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CONSOLIDATED STATEMENTS OF CASH FLOWS - USD ($) $ in Thousands | 12 Months Ended
--- | ---
Dec. 31, 2021 | Dec. 31, 2020 | Dec. 31, 2019
Operating activities |  |  |
Net loss | $ (202,115) | $ (129,739) | $ (151,811)
Adjustments to reconcile net loss to net cash provided by (used in) operating activities: |  |  |
Depreciation and amortization | 11,258 | 11,957 | 12,306
Amortization of premiums and discounts on marketable securities | 1,607 | (260) | (1,461)
Share-based compensation | 23,126 | 20,676 | 19,571
Other non-cash items | 2,035 | 0 | 0
Changes in operating assets and liabilities: |  |  |
Accounts receivable | 12,696 | (10,337) | 16,839
Inventory | (6,424) | 0 | 0
Prepaid expenses and other current assets | (4,188) | (5,697) | (4,878)
Other non current assets | 5,915 | 581 | (1,578)
Accounts payable | 7,125 | 3,723 | 787
Accrued expenses and other current liabilities | (607) | 6,994 | (6,057)
Lease liabilities | (3,780) | (1,324) | 2,881
Deferred revenue | 9,264 | (8,472) | (20,869)
Other non current liabilities | 258 | 0 | 0
Net Cash Provided by (Used in) Operating Activities, Total | (143,830) | (111,898) | (134,270)
Cash flows from investing activities |  |  |
Purchases of marketable securities | (231,208) | (223,745) | (264,399)
Proceeds from sales and maturities of marketable securities | 200,800 | 221,866 | 189,330
Purchases of property, equipment and software | (6,201) | (5,906) | (4,289)
Net Cash Provided by (Used in) Investing Activities, Total | (36,609) | (7,785) | (79,358)
Cash flows from financing activities |  |  |
Proceeds from issuance of common stock, net of offering costs | 117,818 | 170,456 | 118,657
Proceeds from stock option exercises and ESPP purchases | 4,959 | 3,886 | 1,315
Net Cash Provided by (Used in) Financing Activities, Total | 122,777 | 174,342 | 119,972
Net change in cash and cash equivalents | (57,662) | 54,659 | (93,656)
Cash and cash equivalents at beginning of period | 181,131 | 126,472 | 220,128
Cash and cash equivalents at end of period | 123,469 | 181,131 | 126,472
Non-cash operating and investing activities |  |  |
Right-of-use assets modified in exchange for operating lease obligation | 0 | 0 | 6,408
Capital Expenditures Incurred but Not yet Paid | $ 508 | $ 66 | $ 66

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