    <SEC-DOCUMENT>0001213900-21-007759-index.html : 20210209
    <SEC-HEADER>0001213900-21-007759.hdr.sgml : 20210209
    <ACCEPTANCE-DATETIME>20210209163951
    ACCESSION NUMBER:		0001213900-21-007759
    CONFORMED SUBMISSION TYPE:	424B3
    PUBLIC DOCUMENT COUNT:		5
    FILED AS OF DATE:		20210209
    DATE AS OF CHANGE:		20210209

    FILER:

    	COMPANY DATA:
    		COMPANY CONFORMED NAME:			AYTU BIOSCIENCE, INC
    		CENTRAL INDEX KEY:			0001385818
    		STANDARD INDUSTRIAL CLASSIFICATION:	PHARMACEUTICAL PREPARATIONS [2834]
    		IRS NUMBER:				470883144
    		STATE OF INCORPORATION:			DE
    		FISCAL YEAR END:			0630

    	FILING VALUES:
    		FORM TYPE:		424B3
    		SEC ACT:		1933 Act
    		SEC FILE NUMBER:	333-252450
    		FILM NUMBER:		21607088

    	BUSINESS ADDRESS:
    		STREET 1:		373 INVERNESS PARKWAY
    		STREET 2:		SUITE 206
    		CITY:			ENGLEWOOD
    		STATE:			CO
    		ZIP:			80112
    		BUSINESS PHONE:		(720) 437-6580

    	MAIL ADDRESS:
    		STREET 1:		373 INVERNESS PARKWAY
    		STREET 2:		SUITE 206
    		CITY:			ENGLEWOOD
    		STATE:			CO
    		ZIP:			80112

    	FORMER COMPANY:
    		FORMER CONFORMED NAME:	AYTU BIOSCIENCE, INC.
    		DATE OF NAME CHANGE:	20150609

    	FORMER COMPANY:
    		FORMER CONFORMED NAME:	Rosewind CORP
    		DATE OF NAME CHANGE:	20070110
    </SEC-HEADER>
    <DOCUMENT>
    <TYPE>424B3
    <SEQUENCE>1
    <FILENAME>ea135024-424b3_aytubio.htm
    <DESCRIPTION>PROSPECTUS SUPPLEMENT
    <TEXT>
    Document 1 - file: ea135024-424b3_aytubio.htm

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>GRAPHIC
    <SEQUENCE>2
    <FILENAME>img_001.jpg
    <DESCRIPTION>GRAPHIC
    <TEXT>
    Document 2 - file: img_001.jpg

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>GRAPHIC
    <SEQUENCE>3
    <FILENAME>img_002.jpg
    <DESCRIPTION>GRAPHIC
    <TEXT>
    Document 3 - file: img_002.jpg

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>GRAPHIC
    <SEQUENCE>4
    <FILENAME>img_003.jpg
    <DESCRIPTION>GRAPHIC
    <TEXT>
    Document 4 - file: img_003.jpg

    </DOCUMENT>
    <DOCUMENT>
    <TYPE>GRAPHIC
    <SEQUENCE>5
    <FILENAME>img_004.jpg
    <DESCRIPTION>GRAPHIC
    <TEXT>
    Document 5 - file: img_004.jpg

    </DOCUMENT>
    </SEC-DOCUMENT>