<SEC-DOCUMENT>0001415889-25-020265.txt : 20250724
<SEC-HEADER>0001415889-25-020265.hdr.sgml : 20250724
<ACCEPTANCE-DATETIME>20250724193421
ACCESSION NUMBER:		0001415889-25-020265
CONFORMED SUBMISSION TYPE:	4
PUBLIC DOCUMENT COUNT:		1
CONFORMED PERIOD OF REPORT:	20250723
FILED AS OF DATE:		20250724
DATE AS OF CHANGE:		20250724

REPORTING-OWNER:	

	OWNER DATA:	
		COMPANY CONFORMED NAME:			Ways Kirk
		CENTRAL INDEX KEY:			0001869391
		ORGANIZATION NAME:           	

	FILING VALUES:
		FORM TYPE:		4
		SEC ACT:		1934 Act
		SEC FILE NUMBER:	001-39594
		FILM NUMBER:		251148199

	MAIL ADDRESS:	
		STREET 1:		C/O 2001 JUNIPERO SERRA BOULEVARD
		STREET 2:		SUITE 540
		CITY:			DALY CITY
		STATE:			CA
		ZIP:			94014

ISSUER:		

	COMPANY DATA:	
		COMPANY CONFORMED NAME:			SPRUCE BIOSCIENCES, INC.
		CENTRAL INDEX KEY:			0001683553
		STANDARD INDUSTRIAL CLASSIFICATION:	PHARMACEUTICAL PREPARATIONS [2834]
		ORGANIZATION NAME:           	03 Life Sciences
		EIN:				812154263
		STATE OF INCORPORATION:			DE
		FISCAL YEAR END:			1231

	BUSINESS ADDRESS:	
		STREET 1:		611 GATEWAY BOULEVARD, SUITE 740
		CITY:			SOUTH SAN FRANCISCO
		STATE:			CA
		ZIP:			94080
		BUSINESS PHONE:		(415) 655-4168

	MAIL ADDRESS:	
		STREET 1:		611 GATEWAY BOULEVARD, SUITE 740
		CITY:			SOUTH SAN FRANCISCO
		STATE:			CA
		ZIP:			94080
</SEC-HEADER>
<DOCUMENT>
<TYPE>4
<SEQUENCE>1
<FILENAME>form4-07242025_110718.xml
<TEXT>
<XML>
<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0508</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2025-07-23</periodOfReport>

    <issuer>
        <issuerCik>0001683553</issuerCik>
        <issuerName>SPRUCE BIOSCIENCES, INC.</issuerName>
        <issuerTradingSymbol>SPRB</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001869391</rptOwnerCik>
            <rptOwnerName>Ways Kirk</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O SPRUCE BIOSCIENCES, INC.</rptOwnerStreet1>
            <rptOwnerStreet2>611 GATEWAY BOULEVARD, SUITE 740</rptOwnerStreet2>
            <rptOwnerCity>SOUTH SAN FRANCISCO</rptOwnerCity>
            <rptOwnerState>CA</rptOwnerState>
            <rptOwnerZipCode>94080</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>true</isDirector>
            <isOfficer>true</isOfficer>
            <isTenPercentOwner>false</isTenPercentOwner>
            <isOther>false</isOther>
            <officerTitle>Interim Chief Medical Officer</officerTitle>
            <otherText></otherText>
        </reportingOwnerRelationship>
    </reportingOwner>

    <aff10b5One>0</aff10b5One>

    <nonDerivativeTable></nonDerivativeTable>

    <derivativeTable>
        <derivativeTransaction>
            <securityTitle>
                <value>Stock Option (Right to Buy)</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <value>0.1235</value>
            </conversionOrExercisePrice>
            <transactionDate>
                <value>2025-07-23</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>A</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>30000</value>
                </transactionShares>
                <transactionPricePerShare>
                    <value>0</value>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>A</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <exerciseDate>
                <footnoteId id="F1"/>
            </exerciseDate>
            <expirationDate>
                <value>2035-07-22</value>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>30000</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>30000</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value></value>
                </natureOfOwnership>
            </ownershipNature>
        </derivativeTransaction>
    </derivativeTable>

    <footnotes>
        <footnote id="F1">The shares vest on the first anniversary of the Grant Date, provided that the Option will in any case be fully vested on the date of Company's 2026 annual stockholder meeting, subject to the Reporting Person's Continuous Service (as defined in the 2020 Equity Incentive Plan (the &quot;Plan&quot;)) through such vesting date and will vest in full upon a Change in Control (as defined in the Plan).</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ Samir Gharib, Attorney-in-Fact</signatureName>
        <signatureDate>2025-07-24</signatureDate>
    </ownerSignature>
</ownershipDocument>
</XML>
</TEXT>
</DOCUMENT>
</SEC-DOCUMENT>
