf50b94af9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_9f1a4442-d772-4d5f-a005-9cddaa29e6ce" xlink:to="loc_us-gaap_EquityComponentDomain_f18e03c6-b51c-4634-a80a-dbdf50b94af9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ParentMember_3be81011-3d98-4674-a34a-ee789895abba" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ParentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_f18e03c6-b51c-4634-a80a-dbdf50b94af9" xlink:to="loc_us-gaap_ParentMember_3be81011-3d98-4674-a34a-ee789895abba" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockMember_cd6c473f-9e1e-4e7b-9851-27ca2cf3973a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ParentMember_3be81011-3d98-4674-a34a-ee789895abba" xlink:to="loc_us-gaap_PreferredStockMember_cd6c473f-9e1e-4e7b-9851-27ca2cf3973a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_6fd2305a-feaa-46aa-8c0c-899a870f42ce" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ParentMember_3be81011-3d98-4674-a34a-ee789895abba" xlink:to="loc_us-gaap_CommonStockMember_6fd2305a-feaa-46aa-8c0c-899a870f42ce" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalMember_9ff885f7-9516-4586-b02b-52f18577a3e5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AdditionalPaidInCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ParentMember_3be81011-3d98-4674-a34a-ee789895abba" xlink:to="loc_us-gaap_AdditionalPaidInCapitalMember_9ff885f7-9516-4586-b02b-52f18577a3e5" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDistributionsInExcessOfNetIncomeMember_9836fb63-fe1a-40fb-a4bc-3887596794a9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedDistributionsInExcessOfNetIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ParentMember_3be81011-3d98-4674-a34a-ee789895abba" xlink:to="loc_us-gaap_AccumulatedDistributionsInExcessOfNetIncomeMember_9836fb63-fe1a-40fb-a4bc-3887596794a9" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_4a366504-7337-407c-9ff8-b705c186ff4f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ParentMember_3be81011-3d98-4674-a34a-ee789895abba" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_4a366504-7337-407c-9ff8-b705c186ff4f" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NoncontrollingInterestMember_c7eec7b5-7a15-4bb3-8459-c7a4bbb3d898" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NoncontrollingInterestMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_f18e03c6-b51c-4634-a80a-dbdf50b94af9" xlink:to="loc_us-gaap_NoncontrollingInterestMember_c7eec7b5-7a15-4bb3-8459-c7a4bbb3d898" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_36493326-033c-4c0f-94cf-05fdcb192946" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInStockholdersEquityRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_0b0866b5-1305-449e-8eb6-6380c06173f1" xlink:to="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_36493326-033c-4c0f-94cf-05fdcb192946" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesOutstanding_c70df260-d520-4dc0-a9e9-ea8b152e058f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_36493326-033c-4c0f-94cf-05fdcb192946" xlink:to="loc_us-gaap_PreferredStockSharesOutstanding_c70df260-d520-4dc0-a9e9-ea8b152e058f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_2d492511-840a-48e8-8ea9-3a2195939211" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_36493326-033c-4c0f-94cf-05fdcb192946" xlink:to="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_2d492511-840a-48e8-8ea9-3a2195939211" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_4d7f7be6-16be-4dee-8d77-b78575d4926b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_36493326-033c-4c0f-94cf-05fdcb192946" xlink:to="loc_us-gaap_CommonStockSharesOutstanding_4d7f7be6-16be-4dee-8d77-b78575d4926b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockRedeemedOrCalledDuringPeriodShares_bd2fc7be-03e7-47e8-aa15-71186e00cbc1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockRedeemedOrCalledDuringPeriodShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_36493326-033c-4c0f-94cf-05fdcb192946" xlink:to="loc_us-gaap_StockRedeemedOrCalledDuringPeriodShares_bd2fc7be-03e7-47e8-aa15-71186e00cbc1" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockRedeemedOrCalledDuringPeriodValue_6abfb98e-98ce-4997-b901-3fb97f7f2dd7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockRedeemedOrCalledDuringPeriodValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_36493326-033c-4c0f-94cf-05fdcb192946" xlink:to="loc_us-gaap_StockRedeemedOrCalledDuringPeriodValue_6abfb98e-98ce-4997-b901-3fb97f7f2dd7" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesNewIssues_3e74fb31-11ec-4095-bea2-ae52db1fd001" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockIssuedDuringPeriodSharesNewIssues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_36493326-033c-4c0f-94cf-05fdcb192946" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesNewIssues_3e74fb31-11ec-4095-bea2-ae52db1fd001" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueNewIssues_1f4f727d-ede3-4c33-8fa8-b3dff0f05a09" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockIssuedDuringPeriodValueNewIssues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_36493326-033c-4c0f-94cf-05fdcb192946" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueNewIssues_1f4f727d-ede3-4c33-8fa8-b3dff0f05a09" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProfitLoss_fd3d3baf-54be-4769-9041-99dfa2b80948" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProfitLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_36493326-033c-4c0f-94cf-05fdcb192946" xlink:to="loc_us-gaap_ProfitLoss_fd3d3baf-54be-4769-9041-99dfa2b80948" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DividendsPreferredStock_5bdb8d07-cd2e-41dd-aa71-be663c1fee3b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DividendsPreferredStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_36493326-033c-4c0f-94cf-05fdcb192946" xlink:to="loc_us-gaap_DividendsPreferredStock_5bdb8d07-cd2e-41dd-aa71-be663c1fee3b" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_StockholdersEquityDecreaseFromDistributions_bb863b84-51d4-42da-9f9c-8c29edda87fb" xlink:href="land-20240630.xsd#land_StockholdersEquityDecreaseFromDistributions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_36493326-033c-4c0f-94cf-05fdcb192946" xlink:to="loc_land_StockholdersEquityDecreaseFromDistributions_bb863b84-51d4-42da-9f9c-8c29edda87fb" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_d41c9050-220c-4e63-8b06-49163085ae01" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_36493326-033c-4c0f-94cf-05fdcb192946" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_d41c9050-220c-4e63-8b06-49163085ae01" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesOutstanding_69ead489-259d-4864-a8cf-91e24e892dcd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockSharesOutstanding"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_c9f84ce6-461a-4b8c-a274-2f61150e5382" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_7c62864e-bad5-4193-a424-b79c117581fa" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockSharesOutstanding"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.gladstoneland.com/role/BusinessandOrganizationDetails" xlink:type="simple" xlink:href="land-20240630.xsd#BusinessandOrganizationDetails"/>
  <link:definitionLink xlink:role="http://www.gladstoneland.com/role/BusinessandOrganizationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_land_OrganizationConsolidationAndPresentationOfFinancialStatementsDisclosureLineItems_e96679cd-482f-4ed7-b2d9-76df08796bec" xlink:href="land-20240630.xsd#land_OrganizationConsolidationAndPresentationOfFinancialStatementsDisclosureLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_OrganizationConsolidationAndPresentationOfFinancialStatementsDisclosureTable_e1d4c803-56b1-44bf-9c1c-e4e63a7a14b7" xlink:href="land-20240630.xsd#land_OrganizationConsolidationAndPresentationOfFinancialStatementsDisclosureTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_land_OrganizationConsolidationAndPresentationOfFinancialStatementsDisclosureLineItems_e96679cd-482f-4ed7-b2d9-76df08796bec" xlink:to="loc_land_OrganizationConsolidationAndPresentationOfFinancialStatementsDisclosureTable_e1d4c803-56b1-44bf-9c1c-e4e63a7a14b7" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_OwnershipAxis_9419c28e-22a3-44f7-b295-b4354effa5a8" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_OwnershipAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_land_OrganizationConsolidationAndPresentationOfFinancialStatementsDisclosureTable_e1d4c803-56b1-44bf-9c1c-e4e63a7a14b7" xlink:to="loc_srt_OwnershipAxis_9419c28e-22a3-44f7-b295-b4354effa5a8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_OwnershipDomain_9419c28e-22a3-44f7-b295-b4354effa5a8_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_OwnershipDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_OwnershipAxis_9419c28e-22a3-44f7-b295-b4354effa5a8" xlink:to="loc_srt_OwnershipDomain_9419c28e-22a3-44f7-b295-b4354effa5a8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_OwnershipDomain_593c52d0-22d8-4902-bd57-8643516f2f19" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_OwnershipDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_OwnershipAxis_9419c28e-22a3-44f7-b295-b4354effa5a8" xlink:to="loc_srt_OwnershipDomain_593c52d0-22d8-4902-bd57-8643516f2f19" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_OPUnitHolderMember_df33b19b-7139-47db-8a08-8a5feb34dad7" xlink:href="land-20240630.xsd#land_OPUnitHolderMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_OwnershipDomain_593c52d0-22d8-4902-bd57-8643516f2f19" xlink:to="loc_land_OPUnitHolderMember_df33b19b-7139-47db-8a08-8a5feb34dad7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_GladstoneLandAdvisersIncMember_aacd7d8a-9b01-4178-a380-f4959580218d" xlink:href="land-20240630.xsd#land_GladstoneLandAdvisersIncMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_OwnershipDomain_593c52d0-22d8-4902-bd57-8643516f2f19" xlink:to="loc_land_GladstoneLandAdvisersIncMember_aacd7d8a-9b01-4178-a380-f4959580218d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MinorityInterestOwnershipPercentageByParent_e1fb257b-1e77-4f5d-bb6c-c7010a01e07c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_MinorityInterestOwnershipPercentageByParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_land_OrganizationConsolidationAndPresentationOfFinancialStatementsDisclosureLineItems_e96679cd-482f-4ed7-b2d9-76df08796bec" xlink:to="loc_us-gaap_MinorityInterestOwnershipPercentageByParent_e1fb257b-1e77-4f5d-bb6c-c7010a01e07c" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.gladstoneland.com/role/RealEstateandIntangibleAssetsAdditionalInformationDetails" xlink:type="simple" xlink:href="land-20240630.xsd#RealEstateandIntangibleAssetsAdditionalInformationDetails"/>
  <link:definitionLink xlink:role="http://www.gladstoneland.com/role/RealEstateandIntangibleAssetsAdditionalInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RealEstatePropertiesLineItems_dd35902b-29c5-4ba2-992d-43c663a529bc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RealEstatePropertiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRealEstatePropertiesTable_cb6325db-da18-4ccb-9c09-ac4f003b38be" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfRealEstatePropertiesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_dd35902b-29c5-4ba2-992d-43c663a529bc" xlink:to="loc_us-gaap_ScheduleOfRealEstatePropertiesTable_cb6325db-da18-4ccb-9c09-ac4f003b38be" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_c6d39d19-6dee-48b7-93c1-ccb5c19c1ee2" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRealEstatePropertiesTable_cb6325db-da18-4ccb-9c09-ac4f003b38be" xlink:to="loc_srt_StatementGeographicalAxis_c6d39d19-6dee-48b7-93c1-ccb5c19c1ee2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_c6d39d19-6dee-48b7-93c1-ccb5c19c1ee2_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_c6d39d19-6dee-48b7-93c1-ccb5c19c1ee2" xlink:to="loc_srt_SegmentGeographicalDomain_c6d39d19-6dee-48b7-93c1-ccb5c19c1ee2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_c191cbd4-b433-4cd8-9dac-430cf62c0449" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_c6d39d19-6dee-48b7-93c1-ccb5c19c1ee2" xlink:to="loc_srt_SegmentGeographicalDomain_c191cbd4-b433-4cd8-9dac-430cf62c0449" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_stpr_FL_1496e8a7-f174-484b-b921-23776d6d0d7d" xlink:href="https://xbrl.sec.gov/stpr/2024/stpr-2024.xsd#stpr_FL"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_c191cbd4-b433-4cd8-9dac-430cf62c0449" xlink:to="loc_stpr_FL_1496e8a7-f174-484b-b921-23776d6d0d7d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_KernCaliforniaMember_38044d18-ef50-4e43-8cc1-4515d1b46fd8" xlink:href="land-20240630.xsd#land_KernCaliforniaMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_c191cbd4-b433-4cd8-9dac-430cf62c0449" xlink:to="loc_land_KernCaliforniaMember_38044d18-ef50-4e43-8cc1-4515d1b46fd8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_FresnoCAMember_69f9077a-8afa-439c-9e81-5658f9c1fa5b" xlink:href="land-20240630.xsd#land_FresnoCAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_c191cbd4-b433-4cd8-9dac-430cf62c0449" xlink:to="loc_land_FresnoCAMember_69f9077a-8afa-439c-9e81-5658f9c1fa5b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_stpr_CA_63cb30f0-79cb-4fec-a886-29f0e837133c" xlink:href="https://xbrl.sec.gov/stpr/2024/stpr-2024.xsd#stpr_CA"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_c191cbd4-b433-4cd8-9dac-430cf62c0449" xlink:to="loc_stpr_CA_63cb30f0-79cb-4fec-a886-29f0e837133c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_OwnershipAxis_eab7ea63-2975-41be-95e4-391a627de563" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_OwnershipAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRealEstatePropertiesTable_cb6325db-da18-4ccb-9c09-ac4f003b38be" xlink:to="loc_srt_OwnershipAxis_eab7ea63-2975-41be-95e4-391a627de563" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_OwnershipDomain_eab7ea63-2975-41be-95e4-391a627de563_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_OwnershipDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_OwnershipAxis_eab7ea63-2975-41be-95e4-391a627de563" xlink:to="loc_srt_OwnershipDomain_eab7ea63-2975-41be-95e4-391a627de563_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_OwnershipDomain_f29d6e70-90f0-4722-a9c9-67fbd5f6aa7f" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_OwnershipDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_OwnershipAxis_eab7ea63-2975-41be-95e4-391a627de563" xlink:to="loc_srt_OwnershipDomain_f29d6e70-90f0-4722-a9c9-67fbd5f6aa7f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_FresnoCaliforniaMember_d1ec4e7b-7dce-4872-a97d-828fd23987ba" xlink:href="land-20240630.xsd#land_FresnoCaliforniaMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_OwnershipDomain_f29d6e70-90f0-4722-a9c9-67fbd5f6aa7f" xlink:to="loc_land_FresnoCaliforniaMember_d1ec4e7b-7dce-4872-a97d-828fd23987ba" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_UmatillaOregonMember_88a352f3-e6cf-4d7a-a0c3-69bc66c9f7d6" xlink:href="land-20240630.xsd#land_UmatillaOregonMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_OwnershipDomain_f29d6e70-90f0-4722-a9c9-67fbd5f6aa7f" xlink:to="loc_land_UmatillaOregonMember_88a352f3-e6cf-4d7a-a0c3-69bc66c9f7d6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_FresnoCaliforniaAndUmatillaOregonMember_db02c4c3-b087-4fe3-a98c-a655d26211b7" xlink:href="land-20240630.xsd#land_FresnoCaliforniaAndUmatillaOregonMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_OwnershipDomain_f29d6e70-90f0-4722-a9c9-67fbd5f6aa7f" xlink:to="loc_land_FresnoCaliforniaAndUmatillaOregonMember_db02c4c3-b087-4fe3-a98c-a655d26211b7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MortgageLoansOnRealEstateDescriptionTypeOfPropertyAxis_a36f8cb7-4d9a-40b8-b77c-378ecf93a9d0" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_MortgageLoansOnRealEstateDescriptionTypeOfPropertyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRealEstatePropertiesTable_cb6325db-da18-4ccb-9c09-ac4f003b38be" xlink:to="loc_srt_MortgageLoansOnRealEstateDescriptionTypeOfPropertyAxis_a36f8cb7-4d9a-40b8-b77c-378ecf93a9d0" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MortgageLoansOnRealEstateNamePropertyTypeDomain_a36f8cb7-4d9a-40b8-b77c-378ecf93a9d0_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_MortgageLoansOnRealEstateNamePropertyTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_MortgageLoansOnRealEstateDescriptionTypeOfPropertyAxis_a36f8cb7-4d9a-40b8-b77c-378ecf93a9d0" xlink:to="loc_srt_MortgageLoansOnRealEstateNamePropertyTypeDomain_a36f8cb7-4d9a-40b8-b77c-378ecf93a9d0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MortgageLoansOnRealEstateNamePropertyTypeDomain_d34f8346-9c7e-4656-ac8c-ed6ba87c1512" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_MortgageLoansOnRealEstateNamePropertyTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_MortgageLoansOnRealEstateDescriptionTypeOfPropertyAxis_a36f8cb7-4d9a-40b8-b77c-378ecf93a9d0" xlink:to="loc_srt_MortgageLoansOnRealEstateNamePropertyTypeDomain_d34f8346-9c7e-4656-ac8c-ed6ba87c1512" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_NewRealEstateActivity2022Member_61d23b0c-a409-4d27-b95d-48437dffae7b" xlink:href="land-20240630.xsd#land_NewRealEstateActivity2022Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_MortgageLoansOnRealEstateNamePropertyTypeDomain_d34f8346-9c7e-4656-ac8c-ed6ba87c1512" xlink:to="loc_land_NewRealEstateActivity2022Member_61d23b0c-a409-4d27-b95d-48437dffae7b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CounterpartyNameAxis_b7732a54-a73e-48be-8d75-e74ddc898544" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_CounterpartyNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRealEstatePropertiesTable_cb6325db-da18-4ccb-9c09-ac4f003b38be" xlink:to="loc_srt_CounterpartyNameAxis_b7732a54-a73e-48be-8d75-e74ddc898544" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_b7732a54-a73e-48be-8d75-e74ddc898544_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CounterpartyNameAxis_b7732a54-a73e-48be-8d75-e74ddc898544" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_b7732a54-a73e-48be-8d75-e74ddc898544_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_7ed8fa8e-7962-4663-83a4-bb3acbcdd270" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CounterpartyNameAxis_b7732a54-a73e-48be-8d75-e74ddc898544" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_7ed8fa8e-7962-4663-83a4-bb3acbcdd270" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_WestlandsWaterDistrictMember_6536987b-1461-4973-9a22-1ea03263825a" xlink:href="land-20240630.xsd#land_WestlandsWaterDistrictMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_7ed8fa8e-7962-4663-83a4-bb3acbcdd270" xlink:to="loc_land_WestlandsWaterDistrictMember_6536987b-1461-4973-9a22-1ea03263825a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_OtherVariousWaterAgreementsMember_51b5fde8-435a-4a46-8491-d538f1f2879d" xlink:href="land-20240630.xsd#land_OtherVariousWaterAgreementsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_7ed8fa8e-7962-4663-83a4-bb3acbcdd270" xlink:to="loc_land_OtherVariousWaterAgreementsMember_51b5fde8-435a-4a46-8491-d538f1f2879d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingenciesByNatureOfContingencyAxis_99b8e429-7abb-4fbb-966d-852bccf09d39" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LossContingenciesByNatureOfContingencyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRealEstatePropertiesTable_cb6325db-da18-4ccb-9c09-ac4f003b38be" xlink:to="loc_us-gaap_LossContingenciesByNatureOfContingencyAxis_99b8e429-7abb-4fbb-966d-852bccf09d39" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingencyNatureDomain_99b8e429-7abb-4fbb-966d-852bccf09d39_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LossContingencyNatureDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LossContingenciesByNatureOfContingencyAxis_99b8e429-7abb-4fbb-966d-852bccf09d39" xlink:to="loc_us-gaap_LossContingencyNatureDomain_99b8e429-7abb-4fbb-966d-852bccf09d39_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingencyNatureDomain_b5a2a473-d0b3-4fa0-977e-117198ead2f4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LossContingencyNatureDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LossContingenciesByNatureOfContingencyAxis_99b8e429-7abb-4fbb-966d-852bccf09d39" xlink:to="loc_us-gaap_LossContingencyNatureDomain_b5a2a473-d0b3-4fa0-977e-117198ead2f4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossFromCatastrophesMember_46733b6d-c84f-4821-a1da-8ad729d67b05" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LossFromCatastrophesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingencyNatureDomain_b5a2a473-d0b3-4fa0-977e-117198ead2f4" xlink:to="loc_us-gaap_LossFromCatastrophesMember_46733b6d-c84f-4821-a1da-8ad729d67b05" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_NumberOfRealEstateFarms_9f9986dc-e1cd-4628-856c-2c10fb6e2c39" xlink:href="land-20240630.xsd#land_NumberOfRealEstateFarms"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_dd35902b-29c5-4ba2-992d-43c663a529bc" xlink:to="loc_land_NumberOfRealEstateFarms_9f9986dc-e1cd-4628-856c-2c10fb6e2c39" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Depreciation_983dcb61-ebf6-49f3-8a54-66c726a69c40" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Depreciation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_dd35902b-29c5-4ba2-992d-43c663a529bc" xlink:to="loc_us-gaap_Depreciation_983dcb61-ebf6-49f3-8a54-66c726a69c40" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AmortizationOfIntangibleAssets_bf898219-3532-4eb9-9487-6ceeb5e6a219" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AmortizationOfIntangibleAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_dd35902b-29c5-4ba2-992d-43c663a529bc" xlink:to="loc_us-gaap_AmortizationOfIntangibleAssets_bf898219-3532-4eb9-9487-6ceeb5e6a219" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_AmortizationOfAboveMarketLease_232901c0-b5f2-4754-a38a-f4ceec2322ea" xlink:href="land-20240630.xsd#land_AmortizationOfAboveMarketLease"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_dd35902b-29c5-4ba2-992d-43c663a529bc" xlink:to="loc_land_AmortizationOfAboveMarketLease_232901c0-b5f2-4754-a38a-f4ceec2322ea" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BelowMarketLeasePeriodIncreaseDecrease_7a89951f-a179-4c00-8095-6c8fd041e308" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BelowMarketLeasePeriodIncreaseDecrease"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_dd35902b-29c5-4ba2-992d-43c663a529bc" xlink:to="loc_us-gaap_BelowMarketLeasePeriodIncreaseDecrease_7a89951f-a179-4c00-8095-6c8fd041e308" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AreaOfRealEstateProperty_cf9c57c1-d00d-45eb-9ad1-352c7a4d3f7f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AreaOfRealEstateProperty"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_dd35902b-29c5-4ba2-992d-43c663a529bc" xlink:to="loc_us-gaap_AreaOfRealEstateProperty_cf9c57c1-d00d-45eb-9ad1-352c7a4d3f7f" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LandAvailableForSale_e18773e9-2f3d-4ef3-8553-5f77811f8ea6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LandAvailableForSale"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_dd35902b-29c5-4ba2-992d-43c663a529bc" xlink:to="loc_us-gaap_LandAvailableForSale_e18773e9-2f3d-4ef3-8553-5f77811f8ea6" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GainsLossesOnSalesOfInvestmentRealEstate_ba595ec9-b867-485a-9452-6d778e58feef" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_GainsLossesOnSalesOfInvestmentRealEstate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_dd35902b-29c5-4ba2-992d-43c663a529bc" xlink:to="loc_us-gaap_GainsLossesOnSalesOfInvestmentRealEstate_ba595ec9-b867-485a-9452-6d778e58feef" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LimitedLiabilityCompanyLLCOrLimitedPartnershipLPMembersOrLimitedPartnersOwnershipInterest_13923510-bae5-4a3f-9b90-272b904037ac" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LimitedLiabilityCompanyLLCOrLimitedPartnershipLPMembersOrLimitedPartnersOwnershipInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_dd35902b-29c5-4ba2-992d-43c663a529bc" xlink:to="loc_us-gaap_LimitedLiabilityCompanyLLCOrLimitedPartnershipLPMembersOrLimitedPartnersOwnershipInterest_13923510-bae5-4a3f-9b90-272b904037ac" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_AssetAcquisitionProFormaInformationEarningsLossOfAcquireeSinceAcquisitionDateActual_75db20f6-1f17-4912-b613-a4d52ae9b0cd" xlink:href="land-20240630.xsd#land_AssetAcquisitionProFormaInformationEarningsLossOfAcquireeSinceAcquisitionDateActual"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_dd35902b-29c5-4ba2-992d-43c663a529bc" xlink:to="loc_land_AssetAcquisitionProFormaInformationEarningsLossOfAcquireeSinceAcquisitionDateActual_75db20f6-1f17-4912-b613-a4d52ae9b0cd" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LimitedLiabilityCompanyLlcMembersEquityIncludingPortionAttributableToNoncontrollingInterest_29dc0d80-a010-4c94-b229-035190795d88" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LimitedLiabilityCompanyLlcMembersEquityIncludingPortionAttributableToNoncontrollingInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_dd35902b-29c5-4ba2-992d-43c663a529bc" xlink:to="loc_us-gaap_LimitedLiabilityCompanyLlcMembersEquityIncludingPortionAttributableToNoncontrollingInterest_29dc0d80-a010-4c94-b229-035190795d88" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_NumberOfContractsToPurchaseBankedWater_d17a71c3-133b-42d4-8b96-cba5d9707bea" xlink:href="land-20240630.xsd#land_NumberOfContractsToPurchaseBankedWater"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_dd35902b-29c5-4ba2-992d-43c663a529bc" xlink:to="loc_land_NumberOfContractsToPurchaseBankedWater_d17a71c3-133b-42d4-8b96-cba5d9707bea" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_AreaOfWater_b7ebafcc-c7dc-4d57-97a0-ffb7e5505d39" xlink:href="land-20240630.xsd#land_AreaOfWater"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_dd35902b-29c5-4ba2-992d-43c663a529bc" xlink:to="loc_land_AreaOfWater_b7ebafcc-c7dc-4d57-97a0-ffb7e5505d39" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireWaterSystems_2329c166-5657-4718-acbb-42fc9c65d370" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PaymentsToAcquireWaterSystems"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_dd35902b-29c5-4ba2-992d-43c663a529bc" xlink:to="loc_us-gaap_PaymentsToAcquireWaterSystems_2329c166-5657-4718-acbb-42fc9c65d370" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_WaterAssetsFairValueDisclosure_a872dd9b-200b-4aec-ad0e-a3d57de9860b" xlink:href="land-20240630.xsd#land_WaterAssetsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_dd35902b-29c5-4ba2-992d-43c663a529bc" xlink:to="loc_land_WaterAssetsFairValueDisclosure_a872dd9b-200b-4aec-ad0e-a3d57de9860b" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_GroundwaterRechargeProgramPercentageOfGroundwaterCredits_6f7d4d47-e954-4178-bb57-5be070255d1c" xlink:href="land-20240630.xsd#land_GroundwaterRechargeProgramPercentageOfGroundwaterCredits"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_dd35902b-29c5-4ba2-992d-43c663a529bc" xlink:to="loc_land_GroundwaterRechargeProgramPercentageOfGroundwaterCredits_6f7d4d47-e954-4178-bb57-5be070255d1c" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_AreaOfWaterWaterCreditsObtainedDuringPeriod_76ea2839-7626-4660-ae9d-d0558dad60cd" xlink:href="land-20240630.xsd#land_AreaOfWaterWaterCreditsObtainedDuringPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_dd35902b-29c5-4ba2-992d-43c663a529bc" xlink:to="loc_land_AreaOfWaterWaterCreditsObtainedDuringPeriod_76ea2839-7626-4660-ae9d-d0558dad60cd" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherOperatingIncome_c7b03769-4a99-485d-b0ef-96ea567e9311" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherOperatingIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_dd35902b-29c5-4ba2-992d-43c663a529bc" xlink:to="loc_us-gaap_OtherOperatingIncome_c7b03769-4a99-485d-b0ef-96ea567e9311" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DevelopmentInProcess_4f8beddd-b1ba-45e2-a4ef-a44667ef9b6f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DevelopmentInProcess"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_dd35902b-29c5-4ba2-992d-43c663a529bc" xlink:to="loc_us-gaap_DevelopmentInProcess_4f8beddd-b1ba-45e2-a4ef-a44667ef9b6f" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_DevelopmentInProcessDeferred_0d1d4ba2-c7a9-4d1d-84af-0a854c92a2cc" xlink:href="land-20240630.xsd#land_DevelopmentInProcessDeferred"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_dd35902b-29c5-4ba2-992d-43c663a529bc" xlink:to="loc_land_DevelopmentInProcessDeferred_0d1d4ba2-c7a9-4d1d-84af-0a854c92a2cc" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LeaseIncome_71512050-9541-4962-801e-d9d259219064" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LeaseIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_dd35902b-29c5-4ba2-992d-43c663a529bc" xlink:to="loc_us-gaap_LeaseIncome_71512050-9541-4962-801e-d9d259219064" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_PercentageOfRentalRevenue_e092c3a1-9dff-4adf-9279-df04a64a422e" xlink:href="land-20240630.xsd#land_PercentageOfRentalRevenue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_dd35902b-29c5-4ba2-992d-43c663a529bc" xlink:to="loc_land_PercentageOfRentalRevenue_e092c3a1-9dff-4adf-9279-df04a64a422e" xlink:type="arc" order="21"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingencyEstimateOfPossibleLoss_6cecc6f7-01d7-405d-8c74-8b3465e71f48" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LossContingencyEstimateOfPossibleLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_dd35902b-29c5-4ba2-992d-43c663a529bc" xlink:to="loc_us-gaap_LossContingencyEstimateOfPossibleLoss_6cecc6f7-01d7-405d-8c74-8b3465e71f48" xlink:type="arc" order="22"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_NumberOfImpairedProperties_305caa63-8e42-454f-9ce4-656865bab59f" xlink:href="land-20240630.xsd#land_NumberOfImpairedProperties"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_dd35902b-29c5-4ba2-992d-43c663a529bc" xlink:to="loc_land_NumberOfImpairedProperties_305caa63-8e42-454f-9ce4-656865bab59f" xlink:type="arc" order="23"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ImpairmentOfRealEstate_df00a0c4-a085-41d3-a196-ea8d8bf367ee" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ImpairmentOfRealEstate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_dd35902b-29c5-4ba2-992d-43c663a529bc" xlink:to="loc_us-gaap_ImpairmentOfRealEstate_df00a0c4-a085-41d3-a196-ea8d8bf367ee" xlink:type="arc" order="24"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.gladstoneland.com/role/RealEstateandIntangibleAssetsScheduleofInformationofFarmsDetails" xlink:type="simple" xlink:href="land-20240630.xsd#RealEstateandIntangibleAssetsScheduleofInformationofFarmsDetails"/>
  <link:definitionLink xlink:role="http://www.gladstoneland.com/role/RealEstateandIntangibleAssetsScheduleofInformationofFarmsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RealEstatePropertiesLineItems_cf07dc02-929a-49e0-bce3-a2c39a291690" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RealEstatePropertiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRealEstatePropertiesTable_3bf8a233-cdd7-4f8e-896d-628856352951" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfRealEstatePropertiesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_cf07dc02-929a-49e0-bce3-a2c39a291690" xlink:to="loc_us-gaap_ScheduleOfRealEstatePropertiesTable_3bf8a233-cdd7-4f8e-896d-628856352951" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_db7d0a1b-b490-45e3-a7ea-9e8bd63a0b64" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRealEstatePropertiesTable_3bf8a233-cdd7-4f8e-896d-628856352951" xlink:to="loc_srt_StatementGeographicalAxis_db7d0a1b-b490-45e3-a7ea-9e8bd63a0b64" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_db7d0a1b-b490-45e3-a7ea-9e8bd63a0b64_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_db7d0a1b-b490-45e3-a7ea-9e8bd63a0b64" xlink:to="loc_srt_SegmentGeographicalDomain_db7d0a1b-b490-45e3-a7ea-9e8bd63a0b64_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_eefdda42-07dd-48a3-903c-12e9c7b99dd9" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_db7d0a1b-b490-45e3-a7ea-9e8bd63a0b64" xlink:to="loc_srt_SegmentGeographicalDomain_eefdda42-07dd-48a3-903c-12e9c7b99dd9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_stpr_CA_ebe580d1-5eb4-4f95-91de-6a56ef61e255" xlink:href="https://xbrl.sec.gov/stpr/2024/stpr-2024.xsd#stpr_CA"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_eefdda42-07dd-48a3-903c-12e9c7b99dd9" xlink:to="loc_stpr_CA_ebe580d1-5eb4-4f95-91de-6a56ef61e255" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_KernCaliforniaMember_24f27e90-78cf-4784-a29e-2b897231f0b7" xlink:href="land-20240630.xsd#land_KernCaliforniaMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_stpr_CA_ebe580d1-5eb4-4f95-91de-6a56ef61e255" xlink:to="loc_land_KernCaliforniaMember_24f27e90-78cf-4784-a29e-2b897231f0b7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_FresnoCAMember_6d5506c6-b5fc-4114-82e2-28b330ec7555" xlink:href="land-20240630.xsd#land_FresnoCAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_stpr_CA_ebe580d1-5eb4-4f95-91de-6a56ef61e255" xlink:to="loc_land_FresnoCAMember_6d5506c6-b5fc-4114-82e2-28b330ec7555" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_stpr_FL_389e86df-8256-47fb-ab60-2afa078fa811" xlink:href="https://xbrl.sec.gov/stpr/2024/stpr-2024.xsd#stpr_FL"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_eefdda42-07dd-48a3-903c-12e9c7b99dd9" xlink:to="loc_stpr_FL_389e86df-8256-47fb-ab60-2afa078fa811" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_stpr_WA_9e319158-cb50-47e9-b5c9-c8a099a57687" xlink:href="https://xbrl.sec.gov/stpr/2024/stpr-2024.xsd#stpr_WA"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_eefdda42-07dd-48a3-903c-12e9c7b99dd9" xlink:to="loc_stpr_WA_9e319158-cb50-47e9-b5c9-c8a099a57687" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_stpr_AZ_dfd23503-4b25-4419-9262-eca7916428d4" xlink:href="https://xbrl.sec.gov/stpr/2024/stpr-2024.xsd#stpr_AZ"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_eefdda42-07dd-48a3-903c-12e9c7b99dd9" xlink:to="loc_stpr_AZ_dfd23503-4b25-4419-9262-eca7916428d4" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_stpr_CO_1e68e5af-9e5a-408d-9451-511ddda09639" xlink:href="https://xbrl.sec.gov/stpr/2024/stpr-2024.xsd#stpr_CO"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_eefdda42-07dd-48a3-903c-12e9c7b99dd9" xlink:to="loc_stpr_CO_1e68e5af-9e5a-408d-9451-511ddda09639" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_stpr_NE_1b86cfe5-8fd0-4eec-905e-01c37d25fd26" xlink:href="https://xbrl.sec.gov/stpr/2024/stpr-2024.xsd#stpr_NE"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_eefdda42-07dd-48a3-903c-12e9c7b99dd9" xlink:to="loc_stpr_NE_1b86cfe5-8fd0-4eec-905e-01c37d25fd26" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_stpr_OR_4e079a6c-1388-4814-8b95-c608838bf3f7" xlink:href="https://xbrl.sec.gov/stpr/2024/stpr-2024.xsd#stpr_OR"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_eefdda42-07dd-48a3-903c-12e9c7b99dd9" xlink:to="loc_stpr_OR_4e079a6c-1388-4814-8b95-c608838bf3f7" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_stpr_MI_4cf7296b-d683-4480-a514-303168f7adc0" xlink:href="https://xbrl.sec.gov/stpr/2024/stpr-2024.xsd#stpr_MI"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_eefdda42-07dd-48a3-903c-12e9c7b99dd9" xlink:to="loc_stpr_MI_4cf7296b-d683-4480-a514-303168f7adc0" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_stpr_TX_e1455555-b9ff-4ee2-aea0-b1a406ab7f5b" xlink:href="https://xbrl.sec.gov/stpr/2024/stpr-2024.xsd#stpr_TX"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_eefdda42-07dd-48a3-903c-12e9c7b99dd9" xlink:to="loc_stpr_TX_e1455555-b9ff-4ee2-aea0-b1a406ab7f5b" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_stpr_MD_021c14f6-6d69-44dc-b101-3cd0edca5a7a" xlink:href="https://xbrl.sec.gov/stpr/2024/stpr-2024.xsd#stpr_MD"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_eefdda42-07dd-48a3-903c-12e9c7b99dd9" xlink:to="loc_stpr_MD_021c14f6-6d69-44dc-b101-3cd0edca5a7a" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_stpr_SC_f5671133-393a-4d35-9dc4-4599bf3f6177" xlink:href="https://xbrl.sec.gov/stpr/2024/stpr-2024.xsd#stpr_SC"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_eefdda42-07dd-48a3-903c-12e9c7b99dd9" xlink:to="loc_stpr_SC_f5671133-393a-4d35-9dc4-4599bf3f6177" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_stpr_GA_4014ad0e-2721-445e-a64b-1aa54ae0009b" xlink:href="https://xbrl.sec.gov/stpr/2024/stpr-2024.xsd#stpr_GA"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_eefdda42-07dd-48a3-903c-12e9c7b99dd9" xlink:to="loc_stpr_GA_4014ad0e-2721-445e-a64b-1aa54ae0009b" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_stpr_NC_79460dea-3a9d-498d-b7ab-b2bbf98b4e19" xlink:href="https://xbrl.sec.gov/stpr/2024/stpr-2024.xsd#stpr_NC"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_eefdda42-07dd-48a3-903c-12e9c7b99dd9" xlink:to="loc_stpr_NC_79460dea-3a9d-498d-b7ab-b2bbf98b4e19" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_stpr_NJ_9f4d892d-597d-4435-be00-7fc93d8393d7" xlink:href="https://xbrl.sec.gov/stpr/2024/stpr-2024.xsd#stpr_NJ"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_eefdda42-07dd-48a3-903c-12e9c7b99dd9" xlink:to="loc_stpr_NJ_9f4d892d-597d-4435-be00-7fc93d8393d7" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_stpr_DE_266e41da-124c-4451-b120-9fc42dda68c7" xlink:href="https://xbrl.sec.gov/stpr/2024/stpr-2024.xsd#stpr_DE"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_eefdda42-07dd-48a3-903c-12e9c7b99dd9" xlink:to="loc_stpr_DE_266e41da-124c-4451-b120-9fc42dda68c7" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CounterpartyNameAxis_f9db0be8-2e7b-45d2-914e-9e1982d6b58d" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_CounterpartyNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRealEstatePropertiesTable_3bf8a233-cdd7-4f8e-896d-628856352951" xlink:to="loc_srt_CounterpartyNameAxis_f9db0be8-2e7b-45d2-914e-9e1982d6b58d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_f9db0be8-2e7b-45d2-914e-9e1982d6b58d_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CounterpartyNameAxis_f9db0be8-2e7b-45d2-914e-9e1982d6b58d" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_f9db0be8-2e7b-45d2-914e-9e1982d6b58d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_c0af1c0b-3aaa-4f54-8327-810decb44b88" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CounterpartyNameAxis_f9db0be8-2e7b-45d2-914e-9e1982d6b58d" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_c0af1c0b-3aaa-4f54-8327-810decb44b88" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_StateOfCaliforniaMember_39a6d49f-3629-4910-ac1a-c01da3cf4023" xlink:href="land-20240630.xsd#land_StateOfCaliforniaMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_c0af1c0b-3aaa-4f54-8327-810decb44b88" xlink:to="loc_land_StateOfCaliforniaMember_39a6d49f-3629-4910-ac1a-c01da3cf4023" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_StateofArizonaMember_abf0a43a-587a-4173-bac6-b985f3725039" xlink:href="land-20240630.xsd#land_StateofArizonaMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_c0af1c0b-3aaa-4f54-8327-810decb44b88" xlink:to="loc_land_StateofArizonaMember_abf0a43a-587a-4173-bac6-b985f3725039" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_5c3d06cb-f47a-4a3c-8c2b-1fb88c0feaf0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRealEstatePropertiesTable_3bf8a233-cdd7-4f8e-896d-628856352951" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_5c3d06cb-f47a-4a3c-8c2b-1fb88c0feaf0" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_5c3d06cb-f47a-4a3c-8c2b-1fb88c0feaf0_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_5c3d06cb-f47a-4a3c-8c2b-1fb88c0feaf0" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_5c3d06cb-f47a-4a3c-8c2b-1fb88c0feaf0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_41b597e5-5818-4a1c-a5e3-3f0b85e399ff" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_5c3d06cb-f47a-4a3c-8c2b-1fb88c0feaf0" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_41b597e5-5818-4a1c-a5e3-3f0b85e399ff" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LeasesAcquiredInPlaceMember_17ab2aae-e7ec-420b-9faf-65322147df84" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LeasesAcquiredInPlaceMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_41b597e5-5818-4a1c-a5e3-3f0b85e399ff" xlink:to="loc_us-gaap_LeasesAcquiredInPlaceMember_17ab2aae-e7ec-420b-9faf-65322147df84" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_NumberOfRealEstateFarms_a03633d8-4a3e-4b45-a25e-bd2e1cbbfcf0" xlink:href="land-20240630.xsd#land_NumberOfRealEstateFarms"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_cf07dc02-929a-49e0-bce3-a2c39a291690" xlink:to="loc_land_NumberOfRealEstateFarms_a03633d8-4a3e-4b45-a25e-bd2e1cbbfcf0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AreaOfRealEstateProperty_ae2ad48b-43c9-4a56-9bfd-e10f7425913e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AreaOfRealEstateProperty"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_cf07dc02-929a-49e0-bce3-a2c39a291690" xlink:to="loc_us-gaap_AreaOfRealEstateProperty_ae2ad48b-43c9-4a56-9bfd-e10f7425913e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_FarmableAreaOfLand_aa184270-c458-4543-a331-5e654ece4b14" xlink:href="land-20240630.xsd#land_FarmableAreaOfLand"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_cf07dc02-929a-49e0-bce3-a2c39a291690" xlink:to="loc_land_FarmableAreaOfLand_aa184270-c458-4543-a331-5e654ece4b14" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_AreaOfWater_512bc841-a056-43ae-bbaa-d8978416a894" xlink:href="land-20240630.xsd#land_AreaOfWater"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_cf07dc02-929a-49e0-bce3-a2c39a291690" xlink:to="loc_land_AreaOfWater_512bc841-a056-43ae-bbaa-d8978416a894" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_SecScheduleIiiRealEstateInvestmentPropertyIncludingIntangibleAssetsNet_2b1ffac9-48fa-4488-abcd-e560baa45d4f" xlink:href="land-20240630.xsd#land_SecScheduleIiiRealEstateInvestmentPropertyIncludingIntangibleAssetsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_cf07dc02-929a-49e0-bce3-a2c39a291690" xlink:to="loc_land_SecScheduleIiiRealEstateInvestmentPropertyIncludingIntangibleAssetsNet_2b1ffac9-48fa-4488-abcd-e560baa45d4f" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RealEstateAndAccumulatedDepreciationAmountOfEncumbrances_f505d479-e5a0-4797-a600-051fef34fd39" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RealEstateAndAccumulatedDepreciationAmountOfEncumbrances"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_cf07dc02-929a-49e0-bce3-a2c39a291690" xlink:to="loc_srt_RealEstateAndAccumulatedDepreciationAmountOfEncumbrances_f505d479-e5a0-4797-a600-051fef34fd39" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredFinanceCostsNet_098cb26d-9ec5-402f-b7b2-ba12a108c6c2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredFinanceCostsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_cf07dc02-929a-49e0-bce3-a2c39a291690" xlink:to="loc_us-gaap_DeferredFinanceCostsNet_098cb26d-9ec5-402f-b7b2-ba12a108c6c2" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LimitedLiabilityCompanyLlcMembersEquityIncludingPortionAttributableToNoncontrollingInterest_d5d4aa06-75f1-4b15-b084-3c3685f27c21" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LimitedLiabilityCompanyLlcMembersEquityIncludingPortionAttributableToNoncontrollingInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_cf07dc02-929a-49e0-bce3-a2c39a291690" xlink:to="loc_us-gaap_LimitedLiabilityCompanyLlcMembersEquityIncludingPortionAttributableToNoncontrollingInterest_d5d4aa06-75f1-4b15-b084-3c3685f27c21" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_NumberOfLeases_175aeafd-b62c-417b-abd1-9a052ed3257d" xlink:href="land-20240630.xsd#land_NumberOfLeases"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_cf07dc02-929a-49e0-bce3-a2c39a291690" xlink:to="loc_land_NumberOfLeases_175aeafd-b62c-417b-abd1-9a052ed3257d" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_NumberOfFarmsWaterProvided_1df080f9-fbe7-4e47-b2b2-559c7664b527" xlink:href="land-20240630.xsd#land_NumberOfFarmsWaterProvided"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_cf07dc02-929a-49e0-bce3-a2c39a291690" xlink:to="loc_land_NumberOfFarmsWaterProvided_1df080f9-fbe7-4e47-b2b2-559c7664b527" xlink:type="arc" order="9"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.gladstoneland.com/role/RealEstateandIntangibleAssetsCarryingValueofLeaseIntangiblesandAccumulatedAmortizationDetails" xlink:type="simple" xlink:href="land-20240630.xsd#RealEstateandIntangibleAssetsCarryingValueofLeaseIntangiblesandAccumulatedAmortizationDetails"/>
  <link:definitionLink xlink:role="http://www.gladstoneland.com/role/RealEstateandIntangibleAssetsCarryingValueofLeaseIntangiblesandAccumulatedAmortizationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_767197e6-b27f-4b4c-8b24-d3063d467c2c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_144e9237-fa9d-4f4c-ad1f-28322ab4fcdd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_767197e6-b27f-4b4c-8b24-d3063d467c2c" xlink:to="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_144e9237-fa9d-4f4c-ad1f-28322ab4fcdd" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_9422afdb-91db-4a50-9ef0-fa1c824da94c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_144e9237-fa9d-4f4c-ad1f-28322ab4fcdd" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_9422afdb-91db-4a50-9ef0-fa1c824da94c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_9422afdb-91db-4a50-9ef0-fa1c824da94c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_9422afdb-91db-4a50-9ef0-fa1c824da94c" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_9422afdb-91db-4a50-9ef0-fa1c824da94c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_af05a908-1798-40dd-a13b-0cf0af61fbde" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_9422afdb-91db-4a50-9ef0-fa1c824da94c" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_af05a908-1798-40dd-a13b-0cf0af61fbde" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_LeaseIntangiblesMember_1b92b61f-7579-4835-bfe4-cb051f87e8f7" xlink:href="land-20240630.xsd#land_LeaseIntangiblesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_af05a908-1798-40dd-a13b-0cf0af61fbde" xlink:to="loc_land_LeaseIntangiblesMember_1b92b61f-7579-4835-bfe4-cb051f87e8f7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LeaseAgreementsMember_3c217cec-97a3-4fa3-a02a-85afe2cb74b7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LeaseAgreementsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_land_LeaseIntangiblesMember_1b92b61f-7579-4835-bfe4-cb051f87e8f7" xlink:to="loc_us-gaap_LeaseAgreementsMember_3c217cec-97a3-4fa3-a02a-85afe2cb74b7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LeasesAcquiredInPlaceMember_b193a9a6-3303-473b-ac6b-21fbcf8c806a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LeasesAcquiredInPlaceMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_land_LeaseIntangiblesMember_1b92b61f-7579-4835-bfe4-cb051f87e8f7" xlink:to="loc_us-gaap_LeasesAcquiredInPlaceMember_b193a9a6-3303-473b-ac6b-21fbcf8c806a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_LeasingCostsMember_dbf09779-d2e9-4f8b-8f39-a2dd3e59e341" xlink:href="land-20240630.xsd#land_LeasingCostsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_land_LeaseIntangiblesMember_1b92b61f-7579-4835-bfe4-cb051f87e8f7" xlink:to="loc_land_LeasingCostsMember_dbf09779-d2e9-4f8b-8f39-a2dd3e59e341" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherIntangibleAssetsMember_a93ec4cc-df3e-48cd-903f-df69e430ef33" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherIntangibleAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_land_LeaseIntangiblesMember_1b92b61f-7579-4835-bfe4-cb051f87e8f7" xlink:to="loc_us-gaap_OtherIntangibleAssetsMember_a93ec4cc-df3e-48cd-903f-df69e430ef33" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsGross_e7de852d-b8a3-4f96-a11b-3fbda40e753d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_767197e6-b27f-4b4c-8b24-d3063d467c2c" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsGross_e7de852d-b8a3-4f96-a11b-3fbda40e753d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_b4132c4b-0314-4014-bb01-7dc5b0452549" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_767197e6-b27f-4b4c-8b24-d3063d467c2c" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_b4132c4b-0314-4014-bb01-7dc5b0452549" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsNet_1a2ab3e4-627a-4678-8c54-de6925e6d3a4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_767197e6-b27f-4b4c-8b24-d3063d467c2c" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsNet_1a2ab3e4-627a-4678-8c54-de6925e6d3a4" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.gladstoneland.com/role/RealEstateandIntangibleAssetsCarryingValueofLeaseIntangibleAssetsorLiabilitiesinOtherAssetsandOtherLiabilitiesDetails" xlink:type="simple" xlink:href="land-20240630.xsd#RealEstateandIntangibleAssetsCarryingValueofLeaseIntangibleAssetsorLiabilitiesinOtherAssetsandOtherLiabilitiesDetails"/>
  <link:definitionLink xlink:role="http://www.gladstoneland.com/role/RealEstateandIntangibleAssetsCarryingValueofLeaseIntangibleAssetsorLiabilitiesinOtherAssetsandOtherLiabilitiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_d2a632ae-268c-4d80-b770-04861dfce543" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_d93768ba-1f19-46f7-9b54-11464f97e2d7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_d2a632ae-268c-4d80-b770-04861dfce543" xlink:to="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_d93768ba-1f19-46f7-9b54-11464f97e2d7" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_5a1591a6-3882-4ae0-94ef-3fed125fee8e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_d93768ba-1f19-46f7-9b54-11464f97e2d7" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_5a1591a6-3882-4ae0-94ef-3fed125fee8e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_5a1591a6-3882-4ae0-94ef-3fed125fee8e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_5a1591a6-3882-4ae0-94ef-3fed125fee8e" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_5a1591a6-3882-4ae0-94ef-3fed125fee8e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_0fbe4361-8003-443b-846b-00d106f188f4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_5a1591a6-3882-4ae0-94ef-3fed125fee8e" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_0fbe4361-8003-443b-846b-00d106f188f4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AboveMarketLeasesMember_f8687ce1-d478-443b-b4e5-9058457390f3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AboveMarketLeasesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_0fbe4361-8003-443b-846b-00d106f188f4" xlink:to="loc_us-gaap_AboveMarketLeasesMember_f8687ce1-d478-443b-b4e5-9058457390f3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_BelowMarketLeasesAndDeferredRevenueMember_813ff2e7-41aa-4a80-9a22-3df261574d0c" xlink:href="land-20240630.xsd#land_BelowMarketLeasesAndDeferredRevenueMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_0fbe4361-8003-443b-846b-00d106f188f4" xlink:to="loc_land_BelowMarketLeasesAndDeferredRevenueMember_813ff2e7-41aa-4a80-9a22-3df261574d0c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_AboveAndBelowMarketLeasesMember_1a9b3005-00f6-4bde-847b-f51c21b1e2f7" xlink:href="land-20240630.xsd#land_AboveAndBelowMarketLeasesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_0fbe4361-8003-443b-846b-00d106f188f4" xlink:to="loc_land_AboveAndBelowMarketLeasesMember_1a9b3005-00f6-4bde-847b-f51c21b1e2f7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsGross_9360098d-4e6a-462b-baba-26d647c5e17f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_d2a632ae-268c-4d80-b770-04861dfce543" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsGross_9360098d-4e6a-462b-baba-26d647c5e17f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_b8850e7b-f1a9-4fa0-aa28-58d7133e9f32" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_d2a632ae-268c-4d80-b770-04861dfce543" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_b8850e7b-f1a9-4fa0-aa28-58d7133e9f32" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BelowMarketLeaseGross_df911a34-bfeb-4659-8e02-6ba422ef5ecb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BelowMarketLeaseGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_d2a632ae-268c-4d80-b770-04861dfce543" xlink:to="loc_us-gaap_BelowMarketLeaseGross_df911a34-bfeb-4659-8e02-6ba422ef5ecb" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BelowMarketLeaseAccumulatedAmortization_91a7002f-59af-47cd-89e5-aa8a66002433" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BelowMarketLeaseAccumulatedAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_d2a632ae-268c-4d80-b770-04861dfce543" xlink:to="loc_us-gaap_BelowMarketLeaseAccumulatedAmortization_91a7002f-59af-47cd-89e5-aa8a66002433" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_FiniteLivedIntangibleAssetsLiabilitiesGross_18b2b7d1-f004-4979-bee8-3f8c9f1f265b" xlink:href="land-20240630.xsd#land_FiniteLivedIntangibleAssetsLiabilitiesGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_d2a632ae-268c-4d80-b770-04861dfce543" xlink:to="loc_land_FiniteLivedIntangibleAssetsLiabilitiesGross_18b2b7d1-f004-4979-bee8-3f8c9f1f265b" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_FiniteLivedIntangibleAssetsAccumulatedAmortizationAndAccretion_15c443ab-c2b4-4165-8ee9-a3a4b0b94506" xlink:href="land-20240630.xsd#land_FiniteLivedIntangibleAssetsAccumulatedAmortizationAndAccretion"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_d2a632ae-268c-4d80-b770-04861dfce543" xlink:to="loc_land_FiniteLivedIntangibleAssetsAccumulatedAmortizationAndAccretion_15c443ab-c2b4-4165-8ee9-a3a4b0b94506" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.gladstoneland.com/role/RealEstateandIntangibleAssetsSummaryofAcreFeetObtainedUponExercisingContractsDetails" xlink:type="simple" xlink:href="land-20240630.xsd#RealEstateandIntangibleAssetsSummaryofAcreFeetObtainedUponExercisingContractsDetails"/>
  <link:definitionLink xlink:role="http://www.gladstoneland.com/role/RealEstateandIntangibleAssetsSummaryofAcreFeetObtainedUponExercisingContractsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RealEstatePropertiesLineItems_1650f181-c268-4bff-825b-f1ed8d3daceb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RealEstatePropertiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRealEstatePropertiesTable_7b976a5c-f7fa-4906-bdaa-0f1a5b106431" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfRealEstatePropertiesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_1650f181-c268-4bff-825b-f1ed8d3daceb" xlink:to="loc_us-gaap_ScheduleOfRealEstatePropertiesTable_7b976a5c-f7fa-4906-bdaa-0f1a5b106431" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CounterpartyNameAxis_d0013c46-9cf2-4711-a67c-7d4bdcf4dc6e" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_CounterpartyNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRealEstatePropertiesTable_7b976a5c-f7fa-4906-bdaa-0f1a5b106431" xlink:to="loc_srt_CounterpartyNameAxis_d0013c46-9cf2-4711-a67c-7d4bdcf4dc6e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_d0013c46-9cf2-4711-a67c-7d4bdcf4dc6e_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CounterpartyNameAxis_d0013c46-9cf2-4711-a67c-7d4bdcf4dc6e" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_d0013c46-9cf2-4711-a67c-7d4bdcf4dc6e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_be0d6843-b55a-462b-992e-f94d38bb4504" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CounterpartyNameAxis_d0013c46-9cf2-4711-a67c-7d4bdcf4dc6e" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_be0d6843-b55a-462b-992e-f94d38bb4504" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_SemitropicWaterStorageDistrictMember_75a81332-078d-4620-b1d8-49cbcc07035a" xlink:href="land-20240630.xsd#land_SemitropicWaterStorageDistrictMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_be0d6843-b55a-462b-992e-f94d38bb4504" xlink:to="loc_land_SemitropicWaterStorageDistrictMember_75a81332-078d-4620-b1d8-49cbcc07035a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_50a64aaf-b9ab-46bb-8101-472ca8e20dc2" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRealEstatePropertiesTable_7b976a5c-f7fa-4906-bdaa-0f1a5b106431" xlink:to="loc_srt_StatementGeographicalAxis_50a64aaf-b9ab-46bb-8101-472ca8e20dc2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_50a64aaf-b9ab-46bb-8101-472ca8e20dc2_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_50a64aaf-b9ab-46bb-8101-472ca8e20dc2" xlink:to="loc_srt_SegmentGeographicalDomain_50a64aaf-b9ab-46bb-8101-472ca8e20dc2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_0f5a4e29-3cec-4fcb-a88b-2eeb40955361" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_50a64aaf-b9ab-46bb-8101-472ca8e20dc2" xlink:to="loc_srt_SegmentGeographicalDomain_0f5a4e29-3cec-4fcb-a88b-2eeb40955361" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_KernCaliforniaMember_d4c0a2a8-0bd0-4506-b3ba-113f266d2b05" xlink:href="land-20240630.xsd#land_KernCaliforniaMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_0f5a4e29-3cec-4fcb-a88b-2eeb40955361" xlink:to="loc_land_KernCaliforniaMember_d4c0a2a8-0bd0-4506-b3ba-113f266d2b05" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_AreaOfWaterAvailableForPurchaseContract_11034c7b-c0eb-4228-9d17-8a1410e6a8f9" xlink:href="land-20240630.xsd#land_AreaOfWaterAvailableForPurchaseContract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_1650f181-c268-4bff-825b-f1ed8d3daceb" xlink:to="loc_land_AreaOfWaterAvailableForPurchaseContract_11034c7b-c0eb-4228-9d17-8a1410e6a8f9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_AreaOfWaterPurchased_07f8d2f8-a9c9-4b55-be11-94fbca0e0940" xlink:href="land-20240630.xsd#land_AreaOfWaterPurchased"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_1650f181-c268-4bff-825b-f1ed8d3daceb" xlink:to="loc_land_AreaOfWaterPurchased_07f8d2f8-a9c9-4b55-be11-94fbca0e0940" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_WaterAssetsValueOfPurchaseContract_7c169bd1-0f63-428d-9fda-225d6ef5d508" xlink:href="land-20240630.xsd#land_WaterAssetsValueOfPurchaseContract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_1650f181-c268-4bff-825b-f1ed8d3daceb" xlink:to="loc_land_WaterAssetsValueOfPurchaseContract_7c169bd1-0f63-428d-9fda-225d6ef5d508" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_WaterAssetsCostToExerciseContract_c5f51b24-0940-4d85-ab67-0f9c3ef17a43" xlink:href="land-20240630.xsd#land_WaterAssetsCostToExerciseContract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_1650f181-c268-4bff-825b-f1ed8d3daceb" xlink:to="loc_land_WaterAssetsCostToExerciseContract_c5f51b24-0940-4d85-ab67-0f9c3ef17a43" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_WaterAssetsPurchasedCarryingValue_2c4aaf49-7f9b-426a-b874-05fa418e4bf4" xlink:href="land-20240630.xsd#land_WaterAssetsPurchasedCarryingValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RealEstatePropertiesLineItems_1650f181-c268-4bff-825b-f1ed8d3daceb" xlink:to="loc_land_WaterAssetsPurchasedCarryingValue_2c4aaf49-7f9b-426a-b874-05fa418e4bf4" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.gladstoneland.com/role/BorrowingsScheduleofBorrowingsDetails" xlink:type="simple" xlink:href="land-20240630.xsd#BorrowingsScheduleofBorrowingsDetails"/>
  <link:definitionLink xlink:role="http://www.gladstoneland.com/role/BorrowingsScheduleofBorrowingsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_2f81b06b-93bb-472a-aa5b-c71cee41dc54" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_7f20cb5d-2742-4592-98aa-42a4fa9db4b5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_2f81b06b-93bb-472a-aa5b-c71cee41dc54" xlink:to="loc_us-gaap_DebtInstrumentTable_7f20cb5d-2742-4592-98aa-42a4fa9db4b5" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_edfdedb2-9e22-4416-8b17-d14dc68a6554" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_7f20cb5d-2742-4592-98aa-42a4fa9db4b5" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_edfdedb2-9e22-4416-8b17-d14dc68a6554" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_edfdedb2-9e22-4416-8b17-d14dc68a6554_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_edfdedb2-9e22-4416-8b17-d14dc68a6554" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_edfdedb2-9e22-4416-8b17-d14dc68a6554_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_75176891-4e18-4b41-95a8-68b21e04f91b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_edfdedb2-9e22-4416-8b17-d14dc68a6554" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_75176891-4e18-4b41-95a8-68b21e04f91b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditMember_7c159969-9216-4ea3-8635-127f648b1ce5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LineOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_75176891-4e18-4b41-95a8-68b21e04f91b" xlink:to="loc_us-gaap_LineOfCreditMember_7c159969-9216-4ea3-8635-127f648b1ce5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NotesPayableToBanksMember_282fa62b-a3a5-43cc-bf90-8c333452ddf0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NotesPayableToBanksMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_75176891-4e18-4b41-95a8-68b21e04f91b" xlink:to="loc_us-gaap_NotesPayableToBanksMember_282fa62b-a3a5-43cc-bf90-8c333452ddf0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuredDebtMember_0ce7c2e4-e836-4382-83c4-4e4808b0595b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SecuredDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_75176891-4e18-4b41-95a8-68b21e04f91b" xlink:to="loc_us-gaap_SecuredDebtMember_0ce7c2e4-e836-4382-83c4-4e4808b0595b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_f78cec10-3da8-4229-9e6e-4784f1ca5db9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_7f20cb5d-2742-4592-98aa-42a4fa9db4b5" xlink:to="loc_us-gaap_DebtInstrumentAxis_f78cec10-3da8-4229-9e6e-4784f1ca5db9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_f78cec10-3da8-4229-9e6e-4784f1ca5db9_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_f78cec10-3da8-4229-9e6e-4784f1ca5db9" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_f78cec10-3da8-4229-9e6e-4784f1ca5db9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_b33ff701-04b3-4ee2-9805-6579c6d513cc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_f78cec10-3da8-4229-9e6e-4784f1ca5db9" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_b33ff701-04b3-4ee2-9805-6579c6d513cc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_VariablerateLineofCreditMember_8b0455ef-d719-4064-92bd-71aed555c4a3" xlink:href="land-20240630.xsd#land_VariablerateLineofCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_b33ff701-04b3-4ee2-9805-6579c6d513cc" xlink:to="loc_land_VariablerateLineofCreditMember_8b0455ef-d719-4064-92bd-71aed555c4a3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_LongTermMortgageNotesAndBondsPayableMember_cbd56510-b1e6-4be8-b31b-659eb46d0d8d" xlink:href="land-20240630.xsd#land_LongTermMortgageNotesAndBondsPayableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_b33ff701-04b3-4ee2-9805-6579c6d513cc" xlink:to="loc_land_LongTermMortgageNotesAndBondsPayableMember_cbd56510-b1e6-4be8-b31b-659eb46d0d8d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_FixedrateMortgageNotesPayableMember_f40af5fd-7bae-49e7-8bfa-e8885ab53689" xlink:href="land-20240630.xsd#land_FixedrateMortgageNotesPayableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_land_LongTermMortgageNotesAndBondsPayableMember_cbd56510-b1e6-4be8-b31b-659eb46d0d8d" xlink:to="loc_land_FixedrateMortgageNotesPayableMember_f40af5fd-7bae-49e7-8bfa-e8885ab53689" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_FixedRateBondsPayableMember_a5771de3-d9f7-4f2f-a18a-dc692bd16e6f" xlink:href="land-20240630.xsd#land_FixedRateBondsPayableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_land_LongTermMortgageNotesAndBondsPayableMember_cbd56510-b1e6-4be8-b31b-659eb46d0d8d" xlink:to="loc_land_FixedRateBondsPayableMember_a5771de3-d9f7-4f2f-a18a-dc692bd16e6f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityAxis_8d53e375-4bd9-4096-b1dc-974925e00d68" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_7f20cb5d-2742-4592-98aa-42a4fa9db4b5" xlink:to="loc_us-gaap_CreditFacilityAxis_8d53e375-4bd9-4096-b1dc-974925e00d68" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_8d53e375-4bd9-4096-b1dc-974925e00d68_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CreditFacilityAxis_8d53e375-4bd9-4096-b1dc-974925e00d68" xlink:to="loc_us-gaap_CreditFacilityDomain_8d53e375-4bd9-4096-b1dc-974925e00d68_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_de31d57a-ef21-4a21-bbc9-05b3f45d9fc2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CreditFacilityAxis_8d53e375-4bd9-4096-b1dc-974925e00d68" xlink:to="loc_us-gaap_CreditFacilityDomain_de31d57a-ef21-4a21-bbc9-05b3f45d9fc2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevolvingCreditFacilityMember_c8ee1851-d42f-4cf4-bb08-78da0dbe22af" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RevolvingCreditFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_de31d57a-ef21-4a21-bbc9-05b3f45d9fc2" xlink:to="loc_us-gaap_RevolvingCreditFacilityMember_c8ee1851-d42f-4cf4-bb08-78da0dbe22af" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_8e76c53b-4b1c-4255-9030-a9914427f9f8" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_7f20cb5d-2742-4592-98aa-42a4fa9db4b5" xlink:to="loc_srt_RangeAxis_8e76c53b-4b1c-4255-9030-a9914427f9f8" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_8e76c53b-4b1c-4255-9030-a9914427f9f8_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_8e76c53b-4b1c-4255-9030-a9914427f9f8" xlink:to="loc_srt_RangeMember_8e76c53b-4b1c-4255-9030-a9914427f9f8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_64ffcf48-795f-432f-90d3-64c8c7de2ca5" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_8e76c53b-4b1c-4255-9030-a9914427f9f8" xlink:to="loc_srt_RangeMember_64ffcf48-795f-432f-90d3-64c8c7de2ca5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_568ae5ce-f7f3-49a2-87c0-0c6a4be4544b" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_64ffcf48-795f-432f-90d3-64c8c7de2ca5" xlink:to="loc_srt_MinimumMember_568ae5ce-f7f3-49a2-87c0-0c6a4be4544b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_965ed3bf-64a2-47c1-bf4a-2228b03eaa57" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_64ffcf48-795f-432f-90d3-64c8c7de2ca5" xlink:to="loc_srt_MaximumMember_965ed3bf-64a2-47c1-bf4a-2228b03eaa57" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_WeightedAverageMember_ed1271d1-71b1-49b0-80f0-ad1b0a8a438c" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_WeightedAverageMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_64ffcf48-795f-432f-90d3-64c8c7de2ca5" xlink:to="loc_srt_WeightedAverageMember_ed1271d1-71b1-49b0-80f0-ad1b0a8a438c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentCarryingAmount_7900c394-ccfb-496d-b10a-7bf4eebf8dd3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentCarryingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_2f81b06b-93bb-472a-aa5b-c71cee41dc54" xlink:to="loc_us-gaap_DebtInstrumentCarryingAmount_7900c394-ccfb-496d-b10a-7bf4eebf8dd3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredFinanceCostsNet_d3a8960d-0bf9-4084-bc2d-0c5177adb279" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredFinanceCostsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_2f81b06b-93bb-472a-aa5b-c71cee41dc54" xlink:to="loc_us-gaap_DeferredFinanceCostsNet_d3a8960d-0bf9-4084-bc2d-0c5177adb279" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_de4021f7-ce40-4031-81d3-12a45aa4088e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_2f81b06b-93bb-472a-aa5b-c71cee41dc54" xlink:to="loc_us-gaap_LongTermDebt_de4021f7-ce40-4031-81d3-12a45aa4088e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_051999ad-934f-4922-9875-51aca72ad4f1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentInterestRateStatedPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_2f81b06b-93bb-472a-aa5b-c71cee41dc54" xlink:to="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_051999ad-934f-4922-9875-51aca72ad4f1" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.gladstoneland.com/role/BorrowingsAdditionalInformationDetails" xlink:type="simple" xlink:href="land-20240630.xsd#BorrowingsAdditionalInformationDetails"/>
  <link:definitionLink xlink:role="http://www.gladstoneland.com/role/BorrowingsAdditionalInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_e7954b5b-76ed-4d04-8467-222960b57448" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_e9678a26-7a02-4851-accd-34ea0a5b905f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e7954b5b-76ed-4d04-8467-222960b57448" xlink:to="loc_us-gaap_DebtInstrumentTable_e9678a26-7a02-4851-accd-34ea0a5b905f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_eb1e5669-7587-475d-acb3-d704bd8154b6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_e9678a26-7a02-4851-accd-34ea0a5b905f" xlink:to="loc_us-gaap_DebtInstrumentAxis_eb1e5669-7587-475d-acb3-d704bd8154b6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_eb1e5669-7587-475d-acb3-d704bd8154b6_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_eb1e5669-7587-475d-acb3-d704bd8154b6" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_eb1e5669-7587-475d-acb3-d704bd8154b6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_f1faf39a-6e84-402d-a722-3975fc92a06c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_eb1e5669-7587-475d-acb3-d704bd8154b6" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_f1faf39a-6e84-402d-a722-3975fc92a06c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_NotesandBondsBorrowingsMember_2b7f647d-e798-4dc6-9700-491468bca968" xlink:href="land-20240630.xsd#land_NotesandBondsBorrowingsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_f1faf39a-6e84-402d-a722-3975fc92a06c" xlink:to="loc_land_NotesandBondsBorrowingsMember_2b7f647d-e798-4dc6-9700-491468bca968" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_FarmCreditNotesPayableMember_9c760e43-fc1b-469f-8c2a-f04030f1f975" xlink:href="land-20240630.xsd#land_FarmCreditNotesPayableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_f1faf39a-6e84-402d-a722-3975fc92a06c" xlink:to="loc_land_FarmCreditNotesPayableMember_9c760e43-fc1b-469f-8c2a-f04030f1f975" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_MetLifeTermLoan2020Member_3bc49943-e4a3-4d37-8279-c385dbc8dd5d" xlink:href="land-20240630.xsd#land_MetLifeTermLoan2020Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_f1faf39a-6e84-402d-a722-3975fc92a06c" xlink:to="loc_land_MetLifeTermLoan2020Member_3bc49943-e4a3-4d37-8279-c385dbc8dd5d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_MetlifeFacilityMember_64688c68-8982-45ee-834f-d8e1ca891efc" xlink:href="land-20240630.xsd#land_MetlifeFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_f1faf39a-6e84-402d-a722-3975fc92a06c" xlink:to="loc_land_MetlifeFacilityMember_64688c68-8982-45ee-834f-d8e1ca891efc" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_FarmerMacBondsPayableMember_9db19f9f-72f6-4c9f-a6ef-8759be47bf0b" xlink:href="land-20240630.xsd#land_FarmerMacBondsPayableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_f1faf39a-6e84-402d-a722-3975fc92a06c" xlink:to="loc_land_FarmerMacBondsPayableMember_9db19f9f-72f6-4c9f-a6ef-8759be47bf0b" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_LongTermMortgageNotesAndBondsPayableMember_6b6cd031-c0bb-4afb-b2f7-f6bc4af8a516" xlink:href="land-20240630.xsd#land_LongTermMortgageNotesAndBondsPayableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_f1faf39a-6e84-402d-a722-3975fc92a06c" xlink:to="loc_land_LongTermMortgageNotesAndBondsPayableMember_6b6cd031-c0bb-4afb-b2f7-f6bc4af8a516" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_ShortTermMortgageNotesAndBondsPayableMember_583217c6-45b6-49b5-b37d-4167e8f743b4" xlink:href="land-20240630.xsd#land_ShortTermMortgageNotesAndBondsPayableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_f1faf39a-6e84-402d-a722-3975fc92a06c" xlink:to="loc_land_ShortTermMortgageNotesAndBondsPayableMember_583217c6-45b6-49b5-b37d-4167e8f743b4" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_7594659d-8a6d-44d8-a3aa-ea2ce488259b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_e9678a26-7a02-4851-accd-34ea0a5b905f" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_7594659d-8a6d-44d8-a3aa-ea2ce488259b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_7594659d-8a6d-44d8-a3aa-ea2ce488259b_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_7594659d-8a6d-44d8-a3aa-ea2ce488259b" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_7594659d-8a6d-44d8-a3aa-ea2ce488259b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_4716e7b7-0da3-4e42-92a3-11ccdc952907" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_7594659d-8a6d-44d8-a3aa-ea2ce488259b" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_4716e7b7-0da3-4e42-92a3-11ccdc952907" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NotesPayableToBanksMember_4a29dbe6-b9e3-407c-b1e8-8acbe670d743" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NotesPayableToBanksMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_4716e7b7-0da3-4e42-92a3-11ccdc952907" xlink:to="loc_us-gaap_NotesPayableToBanksMember_4a29dbe6-b9e3-407c-b1e8-8acbe670d743" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditMember_a2e4b181-f99f-421f-825e-4618d13026b9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LineOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_4716e7b7-0da3-4e42-92a3-11ccdc952907" xlink:to="loc_us-gaap_LineOfCreditMember_a2e4b181-f99f-421f-825e-4618d13026b9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_a5f2cc44-74ad-465e-a0db-a527286cd4e1" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_e9678a26-7a02-4851-accd-34ea0a5b905f" xlink:to="loc_srt_RangeAxis_a5f2cc44-74ad-465e-a0db-a527286cd4e1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_a5f2cc44-74ad-465e-a0db-a527286cd4e1_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_a5f2cc44-74ad-465e-a0db-a527286cd4e1" xlink:to="loc_srt_RangeMember_a5f2cc44-74ad-465e-a0db-a527286cd4e1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_a8f5ae1c-0ddd-4b5d-839d-9cb3be079f2f" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_a5f2cc44-74ad-465e-a0db-a527286cd4e1" xlink:to="loc_srt_RangeMember_a8f5ae1c-0ddd-4b5d-839d-9cb3be079f2f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_WeightedAverageMember_e11fba9d-089a-48b4-8829-461be2776f6c" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_WeightedAverageMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_a8f5ae1c-0ddd-4b5d-839d-9cb3be079f2f" xlink:to="loc_srt_WeightedAverageMember_e11fba9d-089a-48b4-8829-461be2776f6c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_SecScheduleIiiRealEstateInvestmentPropertyIncludingIntangibleAssetsNet_f8e0217f-75a0-4451-887f-9199ee7e0d97" xlink:href="land-20240630.xsd#land_SecScheduleIiiRealEstateInvestmentPropertyIncludingIntangibleAssetsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e7954b5b-76ed-4d04-8467-222960b57448" xlink:to="loc_land_SecScheduleIiiRealEstateInvestmentPropertyIncludingIntangibleAssetsNet_f8e0217f-75a0-4451-887f-9199ee7e0d97" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_DebtInstrumentWeightedAverageInterestRate_66d5a445-c57b-4372-ad54-c162ce6bc9b1" xlink:href="land-20240630.xsd#land_DebtInstrumentWeightedAverageInterestRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e7954b5b-76ed-4d04-8467-222960b57448" xlink:to="loc_land_DebtInstrumentWeightedAverageInterestRate_66d5a445-c57b-4372-ad54-c162ce6bc9b1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateIncreaseDecrease_4f8b018e-76a4-460c-b1ba-d7dfc29b0784" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentInterestRateIncreaseDecrease"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e7954b5b-76ed-4d04-8467-222960b57448" xlink:to="loc_us-gaap_DebtInstrumentInterestRateIncreaseDecrease_4f8b018e-76a4-460c-b1ba-d7dfc29b0784" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_DebtInstrumentBasisSpreadOnVariableRateIncreaseDecrease_03685f0c-297d-47f8-9fd0-f5d4707ae216" xlink:href="land-20240630.xsd#land_DebtInstrumentBasisSpreadOnVariableRateIncreaseDecrease"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e7954b5b-76ed-4d04-8467-222960b57448" xlink:to="loc_land_DebtInstrumentBasisSpreadOnVariableRateIncreaseDecrease_03685f0c-297d-47f8-9fd0-f5d4707ae216" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentFaceAmount_3c5b922c-c86b-4ff2-b2e2-1896a73f7ce2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentFaceAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e7954b5b-76ed-4d04-8467-222960b57448" xlink:to="loc_us-gaap_DebtInstrumentFaceAmount_3c5b922c-c86b-4ff2-b2e2-1896a73f7ce2" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTerm_8d950012-b5ad-49cc-9660-a5ee073bfa9c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e7954b5b-76ed-4d04-8467-222960b57448" xlink:to="loc_us-gaap_DebtInstrumentTerm_8d950012-b5ad-49cc-9660-a5ee073bfa9c" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuredDebt_867a0037-9941-47d9-8cf0-d448910be2cc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SecuredDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e7954b5b-76ed-4d04-8467-222960b57448" xlink:to="loc_us-gaap_SecuredDebt_867a0037-9941-47d9-8cf0-d448910be2cc" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNonoperatingIncome_c2a8ca6b-5589-499d-aa7c-cfcef767057d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherNonoperatingIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e7954b5b-76ed-4d04-8467-222960b57448" xlink:to="loc_us-gaap_OtherNonoperatingIncome_c2a8ca6b-5589-499d-aa7c-cfcef767057d" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RepaymentsOfLongTermDebt_a5e18734-43b6-41b1-817b-c1f376277d58" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RepaymentsOfLongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e7954b5b-76ed-4d04-8467-222960b57448" xlink:to="loc_us-gaap_RepaymentsOfLongTermDebt_a5e18734-43b6-41b1-817b-c1f376277d58" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_e70933b9-e294-4182-a4da-8fcf4f8d0a1b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentInterestRateStatedPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e7954b5b-76ed-4d04-8467-222960b57448" xlink:to="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_e70933b9-e294-4182-a4da-8fcf4f8d0a1b" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtFairValue_49cfff83-671d-4dae-b25d-5fb68d9fea03" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongTermDebtFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e7954b5b-76ed-4d04-8467-222960b57448" xlink:to="loc_us-gaap_LongTermDebtFairValue_49cfff83-671d-4dae-b25d-5fb68d9fea03" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCredit_30c06b0e-dea4-4285-a752-7bdb46c11052" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LineOfCredit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e7954b5b-76ed-4d04-8467-222960b57448" xlink:to="loc_us-gaap_LineOfCredit_30c06b0e-dea4-4285-a752-7bdb46c11052" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashFlowHedgeGainLossToBeReclassifiedWithinTwelveMonths_9d99b4cf-184e-4601-b19c-2f966952d1a1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashFlowHedgeGainLossToBeReclassifiedWithinTwelveMonths"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_e7954b5b-76ed-4d04-8467-222960b57448" xlink:to="loc_us-gaap_CashFlowHedgeGainLossToBeReclassifiedWithinTwelveMonths_9d99b4cf-184e-4601-b19c-2f966952d1a1" xlink:type="arc" order="12"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.gladstoneland.com/role/BorrowingsScheduleofBorrowingsbyTypeDetails" xlink:type="simple" xlink:href="land-20240630.xsd#BorrowingsScheduleofBorrowingsbyTypeDetails"/>
  <link:definitionLink xlink:role="http://www.gladstoneland.com/role/BorrowingsScheduleofBorrowingsbyTypeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_ddd51c02-975a-4a4c-bd42-9fd9c973575c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_f4ec977e-4b39-462e-a1b4-43661874d9eb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_ddd51c02-975a-4a4c-bd42-9fd9c973575c" xlink:to="loc_us-gaap_DebtInstrumentTable_f4ec977e-4b39-462e-a1b4-43661874d9eb" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_95e74aad-4839-4657-a838-09b9b0c53cc4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_f4ec977e-4b39-462e-a1b4-43661874d9eb" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_95e74aad-4839-4657-a838-09b9b0c53cc4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_95e74aad-4839-4657-a838-09b9b0c53cc4_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_95e74aad-4839-4657-a838-09b9b0c53cc4" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_95e74aad-4839-4657-a838-09b9b0c53cc4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_9b556ac2-26c7-4d02-86b3-f52ada04ad4e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_95e74aad-4839-4657-a838-09b9b0c53cc4" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_9b556ac2-26c7-4d02-86b3-f52ada04ad4e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditMember_e2cec240-ac0e-48ca-98ee-bcf632ef6a04" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LineOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_9b556ac2-26c7-4d02-86b3-f52ada04ad4e" xlink:to="loc_us-gaap_LineOfCreditMember_e2cec240-ac0e-48ca-98ee-bcf632ef6a04" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NotesPayableToBanksMember_f268c381-7ed6-46dc-ad0c-99b7f1b15fe8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NotesPayableToBanksMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_9b556ac2-26c7-4d02-86b3-f52ada04ad4e" xlink:to="loc_us-gaap_NotesPayableToBanksMember_f268c381-7ed6-46dc-ad0c-99b7f1b15fe8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_3ee38236-0595-427a-ad6a-bcca829e6f20" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_f4ec977e-4b39-462e-a1b4-43661874d9eb" xlink:to="loc_srt_RangeAxis_3ee38236-0595-427a-ad6a-bcca829e6f20" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_3ee38236-0595-427a-ad6a-bcca829e6f20_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_3ee38236-0595-427a-ad6a-bcca829e6f20" xlink:to="loc_srt_RangeMember_3ee38236-0595-427a-ad6a-bcca829e6f20_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_fc8de91c-436c-4996-94f7-7a7e1b79a061" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_3ee38236-0595-427a-ad6a-bcca829e6f20" xlink:to="loc_srt_RangeMember_fc8de91c-436c-4996-94f7-7a7e1b79a061" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_29e75b12-0854-479c-8fbb-bbea13cf32d5" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_fc8de91c-436c-4996-94f7-7a7e1b79a061" xlink:to="loc_srt_MinimumMember_29e75b12-0854-479c-8fbb-bbea13cf32d5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_d4978170-1682-4cb5-9af3-a305933ac895" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_fc8de91c-436c-4996-94f7-7a7e1b79a061" xlink:to="loc_srt_MaximumMember_d4978170-1682-4cb5-9af3-a305933ac895" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_70f00b84-1648-4a21-9f9f-bc271dd00486" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_f4ec977e-4b39-462e-a1b4-43661874d9eb" xlink:to="loc_us-gaap_DebtInstrumentAxis_70f00b84-1648-4a21-9f9f-bc271dd00486" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_70f00b84-1648-4a21-9f9f-bc271dd00486_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_70f00b84-1648-4a21-9f9f-bc271dd00486" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_70f00b84-1648-4a21-9f9f-bc271dd00486_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_8e1ed028-04b3-473b-8403-db3cb3f0eaec" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_70f00b84-1648-4a21-9f9f-bc271dd00486" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_8e1ed028-04b3-473b-8403-db3cb3f0eaec" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_MetLifeMember_f59746f8-4e33-46a2-ba0e-6b0390445187" xlink:href="land-20240630.xsd#land_MetLifeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_8e1ed028-04b3-473b-8403-db3cb3f0eaec" xlink:to="loc_land_MetLifeMember_f59746f8-4e33-46a2-ba0e-6b0390445187" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_MetlifeFacilityMember_51974ba2-fd97-4a3c-bf88-282131c8d0f3" xlink:href="land-20240630.xsd#land_MetlifeFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_land_MetLifeMember_f59746f8-4e33-46a2-ba0e-6b0390445187" xlink:to="loc_land_MetlifeFacilityMember_51974ba2-fd97-4a3c-bf88-282131c8d0f3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_MetLifeTermLoan2020Member_db65d70e-2bdc-47ef-be65-2c87d1aad511" xlink:href="land-20240630.xsd#land_MetLifeTermLoan2020Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_land_MetLifeMember_f59746f8-4e33-46a2-ba0e-6b0390445187" xlink:to="loc_land_MetLifeTermLoan2020Member_db65d70e-2bdc-47ef-be65-2c87d1aad511" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_MetLifeTermLoan2022Member_25baaecf-8028-4148-9623-8b572371ff83" xlink:href="land-20240630.xsd#land_MetLifeTermLoan2022Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_land_MetLifeMember_f59746f8-4e33-46a2-ba0e-6b0390445187" xlink:to="loc_land_MetLifeTermLoan2022Member_25baaecf-8028-4148-9623-8b572371ff83" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentFaceAmount_beebd959-2ddd-4b78-9fb1-5c7e7030f3e0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentFaceAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_ddd51c02-975a-4a4c-bd42-9fd9c973575c" xlink:to="loc_us-gaap_DebtInstrumentFaceAmount_beebd959-2ddd-4b78-9fb1-5c7e7030f3e0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentCarryingAmount_c536f474-f094-49c1-8c4c-ff1586d5eb33" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentCarryingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_ddd51c02-975a-4a4c-bd42-9fd9c973575c" xlink:to="loc_us-gaap_DebtInstrumentCarryingAmount_c536f474-f094-49c1-8c4c-ff1586d5eb33" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_4d491cc6-1367-4b22-a95f-f5941f604ccc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentBasisSpreadOnVariableRate1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_ddd51c02-975a-4a4c-bd42-9fd9c973575c" xlink:to="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_4d491cc6-1367-4b22-a95f-f5941f604ccc" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_ea9ee614-d5c6-4edd-9c2e-a7a6deed5c89" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentInterestRateStatedPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_ddd51c02-975a-4a4c-bd42-9fd9c973575c" xlink:to="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_ea9ee614-d5c6-4edd-9c2e-a7a6deed5c89" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentUnusedBorrowingCapacityAmount_295855a7-bd86-4a2d-b936-5cdb7b354dee" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentUnusedBorrowingCapacityAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_ddd51c02-975a-4a4c-bd42-9fd9c973575c" xlink:to="loc_us-gaap_DebtInstrumentUnusedBorrowingCapacityAmount_295855a7-bd86-4a2d-b936-5cdb7b354dee" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_LongTermDebtAdditionalAvailableAmount_d318e7fa-1531-4999-9bb6-4f1dd109dab9" xlink:href="land-20240630.xsd#land_LongTermDebtAdditionalAvailableAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_ddd51c02-975a-4a4c-bd42-9fd9c973575c" xlink:to="loc_land_LongTermDebtAdditionalAvailableAmount_d318e7fa-1531-4999-9bb6-4f1dd109dab9" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_DebtInstrumentMinimumAnnualizedRate_22d6234c-9eab-4c17-bb1c-e42c568d637b" xlink:href="land-20240630.xsd#land_DebtInstrumentMinimumAnnualizedRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_ddd51c02-975a-4a4c-bd42-9fd9c973575c" xlink:to="loc_land_DebtInstrumentMinimumAnnualizedRate_22d6234c-9eab-4c17-bb1c-e42c568d637b" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityUnusedCapacityCommitmentFeePercentage_dbbb580a-949d-4a39-aabf-4b3c67d00ec6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LineOfCreditFacilityUnusedCapacityCommitmentFeePercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_ddd51c02-975a-4a4c-bd42-9fd9c973575c" xlink:to="loc_us-gaap_LineOfCreditFacilityUnusedCapacityCommitmentFeePercentage_dbbb580a-949d-4a39-aabf-4b3c67d00ec6" xlink:type="arc" order="7"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.gladstoneland.com/role/BorrowingsAggregateMaturitiesDetails" xlink:type="simple" xlink:href="land-20240630.xsd#BorrowingsAggregateMaturitiesDetails"/>
  <link:definitionLink xlink:role="http://www.gladstoneland.com/role/BorrowingsAggregateMaturitiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_8513a750-dc93-4d74-b73c-63ac7025d00e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_b648402b-10f9-4ecf-95bd-66eb35bb57b4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_8513a750-dc93-4d74-b73c-63ac7025d00e" xlink:to="loc_us-gaap_DebtInstrumentTable_b648402b-10f9-4ecf-95bd-66eb35bb57b4" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_ba455440-65fd-4bea-8024-53a435620ad8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_b648402b-10f9-4ecf-95bd-66eb35bb57b4" xlink:to="loc_us-gaap_DebtInstrumentAxis_ba455440-65fd-4bea-8024-53a435620ad8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_ba455440-65fd-4bea-8024-53a435620ad8_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_ba455440-65fd-4bea-8024-53a435620ad8" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_ba455440-65fd-4bea-8024-53a435620ad8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_fe6b40ad-f407-4644-8fa4-cd319d389bc2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_ba455440-65fd-4bea-8024-53a435620ad8" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_fe6b40ad-f407-4644-8fa4-cd319d389bc2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_LongTermMortgageNotesAndBondsPayableMember_07669e75-2c3c-4229-8115-2cc738ae5105" xlink:href="land-20240630.xsd#land_LongTermMortgageNotesAndBondsPayableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_fe6b40ad-f407-4644-8fa4-cd319d389bc2" xlink:to="loc_land_LongTermMortgageNotesAndBondsPayableMember_07669e75-2c3c-4229-8115-2cc738ae5105" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalRemainderOfFiscalYear_d7096ca9-30fd-4389-bb80-9713d1e484c4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalRemainderOfFiscalYear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_8513a750-dc93-4d74-b73c-63ac7025d00e" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalRemainderOfFiscalYear_d7096ca9-30fd-4389-bb80-9713d1e484c4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths_8591bd33-8fb6-46b8-a1fa-bb3cbfcbbcbd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_8513a750-dc93-4d74-b73c-63ac7025d00e" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths_8591bd33-8fb6-46b8-a1fa-bb3cbfcbbcbd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo_11a16e40-18d3-490e-89a5-e2b935fad189" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_8513a750-dc93-4d74-b73c-63ac7025d00e" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo_11a16e40-18d3-490e-89a5-e2b935fad189" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree_99ded3d1-2aa6-4371-815a-17f974254d4e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_8513a750-dc93-4d74-b73c-63ac7025d00e" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree_99ded3d1-2aa6-4371-815a-17f974254d4e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearFour_b9c8247b-e204-4b54-964d-4580cc8f59d8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearFour"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_8513a750-dc93-4d74-b73c-63ac7025d00e" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearFour_b9c8247b-e204-4b54-964d-4580cc8f59d8" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearFive_81a70ae7-d6c9-4115-8738-cdf01dc4e77f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearFive"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_8513a750-dc93-4d74-b73c-63ac7025d00e" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearFive_81a70ae7-d6c9-4115-8738-cdf01dc4e77f" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalAfterYearFive_374f5596-d750-43b6-8e74-7484ac48c1ea" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalAfterYearFive"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_8513a750-dc93-4d74-b73c-63ac7025d00e" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalAfterYearFive_374f5596-d750-43b6-8e74-7484ac48c1ea" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentCarryingAmount_e6240e1c-8fdc-44b1-a59d-5198ea529ab0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentCarryingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_8513a750-dc93-4d74-b73c-63ac7025d00e" xlink:to="loc_us-gaap_DebtInstrumentCarryingAmount_e6240e1c-8fdc-44b1-a59d-5198ea529ab0" xlink:type="arc" order="7"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.gladstoneland.com/role/BorrowingsInterestRateSwapAgreementDetails" xlink:type="simple" xlink:href="land-20240630.xsd#BorrowingsInterestRateSwapAgreementDetails"/>
  <link:definitionLink xlink:role="http://www.gladstoneland.com/role/BorrowingsInterestRateSwapAgreementDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_33ede4fc-7f47-4d18-92c7-94bc18e20de9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_eab89ba8-be94-465d-8b47-e03fc315b720" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_33ede4fc-7f47-4d18-92c7-94bc18e20de9" xlink:to="loc_us-gaap_DebtInstrumentTable_eab89ba8-be94-465d-8b47-e03fc315b720" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeInstrumentRiskAxis_c52ebe2b-9cfa-48e2-9afc-108f7091e590" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DerivativeInstrumentRiskAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_eab89ba8-be94-465d-8b47-e03fc315b720" xlink:to="loc_us-gaap_DerivativeInstrumentRiskAxis_c52ebe2b-9cfa-48e2-9afc-108f7091e590" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_c52ebe2b-9cfa-48e2-9afc-108f7091e590_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_c52ebe2b-9cfa-48e2-9afc-108f7091e590" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_c52ebe2b-9cfa-48e2-9afc-108f7091e590_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_f1ba7ca1-1927-4325-ac7e-81a6ef20b911" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_c52ebe2b-9cfa-48e2-9afc-108f7091e590" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_f1ba7ca1-1927-4325-ac7e-81a6ef20b911" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestRateSwapMember_5d8a3234-a2d4-4741-bdc9-fe80427e91f6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InterestRateSwapMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeContractTypeDomain_f1ba7ca1-1927-4325-ac7e-81a6ef20b911" xlink:to="loc_us-gaap_InterestRateSwapMember_5d8a3234-a2d4-4741-bdc9-fe80427e91f6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_NumberOfDerivativeInstruments_5ef42f81-4be3-4969-9f52-275796d1ea51" xlink:href="land-20240630.xsd#land_NumberOfDerivativeInstruments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_33ede4fc-7f47-4d18-92c7-94bc18e20de9" xlink:to="loc_land_NumberOfDerivativeInstruments_5ef42f81-4be3-4969-9f52-275796d1ea51" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeNotionalAmount_a9e5d110-60d1-4b5f-b56e-ca0be7f81350" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DerivativeNotionalAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_33ede4fc-7f47-4d18-92c7-94bc18e20de9" xlink:to="loc_us-gaap_DerivativeNotionalAmount_a9e5d110-60d1-4b5f-b56e-ca0be7f81350" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeAssetNotionalAmount_5866dc0d-01a3-4a74-9816-1e6ee3d7d379" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DerivativeAssetNotionalAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_33ede4fc-7f47-4d18-92c7-94bc18e20de9" xlink:to="loc_us-gaap_DerivativeAssetNotionalAmount_5866dc0d-01a3-4a74-9816-1e6ee3d7d379" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_2531ce4a-bf70-4bbd-9dc0-b563398b66fe" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_33ede4fc-7f47-4d18-92c7-94bc18e20de9" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_2531ce4a-bf70-4bbd-9dc0-b563398b66fe" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInFairValueOfInterestRateFairValueHedgingInstruments1_50329bc4-7f70-49d8-809e-48aa68d2b73c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInFairValueOfInterestRateFairValueHedgingInstruments1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_33ede4fc-7f47-4d18-92c7-94bc18e20de9" xlink:to="loc_us-gaap_IncreaseDecreaseInFairValueOfInterestRateFairValueHedgingInstruments1_50329bc4-7f70-49d8-809e-48aa68d2b73c" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.gladstoneland.com/role/CumulativeTermPreferredStockDetails" xlink:type="simple" xlink:href="land-20240630.xsd#CumulativeTermPreferredStockDetails"/>
  <link:definitionLink xlink:role="http://www.gladstoneland.com/role/CumulativeTermPreferredStockDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsidiarySaleOfStockLineItems_9fa88d3e-412c-48a9-be0e-26206b9fb552" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsidiarySaleOfStockLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsidiaryOrEquityMethodInvesteeSaleOfStockBySubsidiaryOrEquityInvesteeTable_e6653f13-d4ce-49df-a67c-89d69b00000e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsidiaryOrEquityMethodInvesteeSaleOfStockBySubsidiaryOrEquityInvesteeTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_9fa88d3e-412c-48a9-be0e-26206b9fb552" xlink:to="loc_us-gaap_SubsidiaryOrEquityMethodInvesteeSaleOfStockBySubsidiaryOrEquityInvesteeTable_e6653f13-d4ce-49df-a67c-89d69b00000e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_1376100b-0911-47cb-a105-d8ee150db883" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsidiaryOrEquityMethodInvesteeSaleOfStockBySubsidiaryOrEquityInvesteeTable_e6653f13-d4ce-49df-a67c-89d69b00000e" xlink:to="loc_us-gaap_StatementClassOfStockAxis_1376100b-0911-47cb-a105-d8ee150db883" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_1376100b-0911-47cb-a105-d8ee150db883_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_1376100b-0911-47cb-a105-d8ee150db883" xlink:to="loc_us-gaap_ClassOfStockDomain_1376100b-0911-47cb-a105-d8ee150db883_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_28bf0663-9038-47ba-9a39-0c95c7f7706c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_1376100b-0911-47cb-a105-d8ee150db883" xlink:to="loc_us-gaap_ClassOfStockDomain_28bf0663-9038-47ba-9a39-0c95c7f7706c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesDPreferredStockMember_0769355b-dfa2-4438-b86e-c87d7f45e69a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesDPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_28bf0663-9038-47ba-9a39-0c95c7f7706c" xlink:to="loc_us-gaap_SeriesDPreferredStockMember_0769355b-dfa2-4438-b86e-c87d7f45e69a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_03b8b95e-8a75-4f30-8cf7-a2228356c07e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsidiaryOrEquityMethodInvesteeSaleOfStockBySubsidiaryOrEquityInvesteeTable_e6653f13-d4ce-49df-a67c-89d69b00000e" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_03b8b95e-8a75-4f30-8cf7-a2228356c07e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_03b8b95e-8a75-4f30-8cf7-a2228356c07e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_03b8b95e-8a75-4f30-8cf7-a2228356c07e" xlink:to="loc_us-gaap_EquityComponentDomain_03b8b95e-8a75-4f30-8cf7-a2228356c07e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_740dd4b8-ab10-476f-9abf-d434a17342d9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_03b8b95e-8a75-4f30-8cf7-a2228356c07e" xlink:to="loc_us-gaap_EquityComponentDomain_740dd4b8-ab10-476f-9abf-d434a17342d9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockMember_9a820e6b-e97b-4fd3-b2a3-cef1c9c0792d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_740dd4b8-ab10-476f-9abf-d434a17342d9" xlink:to="loc_us-gaap_PreferredStockMember_9a820e6b-e97b-4fd3-b2a3-cef1c9c0792d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_c5032b9a-ee07-4265-9d73-c9d2331b631a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsidiaryOrEquityMethodInvesteeSaleOfStockBySubsidiaryOrEquityInvesteeTable_e6653f13-d4ce-49df-a67c-89d69b00000e" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_c5032b9a-ee07-4265-9d73-c9d2331b631a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_c5032b9a-ee07-4265-9d73-c9d2331b631a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_c5032b9a-ee07-4265-9d73-c9d2331b631a" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_c5032b9a-ee07-4265-9d73-c9d2331b631a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_3d42e054-51c1-423a-b516-54279319f0fa" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_c5032b9a-ee07-4265-9d73-c9d2331b631a" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_3d42e054-51c1-423a-b516-54279319f0fa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_9f9888d0-9a6b-4dbb-b8bd-7925a2dc1bc3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_3d42e054-51c1-423a-b516-54279319f0fa" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_9f9888d0-9a6b-4dbb-b8bd-7925a2dc1bc3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockDividendRatePercentage_25be70eb-4a20-460f-a3d8-4b432c927125" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockDividendRatePercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_9fa88d3e-412c-48a9-be0e-26206b9fb552" xlink:to="loc_us-gaap_PreferredStockDividendRatePercentage_25be70eb-4a20-460f-a3d8-4b432c927125" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockParOrStatedValuePerShare_99199427-a13f-45c6-a554-cb23dad86b65" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockParOrStatedValuePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_9fa88d3e-412c-48a9-be0e-26206b9fb552" xlink:to="loc_us-gaap_PreferredStockParOrStatedValuePerShare_99199427-a13f-45c6-a554-cb23dad86b65" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharesIssuedPricePerShare_ba9ead18-3324-43f8-9778-c1ec148f0ede" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SharesIssuedPricePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_9fa88d3e-412c-48a9-be0e-26206b9fb552" xlink:to="loc_us-gaap_SharesIssuedPricePerShare_ba9ead18-3324-43f8-9778-c1ec148f0ede" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesIssued_1aaa4213-a850-4969-acf9-80a6e1de79ad" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_9fa88d3e-412c-48a9-be0e-26206b9fb552" xlink:to="loc_us-gaap_PreferredStockSharesIssued_1aaa4213-a850-4969-acf9-80a6e1de79ad" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOfPreferredStockAndPreferenceStock_2b9783ea-b84b-4516-b0fc-784e7fdb811f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromIssuanceOfPreferredStockAndPreferenceStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_9fa88d3e-412c-48a9-be0e-26206b9fb552" xlink:to="loc_us-gaap_ProceedsFromIssuanceOfPreferredStockAndPreferenceStock_2b9783ea-b84b-4516-b0fc-784e7fdb811f" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOrSaleOfEquity_779783cb-9a82-4845-85af-9496983a2259" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromIssuanceOrSaleOfEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_9fa88d3e-412c-48a9-be0e-26206b9fb552" xlink:to="loc_us-gaap_ProceedsFromIssuanceOrSaleOfEquity_779783cb-9a82-4845-85af-9496983a2259" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockRedemptionPricePerShare_015a8c1e-9f73-4a22-b8e3-b1c3f93308f2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockRedemptionPricePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_9fa88d3e-412c-48a9-be0e-26206b9fb552" xlink:to="loc_us-gaap_PreferredStockRedemptionPricePerShare_015a8c1e-9f73-4a22-b8e3-b1c3f93308f2" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsOfStockIssuanceCosts_885c1e4c-593e-4da7-af0a-a40bb8fcee3a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PaymentsOfStockIssuanceCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_9fa88d3e-412c-48a9-be0e-26206b9fb552" xlink:to="loc_us-gaap_PaymentsOfStockIssuanceCosts_885c1e4c-593e-4da7-af0a-a40bb8fcee3a" xlink:type="arc" order="7"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.gladstoneland.com/role/RelatedPartyTransactionsAdditionalInformationDetails" xlink:type="simple" xlink:href="land-20240630.xsd#RelatedPartyTransactionsAdditionalInformationDetails"/>
  <link:definitionLink xlink:role="http://www.gladstoneland.com/role/RelatedPartyTransactionsAdditionalInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionLineItems_4f6826c1-c69d-48f3-a099-c0943068d626" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyTransactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_c67c06ab-acb4-452a-85ec-3fff612f5079" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_4f6826c1-c69d-48f3-a099-c0943068d626" xlink:to="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_c67c06ab-acb4-452a-85ec-3fff612f5079" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionAxis_bc071479-dfd8-4b9a-8703-a3455a2ff802" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyTransactionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_c67c06ab-acb4-452a-85ec-3fff612f5079" xlink:to="loc_us-gaap_RelatedPartyTransactionAxis_bc071479-dfd8-4b9a-8703-a3455a2ff802" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionDomain_bc071479-dfd8-4b9a-8703-a3455a2ff802_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RelatedPartyTransactionAxis_bc071479-dfd8-4b9a-8703-a3455a2ff802" xlink:to="loc_us-gaap_RelatedPartyTransactionDomain_bc071479-dfd8-4b9a-8703-a3455a2ff802_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionDomain_cd9dcbb8-42ff-4b94-8e23-ca587607b04f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RelatedPartyTransactionAxis_bc071479-dfd8-4b9a-8703-a3455a2ff802" xlink:to="loc_us-gaap_RelatedPartyTransactionDomain_cd9dcbb8-42ff-4b94-8e23-ca587607b04f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_AdvisoryAgreementBaseRateAnnualizedRateMember_5fc9adca-1d37-40f0-8573-8104eef835cb" xlink:href="land-20240630.xsd#land_AdvisoryAgreementBaseRateAnnualizedRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_cd9dcbb8-42ff-4b94-8e23-ca587607b04f" xlink:to="loc_land_AdvisoryAgreementBaseRateAnnualizedRateMember_5fc9adca-1d37-40f0-8573-8104eef835cb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_AdvisoryAgreementBaseRateQuarterlyRateMember_b17ccf51-c802-419a-b8b2-eb5bf999e762" xlink:href="land-20240630.xsd#land_AdvisoryAgreementBaseRateQuarterlyRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_cd9dcbb8-42ff-4b94-8e23-ca587607b04f" xlink:to="loc_land_AdvisoryAgreementBaseRateQuarterlyRateMember_b17ccf51-c802-419a-b8b2-eb5bf999e762" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_AdvisoryAgreementIncentiveRateQuarterlyHurdleRateMember_ddedcde2-a03e-4aad-b6c4-a653321cc9f2" xlink:href="land-20240630.xsd#land_AdvisoryAgreementIncentiveRateQuarterlyHurdleRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_cd9dcbb8-42ff-4b94-8e23-ca587607b04f" xlink:to="loc_land_AdvisoryAgreementIncentiveRateQuarterlyHurdleRateMember_ddedcde2-a03e-4aad-b6c4-a653321cc9f2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_AdvisoryAgreementIncentiveRateAnnualizedHurdleRateMember_c167fe22-617d-4370-a395-f85048b7944f" xlink:href="land-20240630.xsd#land_AdvisoryAgreementIncentiveRateAnnualizedHurdleRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_cd9dcbb8-42ff-4b94-8e23-ca587607b04f" xlink:to="loc_land_AdvisoryAgreementIncentiveRateAnnualizedHurdleRateMember_c167fe22-617d-4370-a395-f85048b7944f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_AdvisoryAgreementIncentiveRatePreIncentiveFeeNetInvestmentIncomeBelowCatchUpThresholdMember_ccc8852f-2e53-4127-b827-446e6d1b677b" xlink:href="land-20240630.xsd#land_AdvisoryAgreementIncentiveRatePreIncentiveFeeNetInvestmentIncomeBelowCatchUpThresholdMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_cd9dcbb8-42ff-4b94-8e23-ca587607b04f" xlink:to="loc_land_AdvisoryAgreementIncentiveRatePreIncentiveFeeNetInvestmentIncomeBelowCatchUpThresholdMember_ccc8852f-2e53-4127-b827-446e6d1b677b" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_AdvisoryAgreementIncentiveRateQuarterlyCatchUpThresholdMember_430bcb12-ed2a-4a6c-bcfe-dffa4e8d8fef" xlink:href="land-20240630.xsd#land_AdvisoryAgreementIncentiveRateQuarterlyCatchUpThresholdMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_cd9dcbb8-42ff-4b94-8e23-ca587607b04f" xlink:to="loc_land_AdvisoryAgreementIncentiveRateQuarterlyCatchUpThresholdMember_430bcb12-ed2a-4a6c-bcfe-dffa4e8d8fef" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_AdvisoryAgreementIncentiveRateAnnualizedCatchUpThresholdMember_154de891-2297-4abb-ab72-c591584c7fc2" xlink:href="land-20240630.xsd#land_AdvisoryAgreementIncentiveRateAnnualizedCatchUpThresholdMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_cd9dcbb8-42ff-4b94-8e23-ca587607b04f" xlink:to="loc_land_AdvisoryAgreementIncentiveRateAnnualizedCatchUpThresholdMember_154de891-2297-4abb-ab72-c591584c7fc2" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_AdvisoryAgreementIncentiveRatePreIncentiveFeeNetInvestmentIncomeExceedsCatchUpThresholdMember_32780f7c-e910-4949-8ee0-8556531108fb" xlink:href="land-20240630.xsd#land_AdvisoryAgreementIncentiveRatePreIncentiveFeeNetInvestmentIncomeExceedsCatchUpThresholdMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_cd9dcbb8-42ff-4b94-8e23-ca587607b04f" xlink:to="loc_land_AdvisoryAgreementIncentiveRatePreIncentiveFeeNetInvestmentIncomeExceedsCatchUpThresholdMember_32780f7c-e910-4949-8ee0-8556531108fb" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_AdvisoryAgreementIncentiveRateRealizedCapitalGainsMember_d8d5ed22-b8af-4feb-919b-f20029d42b93" xlink:href="land-20240630.xsd#land_AdvisoryAgreementIncentiveRateRealizedCapitalGainsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_cd9dcbb8-42ff-4b94-8e23-ca587607b04f" xlink:to="loc_land_AdvisoryAgreementIncentiveRateRealizedCapitalGainsMember_d8d5ed22-b8af-4feb-919b-f20029d42b93" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_FinancingArrangementAgreementFinancingFeesMember_362389c1-03fc-40fb-b401-f0214219af34" xlink:href="land-20240630.xsd#land_FinancingArrangementAgreementFinancingFeesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_cd9dcbb8-42ff-4b94-8e23-ca587607b04f" xlink:to="loc_land_FinancingArrangementAgreementFinancingFeesMember_362389c1-03fc-40fb-b401-f0214219af34" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_FinancingArrangementAgreementPercentageOfFinancingObtainedMember_aa19caef-94b4-4d74-8960-9e6def80a651" xlink:href="land-20240630.xsd#land_FinancingArrangementAgreementPercentageOfFinancingObtainedMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_land_FinancingArrangementAgreementFinancingFeesMember_362389c1-03fc-40fb-b401-f0214219af34" xlink:to="loc_land_FinancingArrangementAgreementPercentageOfFinancingObtainedMember_aa19caef-94b4-4d74-8960-9e6def80a651" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_FinancingArrangementAgreementPercentageOfFinancingFeesPaidMember_196dafa0-50ca-4a7a-8e50-91b522f8f8c6" xlink:href="land-20240630.xsd#land_FinancingArrangementAgreementPercentageOfFinancingFeesPaidMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_land_FinancingArrangementAgreementFinancingFeesMember_362389c1-03fc-40fb-b401-f0214219af34" xlink:to="loc_land_FinancingArrangementAgreementPercentageOfFinancingFeesPaidMember_196dafa0-50ca-4a7a-8e50-91b522f8f8c6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_DealerManagerAgreementsSellingCommissionsOfGrossProceedsFromSalesMember_c7088b9a-56b9-4b98-a3ad-7155965c1150" xlink:href="land-20240630.xsd#land_DealerManagerAgreementsSellingCommissionsOfGrossProceedsFromSalesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_cd9dcbb8-42ff-4b94-8e23-ca587607b04f" xlink:to="loc_land_DealerManagerAgreementsSellingCommissionsOfGrossProceedsFromSalesMember_c7088b9a-56b9-4b98-a3ad-7155965c1150" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_DealerManagerAgreementsDealerManagerFeeOfGrossProceedsFromSalesMember_0941a931-617e-40c7-af8a-1b5eaa02c821" xlink:href="land-20240630.xsd#land_DealerManagerAgreementsDealerManagerFeeOfGrossProceedsFromSalesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_cd9dcbb8-42ff-4b94-8e23-ca587607b04f" xlink:to="loc_land_DealerManagerAgreementsDealerManagerFeeOfGrossProceedsFromSalesMember_0941a931-617e-40c7-af8a-1b5eaa02c821" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_f4edd735-0d37-4874-90d7-cc4561f8a8fc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyTransactionsByRelatedPartyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_c67c06ab-acb4-452a-85ec-3fff612f5079" xlink:to="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_f4edd735-0d37-4874-90d7-cc4561f8a8fc" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_f4edd735-0d37-4874-90d7-cc4561f8a8fc_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_f4edd735-0d37-4874-90d7-cc4561f8a8fc" xlink:to="loc_us-gaap_RelatedPartyDomain_f4edd735-0d37-4874-90d7-cc4561f8a8fc_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_22e8d4db-a7e7-402a-9aac-823dd8530f1b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_f4edd735-0d37-4874-90d7-cc4561f8a8fc" xlink:to="loc_us-gaap_RelatedPartyDomain_22e8d4db-a7e7-402a-9aac-823dd8530f1b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_AffiliatedEntityMember_94cb9a18-7a9c-4036-ad63-999aaff67148" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_AffiliatedEntityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyDomain_22e8d4db-a7e7-402a-9aac-823dd8530f1b" xlink:to="loc_srt_AffiliatedEntityMember_94cb9a18-7a9c-4036-ad63-999aaff67148" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_d140942e-9b8d-4c99-8d66-6b92029bd521" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_c67c06ab-acb4-452a-85ec-3fff612f5079" xlink:to="loc_srt_RangeAxis_d140942e-9b8d-4c99-8d66-6b92029bd521" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_d140942e-9b8d-4c99-8d66-6b92029bd521_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_d140942e-9b8d-4c99-8d66-6b92029bd521" xlink:to="loc_srt_RangeMember_d140942e-9b8d-4c99-8d66-6b92029bd521_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_b54f8b09-ad04-46c0-a29f-440577a1f717" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_d140942e-9b8d-4c99-8d66-6b92029bd521" xlink:to="loc_srt_RangeMember_b54f8b09-ad04-46c0-a29f-440577a1f717" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_fb1c435d-b26f-4d83-bfb3-57a1e3ad1753" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_b54f8b09-ad04-46c0-a29f-440577a1f717" xlink:to="loc_srt_MinimumMember_fb1c435d-b26f-4d83-bfb3-57a1e3ad1753" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_2946dd2e-ffec-4680-9d90-b77447ca694d" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_b54f8b09-ad04-46c0-a29f-440577a1f717" xlink:to="loc_srt_MaximumMember_2946dd2e-ffec-4680-9d90-b77447ca694d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_4bf20d89-689b-45dd-9810-b7ea6251c09a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_c67c06ab-acb4-452a-85ec-3fff612f5079" xlink:to="loc_us-gaap_StatementClassOfStockAxis_4bf20d89-689b-45dd-9810-b7ea6251c09a" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_4bf20d89-689b-45dd-9810-b7ea6251c09a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_4bf20d89-689b-45dd-9810-b7ea6251c09a" xlink:to="loc_us-gaap_ClassOfStockDomain_4bf20d89-689b-45dd-9810-b7ea6251c09a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_684ec5dc-8cbf-4143-817e-966cb7260151" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_4bf20d89-689b-45dd-9810-b7ea6251c09a" xlink:to="loc_us-gaap_ClassOfStockDomain_684ec5dc-8cbf-4143-817e-966cb7260151" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesEPreferredStockMember_150df131-30d7-422c-bf0f-8c731197e6b5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesEPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_684ec5dc-8cbf-4143-817e-966cb7260151" xlink:to="loc_us-gaap_SeriesEPreferredStockMember_150df131-30d7-422c-bf0f-8c731197e6b5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_2b0fa830-0ec4-4445-9b19-94ff3df9d5e9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_c67c06ab-acb4-452a-85ec-3fff612f5079" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_2b0fa830-0ec4-4445-9b19-94ff3df9d5e9" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_2b0fa830-0ec4-4445-9b19-94ff3df9d5e9_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_2b0fa830-0ec4-4445-9b19-94ff3df9d5e9" xlink:to="loc_us-gaap_EquityComponentDomain_2b0fa830-0ec4-4445-9b19-94ff3df9d5e9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_6e733907-9fd2-4a55-a66a-00738ef1db19" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_2b0fa830-0ec4-4445-9b19-94ff3df9d5e9" xlink:to="loc_us-gaap_EquityComponentDomain_6e733907-9fd2-4a55-a66a-00738ef1db19" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockMember_0425be9a-22f0-4528-9be1-82fa5bf0e52d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_6e733907-9fd2-4a55-a66a-00738ef1db19" xlink:to="loc_us-gaap_PreferredStockMember_0425be9a-22f0-4528-9be1-82fa5bf0e52d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_NumberOfExecutiveOfficersServingAsDirectorsAndExecutiveOfficersOfAdviserAndAdministrator_67199ba4-5419-46b8-b82a-714cee65364b" xlink:href="land-20240630.xsd#land_NumberOfExecutiveOfficersServingAsDirectorsAndExecutiveOfficersOfAdviserAndAdministrator"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_4f6826c1-c69d-48f3-a099-c0943068d626" xlink:to="loc_land_NumberOfExecutiveOfficersServingAsDirectorsAndExecutiveOfficersOfAdviserAndAdministrator_67199ba4-5419-46b8-b82a-714cee65364b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ManagementAndServiceFeesBaseRate_3e2924e7-2a1a-4287-a271-6cfb6708c090" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ManagementAndServiceFeesBaseRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_4f6826c1-c69d-48f3-a099-c0943068d626" xlink:to="loc_us-gaap_ManagementAndServiceFeesBaseRate_3e2924e7-2a1a-4287-a271-6cfb6708c090" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ManagementAndServiceFeesIncentiveRate_1d898ae2-146e-41dc-b8d6-9a9d3f9f9441" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ManagementAndServiceFeesIncentiveRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_4f6826c1-c69d-48f3-a099-c0943068d626" xlink:to="loc_us-gaap_ManagementAndServiceFeesIncentiveRate_1d898ae2-146e-41dc-b8d6-9a9d3f9f9441" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_NoticePeriodForTerminationOfAgreementWithoutCause_6d3d3546-780c-4b91-a328-2acba8941b38" xlink:href="land-20240630.xsd#land_NoticePeriodForTerminationOfAgreementWithoutCause"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_4f6826c1-c69d-48f3-a099-c0943068d626" xlink:to="loc_land_NoticePeriodForTerminationOfAgreementWithoutCause_6d3d3546-780c-4b91-a328-2acba8941b38" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_TerminationAgreementPercentageOfIndependentDirectors_64882675-57a3-42e0-9042-dcf7b758a0f9" xlink:href="land-20240630.xsd#land_TerminationAgreementPercentageOfIndependentDirectors"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_4f6826c1-c69d-48f3-a099-c0943068d626" xlink:to="loc_land_TerminationAgreementPercentageOfIndependentDirectors_64882675-57a3-42e0-9042-dcf7b758a0f9" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_TerminationAgreementMultiplierForAverageAnnualBaseManagementFee_fe215370-9cfe-4b44-9f88-0ac040e94231" xlink:href="land-20240630.xsd#land_TerminationAgreementMultiplierForAverageAnnualBaseManagementFee"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_4f6826c1-c69d-48f3-a099-c0943068d626" xlink:to="loc_land_TerminationAgreementMultiplierForAverageAnnualBaseManagementFee_fe215370-9cfe-4b44-9f88-0ac040e94231" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_TerminationAgreementPeriodPriorToTermination_553535f4-f9a1-431c-850e-0d7e4c93a2e6" xlink:href="land-20240630.xsd#land_TerminationAgreementPeriodPriorToTermination"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_4f6826c1-c69d-48f3-a099-c0943068d626" xlink:to="loc_land_TerminationAgreementPeriodPriorToTermination_553535f4-f9a1-431c-850e-0d7e4c93a2e6" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionRate_71c860be-c193-4f35-a8fc-0403ffdd6a06" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyTransactionRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_4f6826c1-c69d-48f3-a099-c0943068d626" xlink:to="loc_us-gaap_RelatedPartyTransactionRate_71c860be-c193-4f35-a8fc-0403ffdd6a06" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionAmountsOfTransaction_0ead0021-31d6-4675-bb14-7c9b6820c5ef" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyTransactionAmountsOfTransaction"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_4f6826c1-c69d-48f3-a099-c0943068d626" xlink:to="loc_us-gaap_RelatedPartyTransactionAmountsOfTransaction_0ead0021-31d6-4675-bb14-7c9b6820c5ef" xlink:type="arc" order="8"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.gladstoneland.com/role/RelatedPartyTransactionsScheduleoftheTotalSellingCommissionsandDealerManagerFeesPaidDetails" xlink:type="simple" xlink:href="land-20240630.xsd#RelatedPartyTransactionsScheduleoftheTotalSellingCommissionsandDealerManagerFeesPaidDetails"/>
  <link:definitionLink xlink:role="http://www.gladstoneland.com/role/RelatedPartyTransactionsScheduleoftheTotalSellingCommissionsandDealerManagerFeesPaidDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionLineItems_b951aed4-c094-4037-a0bd-9923b39a9fea" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyTransactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_42e12c92-b1b1-4a04-b897-3b47c520a552" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_b951aed4-c094-4037-a0bd-9923b39a9fea" xlink:to="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_42e12c92-b1b1-4a04-b897-3b47c520a552" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_f404af90-1d06-4234-bae0-fdd7412f387e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_42e12c92-b1b1-4a04-b897-3b47c520a552" xlink:to="loc_us-gaap_StatementClassOfStockAxis_f404af90-1d06-4234-bae0-fdd7412f387e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_f404af90-1d06-4234-bae0-fdd7412f387e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_f404af90-1d06-4234-bae0-fdd7412f387e" xlink:to="loc_us-gaap_ClassOfStockDomain_f404af90-1d06-4234-bae0-fdd7412f387e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_04f01b6d-36ea-409f-bac8-0c5e194af952" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_f404af90-1d06-4234-bae0-fdd7412f387e" xlink:to="loc_us-gaap_ClassOfStockDomain_04f01b6d-36ea-409f-bac8-0c5e194af952" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesEPreferredStockMember_6c9241a7-d1a8-491d-9109-a62c60eb3b10" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesEPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_04f01b6d-36ea-409f-bac8-0c5e194af952" xlink:to="loc_us-gaap_SeriesEPreferredStockMember_6c9241a7-d1a8-491d-9109-a62c60eb3b10" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_3daa4686-d8b8-4b06-ab70-1bf88989c85f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_42e12c92-b1b1-4a04-b897-3b47c520a552" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_3daa4686-d8b8-4b06-ab70-1bf88989c85f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_3daa4686-d8b8-4b06-ab70-1bf88989c85f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_3daa4686-d8b8-4b06-ab70-1bf88989c85f" xlink:to="loc_us-gaap_EquityComponentDomain_3daa4686-d8b8-4b06-ab70-1bf88989c85f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_b56ab260-1819-4544-a287-a7925635d504" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_3daa4686-d8b8-4b06-ab70-1bf88989c85f" xlink:to="loc_us-gaap_EquityComponentDomain_b56ab260-1819-4544-a287-a7925635d504" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockMember_eb918acc-f065-4bf6-9264-268b954c0597" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_b56ab260-1819-4544-a287-a7925635d504" xlink:to="loc_us-gaap_PreferredStockMember_eb918acc-f065-4bf6-9264-268b954c0597" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionAxis_a65f430a-ef43-44e3-8310-ec81b00b9805" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyTransactionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_42e12c92-b1b1-4a04-b897-3b47c520a552" xlink:to="loc_us-gaap_RelatedPartyTransactionAxis_a65f430a-ef43-44e3-8310-ec81b00b9805" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionDomain_a65f430a-ef43-44e3-8310-ec81b00b9805_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RelatedPartyTransactionAxis_a65f430a-ef43-44e3-8310-ec81b00b9805" xlink:to="loc_us-gaap_RelatedPartyTransactionDomain_a65f430a-ef43-44e3-8310-ec81b00b9805_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionDomain_b525ac25-518a-4cdd-a31c-e23f953dcc73" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RelatedPartyTransactionAxis_a65f430a-ef43-44e3-8310-ec81b00b9805" xlink:to="loc_us-gaap_RelatedPartyTransactionDomain_b525ac25-518a-4cdd-a31c-e23f953dcc73" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_SellingCommissionsAndDealerManagementFeesMember_386fbe5a-7fff-4176-a772-f33e454209fe" xlink:href="land-20240630.xsd#land_SellingCommissionsAndDealerManagementFeesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_b525ac25-518a-4cdd-a31c-e23f953dcc73" xlink:to="loc_land_SellingCommissionsAndDealerManagementFeesMember_386fbe5a-7fff-4176-a772-f33e454209fe" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CounterpartyNameAxis_feba36c0-a194-4b54-bd9c-5d10ee336f97" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_CounterpartyNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_42e12c92-b1b1-4a04-b897-3b47c520a552" xlink:to="loc_srt_CounterpartyNameAxis_feba36c0-a194-4b54-bd9c-5d10ee336f97" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_feba36c0-a194-4b54-bd9c-5d10ee336f97_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CounterpartyNameAxis_feba36c0-a194-4b54-bd9c-5d10ee336f97" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_feba36c0-a194-4b54-bd9c-5d10ee336f97_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_6f537b5a-52da-416a-a3e1-259343a5d7e1" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CounterpartyNameAxis_feba36c0-a194-4b54-bd9c-5d10ee336f97" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_6f537b5a-52da-416a-a3e1-259343a5d7e1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_GladstoneSecuritiesMember_6dee0d65-8a9a-4158-94f9-6395189deb63" xlink:href="land-20240630.xsd#land_GladstoneSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_6f537b5a-52da-416a-a3e1-259343a5d7e1" xlink:to="loc_land_GladstoneSecuritiesMember_6dee0d65-8a9a-4158-94f9-6395189deb63" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionAmountsOfTransaction_d6db8bc0-bba7-43d7-8260-2a35b7767a33" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyTransactionAmountsOfTransaction"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_b951aed4-c094-4037-a0bd-9923b39a9fea" xlink:to="loc_us-gaap_RelatedPartyTransactionAmountsOfTransaction_d6db8bc0-bba7-43d7-8260-2a35b7767a33" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.gladstoneland.com/role/RelatedPartyTransactionsScheduleofManagementFeesIncentiveFeesandAssociatedCreditsandAdministrationFeesDetails" xlink:type="simple" xlink:href="land-20240630.xsd#RelatedPartyTransactionsScheduleofManagementFeesIncentiveFeesandAssociatedCreditsandAdministrationFeesDetails"/>
  <link:definitionLink xlink:role="http://www.gladstoneland.com/role/RelatedPartyTransactionsScheduleofManagementFeesIncentiveFeesandAssociatedCreditsandAdministrationFeesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionLineItems_654d006b-36af-4453-b389-2ec6d6236347" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyTransactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_40ef10cf-6555-499f-a783-ab0322167af6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_654d006b-36af-4453-b389-2ec6d6236347" xlink:to="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_40ef10cf-6555-499f-a783-ab0322167af6" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionAxis_47a58ccf-0a55-4b66-a6e0-a65f7fdc1a14" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyTransactionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_40ef10cf-6555-499f-a783-ab0322167af6" xlink:to="loc_us-gaap_RelatedPartyTransactionAxis_47a58ccf-0a55-4b66-a6e0-a65f7fdc1a14" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionDomain_47a58ccf-0a55-4b66-a6e0-a65f7fdc1a14_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RelatedPartyTransactionAxis_47a58ccf-0a55-4b66-a6e0-a65f7fdc1a14" xlink:to="loc_us-gaap_RelatedPartyTransactionDomain_47a58ccf-0a55-4b66-a6e0-a65f7fdc1a14_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionDomain_c28df806-f044-42cd-8a42-188a96380b1e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RelatedPartyTransactionAxis_47a58ccf-0a55-4b66-a6e0-a65f7fdc1a14" xlink:to="loc_us-gaap_RelatedPartyTransactionDomain_c28df806-f044-42cd-8a42-188a96380b1e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_BaseManagementFeeMember_0c9c30af-2965-4d8e-9161-3beba7ed4d8e" xlink:href="land-20240630.xsd#land_BaseManagementFeeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_c28df806-f044-42cd-8a42-188a96380b1e" xlink:to="loc_land_BaseManagementFeeMember_0c9c30af-2965-4d8e-9161-3beba7ed4d8e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_AdministrationFeeMember_80f444f8-de4e-4265-a083-8712785a8ad5" xlink:href="land-20240630.xsd#land_AdministrationFeeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_c28df806-f044-42cd-8a42-188a96380b1e" xlink:to="loc_land_AdministrationFeeMember_80f444f8-de4e-4265-a083-8712785a8ad5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_FinancingFeesAndSellingCommissionsAndDealerManagementFeesMember_93657bec-a4fb-4d59-a522-c82150c6ec12" xlink:href="land-20240630.xsd#land_FinancingFeesAndSellingCommissionsAndDealerManagementFeesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_c28df806-f044-42cd-8a42-188a96380b1e" xlink:to="loc_land_FinancingFeesAndSellingCommissionsAndDealerManagementFeesMember_93657bec-a4fb-4d59-a522-c82150c6ec12" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_SellingCommissionsAndDealerManagementFeesMember_dd74c7a1-0fe4-4df4-ae0c-9b50215a86d3" xlink:href="land-20240630.xsd#land_SellingCommissionsAndDealerManagementFeesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_land_FinancingFeesAndSellingCommissionsAndDealerManagementFeesMember_93657bec-a4fb-4d59-a522-c82150c6ec12" xlink:to="loc_land_SellingCommissionsAndDealerManagementFeesMember_dd74c7a1-0fe4-4df4-ae0c-9b50215a86d3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CounterpartyNameAxis_37ff5722-f0fa-4612-88ef-054eafeba955" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_CounterpartyNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_40ef10cf-6555-499f-a783-ab0322167af6" xlink:to="loc_srt_CounterpartyNameAxis_37ff5722-f0fa-4612-88ef-054eafeba955" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_37ff5722-f0fa-4612-88ef-054eafeba955_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CounterpartyNameAxis_37ff5722-f0fa-4612-88ef-054eafeba955" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_37ff5722-f0fa-4612-88ef-054eafeba955_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_2500434a-73ca-4ee3-9aee-ca582902db69" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CounterpartyNameAxis_37ff5722-f0fa-4612-88ef-054eafeba955" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_2500434a-73ca-4ee3-9aee-ca582902db69" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_AdviserMember_b2918df7-c32e-430a-a048-3da28455836f" xlink:href="land-20240630.xsd#land_AdviserMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_2500434a-73ca-4ee3-9aee-ca582902db69" xlink:to="loc_land_AdviserMember_b2918df7-c32e-430a-a048-3da28455836f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_GladstoneSecuritiesMember_25136498-b2f9-4780-99c1-9f235e6786e6" xlink:href="land-20240630.xsd#land_GladstoneSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_2500434a-73ca-4ee3-9aee-ca582902db69" xlink:to="loc_land_GladstoneSecuritiesMember_25136498-b2f9-4780-99c1-9f235e6786e6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionAmountsOfTransaction_23e24fb7-bfa3-4c79-9880-b7739b3950ec" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyTransactionAmountsOfTransaction"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_654d006b-36af-4453-b389-2ec6d6236347" xlink:to="loc_us-gaap_RelatedPartyTransactionAmountsOfTransaction_23e24fb7-bfa3-4c79-9880-b7739b3950ec" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.gladstoneland.com/role/RelatedPartyTransactionsDetailsofAmountsDuetoRelatedPartiesonOurAccompanyingCondensedConsolidatedBalanceSheetsDetails" xlink:type="simple" xlink:href="land-20240630.xsd#RelatedPartyTransactionsDetailsofAmountsDuetoRelatedPartiesonOurAccompanyingCondensedConsolidatedBalanceSheetsDetails"/>
  <link:definitionLink xlink:role="http://www.gladstoneland.com/role/RelatedPartyTransactionsDetailsofAmountsDuetoRelatedPartiesonOurAccompanyingCondensedConsolidatedBalanceSheetsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionLineItems_bc248374-303c-4762-921f-106d1bd211bc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyTransactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_de3a171b-c343-4fb2-8b31-d98dbe68d535" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_bc248374-303c-4762-921f-106d1bd211bc" xlink:to="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_de3a171b-c343-4fb2-8b31-d98dbe68d535" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionAxis_d9efad4c-ad1d-42b7-9a71-cf6c43584713" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyTransactionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_de3a171b-c343-4fb2-8b31-d98dbe68d535" xlink:to="loc_us-gaap_RelatedPartyTransactionAxis_d9efad4c-ad1d-42b7-9a71-cf6c43584713" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionDomain_d9efad4c-ad1d-42b7-9a71-cf6c43584713_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RelatedPartyTransactionAxis_d9efad4c-ad1d-42b7-9a71-cf6c43584713" xlink:to="loc_us-gaap_RelatedPartyTransactionDomain_d9efad4c-ad1d-42b7-9a71-cf6c43584713_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionDomain_03ad3531-1913-469b-9b63-3bc9ba7ea6dc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RelatedPartyTransactionAxis_d9efad4c-ad1d-42b7-9a71-cf6c43584713" xlink:to="loc_us-gaap_RelatedPartyTransactionDomain_03ad3531-1913-469b-9b63-3bc9ba7ea6dc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_BaseManagementFeeMember_5b958b20-be1c-413b-bd42-e759c59b4d4e" xlink:href="land-20240630.xsd#land_BaseManagementFeeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_03ad3531-1913-469b-9b63-3bc9ba7ea6dc" xlink:to="loc_land_BaseManagementFeeMember_5b958b20-be1c-413b-bd42-e759c59b4d4e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_IncentiveFeeMember_0d1fb1dd-36ef-42f6-808d-471319dd3551" xlink:href="land-20240630.xsd#land_IncentiveFeeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_03ad3531-1913-469b-9b63-3bc9ba7ea6dc" xlink:to="loc_land_IncentiveFeeMember_0d1fb1dd-36ef-42f6-808d-471319dd3551" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_MiscellaneousGeneralAndAdministrativeExpensesMember_e89f00df-e95b-4ed9-b5e2-e0cb2cd5513a" xlink:href="land-20240630.xsd#land_MiscellaneousGeneralAndAdministrativeExpensesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_03ad3531-1913-469b-9b63-3bc9ba7ea6dc" xlink:to="loc_land_MiscellaneousGeneralAndAdministrativeExpensesMember_e89f00df-e95b-4ed9-b5e2-e0cb2cd5513a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_AdministrationFeeMember_03cb07ff-8bf2-44b1-ad7d-2ad2b38016af" xlink:href="land-20240630.xsd#land_AdministrationFeeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_03ad3531-1913-469b-9b63-3bc9ba7ea6dc" xlink:to="loc_land_AdministrationFeeMember_03cb07ff-8bf2-44b1-ad7d-2ad2b38016af" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_CumulativeAccruedButUnpaidPortionOfPriorAdministrativeFeesMember_396ff4cf-b2c8-459c-a2b5-ddc0a9616875" xlink:href="land-20240630.xsd#land_CumulativeAccruedButUnpaidPortionOfPriorAdministrativeFeesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_03ad3531-1913-469b-9b63-3bc9ba7ea6dc" xlink:to="loc_land_CumulativeAccruedButUnpaidPortionOfPriorAdministrativeFeesMember_396ff4cf-b2c8-459c-a2b5-ddc0a9616875" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_82df73dc-fcc1-4116-af49-41a2bef1ade3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyTransactionsByRelatedPartyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_de3a171b-c343-4fb2-8b31-d98dbe68d535" xlink:to="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_82df73dc-fcc1-4116-af49-41a2bef1ade3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_82df73dc-fcc1-4116-af49-41a2bef1ade3_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_82df73dc-fcc1-4116-af49-41a2bef1ade3" xlink:to="loc_us-gaap_RelatedPartyDomain_82df73dc-fcc1-4116-af49-41a2bef1ade3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_5a79d26b-f86f-4927-839f-cb1c5174752c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_82df73dc-fcc1-4116-af49-41a2bef1ade3" xlink:to="loc_us-gaap_RelatedPartyDomain_5a79d26b-f86f-4927-839f-cb1c5174752c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyMember_34b800a4-784d-4229-9687-8ac3928ec8b9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RelatedPartyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyDomain_5a79d26b-f86f-4927-839f-cb1c5174752c" xlink:to="loc_us-gaap_RelatedPartyMember_34b800a4-784d-4229-9687-8ac3928ec8b9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CounterpartyNameAxis_a8acf08c-fdda-463f-9838-6c7691f2f0a4" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_CounterpartyNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_de3a171b-c343-4fb2-8b31-d98dbe68d535" xlink:to="loc_srt_CounterpartyNameAxis_a8acf08c-fdda-463f-9838-6c7691f2f0a4" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_a8acf08c-fdda-463f-9838-6c7691f2f0a4_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CounterpartyNameAxis_a8acf08c-fdda-463f-9838-6c7691f2f0a4" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_a8acf08c-fdda-463f-9838-6c7691f2f0a4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_04d2dbb6-a012-4035-a781-232c2ca80189" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CounterpartyNameAxis_a8acf08c-fdda-463f-9838-6c7691f2f0a4" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_04d2dbb6-a012-4035-a781-232c2ca80189" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_AdviserMember_edfe4663-ebeb-4264-880a-7f6c7ff83912" xlink:href="land-20240630.xsd#land_AdviserMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_04d2dbb6-a012-4035-a781-232c2ca80189" xlink:to="loc_land_AdviserMember_edfe4663-ebeb-4264-880a-7f6c7ff83912" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_AdministratorMember_5fa205e4-ca84-4887-a014-8347ef4ef68a" xlink:href="land-20240630.xsd#land_AdministratorMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_04d2dbb6-a012-4035-a781-232c2ca80189" xlink:to="loc_land_AdministratorMember_5fa205e4-ca84-4887-a014-8347ef4ef68a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilities_ce0a86e3-642d-42fd-99cd-8fcfe6a87e1a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_bc248374-303c-4762-921f-106d1bd211bc" xlink:to="loc_us-gaap_OtherLiabilities_ce0a86e3-642d-42fd-99cd-8fcfe6a87e1a" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.gladstoneland.com/role/EquityAdditionalInformationDetails" xlink:type="simple" xlink:href="land-20240630.xsd#EquityAdditionalInformationDetails"/>
  <link:definitionLink xlink:role="http://www.gladstoneland.com/role/EquityAdditionalInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsidiarySaleOfStockLineItems_1321d454-652e-460a-9379-7cdf9b3c0fd0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsidiarySaleOfStockLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsidiaryOrEquityMethodInvesteeSaleOfStockBySubsidiaryOrEquityInvesteeTable_8cb94285-f097-4a0d-9922-035327097980" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsidiaryOrEquityMethodInvesteeSaleOfStockBySubsidiaryOrEquityInvesteeTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_1321d454-652e-460a-9379-7cdf9b3c0fd0" xlink:to="loc_us-gaap_SubsidiaryOrEquityMethodInvesteeSaleOfStockBySubsidiaryOrEquityInvesteeTable_8cb94285-f097-4a0d-9922-035327097980" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsidiarySaleOfStockAxis_da220f41-5251-41bb-81ae-804d82dfda0e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsidiarySaleOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsidiaryOrEquityMethodInvesteeSaleOfStockBySubsidiaryOrEquityInvesteeTable_8cb94285-f097-4a0d-9922-035327097980" xlink:to="loc_us-gaap_SubsidiarySaleOfStockAxis_da220f41-5251-41bb-81ae-804d82dfda0e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_da220f41-5251-41bb-81ae-804d82dfda0e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_da220f41-5251-41bb-81ae-804d82dfda0e" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_da220f41-5251-41bb-81ae-804d82dfda0e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_200938da-fc3a-448b-892d-49b334055fcd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_da220f41-5251-41bb-81ae-804d82dfda0e" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_200938da-fc3a-448b-892d-49b334055fcd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_UniversalRegistrationStatementThreeMember_d1a9d0ca-b854-443d-878d-53c7244b5d25" xlink:href="land-20240630.xsd#land_UniversalRegistrationStatementThreeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SaleOfStockNameOfTransactionDomain_200938da-fc3a-448b-892d-49b334055fcd" xlink:to="loc_land_UniversalRegistrationStatementThreeMember_d1a9d0ca-b854-443d-878d-53c7244b5d25" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_AtMarketProgramMember_ac3ecd90-5355-45cb-939f-63f3fb7e190c" xlink:href="land-20240630.xsd#land_AtMarketProgramMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SaleOfStockNameOfTransactionDomain_200938da-fc3a-448b-892d-49b334055fcd" xlink:to="loc_land_AtMarketProgramMember_ac3ecd90-5355-45cb-939f-63f3fb7e190c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_89ee4e05-e914-448e-a266-84c037af471c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsidiaryOrEquityMethodInvesteeSaleOfStockBySubsidiaryOrEquityInvesteeTable_8cb94285-f097-4a0d-9922-035327097980" xlink:to="loc_us-gaap_StatementClassOfStockAxis_89ee4e05-e914-448e-a266-84c037af471c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_89ee4e05-e914-448e-a266-84c037af471c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_89ee4e05-e914-448e-a266-84c037af471c" xlink:to="loc_us-gaap_ClassOfStockDomain_89ee4e05-e914-448e-a266-84c037af471c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_718d5afe-df8b-490d-9705-f2004d4eb9f7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_89ee4e05-e914-448e-a266-84c037af471c" xlink:to="loc_us-gaap_ClassOfStockDomain_718d5afe-df8b-490d-9705-f2004d4eb9f7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesCPreferredStockMember_e3729a92-aa71-4d5f-9a27-75d4add77346" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesCPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_718d5afe-df8b-490d-9705-f2004d4eb9f7" xlink:to="loc_us-gaap_SeriesCPreferredStockMember_e3729a92-aa71-4d5f-9a27-75d4add77346" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesDPreferredStockMember_005b38fa-e729-4719-abd4-e3a5663c7851" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesDPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_718d5afe-df8b-490d-9705-f2004d4eb9f7" xlink:to="loc_us-gaap_SeriesDPreferredStockMember_005b38fa-e729-4719-abd4-e3a5663c7851" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesEPreferredStockMember_ca84d2d4-16fd-4a98-ba9a-c3d8208fe931" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesEPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_718d5afe-df8b-490d-9705-f2004d4eb9f7" xlink:to="loc_us-gaap_SeriesEPreferredStockMember_ca84d2d4-16fd-4a98-ba9a-c3d8208fe931" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_2cad14e2-f574-470c-9486-9f6e7a549724" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_718d5afe-df8b-490d-9705-f2004d4eb9f7" xlink:to="loc_us-gaap_CommonStockMember_2cad14e2-f574-470c-9486-9f6e7a549724" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesBPreferredStockMember_de405b04-dd3d-4e83-a10d-0eebfeee7bdf" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesBPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_718d5afe-df8b-490d-9705-f2004d4eb9f7" xlink:to="loc_us-gaap_SeriesBPreferredStockMember_de405b04-dd3d-4e83-a10d-0eebfeee7bdf" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_f2fe7fb0-48c3-43e6-85d9-bd07833540b1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsidiaryOrEquityMethodInvesteeSaleOfStockBySubsidiaryOrEquityInvesteeTable_8cb94285-f097-4a0d-9922-035327097980" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_f2fe7fb0-48c3-43e6-85d9-bd07833540b1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_f2fe7fb0-48c3-43e6-85d9-bd07833540b1_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_f2fe7fb0-48c3-43e6-85d9-bd07833540b1" xlink:to="loc_us-gaap_EquityComponentDomain_f2fe7fb0-48c3-43e6-85d9-bd07833540b1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_8cf29582-b461-4cf5-90f2-1d31eb9e7a25" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_f2fe7fb0-48c3-43e6-85d9-bd07833540b1" xlink:to="loc_us-gaap_EquityComponentDomain_8cf29582-b461-4cf5-90f2-1d31eb9e7a25" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockMember_1069229c-d380-4aa8-9f3e-ea4ccc69642c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_8cf29582-b461-4cf5-90f2-1d31eb9e7a25" xlink:to="loc_us-gaap_PreferredStockMember_1069229c-d380-4aa8-9f3e-ea4ccc69642c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_919f3a50-cff0-4f5d-ad13-3bdb261a99a3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_8cf29582-b461-4cf5-90f2-1d31eb9e7a25" xlink:to="loc_us-gaap_CommonStockMember_919f3a50-cff0-4f5d-ad13-3bdb261a99a3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_OwnershipAxis_929110ff-65fd-4c69-8772-68dfcb9af713" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_OwnershipAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsidiaryOrEquityMethodInvesteeSaleOfStockBySubsidiaryOrEquityInvesteeTable_8cb94285-f097-4a0d-9922-035327097980" xlink:to="loc_srt_OwnershipAxis_929110ff-65fd-4c69-8772-68dfcb9af713" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_OwnershipDomain_929110ff-65fd-4c69-8772-68dfcb9af713_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_OwnershipDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_OwnershipAxis_929110ff-65fd-4c69-8772-68dfcb9af713" xlink:to="loc_srt_OwnershipDomain_929110ff-65fd-4c69-8772-68dfcb9af713_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_OwnershipDomain_37079902-850c-497a-af18-cfedcb5501c6" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_OwnershipDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_OwnershipAxis_929110ff-65fd-4c69-8772-68dfcb9af713" xlink:to="loc_srt_OwnershipDomain_37079902-850c-497a-af18-cfedcb5501c6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_OPUnitHolderMember_f5d4fd30-cfa4-430b-9fc7-bf894b9b7ac3" xlink:href="land-20240630.xsd#land_OPUnitHolderMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_OwnershipDomain_37079902-850c-497a-af18-cfedcb5501c6" xlink:to="loc_land_OPUnitHolderMember_f5d4fd30-cfa4-430b-9fc7-bf894b9b7ac3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_ValueOfSecuritiesAllowedForIssuance_1dc2043b-19f3-4bd0-bfcd-67efd1b74a52" xlink:href="land-20240630.xsd#land_ValueOfSecuritiesAllowedForIssuance"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_1321d454-652e-460a-9379-7cdf9b3c0fd0" xlink:to="loc_land_ValueOfSecuritiesAllowedForIssuance_1dc2043b-19f3-4bd0-bfcd-67efd1b74a52" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_MaximumNumberOfSecuritiesCanBeSoldUnderShelfRegistrationStatement_f05309d7-f773-4ab5-a845-1507215d31c9" xlink:href="land-20240630.xsd#land_MaximumNumberOfSecuritiesCanBeSoldUnderShelfRegistrationStatement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_1321d454-652e-460a-9379-7cdf9b3c0fd0" xlink:to="loc_land_MaximumNumberOfSecuritiesCanBeSoldUnderShelfRegistrationStatement_f05309d7-f773-4ab5-a845-1507215d31c9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesIssued_c9a8a6dd-29fe-4cd0-b817-ebdf3441df58" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_1321d454-652e-460a-9379-7cdf9b3c0fd0" xlink:to="loc_us-gaap_PreferredStockSharesIssued_c9a8a6dd-29fe-4cd0-b817-ebdf3441df58" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOrSaleOfEquity_bd5116e2-5be3-404a-852e-738b0174031c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromIssuanceOrSaleOfEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_1321d454-652e-460a-9379-7cdf9b3c0fd0" xlink:to="loc_us-gaap_ProceedsFromIssuanceOrSaleOfEquity_bd5116e2-5be3-404a-852e-738b0174031c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesIssued_ad698d15-c3ff-403b-9ffb-cad1e04b862f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_1321d454-652e-460a-9379-7cdf9b3c0fd0" xlink:to="loc_us-gaap_CommonStockSharesIssued_ad698d15-c3ff-403b-9ffb-cad1e04b862f" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockDividendRatePercentage_ecb56823-0849-4d7d-a36e-e626b76c9def" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockDividendRatePercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_1321d454-652e-460a-9379-7cdf9b3c0fd0" xlink:to="loc_us-gaap_PreferredStockDividendRatePercentage_ecb56823-0849-4d7d-a36e-e626b76c9def" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockParOrStatedValuePerShare_27c9d10e-8013-4a05-a060-cb59358bb7c0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockParOrStatedValuePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_1321d454-652e-460a-9379-7cdf9b3c0fd0" xlink:to="loc_us-gaap_PreferredStockParOrStatedValuePerShare_27c9d10e-8013-4a05-a060-cb59358bb7c0" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesAuthorized_d440ee47-793c-4bc0-b1de-f25504015257" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_1321d454-652e-460a-9379-7cdf9b3c0fd0" xlink:to="loc_us-gaap_PreferredStockSharesAuthorized_d440ee47-793c-4bc0-b1de-f25504015257" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockPricePerShare_3c985eab-68ed-46fd-a224-956a201639b3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SaleOfStockPricePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_1321d454-652e-460a-9379-7cdf9b3c0fd0" xlink:to="loc_us-gaap_SaleOfStockPricePerShare_3c985eab-68ed-46fd-a224-956a201639b3" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_PreferredStockSharesAuthorizedDividendReinvestmentPlan_7fbed70a-116f-4c6c-b7ed-eb69abd25b64" xlink:href="land-20240630.xsd#land_PreferredStockSharesAuthorizedDividendReinvestmentPlan"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_1321d454-652e-460a-9379-7cdf9b3c0fd0" xlink:to="loc_land_PreferredStockSharesAuthorizedDividendReinvestmentPlan_7fbed70a-116f-4c6c-b7ed-eb69abd25b64" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_SaleOfStockPricePerShareDividendReinvestmentPlan_8344de4f-c9d2-4bdc-9349-f752bebe5516" xlink:href="land-20240630.xsd#land_SaleOfStockPricePerShareDividendReinvestmentPlan"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_1321d454-652e-460a-9379-7cdf9b3c0fd0" xlink:to="loc_land_SaleOfStockPricePerShareDividendReinvestmentPlan_8344de4f-c9d2-4bdc-9349-f752bebe5516" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_SaleOfStockConsiderationReceivedOnTransactionGross_f71e8b23-6175-4d94-86f3-19f82a842bb6" xlink:href="land-20240630.xsd#land_SaleOfStockConsiderationReceivedOnTransactionGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_1321d454-652e-460a-9379-7cdf9b3c0fd0" xlink:to="loc_land_SaleOfStockConsiderationReceivedOnTransactionGross_f71e8b23-6175-4d94-86f3-19f82a842bb6" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_SaleOfStockConsiderationReceivedNet_04bed5c0-2c15-481c-a9ed-5a2285f82be8" xlink:href="land-20240630.xsd#land_SaleOfStockConsiderationReceivedNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_1321d454-652e-460a-9379-7cdf9b3c0fd0" xlink:to="loc_land_SaleOfStockConsiderationReceivedNet_04bed5c0-2c15-481c-a9ed-5a2285f82be8" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_PreferredStockSharesIssuedDividendReinvestmentPlan_3cfd2940-22de-49ba-88f6-beeddf385eaa" xlink:href="land-20240630.xsd#land_PreferredStockSharesIssuedDividendReinvestmentPlan"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_1321d454-652e-460a-9379-7cdf9b3c0fd0" xlink:to="loc_land_PreferredStockSharesIssuedDividendReinvestmentPlan_3cfd2940-22de-49ba-88f6-beeddf385eaa" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockRedeemedOrCalledDuringPeriodShares_70f12e9f-0995-4b9c-a236-28a43b656342" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockRedeemedOrCalledDuringPeriodShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_1321d454-652e-460a-9379-7cdf9b3c0fd0" xlink:to="loc_us-gaap_StockRedeemedOrCalledDuringPeriodShares_70f12e9f-0995-4b9c-a236-28a43b656342" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockRedeemedOrCalledDuringPeriodValue_8599977b-d7e6-486a-9caf-63fdc7b3f4d0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockRedeemedOrCalledDuringPeriodValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_1321d454-652e-460a-9379-7cdf9b3c0fd0" xlink:to="loc_us-gaap_StockRedeemedOrCalledDuringPeriodValue_8599977b-d7e6-486a-9caf-63fdc7b3f4d0" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_MaximumAmountOfSecuritiesCanBeSoldUnderShelfRegistrationStatement_03f7cfaa-754b-44f5-9ec8-0277a05c643c" xlink:href="land-20240630.xsd#land_MaximumAmountOfSecuritiesCanBeSoldUnderShelfRegistrationStatement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_1321d454-652e-460a-9379-7cdf9b3c0fd0" xlink:to="loc_land_MaximumAmountOfSecuritiesCanBeSoldUnderShelfRegistrationStatement_03f7cfaa-754b-44f5-9ec8-0277a05c643c" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_CommonStockValueAuthorizedUnderEquityOfferingProgram_547b61e5-b953-47ba-a031-b0857bfdf5dc" xlink:href="land-20240630.xsd#land_CommonStockValueAuthorizedUnderEquityOfferingProgram"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_1321d454-652e-460a-9379-7cdf9b3c0fd0" xlink:to="loc_land_CommonStockValueAuthorizedUnderEquityOfferingProgram_547b61e5-b953-47ba-a031-b0857bfdf5dc" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StockRepurchaseProgramAuthorizedAmount1_c54e437c-a91e-4150-a5d0-bd277e5181c4" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_StockRepurchaseProgramAuthorizedAmount1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_1321d454-652e-460a-9379-7cdf9b3c0fd0" xlink:to="loc_srt_StockRepurchaseProgramAuthorizedAmount1_c54e437c-a91e-4150-a5d0-bd277e5181c4" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MinorityInterestOwnershipPercentageByParent_66928559-b9c4-4949-ab3a-8172b1d25c3e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_MinorityInterestOwnershipPercentageByParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_1321d454-652e-460a-9379-7cdf9b3c0fd0" xlink:to="loc_us-gaap_MinorityInterestOwnershipPercentageByParent_66928559-b9c4-4949-ab3a-8172b1d25c3e" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_NoncontrollingInterestMinimumOwnershipPeriodRequiredToConvertUnitsHeld_5014477a-04f0-4427-86bb-e285d5bf34f6" xlink:href="land-20240630.xsd#land_NoncontrollingInterestMinimumOwnershipPeriodRequiredToConvertUnitsHeld"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_1321d454-652e-460a-9379-7cdf9b3c0fd0" xlink:to="loc_land_NoncontrollingInterestMinimumOwnershipPeriodRequiredToConvertUnitsHeld_5014477a-04f0-4427-86bb-e285d5bf34f6" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_OperatingUnitsConversionRatio_191e6c7d-e5ae-42e4-a67a-a872eb8968cd" xlink:href="land-20240630.xsd#land_OperatingUnitsConversionRatio"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_1321d454-652e-460a-9379-7cdf9b3c0fd0" xlink:to="loc_land_OperatingUnitsConversionRatio_191e6c7d-e5ae-42e4-a67a-a872eb8968cd" xlink:type="arc" order="21"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.gladstoneland.com/role/EquityEquityIssuancesDetails" xlink:type="simple" xlink:href="land-20240630.xsd#EquityEquityIssuancesDetails"/>
  <link:definitionLink xlink:role="http://www.gladstoneland.com/role/EquityEquityIssuancesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockLineItems_e8dd1f07-79e6-4843-86f9-7f0694cd8dd0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfStockByClassTable_130ab7e6-a163-448f-917f-c4e6fca47217" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfStockByClassTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ClassOfStockLineItems_e8dd1f07-79e6-4843-86f9-7f0694cd8dd0" xlink:to="loc_us-gaap_ScheduleOfStockByClassTable_130ab7e6-a163-448f-917f-c4e6fca47217" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_67a624d6-6edc-4c1f-9794-9d6e4ed5fce6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_130ab7e6-a163-448f-917f-c4e6fca47217" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_67a624d6-6edc-4c1f-9794-9d6e4ed5fce6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_67a624d6-6edc-4c1f-9794-9d6e4ed5fce6_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_67a624d6-6edc-4c1f-9794-9d6e4ed5fce6" xlink:to="loc_us-gaap_EquityComponentDomain_67a624d6-6edc-4c1f-9794-9d6e4ed5fce6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_a35af263-088a-4716-ae66-fbd114de8e03" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_67a624d6-6edc-4c1f-9794-9d6e4ed5fce6" xlink:to="loc_us-gaap_EquityComponentDomain_a35af263-088a-4716-ae66-fbd114de8e03" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockMember_95a4a048-4a76-4a8b-991d-e26e3c1dce02" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_a35af263-088a-4716-ae66-fbd114de8e03" xlink:to="loc_us-gaap_PreferredStockMember_95a4a048-4a76-4a8b-991d-e26e3c1dce02" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_b16c8097-e4a2-43aa-b574-71d99b67ea5b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_130ab7e6-a163-448f-917f-c4e6fca47217" xlink:to="loc_us-gaap_StatementClassOfStockAxis_b16c8097-e4a2-43aa-b574-71d99b67ea5b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_b16c8097-e4a2-43aa-b574-71d99b67ea5b_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_b16c8097-e4a2-43aa-b574-71d99b67ea5b" xlink:to="loc_us-gaap_ClassOfStockDomain_b16c8097-e4a2-43aa-b574-71d99b67ea5b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_2d23b026-35d0-4cc3-94d6-7cc5a520fb24" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_b16c8097-e4a2-43aa-b574-71d99b67ea5b" xlink:to="loc_us-gaap_ClassOfStockDomain_2d23b026-35d0-4cc3-94d6-7cc5a520fb24" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesEPreferredStockMember_fe6278f5-7a00-4add-8b17-19f917b97327" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesEPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_2d23b026-35d0-4cc3-94d6-7cc5a520fb24" xlink:to="loc_us-gaap_SeriesEPreferredStockMember_fe6278f5-7a00-4add-8b17-19f917b97327" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_64e7987e-0718-493f-af7e-8e262903c53b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_2d23b026-35d0-4cc3-94d6-7cc5a520fb24" xlink:to="loc_us-gaap_CommonStockMember_64e7987e-0718-493f-af7e-8e262903c53b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsidiarySaleOfStockAxis_612fa1f7-816e-4869-9d55-93e18e39aba3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsidiarySaleOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_130ab7e6-a163-448f-917f-c4e6fca47217" xlink:to="loc_us-gaap_SubsidiarySaleOfStockAxis_612fa1f7-816e-4869-9d55-93e18e39aba3" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_612fa1f7-816e-4869-9d55-93e18e39aba3_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_612fa1f7-816e-4869-9d55-93e18e39aba3" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_612fa1f7-816e-4869-9d55-93e18e39aba3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_93042e9e-b3af-487e-af6c-e98b10231f07" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_612fa1f7-816e-4869-9d55-93e18e39aba3" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_93042e9e-b3af-487e-af6c-e98b10231f07" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_AtMarketProgramMember_3d736067-073c-42a1-bc80-b231435f6212" xlink:href="land-20240630.xsd#land_AtMarketProgramMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SaleOfStockNameOfTransactionDomain_93042e9e-b3af-487e-af6c-e98b10231f07" xlink:to="loc_land_AtMarketProgramMember_3d736067-073c-42a1-bc80-b231435f6212" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction_c34a5fe3-0b1e-430e-aca7-5ceea69192ba" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_e8dd1f07-79e6-4843-86f9-7f0694cd8dd0" xlink:to="loc_us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction_c34a5fe3-0b1e-430e-aca7-5ceea69192ba" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_SaleOfStockWeightedAveragePricePerShare_7bc7fd52-fa6f-48fb-92dc-0107b514b94f" xlink:href="land-20240630.xsd#land_SaleOfStockWeightedAveragePricePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_e8dd1f07-79e6-4843-86f9-7f0694cd8dd0" xlink:to="loc_land_SaleOfStockWeightedAveragePricePerShare_7bc7fd52-fa6f-48fb-92dc-0107b514b94f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOrSaleOfEquity_0545597a-4629-4a0b-80cc-f7fe899284b1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromIssuanceOrSaleOfEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_e8dd1f07-79e6-4843-86f9-7f0694cd8dd0" xlink:to="loc_us-gaap_ProceedsFromIssuanceOrSaleOfEquity_0545597a-4629-4a0b-80cc-f7fe899284b1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockConsiderationReceivedOnTransaction_3c4a0f1b-b0e8-4c14-818f-fbec497a0602" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SaleOfStockConsiderationReceivedOnTransaction"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_e8dd1f07-79e6-4843-86f9-7f0694cd8dd0" xlink:to="loc_us-gaap_SaleOfStockConsiderationReceivedOnTransaction_3c4a0f1b-b0e8-4c14-818f-fbec497a0602" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.gladstoneland.com/role/EquityEquityRepurchaseActivityDetails" xlink:type="simple" xlink:href="land-20240630.xsd#EquityEquityRepurchaseActivityDetails"/>
  <link:definitionLink xlink:role="http://www.gladstoneland.com/role/EquityEquityRepurchaseActivityDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockLineItems_a641b50e-9c30-415f-b00c-4fac6c878ce3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfStockByClassTable_443aec8e-6d44-4f8f-b0e3-9e081d9c44f8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfStockByClassTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ClassOfStockLineItems_a641b50e-9c30-415f-b00c-4fac6c878ce3" xlink:to="loc_us-gaap_ScheduleOfStockByClassTable_443aec8e-6d44-4f8f-b0e3-9e081d9c44f8" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_77f97611-0691-43fb-b2a2-5a173f7867a1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_443aec8e-6d44-4f8f-b0e3-9e081d9c44f8" xlink:to="loc_us-gaap_StatementClassOfStockAxis_77f97611-0691-43fb-b2a2-5a173f7867a1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_77f97611-0691-43fb-b2a2-5a173f7867a1_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_77f97611-0691-43fb-b2a2-5a173f7867a1" xlink:to="loc_us-gaap_ClassOfStockDomain_77f97611-0691-43fb-b2a2-5a173f7867a1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_afc3b913-c21d-4fdc-b2e4-50e93136d5e8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_77f97611-0691-43fb-b2a2-5a173f7867a1" xlink:to="loc_us-gaap_ClassOfStockDomain_afc3b913-c21d-4fdc-b2e4-50e93136d5e8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesBPreferredStockMember_0d4ab389-e67c-43fa-ae68-f6a3a3c3f2ed" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesBPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_afc3b913-c21d-4fdc-b2e4-50e93136d5e8" xlink:to="loc_us-gaap_SeriesBPreferredStockMember_0d4ab389-e67c-43fa-ae68-f6a3a3c3f2ed" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesCPreferredStockMember_069391d9-ea24-4478-8960-8faa22cd8c5f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesCPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_afc3b913-c21d-4fdc-b2e4-50e93136d5e8" xlink:to="loc_us-gaap_SeriesCPreferredStockMember_069391d9-ea24-4478-8960-8faa22cd8c5f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_4cffa323-bdaa-4ca7-99fa-7f19e8a717f9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_443aec8e-6d44-4f8f-b0e3-9e081d9c44f8" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_4cffa323-bdaa-4ca7-99fa-7f19e8a717f9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_4cffa323-bdaa-4ca7-99fa-7f19e8a717f9_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_4cffa323-bdaa-4ca7-99fa-7f19e8a717f9" xlink:to="loc_us-gaap_EquityComponentDomain_4cffa323-bdaa-4ca7-99fa-7f19e8a717f9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_978b052e-72ed-4d08-8c0f-b6a47efa1b67" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_4cffa323-bdaa-4ca7-99fa-7f19e8a717f9" xlink:to="loc_us-gaap_EquityComponentDomain_978b052e-72ed-4d08-8c0f-b6a47efa1b67" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockMember_a7d93fbf-8d22-41bc-803d-59848d9264d2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_978b052e-72ed-4d08-8c0f-b6a47efa1b67" xlink:to="loc_us-gaap_PreferredStockMember_a7d93fbf-8d22-41bc-803d-59848d9264d2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockRepurchasedDuringPeriodShares_c00a63a4-3eb2-44c1-ad4d-d969a954676e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockRepurchasedDuringPeriodShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_a641b50e-9c30-415f-b00c-4fac6c878ce3" xlink:to="loc_us-gaap_StockRepurchasedDuringPeriodShares_c00a63a4-3eb2-44c1-ad4d-d969a954676e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockRepurchasedDuringPeriodValue_152137fd-86e9-461a-b9a8-8b7c68b51c09" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockRepurchasedDuringPeriodValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_a641b50e-9c30-415f-b00c-4fac6c878ce3" xlink:to="loc_us-gaap_StockRepurchasedDuringPeriodValue_152137fd-86e9-461a-b9a8-8b7c68b51c09" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockAcquiredAverageCostPerShare_7c840cc6-a406-4938-86cf-acc9310f3a99" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_TreasuryStockAcquiredAverageCostPerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_a641b50e-9c30-415f-b00c-4fac6c878ce3" xlink:to="loc_us-gaap_TreasuryStockAcquiredAverageCostPerShare_7c840cc6-a406-4938-86cf-acc9310f3a99" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherPreferredStockDividendsAndAdjustments_4a657de0-4026-468b-8d38-e9dcf2a228b7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherPreferredStockDividendsAndAdjustments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_a641b50e-9c30-415f-b00c-4fac6c878ce3" xlink:to="loc_us-gaap_OtherPreferredStockDividendsAndAdjustments_4a657de0-4026-468b-8d38-e9dcf2a228b7" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.gladstoneland.com/role/EquityMonthlyDistributionsDeclaredandPaidbyCompanysBoardofDirectorsDetails" xlink:type="simple" xlink:href="land-20240630.xsd#EquityMonthlyDistributionsDeclaredandPaidbyCompanysBoardofDirectorsDetails"/>
  <link:definitionLink xlink:role="http://www.gladstoneland.com/role/EquityMonthlyDistributionsDeclaredandPaidbyCompanysBoardofDirectorsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockLineItems_b392d7a7-d2a5-4df4-8879-fd77aba5ec49" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfStockByClassTable_786dbb18-0f4e-4370-b660-415c1fae8692" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfStockByClassTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ClassOfStockLineItems_b392d7a7-d2a5-4df4-8879-fd77aba5ec49" xlink:to="loc_us-gaap_ScheduleOfStockByClassTable_786dbb18-0f4e-4370-b660-415c1fae8692" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_b806769b-aadf-4ba0-a7de-15ebc208a62e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_786dbb18-0f4e-4370-b660-415c1fae8692" xlink:to="loc_us-gaap_StatementClassOfStockAxis_b806769b-aadf-4ba0-a7de-15ebc208a62e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_b806769b-aadf-4ba0-a7de-15ebc208a62e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_b806769b-aadf-4ba0-a7de-15ebc208a62e" xlink:to="loc_us-gaap_ClassOfStockDomain_b806769b-aadf-4ba0-a7de-15ebc208a62e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_f54588e4-a230-4e09-a0a1-c6c252c898d2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_b806769b-aadf-4ba0-a7de-15ebc208a62e" xlink:to="loc_us-gaap_ClassOfStockDomain_f54588e4-a230-4e09-a0a1-c6c252c898d2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesBPreferredStockMember_42628f9c-a2eb-4a96-84f3-7009c9e72b32" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesBPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_f54588e4-a230-4e09-a0a1-c6c252c898d2" xlink:to="loc_us-gaap_SeriesBPreferredStockMember_42628f9c-a2eb-4a96-84f3-7009c9e72b32" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesCPreferredStockMember_09ea7c48-79c5-43f1-8fdf-6c378a1fc49d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesCPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_f54588e4-a230-4e09-a0a1-c6c252c898d2" xlink:to="loc_us-gaap_SeriesCPreferredStockMember_09ea7c48-79c5-43f1-8fdf-6c378a1fc49d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesDPreferredStockMember_607a8deb-9afc-48b2-a4bb-583096aea2c5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesDPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_f54588e4-a230-4e09-a0a1-c6c252c898d2" xlink:to="loc_us-gaap_SeriesDPreferredStockMember_607a8deb-9afc-48b2-a4bb-583096aea2c5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesEPreferredStockMember_5b1c4e85-62d4-4098-bfe7-59ea09b3d107" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesEPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_f54588e4-a230-4e09-a0a1-c6c252c898d2" xlink:to="loc_us-gaap_SeriesEPreferredStockMember_5b1c4e85-62d4-4098-bfe7-59ea09b3d107" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_46b961d5-30e3-4f50-85fd-1b23c293ef23" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_f54588e4-a230-4e09-a0a1-c6c252c898d2" xlink:to="loc_us-gaap_CommonStockMember_46b961d5-30e3-4f50-85fd-1b23c293ef23" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockDividendsPerShareCashPaid_15a019e2-6867-44cf-8bc3-5e18648bb0b8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockDividendsPerShareCashPaid"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_b392d7a7-d2a5-4df4-8879-fd77aba5ec49" xlink:to="loc_us-gaap_PreferredStockDividendsPerShareCashPaid_15a019e2-6867-44cf-8bc3-5e18648bb0b8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockDividendsPerShareCashPaid_f0461aee-5088-4523-8e84-e8130cb5e43e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockDividendsPerShareCashPaid"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_b392d7a7-d2a5-4df4-8879-fd77aba5ec49" xlink:to="loc_us-gaap_CommonStockDividendsPerShareCashPaid_f0461aee-5088-4523-8e84-e8130cb5e43e" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.gladstoneland.com/role/SubsequentEventsAdditionalInformationDetails" xlink:type="simple" xlink:href="land-20240630.xsd#SubsequentEventsAdditionalInformationDetails"/>
  <link:definitionLink xlink:role="http://www.gladstoneland.com/role/SubsequentEventsAdditionalInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventLineItems_5872abb0-b504-4973-afd5-0ab8dca11bf4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTable_77317d94-c700-4b5e-ac20-65d748c8fd25" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SubsequentEventLineItems_5872abb0-b504-4973-afd5-0ab8dca11bf4" xlink:to="loc_us-gaap_SubsequentEventTable_77317d94-c700-4b5e-ac20-65d748c8fd25" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_c9e3d421-d5d1-4291-bb6e-396878f260e8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsequentEventTable_77317d94-c700-4b5e-ac20-65d748c8fd25" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_c9e3d421-d5d1-4291-bb6e-396878f260e8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_c9e3d421-d5d1-4291-bb6e-396878f260e8_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_c9e3d421-d5d1-4291-bb6e-396878f260e8" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_c9e3d421-d5d1-4291-bb6e-396878f260e8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_3fb27670-e5cf-44a8-9d2c-d31d8c44569a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_c9e3d421-d5d1-4291-bb6e-396878f260e8" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_3fb27670-e5cf-44a8-9d2c-d31d8c44569a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NotesPayableToBanksMember_d2e9bb4b-4447-4ff2-9c62-4c3285a4b418" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NotesPayableToBanksMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_3fb27670-e5cf-44a8-9d2c-d31d8c44569a" xlink:to="loc_us-gaap_NotesPayableToBanksMember_d2e9bb4b-4447-4ff2-9c62-4c3285a4b418" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeAxis_393d2826-25f3-40aa-a1a5-d082320d03d3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsequentEventTable_77317d94-c700-4b5e-ac20-65d748c8fd25" xlink:to="loc_us-gaap_SubsequentEventTypeAxis_393d2826-25f3-40aa-a1a5-d082320d03d3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_393d2826-25f3-40aa-a1a5-d082320d03d3_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_393d2826-25f3-40aa-a1a5-d082320d03d3" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_393d2826-25f3-40aa-a1a5-d082320d03d3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_07560545-49ab-4209-b937-e0e94e9e30ba" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_393d2826-25f3-40aa-a1a5-d082320d03d3" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_07560545-49ab-4209-b937-e0e94e9e30ba" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventMember_6a98c89b-c911-4ace-a317-4db1c35ddab2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventTypeDomain_07560545-49ab-4209-b937-e0e94e9e30ba" xlink:to="loc_us-gaap_SubsequentEventMember_6a98c89b-c911-4ace-a317-4db1c35ddab2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_cc4fb911-9d0c-4c00-9f9f-42675e37904e" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsequentEventTable_77317d94-c700-4b5e-ac20-65d748c8fd25" xlink:to="loc_srt_StatementGeographicalAxis_cc4fb911-9d0c-4c00-9f9f-42675e37904e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_cc4fb911-9d0c-4c00-9f9f-42675e37904e_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_cc4fb911-9d0c-4c00-9f9f-42675e37904e" xlink:to="loc_srt_SegmentGeographicalDomain_cc4fb911-9d0c-4c00-9f9f-42675e37904e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_a7a5764b-d50a-47b0-938c-5dd20ecffbf9" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_cc4fb911-9d0c-4c00-9f9f-42675e37904e" xlink:to="loc_srt_SegmentGeographicalDomain_a7a5764b-d50a-47b0-938c-5dd20ecffbf9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_ContraCostaCountyCAMember_5cfe7608-2e16-4655-85c0-5446fd937658" xlink:href="land-20240630.xsd#land_ContraCostaCountyCAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_a7a5764b-d50a-47b0-938c-5dd20ecffbf9" xlink:to="loc_land_ContraCostaCountyCAMember_5cfe7608-2e16-4655-85c0-5446fd937658" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CounterpartyNameAxis_c71cc0e3-7313-4261-bda2-a893edfe2df7" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_CounterpartyNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsequentEventTable_77317d94-c700-4b5e-ac20-65d748c8fd25" xlink:to="loc_srt_CounterpartyNameAxis_c71cc0e3-7313-4261-bda2-a893edfe2df7" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_c71cc0e3-7313-4261-bda2-a893edfe2df7_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CounterpartyNameAxis_c71cc0e3-7313-4261-bda2-a893edfe2df7" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_c71cc0e3-7313-4261-bda2-a893edfe2df7_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_86fdd2ac-78ac-47b5-b1e6-6a08d2767373" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CounterpartyNameAxis_c71cc0e3-7313-4261-bda2-a893edfe2df7" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_86fdd2ac-78ac-47b5-b1e6-6a08d2767373" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_ByronBethanyIrrigationDistrictMember_c4cefca6-89a4-4833-bb11-5f006e3ccb09" xlink:href="land-20240630.xsd#land_ByronBethanyIrrigationDistrictMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_86fdd2ac-78ac-47b5-b1e6-6a08d2767373" xlink:to="loc_land_ByronBethanyIrrigationDistrictMember_c4cefca6-89a4-4833-bb11-5f006e3ccb09" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_AreaOfWaterPurchased_bd63d98f-7881-473b-8e8a-02219dc13b0d" xlink:href="land-20240630.xsd#land_AreaOfWaterPurchased"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_5872abb0-b504-4973-afd5-0ab8dca11bf4" xlink:to="loc_land_AreaOfWaterPurchased_bd63d98f-7881-473b-8e8a-02219dc13b0d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_WaterAssetsPurchasedCarryingValue_9938107b-5a5e-403c-94bd-6a309fb03307" xlink:href="land-20240630.xsd#land_WaterAssetsPurchasedCarryingValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_5872abb0-b504-4973-afd5-0ab8dca11bf4" xlink:to="loc_land_WaterAssetsPurchasedCarryingValue_9938107b-5a5e-403c-94bd-6a309fb03307" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_AreaOfWaterAvailableForPurchaseContract_02498912-980b-4b7e-adac-d560858c6093" xlink:href="land-20240630.xsd#land_AreaOfWaterAvailableForPurchaseContract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_5872abb0-b504-4973-afd5-0ab8dca11bf4" xlink:to="loc_land_AreaOfWaterAvailableForPurchaseContract_02498912-980b-4b7e-adac-d560858c6093" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RepaymentsOfLongTermDebt_27f2478c-fb64-47e6-9c36-1eb651cfd731" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RepaymentsOfLongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_5872abb0-b504-4973-afd5-0ab8dca11bf4" xlink:to="loc_us-gaap_RepaymentsOfLongTermDebt_27f2478c-fb64-47e6-9c36-1eb651cfd731" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtWeightedAverageInterestRate_06e365a6-754c-498b-bc63-8acb5705ef75" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtWeightedAverageInterestRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_5872abb0-b504-4973-afd5-0ab8dca11bf4" xlink:to="loc_us-gaap_DebtWeightedAverageInterestRate_06e365a6-754c-498b-bc63-8acb5705ef75" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateEffectivePercentage_25044932-c021-4686-807b-3939f096a008" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentInterestRateEffectivePercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_5872abb0-b504-4973-afd5-0ab8dca11bf4" xlink:to="loc_us-gaap_DebtInstrumentInterestRateEffectivePercentage_25044932-c021-4686-807b-3939f096a008" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.gladstoneland.com/role/SubsequentEventsEquityIssuanceActivityDetails" xlink:type="simple" xlink:href="land-20240630.xsd#SubsequentEventsEquityIssuanceActivityDetails"/>
  <link:definitionLink xlink:role="http://www.gladstoneland.com/role/SubsequentEventsEquityIssuanceActivityDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventLineItems_2dbce9d6-066c-44fa-997d-a7568b9c92da" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTable_e5516fc4-e316-460a-85dd-26d181173970" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SubsequentEventLineItems_2dbce9d6-066c-44fa-997d-a7568b9c92da" xlink:to="loc_us-gaap_SubsequentEventTable_e5516fc4-e316-460a-85dd-26d181173970" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_86010559-e460-41ea-8e0e-031ce05ddf51" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsequentEventTable_e5516fc4-e316-460a-85dd-26d181173970" xlink:to="loc_us-gaap_StatementClassOfStockAxis_86010559-e460-41ea-8e0e-031ce05ddf51" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_86010559-e460-41ea-8e0e-031ce05ddf51_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_86010559-e460-41ea-8e0e-031ce05ddf51" xlink:to="loc_us-gaap_ClassOfStockDomain_86010559-e460-41ea-8e0e-031ce05ddf51_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_f1d45d44-73cb-4946-8a3e-7762caf6af73" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_86010559-e460-41ea-8e0e-031ce05ddf51" xlink:to="loc_us-gaap_ClassOfStockDomain_f1d45d44-73cb-4946-8a3e-7762caf6af73" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesEPreferredStockMember_9ffb4ab4-d8dc-417a-b575-c2a9f6629d8b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesEPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_f1d45d44-73cb-4946-8a3e-7762caf6af73" xlink:to="loc_us-gaap_SeriesEPreferredStockMember_9ffb4ab4-d8dc-417a-b575-c2a9f6629d8b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeAxis_b36db78f-29ec-4b90-b395-016c7f740d3a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsequentEventTable_e5516fc4-e316-460a-85dd-26d181173970" xlink:to="loc_us-gaap_SubsequentEventTypeAxis_b36db78f-29ec-4b90-b395-016c7f740d3a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_b36db78f-29ec-4b90-b395-016c7f740d3a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_b36db78f-29ec-4b90-b395-016c7f740d3a" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_b36db78f-29ec-4b90-b395-016c7f740d3a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_01629bf8-8d38-4149-84f7-ca9eace96e85" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_b36db78f-29ec-4b90-b395-016c7f740d3a" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_01629bf8-8d38-4149-84f7-ca9eace96e85" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventMember_165955ee-3e28-4c34-9211-ee847a89080c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventTypeDomain_01629bf8-8d38-4149-84f7-ca9eace96e85" xlink:to="loc_us-gaap_SubsequentEventMember_165955ee-3e28-4c34-9211-ee847a89080c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_7213976c-dd29-4f74-a6ad-551714ed1e21" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsequentEventTable_e5516fc4-e316-460a-85dd-26d181173970" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_7213976c-dd29-4f74-a6ad-551714ed1e21" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_7213976c-dd29-4f74-a6ad-551714ed1e21_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_7213976c-dd29-4f74-a6ad-551714ed1e21" xlink:to="loc_us-gaap_EquityComponentDomain_7213976c-dd29-4f74-a6ad-551714ed1e21_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_a359738c-909f-4783-9bcf-fc0582fd4883" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_7213976c-dd29-4f74-a6ad-551714ed1e21" xlink:to="loc_us-gaap_EquityComponentDomain_a359738c-909f-4783-9bcf-fc0582fd4883" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockMember_727127fa-c740-4508-b57b-7fac97957591" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_a359738c-909f-4783-9bcf-fc0582fd4883" xlink:to="loc_us-gaap_PreferredStockMember_727127fa-c740-4508-b57b-7fac97957591" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction_0aaec870-0c54-4375-8cd1-d69306deb721" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_2dbce9d6-066c-44fa-997d-a7568b9c92da" xlink:to="loc_us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction_0aaec870-0c54-4375-8cd1-d69306deb721" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_SaleOfStockWeightedAveragePricePerShare_0d10d151-d90f-4f96-8660-fe71437eb92f" xlink:href="land-20240630.xsd#land_SaleOfStockWeightedAveragePricePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_2dbce9d6-066c-44fa-997d-a7568b9c92da" xlink:to="loc_land_SaleOfStockWeightedAveragePricePerShare_0d10d151-d90f-4f96-8660-fe71437eb92f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOrSaleOfEquity_7ffac6c8-5f14-459f-b86a-ae4d9d4b46b4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromIssuanceOrSaleOfEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_2dbce9d6-066c-44fa-997d-a7568b9c92da" xlink:to="loc_us-gaap_ProceedsFromIssuanceOrSaleOfEquity_7ffac6c8-5f14-459f-b86a-ae4d9d4b46b4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockConsiderationReceivedOnTransaction_c4c60f3f-bd1b-4b3f-a04b-d2c950bb3dc7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SaleOfStockConsiderationReceivedOnTransaction"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_2dbce9d6-066c-44fa-997d-a7568b9c92da" xlink:to="loc_us-gaap_SaleOfStockConsiderationReceivedOnTransaction_c4c60f3f-bd1b-4b3f-a04b-d2c950bb3dc7" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.gladstoneland.com/role/SubsequentEventsEquityRepurchaseActivityDetails" xlink:type="simple" xlink:href="land-20240630.xsd#SubsequentEventsEquityRepurchaseActivityDetails"/>
  <link:definitionLink xlink:role="http://www.gladstoneland.com/role/SubsequentEventsEquityRepurchaseActivityDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventLineItems_8e0686b4-5200-4ecb-8dc7-b50c94f1e385" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTable_fb7327fb-7f8a-4638-8ad6-95529f676812" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SubsequentEventLineItems_8e0686b4-5200-4ecb-8dc7-b50c94f1e385" xlink:to="loc_us-gaap_SubsequentEventTable_fb7327fb-7f8a-4638-8ad6-95529f676812" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_e7233e90-f087-455d-b8d5-372d9c4410f6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsequentEventTable_fb7327fb-7f8a-4638-8ad6-95529f676812" xlink:to="loc_us-gaap_StatementClassOfStockAxis_e7233e90-f087-455d-b8d5-372d9c4410f6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_e7233e90-f087-455d-b8d5-372d9c4410f6_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_e7233e90-f087-455d-b8d5-372d9c4410f6" xlink:to="loc_us-gaap_ClassOfStockDomain_e7233e90-f087-455d-b8d5-372d9c4410f6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_1d18955c-e749-4c6f-ab68-19fe3f2da6ff" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_e7233e90-f087-455d-b8d5-372d9c4410f6" xlink:to="loc_us-gaap_ClassOfStockDomain_1d18955c-e749-4c6f-ab68-19fe3f2da6ff" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesBPreferredStockMember_3461195c-32f2-49d8-97fa-acebbfe1e6e8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesBPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_1d18955c-e749-4c6f-ab68-19fe3f2da6ff" xlink:to="loc_us-gaap_SeriesBPreferredStockMember_3461195c-32f2-49d8-97fa-acebbfe1e6e8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesCPreferredStockMember_dfe54d0c-c410-40d6-87c7-30cd6c5f6504" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesCPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_1d18955c-e749-4c6f-ab68-19fe3f2da6ff" xlink:to="loc_us-gaap_SeriesCPreferredStockMember_dfe54d0c-c410-40d6-87c7-30cd6c5f6504" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_baa31979-e245-4851-b597-46925728a5d7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsequentEventTable_fb7327fb-7f8a-4638-8ad6-95529f676812" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_baa31979-e245-4851-b597-46925728a5d7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_baa31979-e245-4851-b597-46925728a5d7_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_baa31979-e245-4851-b597-46925728a5d7" xlink:to="loc_us-gaap_EquityComponentDomain_baa31979-e245-4851-b597-46925728a5d7_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_8e204d25-db46-4b55-8706-f2e2d4f6cbc4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_baa31979-e245-4851-b597-46925728a5d7" xlink:to="loc_us-gaap_EquityComponentDomain_8e204d25-db46-4b55-8706-f2e2d4f6cbc4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockMember_2b3618d3-b1c7-4663-9a79-c2cd389062ca" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_8e204d25-db46-4b55-8706-f2e2d4f6cbc4" xlink:to="loc_us-gaap_PreferredStockMember_2b3618d3-b1c7-4663-9a79-c2cd389062ca" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeAxis_43574dea-ac97-4b42-ad68-9fac3f650d5a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsequentEventTable_fb7327fb-7f8a-4638-8ad6-95529f676812" xlink:to="loc_us-gaap_SubsequentEventTypeAxis_43574dea-ac97-4b42-ad68-9fac3f650d5a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_43574dea-ac97-4b42-ad68-9fac3f650d5a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_43574dea-ac97-4b42-ad68-9fac3f650d5a" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_43574dea-ac97-4b42-ad68-9fac3f650d5a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_2d7892db-17bd-4eff-801e-d09e274f5085" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_43574dea-ac97-4b42-ad68-9fac3f650d5a" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_2d7892db-17bd-4eff-801e-d09e274f5085" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventMember_434ded84-d94f-48ee-bb03-725234191cdc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventTypeDomain_2d7892db-17bd-4eff-801e-d09e274f5085" xlink:to="loc_us-gaap_SubsequentEventMember_434ded84-d94f-48ee-bb03-725234191cdc" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockRepurchasedDuringPeriodShares_0839fc67-2f7e-421b-aec4-a7d6dacedbb1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockRepurchasedDuringPeriodShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_8e0686b4-5200-4ecb-8dc7-b50c94f1e385" xlink:to="loc_us-gaap_StockRepurchasedDuringPeriodShares_0839fc67-2f7e-421b-aec4-a7d6dacedbb1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockRepurchasedDuringPeriodValue_90b14e39-db32-4e7b-a09b-c74f5f28508c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockRepurchasedDuringPeriodValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_8e0686b4-5200-4ecb-8dc7-b50c94f1e385" xlink:to="loc_us-gaap_StockRepurchasedDuringPeriodValue_90b14e39-db32-4e7b-a09b-c74f5f28508c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockAcquiredAverageCostPerShare_be0f872d-a43c-4753-a124-61149df9d694" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_TreasuryStockAcquiredAverageCostPerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_8e0686b4-5200-4ecb-8dc7-b50c94f1e385" xlink:to="loc_us-gaap_TreasuryStockAcquiredAverageCostPerShare_be0f872d-a43c-4753-a124-61149df9d694" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherPreferredStockDividendsAndAdjustments_1df1cbe3-88a7-47a7-9b87-78ff5ba445f3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherPreferredStockDividendsAndAdjustments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_8e0686b4-5200-4ecb-8dc7-b50c94f1e385" xlink:to="loc_us-gaap_OtherPreferredStockDividendsAndAdjustments_1df1cbe3-88a7-47a7-9b87-78ff5ba445f3" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.gladstoneland.com/role/SubsequentEventsScheduleofDividendsDeclaredDetails" xlink:type="simple" xlink:href="land-20240630.xsd#SubsequentEventsScheduleofDividendsDeclaredDetails"/>
  <link:definitionLink xlink:role="http://www.gladstoneland.com/role/SubsequentEventsScheduleofDividendsDeclaredDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventLineItems_6bebb1d4-1217-48b5-8260-21c580879703" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTable_a3c46230-bc30-43e0-87fd-fbe555848d9c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SubsequentEventLineItems_6bebb1d4-1217-48b5-8260-21c580879703" xlink:to="loc_us-gaap_SubsequentEventTable_a3c46230-bc30-43e0-87fd-fbe555848d9c" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_34de492f-40fd-4e42-ac44-d59d8678465c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsequentEventTable_a3c46230-bc30-43e0-87fd-fbe555848d9c" xlink:to="loc_us-gaap_StatementClassOfStockAxis_34de492f-40fd-4e42-ac44-d59d8678465c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_34de492f-40fd-4e42-ac44-d59d8678465c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_34de492f-40fd-4e42-ac44-d59d8678465c" xlink:to="loc_us-gaap_ClassOfStockDomain_34de492f-40fd-4e42-ac44-d59d8678465c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_06e3201d-47c5-412e-9abb-1b8b9328e002" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_34de492f-40fd-4e42-ac44-d59d8678465c" xlink:to="loc_us-gaap_ClassOfStockDomain_06e3201d-47c5-412e-9abb-1b8b9328e002" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesBPreferredStockMember_9a7ea3e0-d1e0-44cf-856a-4d219cc9cc27" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesBPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_06e3201d-47c5-412e-9abb-1b8b9328e002" xlink:to="loc_us-gaap_SeriesBPreferredStockMember_9a7ea3e0-d1e0-44cf-856a-4d219cc9cc27" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesCPreferredStockMember_d19b99f2-e958-4fe4-9862-716d57069959" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesCPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_06e3201d-47c5-412e-9abb-1b8b9328e002" xlink:to="loc_us-gaap_SeriesCPreferredStockMember_d19b99f2-e958-4fe4-9862-716d57069959" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesDPreferredStockMember_e78d40d9-aaf5-4d39-89df-8b8511575a75" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesDPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_06e3201d-47c5-412e-9abb-1b8b9328e002" xlink:to="loc_us-gaap_SeriesDPreferredStockMember_e78d40d9-aaf5-4d39-89df-8b8511575a75" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeriesEPreferredStockMember_7ff2b7a4-0989-43dd-839a-5140642b69c8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SeriesEPreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_06e3201d-47c5-412e-9abb-1b8b9328e002" xlink:to="loc_us-gaap_SeriesEPreferredStockMember_7ff2b7a4-0989-43dd-839a-5140642b69c8" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_e5342ebe-af9a-4039-a3ee-6d08334ac928" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_06e3201d-47c5-412e-9abb-1b8b9328e002" xlink:to="loc_us-gaap_CommonStockMember_e5342ebe-af9a-4039-a3ee-6d08334ac928" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_DividendDeclaredBatchAxis_93245745-f326-479a-9072-a80b2f4c2585" xlink:href="land-20240630.xsd#land_DividendDeclaredBatchAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsequentEventTable_a3c46230-bc30-43e0-87fd-fbe555848d9c" xlink:to="loc_land_DividendDeclaredBatchAxis_93245745-f326-479a-9072-a80b2f4c2585" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_DividendDeclaredBatchDomain_93245745-f326-479a-9072-a80b2f4c2585_default" xlink:href="land-20240630.xsd#land_DividendDeclaredBatchDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_land_DividendDeclaredBatchAxis_93245745-f326-479a-9072-a80b2f4c2585" xlink:to="loc_land_DividendDeclaredBatchDomain_93245745-f326-479a-9072-a80b2f4c2585_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_DividendDeclaredBatchDomain_265ad5f1-2c38-45bb-bba2-73ff2398c6de" xlink:href="land-20240630.xsd#land_DividendDeclaredBatchDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_land_DividendDeclaredBatchAxis_93245745-f326-479a-9072-a80b2f4c2585" xlink:to="loc_land_DividendDeclaredBatchDomain_265ad5f1-2c38-45bb-bba2-73ff2398c6de" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_DividendDeclaredBatchOneMember_4d7e194c-fcbd-4dd2-aebb-059e9a7f6430" xlink:href="land-20240630.xsd#land_DividendDeclaredBatchOneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_land_DividendDeclaredBatchDomain_265ad5f1-2c38-45bb-bba2-73ff2398c6de" xlink:to="loc_land_DividendDeclaredBatchOneMember_4d7e194c-fcbd-4dd2-aebb-059e9a7f6430" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_DividendDeclaredBatchTwoMember_d58ef3ca-72f6-4d54-8540-703ef68beb52" xlink:href="land-20240630.xsd#land_DividendDeclaredBatchTwoMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_land_DividendDeclaredBatchDomain_265ad5f1-2c38-45bb-bba2-73ff2398c6de" xlink:to="loc_land_DividendDeclaredBatchTwoMember_d58ef3ca-72f6-4d54-8540-703ef68beb52" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_land_DividendDeclaredBatchThreeMember_7d02d2a7-daf7-44fe-b7a5-35211a1e9991" xlink:href="land-20240630.xsd#land_DividendDeclaredBatchThreeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_land_DividendDeclaredBatchDomain_265ad5f1-2c38-45bb-bba2-73ff2398c6de" xlink:to="loc_land_DividendDeclaredBatchThreeMember_7d02d2a7-daf7-44fe-b7a5-35211a1e9991" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeAxis_614928ef-db54-4f37-b2e4-daa5dfb5aa6f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsequentEventTable_a3c46230-bc30-43e0-87fd-fbe555848d9c" xlink:to="loc_us-gaap_SubsequentEventTypeAxis_614928ef-db54-4f37-b2e4-daa5dfb5aa6f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_614928ef-db54-4f37-b2e4-daa5dfb5aa6f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_614928ef-db54-4f37-b2e4-daa5dfb5aa6f" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_614928ef-db54-4f37-b2e4-daa5dfb5aa6f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_b7377259-4ba3-4d25-a3e5-1c9216ee2f10" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_614928ef-db54-4f37-b2e4-daa5dfb5aa6f" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_b7377259-4ba3-4d25-a3e5-1c9216ee2f10" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventMember_62897174-543c-4619-bbe1-ea64432acd03" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SubsequentEventMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventTypeDomain_b7377259-4ba3-4d25-a3e5-1c9216ee2f10" xlink:to="loc_us-gaap_SubsequentEventMember_62897174-543c-4619-bbe1-ea64432acd03" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockDividendsPerShareDeclared_417cedea-a7dd-4572-ae86-e273d6131ede" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PreferredStockDividendsPerShareDeclared"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_6bebb1d4-1217-48b5-8260-21c580879703" xlink:to="loc_us-gaap_PreferredStockDividendsPerShareDeclared_417cedea-a7dd-4572-ae86-e273d6131ede" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockDividendsPerShareDeclared_2c7f59c1-4f08-4613-84f8-06a26cb9ae80" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockDividendsPerShareDeclared"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_6bebb1d4-1217-48b5-8260-21c580879703" xlink:to="loc_us-gaap_CommonStockDividendsPerShareDeclared_2c7f59c1-4f08-4613-84f8-06a26cb9ae80" xlink:type="arc" order="1"/>
  </link:definitionLink>
</link:linkbase>
