<?xml version="1.0" encoding="utf-8"?>
<!--Generated by DFIN XBRL Instance Document - http://www.dfinsolutions.com/ - Version R3.0 - on 02-March-2023 [05:10:38] {PM}-->
<linkbase xmlns="http://www.xbrl.org/2003/linkbase" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xlink="http://www.w3.org/1999/xlink" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd" xmlns:xbrldt="http://xbrl.org/2005/xbrldt">
<roleRef xlink:type="simple" xlink:href="aaau-20221231.xsd#StatementsOfCashFlows" roleURI="http://perthmintbullion.com/role/StatementsOfCashFlows" />
<roleRef xlink:type="simple" xlink:href="aaau-20221231.xsd#SchedulesOfInvestmentsSchedulesOfInvestmentsDetails" roleURI="http://perthmintbullion.com/role/SchedulesOfInvestmentsSchedulesOfInvestmentsDetails" />
<roleRef xlink:type="simple" xlink:href="aaau-20221231.xsd#OrganizationDetails" roleURI="http://perthmintbullion.com/role/OrganizationDetails" />
<roleRef xlink:type="simple" xlink:href="aaau-20221231.xsd#StatementsOfAssetsAndLiabilitiesParentheticals" roleURI="http://perthmintbullion.com/role/StatementsOfAssetsAndLiabilitiesParentheticals" />
<roleRef xlink:type="simple" xlink:href="aaau-20221231.xsd#StatementsOfChangesInNetAssets" roleURI="http://perthmintbullion.com/role/StatementsOfChangesInNetAssets" />
<roleRef xlink:type="simple" xlink:href="aaau-20221231.xsd#Organization" roleURI="http://perthmintbullion.com/role/Organization" />
<roleRef xlink:type="simple" xlink:href="aaau-20221231.xsd#SignificantAccountingPolicies" roleURI="http://perthmintbullion.com/role/SignificantAccountingPolicies" />
<roleRef xlink:type="simple" xlink:href="aaau-20221231.xsd#InvestmentInGold" roleURI="http://perthmintbullion.com/role/InvestmentInGold" />
<roleRef xlink:type="simple" xlink:href="aaau-20221231.xsd#RelatedPartiesSponsorTrusteeCustodianAndMarketingFees" roleURI="http://perthmintbullion.com/role/RelatedPartiesSponsorTrusteeCustodianAndMarketingFees" />
<roleRef xlink:type="simple" xlink:href="aaau-20221231.xsd#ConcentrationOfRisk" roleURI="http://perthmintbullion.com/role/ConcentrationOfRisk" />
<roleRef xlink:type="simple" xlink:href="aaau-20221231.xsd#Indemnification" roleURI="http://perthmintbullion.com/role/Indemnification" />
<roleRef xlink:type="simple" xlink:href="aaau-20221231.xsd#SignificantAccountingPoliciesPolicies" roleURI="http://perthmintbullion.com/role/SignificantAccountingPoliciesPolicies" />
<roleRef xlink:type="simple" xlink:href="aaau-20221231.xsd#SignificantAccountingPoliciesScheduleOfChangesInOuncesOfGoldAndRespectiveFairValueDetails" roleURI="http://perthmintbullion.com/role/SignificantAccountingPoliciesScheduleOfChangesInOuncesOfGoldAndRespectiveFairValueDetails" />
<roleRef xlink:type="simple" xlink:href="aaau-20221231.xsd#InvestmentInGoldScheduleOfValuationOfInvestmentsDetails" roleURI="http://perthmintbullion.com/role/InvestmentInGoldScheduleOfValuationOfInvestmentsDetails" />
<roleRef xlink:type="simple" xlink:href="aaau-20221231.xsd#RelatedPartiesSponsorTrusteeCustodianAndMarketingFeesDetails" roleURI="http://perthmintbullion.com/role/RelatedPartiesSponsorTrusteeCustodianAndMarketingFeesDetails" />
<roleRef xlink:type="simple" xlink:href="aaau-20221231.xsd#SignificantAccountingPoliciesTables" roleURI="http://perthmintbullion.com/role/SignificantAccountingPoliciesTables" />
<roleRef xlink:type="simple" xlink:href="aaau-20221231.xsd#InvestmentInGoldTables" roleURI="http://perthmintbullion.com/role/InvestmentInGoldTables" />
<roleRef xlink:type="simple" xlink:href="aaau-20221231.xsd#SignificantAccountingPoliciesDetails" roleURI="http://perthmintbullion.com/role/SignificantAccountingPoliciesDetails" />
<roleRef xlink:type="simple" xlink:href="aaau-20221231.xsd#FinancialHighlights" roleURI="http://perthmintbullion.com/role/FinancialHighlights" />
<roleRef xlink:type="simple" xlink:href="aaau-20221231.xsd#StatementsOfAssetsAndLiabilities" roleURI="http://perthmintbullion.com/role/StatementsOfAssetsAndLiabilities" />
<roleRef xlink:type="simple" xlink:href="aaau-20221231.xsd#StatementsOfOperations" roleURI="http://perthmintbullion.com/role/StatementsOfOperations" />
<roleRef xlink:type="simple" xlink:href="aaau-20221231.xsd#SchedulesOfInvestments" roleURI="http://perthmintbullion.com/role/SchedulesOfInvestments" />
<roleRef xlink:type="simple" xlink:href="aaau-20221231.xsd#SchedulesOfInvestmentsTables" roleURI="http://perthmintbullion.com/role/SchedulesOfInvestmentsTables" />
<roleRef xlink:type="simple" xlink:href="aaau-20221231.xsd#SignificantAccountingPoliciesScheduleOfChangesInOuncesOfGoldAndRespectiveFairValueParentheticalsDetails" roleURI="http://perthmintbullion.com/role/SignificantAccountingPoliciesScheduleOfChangesInOuncesOfGoldAndRespectiveFairValueParentheticalsDetails" />
<roleRef xlink:type="simple" xlink:href="aaau-20221231.xsd#CoverPage" roleURI="http://perthmintbullion.com/role/CoverPage" />
<arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all" />
<arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default" />
<arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain" />
<arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member" />
<arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension" />
<arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/notAll" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#notAll" />
<definitionLink xlink:type="extended" xlink:role="http://perthmintbullion.com/role/StatementsOfCashFlows">
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementLineItems" xlink:label="loc_us-gaap_StatementLineItems_2147470963" />
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract" xlink:label="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_2147470511" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_2147470963" xlink:to="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_2147470511" use="optional" order="1" />
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_UnrealizedGainLossOnInvestments" xlink:label="loc_us-gaap_UnrealizedGainLossOnInvestments_2147471532" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_2147470511" xlink:to="loc_us-gaap_UnrealizedGainLossOnInvestments_2147471532" use="optional" order="2" />
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RealizedInvestmentGainsLosses" xlink:label="loc_us-gaap_RealizedInvestmentGainsLosses_2147471529" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_2147470511" xlink:to="loc_us-gaap_RealizedInvestmentGainsLosses_2147471529" use="optional" order="3" />
<loc xlink:type="locator" xlink:href="aaau-20221231.xsd#aaau_AdjustmentToCashflowFromOperatingActivitiesSaleMaturityAndCollectionOfInvestments" xlink:label="loc_aaau_AdjustmentToCashflowFromOperatingActivitiesSaleMaturityAndCollectionOfInvestments_2147470980" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AdjustmentsToReconcileNetIncomeLossToCashProvidedByUsedInOperatingActivitiesAbstract_2147470511" xlink:to="loc_aaau_AdjustmentToCashflowFromOperatingActivitiesSaleMaturityAndCollectionOfInvestments_2147470980" use="optional" order="4" />
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInOperatingCapitalAbstract" xlink:label="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_2147470516" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_2147470963" xlink:to="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_2147470516" use="optional" order="5" />
<loc xlink:type="locator" xlink:href="aaau-20221231.xsd#aaau_IncreaseDecreaseInSponsorFeePayable" xlink:label="loc_aaau_IncreaseDecreaseInSponsorFeePayable_2147471536" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInOperatingCapitalAbstract_2147470516" xlink:to="loc_aaau_IncreaseDecreaseInSponsorFeePayable_2147471536" use="optional" order="6" />
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_2147470504" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_2147470500" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_2147470504" use="optional" order="7" />
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SupplementalCashFlowInformationAbstract" xlink:label="loc_us-gaap_SupplementalCashFlowInformationAbstract_2147470519" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_2147470963" xlink:to="loc_us-gaap_SupplementalCashFlowInformationAbstract_2147470519" use="optional" order="8" />
<loc xlink:type="locator" xlink:href="aaau-20221231.xsd#aaau_StockRedeemeedDuringThePeriodOne" xlink:label="loc_aaau_StockRedeemeedDuringThePeriodOne_2147470982" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SupplementalCashFlowInformationAbstract_2147470519" xlink:to="loc_aaau_StockRedeemeedDuringThePeriodOne_2147470982" use="optional" order="9" />
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockIssued1" xlink:label="loc_us-gaap_StockIssued1_2147470981" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SupplementalCashFlowInformationAbstract_2147470519" xlink:to="loc_us-gaap_StockIssued1_2147470981" use="optional" order="10" />
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_2147470500" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_2147470963" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_2147470500" use="optional" order="11" />
<loc xlink:type="locator" xlink:href="aaau-20221231.xsd#aaau_ProceedsFromSaleOfTrustAssetsToPayExpenses1" xlink:label="loc_aaau_ProceedsFromSaleOfTrustAssetsToPayExpenses1_2147471516" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_2147470500" xlink:to="loc_aaau_ProceedsFromSaleOfTrustAssetsToPayExpenses1_2147471516" use="optional" order="12" />
<loc xlink:type="locator" xlink:href="aaau-20221231.xsd#aaau_ExpensesSponsorFeePaid" xlink:label="loc_aaau_ExpensesSponsorFeePaid_2147471537" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivitiesAbstract_2147470500" xlink:to="loc_aaau_ExpensesSponsorFeePaid_2147471537" use="optional" order="13" />
<loc xlink:type="locator" xlink:href="aaau-20221231.xsd#aaau_ReconciliationOfNetIncreaseDecreaseInNetAssetsResultingFromOperationsToNetCashProvidedByUsedInOperatingActivitiesAbstract" xlink:label="loc_aaau_ReconciliationOfNetIncreaseDecreaseInNetAssetsResultingFromOperationsToNetCashProvidedByUsedInOperatingActivitiesAbstract_2147470509" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_2147470963" xlink:to="loc_aaau_ReconciliationOfNetIncreaseDecreaseInNetAssetsResultingFromOperationsToNetCashProvidedByUsedInOperatingActivitiesAbstract_2147470509" use="optional" order="14" />
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetIncomeLoss" xlink:label="loc_us-gaap_NetIncomeLoss_2147470510" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_aaau_ReconciliationOfNetIncreaseDecreaseInNetAssetsResultingFromOperationsToNetCashProvidedByUsedInOperatingActivitiesAbstract_2147470509" xlink:to="loc_us-gaap_NetIncomeLoss_2147470510" use="optional" order="15" />
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseExcludingExchangeRateEffect" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseExcludingExchangeRateEffect_2147470505" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_2147470963" xlink:to="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseExcludingExchangeRateEffect_2147470505" use="optional" order="16" />
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_2147470506" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_2147470963" xlink:to="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_2147470506" use="optional" order="17" />
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementTable" xlink:label="loc_us-gaap_StatementTable_2147470936" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_2147470963" xlink:to="loc_us-gaap_StatementTable_2147470936" xbrldt:contextElement="segment" xbrldt:closed="true" use="optional" order="18" />
<loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_LegalEntityAxis" xlink:label="loc_dei_LegalEntityAxis_2147470960" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_2147470936" xlink:to="loc_dei_LegalEntityAxis_2147470960" use="optional" order="19" />
<loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_EntityDomain" xlink:label="loc_dei_EntityDomain_2147470961" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_2147470960" xlink:to="loc_dei_EntityDomain_2147470961" use="optional" order="20" />
<loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_EntityDomain" xlink:label="loc_dei_EntityDomain_2147470961_default" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_2147470960" xlink:to="loc_dei_EntityDomain_2147470961_default" use="optional" order="21" />
</definitionLink>
<definitionLink xlink:type="extended" xlink:role="http://perthmintbullion.com/role/SchedulesOfInvestmentsSchedulesOfInvestmentsDetails">
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SummaryOfInvestmentHoldingsLineItems" xlink:label="loc_us-gaap_SummaryOfInvestmentHoldingsLineItems_59" />
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ScheduleOfInvestmentsTable" xlink:label="loc_us-gaap_ScheduleOfInvestmentsTable_56" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SummaryOfInvestmentHoldingsLineItems_59" xlink:to="loc_us-gaap_ScheduleOfInvestmentsTable_56" xbrldt:contextElement="segment" xbrldt:closed="true" use="optional" order="1" />
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_CurrencyAxis" xlink:label="loc_srt_CurrencyAxis_57" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfInvestmentsTable_56" xlink:to="loc_srt_CurrencyAxis_57" use="optional" order="2" />
<loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/currency/2022/currency-2022.xsd#currency_AllCurrenciesDomain" xlink:label="loc_currency_AllCurrenciesDomain_58" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CurrencyAxis_57" xlink:to="loc_currency_AllCurrenciesDomain_58" use="optional" order="3" />
<loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/currency/2022/currency-2022.xsd#currency_AllCurrenciesDomain" xlink:label="loc_currency_AllCurrenciesDomain_58_default" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CurrencyAxis_57" xlink:to="loc_currency_AllCurrenciesDomain_58_default" use="optional" order="4" />
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InformationByCategoryOfDebtSecurityAxis" xlink:label="loc_us-gaap_InformationByCategoryOfDebtSecurityAxis_844108" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfInvestmentsTable_56" xlink:to="loc_us-gaap_InformationByCategoryOfDebtSecurityAxis_844108" use="optional" order="5" />
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherThanTemporaryImpairmentCreditLossesRecognizedInEarningsCategoriesOfInvestmentsDomain" xlink:label="loc_us-gaap_OtherThanTemporaryImpairmentCreditLossesRecognizedInEarningsCategoriesOfInvestmentsDomain_844109" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_InformationByCategoryOfDebtSecurityAxis_844108" xlink:to="loc_us-gaap_OtherThanTemporaryImpairmentCreditLossesRecognizedInEarningsCategoriesOfInvestmentsDomain_844109" use="optional" order="6" />
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherThanTemporaryImpairmentCreditLossesRecognizedInEarningsCategoriesOfInvestmentsDomain" xlink:label="loc_us-gaap_OtherThanTemporaryImpairmentCreditLossesRecognizedInEarningsCategoriesOfInvestmentsDomain_844109_default" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_InformationByCategoryOfDebtSecurityAxis_844108" xlink:to="loc_us-gaap_OtherThanTemporaryImpairmentCreditLossesRecognizedInEarningsCategoriesOfInvestmentsDomain_844109_default" use="optional" order="7" />
<loc xlink:type="locator" xlink:href="aaau-20221231.xsd#aaau_LiabilitiesInExcessOfOtherAssetsMember" xlink:label="loc_aaau_LiabilitiesInExcessOfOtherAssetsMember_844117" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherThanTemporaryImpairmentCreditLossesRecognizedInEarningsCategoriesOfInvestmentsDomain_844109" xlink:to="loc_aaau_LiabilitiesInExcessOfOtherAssetsMember_844117" use="optional" order="8" />
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InvestmentsMember" xlink:label="loc_us-gaap_InvestmentsMember_844113" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherThanTemporaryImpairmentCreditLossesRecognizedInEarningsCategoriesOfInvestmentsDomain_844109" xlink:to="loc_us-gaap_InvestmentsMember_844113" use="optional" order="9" />
<loc xlink:type="locator" xlink:href="aaau-20221231.xsd#aaau_NetAssetsMember" xlink:label="loc_aaau_NetAssetsMember_844119" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherThanTemporaryImpairmentCreditLossesRecognizedInEarningsCategoriesOfInvestmentsDomain_844109" xlink:to="loc_aaau_NetAssetsMember_844119" use="optional" order="10" />
<loc xlink:type="locator" xlink:href="aaau-20221231.xsd#aaau_GoldBullionMember" xlink:label="loc_aaau_GoldBullionMember_844111" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherThanTemporaryImpairmentCreditLossesRecognizedInEarningsCategoriesOfInvestmentsDomain_844109" xlink:to="loc_aaau_GoldBullionMember_844111" use="optional" order="11" />
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InvestmentOwnedAtFairValue" xlink:label="loc_us-gaap_InvestmentOwnedAtFairValue_63" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SummaryOfInvestmentHoldingsLineItems_59" xlink:to="loc_us-gaap_InvestmentOwnedAtFairValue_63" use="optional" order="12" />
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InvestmentOwnedPercentOfNetAssets" xlink:label="loc_us-gaap_InvestmentOwnedPercentOfNetAssets_64" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SummaryOfInvestmentHoldingsLineItems_59" xlink:to="loc_us-gaap_InvestmentOwnedPercentOfNetAssets_64" use="optional" order="13" />
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InvestmentOwnedBalanceOtherMeasure" xlink:label="loc_us-gaap_InvestmentOwnedBalanceOtherMeasure_61" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SummaryOfInvestmentHoldingsLineItems_59" xlink:to="loc_us-gaap_InvestmentOwnedBalanceOtherMeasure_61" use="optional" order="14" />
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InvestmentOwnedAtCost" xlink:label="loc_us-gaap_InvestmentOwnedAtCost_62" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SummaryOfInvestmentHoldingsLineItems_59" xlink:to="loc_us-gaap_InvestmentOwnedAtCost_62" use="optional" order="15" />
</definitionLink>
<definitionLink xlink:type="extended" xlink:role="http://perthmintbullion.com/role/OrganizationDetails">
<loc xlink:type="locator" xlink:href="aaau-20221231.xsd#aaau_OrganizationDetailsLineItems" xlink:label="loc_aaau_OrganizationDetailsLineItems_110" />
<loc xlink:type="locator" xlink:href="aaau-20221231.xsd#aaau_SharesIssuedInBasketInExchangeForGold" xlink:label="loc_aaau_SharesIssuedInBasketInExchangeForGold_113" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_aaau_OrganizationDetailsLineItems_110" xlink:to="loc_aaau_SharesIssuedInBasketInExchangeForGold_113" use="optional" order="1" />
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperationsCommencedDate1" xlink:label="loc_us-gaap_OperationsCommencedDate1_116" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_aaau_OrganizationDetailsLineItems_110" xlink:to="loc_us-gaap_OperationsCommencedDate1_116" use="optional" order="2" />
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StatementTable" xlink:label="loc_us-gaap_StatementTable_105" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_aaau_OrganizationDetailsLineItems_110" xlink:to="loc_us-gaap_StatementTable_105" xbrldt:contextElement="segment" xbrldt:closed="true" use="optional" order="3" />
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeAxis" xlink:label="loc_srt_RangeAxis_108" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_105" xlink:to="loc_srt_RangeAxis_108" use="optional" order="4" />
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeMember" xlink:label="loc_srt_RangeMember_109" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_108" xlink:to="loc_srt_RangeMember_109" use="optional" order="5" />
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_RangeMember" xlink:label="loc_srt_RangeMember_109_default" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_108" xlink:to="loc_srt_RangeMember_109_default" use="optional" order="6" />
<loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2022/elts/srt-2022.xsd#srt_MinimumMember" xlink:label="loc_srt_MinimumMember_112" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_109" xlink:to="loc_srt_MinimumMember_112" use="optional" order="7" />
<loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_LegalEntityAxis" xlink:label="loc_dei_LegalEntityAxis_106" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_105" xlink:to="loc_dei_LegalEntityAxis_106" use="optional" order="8" />
<loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_EntityDomain" xlink:label="loc_dei_EntityDomain_107" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_106" xlink:to="loc_dei_EntityDomain_107" use="optional" order="9" />
<loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2022/dei-2022.xsd#dei_EntityDomain" xlink:label="loc_dei_EntityDomain_107_default" />
<definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_106" xlink:to="loc_dei_EntityDomain_107_default" use="optional" order="10" />
</definitionLink>
</linkbase>
