<SUBMISSION>
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<PERIOD>20140327
<FILING-DATE>20140327
<DATE-OF-FILING-DATE-CHANGE>20140327
<ISSUER>
<COMPANY-DATA>
<CONFORMED-NAME>2U, Inc.
<CIK>0001459417
<ASSIGNED-SIC>7372
<IRS-NUMBER>262335939
<STATE-OF-INCORPORATION>DE
<FISCAL-YEAR-END>1231
</COMPANY-DATA>
<BUSINESS-ADDRESS>
<STREET1>8201 CORPORATE DRIVE, SUITE 110
<CITY>LANDOVER
<STATE>MD
<ZIP>20785
<PHONE>240-487-3991
</BUSINESS-ADDRESS>
<MAIL-ADDRESS>
<STREET1>8201 CORPORATE DRIVE, SUITE 110
<CITY>LANDOVER
<STATE>MD
<ZIP>20785
</MAIL-ADDRESS>
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<FORMER-CONFORMED-NAME>2tor, Inc.
<DATE-CHANGED>20090324
</FORMER-COMPANY>
</ISSUER>
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<BUSINESS-ADDRESS>
<STREET1>92 HAYDEN AVE
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<STATE>MA
<ZIP>02421
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<CITY>LEXINGTON
<STATE>MA
<ZIP>02421
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<REPORTING-OWNER>
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<BUSINESS-ADDRESS>
<STREET1>ONE BROADWAY, 16TH FLOOR
<CITY>CAMBRIDGE
<STATE>MA
<ZIP>02142
<PHONE>617-401-4500
</BUSINESS-ADDRESS>
<MAIL-ADDRESS>
<STREET1>ONE BROADWAY, 16TH FLOOR
<CITY>CAMBRIDGE
<STATE>MA
<ZIP>02142
</MAIL-ADDRESS>
</REPORTING-OWNER>
<REPORTING-OWNER>
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<CONFORMED-NAME>Highland Entrepreneurs Fund VII Limited Partnership
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<BUSINESS-ADDRESS>
<STREET1>92 HAYDEN AVENUE
<CITY>LEXINGTON
<STATE>MA
<ZIP>02421
<PHONE>781-861-5500
</BUSINESS-ADDRESS>
<MAIL-ADDRESS>
<STREET1>92 HAYDEN AVENUE
<CITY>LEXINGTON
<STATE>MA
<ZIP>02421
</MAIL-ADDRESS>
</REPORTING-OWNER>
<REPORTING-OWNER>
<OWNER-DATA>
<CONFORMED-NAME>Highland Capital Partners VII-C LP
<CIK>0001343673
</OWNER-DATA>
<FILING-VALUES>
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<ACT>34
<FILE-NUMBER>001-36376
<FILM-NUMBER>14723071
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<BUSINESS-ADDRESS>
<STREET1>92 HAYDEN AVE
<CITY>LEXINGTON
<STATE>MA
<ZIP>02421
</BUSINESS-ADDRESS>
<MAIL-ADDRESS>
<STREET1>92 HAYDEN AVE
<CITY>LEXINGTON
<STATE>MA
<ZIP>02421
</MAIL-ADDRESS>
</REPORTING-OWNER>
<REPORTING-OWNER>
<OWNER-DATA>
<CONFORMED-NAME>HIGHLAND CAPITAL PARTNERS VII-B L P
<CIK>0001343244
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<BUSINESS-ADDRESS>
<STREET1>92 HAYDEN AVE
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<STATE>MA
<ZIP>02421
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<MAIL-ADDRESS>
<STREET1>92 HAYDEN AVE
<CITY>LEXINGTON
<STATE>MA
<ZIP>02421
</MAIL-ADDRESS>
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<REPORTING-OWNER>
<OWNER-DATA>
<CONFORMED-NAME>Highland Management Partners VII, LLC
<CIK>0001554026
<STATE-OF-INCORPORATION>DE
<FISCAL-YEAR-END>1231
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<BUSINESS-ADDRESS>
<STREET1>ONE BROADWAY, 16TH FLOOR
<CITY>CAMBRIDGE
<STATE>MA
<ZIP>02142
<PHONE>617-401-4500
</BUSINESS-ADDRESS>
<MAIL-ADDRESS>
<STREET1>ONE BROADWAY, 16TH FLOOR
<CITY>CAMBRIDGE
<STATE>MA
<ZIP>02142
</MAIL-ADDRESS>
</REPORTING-OWNER>
<DOCUMENT>
<TYPE>3
<SEQUENCE>1
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<DESCRIPTION>3
<TEXT>
<XML>
<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0206</schemaVersion>

    <documentType>3</documentType>

    <periodOfReport>2014-03-27</periodOfReport>

    <noSecuritiesOwned>0</noSecuritiesOwned>

    <issuer>
        <issuerCik>0001459417</issuerCik>
        <issuerName>2U, Inc.</issuerName>
        <issuerTradingSymbol>TWOU</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001554026</rptOwnerCik>
            <rptOwnerName>Highland Management Partners VII, LLC</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O HIGHLAND CAPITAL PARTNERS, ONE</rptOwnerStreet1>
            <rptOwnerStreet2>BROADWAY, 16TH FLOOR</rptOwnerStreet2>
            <rptOwnerCity>CAMBRIDGE</rptOwnerCity>
            <rptOwnerState>MA</rptOwnerState>
            <rptOwnerZipCode>02142</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001343244</rptOwnerCik>
            <rptOwnerName>HIGHLAND CAPITAL PARTNERS VII-B L P</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O HIGHLAND CAPITAL PARTNERS, ONE</rptOwnerStreet1>
            <rptOwnerStreet2>BROADWAY, 16TH FLOOR</rptOwnerStreet2>
            <rptOwnerCity>CAMBRIDGE</rptOwnerCity>
            <rptOwnerState>MA</rptOwnerState>
            <rptOwnerZipCode>02142</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001343673</rptOwnerCik>
            <rptOwnerName>Highland Capital Partners VII-C LP</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O HIGHLAND CAPITAL PARTNERS, ONE</rptOwnerStreet1>
            <rptOwnerStreet2>BROADWAY, 16TH FLOOR</rptOwnerStreet2>
            <rptOwnerCity>CAMBRIDGE</rptOwnerCity>
            <rptOwnerState>MA</rptOwnerState>
            <rptOwnerZipCode>02142</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001357840</rptOwnerCik>
            <rptOwnerName>Highland Entrepreneurs Fund VII Limited Partnership</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O HIGHLAND CAPITAL PARTNERS, ONE</rptOwnerStreet1>
            <rptOwnerStreet2>BROADWAY, 16TH FLOOR</rptOwnerStreet2>
            <rptOwnerCity>CAMBRIDGE</rptOwnerCity>
            <rptOwnerState>MA</rptOwnerState>
            <rptOwnerZipCode>02142</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001554027</rptOwnerCik>
            <rptOwnerName>Highland Management Partners VII Limited Partnership</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O HIGHLAND CAPITAL PARTNERS, ONE</rptOwnerStreet1>
            <rptOwnerStreet2>BROADWAY, 16TH FLOOR</rptOwnerStreet2>
            <rptOwnerCity>CAMBRIDGE</rptOwnerCity>
            <rptOwnerState>MA</rptOwnerState>
            <rptOwnerZipCode>02142</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001343678</rptOwnerCik>
            <rptOwnerName>Highland Capital Partners VII LP</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O HIGHLAND CAPITAL PARTNERS, ONE</rptOwnerStreet1>
            <rptOwnerStreet2>BROADWAY, 16TH FLOOR</rptOwnerStreet2>
            <rptOwnerCity>CAMBRIDGE</rptOwnerCity>
            <rptOwnerState>MA</rptOwnerState>
            <rptOwnerZipCode>02142</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <derivativeTable>
        <derivativeHolding>
            <securityTitle>
                <value>Series B Preferred Stock</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <footnoteId id="F5"/>
                <footnoteId id="F6"/>
            </conversionOrExercisePrice>
            <exerciseDate>
                <footnoteId id="F5"/>
                <footnoteId id="F6"/>
            </exerciseDate>
            <expirationDate>
                <footnoteId id="F5"/>
                <footnoteId id="F6"/>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>2747352</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>See footnote</value>
                    <footnoteId id="F1"/>
                    <footnoteId id="F2"/>
                    <footnoteId id="F3"/>
                    <footnoteId id="F4"/>
                </natureOfOwnership>
            </ownershipNature>
        </derivativeHolding>
        <derivativeHolding>
            <securityTitle>
                <value>Series C Preferred Stock</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <footnoteId id="F5"/>
                <footnoteId id="F6"/>
            </conversionOrExercisePrice>
            <exerciseDate>
                <footnoteId id="F5"/>
                <footnoteId id="F6"/>
            </exerciseDate>
            <expirationDate>
                <footnoteId id="F5"/>
                <footnoteId id="F6"/>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>475899</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>See footnote</value>
                    <footnoteId id="F1"/>
                    <footnoteId id="F2"/>
                    <footnoteId id="F3"/>
                    <footnoteId id="F4"/>
                </natureOfOwnership>
            </ownershipNature>
        </derivativeHolding>
        <derivativeHolding>
            <securityTitle>
                <value>Series D Preferred Stock</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <footnoteId id="F5"/>
                <footnoteId id="F6"/>
            </conversionOrExercisePrice>
            <exerciseDate>
                <footnoteId id="F5"/>
                <footnoteId id="F6"/>
            </exerciseDate>
            <expirationDate>
                <footnoteId id="F5"/>
                <footnoteId id="F6"/>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>319914</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>See footnote</value>
                    <footnoteId id="F1"/>
                    <footnoteId id="F2"/>
                    <footnoteId id="F3"/>
                    <footnoteId id="F4"/>
                </natureOfOwnership>
            </ownershipNature>
        </derivativeHolding>
    </derivativeTable>

    <footnotes>
        <footnote id="F1">The securities are held by Highland Capital Partners VII Limited Partnership (&quot;HCP VII&quot;).  Highland Management Partners VII, LLC (&quot;HMP VII LLC&quot;) is the general partner of Highland Management Partnership VII Limited Partnership (&quot;HMP VII LP&quot;), which is the general partner of HCP VII.  Each of HMP VII LP and HMP VII LLC disclaims beneficial ownership of all shares held by HCP VII except to the extent, if any, of such entity's pecuniary interest therein.</footnote>
        <footnote id="F2">The securities are held by Highland Capital Partners VII-B Limited Partnership (&quot;HCP VII-B&quot;).  HMP VII LP is the general partner of HCP VII-B.  Each of HMP VII LP and HMP VII LLC disclaims beneficial ownership of all shares held by HCP VII-B except to the extent, if any, of such entity's pecuniary interest therein.</footnote>
        <footnote id="F3">The securities are held by Highland Capital Partners VII-C Limited Partnership (&quot;HCP VII-C&quot;).  HMP VII LP is the general partner of HCP VII-C.  Each of HMP VII LP and HMP VII LLC disclaims beneficial ownership of all shares held by HCP VII-C except to the extent, if any, of such entity's pecuniary interest therein.</footnote>
        <footnote id="F4">The securities are held by Highland Entrepreneurs' Fund VII Limited Partnership (&quot;HEF VII&quot;).  HMP VII LP is the general partner of HEF VII.  Each of HMP VII LP and HMP VII LLC disclaims beneficial ownership of all shares held by HEF VII except to the extent, if any, of such entity's pecuniary interest therein.</footnote>
        <footnote id="F5">HCP VII is the holder of record of 1,689,070 shares of Series B Preferred Stock, 292,583 shares of Series C Preferred Stock and 196,683 shares of Series D Preferred Stock, which are convertible into 2,178,336 shares of Common Stock.  HCP VII-B is the holder of record of 409,294 shares of Series B Preferred Stock, 70,898, shares of Series C Preferred Stock and 47,660 shares of Series D Preferred Stock, which are convertible into 527,852 shares of Common Stock. HCP VII-C is the holder of record of 596,062 shares of Series B Preferred Stock, 103,250 shares of Series C Preferred Stock and 69,408 shares of Series D Preferred Stock, which are convertible into 768,720 shares of Common Stock.  The Preferred Stock will convert into Common Stock upon the closing of the Issuer's initial public offering on a one-for-one basis, and has no expiration date.</footnote>
        <footnote id="F6">HEF VII is the holder of record of 52,926 shares of Series B Preferred Stock, 9,168 shares of Series C Preferred Stock and 6,163 shares of Series D Preferred Stock, which are convertible into 68,257 shares of Common Stock.  The Preferred Stock will convert into Common Stock upon the closing of the Issuer's initial public offering on a one-for-one basis, and has no expiration date.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ Paul A. Maeder, Authorized Manager of Highland Management Partners VII, LLC</signatureName>
        <signatureDate>2014-03-27</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ Paul A. Maeder, Authorized Manager of Highland Management Partners VII, LLC, the general partner of Highland Management Partners VII Limited Partnership</signatureName>
        <signatureDate>2014-03-27</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ Paul A. Maeder, Authorized Manager of Highland Management Partners VII, LLC, the general partner of Highland Management Partners VII Limited Partnership, the general partner of Highland Capital Partners VII Limited Partnership</signatureName>
        <signatureDate>2014-03-27</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ Paul A. Maeder, Authorized Manager of Highland Management Partners VII, LLC, the general partner of Highland Management Partners VII Limited Partnership, the general partner of Highland Capital Partners VII-B Limited Partnership</signatureName>
        <signatureDate>2014-03-27</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ Paul A. Maeder, Authorized Manager of Highland Management Partners VII, LLC, the general partner of Highland Management Partners VII Limited Partnership, the general partner of Highland Capital Partners VII-C Limited Partnership</signatureName>
        <signatureDate>2014-03-27</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ Paul A. Maeder, Authorized Manager of Highland Management Partners VII, LLC, the general partner of Highland Management Partners VII Limited Partnership, the general partner of Highland Entrepreneurs' Fund VII Limited Partnership</signatureName>
        <signatureDate>2014-03-27</signatureDate>
    </ownerSignature>
</ownershipDocument>
</XML>
</TEXT>
</DOCUMENT>
</SUBMISSION>
