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        <link:usedOn>link:definitionLink</link:usedOn>

        <link:usedOn>link:calculationLink</link:usedOn>
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        <link:usedOn>link:definitionLink</link:usedOn>

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        <link:usedOn>link:definitionLink</link:usedOn>

        <link:usedOn>link:calculationLink</link:usedOn>
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        <link:usedOn>link:definitionLink</link:usedOn>

        <link:usedOn>link:calculationLink</link:usedOn>
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  <element name="WarrantsIssuedToPurchaseOfCommonStock" type="xbrli:sharesItemType" abstract="false" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_WarrantsIssuedToPurchaseOfCommonStock" />

  <element name="WarrantsExercisePricePerShare" type="num:perShareItemType" abstract="false" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_WarrantsExercisePricePerShare" />

  <element name="WarrantsExercisableTerm" type="xbrli:durationItemType" abstract="false" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_WarrantsExercisableTerm" />

  <element name="TwoThousandThirteenStockOptionPlanMember" type="nonnum:domainItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_TwoThousandThirteenStockOptionPlanMember" />

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  <element name="PaymentOfContractualObligations" type="xbrli:monetaryItemType" abstract="false" xbrli:periodType="duration" nillable="true" xbrli:balance="credit" substitutionGroup="xbrli:item" id="aytu_PaymentOfContractualObligations" />

  <element name="ShareBasedCompensationShareWarrantsIssuedOnConvertiblePromisoryNotes" type="xbrli:sharesItemType" abstract="false" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_ShareBasedCompensationShareWarrantsIssuedOnConvertiblePromisoryNotes" />

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  <element name="EstimatedSalesReturnsAndAllowancesPolicyTextBlock" type="nonnum:textBlockItemType" abstract="false" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_EstimatedSalesReturnsAndAllowancesPolicyTextBlock" />

  <element name="NatestoBusinessPurchaseIncludedInNatestoPayable" type="xbrli:monetaryItemType" abstract="false" xbrli:periodType="duration" nillable="true" xbrli:balance="credit" substitutionGroup="xbrli:item" id="aytu_NatestoBusinessPurchaseIncludedInNatestoPayable" />

  <element name="WarrantDerivativeLiabilityToIssuanceOfRegisteredOfferingPlacementAgentWarrants" type="xbrli:monetaryItemType" abstract="false" xbrli:periodType="duration" nillable="true" xbrli:balance="credit" substitutionGroup="xbrli:item" id="aytu_WarrantDerivativeLiabilityToIssuanceOfRegisteredOfferingPlacementAgentWarrants" />

  <element name="LicenseAgreementTextBlock" type="nonnum:textBlockItemType" abstract="false" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_LicenseAgreementTextBlock" />

  <element name="StockAndIssuedDuringPeriodshares" type="xbrli:sharesItemType" abstract="false" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_StockAndIssuedDuringPeriodshares" />

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  <element name="NatestoMember" type="nonnum:domainItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_NatestoMember" />

  <element name="ManagementFeeMember" type="nonnum:domainItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_ManagementFeeMember" />

  <element name="MonthlyReductionInContractualObligation" type="xbrli:monetaryItemType" abstract="false" xbrli:periodType="duration" nillable="true" xbrli:balance="credit" substitutionGroup="xbrli:item" id="aytu_MonthlyReductionInContractualObligation" />

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  <element name="ReverseMergerShare" type="xbrli:sharesItemType" abstract="false" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_ReverseMergerShare" />

  <element name="AdjustmentForRoundingOfValueDueToConversion" type="xbrli:monetaryItemType" abstract="false" xbrli:periodType="duration" nillable="true" xbrli:balance="credit" substitutionGroup="xbrli:item" id="aytu_AdjustmentForRoundingOfValueDueToConversion" />

  <element name="SecondUpfrontMember" type="nonnum:domainItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_SecondUpfrontMember" />

  <element name="AdjustmentForRoundingOfSharesDueToConversion" type="xbrli:sharesItemType" abstract="false" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_AdjustmentForRoundingOfSharesDueToConversion" />

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  <element name="ThirdUpfrontMember" type="nonnum:domainItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_ThirdUpfrontMember" />

  <element name="OneTimeNonrefundablePayments" type="xbrli:monetaryItemType" abstract="false" xbrli:periodType="duration" nillable="true" xbrli:balance="debit" substitutionGroup="xbrli:item" id="aytu_OneTimeNonrefundablePayments" />

  <element name="SalesRevenueTarget" type="xbrli:monetaryItemType" abstract="false" xbrli:periodType="duration" nillable="true" xbrli:balance="credit" substitutionGroup="xbrli:item" id="aytu_SalesRevenueTarget" />

  <element name="FirstMilestoneMember" type="nonnum:domainItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_FirstMilestoneMember" />

  <element name="SecondMilestoneMember" type="nonnum:domainItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_SecondMilestoneMember" />

  <element name="ThirdMilestoneMember" type="nonnum:domainItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_ThirdMilestoneMember" />

  <element name="ForthMilestoneMember" type="nonnum:domainItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_ForthMilestoneMember" />

  <element name="FifthMilestoneMember" type="nonnum:domainItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_FifthMilestoneMember" />

  <element name="LicenseAndSupplyAgreementMember" type="nonnum:domainItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_LicenseAndSupplyAgreementMember" />

  <element name="SharePurchaseAgreementMaximumAmountOfSharesToBePurchased" type="xbrli:monetaryItemType" abstract="false" xbrli:periodType="instant" nillable="true" xbrli:balance="credit" substitutionGroup="xbrli:item" id="aytu_SharePurchaseAgreementMaximumAmountOfSharesToBePurchased" />

  <element name="AdditionalPaymentsForAdvisoryServices" type="xbrli:monetaryItemType" abstract="false" xbrli:periodType="instant" nillable="true" xbrli:balance="credit" substitutionGroup="xbrli:item" id="aytu_AdditionalPaymentsForAdvisoryServices" />

  <element name="ExecutivesEmployeesDirectorsAndConsultantsMember" type="nonnum:domainItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_ExecutivesEmployeesDirectorsAndConsultantsMember" />

  <element name="LincolnParkCapitalFundLlcMember" type="nonnum:domainItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_LincolnParkCapitalFundLlcMember" />

  <element name="JosephGunnarCoLlcMember" type="nonnum:domainItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_JosephGunnarCoLlcMember" />

  <element name="FordhamFinancialManagementIncMember" type="nonnum:domainItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_FordhamFinancialManagementIncMember" />

  <element name="BusinessDescriptionAndBasisOfPresentationTable" type="xbrli:stringItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrldt:hypercubeItem" id="aytu_BusinessDescriptionAndBasisOfPresentationTable" />

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  <element name="PotentialMilestonePaymentsReceivable" type="xbrli:monetaryItemType" abstract="false" xbrli:periodType="instant" nillable="true" xbrli:balance="debit" substitutionGroup="xbrli:item" id="aytu_PotentialMilestonePaymentsReceivable" />

  <element name="PercentageOfRoyaltyRateOfNetSales" type="num:percentItemType" abstract="false" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_PercentageOfRoyaltyRateOfNetSales" />

  <element name="DeferredRevenueRecognitionPeriod" type="xbrli:durationItemType" abstract="false" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_DeferredRevenueRecognitionPeriod" />

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  <element name="SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsForfeitureRate" type="num:percentItemType" abstract="false" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsForfeitureRate" />

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  <element name="ZertanePatentsMember" type="nonnum:domainItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_ZertanePatentsMember" />

  <element name="OrpMember" type="nonnum:domainItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_OrpMember" />

  <element name="FiniteLivedIntangibleAssetsExpirationMonthYear" type="xbrli:gYearMonthItemType" abstract="false" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_FiniteLivedIntangibleAssetsExpirationMonthYear" />

  <element name="PaidOnJuly12016Member" type="nonnum:domainItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_PaidOnJuly12016Member" />

  <element name="UnderwritersExercisedOfOverallotmentOption" type="xbrli:sharesItemType" abstract="false" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_UnderwritersExercisedOfOverallotmentOption" />

  <element name="PhoneSystemAndEnterpriseResourceSystem" type="xbrli:monetaryItemType" abstract="false" xbrli:periodType="instant" nillable="true" xbrli:balance="debit" substitutionGroup="xbrli:item" id="aytu_PhoneSystemAndEnterpriseResourceSystem" />

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  <element name="ExercisePriceRangeTwoMember" type="nonnum:domainItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_ExercisePriceRangeTwoMember" />

  <element name="ExercisePriceRangeThreeMember" type="nonnum:domainItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_ExercisePriceRangeThreeMember" />

  <element name="PlacementAgentWarrantsMember" type="nonnum:domainItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_PlacementAgentWarrantsMember" />

  <element name="InvestorsWarrantsMember" type="nonnum:domainItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_InvestorsWarrantsMember" />

  <element name="ConvertiblePromissoryNotesMay2016Member" type="nonnum:domainItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_ConvertiblePromissoryNotesMay2016Member" />

  <element name="ScheduleOfSharebasedPaymentAwardWarrantsValuationAssumptionsTableTextBlock" type="nonnum:textBlockItemType" abstract="false" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_ScheduleOfSharebasedPaymentAwardWarrantsValuationAssumptionsTableTextBlock" />

  <element name="CustomerOneMember" type="nonnum:domainItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_CustomerOneMember" />

  <element name="SalesRevenueGrossMember" type="nonnum:domainItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_SalesRevenueGrossMember" />

  <element name="AccountsReceivableNetMember" type="nonnum:domainItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_AccountsReceivableNetMember" />

  <element name="SummaryOfSignificantAccountingPolicyLineItems" type="xbrli:stringItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_SummaryOfSignificantAccountingPolicyLineItems" />

  <element name="StockOptionsMember" type="nonnum:domainItemType" abstract="true" xbrli:periodType="duration" nillable="true" substitutionGroup="xbrli:item" id="aytu_StockOptionsMember" />
</schema>

