S-1 EX-FILING FEES 0001859807 0001859807 1 2025-08-28 2025-08-28 0001859807 2025-08-28 2025-08-28 iso4217:USD xbrli:pure xbrli:shares

Ex-Filing Fees

CALCULATION OF FILING FEE TABLES

S-1

Profusa, Inc.

Table 1: Newly Registered and Carry Forward Securities

Line Item Type |  | Security Type |  | Security Class Title |  | Notes |  | Fee Calculation Rule |  | Amount Registered |  | Proposed Maximum Offering Price Per Unit |  | Maximum Aggregate Offering Price |  | Fee Rate |  | Amount of Registration Fee
Newly Registered Securities
Fees to be Paid |  | Equity |  | Common Stock, $0.0001 par value per share |  | (1) |  | Other |  | 222,222,222 |  | $ | 0.41 |  | $ | 91,111,111.02 |  | 0.0001531 |  | $ | 13,949.11
Total Offering Amounts: |  | $ | 91,111,111.02 |  |  |  |  | 13,949.11
Total Fees Previously Paid: |  |  |  |  |  |  |  | 0.00
Total Fee Offsets: |  |  |  |  |  |  |  | 0.00
Net Fee Due: |  |  |  |  |  |  | $ | 13,949.11

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Offering Note(s)

(1) |  | Estimated solely for the purpose of calculating the registration fee in accordance with Rule 457(c) of the Securities Act of 1933, as amended, based upon the average of the high and low prices for a share of the registrant’s common stock as reported on The NASDAQ Stock Market on August 28, 2025. Pursuant to Rule 416 under the Securities Act, the securities being registered hereunder for the selling shareholders include such indeterminate number of additional shares of common stock as may be issued after the date hereof as a result of stock splits, stock dividends or similar transactions.