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Note 9 - Income Taxes - Deferred Tax Assets and Liabilities (Details) - USD ($) | Dec. 31, 2021 | Dec. 31, 2020
--- | --- | ---
Net operating loss carryforward | $ 18,449,694 | $ 14,737,240
Research and development tax credit carryforward | 1,566,293 | 1,189,110
Stock-based compensation expense | 129,475 | 4,870
Accrued salaries | 69,940 | 72,721
Total deferred tax assets | 20,215,402 | 16,003,941
Depreciation | 30,945 | 28,274
Net deferred tax assets | 20,184,457 | 15,975,667
Valuation allowance | (20,184,457) | (15,975,667)
Net deferred tax asset after reduction for valuation allowance | $ 0 | $ 0

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- Definition Amount, before allocation of valuation allowances and after deferred tax liability, of deferred tax asset attributable to deductible differences and carryforwards, without jurisdictional netting. + References No definition available. + Details
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- Definition Amount, after allocation of valuation allowances and deferred tax liability, of deferred tax asset attributable to deductible differences and carryforwards, without jurisdictional netting. + References Reference 1: http://www.xbrl.org/2009/role/commonPracticeRef -Publisher FASB -Name Accounting Standards Codification -Topic 740 -SubTopic 10 -Section 50 -Paragraph 2 -URI http://asc.fasb.org/extlink&oid=121826272&loc=d3e32537-109319 + Details
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