    <SEC-DOCUMENT>0001144204-16-129428-index.html : 20161025
    <SEC-HEADER>0001144204-16-129428.hdr.sgml : 20161025
    <ACCEPTANCE-DATETIME>20161025171457
    ACCESSION NUMBER:		0001144204-16-129428
    CONFORMED SUBMISSION TYPE:	S-1/A
    PUBLIC DOCUMENT COUNT:		12
    FILED AS OF DATE:		20161025
    DATE AS OF CHANGE:		20161025

    FILER:

    	COMPANY DATA:
    		COMPANY CONFORMED NAME:			AYTU BIOSCIENCE, INC
    		CENTRAL INDEX KEY:			0001385818
    		STANDARD INDUSTRIAL CLASSIFICATION:	PHARMACEUTICAL PREPARATIONS [2834]
    		IRS NUMBER:				470883144
    		STATE OF INCORPORATION:			CO
    		FISCAL YEAR END:			0630

    	FILING VALUES:
    		FORM TYPE:		S-1/A
    		SEC ACT:		1933 Act
    		SEC FILE NUMBER:	333-213738
    		FILM NUMBER:		161951010

    	BUSINESS ADDRESS:
    		STREET 1:		373 INVERNESS PARKWAY
    		STREET 2:		SUITE 200
    		CITY:			ENGLEWOOD
    		STATE:			CO
    		ZIP:			80112
    		BUSINESS PHONE:		(720) 437-6580

    	MAIL ADDRESS:
    		STREET 1:		373 INVERNESS PARKWAY
    		STREET 2:		SUITE 200
    		CITY:			ENGLEWOOD
    		STATE:			CO
    		ZIP:			80112

    	FORMER COMPANY:
    		FORMER CONFORMED NAME:	AYTU BIOSCIENCE, INC.
    		DATE OF NAME CHANGE:	20150609

    	FORMER COMPANY:
    		FORMER CONFORMED NAME:	Rosewind CORP
    		DATE OF NAME CHANGE:	20070110
    </SEC-HEADER>
    <DOCUMENT>
    <TYPE>S-1/A
    <SEQUENCE>1
    <FILENAME>v450798_s1a.htm
    <DESCRIPTION>S-1/A
    <TEXT>
    Document 1 - file: v450798_s1a.htm

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>EX-4.6
    <SEQUENCE>2
    <FILENAME>v450798_ex4-6.htm
    <DESCRIPTION>EXHIBIT 4.6
    <TEXT>
    Document 2 - file: v450798_ex4-6.htm

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>EX-23.1
    <SEQUENCE>3
    <FILENAME>v450214_ex23x1.htm
    <DESCRIPTION>EXHIBIT 23.1
    <TEXT>
    Document 3 - file: v450214_ex23x1.htm

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>GRAPHIC
    <SEQUENCE>4
    <FILENAME>line.gif
    <DESCRIPTION>GRAPHIC
    <TEXT>
    Document 4 - file: line.gif

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    <DOCUMENT>
    <TYPE>GRAPHIC
    <SEQUENCE>5
    <FILENAME>spacer.gif
    <DESCRIPTION>GRAPHIC
    <TEXT>
    Document 5 - file: spacer.gif

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>GRAPHIC
    <SEQUENCE>6
    <FILENAME>logo_aytu-bioscience.jpg
    <DESCRIPTION>GRAPHIC
    <TEXT>
    Document 6 - file: logo_aytu-bioscience.jpg

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>GRAPHIC
    <SEQUENCE>7
    <FILENAME>v449009_ifc.jpg
    <DESCRIPTION>GRAPHIC
    <TEXT>
    Document 7 - file: v449009_ifc.jpg

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>GRAPHIC
    <SEQUENCE>8
    <FILENAME>v449009_chrt-org.jpg
    <DESCRIPTION>GRAPHIC
    <TEXT>
    Document 8 - file: v449009_chrt-org.jpg

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>GRAPHIC
    <SEQUENCE>9
    <FILENAME>v449009_img-testnasal.jpg
    <DESCRIPTION>GRAPHIC
    <TEXT>
    Document 9 - file: v449009_img-testnasal.jpg

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>GRAPHIC
    <SEQUENCE>10
    <FILENAME>v449009_chrt-line.jpg
    <DESCRIPTION>GRAPHIC
    <TEXT>
    Document 10 - file: v449009_chrt-line.jpg

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>GRAPHIC
    <SEQUENCE>11
    <FILENAME>v449009_img1.jpg
    <DESCRIPTION>GRAPHIC
    <TEXT>
    Document 11 - file: v449009_img1.jpg

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>GRAPHIC
    <SEQUENCE>12
    <FILENAME>v449009_img2.jpg
    <DESCRIPTION>GRAPHIC
    <TEXT>
    Document 12 - file: v449009_img2.jpg

    </DOCUMENT>
    </SEC-DOCUMENT>