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Intangible Assets | 3 Months Ended | 12 Months Ended
--- | --- | ---
Mar. 31, 2022 | Dec. 31, 2021
Intangible Assets |  |
Intangible Assets | Note 5 – Intangible Assets Intangible assets are recorded at cost and consist of the license agreement with Duke University. The Company issued Duke University a small block of shares of common stock estimated to have a fair value of $1,073,529 as consideration for granting the Company the license based on the Company’s common stock market price on the date the license agreement was executed (see Note 8). Intangible assets are comprised of the following as of March 31, 2022 and December 31, 2021:
Name |  | Estimated Life |  | Balance at December 31, 2021 |  |  | Additions |  |  | Amortization |  |  | Balance at March 31, 2022 |
License agreement |  | 17 Years |  | $ | 1,028,114 |  |  | $ | — |  |  | $ | 15,787 |  |  | $ | 1,012,327 |
Patents |  | 20 Years |  |  | 34,742 |  |  |  | — |  |  |  | 436 |  |  |  | 34,306 |
Total |  |  |  | $ | 1,062,856 |  |  | $ | — |  |  | $ | 16,223 |  |  | $ | 1,046,633 |
Depreciation and Amortization expense for the three months ended March 31, 2022, was $16,458. Estimated future amortization expense as of March 31, 2022:
|  | March 31, |
|  | 2022 |
2022 (Remaining 9 months) |  | $ | 48,663 |
2023 |  |  | 64,884 |
2024 |  |  | 64,884 |
2025 |  |  | 64,884 |
2026 |  |  | 64,884 |
Thereafter |  |  | 738,434 |
Intangible assets, Net |  | $ | 1,046,633 |
| Note 5 – Intangible Assets Intangible assets are recorded at cost and consist of the license agreement with Duke University. The Company issued Duke University a small block of shares of common stock estimated to have a fair value of $1,073,529 as consideration for granting the Company the license based on the Company’s common stock market price on the date the license agreement was executed (see Note 8). Intangible assets are comprised of the following as of December 31, 2021 and 2020:
Name |  | Estimated Life |  | Balance at December 31, 2020 |  |  | Additions |  |  | Amortization |  |  | Balance at December 31, 2021 |
License agreement |  | 17 Years |  | $ | - |  |  | $ | 1,073,529 |  |  | $ | 45,415 |  |  | $ | 1,028,114 |
Patents |  | 20 Years |  |  | - |  |  |  | 34,741 |  |  |  | - |  |  |  | 34,741 |
Total |  |  |  | $ | - |  |  | $ | 1,108,270 |  |  | $ | 45,415 |  |  | $ | 1,062,855 |
Amortization expense for the year ended December 31, 2021 was $45,415. There is no amortization expense associated with the patent expenses. Estimated future amortization expense as of December 31, 2021:
|  | December 31, |
|  | 2021 |
2022 |  | $ | 63,149 |
2023 |  |  | 63,149 |
2024 |  |  | 63,149 |
2025 |  |  | 63,149 |
2026 |  |  | 63,149 |
Thereafter |  |  | 712,369 |
Intangible assets, Net |  | $ | 1,028,114 |

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- Definition The entire disclosure for all or part of the information related to intangible assets. + References Reference 1: http://fasb.org/us-gaap/role/ref/legacyRef -Publisher FASB -Name Accounting Standards Codification -Topic 350 -URI http://asc.fasb.org/topic&trid=2144416 + Details
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