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Consolidated Statements of Cash Flows - USD ($) | 12 Months Ended
--- | ---
Dec. 31, 2020 | Dec. 31, 2019 | Dec. 31, 2018
Cash flows from operating activities |  |  |
Net loss | $ (9,325,273) | $ (8,307,965) |
Adjustments to reconcile net loss to net cash used in operating activities: |  |  |
Stock-based compensation expense | 1,651,893 | 1,171,079 |
Amortization of held-to-maturity securities, net | 331,277 | 198,208 |
Unrealized gain from investment in Alphazyme | (284,709) | 0 |
Foreign currency exchange loss (gain), net | 62,345 | 27,725 |
Changes in operating assets and liabilities: |  |  |
Interest receivable | 217,464 | (35,471) |
Accounts receivable | 363,365 | (252,772) |
Income tax receivable | 500,616 | 506,866 |
Prepaid research and development | 0 | 253,446 |
Prepaid expenses and other current assets | (2,410) | (105,707) |
Accounts payable | (53,200) | 657,658 |
Accrued expenses | (80,132) | 166,399 |
Deferred research and development obligations | 44,372 | (62,358) |
Net cash used in operating activities | (6,574,392) | (5,782,892) |
Cash flows from investing activities |  |  |
Purchases of held-to-maturity investment securities | (17,638,947) | (47,615,550) |
Proceeds from maturities of investment securities | 39,761,000 | 55,323,000 |
Net cash provided by investing activities | 22,122,053 | 7,707,450 |
Cash flows from financing activities |  |  |
Proceeds from exercise of options | 255,470 | 550,188 |
Net cash provided by (used in) financing activities | 255,470 | 550,188 |
Effect of exchange rate changes on cash | 10,370 | (37,516) |
Net decrease in cash and cash equivalents | 15,813,501 | 2,437,230 |
Cash and cash equivalents at beginning of period | 4,823,544 | 2,386,314 |
Cash and cash equivalents at end of period | 20,637,045 | 4,823,544 | $ 2,386,314
Supplemental cash flow information |  |  |
Cash received from income tax refund | $ 500,616 | $ 506,866 | $ 500,000

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- References No definition available. + Details
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- Definition Amount of unrealized gain (loss) on investment. + References Reference 1: http://fasb.org/us-gaap/role/ref/legacyRef -Publisher FASB -Name Accounting Standards Codification -Topic 230 -SubTopic 10 -Section 45 -Paragraph 28 -Subparagraph (b) -URI http://asc.fasb.org/extlink&oid=121586364&loc=d3e3602-108585 + Details
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