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Statements of Changes in Stockholders' Equity (Deficit) - USD ($) | Common Stock | Additional Paid-in Capital | Accumulated Deficit | Total
--- | --- | --- | --- | ---
Balance at Dec. 31, 2023 | $ 1,052 | $ 102,507,189 | $ (110,259,087) | $ (7,750,846)
Balance, shares at Dec. 31, 2023 | 10,519,933 |  |  |
Changes in Stockholders' Equity (Deficit) |  |  |  |
Exercise of common stock warrants, inclusive of warrant reduction | $ 6 | 1,223,994 |  | 1,224,000
Exercise of common stock warrants, inclusive of warrant reduction, shares | 60,000 |  |  |
Issuance of common stock, net of issuance costs | $ 43 | 3,874,957 |  | 3,875,000
Issuance of common stock, net of issuance costs, shares | 431,366 |  |  |
Stock-based compensation expense |  | 364,765 |  | 364,765
Net loss |  |  | (1,066,947) | (1,066,947)
Balance at Mar. 31, 2024 | $ 1,101 | 107,970,905 | (111,326,034) | (3,354,028)
Balance, shares at Mar. 31, 2024 | 11,011,299 |  |  |
Balance at Dec. 31, 2023 | $ 1,052 | 102,507,189 | (110,259,087) | (7,750,846)
Balance, shares at Dec. 31, 2023 | 10,519,933 |  |  |
Changes in Stockholders' Equity (Deficit) |  |  |  |
Net loss |  |  |  | (6,087,359)
Balance at Jun. 30, 2024 | $ 1,172 | 114,594,390 | (116,346,446) | (1,750,884)
Balance, shares at Jun. 30, 2024 | 11,721,480 |  |  |
Balance at Mar. 31, 2024 | $ 1,101 | 107,970,905 | (111,326,034) | (3,354,028)
Balance, shares at Mar. 31, 2024 | 11,011,299 |  |  |
Changes in Stockholders' Equity (Deficit) |  |  |  |
Stock-based compensation expense |  | 1,596,512 |  | 1,596,512
Net loss |  |  | (5,020,412) | (5,020,412)
Issuance of common stock pursuant to ELOC, net of issuance costs | $ 71 | 5,026,973 |  | 5,027,044
Issuance of common stock pursuant to ELOC, net of issuance costs, shares | 710,181 |  |  |
Balance at Jun. 30, 2024 | $ 1,172 | 114,594,390 | (116,346,446) | (1,750,884)
Balance, shares at Jun. 30, 2024 | 11,721,480 |  |  |
Balance at Dec. 31, 2024 | $ 1,414 | 144,155,694 | (134,849,462) | 9,307,646
Balance, shares at Dec. 31, 2024 | 14,141,521 |  |  |
Changes in Stockholders' Equity (Deficit) |  |  |  |
Issuance of common stock pursuant to Equity Distribution Agreement, net of issuance costs | $ 9 | 502,925 |  | 502,934
Issuance of common stock pursuant to Equity Distribution Agreement, net of issuance costs, shares | 94,710 |  |  |
Issuance of common stock and warrants under registered direct offering, net of issuance costs | $ 525 | 19,279,964 |  | 19,280,489
Issuance of common stock and warrants under registered direct offering, net of issuance costs, shares | 5,250,000 |  |  |
Stock-based compensation expense |  | 499,887 |  | 499,887
Net loss |  |  | (5,537,069) | (5,537,069)
Balance at Mar. 31, 2025 | $ 1,948 | 164,438,470 | (140,386,531) | 24,053,887
Balance, shares at Mar. 31, 2025 | 19,486,231 |  |  |
Balance at Dec. 31, 2024 | $ 1,414 | 144,155,694 | (134,849,462) | 9,307,646
Balance, shares at Dec. 31, 2024 | 14,141,521 |  |  |
Changes in Stockholders' Equity (Deficit) |  |  |  |
Net loss |  |  |  | (11,757,127)
Balance at Jun. 30, 2025 | $ 1,948 | 164,935,088 | (146,606,589) | 18,330,447
Balance, shares at Jun. 30, 2025 | 19,486,231 |  |  |
Balance at Mar. 31, 2025 | $ 1,948 | 164,438,470 | (140,386,531) | 24,053,887
Balance, shares at Mar. 31, 2025 | 19,486,231 |  |  |
Changes in Stockholders' Equity (Deficit) |  |  |  |
Stock-based compensation expense |  | 496,618 |  | 496,618
Net loss |  |  | (6,220,058) | (6,220,058)
Balance at Jun. 30, 2025 | $ 1,948 | $ 164,935,088 | $ (146,606,589) | $ 18,330,447
Balance, shares at Jun. 30, 2025 | 19,486,231 |  |  |

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