Exhibit 8.1

Subsidiaries of Galapagos NV

In liquidatio |  |
Name of Subsidiary |  | Jurisdiction of Incorporation or Organization
Galapagos B.V. |  | The Netherlands
BioFocus DPI AG in liquidation |  | Switzerland
Galapagos Biotech Ltd. |  | United Kingdom
Galapagos SASU |  | France
Fidelta d.o.o. |  | Croatia
Galapagos, Inc. |  | United States
Xenometrix, Inc. |  | United States
Galapagos GmbH |  | Switzerland
Galapagos Real Estate 1 BV |  | Belgium
Galapagos Real Estate 2 BV |  | Belgium
Galapagos Biopharma Belgium BV |  | Belgium
Galapagos Real Estate Netherlands B.V. |  | Netherlands
Galapagos Biopharma Netherlands B.V. |  | Netherlands
Galapagos Biopharma Spain S.L.U. |  | Spain
Galapagos Biopharma Italy S.r.l. |  | Italy
Galapagos Biopharma Germany GmbH |  | Germany

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