    <SEC-DOCUMENT>0001213900-18-002353-index.html : 20180228
    <SEC-HEADER>0001213900-18-002353.hdr.sgml : 20180228
    <ACCEPTANCE-DATETIME>20180227214009
    ACCESSION NUMBER:		0001213900-18-002353
    CONFORMED SUBMISSION TYPE:	S-1/A
    PUBLIC DOCUMENT COUNT:		16
    FILED AS OF DATE:		20180228
    DATE AS OF CHANGE:		20180227

    FILER:

    	COMPANY DATA:
    		COMPANY CONFORMED NAME:			AYTU BIOSCIENCE, INC
    		CENTRAL INDEX KEY:			0001385818
    		STANDARD INDUSTRIAL CLASSIFICATION:	PHARMACEUTICAL PREPARATIONS [2834]
    		IRS NUMBER:				470883144
    		STATE OF INCORPORATION:			DE
    		FISCAL YEAR END:			0630

    	FILING VALUES:
    		FORM TYPE:		S-1/A
    		SEC ACT:		1933 Act
    		SEC FILE NUMBER:	333-222994
    		FILM NUMBER:		18647911

    	BUSINESS ADDRESS:
    		STREET 1:		373 INVERNESS PARKWAY
    		STREET 2:		SUITE 206
    		CITY:			ENGLEWOOD
    		STATE:			CO
    		ZIP:			80112
    		BUSINESS PHONE:		(720) 437-6580

    	MAIL ADDRESS:
    		STREET 1:		373 INVERNESS PARKWAY
    		STREET 2:		SUITE 206
    		CITY:			ENGLEWOOD
    		STATE:			CO
    		ZIP:			80112

    	FORMER COMPANY:
    		FORMER CONFORMED NAME:	AYTU BIOSCIENCE, INC.
    		DATE OF NAME CHANGE:	20150609

    	FORMER COMPANY:
    		FORMER CONFORMED NAME:	Rosewind CORP
    		DATE OF NAME CHANGE:	20070110
    </SEC-HEADER>
    <DOCUMENT>
    <TYPE>S-1/A
    <SEQUENCE>1
    <FILENAME>fs12018a4_aytubioscience.htm
    <DESCRIPTION>AMENDMENT NO.4 TO FORM S-1
    <TEXT>
    Document 1 - file: fs12018a4_aytubioscience.htm

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>EX-1.1
    <SEQUENCE>2
    <FILENAME>fs12018a4ex1-1_aytubio.htm
    <DESCRIPTION>FORM OF UNDERWRITING AGREEMENT BY AND BETWEEN THE COMPANY AND JOSEPH GUNNAR & CO., LLC AS REPRESENTATIVE OF THE SEVERAL UNDERWRITERS NAMED THEREIN
    <TEXT>
    Document 2 - file: fs12018a4ex1-1_aytubio.htm

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>EX-5.1
    <SEQUENCE>3
    <FILENAME>fs12018a4ex5-1_aytubio.htm
    <DESCRIPTION>OPINION OF SICHENZIA ROSS FERENCE KESNER LLP
    <TEXT>
    Document 3 - file: fs12018a4ex5-1_aytubio.htm

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>GRAPHIC
    <SEQUENCE>4
    <FILENAME>image_001.jpg
    <DESCRIPTION>GRAPHIC
    <TEXT>
    Document 4 - file: image_001.jpg

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>GRAPHIC
    <SEQUENCE>5
    <FILENAME>cover.jpg
    <DESCRIPTION>GRAPHIC
    <TEXT>
    Document 5 - file: cover.jpg

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>GRAPHIC
    <SEQUENCE>6
    <FILENAME>image_002.jpg
    <DESCRIPTION>GRAPHIC
    <TEXT>
    Document 6 - file: image_002.jpg

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>GRAPHIC
    <SEQUENCE>7
    <FILENAME>image_003.jpg
    <DESCRIPTION>GRAPHIC
    <TEXT>
    Document 7 - file: image_003.jpg

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>GRAPHIC
    <SEQUENCE>8
    <FILENAME>image_004.jpg
    <DESCRIPTION>GRAPHIC
    <TEXT>
    Document 8 - file: image_004.jpg

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>GRAPHIC
    <SEQUENCE>9
    <FILENAME>image_005.jpg
    <DESCRIPTION>GRAPHIC
    <TEXT>
    Document 9 - file: image_005.jpg

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>GRAPHIC
    <SEQUENCE>10
    <FILENAME>image_006.jpg
    <DESCRIPTION>GRAPHIC
    <TEXT>
    Document 10 - file: image_006.jpg

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>GRAPHIC
    <SEQUENCE>11
    <FILENAME>image_007.jpg
    <DESCRIPTION>GRAPHIC
    <TEXT>
    Document 11 - file: image_007.jpg

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>GRAPHIC
    <SEQUENCE>12
    <FILENAME>image_008.jpg
    <DESCRIPTION>GRAPHIC
    <TEXT>
    Document 12 - file: image_008.jpg

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>GRAPHIC
    <SEQUENCE>13
    <FILENAME>image_009.jpg
    <DESCRIPTION>GRAPHIC
    <TEXT>
    Document 13 - file: image_009.jpg

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>GRAPHIC
    <SEQUENCE>14
    <FILENAME>image_010.jpg
    <DESCRIPTION>GRAPHIC
    <TEXT>
    Document 14 - file: image_010.jpg

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>GRAPHIC
    <SEQUENCE>15
    <FILENAME>image_011.jpg
    <DESCRIPTION>GRAPHIC
    <TEXT>
    Document 15 - file: image_011.jpg

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>GRAPHIC
    <SEQUENCE>16
    <FILENAME>image_012.jpg
    <DESCRIPTION>GRAPHIC
    <TEXT>
    Document 16 - file: image_012.jpg

    </DOCUMENT>
    </SEC-DOCUMENT>