<?xml version="1.0" encoding="utf-8"?>
<!--XBRL document created with Toppan Merrill Bridge  10.9.0.3 -->
<!--Based on XBRL 2.1-->
<!--Created on: 2/28/2025 9:07:14 PM-->
<!--Modified on: 2/28/2025 9:07:14 PM-->
<link:linkbase xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd" xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xbrli="http://www.xbrl.org/2003/instance">
  <roleRef xlink:type="simple" xlink:href="bno-20241231.xsd#StatementStatementsOfFinancialCondition" roleURI="http://www.unitedstatesbrentoilfund.com/role/StatementStatementsOfFinancialCondition" xmlns="http://www.xbrl.org/2003/linkbase" />
  <roleRef xlink:type="simple" xlink:href="bno-20241231.xsd#StatementStatementsOfOperations" roleURI="http://www.unitedstatesbrentoilfund.com/role/StatementStatementsOfOperations" xmlns="http://www.xbrl.org/2003/linkbase" />
  <roleRef xlink:type="simple" xlink:href="bno-20241231.xsd#StatementCondensedStatementsOfCashFlowsUnauditedCalc2" roleURI="http://www.unitedstatesbrentoilfund.com/role/StatementCondensedStatementsOfCashFlowsUnauditedCalc2" xmlns="http://www.xbrl.org/2003/linkbase" />
  <roleRef xlink:type="simple" xlink:href="bno-20241231.xsd#StatementStatementsOfCashFlows" roleURI="http://www.unitedstatesbrentoilfund.com/role/StatementStatementsOfCashFlows" xmlns="http://www.xbrl.org/2003/linkbase" />
  <roleRef xlink:type="simple" xlink:href="bno-20241231.xsd#DisclosureFinancialHighlightsDetails" roleURI="http://www.unitedstatesbrentoilfund.com/role/DisclosureFinancialHighlightsDetails" xmlns="http://www.xbrl.org/2003/linkbase" />
  <link:arcroleRef arcroleURI="https://xbrl.org/2023/arcrole/summation-item" xlink:type="simple" xlink:href="https://www.xbrl.org/2023/calculation-1.1.xsd#summation-item" />
  <calculationLink xlink:role="http://www.unitedstatesbrentoilfund.com/role/StatementStatementsOfFinancialCondition" xlink:type="extended" xlink:title="995200100 - Statement - Statements of Financial Condition" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Assets" xlink:label="us-gaap_Assets" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue" xlink:label="us-gaap_CashAndCashEquivalentsAtCarryingValue_638763736328450966" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_Assets" xlink:to="us-gaap_CashAndCashEquivalentsAtCarryingValue_638763736328450966" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RestrictedCashAndCashEquivalentsAtCarryingValue" xlink:label="us-gaap_RestrictedCashAndCashEquivalentsAtCarryingValue_638763736328450966" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_Assets" xlink:to="us-gaap_RestrictedCashAndCashEquivalentsAtCarryingValue_638763736328450966" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="bno-20241231.xsd#bno_UnrealizedGainLossOnOpenCommodityFuturesContractsCurrentAndNoncurrent" xlink:label="bno_UnrealizedGainLossOnOpenCommodityFuturesContractsCurrentAndNoncurrent_638763736328450966" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_Assets" xlink:to="bno_UnrealizedGainLossOnOpenCommodityFuturesContractsCurrentAndNoncurrent_638763736328450966" order="3" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DividendsReceivable" xlink:label="us-gaap_DividendsReceivable_638763736328450966" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_Assets" xlink:to="us-gaap_DividendsReceivable_638763736328450966" order="4" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InterestReceivable" xlink:label="us-gaap_InterestReceivable_638763736328450966" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_Assets" xlink:to="us-gaap_InterestReceivable_638763736328450966" order="5" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PrepaidInsurance" xlink:label="us-gaap_PrepaidInsurance_638763736328450966" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_Assets" xlink:to="us-gaap_PrepaidInsurance_638763736328450966" order="6" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LiabilitiesAndStockholdersEquity" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquity" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Liabilities" xlink:label="us-gaap_Liabilities_638763736328460942" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity" xlink:to="us-gaap_Liabilities_638763736328460942" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherLiabilities" xlink:label="us-gaap_OtherLiabilities_638763736328460942" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_Liabilities_638763736328460942" xlink:to="us-gaap_OtherLiabilities_638763736328460942" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccruedProfessionalFeesCurrentAndNoncurrent" xlink:label="us-gaap_AccruedProfessionalFeesCurrentAndNoncurrent_638763736328460942" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_Liabilities_638763736328460942" xlink:to="us-gaap_AccruedProfessionalFeesCurrentAndNoncurrent_638763736328460942" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommissionsPayableToBrokerDealersAndClearingOrganizations" xlink:label="us-gaap_CommissionsPayableToBrokerDealersAndClearingOrganizations_638763736328460942" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_Liabilities_638763736328460942" xlink:to="us-gaap_CommissionsPayableToBrokerDealersAndClearingOrganizations_638763736328460942" order="3" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ManagementFeePayable" xlink:label="us-gaap_ManagementFeePayable_638763736328460942" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_Liabilities_638763736328460942" xlink:to="us-gaap_ManagementFeePayable_638763736328460942" order="4" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PartnersCapital" xlink:label="us-gaap_PartnersCapital_638763736328470940" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity" xlink:to="us-gaap_PartnersCapital_638763736328470940" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_GeneralPartnersCapitalAccount" xlink:label="us-gaap_GeneralPartnersCapitalAccount_638763736328470940" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_PartnersCapital_638763736328470940" xlink:to="us-gaap_GeneralPartnersCapitalAccount_638763736328470940" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LimitedPartnersCapitalAccount" xlink:label="us-gaap_LimitedPartnersCapitalAccount_638763736328470940" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_PartnersCapital_638763736328470940" xlink:to="us-gaap_LimitedPartnersCapitalAccount_638763736328470940" order="2" use="optional" weight="1" />
  </calculationLink>
  <calculationLink xlink:role="http://www.unitedstatesbrentoilfund.com/role/StatementStatementsOfOperations" xlink:type="extended" xlink:title="995200300 - Statement - Statements of Operations" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetIncomeLoss" xlink:label="loc_us-gaap_NetIncomeLoss" />
    <loc xlink:type="locator" xlink:href="bno-20241231.xsd#bno_TotalIncomeLoss" xlink:label="bno_TotalIncomeLoss_638763736328470940" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss" xlink:to="bno_TotalIncomeLoss_638763736328470940" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_GainLossOnSaleOfInvestments" xlink:label="us-gaap_GainLossOnSaleOfInvestments_638763736328470940" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="bno_TotalIncomeLoss_638763736328470940" xlink:to="us-gaap_GainLossOnSaleOfInvestments_638763736328470940" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_UnrealizedGainLossOnInvestments" xlink:label="us-gaap_UnrealizedGainLossOnInvestments_638763736328480941" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="bno_TotalIncomeLoss_638763736328470940" xlink:to="us-gaap_UnrealizedGainLossOnInvestments_638763736328480941" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DividendIncomeOperating" xlink:label="us-gaap_DividendIncomeOperating_638763736328480941" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="bno_TotalIncomeLoss_638763736328470940" xlink:to="us-gaap_DividendIncomeOperating_638763736328480941" order="3" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InterestIncomeOperating" xlink:label="us-gaap_InterestIncomeOperating_638763736328480941" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="bno_TotalIncomeLoss_638763736328470940" xlink:to="us-gaap_InterestIncomeOperating_638763736328480941" order="4" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherIncome" xlink:label="us-gaap_OtherIncome_638763736328480941" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="bno_TotalIncomeLoss_638763736328470940" xlink:to="us-gaap_OtherIncome_638763736328480941" order="5" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="bno-20241231.xsd#bno_TotalExpenses" xlink:label="bno_TotalExpenses_638763736328480941" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss" xlink:to="bno_TotalExpenses_638763736328480941" order="2" use="optional" weight="-1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ManagementFeeExpense" xlink:label="us-gaap_ManagementFeeExpense_638763736328480941" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="bno_TotalExpenses_638763736328480941" xlink:to="us-gaap_ManagementFeeExpense_638763736328480941" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProfessionalFees" xlink:label="us-gaap_ProfessionalFees_638763736328480941" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="bno_TotalExpenses_638763736328480941" xlink:to="us-gaap_ProfessionalFees_638763736328480941" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FloorBrokerageExchangeAndClearanceFees" xlink:label="us-gaap_FloorBrokerageExchangeAndClearanceFees_638763736328490933" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="bno_TotalExpenses_638763736328480941" xlink:to="us-gaap_FloorBrokerageExchangeAndClearanceFees_638763736328490933" order="3" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NoninterestExpenseDirectorsFees" xlink:label="us-gaap_NoninterestExpenseDirectorsFees_638763736328490933" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="bno_TotalExpenses_638763736328480941" xlink:to="us-gaap_NoninterestExpenseDirectorsFees_638763736328490933" order="4" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="bno-20241231.xsd#bno_LicenseFeesExpenses" xlink:label="bno_LicenseFeesExpenses_638763736328490933" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="bno_TotalExpenses_638763736328480941" xlink:to="bno_LicenseFeesExpenses_638763736328490933" order="5" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InvestmentCompanyRegistrationExpense" xlink:label="us-gaap_InvestmentCompanyRegistrationExpense_638763736328490933" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="bno_TotalExpenses_638763736328480941" xlink:to="us-gaap_InvestmentCompanyRegistrationExpense_638763736328490933" order="6" use="optional" weight="1" />
  </calculationLink>
  <calculationLink xlink:role="http://www.unitedstatesbrentoilfund.com/role/StatementCondensedStatementsOfCashFlowsUnauditedCalc2" xlink:type="extended" xlink:title="00500 - Statement - Condensed Statements of Cash Flows (Unaudited) (Calc 2)" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents" xlink:label="us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_1" />
    <loc xlink:type="locator" xlink:href="bno-20241231.xsd#bno_CashAndCashEquivalentsInEquityInTradingAccounts" xlink:label="bno_CashAndCashEquivalentsInEquityInTradingAccounts_638763736328490933" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_1" xlink:to="bno_CashAndCashEquivalentsInEquityInTradingAccounts_638763736328490933" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue" xlink:label="us-gaap_CashAndCashEquivalentsAtCarryingValue_638763736328500937" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_1" xlink:to="us-gaap_CashAndCashEquivalentsAtCarryingValue_638763736328500937" order="2" use="optional" weight="1" />
  </calculationLink>
  <calculationLink xlink:role="http://www.unitedstatesbrentoilfund.com/role/StatementStatementsOfCashFlows" xlink:type="extended" xlink:title="995200500 - Statement - Statements of Cash Flows" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseExcludingExchangeRateEffect" xlink:label="us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseExcludingExchangeRateEffect" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities" xlink:label="us-gaap_NetCashProvidedByUsedInFinancingActivities_638763736328500937" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseExcludingExchangeRateEffect" xlink:to="us-gaap_NetCashProvidedByUsedInFinancingActivities_638763736328500937" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromPartnershipContribution" xlink:label="us-gaap_ProceedsFromPartnershipContribution_638763736328500937" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_NetCashProvidedByUsedInFinancingActivities_638763736328500937" xlink:to="us-gaap_ProceedsFromPartnershipContribution_638763736328500937" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="bno-20241231.xsd#bno_PaymentsToPartnershipRedemption" xlink:label="bno_PaymentsToPartnershipRedemption_638763736328500937" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_NetCashProvidedByUsedInFinancingActivities_638763736328500937" xlink:to="bno_PaymentsToPartnershipRedemption_638763736328500937" order="2" use="optional" weight="-1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities" xlink:label="us-gaap_NetCashProvidedByUsedInOperatingActivities_638763736328500937" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseExcludingExchangeRateEffect" xlink:to="us-gaap_NetCashProvidedByUsedInOperatingActivities_638763736328500937" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetIncomeLoss" xlink:label="us-gaap_NetIncomeLoss_638763736328500937" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivities_638763736328500937" xlink:to="us-gaap_NetIncomeLoss_638763736328500937" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_UnrealizedGainLossOnDerivativesAndCommodityContracts" xlink:label="us-gaap_UnrealizedGainLossOnDerivativesAndCommodityContracts_638763736328510938" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivities_638763736328500937" xlink:to="us-gaap_UnrealizedGainLossOnDerivativesAndCommodityContracts_638763736328510938" order="2" use="optional" weight="-1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInDueFromRelatedPartiesCurrent" xlink:label="us-gaap_IncreaseDecreaseInDueFromRelatedPartiesCurrent_638763736328510938" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivities_638763736328500937" xlink:to="us-gaap_IncreaseDecreaseInDueFromRelatedPartiesCurrent_638763736328510938" order="3" use="optional" weight="-1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInDividendsReceivable" xlink:label="us-gaap_IncreaseDecreaseInDividendsReceivable_638763736328510938" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivities_638763736328500937" xlink:to="us-gaap_IncreaseDecreaseInDividendsReceivable_638763736328510938" order="4" use="optional" weight="-1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInAccruedInterestReceivableNet" xlink:label="us-gaap_IncreaseDecreaseInAccruedInterestReceivableNet_638763736328510938" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivities_638763736328500937" xlink:to="us-gaap_IncreaseDecreaseInAccruedInterestReceivableNet_638763736328510938" order="5" use="optional" weight="-1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInPrepaidInsurance" xlink:label="us-gaap_IncreaseDecreaseInPrepaidInsurance_638763736328520946" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivities_638763736328500937" xlink:to="us-gaap_IncreaseDecreaseInPrepaidInsurance_638763736328520946" order="6" use="optional" weight="-1" priority="3" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInAccountsAndOtherReceivables" xlink:label="us-gaap_IncreaseDecreaseInAccountsAndOtherReceivables_638763736328520946" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivities_638763736328500937" xlink:to="us-gaap_IncreaseDecreaseInAccountsAndOtherReceivables_638763736328520946" order="7" use="optional" weight="-1" priority="3" />
    <loc xlink:type="locator" xlink:href="bno-20241231.xsd#bno_IncreaseDecreaseManagementFeesPayable" xlink:label="bno_IncreaseDecreaseManagementFeesPayable_638763736328520946" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivities_638763736328500937" xlink:to="bno_IncreaseDecreaseManagementFeesPayable_638763736328520946" order="8" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInPayablesToBrokerDealers" xlink:label="us-gaap_IncreaseDecreaseInPayablesToBrokerDealers_638763736328520946" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivities_638763736328500937" xlink:to="us-gaap_IncreaseDecreaseInPayablesToBrokerDealers_638763736328520946" order="9" use="optional" weight="1" priority="1" />
    <loc xlink:type="locator" xlink:href="bno-20241231.xsd#bno_IncreaseDecreaseInRegistrationFeesPayable" xlink:label="bno_IncreaseDecreaseInRegistrationFeesPayable_638763736328520946" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivities_638763736328500937" xlink:to="bno_IncreaseDecreaseInRegistrationFeesPayable_638763736328520946" order="10" use="optional" weight="1" priority="2" />
    <loc xlink:type="locator" xlink:href="bno-20241231.xsd#bno_IncreaseDecreaseInProfessionalFeesPayable" xlink:label="bno_IncreaseDecreaseInProfessionalFeesPayable_638763736328520946" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivities_638763736328500937" xlink:to="bno_IncreaseDecreaseInProfessionalFeesPayable_638763736328520946" order="11" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInDueToOfficersAndStockholders" xlink:label="us-gaap_IncreaseDecreaseInDueToOfficersAndStockholders_638763736328530928" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="us-gaap_NetCashProvidedByUsedInOperatingActivities_638763736328500937" xlink:to="us-gaap_IncreaseDecreaseInDueToOfficersAndStockholders_638763736328530928" order="12" use="optional" weight="1" />
  </calculationLink>
  <calculationLink xlink:role="http://www.unitedstatesbrentoilfund.com/role/DisclosureFinancialHighlightsDetails" xlink:type="extended" xlink:title="99940601 - Disclosure - FINANCIAL HIGHLIGHTS (Details)" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="bno-20241231.xsd#bno_NetIncreaseDecreaseInNetAssetValue" xlink:label="loc_bno_NetIncreaseDecreaseInNetAssetValue" />
    <loc xlink:type="locator" xlink:href="bno-20241231.xsd#bno_TotalIncomePerUnit" xlink:label="bno_TotalIncomePerUnit_638763736328530928" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_bno_NetIncreaseDecreaseInNetAssetValue" xlink:to="bno_TotalIncomePerUnit_638763736328530928" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="bno-20241231.xsd#bno_TotalExpensePerUnit" xlink:label="bno_TotalExpensePerUnit_638763736328530928" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_bno_NetIncreaseDecreaseInNetAssetValue" xlink:to="bno_TotalExpensePerUnit_638763736328530928" order="2" use="optional" weight="1" />
  </calculationLink>
</link:linkbase>
