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STATEMENTS OF STOCKHOLDERS' EQUITY (DEFICIT) - USD ($) | Common Stock | Additional Paid-In Capital | Accumulated Deficit | Total
--- | --- | --- | --- | ---
Balance, beginning of period at Dec. 31, 2023 |  |  | $ 48,057,158 | $ (46,416,344) | $ 1,640,814 |
Balance, beginning of period (in shares) at Dec. 31, 2023 | [1] | 679 |  |  |  |
Increase (Decrease) in Stockholders' Equity (Deficit) |  |  |  |  |  |
Net loss |  |  |  | (3,326,812) | (3,326,812) |
Issuances of common stock, net |  | $ 1 | 6,087,140 |  | 6,087,141 |
Issuances of common stock, net (in shares) | [1] | 5,607 |  |  |  |
Share-based compensation |  |  | 183,152 |  | 183,152 |
Balance, end of period at Mar. 31, 2024 |  | $ 1 | 54,327,450 | (49,743,156) | 4,584,295 |
Balance, end of period (in shares) at Mar. 31, 2024 | [1] | 6,286 |  |  |  |
Balance, beginning of period at Dec. 31, 2023 |  |  | 48,057,158 | (46,416,344) | 1,640,814 |
Balance, beginning of period (in shares) at Dec. 31, 2023 | [1] | 679 |  |  |  |
Increase (Decrease) in Stockholders' Equity (Deficit) |  |  |  |  |  |
Net loss | [1] |  |  |  | (8,517,626) |
Balance, end of period at Jun. 30, 2024 |  | $ 1 | 56,256,243 | (54,933,970) | 1,322,274 |
Balance, end of period (in shares) at Jun. 30, 2024 | [1] | 7,863 |  |  |  |
Balance, beginning of period at Mar. 31, 2024 |  | $ 1 | 54,327,450 | (49,743,156) | 4,584,295 |
Balance, beginning of period (in shares) at Mar. 31, 2024 | [1] | 6,286 |  |  |  |
Increase (Decrease) in Stockholders' Equity (Deficit) |  |  |  |  |  |
Net loss |  |  |  | (5,190,814) | (5,190,814) | [1]
Issuances of common stock, net |  |  | 785,198 |  | 785,198 |
Issuances of common stock, net (in shares) | [1] | 1,577 |  |  |  |
Share-based compensation |  |  | 1,143,595 |  | 1,143,595 |
Balance, end of period at Jun. 30, 2024 |  | $ 1 | 56,256,243 | (54,933,970) | 1,322,274 |
Balance, end of period (in shares) at Jun. 30, 2024 | [1] | 7,863 |  |  |  |
Balance, beginning of period at Dec. 31, 2024 |  | $ 4 | 61,183,178 | (63,201,916) | (2,018,734) |
Balance, beginning of period (in shares) at Dec. 31, 2024 | [1] | 36,753 |  |  |  |
Increase (Decrease) in Stockholders' Equity (Deficit) |  |  |  |  |  |
Net loss |  |  |  | (12,085,413) | (12,085,413) |
Issuances of common stock, net |  | $ 80 | 8,854,717 |  | 8,854,797 |
Issuances of common stock, net (in shares) | [1] | 796,930 |  |  |  |
Exercise of Series D warrants |  |  | 15,384,319 |  | 15,384,319 |
Share-based compensation |  |  | 157,729 |  | 157,729 |
Balance, end of period at Mar. 31, 2025 |  | $ 84 | 85,579,943 | (75,287,329) | 10,292,698 |
Balance, end of period (in shares) at Mar. 31, 2025 | [1] | 833,683 |  |  |  |
Balance, beginning of period at Dec. 31, 2024 |  | $ 4 | 61,183,178 | (63,201,916) | (2,018,734) |
Balance, beginning of period (in shares) at Dec. 31, 2024 | [1] | 36,753 |  |  |  |
Increase (Decrease) in Stockholders' Equity (Deficit) |  |  |  |  |  |
Net loss | [1] |  |  |  | (16,361,915) |
Balance, end of period at Jun. 30, 2025 |  | $ 84 | 85,711,453 | (79,563,831) | 6,147,706 |
Balance, end of period (in shares) at Jun. 30, 2025 | [1] | 833,683 |  |  |  |
Balance, beginning of period at Mar. 31, 2025 |  | $ 84 | 85,579,943 | (75,287,329) | 10,292,698 |
Balance, beginning of period (in shares) at Mar. 31, 2025 | [1] | 833,683 |  |  |  |
Increase (Decrease) in Stockholders' Equity (Deficit) |  |  |  |  |  |
Net loss |  |  |  | (4,276,502) | (4,276,502) | [1]
Share-based compensation |  |  | 131,510 |  | 131,510 |
Balance, end of period at Jun. 30, 2025 |  | $ 84 | $ 85,711,453 | $ (79,563,831) | $ 6,147,706 |
Balance, end of period (in shares) at Jun. 30, 2025 | [1] | 833,683 |  |  |  |
[1] | On May 15, 2025, the Company effected a reverse split of the Company's common stock, either issued and outstanding or held by the Company as treasury stock. The May 2025 Reverse Split was at a ratio of one share for every 28 shares previously held with no change in the par value per share. The May 2025 Reverse Split did not change the number of authorized shares of common stock. All common stock share and per share data, and exercise price data for applicable common stock equivalents, included in these financial statements have been retroactively adjusted to reflect the Reverse Split.

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