<?xml version="1.0" encoding="US-ASCII"?>
<!--XBRL Document Created with Wdesk from Workiva-->
<!-- -->
<link:linkbase xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd" xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/BusinessRiskAndCreditRiskConcentrationInvolvingCash" xlink:href="astc-20170930.xsd#BusinessRiskAndCreditRiskConcentrationInvolvingCash" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/BusinessRiskAndCreditRiskConcentrationInvolvingCashDetails" xlink:href="astc-20170930.xsd#BusinessRiskAndCreditRiskConcentrationInvolvingCashDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/BusinessRiskAndCreditRiskConcentrationInvolvingCashTables" xlink:href="astc-20170930.xsd#BusinessRiskAndCreditRiskConcentrationInvolvingCashTables" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/CommitmentsAndContingencies" xlink:href="astc-20170930.xsd#CommitmentsAndContingencies" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/CommonStockIncentiveStockPurchasePlansAndOtherCompensationPlans" xlink:href="astc-20170930.xsd#CommonStockIncentiveStockPurchasePlansAndOtherCompensationPlans" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/CommonStockIncentiveStockPurchasePlansAndOtherCompensationPlansNarrativeDetails" xlink:href="astc-20170930.xsd#CommonStockIncentiveStockPurchasePlansAndOtherCompensationPlansNarrativeDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/CommonStockIncentiveStockPurchasePlansAndOtherCompensationPlansRestrictedStockDetails" xlink:href="astc-20170930.xsd#CommonStockIncentiveStockPurchasePlansAndOtherCompensationPlansRestrictedStockDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/CommonStockIncentiveStockPurchasePlansAndOtherCompensationPlansStockOptionActivityDetails" xlink:href="astc-20170930.xsd#CommonStockIncentiveStockPurchasePlansAndOtherCompensationPlansStockOptionActivityDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/CommonStockIncentiveStockPurchasePlansAndOtherCompensationPlansStockOptionsByExercisePriceDetails" xlink:href="astc-20170930.xsd#CommonStockIncentiveStockPurchasePlansAndOtherCompensationPlansStockOptionsByExercisePriceDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/CommonStockIncentiveStockPurchasePlansAndOtherCompensationPlansTables" xlink:href="astc-20170930.xsd#CommonStockIncentiveStockPurchasePlansAndOtherCompensationPlansTables" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/CondensedConsolidatedBalanceSheets" xlink:href="astc-20170930.xsd#CondensedConsolidatedBalanceSheets" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/CondensedConsolidatedBalanceSheetsParenthetical" xlink:href="astc-20170930.xsd#CondensedConsolidatedBalanceSheetsParenthetical" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/CondensedConsolidatedStatementsOfCashFlows" xlink:href="astc-20170930.xsd#CondensedConsolidatedStatementsOfCashFlows" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/CondensedConsolidatedStatementsOfOperationsAndComprehensiveLoss" xlink:href="astc-20170930.xsd#CondensedConsolidatedStatementsOfOperationsAndComprehensiveLoss" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/CondensedConsolidatedStatementsOfOperationsAndComprehensiveLossCalc2" xlink:href="astc-20170930.xsd#CondensedConsolidatedStatementsOfOperationsAndComprehensiveLossCalc2" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/DocumentAndEntityInformation" xlink:href="astc-20170930.xsd#DocumentAndEntityInformation" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/FairValueMeasurement" xlink:href="astc-20170930.xsd#FairValueMeasurement" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/FairValueMeasurementDetails" xlink:href="astc-20170930.xsd#FairValueMeasurementDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/FairValueMeasurementTables" xlink:href="astc-20170930.xsd#FairValueMeasurementTables" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/GeneralInformation" xlink:href="astc-20170930.xsd#GeneralInformation" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/GeneralInformationDetails" xlink:href="astc-20170930.xsd#GeneralInformationDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/GeneralInformationPolicies" xlink:href="astc-20170930.xsd#GeneralInformationPolicies" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/GoingConcernDetails" xlink:href="astc-20170930.xsd#GoingConcernDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/GoingConcernGoingConcern" xlink:href="astc-20170930.xsd#GoingConcernGoingConcern" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/IncomeTaxes" xlink:href="astc-20170930.xsd#IncomeTaxes" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/IncomeTaxesDetails" xlink:href="astc-20170930.xsd#IncomeTaxesDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/Inventory" xlink:href="astc-20170930.xsd#Inventory" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/InventoryDetails" xlink:href="astc-20170930.xsd#InventoryDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/InventoryTables" xlink:href="astc-20170930.xsd#InventoryTables" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/Investments" xlink:href="astc-20170930.xsd#Investments" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/InvestmentsAvailableForSaleSecuritiesDetails" xlink:href="astc-20170930.xsd#InvestmentsAvailableForSaleSecuritiesDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/InvestmentsCarryingValueDetails" xlink:href="astc-20170930.xsd#InvestmentsCarryingValueDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/InvestmentsTables" xlink:href="astc-20170930.xsd#InvestmentsTables" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/NetLossPerShare" xlink:href="astc-20170930.xsd#NetLossPerShare" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/NetLossPerShareBasicAndDilutedComputationDetails" xlink:href="astc-20170930.xsd#NetLossPerShareBasicAndDilutedComputationDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/NetLossPerShareNarrativeDetails" xlink:href="astc-20170930.xsd#NetLossPerShareNarrativeDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/NetLossPerShareTables" xlink:href="astc-20170930.xsd#NetLossPerShareTables" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/NoncontrollingInterest" xlink:href="astc-20170930.xsd#NoncontrollingInterest" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/NoncontrollingInterestChangesInEquityDetails" xlink:href="astc-20170930.xsd#NoncontrollingInterestChangesInEquityDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/NoncontrollingInterestContributionsDetails" xlink:href="astc-20170930.xsd#NoncontrollingInterestContributionsDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/NoncontrollingInterestNarrativeDetails" xlink:href="astc-20170930.xsd#NoncontrollingInterestNarrativeDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/NoncontrollingInterestTables" xlink:href="astc-20170930.xsd#NoncontrollingInterestTables" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/OtherComprehensiveLoss" xlink:href="astc-20170930.xsd#OtherComprehensiveLoss" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/OtherComprehensiveLossDetails" xlink:href="astc-20170930.xsd#OtherComprehensiveLossDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/OtherComprehensiveLossTables" xlink:href="astc-20170930.xsd#OtherComprehensiveLossTables" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/RevenueRecognition" xlink:href="astc-20170930.xsd#RevenueRecognition" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/RevenueRecognitionDetails" xlink:href="astc-20170930.xsd#RevenueRecognitionDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/SegmentInformation" xlink:href="astc-20170930.xsd#SegmentInformation" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/SegmentInformationNarrativeDetails" xlink:href="astc-20170930.xsd#SegmentInformationNarrativeDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/SegmentInformationScheduleOfSegmentsDetails" xlink:href="astc-20170930.xsd#SegmentInformationScheduleOfSegmentsDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/SegmentInformationTables" xlink:href="astc-20170930.xsd#SegmentInformationTables" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/SubsequentEvents" xlink:href="astc-20170930.xsd#SubsequentEvents" xlink:type="simple" />
  <link:roleRef roleURI="http://www.astrotechcorp.com/role/SubsequentEventsDetails" xlink:href="astc-20170930.xsd#SubsequentEventsDetails" xlink:type="simple" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/BusinessRiskAndCreditRiskConcentrationInvolvingCash" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/BusinessRiskAndCreditRiskConcentrationInvolvingCashDetails" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/BusinessRiskAndCreditRiskConcentrationInvolvingCashTables" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/CommitmentsAndContingencies" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/CommonStockIncentiveStockPurchasePlansAndOtherCompensationPlans" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/CommonStockIncentiveStockPurchasePlansAndOtherCompensationPlansNarrativeDetails" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/CommonStockIncentiveStockPurchasePlansAndOtherCompensationPlansRestrictedStockDetails" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/CommonStockIncentiveStockPurchasePlansAndOtherCompensationPlansStockOptionActivityDetails" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/CommonStockIncentiveStockPurchasePlansAndOtherCompensationPlansStockOptionsByExercisePriceDetails" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/CommonStockIncentiveStockPurchasePlansAndOtherCompensationPlansTables" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/CondensedConsolidatedBalanceSheets" xlink:type="extended">
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_LiabilitiesAndStockholdersEquity" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquity_159dcb00-b17e-17db-7098-be4159799459" xlink:type="locator" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_Liabilities" xlink:label="loc_us-gaap_Liabilities_d8bb821f-d155-9d4e-1738-736b4bf4cb19" xlink:type="locator" />
    <link:calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_159dcb00-b17e-17db-7098-be4159799459" xlink:to="loc_us-gaap_Liabilities_d8bb821f-d155-9d4e-1738-736b4bf4cb19" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_LiabilitiesCurrent" xlink:label="loc_us-gaap_LiabilitiesCurrent_f5e246cf-e80f-d00b-c8ed-4796aa2df4b4" xlink:type="locator" />
    <link:calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_d8bb821f-d155-9d4e-1738-736b4bf4cb19" xlink:to="loc_us-gaap_LiabilitiesCurrent_f5e246cf-e80f-d00b-c8ed-4796aa2df4b4" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_AccountsPayableCurrent" xlink:label="loc_us-gaap_AccountsPayableCurrent_7a72b51a-735c-1ad4-bd3f-d9bcf2d6a26d" xlink:type="locator" />
    <link:calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_f5e246cf-e80f-d00b-c8ed-4796aa2df4b4" xlink:to="loc_us-gaap_AccountsPayableCurrent_7a72b51a-735c-1ad4-bd3f-d9bcf2d6a26d" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_EmployeeRelatedLiabilitiesCurrent" xlink:label="loc_us-gaap_EmployeeRelatedLiabilitiesCurrent_cf9ed09f-3dd1-4a4b-d3fc-3b0db18c1b40" xlink:type="locator" />
    <link:calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_f5e246cf-e80f-d00b-c8ed-4796aa2df4b4" xlink:to="loc_us-gaap_EmployeeRelatedLiabilitiesCurrent_cf9ed09f-3dd1-4a4b-d3fc-3b0db18c1b40" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_AccruedLiabilitiesCurrent" xlink:label="loc_us-gaap_AccruedLiabilitiesCurrent_3fe8f96b-142b-97b1-3b39-b8d22cd10e98" xlink:type="locator" />
    <link:calculationArc order="3" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_f5e246cf-e80f-d00b-c8ed-4796aa2df4b4" xlink:to="loc_us-gaap_AccruedLiabilitiesCurrent_3fe8f96b-142b-97b1-3b39-b8d22cd10e98" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_TaxesPayableCurrent" xlink:label="loc_us-gaap_TaxesPayableCurrent_8544ed0a-bea0-b1fa-ce65-66a1927d2672" xlink:type="locator" />
    <link:calculationArc order="4" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_f5e246cf-e80f-d00b-c8ed-4796aa2df4b4" xlink:to="loc_us-gaap_TaxesPayableCurrent_8544ed0a-bea0-b1fa-ce65-66a1927d2672" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_OtherLiabilitiesNoncurrent" xlink:label="loc_us-gaap_OtherLiabilitiesNoncurrent_f234998c-5e05-09d0-d53e-5c44423c97c0" xlink:type="locator" />
    <link:calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_d8bb821f-d155-9d4e-1738-736b4bf4cb19" xlink:to="loc_us-gaap_OtherLiabilitiesNoncurrent_f234998c-5e05-09d0-d53e-5c44423c97c0" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_CommitmentsAndContingencies" xlink:label="loc_us-gaap_CommitmentsAndContingencies_058e01e4-b9a1-623e-29f5-1b5c79bf095a" xlink:type="locator" />
    <link:calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_159dcb00-b17e-17db-7098-be4159799459" xlink:to="loc_us-gaap_CommitmentsAndContingencies_058e01e4-b9a1-623e-29f5-1b5c79bf095a" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_StockholdersEquity" xlink:label="loc_us-gaap_StockholdersEquity_4b158f76-5f04-5e4d-ead3-d80e1f9943db" xlink:type="locator" />
    <link:calculationArc order="3" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_159dcb00-b17e-17db-7098-be4159799459" xlink:to="loc_us-gaap_StockholdersEquity_4b158f76-5f04-5e4d-ead3-d80e1f9943db" xlink:type="arc" />
    <link:calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_4b158f76-5f04-5e4d-ead3-d80e1f9943db" xlink:to="loc_us-gaap_CommitmentsAndContingencies_058e01e4-b9a1-623e-29f5-1b5c79bf095a" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_PreferredStockValue" xlink:label="loc_us-gaap_PreferredStockValue_4fb6195d-d905-dac7-e5a0-79f324993f79" xlink:type="locator" />
    <link:calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_4b158f76-5f04-5e4d-ead3-d80e1f9943db" xlink:to="loc_us-gaap_PreferredStockValue_4fb6195d-d905-dac7-e5a0-79f324993f79" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_CommonStockValue" xlink:label="loc_us-gaap_CommonStockValue_c3f7606a-e7e9-61d5-b60b-b4b059f68b26" xlink:type="locator" />
    <link:calculationArc order="3" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_4b158f76-5f04-5e4d-ead3-d80e1f9943db" xlink:to="loc_us-gaap_CommonStockValue_c3f7606a-e7e9-61d5-b60b-b4b059f68b26" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_TreasuryStockValue" xlink:label="loc_us-gaap_TreasuryStockValue_40678492-c421-af67-b276-f663f9ff2f98" xlink:type="locator" />
    <link:calculationArc order="4" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_4b158f76-5f04-5e4d-ead3-d80e1f9943db" xlink:to="loc_us-gaap_TreasuryStockValue_40678492-c421-af67-b276-f663f9ff2f98" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_AdditionalPaidInCapital" xlink:label="loc_us-gaap_AdditionalPaidInCapital_2d1b9d27-8b19-82d9-2843-5b3d292b7fbb" xlink:type="locator" />
    <link:calculationArc order="5" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_4b158f76-5f04-5e4d-ead3-d80e1f9943db" xlink:to="loc_us-gaap_AdditionalPaidInCapital_2d1b9d27-8b19-82d9-2843-5b3d292b7fbb" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_RetainedEarningsAccumulatedDeficit" xlink:label="loc_us-gaap_RetainedEarningsAccumulatedDeficit_065cb284-7fca-cc9b-5752-2feaf74d0011" xlink:type="locator" />
    <link:calculationArc order="6" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_4b158f76-5f04-5e4d-ead3-d80e1f9943db" xlink:to="loc_us-gaap_RetainedEarningsAccumulatedDeficit_065cb284-7fca-cc9b-5752-2feaf74d0011" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_04f0961a-a0bc-88cf-6658-3f950da4a458" xlink:type="locator" />
    <link:calculationArc order="7" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_4b158f76-5f04-5e4d-ead3-d80e1f9943db" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_04f0961a-a0bc-88cf-6658-3f950da4a458" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_Assets" xlink:label="loc_us-gaap_Assets_c6057b1d-6685-8249-2051-6c57f3a16a9e" xlink:type="locator" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_AssetsCurrent" xlink:label="loc_us-gaap_AssetsCurrent_8a47066b-3628-88d1-49db-ed09aecc7fc7" xlink:type="locator" />
    <link:calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_c6057b1d-6685-8249-2051-6c57f3a16a9e" xlink:to="loc_us-gaap_AssetsCurrent_8a47066b-3628-88d1-49db-ed09aecc7fc7" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_07da1db4-2708-4446-4250-1fdd5956185e" xlink:type="locator" />
    <link:calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_8a47066b-3628-88d1-49db-ed09aecc7fc7" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_07da1db4-2708-4446-4250-1fdd5956185e" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_ShortTermInvestments" xlink:label="loc_us-gaap_ShortTermInvestments_d585eba8-c5c0-6186-d29a-3180d2b06f0a" xlink:type="locator" />
    <link:calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_8a47066b-3628-88d1-49db-ed09aecc7fc7" xlink:to="loc_us-gaap_ShortTermInvestments_d585eba8-c5c0-6186-d29a-3180d2b06f0a" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_AccountsReceivableNetCurrent" xlink:label="loc_us-gaap_AccountsReceivableNetCurrent_dd7775cd-f4ab-5607-9b31-1ee98f605818" xlink:type="locator" />
    <link:calculationArc order="3" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_8a47066b-3628-88d1-49db-ed09aecc7fc7" xlink:to="loc_us-gaap_AccountsReceivableNetCurrent_dd7775cd-f4ab-5607-9b31-1ee98f605818" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_InventoryNet" xlink:label="loc_us-gaap_InventoryNet_7eab44cf-1ea1-ca2a-420f-21f7b5343fc3" xlink:type="locator" />
    <link:calculationArc order="4" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_8a47066b-3628-88d1-49db-ed09aecc7fc7" xlink:to="loc_us-gaap_InventoryNet_7eab44cf-1ea1-ca2a-420f-21f7b5343fc3" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_PrepaidExpenseAndOtherAssetsCurrent" xlink:label="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_2294e6a2-7bab-43ed-d02c-bdf73b8fa561" xlink:type="locator" />
    <link:calculationArc order="5" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_8a47066b-3628-88d1-49db-ed09aecc7fc7" xlink:to="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_2294e6a2-7bab-43ed-d02c-bdf73b8fa561" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_PropertyPlantAndEquipmentNet" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_1944a381-5c4e-ce3c-9111-13ccd1c639db" xlink:type="locator" />
    <link:calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_c6057b1d-6685-8249-2051-6c57f3a16a9e" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_1944a381-5c4e-ce3c-9111-13ccd1c639db" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_LongTermInvestments" xlink:label="loc_us-gaap_LongTermInvestments_c62504ca-c588-7676-b66d-68002db39ddb" xlink:type="locator" />
    <link:calculationArc order="3" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_c6057b1d-6685-8249-2051-6c57f3a16a9e" xlink:to="loc_us-gaap_LongTermInvestments_c62504ca-c588-7676-b66d-68002db39ddb" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_OtherAssetsNoncurrent" xlink:label="loc_us-gaap_OtherAssetsNoncurrent_9b27fab9-d497-627b-4cfb-6b585220b978" xlink:type="locator" />
    <link:calculationArc order="4" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_c6057b1d-6685-8249-2051-6c57f3a16a9e" xlink:to="loc_us-gaap_OtherAssetsNoncurrent_9b27fab9-d497-627b-4cfb-6b585220b978" xlink:type="arc" />
  </link:calculationLink>
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/CondensedConsolidatedBalanceSheetsParenthetical" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/CondensedConsolidatedStatementsOfCashFlows" xlink:type="extended">
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_CashAndCashEquivalentsPeriodIncreaseDecrease" xlink:label="loc_us-gaap_CashAndCashEquivalentsPeriodIncreaseDecrease_dd2e9db6-10f9-d78a-ba84-b18268245a83" xlink:type="locator" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_444b1ac7-f47a-4ce3-0caa-537f7e4ab8c3" xlink:type="locator" />
    <link:calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashAndCashEquivalentsPeriodIncreaseDecrease_dd2e9db6-10f9-d78a-ba84-b18268245a83" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_444b1ac7-f47a-4ce3-0caa-537f7e4ab8c3" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_ProfitLoss" xlink:label="loc_us-gaap_ProfitLoss_adacf05e-7e15-bd60-3e53-87432885dc29" xlink:type="locator" />
    <link:calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_444b1ac7-f47a-4ce3-0caa-537f7e4ab8c3" xlink:to="loc_us-gaap_ProfitLoss_adacf05e-7e15-bd60-3e53-87432885dc29" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_ShareBasedCompensation" xlink:label="loc_us-gaap_ShareBasedCompensation_e3eecf87-b290-4567-8a59-5b0a8e27c85b" xlink:type="locator" />
    <link:calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_444b1ac7-f47a-4ce3-0caa-537f7e4ab8c3" xlink:to="loc_us-gaap_ShareBasedCompensation_e3eecf87-b290-4567-8a59-5b0a8e27c85b" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_AdjustmentForAmortization" xlink:label="loc_us-gaap_AdjustmentForAmortization_0b36ab30-8e3e-508d-4854-83e9460175f2" xlink:type="locator" />
    <link:calculationArc order="3" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_444b1ac7-f47a-4ce3-0caa-537f7e4ab8c3" xlink:to="loc_us-gaap_AdjustmentForAmortization_0b36ab30-8e3e-508d-4854-83e9460175f2" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_Depreciation" xlink:label="loc_us-gaap_Depreciation_b4463f01-8926-c348-9c78-89e2ab0ed145" xlink:type="locator" />
    <link:calculationArc order="4" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_444b1ac7-f47a-4ce3-0caa-537f7e4ab8c3" xlink:to="loc_us-gaap_Depreciation_b4463f01-8926-c348-9c78-89e2ab0ed145" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_AvailableforsaleSecuritiesGrossRealizedGainLossExcludingOtherThanTemporaryImpairments" xlink:label="loc_us-gaap_AvailableforsaleSecuritiesGrossRealizedGainLossExcludingOtherThanTemporaryImpairments_43786c37-cf54-5da7-9a1f-a4e795eb3c41" xlink:type="locator" />
    <link:calculationArc order="5" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_444b1ac7-f47a-4ce3-0caa-537f7e4ab8c3" xlink:to="loc_us-gaap_AvailableforsaleSecuritiesGrossRealizedGainLossExcludingOtherThanTemporaryImpairments_43786c37-cf54-5da7-9a1f-a4e795eb3c41" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_IncreaseDecreaseInAccountsReceivable" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsReceivable_dd4bfa5e-1979-c817-e8a9-371104c0d4f7" xlink:type="locator" />
    <link:calculationArc order="6" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_444b1ac7-f47a-4ce3-0caa-537f7e4ab8c3" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsReceivable_dd4bfa5e-1979-c817-e8a9-371104c0d4f7" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_IncreaseDecreaseInCostInExcessOfBillingOnUncompletedContract" xlink:label="loc_us-gaap_IncreaseDecreaseInCostInExcessOfBillingOnUncompletedContract_5163b6ce-a513-2771-f953-fe7adbf2e9c7" xlink:type="locator" />
    <link:calculationArc order="7" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_444b1ac7-f47a-4ce3-0caa-537f7e4ab8c3" xlink:to="loc_us-gaap_IncreaseDecreaseInCostInExcessOfBillingOnUncompletedContract_5163b6ce-a513-2771-f953-fe7adbf2e9c7" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_IncreaseDecreaseInAccountsPayable" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsPayable_a8b6fe35-4809-bddf-7ef6-3144a6c89165" xlink:type="locator" />
    <link:calculationArc order="8" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_444b1ac7-f47a-4ce3-0caa-537f7e4ab8c3" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsPayable_a8b6fe35-4809-bddf-7ef6-3144a6c89165" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_IncreaseDecreaseInOtherOperatingCapitalNet" xlink:label="loc_us-gaap_IncreaseDecreaseInOtherOperatingCapitalNet_962cfc51-230d-a221-126b-1d12af7a5921" xlink:type="locator" />
    <link:calculationArc order="9" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_444b1ac7-f47a-4ce3-0caa-537f7e4ab8c3" xlink:to="loc_us-gaap_IncreaseDecreaseInOtherOperatingCapitalNet_962cfc51-230d-a221-126b-1d12af7a5921" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_70319186-e7bd-7189-88d2-eba26fb93350" xlink:type="locator" />
    <link:calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashAndCashEquivalentsPeriodIncreaseDecrease_dd2e9db6-10f9-d78a-ba84-b18268245a83" xlink:to="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_70319186-e7bd-7189-88d2-eba26fb93350" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_ProceedsFromSaleOfAvailableForSaleSecurities" xlink:label="loc_us-gaap_ProceedsFromSaleOfAvailableForSaleSecurities_d3d89aab-5be8-f6a4-66e9-41f55e0839cd" xlink:type="locator" />
    <link:calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_70319186-e7bd-7189-88d2-eba26fb93350" xlink:to="loc_us-gaap_ProceedsFromSaleOfAvailableForSaleSecurities_d3d89aab-5be8-f6a4-66e9-41f55e0839cd" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_ProceedsFromMaturitiesPrepaymentsAndCallsOfAvailableForSaleSecurities" xlink:label="loc_us-gaap_ProceedsFromMaturitiesPrepaymentsAndCallsOfAvailableForSaleSecurities_b49c7a2f-2986-824d-e7cb-bc082ac0e888" xlink:type="locator" />
    <link:calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_70319186-e7bd-7189-88d2-eba26fb93350" xlink:to="loc_us-gaap_ProceedsFromMaturitiesPrepaymentsAndCallsOfAvailableForSaleSecurities_b49c7a2f-2986-824d-e7cb-bc082ac0e888" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_PaymentsToAcquirePropertyPlantAndEquipment" xlink:label="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_fee12b15-6b42-6245-2e2f-ff030b98332c" xlink:type="locator" />
    <link:calculationArc order="3" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_70319186-e7bd-7189-88d2-eba26fb93350" xlink:to="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_fee12b15-6b42-6245-2e2f-ff030b98332c" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_deb49b68-c030-7624-a39d-ea301caa5dbf" xlink:type="locator" />
    <link:calculationArc order="3" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashAndCashEquivalentsPeriodIncreaseDecrease_dd2e9db6-10f9-d78a-ba84-b18268245a83" xlink:to="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_deb49b68-c030-7624-a39d-ea301caa5dbf" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_PaymentsForRepurchaseOfEquity" xlink:label="loc_us-gaap_PaymentsForRepurchaseOfEquity_fdeaa80c-12df-60f7-e738-4ae95c9ba521" xlink:type="locator" />
    <link:calculationArc order="1" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_deb49b68-c030-7624-a39d-ea301caa5dbf" xlink:to="loc_us-gaap_PaymentsForRepurchaseOfEquity_fdeaa80c-12df-60f7-e738-4ae95c9ba521" xlink:type="arc" />
  </link:calculationLink>
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/CondensedConsolidatedStatementsOfOperationsAndComprehensiveLoss" xlink:type="extended">
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_a53f52a4-7a54-3283-f734-95683c76ebff" xlink:type="locator" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_OperatingIncomeLoss" xlink:label="loc_us-gaap_OperatingIncomeLoss_9894e895-7695-402a-9f03-08d60b7373b8" xlink:type="locator" />
    <link:calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_a53f52a4-7a54-3283-f734-95683c76ebff" xlink:to="loc_us-gaap_OperatingIncomeLoss_9894e895-7695-402a-9f03-08d60b7373b8" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_GrossProfit" xlink:label="loc_us-gaap_GrossProfit_aaae347c-fa91-e2c3-dd05-1b008b13fd0c" xlink:type="locator" />
    <link:calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingIncomeLoss_9894e895-7695-402a-9f03-08d60b7373b8" xlink:to="loc_us-gaap_GrossProfit_aaae347c-fa91-e2c3-dd05-1b008b13fd0c" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_Revenues" xlink:label="loc_us-gaap_Revenues_33f76b65-31cf-be04-0849-25af345446d5" xlink:type="locator" />
    <link:calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_GrossProfit_aaae347c-fa91-e2c3-dd05-1b008b13fd0c" xlink:to="loc_us-gaap_Revenues_33f76b65-31cf-be04-0849-25af345446d5" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_CostOfRevenue" xlink:label="loc_us-gaap_CostOfRevenue_a2b142e3-b3f3-b7b6-6428-5812971ab980" xlink:type="locator" />
    <link:calculationArc order="2" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_GrossProfit_aaae347c-fa91-e2c3-dd05-1b008b13fd0c" xlink:to="loc_us-gaap_CostOfRevenue_a2b142e3-b3f3-b7b6-6428-5812971ab980" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_OperatingExpenses" xlink:label="loc_us-gaap_OperatingExpenses_23a4428c-a5e3-3b9c-fc88-04ae5be27ab3" xlink:type="locator" />
    <link:calculationArc order="2" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingIncomeLoss_9894e895-7695-402a-9f03-08d60b7373b8" xlink:to="loc_us-gaap_OperatingExpenses_23a4428c-a5e3-3b9c-fc88-04ae5be27ab3" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_SellingGeneralAndAdministrativeExpense" xlink:label="loc_us-gaap_SellingGeneralAndAdministrativeExpense_29ad42f4-24c5-90ea-4492-fdb757d66249" xlink:type="locator" />
    <link:calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingExpenses_23a4428c-a5e3-3b9c-fc88-04ae5be27ab3" xlink:to="loc_us-gaap_SellingGeneralAndAdministrativeExpense_29ad42f4-24c5-90ea-4492-fdb757d66249" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_ResearchAndDevelopmentExpense" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpense_25ae5ce2-b413-b975-9a84-c7503d2496cf" xlink:type="locator" />
    <link:calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingExpenses_23a4428c-a5e3-3b9c-fc88-04ae5be27ab3" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpense_25ae5ce2-b413-b975-9a84-c7503d2496cf" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_NonoperatingIncomeExpense" xlink:label="loc_us-gaap_NonoperatingIncomeExpense_9b894abb-bf85-2810-9d9d-c92a9b0f56f1" xlink:type="locator" />
    <link:calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_a53f52a4-7a54-3283-f734-95683c76ebff" xlink:to="loc_us-gaap_NonoperatingIncomeExpense_9b894abb-bf85-2810-9d9d-c92a9b0f56f1" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_ComprehensiveIncomeNetOfTax" xlink:label="loc_us-gaap_ComprehensiveIncomeNetOfTax_499015ce-2160-aed2-85a3-080e8c4c6cdf" xlink:type="locator" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_OtherComprehensiveIncomeUnrealizedHoldingGainLossOnSecuritiesArisingDuringPeriodNetOfTax" xlink:label="loc_us-gaap_OtherComprehensiveIncomeUnrealizedHoldingGainLossOnSecuritiesArisingDuringPeriodNetOfTax_57e8ae74-d8b6-c629-9bee-2d0929cd3999" xlink:type="locator" />
    <link:calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_499015ce-2160-aed2-85a3-080e8c4c6cdf" xlink:to="loc_us-gaap_OtherComprehensiveIncomeUnrealizedHoldingGainLossOnSecuritiesArisingDuringPeriodNetOfTax_57e8ae74-d8b6-c629-9bee-2d0929cd3999" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_OtherComprehensiveIncomeLossReclassificationAdjustmentFromAOCIForWritedownOfSecuritiesNetOfTax" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossReclassificationAdjustmentFromAOCIForWritedownOfSecuritiesNetOfTax_c5412587-e395-30bd-586d-2fbe60ebfbc0" xlink:type="locator" />
    <link:calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_499015ce-2160-aed2-85a3-080e8c4c6cdf" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossReclassificationAdjustmentFromAOCIForWritedownOfSecuritiesNetOfTax_c5412587-e395-30bd-586d-2fbe60ebfbc0" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_NetIncomeLoss" xlink:label="loc_us-gaap_NetIncomeLoss_641b1cab-a2b3-2e0e-8389-1455ff3f8581" xlink:type="locator" />
    <link:calculationArc order="3" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_499015ce-2160-aed2-85a3-080e8c4c6cdf" xlink:to="loc_us-gaap_NetIncomeLoss_641b1cab-a2b3-2e0e-8389-1455ff3f8581" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_ProfitLoss" xlink:label="loc_us-gaap_ProfitLoss_adacf05e-7e15-bd60-3e53-87432885dc29" xlink:type="locator" />
    <link:calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_641b1cab-a2b3-2e0e-8389-1455ff3f8581" xlink:to="loc_us-gaap_ProfitLoss_adacf05e-7e15-bd60-3e53-87432885dc29" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_NetIncomeLossAttributableToNoncontrollingInterest" xlink:label="loc_us-gaap_NetIncomeLossAttributableToNoncontrollingInterest_baabbf0a-644b-708a-c310-1211f9af2775" xlink:type="locator" />
    <link:calculationArc order="2" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_641b1cab-a2b3-2e0e-8389-1455ff3f8581" xlink:to="loc_us-gaap_NetIncomeLossAttributableToNoncontrollingInterest_baabbf0a-644b-708a-c310-1211f9af2775" xlink:type="arc" />
  </link:calculationLink>
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/CondensedConsolidatedStatementsOfOperationsAndComprehensiveLossCalc2" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/DocumentAndEntityInformation" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/FairValueMeasurement" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/FairValueMeasurementDetails" xlink:type="extended">
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_Investments" xlink:label="loc_us-gaap_Investments_b3499a0f-b913-a4fd-9d0e-0ba0eb53fbb6" xlink:type="locator" />
    <link:loc xlink:href="astc-20170930.xsd#astc_MutualFundsCarringValue" xlink:label="loc_astc_MutualFundsCarringValue_42a9b0ae-fb3b-5498-3733-ec786a13892f" xlink:type="locator" />
    <link:calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Investments_b3499a0f-b913-a4fd-9d0e-0ba0eb53fbb6" xlink:to="loc_astc_MutualFundsCarringValue_42a9b0ae-fb3b-5498-3733-ec786a13892f" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_HeldToMaturitySecuritiesDebtMaturitiesWithinOneYearNetCarryingAmount" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesWithinOneYearNetCarryingAmount_01f5fca0-0070-5249-0eb7-1cb84a39b7e6" xlink:type="locator" />
    <link:calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Investments_b3499a0f-b913-a4fd-9d0e-0ba0eb53fbb6" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesWithinOneYearNetCarryingAmount_01f5fca0-0070-5249-0eb7-1cb84a39b7e6" xlink:type="arc" />
    <link:loc xlink:href="astc-20170930.xsd#astc_HeldtomaturitySecuritiesDebtMaturitiesafterOneThroughThreeYearsNetCarryingAmount" xlink:label="loc_astc_HeldtomaturitySecuritiesDebtMaturitiesafterOneThroughThreeYearsNetCarryingAmount_f7409249-9751-b6a0-b52f-ec786a932c1d" xlink:type="locator" />
    <link:calculationArc order="3" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Investments_b3499a0f-b913-a4fd-9d0e-0ba0eb53fbb6" xlink:to="loc_astc_HeldtomaturitySecuritiesDebtMaturitiesafterOneThroughThreeYearsNetCarryingAmount_f7409249-9751-b6a0-b52f-ec786a932c1d" xlink:type="arc" />
    <link:loc xlink:href="astc-20170930.xsd#astc_HeldToMaturitySecuritiesDebtMaturities91To360DaysNetCarryingAmount" xlink:label="loc_astc_HeldToMaturitySecuritiesDebtMaturities91To360DaysNetCarryingAmount_1d9e6acb-3a7c-2d84-5160-ec78693fc0d3" xlink:type="locator" />
    <link:calculationArc order="4" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Investments_b3499a0f-b913-a4fd-9d0e-0ba0eb53fbb6" xlink:to="loc_astc_HeldToMaturitySecuritiesDebtMaturities91To360DaysNetCarryingAmount_1d9e6acb-3a7c-2d84-5160-ec78693fc0d3" xlink:type="arc" />
    <link:loc xlink:href="astc-20170930.xsd#astc_HeldToMaturitySecuritiesDebtMaturitiesOver360DaysNetCarryingAmount" xlink:label="loc_astc_HeldToMaturitySecuritiesDebtMaturitiesOver360DaysNetCarryingAmount_9974baee-641f-d1fb-9b78-ec7868f91562" xlink:type="locator" />
    <link:calculationArc order="5" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Investments_b3499a0f-b913-a4fd-9d0e-0ba0eb53fbb6" xlink:to="loc_astc_HeldToMaturitySecuritiesDebtMaturitiesOver360DaysNetCarryingAmount_9974baee-641f-d1fb-9b78-ec7868f91562" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_InvestmentsFairValueDisclosure" xlink:label="loc_us-gaap_InvestmentsFairValueDisclosure_b851ddfc-ce9b-3407-931e-669b0dd9719b" xlink:type="locator" />
    <link:loc xlink:href="astc-20170930.xsd#astc_MutualFundsFairValue" xlink:label="loc_astc_MutualFundsFairValue_e90a5d5e-a143-91c1-9a6e-ec7868db8837" xlink:type="locator" />
    <link:calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InvestmentsFairValueDisclosure_b851ddfc-ce9b-3407-931e-669b0dd9719b" xlink:to="loc_astc_MutualFundsFairValue_e90a5d5e-a143-91c1-9a6e-ec7868db8837" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_HeldToMaturitySecuritiesDebtMaturitiesWithinOneYearFairValue" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesWithinOneYearFairValue_837d1260-daa2-488c-64c8-2e7be5aee8de" xlink:type="locator" />
    <link:calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InvestmentsFairValueDisclosure_b851ddfc-ce9b-3407-931e-669b0dd9719b" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesWithinOneYearFairValue_837d1260-daa2-488c-64c8-2e7be5aee8de" xlink:type="arc" />
    <link:loc xlink:href="astc-20170930.xsd#astc_HeldtomaturitySecuritiesDebtMaturitiesafterOneThroughThreeYearsFairValue" xlink:label="loc_astc_HeldtomaturitySecuritiesDebtMaturitiesafterOneThroughThreeYearsFairValue_fe6da75c-1bf6-3cb2-6fd0-ec786aa3fa2c" xlink:type="locator" />
    <link:calculationArc order="3" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InvestmentsFairValueDisclosure_b851ddfc-ce9b-3407-931e-669b0dd9719b" xlink:to="loc_astc_HeldtomaturitySecuritiesDebtMaturitiesafterOneThroughThreeYearsFairValue_fe6da75c-1bf6-3cb2-6fd0-ec786aa3fa2c" xlink:type="arc" />
    <link:loc xlink:href="astc-20170930.xsd#astc_HeldToMaturitySecuritiesDebtMaturities91To360DaysFairValue" xlink:label="loc_astc_HeldToMaturitySecuritiesDebtMaturities91To360DaysFairValue_bd8125c1-3955-967b-2a7c-ec7869072447" xlink:type="locator" />
    <link:calculationArc order="4" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InvestmentsFairValueDisclosure_b851ddfc-ce9b-3407-931e-669b0dd9719b" xlink:to="loc_astc_HeldToMaturitySecuritiesDebtMaturities91To360DaysFairValue_bd8125c1-3955-967b-2a7c-ec7869072447" xlink:type="arc" />
    <link:loc xlink:href="astc-20170930.xsd#astc_HeldToMaturitySecuritiesDebtMaturitiesOver360DaysFairValue" xlink:label="loc_astc_HeldToMaturitySecuritiesDebtMaturitiesOver360DaysFairValue_63974560-5234-e5ce-e155-ec78696360a5" xlink:type="locator" />
    <link:calculationArc order="5" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InvestmentsFairValueDisclosure_b851ddfc-ce9b-3407-931e-669b0dd9719b" xlink:to="loc_astc_HeldToMaturitySecuritiesDebtMaturitiesOver360DaysFairValue_63974560-5234-e5ce-e155-ec78696360a5" xlink:type="arc" />
  </link:calculationLink>
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/FairValueMeasurementTables" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/GeneralInformation" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/GeneralInformationDetails" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/GeneralInformationPolicies" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/GoingConcernDetails" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/GoingConcernGoingConcern" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/IncomeTaxes" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/IncomeTaxesDetails" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/Inventory" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/InventoryDetails" xlink:type="extended">
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_InventoryNet" xlink:label="loc_us-gaap_InventoryNet_7eab44cf-1ea1-ca2a-420f-21f7b5343fc3" xlink:type="locator" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_InventoryRawMaterialsNetOfReserves" xlink:label="loc_us-gaap_InventoryRawMaterialsNetOfReserves_d8866e11-7330-6a9a-9ba0-56d88707d8ec" xlink:type="locator" />
    <link:calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InventoryNet_7eab44cf-1ea1-ca2a-420f-21f7b5343fc3" xlink:to="loc_us-gaap_InventoryRawMaterialsNetOfReserves_d8866e11-7330-6a9a-9ba0-56d88707d8ec" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_InventoryWorkInProcessNetOfReserves" xlink:label="loc_us-gaap_InventoryWorkInProcessNetOfReserves_a3451b87-a72a-9fff-128b-969b95aecb3a" xlink:type="locator" />
    <link:calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InventoryNet_7eab44cf-1ea1-ca2a-420f-21f7b5343fc3" xlink:to="loc_us-gaap_InventoryWorkInProcessNetOfReserves_a3451b87-a72a-9fff-128b-969b95aecb3a" xlink:type="arc" />
  </link:calculationLink>
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/InventoryTables" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/Investments" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/InvestmentsAvailableForSaleSecuritiesDetails" xlink:type="extended">
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_AvailableForSaleSecuritiesAmortizedCost" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesAmortizedCost_30dc6014-97a8-c9af-5d13-c233c1983f7f" xlink:type="locator" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_AvailableForSaleSecuritiesAccumulatedGrossUnrealizedGainBeforeTax" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesAccumulatedGrossUnrealizedGainBeforeTax_1932fd43-f07d-5e04-493b-eca2ebb13c97" xlink:type="locator" />
    <link:calculationArc order="1" weight="-1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesAmortizedCost_30dc6014-97a8-c9af-5d13-c233c1983f7f" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesAccumulatedGrossUnrealizedGainBeforeTax_1932fd43-f07d-5e04-493b-eca2ebb13c97" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_AvailableForSaleSecuritiesAccumulatedGrossUnrealizedLossBeforeTax" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesAccumulatedGrossUnrealizedLossBeforeTax_e96beffd-8ab7-62f8-402b-cfa1adccd68f" xlink:type="locator" />
    <link:calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesAmortizedCost_30dc6014-97a8-c9af-5d13-c233c1983f7f" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesAccumulatedGrossUnrealizedLossBeforeTax_e96beffd-8ab7-62f8-402b-cfa1adccd68f" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_AvailableForSaleSecurities" xlink:label="loc_us-gaap_AvailableForSaleSecurities_d444402b-921b-e0fa-119c-908ab66850af" xlink:type="locator" />
    <link:calculationArc order="3" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesAmortizedCost_30dc6014-97a8-c9af-5d13-c233c1983f7f" xlink:to="loc_us-gaap_AvailableForSaleSecurities_d444402b-921b-e0fa-119c-908ab66850af" xlink:type="arc" />
  </link:calculationLink>
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/InvestmentsCarryingValueDetails" xlink:type="extended">
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_HeldToMaturitySecurities" xlink:label="loc_us-gaap_HeldToMaturitySecurities_3bb8bc81-af51-9945-0a22-cc4527e07a17" xlink:type="locator" />
    <link:loc xlink:href="astc-20170930.xsd#astc_HeldToMaturitySecuritiesDebtMaturitiesOver360DaysNetCarryingAmount" xlink:label="loc_astc_HeldToMaturitySecuritiesDebtMaturitiesOver360DaysNetCarryingAmount_9974baee-641f-d1fb-9b78-ec7868f91562" xlink:type="locator" />
    <link:calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_HeldToMaturitySecurities_3bb8bc81-af51-9945-0a22-cc4527e07a17" xlink:to="loc_astc_HeldToMaturitySecuritiesDebtMaturitiesOver360DaysNetCarryingAmount_9974baee-641f-d1fb-9b78-ec7868f91562" xlink:type="arc" />
    <link:loc xlink:href="astc-20170930.xsd#astc_HeldtomaturitySecuritiesDebtMaturitiesafterOneThroughThreeYearsNetCarryingAmount" xlink:label="loc_astc_HeldtomaturitySecuritiesDebtMaturitiesafterOneThroughThreeYearsNetCarryingAmount_f7409249-9751-b6a0-b52f-ec786a932c1d" xlink:type="locator" />
    <link:calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_HeldToMaturitySecurities_3bb8bc81-af51-9945-0a22-cc4527e07a17" xlink:to="loc_astc_HeldtomaturitySecuritiesDebtMaturitiesafterOneThroughThreeYearsNetCarryingAmount_f7409249-9751-b6a0-b52f-ec786a932c1d" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_AvailableForSaleSecuritiesAndHeldToMaturitySecurities" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesAndHeldToMaturitySecurities_55c5f76e-f56e-3fcb-efbd-87adabf617dd" xlink:type="locator" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_AvailableForSaleSecurities" xlink:label="loc_us-gaap_AvailableForSaleSecurities_d444402b-921b-e0fa-119c-908ab66850af" xlink:type="locator" />
    <link:calculationArc order="1" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesAndHeldToMaturitySecurities_55c5f76e-f56e-3fcb-efbd-87adabf617dd" xlink:to="loc_us-gaap_AvailableForSaleSecurities_d444402b-921b-e0fa-119c-908ab66850af" xlink:type="arc" />
    <link:loc xlink:href="astc-20170930.xsd#astc_HeldToMaturitySecuritiesDebtMaturities1To90DaysNetCarryingAmount" xlink:label="loc_astc_HeldToMaturitySecuritiesDebtMaturities1To90DaysNetCarryingAmount_65f3a928-96d1-75ac-5ddc-ec786a3e9902" xlink:type="locator" />
    <link:calculationArc order="2" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesAndHeldToMaturitySecurities_55c5f76e-f56e-3fcb-efbd-87adabf617dd" xlink:to="loc_astc_HeldToMaturitySecuritiesDebtMaturities1To90DaysNetCarryingAmount_65f3a928-96d1-75ac-5ddc-ec786a3e9902" xlink:type="arc" />
    <link:loc xlink:href="astc-20170930.xsd#astc_HeldToMaturitySecuritiesDebtMaturities91To360DaysNetCarryingAmount" xlink:label="loc_astc_HeldToMaturitySecuritiesDebtMaturities91To360DaysNetCarryingAmount_1d9e6acb-3a7c-2d84-5160-ec78693fc0d3" xlink:type="locator" />
    <link:calculationArc order="3" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesAndHeldToMaturitySecurities_55c5f76e-f56e-3fcb-efbd-87adabf617dd" xlink:to="loc_astc_HeldToMaturitySecuritiesDebtMaturities91To360DaysNetCarryingAmount_1d9e6acb-3a7c-2d84-5160-ec78693fc0d3" xlink:type="arc" />
    <link:loc xlink:href="http://xbrl.fasb.org/us-gaap/2017/elts/us-gaap-2017-01-31.xsd#us-gaap_HeldToMaturitySecuritiesDebtMaturitiesWithinOneYearNetCarryingAmount" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesWithinOneYearNetCarryingAmount_01f5fca0-0070-5249-0eb7-1cb84a39b7e6" xlink:type="locator" />
    <link:calculationArc order="4" weight="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesAndHeldToMaturitySecurities_55c5f76e-f56e-3fcb-efbd-87adabf617dd" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesWithinOneYearNetCarryingAmount_01f5fca0-0070-5249-0eb7-1cb84a39b7e6" xlink:type="arc" />
  </link:calculationLink>
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/InvestmentsTables" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/NetLossPerShare" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/NetLossPerShareBasicAndDilutedComputationDetails" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/NetLossPerShareNarrativeDetails" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/NetLossPerShareTables" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/NoncontrollingInterest" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/NoncontrollingInterestChangesInEquityDetails" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/NoncontrollingInterestContributionsDetails" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/NoncontrollingInterestNarrativeDetails" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/NoncontrollingInterestTables" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/OtherComprehensiveLoss" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/OtherComprehensiveLossDetails" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/OtherComprehensiveLossTables" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/RevenueRecognition" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/RevenueRecognitionDetails" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/SegmentInformation" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/SegmentInformationNarrativeDetails" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/SegmentInformationScheduleOfSegmentsDetails" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/SegmentInformationTables" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/SubsequentEvents" xlink:type="extended" />
  <link:calculationLink xlink:role="http://www.astrotechcorp.com/role/SubsequentEventsDetails" xlink:type="extended" />
</link:linkbase>
