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Consolidated Statements of Cash Flows (Unaudited) - USD ($) $ in Thousands | 12 Months Ended
--- | ---
Dec. 31, 2020 | Dec. 31, 2019 | Dec. 31, 2018
Cash flows from operating activities: |  |  |
Net loss | $ (190,228) | $ (105,385) | $ (52,248)
Adjustments to reconcile net loss to net cash used in operating activities: |  |  |
Depreciation and amortization | 5,870 | 3,775 | 1,019
Stock-based compensation | 30,522 | 16,281 | 6,925
Amortization of premiums and accretion of discounts on marketable securities | 584 | (4,701) | (2,745)
Amortization of right-of-use assets | 1,395 | 1,799 |
Impairment loss on right-of-use assets | 238 | 1,158 |
Loss from disposal of property and equipment, net | 107 | 39 | 151
Changes in operating assets and liabilities: |  |  |
Prepaid expenses and other current assets | (3,839) | (1,596) | (2,245)
Other assets | (2,498) | (42) | 106
Accounts payable | 2,409 | 152 | (809)
Accrued liabilities and accrued clinical supply costs | 12,232 | 9,210 | 4,743
Deferred revenue | (21,098) | (21,219) | 172,492
Lease liabilities | (2,407) | 868 |
Other long-term liabilities | (21) | 353 | 75
Net cash provided by (used in) operating activities | (166,734) | (99,308) | 127,464
Cash flows from investing activities: |  |  |
Purchase of property and equipment | (5,032) | (15,265) | (1,884)
Purchase of marketable securities | (506,809) | (529,609) | (472,235)
Maturities of marketable securities | 406,790 | 496,000 | 250,000
Net cash used in investing activities | (105,051) | (48,874) | (224,119)
Cash flows from financing activities: |  |  |
Proceeds from issuance of common stock upon initial public offering, net of issuance costs |  | 170,128 | (1,904)
Proceeds from issuance of common stock upon follow-on public offering, net of issuance cost | 224,603 | (92) |
Proceeds from issuance of convertible preferred stock, net of issuance costs |  |  | 133,050
Proceeds from the exercise of options to purchase common stock | 6,331 | 1,519 |
Proceeds from issuance of stock from employee stock purchase plan | 1,179 | 798 |
Net cash provided by financing activities | 232,113 | 172,353 | 131,146
Net increase (decrease) in cash, cash equivalents, and restricted cash | (39,672) | 24,171 | 34,491
Cash, cash equivalents, and restricted cash at beginning of period | 91,113 | 66,942 | 32,451
Cash, cash equivalents, and restricted cash at end of period | 51,441 | 91,113 | 66,942
Non-cash investing and financing activities: |  |  |
Property and equipment purchases included in accounts payable and accrued liabilities | $ 428 | 3,471 | 293
Deferred offering costs in accrued liabilities |  | 465 | 792
Tenant improvements paid by landlord |  | $ 8,286 | $ 7,449

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