<PAGE>
                                                                    EXHIBIT 25.1
================================================================================

                       SECURITIES AND EXCHANGE COMMISSION
                             WASHINGTON, D.C. 20549
                           --------------------------

                                    FORM T-1

                         STATEMENT OF ELIGIBILITY UNDER
                      THE TRUST INDENTURE ACT OF 1939 OF A
                    CORPORATION DESIGNATED TO ACT AS TRUSTEE
               Check if an Application to Determine Eligibility of
                     a Trustee Pursuant to Section 305(b)(2)

             -------------------------------------------------------

                         U.S. BANK NATIONAL ASSOCIATION
               (Exact name of Trustee as specified in its charter)

                                   31-0841368
                       I.R.S. Employer Identification No.

         180 East Fifth Street
         St. Paul, Minnesota                                            55101
(Address of principal executive offices)                              (Zip Code)

                                 James Kowalski
                         U.S. Bank National Association
                         535 Griswold Street, Suite 550
                                Detroit, MI 48226
                                 (313) 234-4700
            (Name, address and telephone number of agent for service)

                        CHILDTIME LEARNING CENTERS, INC.
                     (Issuer with respect to the Securities)

<TABLE>
<S><C>
                         Michigan                                                    38-3261854

(State or other jurisdiction of incorporation or organization)         (I.R.S. Employer Identification No.)
</TABLE>


           38345 West Nine Mile Road                                     48335
                  Suite 100
         Farmington Hills, Michigan
  (Address of Principal Executive Offices)                            (Zip Code)


                         15% SUBORDINATED NOTES DUE 2008
                       (TITLE OF THE INDENTURE SECURITIES)


================================================================================




<PAGE>




                                    FORM T-1

ITEM 1.      GENERAL INFORMATION.  Furnish the following information as to the
             Trustee.

              a)    Name and address of each examining or supervising authority
                    to which it is subject.
                           Comptroller of the Currency
                           Washington, D.C.

              b)  Whether it is authorized to exercise corporate trust powers.
                           Yes

ITEM 2.     AFFILIATIONS WITH OBLIGOR. If the obligor is an affiliate of the
            Trustee, describe each such affiliation.

                  None

ITEMS 3-15  Items 3-15 are not applicable because to the best of the Trustee's
            knowledge, the obligor is not in default under any Indenture for
            which the Trustee acts as Trustee.

ITEM 16.    LIST OF EXHIBITS: List below all exhibits filed as a part of this
            statement of eligibility and qualification.

              1.   A copy of the Articles of Association of the Trustee.*

              2.   A copy of the certificate of authority of the Trustee to
                   commence business.*

              3.   A copy of the certificate of authority of the Trustee to
                   exercise corporate trust powers.*

              4.   A copy of the existing bylaws of the Trustee.*

              5.   A copy of each Indenture referred to in Item 4. Not
                   applicable.

              6.   The consent of the Trustee required by Section 321(b) of the
                   Trust Indenture Act of 1939, attached as Exhibit 6.

              7.   Report of Condition of the Trustee as of September 30, 2002,
                   published pursuant to law or the requirements of its
                   supervising or examining authority, attached as Exhibit 7.



       * Incorporated by reference to Registration Number 333-67188.


                                       2


<PAGE>




                                      NOTE

         The answers to this statement insofar as such answers relate to what
persons have been underwriters for any securities of the obligors within three
years prior to the date of filing this statement, or what persons are owners of
10% or more of the voting securities of the obligors, or affiliates, are based
upon information furnished to the Trustee by the obligors. While the Trustee has
no reason to doubt the accuracy of any such information, it cannot accept any
responsibility therefor.



                                    SIGNATURE

         Pursuant to the requirements of the Trust Indenture Act of 1939, as
amended, the Trustee, U.S. BANK NATIONAL ASSOCIATION, a national banking
association organized and existing under the laws of the United States of
America, has duly caused this statement of eligibility and qualification to be
signed on its behalf by the undersigned, thereunto duly authorized, all in the
City of Detroit, State of Michigan on the 20th day of February, 2003.

                              U.S. BANK NATIONAL ASSOCIATION

                              By:      /s/ James Kowalski
                                       -----------------------------------
                                       James Kowalski
                                       Assistant Vice President




By:      /s/ Susan T. Payne
         -----------------------------------
         Susan T. Payne
         Vice President





                                       3

<PAGE>




                                    EXHIBIT 6

                                     CONSENT


         In accordance with Section 321(b) of the Trust Indenture Act of 1939,
the undersigned, U.S. BANK NATIONAL ASSOCIATION hereby consents that reports of
examination of the undersigned by Federal, State, Territorial or District
authorities may be furnished by such authorities to the Securities and Exchange
Commission upon its request therefor.


Dated:  February 20, 2003


                               U.S. BANK NATIONAL ASSOCIATION


                               By:      /s/ James Kowalski
                                        -----------------------------
                                        James Kowalski
                                        Assistant Vice President




By:      /s/ Susan T. Payne
         -----------------------------------
         Susan T. Payne
         Vice President




                                       4

<PAGE>






                                    EXHIBIT 7
                         U.S. BANK NATIONAL ASSOCIATION
                        STATEMENT OF FINANCIAL CONDITION
                                 AS OF 9/30/2002

                                    ($000'S)

<TABLE>
<CAPTION>
                                                                          9/30/2002
                                                                        ------------
<S>                                                                       <C>
ASSETS
     Cash and Due From Depository Institutions                            $8,809,794
     Federal Reserve Stock                                                         0
     Securities                                                           28,156,313
     Federal Funds                                                           975,986
     Loans & Lease Financing Receivables                                 111,491,144
     Fixed Assets                                                          1,357,049
     Intangible Assets                                                     8,242,263
     Other Assets                                                          7,510,862
                                                                        ------------
         TOTAL ASSETS                                                   $166,543,411

LIABILITIES
     Deposits                                                           $112,901,360
     Fed Funds                                                             2,319,887
     Treasury Demand Notes                                                         0
     Trading Liabilities                                                     285,504
     Other Borrowed Money                                                 20,829,386
     Acceptances                                                             137,242
     Subordinated Notes and Debentures                                     5,696,195
     Other Liabilities                                                     5,198,418
                                                                        ------------
     TOTAL LIABILITIES                                                  $147,367,992

EQUITY
     Minority Interest in Subsidiaries                                      $990,010
     Common and Preferred Stock                                               18,200
     Surplus                                                              11,312,077
     Undivided Profits                                                     6,855,132
                                                                        ------------
         TOTAL EQUITY CAPITAL                                            $19,175,419

TOTAL LIABILITIES AND EQUITY CAPITAL                                    $166,543,411
</TABLE>

To the best of the undersigned's determination, as of the date hereof, the above
financial information is true and correct.


U.S. BANK NATIONAL ASSOCIATION

By:      /s/ James Kowalski
         -----------------------
         Assistant Vice President

Date:  February 20, 2003




                                       5

