<SEC-DOCUMENT>0001213900-25-002157.txt : 20250108
<SEC-HEADER>0001213900-25-002157.hdr.sgml : 20250108
<ACCEPTANCE-DATETIME>20250108174830
ACCESSION NUMBER:		0001213900-25-002157
CONFORMED SUBMISSION TYPE:	4
PUBLIC DOCUMENT COUNT:		1
CONFORMED PERIOD OF REPORT:	20250107
FILED AS OF DATE:		20250108
DATE AS OF CHANGE:		20250108

REPORTING-OWNER:	

	OWNER DATA:	
		COMPANY CONFORMED NAME:			Parmer George
		CENTRAL INDEX KEY:			0001626673
		ORGANIZATION NAME:           	

	FILING VALUES:
		FORM TYPE:		4
		SEC ACT:		1934 Act
		SEC FILE NUMBER:	001-39553
		FILM NUMBER:		25519505

	MAIL ADDRESS:	
		STREET 1:		102 WEST BAKER STREET
		CITY:			PLANT CITY
		STATE:			FL
		ZIP:			33563

ISSUER:		

	COMPANY DATA:	
		COMPANY CONFORMED NAME:			Amesite Inc.
		CENTRAL INDEX KEY:			0001807166
		STANDARD INDUSTRIAL CLASSIFICATION:	SERVICES-PREPACKAGED SOFTWARE [7372]
		ORGANIZATION NAME:           	06 Technology
		IRS NUMBER:				823431717
		STATE OF INCORPORATION:			DE
		FISCAL YEAR END:			0630

	BUSINESS ADDRESS:	
		STREET 1:		205 EAST WASHINGTON STREET
		STREET 2:		SUITE B
		CITY:			ANN ARBOR
		STATE:			MI
		ZIP:			48104
		BUSINESS PHONE:		(650) 516-7633

	MAIL ADDRESS:	
		STREET 1:		205 EAST WASHINGTON STREET
		STREET 2:		SUITE B
		CITY:			ANN ARBOR
		STATE:			MI
		ZIP:			48104

	FORMER COMPANY:	
		FORMER CONFORMED NAME:	Amesite Operating Co
		DATE OF NAME CHANGE:	20200318
</SEC-HEADER>
<DOCUMENT>
<TYPE>4
<SEQUENCE>1
<FILENAME>ownership.xml
<TEXT>
<XML>
<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0508</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2025-01-07</periodOfReport>

    <notSubjectToSection16>0</notSubjectToSection16>

    <issuer>
        <issuerCik>0001807166</issuerCik>
        <issuerName>Amesite Inc.</issuerName>
        <issuerTradingSymbol>AMST</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001626673</rptOwnerCik>
            <rptOwnerName>Parmer George</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O AMESITE INC.</rptOwnerStreet1>
            <rptOwnerStreet2>607 SHELBY STREET, SUITE 700 PMB 214</rptOwnerStreet2>
            <rptOwnerCity>DETROIT</rptOwnerCity>
            <rptOwnerState>MI</rptOwnerState>
            <rptOwnerZipCode>48226</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>1</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>0</isTenPercentOwner>
            <isOther>0</isOther>
            <officerTitle></officerTitle>
            <otherText></otherText>
        </reportingOwnerRelationship>
    </reportingOwner>

    <aff10b5One>0</aff10b5One>

    <nonDerivativeTable>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Common Stock</value>
            </securityTitle>
            <transactionDate>
                <value>2025-01-07</value>
            </transactionDate>
            <deemedExecutionDate></deemedExecutionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>P</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
            </transactionCoding>
            <transactionTimeliness></transactionTimeliness>
            <transactionAmounts>
                <transactionShares>
                    <value>83333</value>
                </transactionShares>
                <transactionPricePerShare>
                    <value>3.00</value>
                    <footnoteId id="F1"/>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>A</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>189166</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">The shares were purchased in a confidentially marketed public offering pursuant to an underwriting agreement. For more information relating to the offering pursuant to which the reported shares were purchased, please refer to the final prospectus filed by Amesite Inc. with the SEC on January 7, 2025.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ Ann Marie Sastry Ph.D., as Attorney-in-Fact for George Parmer</signatureName>
        <signatureDate>2025-01-08</signatureDate>
    </ownerSignature>
</ownershipDocument>
</XML>
</TEXT>
</DOCUMENT>
</SEC-DOCUMENT>
